From cb4a070a13e2018a118c00db118087b93db067a4 Mon Sep 17 00:00:00 2001 From: Anthony Date: Wed, 9 Jun 2021 19:43:48 +0200 Subject: [PATCH] Update pipeline to download and use SRAs from GEO (#1) * rework from sra-download * linting * through sras and trimming * through making rsem-star reference * adding velocity calculations * working through making a_obs * copying new results * finish pipeline through making dataframes * comment out testing * update input link * add kinase results to all to prompt full run --- .gitignore | 6 +- .gitmodules | 3 + README.md | 12 +- SingleCellProteogenomics | 1 + Snakefile | 65 - environment.yaml | 16 - {data => resources}/ERCC.fa | 0 resources/GSE146773_series_matrix.txt | 94 ++ rules/quant.smk | 102 -- workflow/Snakefile | 33 + workflow/config/SraAccList.yaml | 1154 +++++++++++++++++ workflow/envs/downloads.yaml | 12 + workflow/envs/environment.yaml | 21 + workflow/envs/quant.yaml | 9 + workflow/envs/setup.yaml | 4 + workflow/envs/velo.yaml | 5 + workflow/rules/align.smk | 137 ++ workflow/rules/downloads.smk | 99 ++ workflow/rules/quant.smk | 98 ++ workflow/rules/singlecellproteogenomics.smk | 93 ++ workflow/rules/velo.smk | 39 + {scripts => workflow/scripts}/filter_fasta.py | 7 +- .../scripts}/fix_gff3_for_rsem.py | 14 +- workflow/scripts/make_a_obs.py | 35 + .../scripts}/make_rsem_dataframe.py | 37 +- 25 files changed, 1892 insertions(+), 204 deletions(-) create mode 100644 .gitmodules create mode 160000 SingleCellProteogenomics delete mode 100644 Snakefile delete mode 100644 environment.yaml rename {data => resources}/ERCC.fa (100%) create mode 100644 resources/GSE146773_series_matrix.txt delete mode 100644 rules/quant.smk create mode 100644 workflow/Snakefile create mode 100644 workflow/config/SraAccList.yaml create mode 100644 workflow/envs/downloads.yaml create mode 100644 workflow/envs/environment.yaml create mode 100644 workflow/envs/quant.yaml create mode 100644 workflow/envs/setup.yaml create mode 100644 workflow/envs/velo.yaml create mode 100644 workflow/rules/align.smk create mode 100644 workflow/rules/downloads.smk create mode 100644 workflow/rules/quant.smk create mode 100644 workflow/rules/singlecellproteogenomics.smk create mode 100644 workflow/rules/velo.smk rename {scripts => workflow/scripts}/filter_fasta.py (66%) rename {scripts => workflow/scripts}/fix_gff3_for_rsem.py (64%) create mode 100644 workflow/scripts/make_a_obs.py rename {scripts => workflow/scripts}/make_rsem_dataframe.py (67%) diff --git a/.gitignore b/.gitignore index 85f31d1..5cba94c 100644 --- a/.gitignore +++ b/.gitignore @@ -1,5 +1,5 @@ .snakemake -ensembl -output +resources/ensembl +results input -ESCG_data \ No newline at end of file +**/.DS_Store diff --git a/.gitmodules b/.gitmodules new file mode 100644 index 0000000..1a0214b --- /dev/null +++ b/.gitmodules @@ -0,0 +1,3 @@ +[submodule "SingleCellProteogenomics"] + path = SingleCellProteogenomics + url = https://github.com/CellProfiling/SingleCellProteogenomics.git diff --git a/README.md b/README.md index a0d7fe9..c3fa6fe 100644 --- a/README.md +++ b/README.md @@ -6,19 +6,21 @@ This repository contains the _snakemake_ pipeline for analyzing the RNA sequenci ## Single-cell sequencing files -The single-cell RNA-Seq data is available at GEO SRA under project number [GSE146773](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE146773). +The single-cell RNA-Seq data is available at GEO SRA under project number [GSE146773](https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE146773). + +This data is downloaded automatically in this pipeline. ## Updating the Ensembl version -The genome and ensembl versions are located at the top of the file `Snakefile`. +The genome and Ensembl versions are located at the top of the file `Snakefile`. These can be updated, and the references will be downloaded automatically. ## Usage +1) Clone repository and initialize submodules: `git clone --recurse-submodules https://github.com/CellProfiling/FucciSingleCellSeqPipeline.git && cd FucciSingleCellSeqPipeline` 1) Install conda: https://docs.conda.io/en/latest/miniconda.html -2) Create the conda environment: `conda env create --file environment.yaml --name cellquant` -3) Activate the conda environment: `conda activate cellquant` -4) Run the workflow: recommended command is `snakemake --cores 24 --resources mem_mb=100000`, where you can subsitute the max number of cores and max memory allocation. The memory allocation should be at least 50000 MB if possible. It might work with 32000 MB, but no guarantees. +2) Install snakemake using conda: `conda install -c conda-forge snakemake-minimal` +4) Run the workflow: `snakemake --use-conda --cores 24 --resources mem_mb=100000`, where you can subsitute the max number of cores and max memory allocation. At least 54 GB of free memory should be available. ## Citation diff --git a/SingleCellProteogenomics b/SingleCellProteogenomics new file mode 160000 index 0000000..7f73b9a --- /dev/null +++ b/SingleCellProteogenomics @@ -0,0 +1 @@ +Subproject commit 7f73b9a17df5f7f17d944c756c10a70b0414199d diff --git a/Snakefile b/Snakefile deleted file mode 100644 index 1b713bf..0000000 --- a/Snakefile +++ /dev/null @@ -1,65 +0,0 @@ -GENOME_VERSION = "GRCh38" -ENSEMBL_VERSION = "96" -GENEMODEL_VERSION = GENOME_VERSION + "." + ENSEMBL_VERSION -GENOME_FA = f"ensembl/Homo_sapiens.{GENOME_VERSION}.dna.primary_assembly.fa" -ENSEMBL_GFF = f"ensembl/Homo_sapiens.{GENEMODEL_VERSION}.gff3" -TEST_GENOME_FA = f"ensembl/202122.fa" -TEST_ENSEMBL_GFF = f"ensembl/202122.gff3" -FA=GENOME_FA # for analysis; can also be TEST_GENOME_FA -GFF3=ENSEMBL_GFF # for analysis; can also be TEST_ENSEMBL_GFF -REFSTAR_PREFIX = f"ensembl/Homo_sapiens.{GENEMODEL_VERSION}RsemStar/RsemStarReference" -REFSTAR_FOLDER = f"ensembl/Homo_sapiens.{GENEMODEL_VERSION}RsemStar/" -REF_PREFIX = f"ensembl/Homo_sapiens.{GENEMODEL_VERSION}Rsem/RsemReference" -REF_FOLDER = f"ensembl/Homo_sapiens.{GENEMODEL_VERSION}Rsem/" -STAR_REF_FOLDER = f"ensembl/Homo_sapiens.{GENEMODEL_VERSION}Star" # no slash - -# FQ_FOLDER = "/mnt/e/ProjectsActive/tonsil/data_spritzsnake/trimmed/" -# configfile: "config.yaml" -FQ_FOLDER = "ESCG_data/" -(FQ_PREFIXES,) = glob_wildcards(FQ_FOLDER + "{fq}.fastq.gz") -# FQ_PREFIXES = config["fastq_prefixes"] - -def unique_tag(): - return str(len(str(expand("{fq}", fq=FQ_PREFIXES)))) - -rule all: - input: "output/Counts.csv", "output/Counts_Isoforms.csv" - -rule clean: - shell: "rm -rf ensembl output" - -include: "rules/quant.smk" - -rule download_ensembl_genome: - '''Download the genome with the version specified above''' - output: gfa=GENOME_FA, - log: "ensembl/downloads_fa.log" - shell: - "(wget -O - ftp://ftp.ensembl.org/pub/release-81//fasta/homo_sapiens/dna/Homo_sapiens." + GENOME_VERSION + ".dna.primary_assembly.fa.gz | " - "gunzip -c > {output.gfa}) 2> {log}" - -rule download_ensembl_genemodel: - '''Download the gene model with the version specified above''' - output: gff=ENSEMBL_GFF, - log: "ensembl/download_gff.log" - shell: - "(wget -O - ftp://ftp.ensembl.org/pub/release-" + ENSEMBL_VERSION + "/gff3/homo_sapiens/Homo_sapiens." + GENEMODEL_VERSION + ".gff3.gz | " - "gunzip -c > {output.gff}) 2> {log}" - -rule fix_gff3_for_rsem: - '''This script changes descriptive notes in column 4 to "gene" if a gene row, and it also adds ERCCs to the gene model''' - input: ENSEMBL_GFF - output: ENSEMBL_GFF + ".fix.gff3" - shell: "python scripts/fix_gff3_for_rsem.py {input} {output}" - -rule filter_gff3: - '''For testing, make a smaller gene model''' - input: ENSEMBL_GFF + ".fix.gff3" - output: TEST_ENSEMBL_GFF + ".fix.gff3" - shell: "grep \"^ERCC\\|^#\\|^20\\|^21\\|^22\" {input} > {output}" - -rule filter_fa: - '''For testing, make a smaller genome''' - input: GENOME_FA - output: TEST_GENOME_FA - script: "scripts/filter_fasta.py" diff --git a/environment.yaml b/environment.yaml deleted file mode 100644 index 011f281..0000000 --- a/environment.yaml +++ /dev/null @@ -1,16 +0,0 @@ -channels: - - bioconda - - conda-forge -dependencies: - - snakemake-minimal =5.2.4 - - python =3.7 - - rsem =1.3.2-0 - - samtools =1.9 - - git - - fastqc - - skewer - - star =2.7.0f-0 - - numpy - - biopython - - bcbio-gff - - pandas diff --git a/data/ERCC.fa b/resources/ERCC.fa similarity index 100% rename from data/ERCC.fa rename to resources/ERCC.fa diff --git a/resources/GSE146773_series_matrix.txt b/resources/GSE146773_series_matrix.txt new file mode 100644 index 0000000..b2c1f03 --- /dev/null +++ b/resources/GSE146773_series_matrix.txt @@ -0,0 +1,94 @@ +!Series_title "Single-cell temporal transcriptomics of the human cell cycle" +!Series_geo_accession "GSE146773" +!Series_status "Public on Dec 11 2020" +!Series_submission_date "Mar 11 2020" +!Series_last_update_date "Dec 13 2020" +!Series_summary "We present the first comprehensive map of spatiotemporal heterogeneity of the human proteome by integrating proteomics at subcellular resolution, single-cell transcriptomics, and pseudotime measurements of each cell within the cell cycle." +!Series_overall_design "A total of 1,152 single U2OS FUCCI cells were sorted by FACS, and RNA-Seq was collected for each cell using SMART-seq2 chemistry." +!Series_type "Expression profiling by high throughput sequencing" +!Series_contributor "Diana,,Mahdessian" +!Series_contributor "Anthony,J,Cesnik" +!Series_contributor "Christian,,Gnann" +!Series_contributor "Frida,,Danielsson" +!Series_contributor "Lovisa,,Stenström" +!Series_contributor "Muhammad,,Arif" +!Series_contributor "Cheng,,Zhang" +!Series_contributor "Trang,,Le" +!Series_contributor "Fredric,,Johansson" +!Series_contributor "Rutger,,Shutten" +!Series_contributor "Anna,,Bäckström" +!Series_contributor "Ulrika,,Axelsson" +!Series_contributor "Peter,,Thul" +!Series_contributor "Nathan,H,Cho" +!Series_contributor "Oana,,Carja" +!Series_contributor "Mathias,,Uhlén" +!Series_contributor "Adil,,Mardinoglu" +!Series_contributor "Charlotte,,Stadler" +!Series_contributor "Cecilia,,Lindskog" +!Series_contributor "Burcu,,Ayoglu" +!Series_contributor "Manuel,D,Leonetti" +!Series_contributor "Fredrik,,Pontén" +!Series_contributor "Devin,,Sullivan" +!Series_contributor "Emma,,Lundberg" +!Series_sample_id "GSM4405639 GSM4405640 GSM4405641 GSM4405642 GSM4405643 GSM4405644 GSM4405645 GSM4405646 GSM4405647 GSM4405648 GSM4405649 GSM4405650 GSM4405651 GSM4405652 GSM4405653 GSM4405654 GSM4405655 GSM4405656 GSM4405657 GSM4405658 GSM4405659 GSM4405660 GSM4405661 GSM4405662 GSM4405663 GSM4405664 GSM4405665 GSM4405666 GSM4405667 GSM4405668 GSM4405669 GSM4405670 GSM4405671 GSM4405672 GSM4405673 GSM4405674 GSM4405675 GSM4405676 GSM4405677 GSM4405678 GSM4405679 GSM4405680 GSM4405681 GSM4405682 GSM4405683 GSM4405684 GSM4405685 GSM4405686 GSM4405687 GSM4405688 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GSM4406508 GSM4406509 GSM4406510 GSM4406511 GSM4406512 GSM4406513 GSM4406514 GSM4406515 GSM4406516 GSM4406517 GSM4406518 GSM4406519 GSM4406520 GSM4406521 GSM4406522 GSM4406523 GSM4406524 GSM4406525 GSM4406526 GSM4406527 GSM4406528 GSM4406529 GSM4406530 GSM4406531 GSM4406532 GSM4406533 GSM4406534 GSM4406535 GSM4406536 GSM4406537 GSM4406538 GSM4406539 GSM4406540 GSM4406541 GSM4406542 GSM4406543 GSM4406544 GSM4406545 GSM4406546 GSM4406547 GSM4406548 GSM4406549 GSM4406550 GSM4406551 GSM4406552 GSM4406553 GSM4406554 GSM4406555 GSM4406556 GSM4406557 GSM4406558 GSM4406559 GSM4406560 GSM4406561 GSM4406562 GSM4406563 GSM4406564 GSM4406565 GSM4406566 GSM4406567 GSM4406568 GSM4406569 GSM4406570 GSM4406571 GSM4406572 GSM4406573 GSM4406574 GSM4406575 GSM4406576 GSM4406577 GSM4406578 GSM4406579 GSM4406580 GSM4406581 GSM4406582 GSM4406583 GSM4406584 GSM4406585 GSM4406586 GSM4406587 GSM4406588 GSM4406589 GSM4406590 GSM4406591 GSM4406592 GSM4406593 GSM4406594 GSM4406595 GSM4406596 GSM4406597 GSM4406598 GSM4406599 GSM4406600 GSM4406601 GSM4406602 GSM4406603 GSM4406604 GSM4406605 GSM4406606 GSM4406607 GSM4406608 GSM4406609 GSM4406610 GSM4406611 GSM4406612 GSM4406613 GSM4406614 GSM4406615 GSM4406616 GSM4406617 GSM4406618 GSM4406619 GSM4406620 GSM4406621 GSM4406622 GSM4406623 GSM4406624 GSM4406625 GSM4406626 GSM4406627 GSM4406628 GSM4406629 GSM4406630 GSM4406631 GSM4406632 GSM4406633 GSM4406634 GSM4406635 GSM4406636 GSM4406637 GSM4406638 GSM4406639 GSM4406640 GSM4406641 GSM4406642 GSM4406643 GSM4406644 GSM4406645 GSM4406646 GSM4406647 GSM4406648 GSM4406649 GSM4406650 GSM4406651 GSM4406652 GSM4406653 GSM4406654 GSM4406655 GSM4406656 GSM4406657 GSM4406658 GSM4406659 GSM4406660 GSM4406661 GSM4406662 GSM4406663 GSM4406664 GSM4406665 GSM4406666 GSM4406667 GSM4406668 GSM4406669 GSM4406670 GSM4406671 GSM4406672 GSM4406673 GSM4406674 GSM4406675 GSM4406676 GSM4406677 GSM4406678 GSM4406679 GSM4406680 GSM4406681 GSM4406682 GSM4406683 GSM4406684 GSM4406685 GSM4406686 GSM4406687 GSM4406688 GSM4406689 GSM4406690 GSM4406691 GSM4406692 GSM4406693 GSM4406694 GSM4406695 GSM4406696 GSM4406697 GSM4406698 GSM4406699 GSM4406700 GSM4406701 GSM4406702 GSM4406703 GSM4406704 GSM4406705 GSM4406706 GSM4406707 GSM4406708 GSM4406709 GSM4406710 GSM4406711 GSM4406712 GSM4406713 GSM4406714 GSM4406715 GSM4406716 GSM4406717 GSM4406718 GSM4406719 GSM4406720 GSM4406721 GSM4406722 GSM4406723 GSM4406724 GSM4406725 GSM4406726 GSM4406727 GSM4406728 GSM4406729 GSM4406730 GSM4406731 GSM4406732 GSM4406733 GSM4406734 GSM4406735 GSM4406736 GSM4406737 GSM4406738 GSM4406739 GSM4406740 GSM4406741 GSM4406742 GSM4406743 GSM4406744 GSM4406745 GSM4406746 GSM4406747 GSM4406748 GSM4406749 GSM4406750 GSM4406751 GSM4406752 GSM4406753 GSM4406754 GSM4406755 GSM4406756 GSM4406757 GSM4406758 GSM4406759 GSM4406760 GSM4406761 GSM4406762 GSM4406763 GSM4406764 GSM4406765 GSM4406766 GSM4406767 GSM4406768 GSM4406769 GSM4406770 GSM4406771 GSM4406772 GSM4406773 GSM4406774 GSM4406775 GSM4406776 GSM4406777 GSM4406778 GSM4406779 GSM4406780 GSM4406781 GSM4406782 GSM4406783 GSM4406784 GSM4406785 GSM4406786 GSM4406787 GSM4406788 GSM4406789 GSM4406790 " +!Series_contact_name "Emma,,Lundberg" +!Series_contact_email "emma.lundberg@scilifelab.se" +!Series_contact_phone "+4670333363" +!Series_contact_laboratory "Lundberg" +!Series_contact_institute "KTH Royal Institute of Technology" +!Series_contact_address "Science for Life Laboratory, Tomtebodavägen 23A" +!Series_contact_city "Solna" +!Series_contact_zip/postal_code "17121" +!Series_contact_country "Sweden" +!Series_supplementary_file "ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE146nnn/GSE146773/suppl/GSE146773_Counts.csv.ercc.csv.gz" +!Series_supplementary_file "ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE146nnn/GSE146773/suppl/GSE146773_Counts.csv.gz" +!Series_supplementary_file "ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE146nnn/GSE146773/suppl/GSE146773_RAW.tar" +!Series_supplementary_file "ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE146nnn/GSE146773/suppl/GSE146773_Tpms.csv.ercc.csv.gz" +!Series_supplementary_file "ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE146nnn/GSE146773/suppl/GSE146773_Tpms.csv.gz" +!Series_citation "https://doi.org/10.1101/543231" +!Series_platform_id "GPL11154" +!Series_platform_taxid "9606" +!Series_sample_taxid "9606" +!Series_relation "BioProject: https://www.ncbi.nlm.nih.gov/bioproject/PRJNA611937" +!Series_relation "SRA: https://www.ncbi.nlm.nih.gov/sra?term=SRP252355" + +!Sample_title "Single U2OS cell A10_355" "Single U2OS cell A10_356" "Single U2OS cell A10_357" "Single U2OS cell A11_355" "Single U2OS cell A11_356" "Single U2OS cell A11_357" "Single U2OS cell A12_355" "Single U2OS cell A12_356" "Single U2OS cell A12_357" "Single U2OS cell A13_355" "Single U2OS cell A13_356" "Single U2OS cell A13_357" "Single U2OS cell A14_355" "Single U2OS cell A14_356" "Single U2OS cell A14_357" "Single U2OS cell A15_355" "Single U2OS cell A15_356" "Single U2OS cell A15_357" "Single U2OS cell A16_355" "Single U2OS cell A16_356" "Single U2OS cell A16_357" "Single U2OS cell A17_355" "Single U2OS cell A17_356" "Single U2OS cell A17_357" "Single U2OS cell A18_355" "Single U2OS cell A18_356" "Single U2OS cell A18_357" "Single U2OS cell A19_355" "Single U2OS cell A19_356" "Single U2OS cell A19_357" "Single U2OS cell A1_355" "Single U2OS cell A1_356" "Single U2OS cell A1_357" "Single U2OS cell A20_355" "Single U2OS cell A20_356" "Single U2OS cell A20_357" "Single U2OS cell A21_355" "Single U2OS cell A21_356" "Single U2OS cell A21_357" "Single U2OS cell A22_355" "Single U2OS cell A22_356" "Single U2OS cell A22_357" "Single U2OS cell A23_355" "Single U2OS cell A23_356" "Single U2OS cell A23_357" "Single U2OS cell A24_355" "Single U2OS cell A24_356" "Single U2OS cell A24_357" "Single U2OS cell A2_355" "Single U2OS cell A2_356" "Single U2OS cell A2_357" "Single U2OS cell A3_355" "Single U2OS cell A3_356" "Single U2OS cell A3_357" "Single U2OS cell A4_355" "Single U2OS cell A4_356" "Single U2OS cell A4_357" "Single U2OS cell A5_355" "Single U2OS cell A5_356" "Single U2OS cell A5_357" "Single U2OS cell A6_355" "Single U2OS cell A6_356" "Single U2OS cell A6_357" "Single U2OS cell A7_355" "Single U2OS cell A7_356" "Single U2OS cell A7_357" "Single U2OS cell A8_355" "Single U2OS cell A8_356" "Single U2OS cell A8_357" "Single U2OS cell A9_355" "Single U2OS cell A9_356" "Single U2OS cell A9_357" "Single U2OS cell B10_355" "Single U2OS cell B10_356" "Single U2OS cell B10_357" "Single U2OS cell B11_355" "Single U2OS cell B11_356" "Single U2OS cell B11_357" "Single U2OS cell B12_355" "Single U2OS cell B12_356" "Single U2OS cell B12_357" "Single U2OS cell B13_355" "Single U2OS cell B13_356" "Single U2OS cell B13_357" "Single U2OS cell B14_355" "Single U2OS cell B14_356" "Single U2OS cell B14_357" "Single U2OS cell B15_355" "Single U2OS cell B15_356" "Single U2OS cell B15_357" "Single U2OS cell B16_355" "Single U2OS cell B16_356" "Single U2OS cell B16_357" "Single U2OS cell B17_355" "Single U2OS cell B17_356" "Single U2OS cell B17_357" "Single U2OS cell B18_355" "Single U2OS cell B18_356" "Single U2OS cell B18_357" "Single U2OS cell B19_355" "Single U2OS cell B19_356" "Single U2OS cell B19_357" "Single U2OS cell B1_355" "Single U2OS cell B1_356" "Single U2OS cell B1_357" "Single U2OS cell B20_355" "Single U2OS cell B20_356" "Single U2OS cell B20_357" "Single U2OS cell B21_355" "Single U2OS cell B21_356" "Single U2OS cell B21_357" "Single U2OS cell B22_355" "Single U2OS cell B22_356" "Single U2OS cell B22_357" "Single U2OS cell B23_355" "Single U2OS cell B23_356" "Single U2OS cell B23_357" "Single U2OS cell B24_355" "Single U2OS cell B24_356" "Single U2OS cell B24_357" "Single U2OS cell B2_355" "Single U2OS cell B2_356" "Single U2OS cell B2_357" "Single U2OS cell B3_355" "Single U2OS cell B3_356" "Single U2OS cell B3_357" "Single U2OS cell B4_355" "Single U2OS cell B4_356" "Single U2OS cell B4_357" "Single U2OS cell B5_355" "Single U2OS cell B5_356" "Single U2OS cell B5_357" "Single U2OS cell B6_355" "Single U2OS cell B6_356" "Single U2OS cell B6_357" "Single U2OS cell B7_355" "Single U2OS cell B7_356" "Single U2OS cell B7_357" "Single U2OS cell B8_355" "Single U2OS cell B8_356" "Single U2OS cell B8_357" "Single U2OS cell B9_355" "Single U2OS cell B9_356" "Single U2OS cell B9_357" "Single U2OS cell C10_355" "Single U2OS cell C10_356" "Single U2OS cell C10_357" "Single U2OS cell C11_355" "Single U2OS cell C11_356" "Single U2OS cell C11_357" "Single U2OS cell C12_355" "Single U2OS cell C12_356" "Single U2OS cell C12_357" "Single U2OS cell C13_355" "Single U2OS cell C13_356" "Single U2OS cell C13_357" "Single U2OS cell C14_355" "Single U2OS cell C14_356" "Single U2OS cell C14_357" "Single U2OS cell C15_355" "Single U2OS cell C15_356" "Single U2OS cell C15_357" "Single U2OS cell C16_355" "Single U2OS cell C16_356" "Single U2OS cell C16_357" "Single U2OS cell C17_355" "Single U2OS cell C17_356" "Single U2OS cell C17_357" "Single U2OS cell C18_355" "Single U2OS cell C18_356" "Single U2OS cell C18_357" "Single U2OS cell C19_355" "Single U2OS cell C19_356" "Single U2OS cell C19_357" "Single U2OS cell C1_355" "Single U2OS cell C1_356" "Single U2OS cell C1_357" "Single U2OS cell C20_355" "Single U2OS cell C20_356" "Single U2OS cell C20_357" "Single U2OS cell C21_355" "Single U2OS cell C21_356" "Single U2OS cell C21_357" "Single U2OS cell C22_355" "Single U2OS cell C22_356" "Single U2OS cell C22_357" "Single U2OS cell C23_355" "Single U2OS cell C23_356" "Single U2OS cell C23_357" "Single U2OS cell C24_355" "Single U2OS cell C24_356" "Single U2OS cell C24_357" "Single U2OS cell C2_355" "Single U2OS cell C2_356" "Single U2OS cell C2_357" "Single U2OS cell C3_355" "Single U2OS cell C3_356" "Single U2OS cell C3_357" "Single U2OS cell C4_355" "Single U2OS cell C4_356" "Single U2OS cell C4_357" "Single U2OS cell C5_355" "Single U2OS cell C5_356" "Single U2OS cell C5_357" "Single U2OS cell C6_355" "Single U2OS cell C6_356" "Single U2OS cell C6_357" "Single U2OS cell C7_355" "Single U2OS cell C7_356" "Single U2OS cell C7_357" "Single U2OS cell C8_355" "Single U2OS cell C8_356" "Single U2OS cell C8_357" "Single U2OS cell C9_355" "Single U2OS cell C9_356" "Single U2OS cell C9_357" "Single U2OS cell D10_355" "Single U2OS cell D10_356" "Single U2OS cell D10_357" "Single U2OS cell D11_355" "Single U2OS cell D11_356" "Single U2OS cell D11_357" "Single U2OS cell D12_355" "Single U2OS cell D12_356" "Single U2OS cell D12_357" "Single U2OS cell D13_355" "Single U2OS cell D13_356" "Single U2OS cell D13_357" "Single U2OS cell D14_355" "Single U2OS cell D14_356" "Single U2OS cell D14_357" "Single U2OS cell D15_355" "Single U2OS cell D15_356" "Single U2OS cell D15_357" "Single U2OS cell D16_355" "Single U2OS cell D16_356" "Single U2OS cell D16_357" "Single U2OS cell D17_355" "Single U2OS cell D17_356" "Single U2OS cell D17_357" "Single U2OS cell D18_355" "Single U2OS cell D18_356" "Single U2OS cell D18_357" "Single U2OS cell D19_355" "Single U2OS cell D19_356" "Single U2OS cell D19_357" "Single U2OS cell D1_355" "Single U2OS cell D1_356" "Single U2OS cell D1_357" "Single U2OS cell D20_355" "Single U2OS cell D20_356" "Single U2OS cell D20_357" "Single U2OS cell D21_355" "Single U2OS cell D21_356" "Single U2OS cell D21_357" "Single U2OS cell D22_355" "Single U2OS cell D22_356" "Single U2OS cell D22_357" "Single U2OS cell D23_355" "Single U2OS cell D23_356" "Single U2OS cell D23_357" "Single U2OS cell D24_355" "Single U2OS cell D24_356" "Single U2OS cell D24_357" "Single U2OS cell D2_355" "Single U2OS cell D2_356" "Single U2OS cell D2_357" "Single U2OS cell D3_355" "Single U2OS cell D3_356" "Single U2OS cell D3_357" "Single U2OS cell D4_355" "Single U2OS cell D4_356" "Single U2OS cell D4_357" "Single U2OS cell D5_355" "Single U2OS cell D5_356" "Single U2OS cell D5_357" "Single U2OS cell D6_355" "Single U2OS cell D6_356" "Single U2OS cell D6_357" "Single U2OS cell D7_355" "Single U2OS cell D7_356" "Single U2OS cell D7_357" "Single U2OS cell D8_355" "Single U2OS cell D8_356" "Single U2OS cell D8_357" "Single U2OS cell D9_355" "Single U2OS cell D9_356" "Single U2OS cell D9_357" "Single U2OS cell E10_355" "Single U2OS cell E10_356" "Single U2OS cell E10_357" "Single U2OS cell E11_355" "Single U2OS cell E11_356" "Single U2OS cell E11_357" "Single U2OS cell E12_355" "Single U2OS cell E12_356" "Single U2OS cell E12_357" "Single U2OS cell E13_355" "Single U2OS cell E13_356" "Single U2OS cell E13_357" "Single U2OS cell E14_355" "Single U2OS cell E14_356" "Single U2OS cell E14_357" "Single U2OS cell E15_355" "Single U2OS cell E15_356" "Single U2OS cell E15_357" "Single U2OS cell E16_355" "Single U2OS cell E16_356" "Single U2OS cell E16_357" "Single U2OS cell E17_355" "Single U2OS cell E17_356" "Single U2OS cell E17_357" "Single U2OS cell E18_355" "Single U2OS cell E18_356" "Single U2OS cell E18_357" "Single U2OS cell E19_355" "Single U2OS cell E19_356" "Single U2OS cell E19_357" "Single U2OS cell E1_355" "Single U2OS cell E1_356" "Single U2OS cell E1_357" "Single U2OS cell E20_355" "Single U2OS cell E20_356" "Single U2OS cell E20_357" "Single U2OS cell E21_355" "Single U2OS cell E21_356" "Single U2OS cell E21_357" "Single U2OS cell E22_355" "Single U2OS cell E22_356" "Single U2OS cell E22_357" "Single U2OS cell E23_355" "Single U2OS cell E23_356" "Single U2OS cell E23_357" "Single U2OS cell E24_355" "Single U2OS cell E24_356" "Single U2OS cell E24_357" "Single U2OS cell E2_355" "Single U2OS cell E2_356" "Single U2OS cell E2_357" "Single U2OS cell E3_355" "Single U2OS cell E3_356" "Single U2OS cell E3_357" "Single U2OS cell E4_355" "Single U2OS cell E4_356" "Single U2OS cell E4_357" "Single U2OS cell E5_355" "Single U2OS cell E5_356" "Single U2OS cell E5_357" "Single U2OS cell E6_355" "Single U2OS cell E6_356" "Single U2OS cell E6_357" "Single U2OS cell E7_355" "Single U2OS cell E7_356" "Single U2OS cell E7_357" "Single U2OS cell E8_355" "Single U2OS cell E8_356" "Single U2OS cell E8_357" "Single U2OS cell E9_355" "Single U2OS cell E9_356" "Single U2OS cell E9_357" "Single U2OS cell F10_355" "Single U2OS cell F10_356" "Single U2OS cell F10_357" "Single U2OS cell F11_355" "Single U2OS cell F11_356" "Single U2OS cell F11_357" "Single U2OS cell F12_355" "Single U2OS cell F12_356" "Single U2OS cell F12_357" "Single U2OS cell F13_355" "Single U2OS cell F13_356" "Single U2OS cell F13_357" "Single U2OS cell F14_355" "Single U2OS cell F14_356" "Single U2OS cell F14_357" "Single U2OS cell F15_355" "Single U2OS cell F15_356" "Single U2OS cell F15_357" "Single U2OS cell F16_355" "Single U2OS cell F16_356" "Single U2OS cell F16_357" "Single U2OS cell F17_355" "Single U2OS cell F17_356" "Single U2OS cell F17_357" "Single U2OS cell F18_355" "Single U2OS cell F18_356" "Single U2OS cell F18_357" "Single U2OS cell F19_355" "Single U2OS cell F19_356" "Single U2OS cell F19_357" "Single U2OS cell F1_355" "Single U2OS cell F1_356" "Single U2OS cell F1_357" "Single U2OS cell F20_355" "Single U2OS cell F20_356" "Single U2OS cell F20_357" "Single U2OS cell F21_355" "Single U2OS cell F21_356" "Single U2OS cell F21_357" "Single U2OS cell F22_355" "Single U2OS cell F22_356" "Single U2OS cell F22_357" "Single U2OS cell F23_355" "Single U2OS cell F23_356" "Single U2OS cell F23_357" "Single U2OS cell F24_355" "Single U2OS cell F24_356" "Single U2OS cell F24_357" "Single U2OS cell F2_355" "Single U2OS cell F2_356" "Single U2OS cell F2_357" "Single U2OS cell F3_355" "Single U2OS cell F3_356" "Single U2OS cell F3_357" "Single U2OS cell F4_355" "Single U2OS cell F4_356" "Single U2OS cell F4_357" "Single U2OS cell F5_355" "Single U2OS cell F5_356" "Single U2OS cell F5_357" "Single U2OS cell F6_355" "Single U2OS cell F6_356" "Single U2OS cell F6_357" "Single U2OS cell F7_355" "Single U2OS cell F7_356" "Single U2OS cell F7_357" "Single U2OS cell F8_355" "Single U2OS cell F8_356" "Single U2OS cell F8_357" "Single U2OS cell F9_355" "Single U2OS cell F9_356" "Single U2OS cell F9_357" "Single U2OS cell G10_355" "Single U2OS cell G10_356" "Single U2OS cell G10_357" "Single U2OS cell G11_355" "Single U2OS cell G11_356" "Single U2OS cell G11_357" "Single U2OS cell G12_355" "Single U2OS cell G12_356" "Single U2OS cell G12_357" "Single U2OS cell G13_355" "Single U2OS cell G13_356" "Single U2OS cell G13_357" "Single U2OS cell G14_355" "Single U2OS cell G14_356" "Single U2OS cell G14_357" "Single U2OS cell G15_355" "Single U2OS cell G15_356" "Single U2OS cell G15_357" "Single U2OS cell G16_355" "Single U2OS cell G16_356" "Single U2OS cell G16_357" "Single U2OS cell G17_355" "Single U2OS cell G17_356" "Single U2OS cell G17_357" "Single U2OS cell G18_355" "Single U2OS cell G18_356" "Single U2OS cell G18_357" "Single U2OS cell G19_355" "Single U2OS cell G19_356" "Single U2OS cell G19_357" "Single U2OS cell G1_355" "Single U2OS cell G1_356" "Single U2OS cell G1_357" "Single U2OS cell G20_355" "Single U2OS cell G20_356" "Single U2OS cell G20_357" "Single U2OS cell G21_355" "Single U2OS cell G21_356" "Single U2OS cell G21_357" "Single U2OS cell G22_355" "Single U2OS cell G22_356" "Single U2OS cell G22_357" "Single U2OS cell G23_355" "Single U2OS cell G23_356" "Single U2OS cell G23_357" "Single U2OS cell G24_355" "Single U2OS cell G24_356" "Single U2OS cell G24_357" "Single U2OS cell G2_355" "Single U2OS cell G2_356" "Single U2OS cell G2_357" "Single U2OS cell G3_355" "Single U2OS cell G3_356" "Single U2OS cell G3_357" "Single U2OS cell G4_355" "Single U2OS cell G4_356" "Single U2OS cell G4_357" "Single U2OS cell G5_355" "Single U2OS cell G5_356" "Single U2OS cell G5_357" "Single U2OS cell G6_355" "Single U2OS cell G6_356" "Single U2OS cell G6_357" "Single U2OS cell G7_355" "Single U2OS cell G7_356" "Single U2OS cell G7_357" "Single U2OS cell G8_355" "Single U2OS cell G8_356" "Single U2OS cell G8_357" "Single U2OS cell G9_355" "Single U2OS cell G9_356" "Single U2OS cell G9_357" "Single U2OS cell H10_355" "Single U2OS cell H10_356" "Single U2OS cell H10_357" "Single U2OS cell H11_355" "Single U2OS cell H11_356" "Single U2OS cell H11_357" "Single U2OS cell H12_355" "Single U2OS cell H12_356" "Single U2OS cell H12_357" "Single U2OS cell H13_355" "Single U2OS cell H13_356" "Single U2OS cell H13_357" "Single U2OS cell H14_355" "Single U2OS cell H14_356" "Single U2OS cell H14_357" "Single U2OS cell H15_355" "Single U2OS cell H15_356" "Single U2OS cell H15_357" "Single U2OS cell H16_355" "Single U2OS cell H16_356" "Single U2OS cell H16_357" "Single U2OS cell H17_355" "Single U2OS cell H17_356" "Single U2OS cell H17_357" "Single U2OS cell H18_355" "Single U2OS cell H18_356" "Single U2OS cell H18_357" "Single U2OS cell H19_355" "Single U2OS cell H19_356" "Single U2OS cell H19_357" "Single U2OS cell H1_355" "Single U2OS cell H1_356" "Single U2OS cell H1_357" "Single U2OS cell H20_355" "Single U2OS cell H20_356" "Single U2OS cell H20_357" "Single U2OS cell H21_355" "Single U2OS cell H21_356" "Single U2OS cell H21_357" "Single U2OS cell H22_355" "Single U2OS cell H22_356" "Single U2OS cell H22_357" "Single U2OS cell H23_355" "Single U2OS cell H23_356" "Single U2OS cell H23_357" "Single U2OS cell H24_355" "Single U2OS cell H24_356" "Single U2OS cell H24_357" "Single U2OS cell H2_355" "Single U2OS cell H2_356" "Single U2OS cell H2_357" "Single U2OS cell H3_355" "Single U2OS cell H3_356" "Single U2OS cell H3_357" "Single U2OS cell H4_355" "Single U2OS cell H4_356" "Single U2OS cell H4_357" "Single U2OS cell H5_355" "Single U2OS cell H5_356" "Single U2OS cell H5_357" "Single U2OS cell H6_355" "Single U2OS cell H6_356" "Single U2OS cell H6_357" "Single U2OS cell H7_355" "Single U2OS cell H7_356" "Single U2OS cell H7_357" "Single U2OS cell H8_355" "Single U2OS cell H8_356" "Single U2OS cell H8_357" "Single U2OS cell H9_355" "Single U2OS cell H9_356" "Single U2OS cell H9_357" "Single U2OS cell I10_355" "Single U2OS cell I10_356" "Single U2OS cell I10_357" "Single U2OS cell I11_355" "Single U2OS cell I11_356" "Single U2OS cell I11_357" "Single U2OS cell I12_355" "Single U2OS cell I12_356" "Single U2OS cell I12_357" "Single U2OS cell I13_355" "Single U2OS cell I13_356" "Single U2OS cell I13_357" "Single U2OS cell I14_355" "Single U2OS cell I14_356" "Single U2OS cell I14_357" "Single U2OS cell I15_355" "Single U2OS cell I15_356" "Single U2OS cell I15_357" "Single U2OS cell I16_355" "Single U2OS cell I16_356" "Single U2OS cell I16_357" "Single U2OS cell I17_355" "Single U2OS cell I17_356" "Single U2OS cell I17_357" "Single U2OS cell I18_355" "Single U2OS cell I18_356" "Single U2OS cell I18_357" "Single U2OS cell I19_355" "Single U2OS cell I19_356" "Single U2OS cell I19_357" "Single U2OS cell I1_355" "Single U2OS cell I1_356" "Single U2OS cell I1_357" "Single U2OS cell I20_355" "Single U2OS cell I20_356" "Single U2OS cell I20_357" "Single U2OS cell I21_355" "Single U2OS cell I21_356" "Single U2OS cell I21_357" "Single U2OS cell I22_355" "Single U2OS cell I22_356" "Single U2OS cell I22_357" "Single U2OS cell I23_355" "Single U2OS cell I23_356" "Single U2OS cell I23_357" "Single U2OS cell I24_355" "Single U2OS cell I24_356" "Single U2OS cell I24_357" "Single U2OS cell I2_355" "Single U2OS cell I2_356" "Single U2OS cell I2_357" "Single U2OS cell I3_355" "Single U2OS cell I3_356" "Single U2OS cell I3_357" "Single U2OS cell I4_355" "Single U2OS cell I4_356" "Single U2OS cell I4_357" "Single U2OS cell I5_355" "Single U2OS cell I5_356" "Single U2OS cell I5_357" "Single U2OS cell I6_355" "Single U2OS cell I6_356" "Single U2OS cell I6_357" "Single U2OS cell I7_355" "Single U2OS cell I7_356" "Single U2OS cell I7_357" "Single U2OS cell I8_355" "Single U2OS cell I8_356" "Single U2OS cell I8_357" "Single U2OS cell I9_355" "Single U2OS cell I9_356" "Single U2OS cell I9_357" "Single U2OS cell J10_355" "Single U2OS cell J10_356" "Single U2OS cell J10_357" "Single U2OS cell J11_355" "Single U2OS cell J11_356" "Single U2OS cell J11_357" "Single U2OS cell J12_355" "Single U2OS cell J12_356" "Single U2OS cell J12_357" "Single U2OS cell J13_355" "Single U2OS cell J13_356" "Single U2OS cell J13_357" "Single U2OS cell J14_355" "Single U2OS cell J14_356" "Single U2OS cell J14_357" "Single U2OS cell J15_355" "Single U2OS cell J15_356" "Single U2OS cell J15_357" "Single U2OS cell J16_355" "Single U2OS cell J16_356" "Single U2OS cell J16_357" "Single U2OS cell J17_355" "Single U2OS cell J17_356" "Single U2OS cell J17_357" "Single U2OS cell J18_355" "Single U2OS cell J18_356" "Single U2OS cell J18_357" "Single U2OS cell J19_355" "Single U2OS cell J19_356" "Single U2OS cell J19_357" "Single U2OS cell J1_355" "Single U2OS cell J1_356" "Single U2OS cell J1_357" "Single U2OS cell J20_355" "Single U2OS cell J20_356" "Single U2OS cell J20_357" "Single U2OS cell J21_355" "Single U2OS cell J21_356" "Single U2OS cell J21_357" "Single U2OS cell J22_355" "Single U2OS cell J22_356" "Single U2OS cell J22_357" "Single U2OS cell J23_355" "Single U2OS cell J23_356" "Single U2OS cell J23_357" "Single U2OS cell J24_355" "Single U2OS cell J24_356" "Single U2OS cell J24_357" "Single U2OS cell J2_355" "Single U2OS cell J2_356" "Single U2OS cell J2_357" "Single U2OS cell J3_355" "Single U2OS cell J3_356" "Single U2OS cell J3_357" "Single U2OS cell J4_355" "Single U2OS cell J4_356" "Single U2OS cell J4_357" "Single U2OS cell J5_355" "Single U2OS cell J5_356" "Single U2OS cell J5_357" "Single U2OS cell J6_355" "Single U2OS cell J6_356" "Single U2OS cell J6_357" "Single U2OS cell J7_355" "Single U2OS cell J7_356" "Single U2OS cell J7_357" "Single U2OS cell J8_355" "Single U2OS cell J8_356" "Single U2OS cell J8_357" "Single U2OS cell J9_355" "Single U2OS cell J9_356" "Single U2OS cell J9_357" "Single U2OS cell K10_355" "Single U2OS cell K10_356" "Single U2OS cell K10_357" "Single U2OS cell K11_355" "Single U2OS cell K11_356" "Single U2OS cell K11_357" "Single U2OS cell K12_355" "Single U2OS cell K12_356" "Single U2OS cell K12_357" "Single U2OS cell K13_355" "Single U2OS cell K13_356" "Single U2OS cell K13_357" "Single U2OS cell K14_355" "Single U2OS cell K14_356" "Single U2OS cell K14_357" "Single U2OS cell K15_355" "Single U2OS cell K15_356" "Single U2OS cell K15_357" "Single U2OS cell K16_355" "Single U2OS cell K16_356" "Single U2OS cell K16_357" "Single U2OS cell K17_355" "Single U2OS cell K17_356" "Single U2OS cell K17_357" "Single U2OS cell K18_355" "Single U2OS cell K18_356" "Single U2OS cell K18_357" "Single U2OS cell K19_355" "Single U2OS cell K19_356" "Single U2OS cell K19_357" "Single U2OS cell K1_355" "Single U2OS cell K1_356" "Single U2OS cell K1_357" "Single U2OS cell K20_355" "Single U2OS cell K20_356" "Single U2OS cell K20_357" "Single U2OS cell K21_355" "Single U2OS cell K21_356" "Single U2OS cell K21_357" "Single U2OS cell K22_355" "Single U2OS cell K22_356" "Single U2OS cell K22_357" "Single U2OS cell K23_355" "Single U2OS cell K23_356" "Single U2OS cell K23_357" "Single U2OS cell K24_355" "Single U2OS cell K24_356" "Single U2OS cell K24_357" "Single U2OS cell K2_355" "Single U2OS cell K2_356" "Single U2OS cell K2_357" "Single U2OS cell K3_355" "Single U2OS cell K3_356" "Single U2OS cell K3_357" "Single U2OS cell K4_355" "Single U2OS cell K4_356" "Single U2OS cell K4_357" "Single U2OS cell K5_355" "Single U2OS cell K5_356" "Single U2OS cell K5_357" "Single U2OS cell K6_355" "Single U2OS cell K6_356" "Single U2OS cell K6_357" "Single U2OS cell K7_355" "Single U2OS cell K7_356" "Single U2OS cell K7_357" "Single U2OS cell K8_355" "Single U2OS cell K8_356" "Single U2OS cell K8_357" "Single U2OS cell K9_355" "Single U2OS cell K9_356" "Single U2OS cell K9_357" "Single U2OS cell L10_355" "Single U2OS cell L10_356" "Single U2OS cell L10_357" "Single U2OS cell L11_355" "Single U2OS cell L11_356" "Single U2OS cell L11_357" "Single U2OS cell L12_355" "Single U2OS cell L12_356" "Single U2OS cell L12_357" "Single U2OS cell L13_355" "Single U2OS cell L13_356" "Single U2OS cell L13_357" "Single U2OS cell L14_355" "Single U2OS cell L14_356" "Single U2OS cell L14_357" "Single U2OS cell L15_355" "Single U2OS cell L15_356" "Single U2OS cell L15_357" "Single U2OS cell L16_355" "Single U2OS cell L16_356" "Single U2OS cell L16_357" "Single U2OS cell L17_355" "Single U2OS cell L17_356" "Single U2OS cell L17_357" "Single U2OS cell L18_355" "Single U2OS cell L18_356" "Single U2OS cell L18_357" "Single U2OS cell L19_355" "Single U2OS cell L19_356" "Single U2OS cell L19_357" "Single U2OS cell L1_355" "Single U2OS cell L1_356" "Single U2OS cell L1_357" "Single U2OS cell L20_355" "Single U2OS cell L20_356" "Single U2OS cell L20_357" "Single U2OS cell L21_355" "Single U2OS cell L21_356" "Single U2OS cell L21_357" "Single U2OS cell L22_355" "Single U2OS cell L22_356" "Single U2OS cell L22_357" "Single U2OS cell L23_355" "Single U2OS cell L23_356" "Single U2OS cell L23_357" "Single U2OS cell L24_355" "Single U2OS cell L24_356" "Single U2OS cell L24_357" "Single U2OS cell L2_355" "Single U2OS cell L2_356" "Single U2OS cell L2_357" "Single U2OS cell L3_355" "Single U2OS cell L3_356" "Single U2OS cell L3_357" "Single U2OS cell L4_355" "Single U2OS cell L4_356" "Single U2OS cell L4_357" "Single U2OS cell L5_355" "Single U2OS cell L5_356" "Single U2OS cell L5_357" "Single U2OS cell L6_355" "Single U2OS cell L6_356" "Single U2OS cell L6_357" "Single U2OS cell L7_355" "Single U2OS cell L7_356" "Single U2OS cell L7_357" "Single U2OS cell L8_355" "Single U2OS cell L8_356" "Single U2OS cell L8_357" "Single U2OS cell L9_355" "Single U2OS cell L9_356" "Single U2OS cell L9_357" "Single U2OS cell M10_355" "Single U2OS cell M10_356" "Single U2OS cell M10_357" "Single U2OS cell M11_355" "Single U2OS cell M11_356" "Single U2OS cell M11_357" "Single U2OS cell M12_355" "Single U2OS cell M12_356" "Single U2OS cell M12_357" "Single U2OS cell M13_355" "Single U2OS cell M13_356" "Single U2OS cell M13_357" "Single U2OS cell M14_355" "Single U2OS cell M14_356" "Single U2OS cell M14_357" "Single U2OS cell M15_355" "Single U2OS cell M15_356" "Single U2OS cell M15_357" "Single U2OS cell M16_355" "Single U2OS cell M16_356" "Single U2OS cell M16_357" "Single U2OS cell M17_355" "Single U2OS cell M17_356" "Single U2OS cell M17_357" "Single U2OS cell M18_355" "Single U2OS cell M18_356" "Single U2OS cell M18_357" "Single U2OS cell M19_355" "Single U2OS cell M19_356" "Single U2OS cell M19_357" "Single U2OS cell M1_355" "Single U2OS cell M1_356" "Single U2OS cell M1_357" "Single U2OS cell M20_355" "Single U2OS cell M20_356" "Single U2OS cell M20_357" "Single U2OS cell M21_355" "Single U2OS cell M21_356" "Single U2OS cell M21_357" "Single U2OS cell M22_355" "Single U2OS cell M22_356" "Single U2OS cell M22_357" "Single U2OS cell M23_355" "Single U2OS cell M23_356" "Single U2OS cell M23_357" "Single U2OS cell M24_355" "Single U2OS cell M24_356" "Single U2OS cell M24_357" "Single U2OS cell M2_355" "Single U2OS cell M2_356" "Single U2OS cell M2_357" "Single U2OS cell M3_355" "Single U2OS cell M3_356" "Single U2OS cell M3_357" "Single U2OS cell M4_355" "Single U2OS cell M4_356" "Single U2OS cell M4_357" "Single U2OS cell M5_355" "Single U2OS cell M5_356" "Single U2OS cell M5_357" "Single U2OS cell M6_355" "Single U2OS cell M6_356" "Single U2OS cell M6_357" "Single U2OS cell M7_355" "Single U2OS cell M7_356" "Single U2OS cell M7_357" "Single U2OS cell M8_355" "Single U2OS cell M8_356" "Single U2OS cell M8_357" "Single U2OS cell M9_355" "Single U2OS cell M9_356" "Single U2OS cell M9_357" "Single U2OS cell N10_355" "Single U2OS cell N10_356" "Single U2OS cell N10_357" "Single U2OS cell N11_355" "Single U2OS cell N11_356" "Single U2OS cell N11_357" "Single U2OS cell N12_355" "Single U2OS cell N12_356" "Single U2OS cell N12_357" "Single U2OS cell N13_355" "Single U2OS cell N13_356" "Single U2OS cell N13_357" "Single U2OS cell N14_355" "Single U2OS cell N14_356" "Single U2OS cell N14_357" "Single U2OS cell N15_355" "Single U2OS cell N15_356" "Single U2OS cell N15_357" "Single U2OS cell N16_355" "Single U2OS cell N16_356" "Single U2OS cell N16_357" "Single U2OS cell N17_355" "Single U2OS cell N17_356" "Single U2OS cell N17_357" "Single U2OS cell N18_355" "Single U2OS cell N18_356" "Single U2OS cell N18_357" "Single U2OS cell N19_355" "Single U2OS cell N19_356" "Single U2OS cell N19_357" "Single U2OS cell N1_355" "Single U2OS cell N1_356" "Single U2OS cell N1_357" "Single U2OS cell N20_355" "Single U2OS cell N20_356" "Single U2OS cell N20_357" "Single U2OS cell N21_355" "Single U2OS cell N21_356" "Single U2OS cell N21_357" "Single U2OS cell N22_355" "Single U2OS cell N22_356" "Single U2OS cell N22_357" "Single U2OS cell N23_355" "Single U2OS cell N23_356" "Single U2OS cell N23_357" "Single U2OS cell N24_355" "Single U2OS cell N24_356" "Single U2OS cell N24_357" "Single U2OS cell N2_355" "Single U2OS cell N2_356" "Single U2OS cell N2_357" "Single U2OS cell N3_355" "Single U2OS cell N3_356" "Single U2OS cell N3_357" "Single U2OS cell N4_355" "Single U2OS cell N4_356" "Single U2OS cell N4_357" "Single U2OS cell N5_355" "Single U2OS cell N5_356" "Single U2OS cell N5_357" "Single U2OS cell N6_355" "Single U2OS cell N6_356" "Single U2OS cell N6_357" "Single U2OS cell N7_355" "Single U2OS cell N7_356" "Single U2OS cell N7_357" "Single U2OS cell N8_355" "Single U2OS cell N8_356" "Single U2OS cell N8_357" "Single U2OS cell N9_355" "Single U2OS cell N9_356" "Single U2OS cell N9_357" "Single U2OS cell O10_355" "Single U2OS cell O10_356" "Single U2OS cell O10_357" "Single U2OS cell O11_355" "Single U2OS cell O11_356" "Single U2OS cell O11_357" "Single U2OS cell O12_355" "Single U2OS cell O12_356" "Single U2OS cell O12_357" "Single U2OS cell O13_355" "Single U2OS cell O13_356" "Single U2OS cell O13_357" "Single U2OS cell O14_355" "Single U2OS cell O14_356" "Single U2OS cell O14_357" "Single U2OS cell O15_355" "Single U2OS cell O15_356" "Single U2OS cell O15_357" "Single U2OS cell O16_355" "Single U2OS cell O16_356" "Single U2OS cell O16_357" "Single U2OS cell O17_355" "Single U2OS cell O17_356" "Single U2OS cell O17_357" "Single U2OS cell O18_355" "Single U2OS cell O18_356" "Single U2OS cell O18_357" "Single U2OS cell O19_355" "Single U2OS cell O19_356" "Single U2OS cell O19_357" "Single U2OS cell O1_355" "Single U2OS cell O1_356" "Single U2OS cell O1_357" "Single U2OS cell O20_355" "Single U2OS cell O20_356" "Single U2OS cell O20_357" "Single U2OS cell O21_355" "Single U2OS cell O21_356" "Single U2OS cell O21_357" "Single U2OS cell O22_355" "Single U2OS cell O22_356" "Single U2OS cell O22_357" "Single U2OS cell O23_355" "Single U2OS cell O23_356" "Single U2OS cell O23_357" "Single U2OS cell O24_355" "Single U2OS cell O24_356" "Single U2OS cell O24_357" "Single U2OS cell O2_355" "Single U2OS cell O2_356" "Single U2OS cell O2_357" "Single U2OS cell O3_355" "Single U2OS cell O3_356" "Single U2OS cell O3_357" "Single U2OS cell O4_355" "Single U2OS cell O4_356" "Single U2OS cell O4_357" "Single U2OS cell O5_355" "Single U2OS cell O5_356" "Single U2OS cell O5_357" "Single U2OS cell O6_355" "Single U2OS cell O6_356" "Single U2OS cell O6_357" "Single U2OS cell O7_355" "Single U2OS cell O7_356" "Single U2OS cell O7_357" "Single U2OS cell O8_355" "Single U2OS cell O8_356" "Single U2OS cell O8_357" "Single U2OS cell O9_355" "Single U2OS cell O9_356" "Single U2OS cell O9_357" "Single U2OS cell P10_355" "Single U2OS cell P10_356" "Single U2OS cell P10_357" "Single U2OS cell P11_355" "Single U2OS cell P11_356" "Single U2OS cell P11_357" "Single U2OS cell P12_355" "Single U2OS cell P12_356" "Single U2OS cell P12_357" "Single U2OS cell P13_355" "Single U2OS cell P13_356" "Single U2OS cell P13_357" "Single U2OS cell P14_355" "Single U2OS cell P14_356" "Single U2OS cell P14_357" "Single U2OS cell P15_355" "Single U2OS cell P15_356" "Single U2OS cell P15_357" "Single U2OS cell P16_355" "Single U2OS cell P16_356" "Single U2OS cell P16_357" "Single U2OS cell P17_355" "Single U2OS cell P17_356" "Single U2OS cell P17_357" "Single U2OS cell P18_355" "Single U2OS cell P18_356" "Single U2OS cell P18_357" "Single U2OS cell P19_355" "Single U2OS cell P19_356" "Single U2OS cell P19_357" "Single U2OS cell P1_355" "Single U2OS cell P1_356" "Single U2OS cell P1_357" "Single U2OS cell P20_355" "Single U2OS cell P20_356" "Single U2OS cell P20_357" "Single U2OS cell P21_355" "Single U2OS cell P21_356" "Single U2OS cell P21_357" "Single U2OS cell P22_355" "Single U2OS cell P22_356" "Single U2OS cell P22_357" "Single U2OS cell P23_355" "Single U2OS cell P23_356" "Single U2OS cell P23_357" "Single U2OS cell P24_355" "Single U2OS cell P24_356" "Single U2OS cell P24_357" "Single U2OS cell P2_355" "Single U2OS cell P2_356" "Single U2OS cell P2_357" "Single U2OS cell P3_355" "Single U2OS cell P3_356" "Single U2OS cell P3_357" "Single U2OS cell P4_355" "Single U2OS cell P4_356" "Single U2OS cell P4_357" "Single U2OS cell P5_355" "Single U2OS cell P5_356" "Single U2OS cell P5_357" "Single U2OS cell P6_355" "Single U2OS cell P6_356" "Single U2OS cell P6_357" "Single U2OS cell P7_355" "Single U2OS cell P7_356" "Single U2OS cell P7_357" "Single U2OS cell P8_355" "Single U2OS cell P8_356" "Single U2OS cell P8_357" "Single U2OS cell P9_355" "Single U2OS cell P9_356" "Single U2OS cell P9_357" +!Sample_geo_accession "GSM4405639" "GSM4405640" "GSM4405641" "GSM4405642" "GSM4405643" "GSM4405644" "GSM4405645" "GSM4405646" "GSM4405647" "GSM4405648" "GSM4405649" "GSM4405650" "GSM4405651" "GSM4405652" "GSM4405653" "GSM4405654" "GSM4405655" "GSM4405656" "GSM4405657" "GSM4405658" "GSM4405659" "GSM4405660" "GSM4405661" "GSM4405662" "GSM4405663" "GSM4405664" "GSM4405665" "GSM4405666" "GSM4405667" "GSM4405668" "GSM4405669" "GSM4405670" "GSM4405671" "GSM4405672" "GSM4405673" "GSM4405674" "GSM4405675" "GSM4405676" "GSM4405677" "GSM4405678" "GSM4405679" "GSM4405680" "GSM4405681" "GSM4405682" "GSM4405683" "GSM4405684" "GSM4405685" "GSM4405686" "GSM4405687" "GSM4405688" "GSM4405689" "GSM4405690" "GSM4405691" "GSM4405692" "GSM4405693" "GSM4405694" "GSM4405695" "GSM4405696" "GSM4405697" "GSM4405698" "GSM4405699" "GSM4405700" "GSM4405701" "GSM4405702" "GSM4405703" "GSM4405704" "GSM4405705" "GSM4405706" "GSM4405707" "GSM4405708" "GSM4405709" "GSM4405710" "GSM4405711" "GSM4405712" "GSM4405713" "GSM4405714" "GSM4405715" "GSM4405716" "GSM4405717" "GSM4405718" "GSM4405719" "GSM4405720" "GSM4405721" "GSM4405722" "GSM4405723" "GSM4405724" "GSM4405725" "GSM4405726" "GSM4405727" "GSM4405728" "GSM4405729" "GSM4405730" "GSM4405731" "GSM4405732" "GSM4405733" "GSM4405734" "GSM4405735" "GSM4405736" "GSM4405737" "GSM4405738" "GSM4405739" "GSM4405740" "GSM4405741" "GSM4405742" "GSM4405743" "GSM4405744" "GSM4405745" "GSM4405746" "GSM4405747" "GSM4405748" "GSM4405749" "GSM4405750" "GSM4405751" "GSM4405752" "GSM4405753" "GSM4405754" "GSM4405755" "GSM4405756" "GSM4405757" "GSM4405758" "GSM4405759" "GSM4405760" "GSM4405761" "GSM4405762" "GSM4405763" "GSM4405764" "GSM4405765" "GSM4405766" "GSM4405767" "GSM4405768" "GSM4405769" "GSM4405770" "GSM4405771" "GSM4405772" "GSM4405773" "GSM4405774" "GSM4405775" "GSM4405776" "GSM4405777" "GSM4405778" "GSM4405779" "GSM4405780" "GSM4405781" "GSM4405782" "GSM4405783" "GSM4405784" "GSM4405785" "GSM4405786" "GSM4405787" "GSM4405788" "GSM4405789" "GSM4405790" "GSM4405791" "GSM4405792" "GSM4405793" "GSM4405794" "GSM4405795" "GSM4405796" "GSM4405797" "GSM4405798" "GSM4405799" "GSM4405800" "GSM4405801" "GSM4405802" "GSM4405803" "GSM4405804" "GSM4405805" "GSM4405806" "GSM4405807" "GSM4405808" "GSM4405809" "GSM4405810" "GSM4405811" "GSM4405812" "GSM4405813" "GSM4405814" "GSM4405815" "GSM4405816" "GSM4405817" "GSM4405818" "GSM4405819" "GSM4405820" "GSM4405821" "GSM4405822" "GSM4405823" "GSM4405824" "GSM4405825" "GSM4405826" "GSM4405827" "GSM4405828" "GSM4405829" "GSM4405830" "GSM4405831" "GSM4405832" "GSM4405833" "GSM4405834" "GSM4405835" "GSM4405836" "GSM4405837" "GSM4405838" "GSM4405839" "GSM4405840" "GSM4405841" "GSM4405842" "GSM4405843" "GSM4405844" "GSM4405845" "GSM4405846" "GSM4405847" "GSM4405848" "GSM4405849" "GSM4405850" "GSM4405851" "GSM4405852" "GSM4405853" "GSM4405854" "GSM4405855" "GSM4405856" "GSM4405857" "GSM4405858" "GSM4405859" "GSM4405860" "GSM4405861" "GSM4405862" "GSM4405863" "GSM4405864" "GSM4405865" "GSM4405866" "GSM4405867" "GSM4405868" "GSM4405869" "GSM4405870" "GSM4405871" "GSM4405872" "GSM4405873" "GSM4405874" "GSM4405875" "GSM4405876" "GSM4405877" "GSM4405878" "GSM4405879" "GSM4405880" "GSM4405881" "GSM4405882" "GSM4405883" "GSM4405884" "GSM4405885" "GSM4405886" "GSM4405887" "GSM4405888" "GSM4405889" "GSM4405890" "GSM4405891" "GSM4405892" "GSM4405893" "GSM4405894" "GSM4405895" "GSM4405896" "GSM4405897" "GSM4405898" "GSM4405899" "GSM4405900" "GSM4405901" "GSM4405902" "GSM4405903" "GSM4405904" "GSM4405905" "GSM4405906" "GSM4405907" "GSM4405908" "GSM4405909" "GSM4405910" "GSM4405911" "GSM4405912" "GSM4405913" "GSM4405914" "GSM4405915" "GSM4405916" "GSM4405917" "GSM4405918" "GSM4405919" "GSM4405920" "GSM4405921" "GSM4405922" "GSM4405923" "GSM4405924" "GSM4405925" "GSM4405926" "GSM4405927" "GSM4405928" "GSM4405929" "GSM4405930" "GSM4405931" "GSM4405932" "GSM4405933" "GSM4405934" "GSM4405935" "GSM4405936" "GSM4405937" "GSM4405938" "GSM4405939" "GSM4405940" "GSM4405941" "GSM4405942" "GSM4405943" "GSM4405944" "GSM4405945" "GSM4405946" "GSM4405947" "GSM4405948" "GSM4405949" "GSM4405950" "GSM4405951" "GSM4405952" "GSM4405953" "GSM4405954" "GSM4405955" "GSM4405956" "GSM4405957" "GSM4405958" "GSM4405959" "GSM4405960" "GSM4405961" "GSM4405962" "GSM4405963" "GSM4405964" "GSM4405965" "GSM4405966" "GSM4405967" "GSM4405968" "GSM4405969" "GSM4405970" "GSM4405971" "GSM4405972" "GSM4405973" "GSM4405974" "GSM4405975" "GSM4405976" "GSM4405977" "GSM4405978" "GSM4405979" "GSM4405980" "GSM4405981" "GSM4405982" "GSM4405983" "GSM4405984" "GSM4405985" "GSM4405986" "GSM4405987" "GSM4405988" "GSM4405989" "GSM4405990" "GSM4405991" "GSM4405992" "GSM4405993" "GSM4405994" "GSM4405995" "GSM4405996" "GSM4405997" "GSM4405998" "GSM4405999" "GSM4406000" "GSM4406001" "GSM4406002" "GSM4406003" "GSM4406004" "GSM4406005" "GSM4406006" "GSM4406007" "GSM4406008" "GSM4406009" "GSM4406010" "GSM4406011" "GSM4406012" "GSM4406013" "GSM4406014" "GSM4406015" "GSM4406016" "GSM4406017" "GSM4406018" "GSM4406019" "GSM4406020" "GSM4406021" "GSM4406022" "GSM4406023" "GSM4406024" "GSM4406025" "GSM4406026" "GSM4406027" "GSM4406028" "GSM4406029" "GSM4406030" "GSM4406031" "GSM4406032" "GSM4406033" "GSM4406034" "GSM4406035" "GSM4406036" "GSM4406037" "GSM4406038" "GSM4406039" "GSM4406040" "GSM4406041" "GSM4406042" "GSM4406043" "GSM4406044" "GSM4406045" "GSM4406046" "GSM4406047" "GSM4406048" "GSM4406049" "GSM4406050" "GSM4406051" "GSM4406052" "GSM4406053" "GSM4406054" "GSM4406055" "GSM4406056" "GSM4406057" "GSM4406058" "GSM4406059" "GSM4406060" "GSM4406061" "GSM4406062" "GSM4406063" "GSM4406064" "GSM4406065" "GSM4406066" "GSM4406067" "GSM4406068" "GSM4406069" "GSM4406070" "GSM4406071" "GSM4406072" "GSM4406073" "GSM4406074" "GSM4406075" "GSM4406076" "GSM4406077" "GSM4406078" "GSM4406079" "GSM4406080" "GSM4406081" "GSM4406082" "GSM4406083" "GSM4406084" "GSM4406085" "GSM4406086" "GSM4406087" "GSM4406088" "GSM4406089" "GSM4406090" "GSM4406091" "GSM4406092" "GSM4406093" "GSM4406094" "GSM4406095" "GSM4406096" "GSM4406097" "GSM4406098" "GSM4406099" "GSM4406100" "GSM4406101" "GSM4406102" "GSM4406103" "GSM4406104" "GSM4406105" "GSM4406106" "GSM4406107" "GSM4406108" "GSM4406109" "GSM4406110" "GSM4406111" "GSM4406112" "GSM4406113" "GSM4406114" "GSM4406115" "GSM4406116" "GSM4406117" "GSM4406118" "GSM4406119" "GSM4406120" "GSM4406121" "GSM4406122" "GSM4406123" "GSM4406124" "GSM4406125" "GSM4406126" "GSM4406127" "GSM4406128" "GSM4406129" "GSM4406130" "GSM4406131" "GSM4406132" "GSM4406133" "GSM4406134" "GSM4406135" "GSM4406136" "GSM4406137" "GSM4406138" "GSM4406139" "GSM4406140" "GSM4406141" "GSM4406142" "GSM4406143" "GSM4406144" "GSM4406145" "GSM4406146" "GSM4406147" "GSM4406148" "GSM4406149" "GSM4406150" "GSM4406151" "GSM4406152" "GSM4406153" "GSM4406154" "GSM4406155" "GSM4406156" "GSM4406157" "GSM4406158" "GSM4406159" "GSM4406160" "GSM4406161" "GSM4406162" "GSM4406163" "GSM4406164" "GSM4406165" "GSM4406166" "GSM4406167" "GSM4406168" "GSM4406169" "GSM4406170" "GSM4406171" "GSM4406172" "GSM4406173" "GSM4406174" "GSM4406175" "GSM4406176" "GSM4406177" "GSM4406178" "GSM4406179" "GSM4406180" "GSM4406181" "GSM4406182" "GSM4406183" "GSM4406184" "GSM4406185" "GSM4406186" "GSM4406187" "GSM4406188" "GSM4406189" "GSM4406190" "GSM4406191" "GSM4406192" "GSM4406193" "GSM4406194" "GSM4406195" "GSM4406196" "GSM4406197" "GSM4406198" "GSM4406199" "GSM4406200" "GSM4406201" "GSM4406202" "GSM4406203" "GSM4406204" "GSM4406205" "GSM4406206" "GSM4406207" "GSM4406208" "GSM4406209" "GSM4406210" "GSM4406211" "GSM4406212" "GSM4406213" "GSM4406214" "GSM4406215" "GSM4406216" "GSM4406217" "GSM4406218" "GSM4406219" "GSM4406220" "GSM4406221" "GSM4406222" "GSM4406223" "GSM4406224" "GSM4406225" "GSM4406226" "GSM4406227" "GSM4406228" "GSM4406229" "GSM4406230" "GSM4406231" "GSM4406232" "GSM4406233" "GSM4406234" "GSM4406235" "GSM4406236" "GSM4406237" "GSM4406238" "GSM4406239" "GSM4406240" "GSM4406241" "GSM4406242" "GSM4406243" "GSM4406244" "GSM4406245" "GSM4406246" "GSM4406247" "GSM4406248" "GSM4406249" "GSM4406250" "GSM4406251" "GSM4406252" "GSM4406253" "GSM4406254" "GSM4406255" "GSM4406256" "GSM4406257" "GSM4406258" "GSM4406259" "GSM4406260" "GSM4406261" "GSM4406262" "GSM4406263" "GSM4406264" "GSM4406265" "GSM4406266" "GSM4406267" "GSM4406268" "GSM4406269" "GSM4406270" "GSM4406271" "GSM4406272" "GSM4406273" "GSM4406274" "GSM4406275" "GSM4406276" "GSM4406277" "GSM4406278" "GSM4406279" "GSM4406280" "GSM4406281" "GSM4406282" "GSM4406283" "GSM4406284" "GSM4406285" "GSM4406286" "GSM4406287" "GSM4406288" "GSM4406289" "GSM4406290" "GSM4406291" "GSM4406292" "GSM4406293" "GSM4406294" "GSM4406295" "GSM4406296" "GSM4406297" "GSM4406298" "GSM4406299" "GSM4406300" "GSM4406301" "GSM4406302" "GSM4406303" "GSM4406304" "GSM4406305" "GSM4406306" "GSM4406307" "GSM4406308" "GSM4406309" "GSM4406310" "GSM4406311" "GSM4406312" "GSM4406313" "GSM4406314" "GSM4406315" "GSM4406316" "GSM4406317" "GSM4406318" "GSM4406319" "GSM4406320" "GSM4406321" "GSM4406322" "GSM4406323" "GSM4406324" "GSM4406325" "GSM4406326" "GSM4406327" "GSM4406328" "GSM4406329" "GSM4406330" "GSM4406331" "GSM4406332" "GSM4406333" "GSM4406334" "GSM4406335" "GSM4406336" "GSM4406337" "GSM4406338" "GSM4406339" "GSM4406340" "GSM4406341" "GSM4406342" "GSM4406343" "GSM4406344" "GSM4406345" "GSM4406346" "GSM4406347" "GSM4406348" "GSM4406349" "GSM4406350" "GSM4406351" "GSM4406352" "GSM4406353" "GSM4406354" "GSM4406355" "GSM4406356" "GSM4406357" "GSM4406358" "GSM4406359" "GSM4406360" "GSM4406361" "GSM4406362" "GSM4406363" "GSM4406364" "GSM4406365" "GSM4406366" "GSM4406367" "GSM4406368" "GSM4406369" "GSM4406370" "GSM4406371" "GSM4406372" "GSM4406373" "GSM4406374" "GSM4406375" "GSM4406376" "GSM4406377" "GSM4406378" "GSM4406379" "GSM4406380" "GSM4406381" "GSM4406382" "GSM4406383" "GSM4406384" "GSM4406385" "GSM4406386" "GSM4406387" "GSM4406388" "GSM4406389" "GSM4406390" "GSM4406391" "GSM4406392" "GSM4406393" "GSM4406394" "GSM4406395" "GSM4406396" "GSM4406397" "GSM4406398" "GSM4406399" "GSM4406400" "GSM4406401" "GSM4406402" "GSM4406403" "GSM4406404" "GSM4406405" "GSM4406406" "GSM4406407" "GSM4406408" "GSM4406409" "GSM4406410" "GSM4406411" "GSM4406412" "GSM4406413" "GSM4406414" "GSM4406415" "GSM4406416" "GSM4406417" "GSM4406418" "GSM4406419" "GSM4406420" "GSM4406421" "GSM4406422" "GSM4406423" "GSM4406424" "GSM4406425" "GSM4406426" "GSM4406427" "GSM4406428" "GSM4406429" "GSM4406430" "GSM4406431" "GSM4406432" "GSM4406433" "GSM4406434" "GSM4406435" "GSM4406436" "GSM4406437" "GSM4406438" "GSM4406439" "GSM4406440" "GSM4406441" "GSM4406442" "GSM4406443" "GSM4406444" "GSM4406445" "GSM4406446" "GSM4406447" "GSM4406448" "GSM4406449" "GSM4406450" "GSM4406451" "GSM4406452" "GSM4406453" "GSM4406454" "GSM4406455" "GSM4406456" "GSM4406457" "GSM4406458" "GSM4406459" "GSM4406460" "GSM4406461" "GSM4406462" "GSM4406463" "GSM4406464" "GSM4406465" "GSM4406466" "GSM4406467" "GSM4406468" "GSM4406469" "GSM4406470" "GSM4406471" "GSM4406472" "GSM4406473" "GSM4406474" "GSM4406475" "GSM4406476" "GSM4406477" "GSM4406478" "GSM4406479" "GSM4406480" "GSM4406481" "GSM4406482" "GSM4406483" "GSM4406484" "GSM4406485" "GSM4406486" "GSM4406487" "GSM4406488" "GSM4406489" "GSM4406490" "GSM4406491" "GSM4406492" "GSM4406493" "GSM4406494" "GSM4406495" "GSM4406496" "GSM4406497" "GSM4406498" "GSM4406499" "GSM4406500" "GSM4406501" "GSM4406502" "GSM4406503" "GSM4406504" "GSM4406505" "GSM4406506" "GSM4406507" "GSM4406508" "GSM4406509" "GSM4406510" "GSM4406511" "GSM4406512" "GSM4406513" "GSM4406514" "GSM4406515" "GSM4406516" "GSM4406517" "GSM4406518" "GSM4406519" "GSM4406520" "GSM4406521" "GSM4406522" "GSM4406523" "GSM4406524" "GSM4406525" "GSM4406526" "GSM4406527" "GSM4406528" "GSM4406529" "GSM4406530" "GSM4406531" "GSM4406532" "GSM4406533" "GSM4406534" "GSM4406535" "GSM4406536" "GSM4406537" "GSM4406538" "GSM4406539" 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line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" "cell line: U2OS" +!Sample_growth_protocol_ch1 "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." "U-2 OS FUCCI cells were kindly provided by Dr. Miyawaki. These cells are endogenously tagged with two fluorescent proteins fused to cell cycle regulators to allow cell cycle monitoring; CDT1 (mKO2-hCdt1+) accumulates in G1 phase, while Geminin (mAG-hGem+) accumulates in S and G2 phases. Cells expressing FUCCI probes are divided into red mKO2(+)mAG(-), yellow mKO2(+)mAG(+), and green mKO2(-)mAG(+) emitting populations. The cells were cultivated in Petri dishes at 37 °C in a 5.0 % CO2 humidified environment in McCoy’s 5A (modified) medium GlutaMAX supplement, (ThermoFisher, 36600021, MA, USA) supplemented with 10% fetal bovine serum (FBS, VWR, Radnor, PA, USA) without antibiotics. The cells were maintained sub-confluent and harvested by trypsinization at log-phase growth (60% confluency) for subsequent analysis." +!Sample_molecule_ch1 "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" "polyA RNA" 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"polyA RNA" "polyA RNA" "polyA RNA" +!Sample_extract_protocol_ch1 "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." "We used the polyA-RNA extraction method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013." +!Sample_extract_protocol_ch1 "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." "We used the cDNA library preparation method from the SMART-Seq2 protocol by Picelli, et al. Nature Methods 2013, along with ERCC spike-in sequences." +!Sample_taxid_ch1 "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" "9606" 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"Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." "Alignment and quantification were performed with RSEM (version 1.3.2-0) using STAR (version 2.7.0f-0." +!Sample_data_processing "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" "Genome_build: GRCh38.98" 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"Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." "Supplementary_files_format_and_content: Comma-delimited files with TPM or counts for all samples or for all ERCC spike-in sequences." 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"GSM4406217" "GSM4406218" "GSM4406219" "GSM4406220" "GSM4406221" "GSM4406222" "GSM4406223" "GSM4406224" "GSM4406225" "GSM4406226" "GSM4406227" "GSM4406228" "GSM4406229" "GSM4406230" "GSM4406231" "GSM4406232" "GSM4406233" "GSM4406234" "GSM4406235" "GSM4406236" "GSM4406237" "GSM4406238" "GSM4406239" "GSM4406240" "GSM4406241" "GSM4406242" "GSM4406243" "GSM4406244" "GSM4406245" "GSM4406246" "GSM4406247" "GSM4406248" "GSM4406249" "GSM4406250" "GSM4406251" "GSM4406252" "GSM4406253" "GSM4406254" "GSM4406255" "GSM4406256" "GSM4406257" "GSM4406258" "GSM4406259" "GSM4406260" "GSM4406261" "GSM4406262" "GSM4406263" "GSM4406264" "GSM4406265" "GSM4406266" "GSM4406267" "GSM4406268" "GSM4406269" "GSM4406270" "GSM4406271" "GSM4406272" "GSM4406273" "GSM4406274" "GSM4406275" "GSM4406276" "GSM4406277" "GSM4406278" "GSM4406279" "GSM4406280" "GSM4406281" "GSM4406282" "GSM4406283" "GSM4406284" "GSM4406285" "GSM4406286" "GSM4406287" "GSM4406288" "GSM4406289" "GSM4406290" "GSM4406291" "GSM4406292" "GSM4406293" "GSM4406294" "GSM4406295" "GSM4406296" "GSM4406297" "GSM4406298" "GSM4406299" "GSM4406300" "GSM4406301" "GSM4406302" "GSM4406303" "GSM4406304" "GSM4406305" "GSM4406306" "GSM4406307" "GSM4406308" "GSM4406309" "GSM4406310" "GSM4406311" "GSM4406312" "GSM4406313" "GSM4406314" "GSM4406315" "GSM4406316" "GSM4406317" "GSM4406318" "GSM4406319" "GSM4406320" "GSM4406321" "GSM4406322" "GSM4406323" "GSM4406324" "GSM4406325" "GSM4406326" "GSM4406327" "GSM4406328" "GSM4406329" "GSM4406330" "GSM4406331" "GSM4406332" "GSM4406333" "GSM4406334" "GSM4406335" "GSM4406336" "GSM4406337" "GSM4406338" "GSM4406339" "GSM4406340" "GSM4406341" "GSM4406342" "GSM4406343" "GSM4406344" "GSM4406345" "GSM4406346" "GSM4406347" "GSM4406348" "GSM4406349" "GSM4406350" "GSM4406351" "GSM4406352" "GSM4406353" "GSM4406354" "GSM4406355" "GSM4406356" "GSM4406357" "GSM4406358" "GSM4406359" "GSM4406360" "GSM4406361" "GSM4406362" "GSM4406363" "GSM4406364" "GSM4406365" "GSM4406366" "GSM4406367" "GSM4406368" "GSM4406369" "GSM4406370" "GSM4406371" "GSM4406372" "GSM4406373" "GSM4406374" "GSM4406375" "GSM4406376" "GSM4406377" "GSM4406378" "GSM4406379" "GSM4406380" "GSM4406381" "GSM4406382" "GSM4406383" "GSM4406384" "GSM4406385" "GSM4406386" "GSM4406387" "GSM4406388" "GSM4406389" "GSM4406390" "GSM4406391" "GSM4406392" "GSM4406393" "GSM4406394" "GSM4406395" "GSM4406396" "GSM4406397" "GSM4406398" "GSM4406399" "GSM4406400" "GSM4406401" "GSM4406402" "GSM4406403" "GSM4406404" "GSM4406405" "GSM4406406" "GSM4406407" "GSM4406408" "GSM4406409" "GSM4406410" "GSM4406411" "GSM4406412" "GSM4406413" "GSM4406414" "GSM4406415" "GSM4406416" "GSM4406417" "GSM4406418" "GSM4406419" "GSM4406420" "GSM4406421" "GSM4406422" "GSM4406423" "GSM4406424" "GSM4406425" "GSM4406426" "GSM4406427" "GSM4406428" "GSM4406429" "GSM4406430" "GSM4406431" "GSM4406432" "GSM4406433" "GSM4406434" "GSM4406435" "GSM4406436" "GSM4406437" "GSM4406438" "GSM4406439" "GSM4406440" "GSM4406441" "GSM4406442" "GSM4406443" "GSM4406444" "GSM4406445" "GSM4406446" "GSM4406447" "GSM4406448" "GSM4406449" "GSM4406450" "GSM4406451" "GSM4406452" "GSM4406453" "GSM4406454" "GSM4406455" "GSM4406456" "GSM4406457" "GSM4406458" "GSM4406459" "GSM4406460" "GSM4406461" "GSM4406462" "GSM4406463" "GSM4406464" "GSM4406465" "GSM4406466" "GSM4406467" "GSM4406468" "GSM4406469" "GSM4406470" "GSM4406471" "GSM4406472" "GSM4406473" "GSM4406474" "GSM4406475" "GSM4406476" "GSM4406477" "GSM4406478" "GSM4406479" "GSM4406480" "GSM4406481" "GSM4406482" "GSM4406483" "GSM4406484" "GSM4406485" "GSM4406486" "GSM4406487" "GSM4406488" "GSM4406489" "GSM4406490" "GSM4406491" "GSM4406492" "GSM4406493" "GSM4406494" "GSM4406495" "GSM4406496" "GSM4406497" "GSM4406498" "GSM4406499" "GSM4406500" "GSM4406501" "GSM4406502" "GSM4406503" "GSM4406504" "GSM4406505" "GSM4406506" "GSM4406507" "GSM4406508" "GSM4406509" "GSM4406510" "GSM4406511" "GSM4406512" "GSM4406513" "GSM4406514" "GSM4406515" "GSM4406516" "GSM4406517" "GSM4406518" "GSM4406519" "GSM4406520" "GSM4406521" "GSM4406522" "GSM4406523" "GSM4406524" "GSM4406525" "GSM4406526" "GSM4406527" "GSM4406528" "GSM4406529" "GSM4406530" "GSM4406531" "GSM4406532" "GSM4406533" "GSM4406534" "GSM4406535" "GSM4406536" "GSM4406537" "GSM4406538" "GSM4406539" "GSM4406540" "GSM4406541" "GSM4406542" "GSM4406543" "GSM4406544" "GSM4406545" "GSM4406546" "GSM4406547" "GSM4406548" "GSM4406549" "GSM4406550" "GSM4406551" "GSM4406552" "GSM4406553" "GSM4406554" "GSM4406555" "GSM4406556" "GSM4406557" "GSM4406558" "GSM4406559" "GSM4406560" "GSM4406561" "GSM4406562" "GSM4406563" "GSM4406564" "GSM4406565" "GSM4406566" "GSM4406567" "GSM4406568" "GSM4406569" "GSM4406570" "GSM4406571" "GSM4406572" "GSM4406573" "GSM4406574" "GSM4406575" "GSM4406576" "GSM4406577" "GSM4406578" "GSM4406579" "GSM4406580" "GSM4406581" "GSM4406582" "GSM4406583" "GSM4406584" "GSM4406585" "GSM4406586" "GSM4406587" "GSM4406588" "GSM4406589" "GSM4406590" "GSM4406591" "GSM4406592" "GSM4406593" "GSM4406594" "GSM4406595" "GSM4406596" "GSM4406597" "GSM4406598" "GSM4406599" "GSM4406600" "GSM4406601" "GSM4406602" "GSM4406603" "GSM4406604" "GSM4406605" "GSM4406606" "GSM4406607" "GSM4406608" "GSM4406609" "GSM4406610" "GSM4406611" "GSM4406612" "GSM4406613" "GSM4406614" "GSM4406615" "GSM4406616" "GSM4406617" "GSM4406618" "GSM4406619" "GSM4406620" "GSM4406621" "GSM4406622" "GSM4406623" "GSM4406624" "GSM4406625" "GSM4406626" "GSM4406627" "GSM4406628" "GSM4406629" "GSM4406630" "GSM4406631" "GSM4406632" "GSM4406633" "GSM4406634" "GSM4406635" "GSM4406636" "GSM4406637" "GSM4406638" "GSM4406639" "GSM4406640" "GSM4406641" "GSM4406642" "GSM4406643" "GSM4406644" "GSM4406645" "GSM4406646" "GSM4406647" "GSM4406648" "GSM4406649" "GSM4406650" "GSM4406651" "GSM4406652" "GSM4406653" "GSM4406654" "GSM4406655" "GSM4406656" "GSM4406657" "GSM4406658" "GSM4406659" "GSM4406660" "GSM4406661" "GSM4406662" "GSM4406663" "GSM4406664" "GSM4406665" "GSM4406666" "GSM4406667" "GSM4406668" "GSM4406669" "GSM4406670" "GSM4406671" "GSM4406672" "GSM4406673" "GSM4406674" "GSM4406675" "GSM4406676" "GSM4406677" "GSM4406678" "GSM4406679" "GSM4406680" "GSM4406681" "GSM4406682" "GSM4406683" "GSM4406684" "GSM4406685" "GSM4406686" "GSM4406687" "GSM4406688" "GSM4406689" "GSM4406690" "GSM4406691" "GSM4406692" "GSM4406693" "GSM4406694" "GSM4406695" "GSM4406696" "GSM4406697" "GSM4406698" "GSM4406699" "GSM4406700" "GSM4406701" "GSM4406702" "GSM4406703" "GSM4406704" "GSM4406705" "GSM4406706" "GSM4406707" "GSM4406708" "GSM4406709" "GSM4406710" "GSM4406711" "GSM4406712" "GSM4406713" "GSM4406714" "GSM4406715" "GSM4406716" "GSM4406717" "GSM4406718" "GSM4406719" "GSM4406720" "GSM4406721" "GSM4406722" "GSM4406723" "GSM4406724" "GSM4406725" "GSM4406726" "GSM4406727" "GSM4406728" "GSM4406729" "GSM4406730" "GSM4406731" "GSM4406732" "GSM4406733" "GSM4406734" "GSM4406735" "GSM4406736" "GSM4406737" "GSM4406738" "GSM4406739" "GSM4406740" "GSM4406741" "GSM4406742" "GSM4406743" "GSM4406744" "GSM4406745" "GSM4406746" "GSM4406747" "GSM4406748" "GSM4406749" "GSM4406750" "GSM4406751" "GSM4406752" "GSM4406753" "GSM4406754" "GSM4406755" "GSM4406756" "GSM4406757" "GSM4406758" "GSM4406759" "GSM4406760" "GSM4406761" "GSM4406762" "GSM4406763" "GSM4406764" "GSM4406765" "GSM4406766" "GSM4406767" "GSM4406768" "GSM4406769" "GSM4406770" "GSM4406771" "GSM4406772" "GSM4406773" "GSM4406774" "GSM4406775" "GSM4406776" "GSM4406777" "GSM4406778" "GSM4406779" "GSM4406780" "GSM4406781" "GSM4406782" "GSM4406783" "GSM4406784" "GSM4406785" "GSM4406786" "GSM4406787" "GSM4406788" "GSM4406789" "GSM4406790" +!series_matrix_table_end diff --git a/rules/quant.smk b/rules/quant.smk deleted file mode 100644 index 1d37e9b..0000000 --- a/rules/quant.smk +++ /dev/null @@ -1,102 +0,0 @@ -rule rsem_star_genome: - '''Create an RSEM reference with STAR indices''' - input: - efa="data/ERCC.fa", - gfa=FA, - gff=GFF3 + ".fix.gff3" - output: - REFSTAR_PREFIX + ".gtf", - suffix = REFSTAR_FOLDER + "SA" - threads: 99 - resources: mem_mb=60000 - log: "ensembl/prepare-reference.log" - shell: - "(rsem-prepare-reference --num-threads {threads} --star --gff3 {input.gff}" - " \"{input.efa}\",\"{input.gfa}\" " + REFSTAR_PREFIX + - ") 2> {log}" - -rule rsem_star_align: - '''Align to transcripts with STAR and quantify with RSEM''' - input: - suffix=REFSTAR_FOLDER + "SA", - gtf=REFSTAR_PREFIX + ".gtf", - fastq=expand(FQ_FOLDER + "{fq}.fastq.gz", fq=FQ_PREFIXES) #single end only - output: - "output/{fq}.isoforms.results", - "output/{fq}.genes.results", - "output/{fq}.time", - directory("output/{fq}.stat"), - resources: mem_mb=50000 - threads: 12 - log: "output/{fq}calculate-expression.log" - shell: - "(rsem-calculate-expression --no-bam-output --time --star" # --calc-ci" probably not using confidence intervals here - " --num-threads {threads} <(zcat " + FQ_FOLDER + "{wildcards.fq}.fastq.gz) " + REFSTAR_PREFIX + " output/{wildcards.fq}) &> {log}" - -rule make_gene_rsem_dataframe: - '''Take the results from RSEM and put them in a usable dataframe''' - input: - expand("output/{fq}.genes.results", fq=FQ_PREFIXES), - gff=GFF3 + ".fix.gff3" - output: - counts="output/Counts.csv", - names="output/IdsToNames.csv", - tpms="output/Tpms.csv" - shell: - "python scripts/make_rsem_dataframe.py genes {input.gff} {output.counts} {output.tpms} {output.names}" - -rule make_isoform_rsem_dataframe: - '''Take the results from RSEM and put them in a usable dataframe''' - input: - expand("output/{fq}.isoforms.results", fq=FQ_PREFIXES), - gff=GFF3 + ".fix.gff3" - output: - counts="output/Counts_Isoforms.csv", - names="output/IdsToNames_Isoforms.csv", - tpms="output/Tpms_Isoforms.csv" - shell: - "python scripts/make_rsem_dataframe.py isoforms {input.gff} {output.counts} {output.tpms} {output.names}" - -# rule rsem_genome: -# '''Create an RSEM reference. For use with STAR aligned BAMs.''' -# input: -# efa="data/ERCC.fa", -# gfa=FA, -# gff=GFF3 + ".fix.gff3" -# output: -# REF_PREFIX + ".gtf", -# threads: 12 -# resources: mem_mb=60000 -# log: "ensembl/prepare-reference.log" -# shell: -# "(rsem-prepare-reference --num-threads {threads} --gff3 {input.gff}" -# " \"{input.efa}\",\"{input.gfa}\" " + REF_PREFIX + -# ") 2> {log}" -# -# rule rsem_bam: -# input: -# REF_PREFIX + ".gtf", -# temp("temp/unloaded_2pass"), # trigger unload star reference -# "output/{fq}" + unique_tag() + "Aligned.sortedByCoord.out.bam" -# output: -# "output/{fq}" + unique_tag() + ".isoforms.results", -# "output/{fq}" + unique_tag() + ".genes.results", -# "output/{fq}" + unique_tag() + ".time", -# directory("output/{fq}.stat"), -# resources: mem_mb=50000 -# threads: 12 -# log: "output/{fq}calculate-expression.log" -# shell: -# "(rsem-calculate-expression --no-bam-output --time" # --calc-ci" probably not using confidence intervals here -# " --num-threads {threads} --alignments output/{wildcards.fq}" + unique_tag() + "Aligned.sortedByCoord.out.bam " + -# REF_PREFIX + " output/{wildcards.fq}) &> {log}" - -# rule make_rsem_dataframe: -# input: expand("output/{fq}" + unique_tag() + ".genes.results", fq=FQ_PREFIXES) -# output: -# counts="output/counts" + unique_tag() + ".tsv", -# tpm="output/tpm" + unique_tag() + ".tsv" -# shell: -# "echo \"python scripts/make_rsem_dataframe.py 4 {output.counts} {input}\" && " -# "python scripts/make_rsem_dataframe.py 4 {output.counts} {input} && " -# "python scripts/make_rsem_dataframe.py 5 {output.tpm} {input}" diff --git a/workflow/Snakefile b/workflow/Snakefile new file mode 100644 index 0000000..441b21c --- /dev/null +++ b/workflow/Snakefile @@ -0,0 +1,33 @@ +configfile: "config/SraAccList.yaml" + +GENOME_VERSION = "GRCh38" +ENSEMBL_VERSION = "103" +SPECIES = config["species"] +SPECIES_LOWER = SPECIES.lower() +REF = f"{SPECIES}.{GENOME_VERSION}" +GENEMODEL_VERSION = f"{GENOME_VERSION}.{ENSEMBL_VERSION}" +GENOME_FA = f"../resources/ensembl/Homo_sapiens.{GENOME_VERSION}.dna.primary_assembly.fa" +ENSEMBL_GFF = f"../resources/ensembl/Homo_sapiens.{GENEMODEL_VERSION}.gff3" +ENSEMBL_GTF = f"../resources/ensembl/Homo_sapiens.{GENEMODEL_VERSION}.gtf" +RSEM_REF_FOLDER = f"../resources/ensembl/Homo_sapiens.{GENEMODEL_VERSION}Rsem/" +STAR_REF_FOLDER = f"../resources/ensembl/Homo_sapiens.{GENEMODEL_VERSION}Star" # no slash +FA,GFF,GTF=GENOME_FA,ENSEMBL_GFF,ENSEMBL_GTF + +# Test files +TEST_GENOME_FA = f"../resources/ensembl/202122.fa" +TEST_ENSEMBL_GFF = f"../resources/ensembl/202122.gff3" +TEST_ENSEMBL_GTF = f"../resources/ensembl/202122.gtf" + +# Uncomment to trigger testing +#FA,GFF,GTF=TEST_GENOME_FA,TEST_ENSEMBL_GFF,TEST_ENSEMBL_GTF + +rule all: + input: + "../results/final/ProteinPseudotimePlotting.csv.gz", + "../results/final/RNAPseudotimePlotting.csv.gz", + +include: "rules/downloads.smk" +include: "rules/align.smk" +include: "rules/quant.smk" +include: "rules/velo.smk" +include: "rules/singlecellproteogenomics.smk" diff --git a/workflow/config/SraAccList.yaml b/workflow/config/SraAccList.yaml new file mode 100644 index 0000000..4321d20 --- /dev/null +++ b/workflow/config/SraAccList.yaml @@ -0,0 +1,1154 @@ +species: "Homo_sapiens" # ensembl species name +sra: + - SRR11286626 + - SRR11286627 + - SRR11286628 + - SRR11286629 + - SRR11286630 + - SRR11286631 + - SRR11286632 + - SRR11286633 + - SRR11286634 + - SRR11286635 + - SRR11286636 + - SRR11286637 + - SRR11286638 + - SRR11286639 + - SRR11286640 + - SRR11286641 + - SRR11286642 + - SRR11286643 + - SRR11286644 + - SRR11286645 + - SRR11286646 + - SRR11286647 + - SRR11286648 + - SRR11286649 + - SRR11286650 + - SRR11286651 + - SRR11286652 + - SRR11286653 + - SRR11286654 + - SRR11286655 + - SRR11286656 + - SRR11286657 + - SRR11286658 + - SRR11286659 + - SRR11286660 + - SRR11286661 + - SRR11286662 + - SRR11286663 + - SRR11286664 + - SRR11286665 + - SRR11286666 + - SRR11286667 + - SRR11286668 + - SRR11286669 + - SRR11286670 + - SRR11286671 + - SRR11286672 + - SRR11286673 + - SRR11286674 + - SRR11286675 + - SRR11286676 + - SRR11286677 + - SRR11286678 + - SRR11286679 + - SRR11286680 + - SRR11286681 + - SRR11286682 + - SRR11286683 + - SRR11286684 + - SRR11286685 + - SRR11286686 + - SRR11286687 + - SRR11286688 + - SRR11286689 + - SRR11286690 + - SRR11286691 + - SRR11286692 + - SRR11286693 + - SRR11286694 + - SRR11286695 + - SRR11286696 + - SRR11286697 + - SRR11286698 + - SRR11286699 + - SRR11286700 + - SRR11286701 + - SRR11286702 + - SRR11286703 + - SRR11286704 + - SRR11286705 + - SRR11286706 + - SRR11286707 + - SRR11286708 + - SRR11286709 + - SRR11286710 + - SRR11286711 + - SRR11286712 + - SRR11286713 + - SRR11286714 + - SRR11286715 + - SRR11286716 + - SRR11286717 + - SRR11286718 + - SRR11286719 + - SRR11286720 + - SRR11286721 + - SRR11287102 + - SRR11287103 + - SRR11287104 + - SRR11287105 + - SRR11286786 + - SRR11286787 + - SRR11286788 + - SRR11286789 + - SRR11286790 + - SRR11286791 + - 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b/workflow/envs/downloads.yaml @@ -0,0 +1,12 @@ +channels: + - bioconda + - conda-forge +dependencies: + - python + - fastp + - sra-tools =2.10 + - biopython + - pandas + - gzip + - wget + - gdown diff --git a/workflow/envs/environment.yaml b/workflow/envs/environment.yaml new file mode 100644 index 0000000..7af0285 --- /dev/null +++ b/workflow/envs/environment.yaml @@ -0,0 +1,21 @@ +channels: + - bioconda + - conda-forge +dependencies: + - python + - numpy + - pandas + - rsem =1.3.3 + - star =2.7.9a + - git + - fastp + - sra-tools =2.10 + - biopython + - gzip + - wget + - gdown + - scvelo==0.2.3 + - velocyto.py==0.17.17 + - pytables # scvelo + - louvain # sccvelo + - entrez-direct # for srr_lookup diff --git a/workflow/envs/quant.yaml b/workflow/envs/quant.yaml new file mode 100644 index 0000000..77b005f --- /dev/null +++ b/workflow/envs/quant.yaml @@ -0,0 +1,9 @@ +channels: + - bioconda + - conda-forge +dependencies: + - python + - numpy + - pandas + - rsem =1.3.3 + - star =2.7.9a diff --git a/workflow/envs/setup.yaml b/workflow/envs/setup.yaml new file mode 100644 index 0000000..2d214fb --- /dev/null +++ b/workflow/envs/setup.yaml @@ -0,0 +1,4 @@ +channels: + - conda-forge +dependencies: + - snakemake-minimal diff --git a/workflow/envs/velo.yaml b/workflow/envs/velo.yaml new file mode 100644 index 0000000..63d48aa --- /dev/null +++ b/workflow/envs/velo.yaml @@ -0,0 +1,5 @@ +channels: + - bioconda +dependencies: + - velocyto.py==0.17.17 + - entrez-direct # for srr_lookup diff --git a/workflow/rules/align.smk b/workflow/rules/align.smk new file mode 100644 index 0000000..83ee3ea --- /dev/null +++ b/workflow/rules/align.smk @@ -0,0 +1,137 @@ +RAM_MB_REQ = 50000 #mb +RAM_B_REQ = RAM_MB_REQ * 1000 # BYTES + +rule star_genome_generate: + input: + efa="../resources/ERCC.filtered.fa", + gfa=FA, + gtf=f"{GTF}.fix.gtf" + output: f"{STAR_REF_FOLDER}/SA" + params: + genomedir=lambda w, output: os.path.dirname(output[0]), + size="--genomeSAindexNbases 12" if FA == TEST_GENOME_FA else "", + sjoptions="--sjdbOverhang 42" # 43bp reads for this experiment + threads: 99 + log: f"{STAR_REF_FOLDER}.genome_generate.log" + benchmark: f"{STAR_REF_FOLDER}.genome_generate.benchmark" + conda: "../envs/quant.yaml" + shell: + "(STAR --runMode genomeGenerate --runThreadN {threads} --genomeDir {params.genomedir}" + " --genomeFastaFiles {input.efa} {input.gfa} --sjdbGTFfile {input.gtf}" + " {params.sjoptions} {params.size}) &> {log}" + +rule load_star_genome_firstpass: + input: suffix=f"{STAR_REF_FOLDER}/SA" + output: temp("../results/align/loaded_firstpass") + params: genomedir=lambda w, input: os.path.dirname(input.suffix) + resources: mem_mb = RAM_MB_REQ + log: "../results/align/loaded_firstpass.log" + benchmark: "../results/align/loaded_firstpass.benchmark" + conda: "../envs/quant.yaml" + shell: "(STAR --genomeLoad LoadAndExit --genomeDir {params} && touch {output}) &> {log}" + +rule star_firstpass: + input: + "../results/align/loaded_firstpass", + suffix=f"{STAR_REF_FOLDER}/SA", + fastq="../results/fastq/{sra}.trim.fastq.gz", + output: sj="../results/align/SJ1st/{sra}SJ.out.tab" + threads: 8 + params: + bam="--outSAMtype None", + genomedir=lambda w, input: os.path.dirname(input.suffix), + outfolder=lambda w, output: os.path.dirname(output.sj) + log: "../results/align/SJ1st/{sra}SJ.log" + benchmark: "../results/align/SJ1st/{sra}SJ.benchmark" + conda: "../envs/quant.yaml" + shell: + "(STAR --genomeLoad LoadAndKeep --genomeDir {params.genomedir}" + " --runThreadN {threads} {params.bam} " + " --outFileNamePrefix {params.outfolder}/{wildcards.sra}" + " --readFilesIn <(zcat {input.fastq}) ) &> {log}" + +rule unload_firstpass_genome: + input: + suffix=f"{STAR_REF_FOLDER}/SA", + jj=expand("../results/align/SJ1st/{sra}SJ.out.tab", sra=config['sra']) + output: temp("../results/align/unloaded_firstpass") + params: genomedir=lambda w, input: os.path.dirname(input.suffix) + log: "../results/align/unloaded_firstpass.log" + benchmark: "../results/align/unloaded_firstpass.benchmark" + conda: "../envs/quant.yaml" + shell: + "(STAR --genomeLoad Remove --genomeDir {params.genomedir} && " + "touch {output}) &> {log}" + +rule star_genome_generate_secondpass: + input: + unload="../results/align/unloaded_firstpass", + efa="../resources/ERCC.fa", + gfa=FA, + gtf=f"{GTF}.fix.gtf", + jj=expand("../results/align/SJ1st/{sra}SJ.out.tab", sra=config['sra']) + output: suffix=f"{STAR_REF_FOLDER}SecondPass/SA" + params: + genomedir=lambda w, output: os.path.dirname(output.suffix), + size="--genomeSAindexNbases 12" if FA == TEST_GENOME_FA else "", + sjoptions="--sjdbOverhang 42 --limitSjdbInsertNsj 1200000" # 43bp reads for this experiment + threads: 99 + log: f"{STAR_REF_FOLDER}SecondPass.generate.log" + benchmark: f"{STAR_REF_FOLDER}SecondPass.generate.benchmark" + conda: "../envs/quant.yaml" + shell: + "(STAR --runMode genomeGenerate --runThreadN {threads} --genomeDir {params.genomedir}" + " --genomeFastaFiles {input.efa} {input.gfa} --sjdbGTFfile {input.gtf} {params.sjoptions}" + " --sjdbFileChrStartEnd {input.jj} {params.size}) &> {log}" + +rule load_star_genome_2pass: + input: suffix=f"{STAR_REF_FOLDER}SecondPass/SA" + output: temp("../results/align/loaded_2pass") + params: genomedir=lambda w, input: os.path.dirname(input.suffix), + resources: mem_mb = RAM_MB_REQ + log: "../results/align/loaded_2pass.log" + benchmark: "../results/align/loaded_2pass.benchmark" + conda: "../envs/quant.yaml" + shell: "(STAR --genomeLoad LoadAndExit --genomeDir {params} && touch {output}) &> {log}" + +rule star_2pass: + input: + "../results/align/loaded_2pass", + suffix=f"{STAR_REF_FOLDER}SecondPass/SA", + fastq="../results/fastq/{sra}.trim.fastq.gz" + output: + sj="../results/align/{sra,[A-Z,0-9]+}SJ.out.tab", + bam="../results/align/{sra}Aligned.sortedByCoord.out.bam", + transcript="../results/align/{sra}Aligned.toTranscriptome.out.bam", + log="../results/align/{sra}Log.out", + final="../results/align/{sra}Log.final.out", + progress="../results/align/{sra}Log.progress.out", + wildcard_constraints: sra="[A-Z0-9]+" + threads: 8 + params: + mode="--runMode alignReads --genomeLoad LoadAndKeep", + bam=f"--outSAMtype BAM SortedByCoordinate --outBAMcompression 10 --limitBAMsortRAM {str(RAM_B_REQ)}", + transcripts=f"--quantMode TranscriptomeSAM", + outfolder=lambda w, output: os.path.dirname(output.sj), + genomedir=lambda w, input: os.path.dirname(input.suffix), + log: "../results/align/{sra}Align.log" + benchmark: "../results/align/{sra}Align.benchmark" + conda: "../envs/quant.yaml" + shell: + "(STAR {params.mode} --genomeDir {params.genomedir}" + " --runThreadN {threads} {params.bam} {params.transcripts}" + " --outFileNamePrefix {params.outfolder}/{wildcards.sra}" + " --readFilesIn <(zcat {input.fastq}) ) &> {log}" + +rule unload_2pass_genome: + input: + suffix=f"{STAR_REF_FOLDER}SecondPass/SA", + bam=expand("../results/align/{sra}Aligned.sortedByCoord.out.bam", sra=config['sra']), + output: temp("../results/align/unloaded_2pass") + params: genomedir=lambda w, input: os.path.dirname(input.suffix) + log: "../results/align/unloaded_2pass.log" + benchmark: "../results/align/unloaded_2pass.benchmark" + conda: "../envs/quant.yaml" + shell: + "(STAR --genomeLoad Remove --genomeDir {params.genomedir} && " + "touch {output}) &> {log}" diff --git a/workflow/rules/downloads.smk b/workflow/rules/downloads.smk new file mode 100644 index 0000000..45841fc --- /dev/null +++ b/workflow/rules/downloads.smk @@ -0,0 +1,99 @@ +PROTOCOL = "http" + +rule download_ensembl_references: + output: + gfa=GENOME_FA, + gff3=ENSEMBL_GFF, + gtf=ENSEMBL_GTF, + pfa=f"../resources/ensembl/{REF}.pep.all.fa", + params: + primary=f"{PROTOCOL}://ftp.ensembl.org/pub/release-{ENSEMBL_VERSION}//fasta/{SPECIES_LOWER}/dna/{REF}.dna.primary_assembly.fa.gz", + toplevel=f"{PROTOCOL}://ftp.ensembl.org/pub/release-{ENSEMBL_VERSION}//fasta/{SPECIES_LOWER}/dna/{REF}.dna.toplevel.fa.gz", + gff=f"{PROTOCOL}://ftp.ensembl.org/pub/release-{ENSEMBL_VERSION}/gff3/{SPECIES_LOWER}/{REF}.{ENSEMBL_VERSION}.gff3.gz", + gtf=f"{PROTOCOL}://ftp.ensembl.org/pub/release-{ENSEMBL_VERSION}/gtf/{SPECIES_LOWER}/{REF}.{ENSEMBL_VERSION}.gtf.gz", + pep=f"{PROTOCOL}://ftp.ensembl.org/pub/release-{ENSEMBL_VERSION}//fasta/{SPECIES_LOWER}/pep/{REF}.pep.all.fa.gz", + benchmark: "../resources/ensembl/downloads.benchmark" + log: "../resources/ensembl/downloads.log" + conda: "../envs/downloads.yaml" + shell: + "((wget -O - {params.primary} || wget -O - {params.toplevel}) | gunzip -c - > {output.gfa} && " + "wget -O - {params.gff} | gunzip -c - > {output.gff3} && " + "wget -O - {params.pep} | gunzip -c - > {output.pfa} && " + "wget -O - {params.gtf} | gunzip -c - > {output.gtf}) 2> {log}" + +rule fix_gff3_for_rsem: + '''This script changes descriptive notes in column 4 to "gene" if a gene row, and it also adds ERCCs to the gene model''' + input: ENSEMBL_GFF + output: f"{ENSEMBL_GFF}.fix.gff3" + log: f"{ENSEMBL_GFF}.fix.gff3.log" + benchmark: f"{ENSEMBL_GFF}.fix.gff3.benchmark" + conda: "../envs/downloads.yaml" + shell: "python scripts/fix_gff3_for_rsem.py {input} {output} &> {log}" + +rule fix_gtf_for_rsem: + '''This script changes descriptive notes in column 4 to "gene" if a gene row, and it also adds ERCCs to the gene model''' + input: ENSEMBL_GTF + output: f"{ENSEMBL_GTF}.fix.gtf" + log: f"{ENSEMBL_GTF}.fix.gtf.log" + benchmark: f"{ENSEMBL_GTF}.fix.gtf.benchmark" + conda: "../envs/downloads.yaml" + shell: "python scripts/fix_gff3_for_rsem.py {input} {output} &> {log}" + +rule filter_gff3: + '''For testing, make a smaller gene model''' + input: f"{ENSEMBL_GFF}.fix.gff3" + output: f"{TEST_ENSEMBL_GFF}.fix.gff3" + shell: "grep \"^ERCC\\|^#\\|^20\\|^21\\|^22\" {input} > {output}" + +rule filter_gtf: + '''For testing, make a smaller gene model''' + input: f"{ENSEMBL_GTF}.fix.gtf" + output: f"{TEST_ENSEMBL_GTF}.fix.gtf" + shell: "grep \"^ERCC\\|^#\\|^20\\|^21\\|^22\" {input} > {output}" + +rule filter_fa: + '''For testing, make a smaller genome''' + input: GENOME_FA + output: TEST_GENOME_FA + shell: "python scripts/filter_fasta.py {input} {output}" + +rule prefetch_sras_se: + '''Prefetch SRA from GEO SRA''' + output: temp("../results/sra/{sra}/{sra}.sra") + benchmark: "../results/sra/{sra}/{sra}.benchmark" + log: "../results/sra/{sra}/{sra}.log" + params: outdir=lambda w, output: os.path.dirname(os.path.dirname(output[0])) + conda: "../envs/downloads.yaml" + shell: + "prefetch {wildcards.sra}" + " --output-directory {params.outdir} &> {log}" + +rule split_sras_se: + input: "../results/sra/{sra}/{sra}.sra" + output: "../results/fastq/{sra}.fastq" # independent of pe/se + benchmark: "../results/fastq/{sra}.benchmark" + log: "../results/fastq/{sra}.log" + params: outdir=lambda w, output: os.path.dirname(output[0]) + conda: "../envs/downloads.yaml" + shell: + "fastq-dump -I --outdir {params.outdir} --split-files {input} && " + "mv {params.outdir}/{wildcards.sra}_1.fastq {output} &> {log}" + +rule fastp_sra_se: + '''Trim adapters, read quality filtering, make QC outputs''' + input: "../results/fastq/{sra}.fastq", + output: + fq="../results/fastq/{sra}.trim.fastq.gz", + html="../results/fastq/{sra}.trim.html", + json="../results/fastq/{sra}.trim.json", + threads: 2 + log: "../results/fastq/{sra}.trim.log" + conda: "../envs/downloads.yaml" + params: + quality=20, + title="{sra}" + shell: + "fastp -q {params.quality}" + " -i {input} -o {output.fq}" + " -h {output.html} -j {output.json}" + " -w {threads} -R {params.title} --detect_adapter_for_pe &> {log}" diff --git a/workflow/rules/quant.smk b/workflow/rules/quant.smk new file mode 100644 index 0000000..262ff6e --- /dev/null +++ b/workflow/rules/quant.smk @@ -0,0 +1,98 @@ +rule filter_ercc: + '''Filter the empty ERCC entries''' + input: "../resources/ERCC.fa" + output: "../resources/ERCC.filtered.fa" + log: "../resources/ERCC.filtered.log" + benchmark: "../resources/ERCC.filtered.benchmark" + conda: "../envs/quant.yaml" + shell: "grep -v ERCC_ID {input} > {output} 2> {log}" + +rule rsem_reference: + '''Create an RSEM reference''' + input: + efa="../resources/ERCC.filtered.fa", + gfa=FA, + gtf=f"{GTF}.fix.gtf" + output: + grp=f"{RSEM_REF_FOLDER}RsemReference.grp", + fa=f"{RSEM_REF_FOLDER}RsemReference.transcripts.fa", + suffix=f"{RSEM_REF_FOLDER}SA" + params: prefix=lambda w, output: output.grp[:-4] + threads: 99 # can do fewer without STAR references + log: "../resources/ensembl/prepare-reference.log" + benchmark: "../resources/ensembl/prepare-reference.benchmark" + conda: "../envs/quant.yaml" + shell: + "(rsem-prepare-reference --num-threads {threads} --gtf {input.gtf} " + " --star" # TODO: figure out how to use transcript STAR output + " \"{input.efa}\",\"{input.gfa}\" {params.prefix}) &> {log}" + +rule rsem_calculations: + '''Align to transcripts with STAR and quantify with RSEM''' + input: + grp=f"{RSEM_REF_FOLDER}RsemReference.grp", + suffix=f"{RSEM_REF_FOLDER}SA", + #bam="../results/align/{sra}Aligned.toTranscriptome.out.bam", #single end only + fq="../results/fastq/{sra}.trim.fastq.gz" + output: + "../results/quant/{sra}.isoforms.results", + "../results/quant/{sra}.genes.results", + directory("../results/quant/{sra}.stat"), + params: + prefix=lambda w, input: input.grp[:-4], + results=lambda w, output: os.path.dirname(os.path.dirname(output[0])), + quant=lambda w, output: os.path.dirname(output[0]), + options="--no-bam-output --single-cell-prior" # --bam" + resources: mem_mb=30000 + threads: 8 + log: "../results/quant/{sra}calculate-expression.log" + benchmark: "../results/quant/{sra}calculate-expression.benchmark" + conda: "../envs/quant.yaml" + shell: + "(rsem-calculate-expression --num-threads {threads}" + " --star" + " {params.options} <(zcat {input.fq})" #{input.bam}" + " {params.prefix}" + " {params.quant}/{wildcards.sra}) &> {log}" + +rule make_gene_rsem_dataframe: + '''Take the results from RSEM and put them in a usable dataframe''' + input: + expand("../results/quant/{sra}.genes.results", sra=config['sra']), + gff=f"{GFF}.fix.gff3", + series_matrix="../resources/GSE146773_series_matrix.txt", + srr_lookup="../results/srr_lookup.txt", + output: + counts="../results/quant/Counts.csv", + counts_ercc="../results/quant/Counts.csv.ercc.csv", + names="../results/quant/IdsToNames.csv", + tpms="../results/quant/Tpms.csv", + tpms_ercc="../results/quant/Tpms.csv.ercc.csv", + ids="../results/quant/IsoformToGene.csv.gz", + conda: "../envs/quant.yaml" + log: "../results/quant/Counts.log" + benchmark: "../results/quant/Counts.benchmark" + shell: + "python scripts/make_rsem_dataframe.py genes {input.gff} {input.srr_lookup} {input.series_matrix}" + " {output.counts} {output.tpms} {output.names} {output.ids} &> {log}" + +rule make_isoform_rsem_dataframe: + '''Take the results from RSEM and put them in a usable dataframe''' + input: + expand("../results/quant/{sra}.isoforms.results", sra=config['sra']), + gff=f"{GFF}.fix.gff3", + series_matrix="../resources/GSE146773_series_matrix.txt", + srr_lookup="../results/srr_lookup.txt", + output: + counts="../results/quant/Counts_Isoforms.csv", + counts_ercc="../results/quant/Counts_Isoforms.csv.ercc.csv", + names="../results/quant/IdsToNames_Isoforms.csv", + tpms="../results/quant/Tpms_Isoforms.csv", + tpms_ercc="../results/quant/Tpms_Isoforms.csv.ercc.csv", + ids="../results/quant/IsoformToGene_Isoforms.csv.gz", + conda: "../envs/quant.yaml" + log: "../results/quant/Counts_Isoforms.log" + benchmark: "../results/quant/Counts_Isoforms.benchmark" + shell: + "python scripts/make_rsem_dataframe.py isoforms {input.gff} {input.srr_lookup} {input.series_matrix}" + " {output.counts} {output.tpms} {output.names} {output.ids} &> {log}" diff --git a/workflow/rules/singlecellproteogenomics.smk b/workflow/rules/singlecellproteogenomics.smk new file mode 100644 index 0000000..91ba852 --- /dev/null +++ b/workflow/rules/singlecellproteogenomics.smk @@ -0,0 +1,93 @@ +import os + +INPUTID="149ICTtieYjuKWZoLwRLzimwff0n6eWqw" + +rule SingleCellProteogenomics_copyResults: + input: + quant=[ + "../results/quant/Counts.csv", + "../results/quant/Counts.csv.ercc.csv", + "../results/quant/Tpms.csv", + "../results/quant/Tpms.csv.ercc.csv", + "../results/quant/Counts_Isoforms.csv", + "../results/quant/Counts_Isoforms.csv.ercc.csv", + "../results/quant/Tpms_Isoforms.csv", + "../results/quant/Tpms_Isoforms.csv.ercc.csv", + ], + ids=[ + "../results/quant/IsoformToGene.csv.gz", + "../results/quant/IdsToNames.csv", + "../results/quant/IdsToNames_Isoforms.csv", + ], + velocity=[ + "../results/velocity/a.loom", + "../results/velocity/a.obs_names.csv", + ], + output: directory("../results/newinputs/RNAData/") + conda: "../envs/downloads.yaml" + log: "../results/SingleCellProteogenomics_copyResults.log" + benchmark: "../results/SingleCellProteogenomics_copyResults.benchmark" + shell: + "(mkdir -p {output} && cp {input.quant} {input.ids} {input.velocity} {output}) &> {log}" + +rule downloadInputs: + output: directory("../results/input") + conda: "../../SingleCellProteogenomics/workflow/envs/download.yaml" + log: "../results/SingleCellProteogenomics_downloadInputs.log" + params: outdir=lambda w, output: os.path.dirname(output[0]), + shell: + "(gdown -O {output}.zip" + f" \"https://drive.google.com/uc?export=download&id={INPUTID}\"" + " && unzip -d {params.outdir} {output}.zip) 2> {log}" + +rule ProteinCellCycleClusters: + input: "../results/input/", "../results/newinputs/RNAData/" + output: "../results/output/pickles/mockbulk_phases.npy" + conda: "../../SingleCellProteogenomics/workflow/envs/enviro.yaml" + log: "../results/output/1_ProteinCellCycleClusters.log" + shell: "cd ../results && python ../SingleCellProteogenomics/1_ProteinCellCycleClusters.py &> {log}" + +rule ProteinFucciPsuedotime: + input: "../results/output/pickles/mockbulk_phases.npy" + output: "../results/output/ProteinPseudotimePlotting.csv.gz" + conda: "../../SingleCellProteogenomics/workflow/envs/enviro.yaml" + log: "../results/output/2_ProteinFucciPsuedotime.log" + shell: "cd ../results && python ../SingleCellProteogenomics/2_ProteinFucciPsuedotime.py &> {log}" + +rule RNAFucciPseudotime: + input: "../results/output/ProteinPseudotimePlotting.csv.gz" + output: "../results/output/RNAPseudotimePlotting.csv.gz" + conda: "../../SingleCellProteogenomics/workflow/envs/enviro.yaml" + log: "../results/output/3_RNAFucciPseudotime.log" + shell: "cd ../results && python ../SingleCellProteogenomics/3_RNAFucciPseudotime.py &> {log}" + +rule TemporalDelay: + input: "../results/output/RNAPseudotimePlotting.csv.gz" + output: "../results/output/diff_max_pol.csv" + conda: "../../SingleCellProteogenomics/workflow/envs/enviro.yaml" + log: "../results/output/4_TemporalDelay.log" + shell: "cd ../results && python ../SingleCellProteogenomics/4_TemporalDelay.py &> {log}" + +rule ProteinProperties: + input: "../results/output/diff_max_pol.csv" + output: "../results/output/upstreamKinaseResults.csv" + conda: "../../SingleCellProteogenomics/workflow/envs/enviro.yaml" + log: "../results/output/5_ProteinProperties.log" + shell: "cd ../results && python ../SingleCellProteogenomics/5_ProteinProperties.py &> {log}" + +rule SingleCellProteogenomics_final: + input: + "../results/output/ProteinPseudotimePlotting.csv.gz", + "../results/output/RNAPseudotimePlotting.csv.gz", + "../results/output/upstreamKinaseResults.csv" # prompt full run + output: + protein="../results/final/ProteinPseudotimePlotting.csv.gz", + rna="../results/final/RNAPseudotimePlotting.csv.gz", + conda: "../../SingleCellProteogenomics/workflow/envs/enviro.yaml" + log: "../results/SingleCellProteogenomics_run.log" + benchmark: "../results/SingleCellProteogenomics_run.benchmark" + params: scp_results=lambda w, input: os.path.dirname(input[0]), + threads: 1 + shell: + "(cp {params.scp_results}/ProteinPseudotimePlotting.csv.gz {output.protein} && " + "cp {params.scp_results}/RNAPseudotimePlotting.csv.gz {output.rna}) &> {log}" diff --git a/workflow/rules/velo.smk b/workflow/rules/velo.smk new file mode 100644 index 0000000..7d5c533 --- /dev/null +++ b/workflow/rules/velo.smk @@ -0,0 +1,39 @@ +rule velocity_analysis: + '''Run the velocity analysis''' + input: + unload_genome="../results/align/unloaded_2pass", + gtf=f"{GTF}.fix.gtf", + bams=expand("../results/align/{sra}Aligned.sortedByCoord.out.bam", sra=config['sra']) + output: "../results/velocity/a.loom" + conda: "../envs/velo.yaml" + log: "../results/velocity.log" + benchmark: "../results/velocity.benchmark" + params: + outfolder=lambda w, output: os.path.dirname(output[0]), + sampleid="a" # used this historically, so just keeping it consistent + shell: + "velocyto run-smartseq2 --outputfolder {params.outfolder}" + " --sampleid {params.sampleid} {input.bams} {input.gtf} &> {log}" + +rule srr_lookup: + '''Lookup the SRX accessions for the SRRs''' + output: "../results/srr_lookup.txt" + conda: "../envs/velo.yaml" + log: "../results/srr_lookup.log" + benchmark: "../results/srr_lookup.benchmark" + params: + commandpart1="esearch -db sra -query", + commandpart2="| esummary | xtract -pattern DocumentSummary -element Run@acc Experiment@acc" + shell: "(" + "\n".join(["{params.commandpart1} " + sra + " {params.commandpart2} >> {output}" for sra in config['sra']]) + ") 2> {log}" + +rule make_aobs: + '''Make the observation name file expected''' + input: + series_matrix="../resources/GSE146773_series_matrix.txt", + srr_lookup="../results/srr_lookup.txt", + loom="../results/velocity/a.loom" + output: "../results/velocity/a.obs_names.csv" + log: "../results/velocity/a.obs_names.log" + benchmark: "../results/velocity/a.obs_names.benchmark" + conda: "../envs/velo.yaml" + shell: "python scripts/make_a_obs.py {input.loom} {input.srr_lookup} {input.series_matrix} {output} 2> {log}" diff --git a/scripts/filter_fasta.py b/workflow/scripts/filter_fasta.py similarity index 66% rename from scripts/filter_fasta.py rename to workflow/scripts/filter_fasta.py index bf6be7a..425ccb9 100644 --- a/scripts/filter_fasta.py +++ b/workflow/scripts/filter_fasta.py @@ -1,9 +1,12 @@ +import sys, io from Bio import SeqIO from Bio.Seq import Seq from Bio.SeqRecord import SeqRecord -fasta_sequences = SeqIO.parse(open("ensembl/Homo_sapiens.GRCh38.dna.primary_assembly.fa"),'fasta') +fasta=sys.argv[1] +output=sys.argv[2] +fasta_sequences = SeqIO.parse(open(fasta),'fasta') -with open("ensembl/202122.fa","w") as out: +with open(output,"w") as out: for seq_record in fasta_sequences: if seq_record.id.startswith("20") or seq_record.id.startswith("21") or seq_record.id.startswith("22"): out.write(seq_record.format("fasta")) diff --git a/scripts/fix_gff3_for_rsem.py b/workflow/scripts/fix_gff3_for_rsem.py similarity index 64% rename from scripts/fix_gff3_for_rsem.py rename to workflow/scripts/fix_gff3_for_rsem.py index b7d0f23..e3d3776 100644 --- a/scripts/fix_gff3_for_rsem.py +++ b/workflow/scripts/fix_gff3_for_rsem.py @@ -4,6 +4,7 @@ from Bio.Seq import Seq from Bio.SeqRecord import SeqRecord +isgtf = sys.argv[2].endswith("gtf") with open(sys.argv[2], "w") as outff: with open(sys.argv[1]) as ff: print(f"Reading {sys.argv[1]}") @@ -13,7 +14,7 @@ elif lins[8].startswith("ID=gene:"): lins[2] = "gene" outff.write("\t".join(lins)) - ercc = SeqIO.parse(open("data/ERCC.fa"),'fasta') + ercc = SeqIO.parse(open("../resources/ERCC.fa"),'fasta') for seq_record in ercc: if len(seq_record.seq) > 0: id = seq_record.id.split()[0] @@ -23,6 +24,11 @@ f"ID=transcript:{id};Parent=gene:{id};Name={id};biotype=;transcript_id={id}"]] exon_fields = [str(x) for x in [id, "ERCC", "exon", 1, len(seq_record.seq), ".", "+", ".", f"Parent=transcript:{id};Name={id};exon_id={id}"]] - outff.write("\t".join(gene_fields) + "\n") - outff.write("\t".join(transcript_fields) + "\n") - outff.write("\t".join(exon_fields) + "\n") + exon_fields_gtf = [str(x) for x in [id, "ERCC", "exon", 1, len(seq_record.seq), ".", "+", ".", + f"gene_id \"gene:{id}\"; transcript_id \"transcript:{id}\"; exon_number \"1\"; gene_name \"{id}\"; transcript_name \"{id}\";"]] + if isgtf: + outff.write("\t".join(exon_fields_gtf) + "\n") + else: + outff.write("\t".join(gene_fields) + "\n") + outff.write("\t".join(transcript_fields) + "\n") + outff.write("\t".join(exon_fields) + "\n") diff --git a/workflow/scripts/make_a_obs.py b/workflow/scripts/make_a_obs.py new file mode 100644 index 0000000..ae8921f --- /dev/null +++ b/workflow/scripts/make_a_obs.py @@ -0,0 +1,35 @@ +import scvelo as scv +import sys, io +import numpy as np + +aloomfile = sys.argv[1] +srr_lookup = sys.argv[2] +series_matrix = sys.argv[3] +output = sys.argv[4] + +aloom = scv.read_loom(aloomfile) +aobs = aloom.obs_names + +srr_lookup = dict([x.split()[::-1] for x in open(srr_lookup)]) +series_matrix_lines = open(series_matrix).readlines() +cells, srr, has_srr = [], [], [] +for line in series_matrix_lines: + if line.startswith('!Sample_title'): + cells=[x.strip('"').strip('Single U2OS cell ').strip() for x in line.split('\t')[1:]] + if line.startswith('!Sample_relation') and "SRX" in line: + srrs = [x.split('=')[1][:-1].strip() for x in line.split('\t')[1:]] + has_srr = [x in srr_lookup for x in srrs] + srr = [srr_lookup[x] for x in srrs if x in srr_lookup] + +cells=np.array(cells)[has_srr] # for testing + +if len(cells) != len(srr): + print("Error: series matrix has different cell and srr lengths") + exit(1) + +# Use aobs to look up cell name from cells using the SRR link somehow +srr_to_cell = dict((srr[idx], cells[idx]) for idx in range(len(srr))) +acells = [srr_to_cell[x.split(':')[1].split('Aligned')[0]] for x in aobs] +with open(output, 'w') as output: + output.write('well_plate\n') + output.write('\n'.join(acells)) diff --git a/scripts/make_rsem_dataframe.py b/workflow/scripts/make_rsem_dataframe.py similarity index 67% rename from scripts/make_rsem_dataframe.py rename to workflow/scripts/make_rsem_dataframe.py index 1ba63f2..74a05d7 100644 --- a/scripts/make_rsem_dataframe.py +++ b/workflow/scripts/make_rsem_dataframe.py @@ -4,15 +4,15 @@ import os import glob -USAGE = "python make_rsem_dataframe.py " -if len(sys.argv) != 6: print(USAGE); exit(); -level, gff, outcounts, outtpms, outn = sys.argv[1:] +USAGE = "python make_rsem_dataframe.py " +if len(sys.argv) != 9: print(USAGE); exit(); +level, gff, srr_lookup, series_matrix, outcounts, outtpms, outn, outids = sys.argv[1:] print(f"getting gene names from {gff}") with open(gff) as gffhandle: line_ct = sum([1 for line in gffhandle]) gene_id_to_name, gene_id_to_biotype, gene_id_to_description = {}, {}, {} -transcript_id_to_name, transcript_id_to_biotype, transcript_id_to_description = {}, {}, {} +transcript_id_to_name, transcript_id_to_biotype, transcript_id_to_description, transcript_id_to_gene = {}, {}, {}, {} with open(gff) as gffhandle: for i, line in enumerate(gffhandle): if i % 50000 == 0: print(f"Processed {i} lines out of {line_ct} from {gff}.") @@ -32,14 +32,34 @@ transcript_id_to_name[attributes["ID"][len("transcript:"):]] = attributes["Name"] transcript_id_to_biotype[attributes["ID"][len("transcript:"):]] = attributes["biotype"] if "ID" in attributes and "biotype" in attributes else "" transcript_id_to_description[attributes["ID"][len("transcript:"):]] = attributes["description"] if "ID" in attributes and "description" in attributes else "" + transcript_id_to_gene[attributes["ID"][len("transcript:"):]] = attributes["Parent"][len("gene:"):] if "ID" in attributes and "Parent" in attributes else "" -print(f"globbing output/*/*/*.{level}.results") -files = glob.glob(f"output/*/*/*.{level}.results") + +print(f"globbing ../results/quant/*.{level}.results") +files = glob.glob(f"../results/quant/*.{level}.results") line_ct = sum(1 for line in open(files[0])) +print("Making SRR-to-cell lookup...") +srr_lookup = dict([x.split()[::-1] for x in open(srr_lookup)]) +series_matrix_lines = open(series_matrix).readlines() +cells, srr, has_srr = [], [], [] +for line in series_matrix_lines: + if line.startswith('!Sample_title'): + cells=[x.strip('"').strip('Single U2OS cell ').strip() for x in line.split('\t')[1:]] + if line.startswith('!Sample_relation') and "SRX" in line: + srrs = [x.split('=')[1][:-1].strip() for x in line.split('\t')[1:]] + has_srr = [x in srr_lookup for x in srrs] + srr = [srr_lookup[x] for x in srrs if x in srr_lookup] +cells=np.array(cells)[has_srr] # for testing +if len(cells) != len(srr): + print("Error: series matrix has different cell and srr lengths") + exit(1) +srr_to_cell = dict((srr[idx], cells[idx]) for idx in range(len(srr))) + def get_prefix(file): - return os.path.basename(file).split(".")[0].split("_")[0] + "_" + os.path.dirname(file).split("/")[-2].split("_")[-1] + srr = os.path.basename(file).split(".")[0].split("_")[0] + return srr_to_cell[srr] doUseGene = level.startswith("gene") ids = [line.split('\t')[0].strip("gene:").strip("transcript:") for line in open(files[0])] @@ -71,4 +91,7 @@ def get_prefix(file): print(f"Saving to {outn} ...") np.savetxt(outn, np.column_stack((ids[1:], names, biotypes, description)), delimiter=",", fmt="%s") +print(f"Saving to {outids} ...") +np.savetxt(outids, np.column_stack((list(transcript_id_to_gene.keys()), list(transcript_id_to_gene.values()))), delimiter=",", fmt="%s") + print("Done.")