diff --git a/NEWS.md b/NEWS.md
index fb2ab269fc2c4..d3c777ca421a1 100644
--- a/NEWS.md
+++ b/NEWS.md
@@ -1078,6 +1078,8 @@ Deprecated or removed
   * `contains` has been deprecated in favor of a more general `occursin` function, which
     takes its arguments in reverse order from `contains` ([#26283]).
 
+  * `Regex` objects are no longer callable. Use `occursin` instead ([#26283]).
+
   * The methods of `range` based on positional arguments have been deprecated in favor of
     keyword arguments ([#25896]).
 
diff --git a/base/deprecated.jl b/base/deprecated.jl
index cc6d80736868d..4cf1a50abf81f 100644
--- a/base/deprecated.jl
+++ b/base/deprecated.jl
@@ -1470,6 +1470,10 @@ end
 # PR #26283
 @deprecate contains(haystack, needle) occursin(needle, haystack)
 @deprecate contains(s::AbstractString, r::Regex, offset::Integer) occursin(r, s, offset=offset)
+function (r::Regex)(s)
+    depwarn("`(r::Regex)(s)` is deprecated, use `occursin(r, s)` instead.", :Regex)
+    occursin(r, s)
+end
 
 # Issue #25786
 @deprecate_binding DevNull devnull
diff --git a/base/regex.jl b/base/regex.jl
index d05a8275a9792..cec07bb3ea5d8 100644
--- a/base/regex.jl
+++ b/base/regex.jl
@@ -153,8 +153,6 @@ function occursin(r::Regex, s::SubString; offset::Integer=0)
                      r.match_data)
 end
 
-(r::Regex)(s) = isfound(r, s)
-
 """
     match(r::Regex, s::AbstractString[, idx::Integer[, addopts]])