diff --git a/dbm-ui/frontend/src/locales/zh-cn.json b/dbm-ui/frontend/src/locales/zh-cn.json
index 851e746f69..dd25416b57 100644
--- a/dbm-ui/frontend/src/locales/zh-cn.json
+++ b/dbm-ui/frontend/src/locales/zh-cn.json
@@ -3711,5 +3711,6 @@
   "删除规则后将不能恢复，请谨慎操作": "删除规则后将不能恢复，请谨慎操作",
   "请输入访问DB名_以字母开头_支持字母_数字_下划线_多个使用英文逗号_分号或换行分隔": "请输入访问DB名，以字母开头，支持字母，数字，下划线。多个使用英文逗号、分号或换行分隔",
   "单据协助设置": "单据协助设置",
+  "主域名，从域名，单节点必须分开查询": "主域名，从域名，单节点必须分开查询",
   "这行勿动！新增翻译请在上一行添加！": ""
 }
diff --git a/dbm-ui/frontend/src/services/source/dbbase.ts b/dbm-ui/frontend/src/services/source/dbbase.ts
index 6c16badaf8..c751f58c63 100644
--- a/dbm-ui/frontend/src/services/source/dbbase.ts
+++ b/dbm-ui/frontend/src/services/source/dbbase.ts
@@ -42,7 +42,7 @@ export function filterClusters<
     master_domain: string;
     status: string;
   },
->(params: { bk_biz_id: number; exact_domain?: string; cluster_ids?: string }) {
+>(params: { bk_biz_id: number; exact_domain?: string; cluster_ids?: string; domain?: string }) {
   return http.get<T[]>(`${path}/filter_clusters/`, params);
 }
 
diff --git a/dbm-ui/frontend/src/views/permission-retrieve/components/options/components/item/Domain.vue b/dbm-ui/frontend/src/views/permission-retrieve/components/options/components/item/Domain.vue
index 839e69d669..0c2896a91d 100644
--- a/dbm-ui/frontend/src/views/permission-retrieve/components/options/components/item/Domain.vue
+++ b/dbm-ui/frontend/src/views/permission-retrieve/components/options/components/item/Domain.vue
@@ -133,6 +133,16 @@
         return ipList.length <= 20;
       },
     },
+    {
+      message: t('主域名，从域名，单节点必须分开查询'),
+      validator: () => {
+        if (props.accountType === AccountTypes.MYSQL) {
+          const nonEmptyList = Object.values(selectedClusters.value).filter((selectItem) => selectItem.length > 0);
+          return nonEmptyList.length <= 1;
+        }
+        return true;
+      },
+    },
   ];
 
   const clusterSelectorShow = ref(false);
@@ -142,6 +152,12 @@
   const disableClusterSubmitMethod = (clusterList: string[]) =>
     clusterList.length <= 20 ? false : t('至多n台', { n: 20 });
 
+  const updateClusterInfo = () => {
+    const clusterList = Object.values(selectedClusters.value).find((clusterList) => clusterList.length > 0);
+    clusterType.value = (clusterList?.[0].cluster_type || ClusterTypes.TENDBSINGLE) as ClusterTypes;
+    isMaster.value = !selectedClusters.value?.tendbhaSlave?.length;
+  };
+
   const handleClusterSelectorChange = (selected: Record<string, Array<SelectorModelType>>) => {
     selectedClusters.value = selected;
     const domainList = Object.keys(selected).reduce<string[]>(
@@ -149,10 +165,7 @@
       [],
     );
     modelValue.value = domainList.join(',');
-
-    const clusterList = Object.values(selected).find((clusterList) => clusterList.length > 0);
-    clusterType.value = (clusterList?.[0].cluster_type || ClusterTypes.TENDBSINGLE) as ClusterTypes;
-    isMaster.value = !selectedClusters.value?.tendbhaSlave?.length;
+    updateClusterInfo();
 
     emits('change');
   };
@@ -217,9 +230,9 @@
     const deleteSet = new Set(deleteList);
 
     if (addList.length) {
-      filterClusters({
+      filterClusters<SelectorModelType>({
         bk_biz_id: window.PROJECT_CONFIG.BIZ_ID,
-        exact_domain: addList.join(','),
+        domain: addList.join(','),
       }).then((clusterResultList) => {
         // 删除时，删去已选集群
         deleteSelectedCluster(deleteSet);
@@ -230,10 +243,7 @@
           selected[clusterItem.cluster_type] = selected[clusterItem.cluster_type].concat(clusterItem);
         });
         selectedClusters.value = selected;
-
-        const clusterList = Object.values(selected).find((clusterList) => clusterList.length > 0);
-        clusterType.value = (clusterList?.[0].cluster_type || ClusterTypes.TENDBSINGLE) as ClusterTypes;
-        isMaster.value = !selectedClusters.value?.tendbhaSlave?.length;
+        updateClusterInfo();
 
         nextTick(() => {
           emits('change');
@@ -253,6 +263,7 @@
         const clusterList = selectedClusters.value[key];
         selectedClusters.value[key] = clusterList.filter((clusterItem) => !deleteSet.has(clusterItem.master_domain));
       });
+      updateClusterInfo();
     }
   };