diff --git a/docs/usage.md b/docs/usage.md index b8b811f..c8b07b5 100755 --- a/docs/usage.md +++ b/docs/usage.md @@ -147,23 +147,23 @@ Install mamba bash Mambaforge-$(uname)-$(uname -m).sh -b -p $HOME/mambaforge ``` Add mamba to your PATH -`export PATH=$PATH:/your/mamba/directory/path/bin` +```export PATH=$PATH:/your/mamba/directory/path/bin +``` Install Kraken2 -`mamba install -c bioconda kraken2 -y` +```mamba install -c bioconda kraken2 -y +``` Copy all your fasta files to a directory, in this case, Fasta_dir -Now add the add to library command to add your fasta files to the kraken database +Now use the `add to library`` command to add your fasta files to the kraken database ``` for fasta in Fasta_dir/* ; do kraken2-build --add-to-library $fasta --db orthopox_kdb --threads $THREADS; done ``` Download the reference NCBI taxonomy files -``` -kraken2-build --download-taxonomy --db orthopox_kdb --threads $THREADS +```kraken2-build --download-taxonomy --db orthopox_kdb --threads $THREADS ``` Now build the database -``` -kraken2-build --build --db orthopox_kdb --threads $THREADS +```kraken2-build --build --db orthopox_kdb --threads $THREADS ``` ## Running the pipeline