diff --git a/lapis2/src/main/antlr/org/genspectrum/lapis/model/variantqueryparser/VariantQuery.g4 b/lapis2/src/main/antlr/org/genspectrum/lapis/model/variantqueryparser/VariantQuery.g4
index c2b5965f..a550c9a9 100644
--- a/lapis2/src/main/antlr/org/genspectrum/lapis/model/variantqueryparser/VariantQuery.g4
+++ b/lapis2/src/main/antlr/org/genspectrum/lapis/model/variantqueryparser/VariantQuery.g4
@@ -5,6 +5,7 @@ grammar VariantQuery;
 start: expr EOF;
 expr:
   single             # Uni
+  | '!' expr         # Not
   | expr '&' expr    # And
   ;
 
diff --git a/lapis2/src/main/kotlin/org/genspectrum/lapis/model/VariantQueryCustomListener.kt b/lapis2/src/main/kotlin/org/genspectrum/lapis/model/VariantQueryCustomListener.kt
index 50704589..2c7d205b 100644
--- a/lapis2/src/main/kotlin/org/genspectrum/lapis/model/VariantQueryCustomListener.kt
+++ b/lapis2/src/main/kotlin/org/genspectrum/lapis/model/VariantQueryCustomListener.kt
@@ -2,9 +2,11 @@ package org.genspectrum.lapis.model
 
 import VariantQueryBaseListener
 import VariantQueryParser.AndContext
+import VariantQueryParser.NotContext
 import VariantQueryParser.Nucleotide_mutationContext
 import org.antlr.v4.runtime.tree.ParseTreeListener
 import org.genspectrum.lapis.silo.And
+import org.genspectrum.lapis.silo.Not
 import org.genspectrum.lapis.silo.NucleotideSymbolEquals
 import org.genspectrum.lapis.silo.SiloFilterExpression
 
@@ -30,4 +32,9 @@ class VariantQueryCustomListener : VariantQueryBaseListener(), ParseTreeListener
         val children = listOf(expressionStack.removeLast(), expressionStack.removeLast()).reversed()
         expressionStack.addLast(And(children))
     }
+
+    override fun exitNot(ctx: NotContext?) {
+        val child = expressionStack.removeLast()
+        expressionStack.addLast(Not(child))
+    }
 }
diff --git a/lapis2/src/main/kotlin/org/genspectrum/lapis/silo/SiloQuery.kt b/lapis2/src/main/kotlin/org/genspectrum/lapis/silo/SiloQuery.kt
index d0b295a6..18423bf6 100644
--- a/lapis2/src/main/kotlin/org/genspectrum/lapis/silo/SiloQuery.kt
+++ b/lapis2/src/main/kotlin/org/genspectrum/lapis/silo/SiloQuery.kt
@@ -40,3 +40,5 @@ data class DateBetween(val column: String, val from: LocalDate?, val to: LocalDa
 object True : SiloFilterExpression("True")
 
 data class And(val children: List<SiloFilterExpression>) : SiloFilterExpression("And")
+
+data class Not(val child: SiloFilterExpression) : SiloFilterExpression("Not")
diff --git a/lapis2/src/test/kotlin/org/genspectrum/lapis/model/VariantQueryFacadeTest.kt b/lapis2/src/test/kotlin/org/genspectrum/lapis/model/VariantQueryFacadeTest.kt
index 100ef76f..21939199 100644
--- a/lapis2/src/test/kotlin/org/genspectrum/lapis/model/VariantQueryFacadeTest.kt
+++ b/lapis2/src/test/kotlin/org/genspectrum/lapis/model/VariantQueryFacadeTest.kt
@@ -1,6 +1,7 @@
 package org.genspectrum.lapis.model
 
 import org.genspectrum.lapis.silo.And
+import org.genspectrum.lapis.silo.Not
 import org.genspectrum.lapis.silo.NucleotideSymbolEquals
 import org.hamcrest.MatcherAssert
 import org.hamcrest.Matchers
@@ -59,4 +60,14 @@ class VariantQueryFacadeTest {
         )
         MatcherAssert.assertThat(result, Matchers.equalTo(expectedResult))
     }
+
+    @Test
+    fun `given a variant variantQuery with a not expression the map should return the corresponding SiloQuery`() {
+        val variantQuery = "!300G"
+
+        val result = underTest.map(variantQuery)
+
+        val expectedResult = Not(NucleotideSymbolEquals(300, "G"))
+        MatcherAssert.assertThat(result, Matchers.equalTo(expectedResult))
+    }
 }
diff --git a/lapis2/src/test/kotlin/org/genspectrum/lapis/silo/SiloQueryTest.kt b/lapis2/src/test/kotlin/org/genspectrum/lapis/silo/SiloQueryTest.kt
index 861896d1..f122c77d 100644
--- a/lapis2/src/test/kotlin/org/genspectrum/lapis/silo/SiloQueryTest.kt
+++ b/lapis2/src/test/kotlin/org/genspectrum/lapis/silo/SiloQueryTest.kt
@@ -188,6 +188,19 @@ class SiloQueryTest {
                 }
                 """,
             ),
+            Arguments.of(
+                Not(StringEquals("theColumn", "theValue")),
+                """
+                {
+                    "type": "Not",
+                    "child": {
+                        "type": "StringEquals",
+                        "column": "theColumn",
+                        "value": "theValue"
+                    }
+                }
+                """,
+            ),
         )
     }
 }