diff --git a/packages/calcite-components/src/components/list-item/list-item.tsx b/packages/calcite-components/src/components/list-item/list-item.tsx
index 961b39793f7..49afdd5ed6c 100644
--- a/packages/calcite-components/src/components/list-item/list-item.tsx
+++ b/packages/calcite-components/src/components/list-item/list-item.tsx
@@ -12,7 +12,12 @@ import {
   VNode,
   Watch,
 } from "@stencil/core";
-import { getElementDir, slotChangeHasAssignedElement, toAriaBoolean } from "../../utils/dom";
+import {
+  getElementDir,
+  getFirstTabbable,
+  slotChangeHasAssignedElement,
+  toAriaBoolean,
+} from "../../utils/dom";
 import {
   connectInteractive,
   disconnectInteractive,
@@ -344,7 +349,7 @@ export class ListItem
     const focusIndex = focusMap.get(parentListEl);
 
     if (typeof focusIndex === "number") {
-      const cells = [actionsStartEl, contentEl, actionsEndEl].filter(Boolean);
+      const cells = [actionsStartEl, contentEl, actionsEndEl].filter((el) => el && !el.hidden);
       if (cells[focusIndex]) {
         this.focusCell(cells[focusIndex]);
       } else {
@@ -737,7 +742,7 @@ export class ListItem
     const composedPath = event.composedPath();
     const { containerEl, contentEl, actionsStartEl, actionsEndEl, open, openable } = this;
 
-    const cells = [actionsStartEl, contentEl, actionsEndEl].filter(Boolean);
+    const cells = [actionsStartEl, contentEl, actionsEndEl].filter((el) => el && !el.hidden);
     const currentIndex = cells.findIndex((cell) => composedPath.includes(cell));
 
     if (
@@ -790,16 +795,20 @@ export class ListItem
       focusMap.set(parentListEl, null);
     }
 
-    [actionsStartEl, contentEl, actionsEndEl].filter(Boolean).forEach((tableCell, cellIndex) => {
-      const tabIndexAttr = "tabindex";
-      if (tableCell === focusEl) {
-        tableCell.setAttribute(tabIndexAttr, "0");
-        saveFocusIndex && focusMap.set(parentListEl, cellIndex);
-      } else {
-        tableCell.removeAttribute(tabIndexAttr);
-      }
-    });
+    const focusedEl = getFirstTabbable(focusEl);
+
+    [actionsStartEl, contentEl, actionsEndEl]
+      .filter((el) => el && !el.hidden)
+      .forEach((tableCell, cellIndex) => {
+        const tabIndexAttr = "tabindex";
+        if (tableCell === focusEl) {
+          focusEl === focusedEl && tableCell.setAttribute(tabIndexAttr, "0");
+          saveFocusIndex && focusMap.set(parentListEl, cellIndex);
+        } else {
+          tableCell.removeAttribute(tabIndexAttr);
+        }
+      });
 
-    focusEl?.focus();
+    focusedEl?.focus();
   };
 }
diff --git a/packages/calcite-components/src/components/list/list.e2e.ts b/packages/calcite-components/src/components/list/list.e2e.ts
index d875c5bd247..2a581e52dfc 100755
--- a/packages/calcite-components/src/components/list/list.e2e.ts
+++ b/packages/calcite-components/src/components/list/list.e2e.ts
@@ -504,6 +504,70 @@ describe("calcite-list", () => {
 
       expect(await isElementFocused(page, "calcite-filter", { shadowed: true })).toBe(true);
     });
+
+    it("should navigate via ArrowRight and ArrowLeft", async () => {
+      const page = await newE2EPage();
+      await page.setContent(html`
+        <calcite-list>
+          <calcite-list-item id="one" value="one" label="One" description="hello world">
+            <calcite-action
+              appearance="transparent"
+              icon="ellipsis"
+              text="menu"
+              label="menu"
+              slot="actions-end"
+            ></calcite-action>
+            <calcite-list>
+              <calcite-list-item id="two" value="two" label="Two" description="hello world">
+                <calcite-action
+                  appearance="transparent"
+                  icon="ellipsis"
+                  text="menu"
+                  label="menu"
+                  slot="actions-end"
+                ></calcite-action
+              ></calcite-list-item>
+            </calcite-list>
+          </calcite-list-item>
+        </calcite-list>
+      `);
+      await page.waitForChanges();
+      const list = await page.find("calcite-list");
+      await list.callMethod("setFocus");
+      await page.waitForChanges();
+
+      const one = await page.find("#one");
+      expect(await one.getProperty("open")).toBe(false);
+
+      expect(await isElementFocused(page, "#one")).toBe(true);
+
+      await list.press("ArrowRight");
+
+      expect(await isElementFocused(page, "#one")).toBe(true);
+      expect(await one.getProperty("open")).toBe(true);
+
+      await list.press("ArrowRight");
+
+      expect(await isElementFocused(page, `.${CSS.contentContainer}`, { shadowed: true })).toBe(true);
+
+      await list.press("ArrowRight");
+
+      expect(await isElementFocused(page, "calcite-action")).toBe(true);
+
+      await list.press("ArrowLeft");
+
+      expect(await isElementFocused(page, `.${CSS.contentContainer}`, { shadowed: true })).toBe(true);
+
+      await list.press("ArrowLeft");
+
+      expect(await isElementFocused(page, "#one")).toBe(true);
+      expect(await one.getProperty("open")).toBe(true);
+
+      await list.press("ArrowLeft");
+
+      expect(await isElementFocused(page, "#one")).toBe(true);
+      expect(await one.getProperty("open")).toBe(false);
+    });
   });
 
   describe("drag and drop", () => {
diff --git a/packages/calcite-components/src/utils/dom.ts b/packages/calcite-components/src/utils/dom.ts
index c6e6e987eba..d6ab2dec7e6 100644
--- a/packages/calcite-components/src/utils/dom.ts
+++ b/packages/calcite-components/src/utils/dom.ts
@@ -285,16 +285,26 @@ export async function focusElement(el: FocusableElement): Promise<void> {
 }
 
 /**
- * Helper to focus the first tabbable element.
+ * Helper to get the first tabbable element.
  *
  * @param {HTMLElement} element The html element containing tabbable elements.
+ * @returns the first tabbable element.
  */
-export function focusFirstTabbable(element: HTMLElement): void {
+export function getFirstTabbable(element: HTMLElement): HTMLElement {
   if (!element) {
     return;
   }
 
-  (tabbable(element, tabbableOptions)[0] || element).focus();
+  return (tabbable(element, tabbableOptions)[0] ?? element) as HTMLElement;
+}
+
+/**
+ * Helper to focus the first tabbable element.
+ *
+ * @param {HTMLElement} element The html element containing tabbable elements.
+ */
+export function focusFirstTabbable(element: HTMLElement): void {
+  getFirstTabbable(element)?.focus();
 }
 
 interface GetSlottedOptions {