diff --git a/basicsynbio/parts_linkers/BASIC_SEVA_collection_v10.gb b/basicsynbio/parts_linkers/BASIC_SEVA_collection_v10.gb index f31aa0e..51dc52b 100644 --- a/basicsynbio/parts_linkers/BASIC_SEVA_collection_v10.gb +++ b/basicsynbio/parts_linkers/BASIC_SEVA_collection_v10.gb @@ -1,8 +1,8 @@ -LOCUS BASIC_SEVA_15.10 5933 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_15a.10 5933 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Ampicillin resistance marker and - RSF1010 origin of replication.. -ACCESSION BASIC_SEVA_15 -VERSION BASIC_SEVA_15.10 + RSF1010 origin of replication. +ACCESSION f695f7983bc926fe22312ceea133ece8 +VERSION f695f7983bc926fe22312ceea133ece8 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -16,8 +16,8 @@ FEATURES Location/Qualifiers /label="SEVA_Ap" misc_feature 1244..1296 /label="BSEVA_L1" - misc_feature 1305..4978 - /label="SEVA_RSF101" + misc_feature 1308..4978 + /label="SEVA_RSF1010(x5ax)" misc_feature 4993..5097 /label="SEVA_T1" misc_feature 5109..5163 @@ -50,61 +50,61 @@ ORIGIN 1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta 1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta 1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg - 1261 tctcgtacaa ttctgaggtc cacacagata gtgtccggcc ggcctcagcc tgccgccttg - 1321 ggccgggtga tgtcgtactt gcccgccgcg aactcggtta ccgtccagcc cagcgcgacc - 1381 agctccggca acgcctcgcg cacccgctgg cggcgcttgc gcatggtcga accactggcc - 1441 tctgacggcc agacatagcc gcacaaggta tctatggaag ccttgccggt tttgccgggg - 1501 tcgatccagc cacacagccg ctggtgcagc aggcgggcgg tttcgctgtc cagcgcccgc - 1561 acctcgtcca tgctgatgcg cacatgctgg ccgccaccca tgacggcctg cgcgatcaag - 1621 gggttcaggg ccacgtacag gcgcccgtcc gcctcgtcgc tggcgtactc cgacagcagc - 1681 cgaaacccct gccgcttgcg gccattctgg gcgatgatgg ataccttcca aaggcgctcg - 1741 atgcagtcct gtatgtgctt gagcgcccca ccactatcga cctctgcccc gatttccttt - 1801 gccagcgccc gatagctacc tttgaccaca tggcattcag cggtgacggc ctcccacttg - 1861 ggttccagga acagccggag ctgccgtccg ccttcggtct tgggttccgg gccaagcact - 1921 aggccattag gcccagccat ggccaccagc ccttgcagga tgcgcagatc atcagcgccc - 1981 agcggctccg ggccgctgaa ctcgatccgc ttgccgtcgc cgtagtcata cgtcacgtcc - 2041 agcttgctgc gcttgcgctc gccccgcttg agggcacgga acaggccggg ggccagacag - 2101 tgcgccgggt cgtgccggac gtggctgagg ctgtgcttgt tcttaggctt caccacgggg - 2161 cacccccttg ctcttgcgct gcctctccag cacggcgggc ttgagcaccc cgccgtcatg - 2221 ccgcctgaac caccgatcag cgaacggtgc gccatagttg gccttgctca caccgaagcg - 2281 gacgaagaac cggcgctggt cgtcgtccac accccattcc tcggcctcgg cgctggtcat - 2341 gctcgacagg taggactgcc agcggatgtt atcgaccagt accgagctgc cccggctggc - 2401 ctgctgctgg tcgcctgcgc ccatcatggc cgcgcccttg ctggcatggt gcaggaacac - 2461 gatagagcac ccggtatcgg cggcgatggc ctccatgcga ccgatgacct gggccatggg - 2521 gccgctggcg ttttcttcct cgatgtggaa ccggcgcagc gtgtccagca ccatcaggcg - 2581 gcggccctcg gcggcgcgct tgaggccgtc gaaccactcc ggggccatga tgttgggcag - 2641 gctgccgatc agcggctgga tcagcaggcc gtcagccacg gcttgccgtt cctcggcgct - 2701 gaggtgcgcc ccaagggcgt gcaggcggtg atgaatggcg gtgggcgggt cttcggcggg - 2761 caggtagatc accgggccgg tgggcagttc gcccacctcc agcagatccg gcccgcctgc - 2821 aatctgtgcg gccagttgca gggccagcat ggatttaccg gcaccaccgg gcgacaccag - 2881 cgccccgacc gtaccggcca ccatgttggg caaaacgtag tccagcggtg gcggcgctgc - 2941 tgcgaacgcc tccagaatat tgataggctt atgggtagcc attgattgcc tcctttgcag - 3001 gcagttggtg gttaggcgct ggcggggtca ctacccccgc cctgcgccgc tctgagttct - 3061 tccaggcact cgcgcagcgc ctcgtattcg tcgtcggtca gccagaactt gcgctgacgc - 3121 atccctttgg ccttcatgcg ctcggcatat cgcgcttggc gtacagcgtc agggctggcc - 3181 agcaggtcgc cggtctgctt gtccttttgg tctttcatat cagtcaccga gaaacttgcc - 3241 ggggccgaaa ggcttgtctt cgcggaacaa ggacaaggtg cagccgtcaa ggttaaggct - 3301 ggccatatca gcgactgaaa agcggccagc ctcggccttg tttgacgtat aaccaaagcc - 3361 accgggcaac caatagccct tgtcactttt gatcaggtag accgaccctg aagcgctttt - 3421 ttcgtattcc ataaaacccc cttctgtgcg tgagtactca tagtataaca ggcgtgagta - 3481 ccaacgcaag cactacatgc tgaaatctgg cccgcccctg tccatgcctc gctggcgggg - 3541 tgccggtgcc cgtgccagct cggcccgcgc aagctggacg ctgggcagac ccatgacctt - 3601 gctgacggtg cgctcgatgt aatccgcttc gtggccgggc ttgcgctctg ccagcgctgg - 3661 gctggcctcg gccatggcct tgccgatttc ctcggcactg cggccccggc tggccagctt - 3721 ctgcgcggcg ataaagtcgc acttgctgag gtcatcaccg aagcgcttga ccagcccggc - 3781 catctcgctg cggtactcgt ccagcgccgt gcgccggtgg cggctaagct gccgctcggg - 3841 cagttcgagg ctggccagcc tgcgggcctt ctcctgctgc cgctgggcct gctcgatctg - 3901 ctggccagcc tgctgcacca gcgccgggcc agcggtggcg gtcttgccct tggattcacg - 3961 cagcagcacc cacggctgat aaccggcgcg ggtggtgtgc ttgtccttgc ggttggtgaa - 4021 gcccgccaag cggccatagt ggcggctgtc ggcgctggcc gggtcggcgt cgtactcgct - 4081 ggccagcgtc cgggcaatct gcccccgaag ttcaccgcct gcggcgtcgg ccaccttgac - 4141 ccatgcctga tagttcttcg ggctggtttc cactaccagg gcaggctccc ggccctcggc - 4201 tttcatgtca tccaggtcaa actcgctgag gtcgtccacc agcaccagac catgccgctc - 4261 ctgctcggcg ggcctgatat acacgtcatt gccctgggca ttcatccgct tgagccatgg - 4321 cgtgttctgg agcacttcgg cggctgacca ttcccggttc atcatctggc cggtggtggc - 4381 gtccctgacg ccgatatcga agcgctcaca gcccatggcc ttgagctgtc ggcctatggc - 4441 ctgcaaagtc ctgtcgttct tcatcgggcc accaagcgat tcccacacat tatacgagcc - 4501 ggaagcataa agtgtaaagc ctagatccga aggatgagcc gggctgaatg atcgaccgag + 1261 tctcgtacaa ttctgaggtc cacacagata gtgtccgtag gccggcctca gcctgccgcc + 1321 ttgggccggg tgatgtcgta cttgcccgcc gcgaactcgg ttaccgtcca gcccagcgcg + 1381 accagctccg gcaacgcctc gcgcacccgc tggcggcgct tgcgcatggt cgaaccactg + 1441 gcctctgacg gccagacata gccgcacaag gtatctatgg aagccttgcc ggttttgccg + 1501 gggtcgatcc agccacacag ccgctggtgc agcaggcggg cggtttcgct gtccagcgcc + 1561 cgcacctcgt ccatgctgat gcgcacatgc tggccgccac ccatgacggc ctgcgcgatc + 1621 aaggggttca gggccacgta caggcgcccg tccgcctcgt cgctggcgta ctccgacagc + 1681 agccgaaacc cctgccgctt gcggccattc tgggcgatga tggatacctt ccaaaggcgc + 1741 tcgatgcagt cctgtatgtg cttgagcgcc ccaccactat cgacctctgc cccgatttcc + 1801 tttgccagcg cccgatagct acctttgacc accatggcat cagcggtgac ggcctcccac + 1861 ttgggttcca ggaacagccg gagctgccgt ccgccttcgg tcttgggttc cgggccaagc + 1921 actaggccat taggcccagc catggccacc agcccttgca ggatgcgcag atcatcagcg + 1981 cccagcggct ccgggccgct gaactcgatc cgcttgccgt cgccgtagtc atacgtcacg + 2041 tccagcttgc tgcgcttgcg ctcgccccgc ttgagggcac ggaacaggcc gggggccaga + 2101 cagtgcgccg ggtcgtgccg gacgtggctg aggctgtgct tgttcttagg cttcaccacg + 2161 gggcaccccc ttgctcttgc gctgcctctc cagcacggcg ggcttgagca ccccgccgtc + 2221 atgccgcctg aaccaccgat cagcgaacgg tgcgccatag ttggccttgc tcacaccgaa + 2281 gcggacgaag aaccggcgct ggtcgtcgtc cacaccccat tcctcggcct cggcgctggt + 2341 catgctcgac aggtaggact gccagcggat gttatcgacc agtaccgagc tgccccggct + 2401 ggcctgctgc tggtcgcctg cgcccatcat ggccgcgccc ttgctggcat ggtgcaggaa + 2461 cacgatagag cacccggtat cggcggcgat ggcctccatg cgaccgatga cctgggccat + 2521 ggggccgctg gcgttttctt cctcgatgtg gaaccggcgc agcgtgtcca gcaccatcag + 2581 gcggcggccc tcggcggcgc gcttgaggcc gtcgaaccac tccggggcca tgatgttggg + 2641 caggctgccg atcagcggct ggatcagcag gccgtcagcc acggcttgcc gttcctcggc + 2701 gctgaggtgc gccccaaggg cgtgcaggcg gtgatgaatg gcggtgggcg ggtcttcggc + 2761 gggcaggtag atcaccgggc cggtgggcag ttcgcccacc tccagcagat ccggcccgcc + 2821 tgcaatctgt gcggccagtt gcagggccag catggattta ccggcaccac cgggcgacac + 2881 cagcgccccg accgtaccgg ccaccatgtt gggcaaaacg tagtccagcg gtggcggcgc + 2941 tgctgcgaac gcctccagaa tattgatagg cttatgggta gccattgatt gcctcctttg + 3001 caggcagttg gtggttaggc gctggcgggg tcactacccc cgccctgcgc cgctctgagt + 3061 tcttccaggc actcgcgcag cgcctcgtat tcgtcgtcgg tcagccagaa cttgcgctga + 3121 cgcatccctt tggccttcat gcgctcggca tatcgcgctt ggcgtacagc gtcagggctg + 3181 gccagcaggt cgccggtctg cttgtccttt tggtctttca tatcagtcac cgagaaactt + 3241 gccggggccg aaaggcttgt cttcgcggaa caaggacaag gtgcagccgt caaggttaag + 3301 gctggccata tcagcgactg aaaagcggcc agcctcggcc ttgtttgacg tataaccaaa + 3361 gccaccgggc aaccaatagc ccttgtcact tttgatcagg tagaccgacc ctgaagcgct + 3421 tttttcgtat tccataaaac ccccttctgt gcgtgagtac tcatagtata acaggcgtga + 3481 gtaccaacgc aagcactaca tgctgaaatc tggcccgccc ctgtccatgc ctcgctggcg + 3541 gggtgccggt gcccgtgcca gctcggcccg cgcaagctgg acgctgggca gacccatgac + 3601 cttgctgacg gtgcgctcga tgtaatccgc ttcgtggccg ggcttgcgct ctgccagcgc + 3661 tgggctggcc tcggccatgg ccttgccgat ttcctcggca ctgcggcccc ggctggccag + 3721 cttctgcgcg gcgataaagt cgcacttgct gaggtcatca ccgaagcgct tgaccagccc + 3781 ggccatctcg ctgcggtact cgtccagcgc cgtgcgccgg tggcggctaa gctgccgctc + 3841 gggcagttcg aggctggcca gcctgcgggc cttctcctgc tgccgctggg cctgctcgat + 3901 ctgctggcca gcctgctgca ccagcgccgg gccagcggtg gcggtcttgc ccttggattc + 3961 acgcagcagc acccacggct gataaccggc gcgggtggtg tgcttgtcct tgcggttggt + 4021 gaagcccgcc aagcggccat agtggcggct gtcggcgctg gccgggtcgg cgtcgtactc + 4081 gctggccagc gtccgggcaa tctgcccccg aagttcaccg cctgcggcgt cggccacctt + 4141 gacccatgcc tgatagttct tcgggctggt ttccactacc agggcaggct cccggccctc + 4201 ggctttcatg tcatccaggt caaactcgct gaggtcgtcc accagcacca gaccatgccg + 4261 ctcctgctcg gcgggcctga tatacacgtc attgccctgg gcattcatcc gcttgagcca + 4321 tggcgtgttc tggagcactt cggcggctga ccattcccgg ttcatcatct ggccggtggt + 4381 ggcgtccctg acgccgatat cgaagcgctc acagcccatg gccttgagct gtcggcctat + 4441 ggcctgcaaa gtcctgtcgt tcttcatcgg gccaccaagc gattcccaca cattatacga + 4501 gccggaagca taaagtgtag ctagatccga aggatgagcc gggctgaatg atcgaccgag 4561 acaggccctg cggggctgca cacgcgcccc cacccttcgg gtagggggaa aggccgctaa 4621 agcggctaaa agcgctccag cgtatttctg cggggtttgg tgtggggttt agcgggcttt 4681 gcccgccttt ccccctgccg cgcagcggtg gggcggtgtg tagcctagcg cagcgaatag @@ -129,11 +129,11 @@ ORIGIN 5821 aactggatat taccagccac aacgaggatt ataccgtggt tgagcaatat gagcgtagcg 5881 agggtcgcca cagcaccggc ggcatggacg aactgtataa gggatcctaa taa // -LOCUS BASIC_SEVA_16.10 2991 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_16.10 2991 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Ampicillin resistance marker and p15A - origin of replication.. -ACCESSION BASIC_SEVA_16 -VERSION BASIC_SEVA_16.10 + origin of replication. +ACCESSION 6568bcea77ad3ed95be9cf305b393522 +VERSION 6568bcea77ad3ed95be9cf305b393522 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -211,11 +211,11 @@ ORIGIN 2881 ctggatatta ccagccacaa cgaggattat accgtggttg agcaatatga gcgtagcgag 2941 ggtcgccaca gcaccggcgg catggacgaa ctgtataagg gatcctaata a // -LOCUS BASIC_SEVA_17.10 3915 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_17.10 3915 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Ampicillin resistance marker and pSC101 - origin of replication.. -ACCESSION BASIC_SEVA_17 -VERSION BASIC_SEVA_17.10 + origin of replication. +ACCESSION 50e0aa1b55ef02db0cf62a84c4ecb719 +VERSION 50e0aa1b55ef02db0cf62a84c4ecb719 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -309,11 +309,11 @@ ORIGIN 3841 gttgagcaat atgagcgtag cgagggtcgc cacagcaccg gcggcatgga cgaactgtat 3901 aagggatcct aataa // -LOCUS BASIC_SEVA_17_TS.10 3984 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_19.10 3644 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Ampicillin resistance marker and - temperature sensitive pSC101 origin of replication.. -ACCESSION BASIC_SEVA_17_TS -VERSION BASIC_SEVA_17_TS.10 + pBR322-ROP origin of replication. +ACCESSION 314fdd9dca836243b4181ccd287e2dc0 +VERSION 314fdd9dca836243b4181ccd287e2dc0 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -327,118 +327,17 @@ FEATURES Location/Qualifiers /label="SEVA_Ap" misc_feature 1244..1296 /label="BSEVA_L1" - CDS complement(1358..2308) - /label="Rep101(Ts)" - rep_origin 2356..2578 - /label="pSC101 ori" - misc_feature 2848..2952 - /label="SEVA_T1" - misc_feature 2964..3018 - /label="LMS" - misc_feature 3111..3145 - /label="J23119" - misc_feature 3263..3270 - /label="RBS-A12" - CDS 3277..3981 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttaa attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt - 241 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc - 301 ttcaataata ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc - 361 cgttttttgc ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga - 421 aagatgcgga agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg - 481 gcaaaattct ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag - 541 tgctgctgtg cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc - 601 gtattcatta tagccagaac gatctggtgg aatatagccc ggtgaccgaa aaacatctga - 661 ccgatggcat gaccgtgcgt gaactgtgca gcgcggcgat taccatgagc gataacaccg - 721 cggcgaacct gctgctgacg accattggcg gtccgaaaga actgaccgcg tttctgcata - 781 acatgggcga tcatgtgacc cgtctggatc gttgggaacc ggaactgaac gaagcgattc - 841 cgaacgatga acgtgatacc accatgccgg cagcaatggc gaccaccctg cgtaaactgc - 901 tgacgggtga gctgctgacc ctggcaagcc gccagcaact gattgattgg atggaagcgg - 961 ataaagtggc gggtccgctg ctgcgtagcg cgctgccggc tggctggttt attgcggata - 1021 aaagcggtgc gggcgaacgt ggcagccgtg gcattattgc ggcgctgggc ccggatggta - 1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta - 1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta - 1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg - 1261 tctcgtacaa ttctgaggtc cacacagata gtgtcctgtt ctacttttgt ttgttagtct - 1321 tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg tatttagcca - 1381 gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat - 1441 catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg - 1501 cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa tctgatgtaa - 1561 tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga - 1621 gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct - 1681 tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa - 1741 cccattggtt aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa - 1801 attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta - 1861 ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat - 1921 tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta - 1981 attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc tcaaagcctt - 2041 taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta - 2101 atacaccata agcattttcc ctactgatgt tcatcatctg aacgtattgg ttataagtga - 2161 acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac - 2221 agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat - 2281 ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac - 2341 tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt tgagttgtgg - 2401 gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg - 2461 taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata - 2521 gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac - 2581 tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac - 2641 aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg gttgcggccg - 2701 caatcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg - 2761 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg - 2821 gcactacagg cgcggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag - 2881 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt - 2941 ttatttgatg cctttaatta aggctcggga gacctatcgg taataacagt ccaatctggt - 3001 gtaacttcgg aatcgtcccc aattattgaa cacccttcgg ggtgtttttt tgtttctggt - 3061 ctaccatctc gttgtgataa tagacctgaa gtgcctactc tggaaaatct ttgacagcta - 3121 gctcagtcct aggtataatg ctagcagctg tcaccggatg tgctttccgg tctgatgagt - 3181 ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa ggctcgctac gagaagattg - 3241 ttactttcgc agcgttatta tctaaggagg tagtccatgg ttagcaaagg cgaggcggtt - 3301 atcaaggagt ttatgcgttt taaggttcac atggagggta gcatgaatgg tcacgagttc - 3361 gagatcgagg gtgaaggcga gggtcgtccg tacgaaggca cccagaccgc gaagctgaaa - 3421 gtgaccaagg gtggcccgct gccgttcagc tgggacatcc tgagcccgca gttcatgtat - 3481 ggcagccgtg cgtttaccaa acacccggcg gacattccgg attactataa gcaaagcttc - 3541 ccggaaggtt ttaaatggga gcgtgttatg aacttcgaag atggtggcgc ggtgaccgtt - 3601 acccaggaca ccagcctgga ggatggcacc ctgatttaca aggtgaaact gcgtggcacc - 3661 aactttccgc cggatggtcc ggttatgcag aagaaaacga tgggttggga agcgagcacc - 3721 gagcgtctgt atccggaaga tggcgtgctg aagggtgata tcaaaatggc gctgcgtctg - 3781 aaggacggtg gccgttacct ggcggatttt aagaccacct ataaagcgaa gaaaccggtg - 3841 caaatgccgg gtgcgtacaa cgttgaccgt aaactggata ttaccagcca caacgaggat - 3901 tataccgtgg ttgagcaata tgagcgtagc gagggtcgcc acagcaccgg cggcatggac - 3961 gaactgtata agggatccta ataa -// -LOCUS BASIC_SEVA_18.10 3188 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Ampicillin resistance marker and pUC - origin of replication.. -ACCESSION BASIC_SEVA_18 -VERSION BASIC_SEVA_18.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 193..1231 - /label="SEVA_Ap" - misc_feature 1244..1296 - /label="BSEVA_L1" - misc_feature 1305..2233 - /label="SEVA_pUC" - misc_feature 2248..2352 + misc_feature 1305..2689 + /label="SEVA_pBR322-ROP" + misc_feature 2704..2808 /label="SEVA_T1" - misc_feature 2364..2418 + misc_feature 2820..2874 /label="LMS" - misc_feature 2419..2453 + misc_feature 2875..2909 /label="J23106" - misc_feature 2462..2473 + misc_feature 2918..2929 /label="B0034" - CDS 2481..3185 + CDS 2937..3641 /label="mScarlet" ORIGIN 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact @@ -465,42 +364,49 @@ ORIGIN 1261 tctcgtacaa ttctgaggtc cacacagata gtgtccggcc ggccccgtag aaaagatcaa 1321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 1381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt - 1441 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg + 1441 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg 1501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 1561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 1621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga - 1681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctttgagaaa gcgccacgct + 1681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 1741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 1801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 1861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 1921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 1981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 2041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga - 2101 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca - 2161 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct - 2221 cactcattag gcaggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag - 2281 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt - 2341 ttatttgatg cctttaatta aggctcggga gacctatcgg taataacagt ccaatctggt - 2401 gtaacttcgg aatcgtcctt tacggctagc tcagtcctag gtatagtgct agctactagt - 2461 gaaagaggag aaatactagt atggttagca aaggcgaggc ggttatcaag gagtttatgc - 2521 gttttaaggt tcacatggag ggtagcatga atggtcacga gttcgagatc gagggtgaag - 2581 gcgagggtcg tccgtacgaa ggcacccaga ccgcgaagct gaaagtgacc aagggtggcc - 2641 cgctgccgtt cagctgggac atcctgagcc cgcagttcat gtatggcagc cgtgcgttta - 2701 ccaaacaccc ggcggacatt ccggattact ataagcaaag cttcccggaa ggttttaaat - 2761 gggagcgtgt tatgaacttc gaagatggtg gcgcggtgac cgttacccag gacaccagcc - 2821 tggaggatgg caccctgatt tacaaggtga aactgcgtgg caccaacttt ccgccggatg - 2881 gtccggttat gcagaagaaa acgatgggtt gggaagcgag caccgagcgt ctgtatccgg - 2941 aagatggcgt gctgaagggt gatatcaaaa tggcgctgcg tctgaaggac ggtggccgtt - 3001 acctggcgga ttttaagacc acctataaag cgaagaaacc ggtgcaaatg ccgggtgcgt - 3061 acaacgttga ccgtaaactg gatattacca gccacaacga ggattatacc gtggttgagc - 3121 aatatgagcg tagcgagggt cgccacagca ccggcggcat ggacgaactg tataagggat - 3181 cctaataa + 2101 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg + 2161 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg + 2221 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga + 2281 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc + 2341 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca + 2401 tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg + 2461 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt + 2521 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg + 2581 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg + 2641 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcgggg gcgcgcccag + 2701 ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa + 2761 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctt taattaaggc + 2821 tcgggagacc tatcggtaat aacagtccaa tctggtgtaa cttcggaatc gtcctttacg + 2881 gctagctcag tcctaggtat agtgctagct actagtgaaa gaggagaaat actagtatgg + 2941 ttagcaaagg cgaggcggtt atcaaggagt ttatgcgttt taaggttcac atggagggta + 3001 gcatgaatgg tcacgagttc gagatcgagg gtgaaggcga gggtcgtccg tacgaaggca + 3061 cccagaccgc gaagctgaaa gtgaccaagg gtggcccgct gccgttcagc tgggacatcc + 3121 tgagcccgca gttcatgtat ggcagccgtg cgtttaccaa acacccggcg gacattccgg + 3181 attactataa gcaaagcttc ccggaaggtt ttaaatggga gcgtgttatg aacttcgaag + 3241 atggtggcgc ggtgaccgtt acccaggaca ccagcctgga ggatggcacc ctgatttaca + 3301 aggtgaaact gcgtggcacc aactttccgc cggatggtcc ggttatgcag aagaaaacga + 3361 tgggttggga agcgagcacc gagcgtctgt atccggaaga tggcgtgctg aagggtgata + 3421 tcaaaatggc gctgcgtctg aaggacggtg gccgttacct ggcggatttt aagaccacct + 3481 ataaagcgaa gaaaccggtg caaatgccgg gtgcgtacaa cgttgaccgt aaactggata + 3541 ttaccagcca caacgaggat tataccgtgg ttgagcaata tgagcgtagc gagggtcgcc + 3601 acagcaccgg cggcatggac gaactgtata agggatccta ataa // -LOCUS BASIC_SEVA_19.10 3644 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_17_pKD46.10 3984 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Ampicillin resistance marker and - pBR322-ROP origin of replication.. -ACCESSION BASIC_SEVA_19 -VERSION BASIC_SEVA_19.10 + temperature sensitive pSC101 origin of replication. +ACCESSION c2ce6224a5d3a0001db46ce89a840642 +VERSION c2ce6224a5d3a0001db46ce89a840642 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -514,17 +420,19 @@ FEATURES Location/Qualifiers /label="SEVA_Ap" misc_feature 1244..1296 /label="BSEVA_L1" - misc_feature 1305..2689 - /label="SEVA_pBR322-ROP" - misc_feature 2704..2808 + CDS 1358..2308 + /label="Rep101(pKD46)" + rep_origin 2356..2578 + /label="pSC101 ori" + misc_feature 2848..2952 /label="SEVA_T1" - misc_feature 2820..2874 + misc_feature 2964..3018 /label="LMS" - misc_feature 2875..2909 + misc_feature 3111..3145 /label="J23119" - misc_feature 2918..2929 - /label="B0034" - CDS 2937..3641 + misc_feature 3263..3270 + /label="RBS-A12" + CDS 3277..3981 /label="mScarlet" ORIGIN 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact @@ -548,52 +456,58 @@ ORIGIN 1081 aaccgagccg tattgtggtg atttatacca ccggcagcca ggcgacgatg gatgaacgta 1141 accgtcagat tgcggaaatt ggcgcgagcc tgattaaaca ttggtaaacc gatacaatta 1201 aaggctcctt ttggagcctt tttttttgga cgacccttgt cggctcggcc cacttgtgtg - 1261 tctcgtacaa ttctgaggtc cacacagata gtgtccggcc ggccccgtag aaaagatcaa - 1321 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc - 1381 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt - 1441 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg - 1501 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc - 1561 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt - 1621 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga - 1681 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct - 1741 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg - 1801 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca - 1861 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa - 1921 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt - 1981 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga - 2041 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga - 2101 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg - 2161 cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg - 2221 ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga - 2281 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc - 2341 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca - 2401 tcagcgtggt cgtgcagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg - 2461 agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt - 2521 ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg - 2581 ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg - 2641 ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcgggg gcgcgcccag - 2701 ctgtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa - 2761 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctt taattaaggc - 2821 tcgggagacc tatcggtaat aacagtccaa tctggtgtaa cttcggaatc gtccttgaca - 2881 gctagctcag tcctaggtat aatgctagct actagtgaaa gaggagaaat actagtatgg - 2941 ttagcaaagg cgaggcggtt atcaaggagt ttatgcgttt taaggttcac atggagggta - 3001 gcatgaatgg tcacgagttc gagatcgagg gtgaaggcga gggtcgtccg tacgaaggca - 3061 cccagaccgc gaagctgaaa gtgaccaagg gtggcccgct gccgttcagc tgggacatcc - 3121 tgagcccgca gttcatgtat ggcagccgtg cgtttaccaa acacccggcg gacattccgg - 3181 attactataa gcaaagcttc ccggaaggtt ttaaatggga gcgtgttatg aacttcgaag - 3241 atggtggcgc ggtgaccgtt acccaggaca ccagcctgga ggatggcacc ctgatttaca - 3301 aggtgaaact gcgtggcacc aactttccgc cggatggtcc ggttatgcag aagaaaacga - 3361 tgggttggga agcgagcacc gagcgtctgt atccggaaga tggcgtgctg aagggtgata - 3421 tcaaaatggc gctgcgtctg aaggacggtg gccgttacct ggcggatttt aagaccacct - 3481 ataaagcgaa gaaaccggtg caaatgccgg gtgcgtacaa cgttgaccgt aaactggata - 3541 ttaccagcca caacgaggat tataccgtgg ttgagcaata tgagcgtagc gagggtcgcc - 3601 acagcaccgg cggcatggac gaactgtata agggatccta ataa + 1261 tctcgtacaa ttctgaggtc cacacagata gtgtcctgtt ctacttttgt ttgttagtct + 1321 tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg tatttagcca + 1381 gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat + 1441 catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc tgaatttttg + 1501 cagttaaagc atcgtgtagt gtttttctta gtccgttacg taggtaggaa tctgatgtaa + 1561 tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt tcggttacga + 1621 gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg cggcctcgct + 1681 tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt ggtttcaaaa + 1741 cccattggtt aagcctttta aactcatggt agttattttc aagcattaac atgaacttaa + 1801 attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt agttctttta + 1861 ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca tgttccagat + 1921 tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca ctaaaaacta + 1981 attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc tcaaagcctt + 2041 taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt gctttagcta + 2101 atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg ttataagtga + 2161 acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt agtgccacac + 2221 agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc gctagttcat + 2281 ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga ttttaatcac + 2341 tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt tgagttgtgg + 2401 gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct agaccctctg + 2461 taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt ataatttata + 2521 gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg tataactcac + 2581 tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt gctcctctac + 2641 aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg gttgcggccg + 2701 caatcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg + 2761 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg + 2821 gcactacagg cgcggcgcgc ccagctgtct agggcggcgg atttgtccta ctcaggagag + 2881 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt + 2941 ttatttgatg cctttaatta aggctcggga gacctatcgg taataacagt ccaatctggt + 3001 gtaacttcgg aatcgtcccc aattattgaa cacccttcgg ggtgtttttt tgtttctggt + 3061 ctaccatctc gttgtgataa tagacctgaa gtgcctactc tggaaaatct ttgacagcta + 3121 gctcagtcct aggtataatg ctagcagctg tcaccggatg tgctttccgg tctgatgagt + 3181 ccgtgaggac gaaacagcct ctacaaataa ttttgtttaa ggctcgctac gagaagattg + 3241 ttactttcgc agcgttatta tctaaggagg tagtccatgg ttagcaaagg cgaggcggtt + 3301 atcaaggagt ttatgcgttt taaggttcac atggagggta gcatgaatgg tcacgagttc + 3361 gagatcgagg gtgaaggcga gggtcgtccg tacgaaggca cccagaccgc gaagctgaaa + 3421 gtgaccaagg gtggcccgct gccgttcagc tgggacatcc tgagcccgca gttcatgtat + 3481 ggcagccgtg cgtttaccaa acacccggcg gacattccgg attactataa gcaaagcttc + 3541 ccggaaggtt ttaaatggga gcgtgttatg aacttcgaag atggtggcgc ggtgaccgtt + 3601 acccaggaca ccagcctgga ggatggcacc ctgatttaca aggtgaaact gcgtggcacc + 3661 aactttccgc cggatggtcc ggttatgcag aagaaaacga tgggttggga agcgagcacc + 3721 gagcgtctgt atccggaaga tggcgtgctg aagggtgata tcaaaatggc gctgcgtctg + 3781 aaggacggtg gccgttacct ggcggatttt aagaccacct ataaagcgaa gaaaccggtg + 3841 caaatgccgg gtgcgtacaa cgttgaccgt aaactggata ttaccagcca caacgaggat + 3901 tataccgtgg ttgagcaata tgagcgtagc gagggtcgcc acagcaccgg cggcatggac + 3961 gaactgtata agggatccta ataa // -LOCUS BASIC_SEVA_25.10 5821 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_25a.10 5821 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Kanamycin resistance marker and RSF1010 - origin of replication.. -ACCESSION BASIC_SEVA_25 -VERSION BASIC_SEVA_25.10 + origin of replication. +ACCESSION 4683b5e8bb9714875a28a0880c88f904 +VERSION 4683b5e8bb9714875a28a0880c88f904 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -607,8 +521,8 @@ FEATURES Location/Qualifiers /label="SEVA_Km" misc_feature 1132..1184 /label="BSEVA_L1" - misc_feature 1193..4866 - /label="SEVA_RSF101" + misc_feature 1196..4866 + /label="SEVA_RSF1010(x5ax)" misc_feature 4881..4985 /label="SEVA_T1" misc_feature 4997..5051 @@ -639,61 +553,61 @@ ORIGIN 961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc 1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat 1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcg gctcggccca - 1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg cctcagcctg - 1201 ccgccttggg ccgggtgatg tcgtacttgc ccgccgcgaa ctcggttacc gtccagccca - 1261 gcgcgaccag ctccggcaac gcctcgcgca cccgctggcg gcgcttgcgc atggtcgaac - 1321 cactggcctc tgacggccag acatagccgc acaaggtatc tatggaagcc ttgccggttt - 1381 tgccggggtc gatccagcca cacagccgct ggtgcagcag gcgggcggtt tcgctgtcca - 1441 gcgcccgcac ctcgtccatg ctgatgcgca catgctggcc gccacccatg acggcctgcg - 1501 cgatcaaggg gttcagggcc acgtacaggc gcccgtccgc ctcgtcgctg gcgtactccg - 1561 acagcagccg aaacccctgc cgcttgcggc cattctgggc gatgatggat accttccaaa - 1621 ggcgctcgat gcagtcctgt atgtgcttga gcgccccacc actatcgacc tctgccccga - 1681 tttcctttgc cagcgcccga tagctacctt tgaccacatg gcattcagcg gtgacggcct - 1741 cccacttggg ttccaggaac agccggagct gccgtccgcc ttcggtcttg ggttccgggc - 1801 caagcactag gccattaggc ccagccatgg ccaccagccc ttgcaggatg cgcagatcat - 1861 cagcgcccag cggctccggg ccgctgaact cgatccgctt gccgtcgccg tagtcatacg - 1921 tcacgtccag cttgctgcgc ttgcgctcgc cccgcttgag ggcacggaac aggccggggg - 1981 ccagacagtg cgccgggtcg tgccggacgt ggctgaggct gtgcttgttc ttaggcttca - 2041 ccacggggca cccccttgct cttgcgctgc ctctccagca cggcgggctt gagcaccccg - 2101 ccgtcatgcc gcctgaacca ccgatcagcg aacggtgcgc catagttggc cttgctcaca - 2161 ccgaagcgga cgaagaaccg gcgctggtcg tcgtccacac cccattcctc ggcctcggcg - 2221 ctggtcatgc tcgacaggta ggactgccag cggatgttat cgaccagtac cgagctgccc - 2281 cggctggcct gctgctggtc gcctgcgccc atcatggccg cgcccttgct ggcatggtgc - 2341 aggaacacga tagagcaccc ggtatcggcg gcgatggcct ccatgcgacc gatgacctgg - 2401 gccatggggc cgctggcgtt ttcttcctcg atgtggaacc ggcgcagcgt gtccagcacc - 2461 atcaggcggc ggccctcggc ggcgcgcttg aggccgtcga accactccgg ggccatgatg - 2521 ttgggcaggc tgccgatcag cggctggatc agcaggccgt cagccacggc ttgccgttcc - 2581 tcggcgctga ggtgcgcccc aagggcgtgc aggcggtgat gaatggcggt gggcgggtct - 2641 tcggcgggca ggtagatcac cgggccggtg ggcagttcgc ccacctccag cagatccggc - 2701 ccgcctgcaa tctgtgcggc cagttgcagg gccagcatgg atttaccggc accaccgggc - 2761 gacaccagcg ccccgaccgt accggccacc atgttgggca aaacgtagtc cagcggtggc - 2821 ggcgctgctg cgaacgcctc cagaatattg ataggcttat gggtagccat tgattgcctc - 2881 ctttgcaggc agttggtggt taggcgctgg cggggtcact acccccgccc tgcgccgctc - 2941 tgagttcttc caggcactcg cgcagcgcct cgtattcgtc gtcggtcagc cagaacttgc - 3001 gctgacgcat ccctttggcc ttcatgcgct cggcatatcg cgcttggcgt acagcgtcag - 3061 ggctggccag caggtcgccg gtctgcttgt ccttttggtc tttcatatca gtcaccgaga - 3121 aacttgccgg ggccgaaagg cttgtcttcg cggaacaagg acaaggtgca gccgtcaagg - 3181 ttaaggctgg ccatatcagc gactgaaaag cggccagcct cggccttgtt tgacgtataa - 3241 ccaaagccac cgggcaacca atagcccttg tcacttttga tcaggtagac cgaccctgaa - 3301 gcgctttttt cgtattccat aaaaccccct tctgtgcgtg agtactcata gtataacagg - 3361 cgtgagtacc aacgcaagca ctacatgctg aaatctggcc cgcccctgtc catgcctcgc - 3421 tggcggggtg ccggtgcccg tgccagctcg gcccgcgcaa gctggacgct gggcagaccc - 3481 atgaccttgc tgacggtgcg ctcgatgtaa tccgcttcgt ggccgggctt gcgctctgcc - 3541 agcgctgggc tggcctcggc catggccttg ccgatttcct cggcactgcg gccccggctg - 3601 gccagcttct gcgcggcgat aaagtcgcac ttgctgaggt catcaccgaa gcgcttgacc - 3661 agcccggcca tctcgctgcg gtactcgtcc agcgccgtgc gccggtggcg gctaagctgc - 3721 cgctcgggca gttcgaggct ggccagcctg cgggccttct cctgctgccg ctgggcctgc - 3781 tcgatctgct ggccagcctg ctgcaccagc gccgggccag cggtggcggt cttgcccttg - 3841 gattcacgca gcagcaccca cggctgataa ccggcgcggg tggtgtgctt gtccttgcgg - 3901 ttggtgaagc ccgccaagcg gccatagtgg cggctgtcgg cgctggccgg gtcggcgtcg - 3961 tactcgctgg ccagcgtccg ggcaatctgc ccccgaagtt caccgcctgc ggcgtcggcc - 4021 accttgaccc atgcctgata gttcttcggg ctggtttcca ctaccagggc aggctcccgg - 4081 ccctcggctt tcatgtcatc caggtcaaac tcgctgaggt cgtccaccag caccagacca - 4141 tgccgctcct gctcggcggg cctgatatac acgtcattgc cctgggcatt catccgcttg - 4201 agccatggcg tgttctggag cacttcggcg gctgaccatt cccggttcat catctggccg - 4261 gtggtggcgt ccctgacgcc gatatcgaag cgctcacagc ccatggcctt gagctgtcgg - 4321 cctatggcct gcaaagtcct gtcgttcttc atcgggccac caagcgattc ccacacatta - 4381 tacgagccgg aagcataaag tgtaaagcct agatccgaag gatgagccgg gctgaatgat + 1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccgtaggc cggcctcagc + 1201 ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt accgtccagc + 1261 ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg cgcatggtcg + 1321 aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa gccttgccgg + 1381 ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg gtttcgctgt + 1441 ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc atgacggcct + 1501 gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg ctggcgtact + 1561 ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg gataccttcc + 1621 aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg acctctgccc + 1681 cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca gcggtgacgg + 1741 cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc ttgggttccg + 1801 ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg atgcgcagat + 1861 catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg ccgtagtcat + 1921 acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg aacaggccgg + 1981 gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg ttcttaggct + 2041 tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg cttgagcacc + 2101 ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt ggccttgctc + 2161 acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc ctcggcctcg + 2221 gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag taccgagctg + 2281 ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt gctggcatgg + 2341 tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg accgatgacc + 2401 tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag cgtgtccagc + 2461 accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc cggggccatg + 2521 atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac ggcttgccgt + 2581 tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc ggtgggcggg + 2641 tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc cagcagatcc + 2701 ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc ggcaccaccg + 2761 ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta gtccagcggt + 2821 ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc cattgattgc + 2881 ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg ccctgcgccg + 2941 ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc agccagaact + 3001 tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg cgtacagcgt + 3061 cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata tcagtcaccg + 3121 agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt gcagccgtca + 3181 aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt gtttgacgta + 3241 taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta gaccgaccct + 3301 gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc atagtataac + 3361 aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct gtccatgcct + 3421 cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac gctgggcaga + 3481 cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg cttgcgctct + 3541 gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact gcggccccgg + 3601 ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc gaagcgcttg + 3661 accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg gcggctaagc + 3721 tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg ccgctgggcc + 3781 tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc ggtcttgccc + 3841 ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg cttgtccttg + 3901 cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc cgggtcggcg + 3961 tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc tgcggcgtcg + 4021 gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag ggcaggctcc + 4081 cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac cagcaccaga + 4141 ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc attcatccgc + 4201 ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt catcatctgg + 4261 ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc cttgagctgt + 4321 cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga ttcccacaca + 4381 ttatacgagc cggaagcata aagtgtagct agatccgaag gatgagccgg gctgaatgat 4441 cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt agggggaaag 4501 gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg tggggtttag 4561 cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta gcctagcgca @@ -719,11 +633,11 @@ ORIGIN 5761 gcgtagcgag ggtcgccaca gcaccggcgg catggacgaa ctgtataagg gatcctaata 5821 a // -LOCUS BASIC_SEVA_26.10 2879 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_26.10 2879 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Kanamycin resistance marker and p15A - origin of replication.. -ACCESSION BASIC_SEVA_26 -VERSION BASIC_SEVA_26.10 + origin of replication. +ACCESSION b484d417d0b514190db028347fa66fba +VERSION b484d417d0b514190db028347fa66fba KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -799,11 +713,11 @@ ORIGIN 2761 accgtaaact ggatattacc agccacaacg aggattatac cgtggttgag caatatgagc 2821 gtagcgaggg tcgccacagc accggcggca tggacgaact gtataaggga tcctaataa // -LOCUS BASIC_SEVA_27.10 3803 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_27.10 3803 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Kanamycin resistance marker and pSC101 - origin of replication.. -ACCESSION BASIC_SEVA_27 -VERSION BASIC_SEVA_27.10 + origin of replication. +ACCESSION 3b433d3fd1e298bff6d5d066a372d067 +VERSION 3b433d3fd1e298bff6d5d066a372d067 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -895,11 +809,11 @@ ORIGIN 3721 ataccgtggt tgagcaatat gagcgtagcg agggtcgcca cagcaccggc ggcatggacg 3781 aactgtataa gggatcctaa taa // -LOCUS BASIC_SEVA_27_TS.10 3872 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_29.10 3532 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Kanamycin resistance marker and - temperature sensitive pSC101 origin of replication.. -ACCESSION BASIC_SEVA_27_TS -VERSION BASIC_SEVA_27_TS.10 + pBR322-ROP origin of replication. +ACCESSION b0dbd8bc2d60e1a7aad51780fcdbffd8 +VERSION b0dbd8bc2d60e1a7aad51780fcdbffd8 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -913,116 +827,17 @@ FEATURES Location/Qualifiers /label="SEVA_Km" misc_feature 1132..1184 /label="BSEVA_L1" - CDS complement(1246..2196) - /label="Rep101(Ts)" - rep_origin 2244..2466 - /label="pSC101 ori" - misc_feature 2736..2840 + misc_feature 1193..2577 + /label="SEVA_pBR322-ROP" + misc_feature 2592..2696 /label="SEVA_T1" - misc_feature 2852..2906 + misc_feature 2708..2762 /label="LMS" - misc_feature 2999..3033 - /label="J23119" - misc_feature 3151..3158 - /label="RBS-A12" - CDS 3165..3869 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttaa atttgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat - 241 catcatgaac aataaaactg tctgcttaca taaacagtaa tacaaggggt gttatgagcc - 301 atattcagcg tgaaacgagc tgtagccgtc cgcgtctgaa cagcaacatg gatgcggatc - 361 tgtatggcta taaatgggcg cgtgataacg tgggtcagag cggcgcgacc atttatcgtc - 421 tgtatggcaa accggatgcg ccggaactgt ttctgaaaca tggcaaaggc agcgtggcga - 481 acgatgtgac cgatgaaatg gtgcgtctga actggctgac cgaatttatg ccgctgccga - 541 ccattaaaca ttttattcgc accccggatg atgcgtggct gctgaccacc gcgattccgg - 601 gcaaaaccgc gtttcaggtg ctggaagaat atccggatag cggcgaaaac attgtggatg - 661 cgctggccgt gtttctgcgt cgtctgcata gcattccggt gtgcaactgc ccgtttaaca - 721 gcgatcgtgt gtttcgtctg gcccaggcgc agagccgtat gaacaacggc ctggtggatg - 781 cgagcgattt tgatgatgaa cgtaacggct ggccggtgga acaggtgtgg aaagaaatgc - 841 ataaactgct gccgtttagc ccggatagcg tggtgaccca cggcgatttt agcctggata - 901 acctgatttt cgatgaaggc aaactgattg gctgcattga tgtgggccgt gtgggcattg - 961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc - 1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat - 1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcg gctcggccca - 1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtcctgttct acttttgttt - 1201 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta - 1261 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc - 1321 attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg - 1381 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta ggtaggaatc - 1441 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc - 1501 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg - 1561 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg - 1621 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat - 1681 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag - 1741 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg - 1801 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact - 1861 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc - 1921 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc - 1981 tttagctaat acaccataag cattttccct actgatgttc atcatctgaa cgtattggtt - 2041 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag - 2101 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc - 2161 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt - 2221 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg - 2281 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag - 2341 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat - 2401 aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta - 2461 taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc - 2521 tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggt - 2581 tgcggccgca atcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg - 2641 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg - 2701 ggtaaatggc actacaggcg cggcgcgccc agctgtctag ggcggcggat ttgtcctact - 2761 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc - 2821 ctttcgtttt atttgatgcc tttaattaag gctcgggaga cctatcggta ataacagtcc - 2881 aatctggtgt aacttcggaa tcgtccccaa ttattgaaca cccttcgggg tgtttttttg - 2941 tttctggtct accatctcgt tgtgataata gacctgaagt gcctactctg gaaaatcttt - 3001 gacagctagc tcagtcctag gtataatgct agcagctgtc accggatgtg ctttccggtc - 3061 tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaagg ctcgctacga - 3121 gaagattgtt actttcgcag cgttattatc taaggaggta gtccatggtt agcaaaggcg - 3181 aggcggttat caaggagttt atgcgtttta aggttcacat ggagggtagc atgaatggtc - 3241 acgagttcga gatcgagggt gaaggcgagg gtcgtccgta cgaaggcacc cagaccgcga - 3301 agctgaaagt gaccaagggt ggcccgctgc cgttcagctg ggacatcctg agcccgcagt - 3361 tcatgtatgg cagccgtgcg tttaccaaac acccggcgga cattccggat tactataagc - 3421 aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa cttcgaagat ggtggcgcgg - 3481 tgaccgttac ccaggacacc agcctggagg atggcaccct gatttacaag gtgaaactgc - 3541 gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa gaaaacgatg ggttgggaag - 3601 cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa gggtgatatc aaaatggcgc - 3661 tgcgtctgaa ggacggtggc cgttacctgg cggattttaa gaccacctat aaagcgaaga - 3721 aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa actggatatt accagccaca - 3781 acgaggatta taccgtggtt gagcaatatg agcgtagcga gggtcgccac agcaccggcg - 3841 gcatggacga actgtataag ggatcctaat aa -// -LOCUS BASIC_SEVA_28.10 3076 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Kanamycin resistance marker and pUC - origin of replication.. -ACCESSION BASIC_SEVA_28 -VERSION BASIC_SEVA_28.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 193..1119 - /label="SEVA_Km" - misc_feature 1132..1184 - /label="BSEVA_L1" - misc_feature 1193..2121 - /label="SEVA_pUC" - misc_feature 2136..2240 - /label="SEVA_T1" - misc_feature 2252..2306 - /label="LMS" - misc_feature 2307..2341 - /label="J23106" - misc_feature 2350..2361 - /label="B0034" - CDS 2369..3073 + misc_feature 2763..2797 + /label="J23106" + misc_feature 2806..2817 + /label="B0034" + CDS 2825..3529 /label="mScarlet" ORIGIN 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact @@ -1047,42 +862,49 @@ ORIGIN 1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg ccccgtagaa 1201 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 1261 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt - 1321 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg + 1321 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 1381 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 1441 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 1501 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc - 1561 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct ttgagaaagc + 1561 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 1621 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 1681 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 1741 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 1801 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct 1861 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag 1921 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa - 1981 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc - 2041 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg - 2101 agttagctca ctcattaggc aggcgcgccc agctgtctag ggcggcggat ttgtcctact - 2161 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc - 2221 ctttcgtttt atttgatgcc tttaattaag gctcgggaga cctatcggta ataacagtcc - 2281 aatctggtgt aacttcggaa tcgtccttta cggctagctc agtcctaggt atagtgctag - 2341 ctactagtga aagaggagaa atactagtat ggttagcaaa ggcgaggcgg ttatcaagga - 2401 gtttatgcgt tttaaggttc acatggaggg tagcatgaat ggtcacgagt tcgagatcga - 2461 gggtgaaggc gagggtcgtc cgtacgaagg cacccagacc gcgaagctga aagtgaccaa - 2521 gggtggcccg ctgccgttca gctgggacat cctgagcccg cagttcatgt atggcagccg - 2581 tgcgtttacc aaacacccgg cggacattcc ggattactat aagcaaagct tcccggaagg - 2641 ttttaaatgg gagcgtgtta tgaacttcga agatggtggc gcggtgaccg ttacccagga - 2701 caccagcctg gaggatggca ccctgattta caaggtgaaa ctgcgtggca ccaactttcc - 2761 gccggatggt ccggttatgc agaagaaaac gatgggttgg gaagcgagca ccgagcgtct - 2821 gtatccggaa gatggcgtgc tgaagggtga tatcaaaatg gcgctgcgtc tgaaggacgg - 2881 tggccgttac ctggcggatt ttaagaccac ctataaagcg aagaaaccgg tgcaaatgcc - 2941 gggtgcgtac aacgttgacc gtaaactgga tattaccagc cacaacgagg attataccgt - 3001 ggttgagcaa tatgagcgta gcgagggtcg ccacagcacc ggcggcatgg acgaactgta - 3061 taagggatcc taataa + 1981 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc + 2041 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc + 2101 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg + 2161 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg + 2221 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt + 2281 aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca + 2341 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa + 2401 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg + 2461 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac + 2521 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggggc + 2581 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca + 2641 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgccttta + 2701 attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact tcggaatcgt + 2761 cctttacggc tagctcagtc ctaggtatag tgctagctac tagtgaaaga ggagaaatac + 2821 tagtatggtt agcaaaggcg aggcggttat caaggagttt atgcgtttta aggttcacat + 2881 ggagggtagc atgaatggtc acgagttcga gatcgagggt gaaggcgagg gtcgtccgta + 2941 cgaaggcacc cagaccgcga agctgaaagt gaccaagggt ggcccgctgc cgttcagctg + 3001 ggacatcctg agcccgcagt tcatgtatgg cagccgtgcg tttaccaaac acccggcgga + 3061 cattccggat tactataagc aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa + 3121 cttcgaagat ggtggcgcgg tgaccgttac ccaggacacc agcctggagg atggcaccct + 3181 gatttacaag gtgaaactgc gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa + 3241 gaaaacgatg ggttgggaag cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa + 3301 gggtgatatc aaaatggcgc tgcgtctgaa ggacggtggc cgttacctgg cggattttaa + 3361 gaccacctat aaagcgaaga aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa + 3421 actggatatt accagccaca acgaggatta taccgtggtt gagcaatatg agcgtagcga + 3481 gggtcgccac agcaccggcg gcatggacga actgtataag ggatcctaat aa // -LOCUS BASIC_SEVA_29.10 3532 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_27_pKD46.10 3872 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Kanamycin resistance marker and - pBR322-ROP origin of replication.. -ACCESSION BASIC_SEVA_29 -VERSION BASIC_SEVA_29.10 + temperature sensitive pSC101 origin of replication. +ACCESSION 016b73059a8fa9268d8b35d2bdfa96ed +VERSION 016b73059a8fa9268d8b35d2bdfa96ed KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -1096,17 +918,19 @@ FEATURES Location/Qualifiers /label="SEVA_Km" misc_feature 1132..1184 /label="BSEVA_L1" - misc_feature 1193..2577 - /label="SEVA_pBR322-ROP" - misc_feature 2592..2696 + CDS 1246..2196 + /label="Rep101(pKD46)" + rep_origin 2244..2466 + /label="pSC101 ori" + misc_feature 2736..2840 /label="SEVA_T1" - misc_feature 2708..2762 + misc_feature 2852..2906 /label="LMS" - misc_feature 2763..2797 + misc_feature 2999..3033 /label="J23119" - misc_feature 2806..2817 - /label="B0034" - CDS 2825..3529 + misc_feature 3151..3158 + /label="RBS-A12" + CDS 3165..3869 /label="mScarlet" ORIGIN 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact @@ -1128,52 +952,58 @@ ORIGIN 961 cggatcgtta tcaggatctg gccattctgt ggaactgcct gggcgaattt agcccgagcc 1021 tgcaaaaacg tctgtttcag aaatatggca ttgataatcc ggatatgaac aaactgcaat 1081 ttcatctgat gctggatgaa tttttctaat aattaattgg accgcggtcg gctcggccca - 1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg ccccgtagaa - 1201 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca - 1261 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt - 1321 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg - 1381 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc - 1441 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga - 1501 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc - 1561 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc - 1621 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca - 1681 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg - 1741 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta - 1801 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct - 1861 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag - 1921 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa - 1981 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc - 2041 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc - 2101 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg - 2161 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg - 2221 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt - 2281 aaagctcatc agcgtggtcg tgcagcgatt cacagatgtc tgcctgttca tccgcgtcca - 2341 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa - 2401 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg - 2461 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac - 2521 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggggc - 2581 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca - 2641 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgccttta - 2701 attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact tcggaatcgt - 2761 ccttgacagc tagctcagtc ctaggtataa tgctagctac tagtgaaaga ggagaaatac - 2821 tagtatggtt agcaaaggcg aggcggttat caaggagttt atgcgtttta aggttcacat - 2881 ggagggtagc atgaatggtc acgagttcga gatcgagggt gaaggcgagg gtcgtccgta - 2941 cgaaggcacc cagaccgcga agctgaaagt gaccaagggt ggcccgctgc cgttcagctg - 3001 ggacatcctg agcccgcagt tcatgtatgg cagccgtgcg tttaccaaac acccggcgga - 3061 cattccggat tactataagc aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa - 3121 cttcgaagat ggtggcgcgg tgaccgttac ccaggacacc agcctggagg atggcaccct - 3181 gatttacaag gtgaaactgc gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa - 3241 gaaaacgatg ggttgggaag cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa - 3301 gggtgatatc aaaatggcgc tgcgtctgaa ggacggtggc cgttacctgg cggattttaa - 3361 gaccacctat aaagcgaaga aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa - 3421 actggatatt accagccaca acgaggatta taccgtggtt gagcaatatg agcgtagcga - 3481 gggtcgccac agcaccggcg gcatggacga actgtataag ggatcctaat aa + 1141 cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtcctgttct acttttgttt + 1201 gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga tccttccgta + 1261 tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg agaacgaacc + 1321 attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac tggtgagctg + 1381 aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta ggtaggaatc + 1441 tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt tctcaagttc + 1501 ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat cagtcgggcg + 1561 gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt tacttattgg + 1621 tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa gcattaacat + 1681 gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct tttgtgttag + 1741 ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag acttaacatg + 1801 ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata tctcttcact + 1861 aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact ggaaaatctc + 1921 aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct ctctggttgc + 1981 tttagctaat acaccataag cattttccct actgatgttc atcatctgag cgtattggtt + 2041 ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg ggttgagtag + 2101 tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc gactaatcgc + 2161 tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt ctaggtgatt + 2221 ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc ttttcctttg + 2281 agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa attctgctag + 2341 accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc aagtggttat + 2401 aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca gccctgtgta + 2461 taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa cgctgtttgc + 2521 tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg caagctcggt + 2581 tgcggccgca atcgggcaaa tcgctgaata ttccttttgt ctccgaccat caggcacctg + 2641 agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc agtgaatggg + 2701 ggtaaatggc actacaggcg cggcgcgccc agctgtctag ggcggcggat ttgtcctact + 2761 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc + 2821 ctttcgtttt atttgatgcc tttaattaag gctcgggaga cctatcggta ataacagtcc + 2881 aatctggtgt aacttcggaa tcgtccccaa ttattgaaca cccttcgggg tgtttttttg + 2941 tttctggtct accatctcgt tgtgataata gacctgaagt gcctactctg gaaaatcttt + 3001 gacagctagc tcagtcctag gtataatgct agcagctgtc accggatgtg ctttccggtc + 3061 tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaagg ctcgctacga + 3121 gaagattgtt actttcgcag cgttattatc taaggaggta gtccatggtt agcaaaggcg + 3181 aggcggttat caaggagttt atgcgtttta aggttcacat ggagggtagc atgaatggtc + 3241 acgagttcga gatcgagggt gaaggcgagg gtcgtccgta cgaaggcacc cagaccgcga + 3301 agctgaaagt gaccaagggt ggcccgctgc cgttcagctg ggacatcctg agcccgcagt + 3361 tcatgtatgg cagccgtgcg tttaccaaac acccggcgga cattccggat tactataagc + 3421 aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa cttcgaagat ggtggcgcgg + 3481 tgaccgttac ccaggacacc agcctggagg atggcaccct gatttacaag gtgaaactgc + 3541 gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa gaaaacgatg ggttgggaag + 3601 cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa gggtgatatc aaaatggcgc + 3661 tgcgtctgaa ggacggtggc cgttacctgg cggattttaa gaccacctat aaagcgaaga + 3721 aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa actggatatt accagccaca + 3781 acgaggatta taccgtggtt gagcaatatg agcgtagcga gggtcgccac agcaccggcg + 3841 gcatggacga actgtataag ggatcctaat aa // -LOCUS BASIC_SEVA_35.10 5677 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_35a.10 5677 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Chloramphenicol resistance marker and - RSF1010 origin of replication.. -ACCESSION BASIC_SEVA_35 -VERSION BASIC_SEVA_35.10 + RSF1010 origin of replication. +ACCESSION 01bb230a5ebe20dc3196b1f760d42d94 +VERSION 01bb230a5ebe20dc3196b1f760d42d94 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -1187,8 +1017,8 @@ FEATURES Location/Qualifiers /label="SEVA_Cm" misc_feature 988..1040 /label="BSEVA_L1" - misc_feature 1049..4722 - /label="SEVA_RSF101" + misc_feature 1052..4722 + /label="SEVA_RSF1010(x5ax)" misc_feature 4737..4841 /label="SEVA_T1" misc_feature 4853..4907 @@ -1217,61 +1047,61 @@ ORIGIN 841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt 901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag 961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga - 1021 ggtccacaca gatagtgtcc ggccggcctc agcctgccgc cttgggccgg gtgatgtcgt - 1081 acttgcccgc cgcgaactcg gttaccgtcc agcccagcgc gaccagctcc ggcaacgcct - 1141 cgcgcacccg ctggcggcgc ttgcgcatgg tcgaaccact ggcctctgac ggccagacat - 1201 agccgcacaa ggtatctatg gaagccttgc cggttttgcc ggggtcgatc cagccacaca - 1261 gccgctggtg cagcaggcgg gcggtttcgc tgtccagcgc ccgcacctcg tccatgctga - 1321 tgcgcacatg ctggccgcca cccatgacgg cctgcgcgat caaggggttc agggccacgt - 1381 acaggcgccc gtccgcctcg tcgctggcgt actccgacag cagccgaaac ccctgccgct - 1441 tgcggccatt ctgggcgatg atggatacct tccaaaggcg ctcgatgcag tcctgtatgt - 1501 gcttgagcgc cccaccacta tcgacctctg ccccgatttc ctttgccagc gcccgatagc - 1561 tacctttgac cacatggcat tcagcggtga cggcctccca cttgggttcc aggaacagcc - 1621 ggagctgccg tccgccttcg gtcttgggtt ccgggccaag cactaggcca ttaggcccag - 1681 ccatggccac cagcccttgc aggatgcgca gatcatcagc gcccagcggc tccgggccgc - 1741 tgaactcgat ccgcttgccg tcgccgtagt catacgtcac gtccagcttg ctgcgcttgc - 1801 gctcgccccg cttgagggca cggaacaggc cgggggccag acagtgcgcc gggtcgtgcc - 1861 ggacgtggct gaggctgtgc ttgttcttag gcttcaccac ggggcacccc cttgctcttg - 1921 cgctgcctct ccagcacggc gggcttgagc accccgccgt catgccgcct gaaccaccga - 1981 tcagcgaacg gtgcgccata gttggccttg ctcacaccga agcggacgaa gaaccggcgc - 2041 tggtcgtcgt ccacacccca ttcctcggcc tcggcgctgg tcatgctcga caggtaggac - 2101 tgccagcgga tgttatcgac cagtaccgag ctgccccggc tggcctgctg ctggtcgcct - 2161 gcgcccatca tggccgcgcc cttgctggca tggtgcagga acacgataga gcacccggta - 2221 tcggcggcga tggcctccat gcgaccgatg acctgggcca tggggccgct ggcgttttct - 2281 tcctcgatgt ggaaccggcg cagcgtgtcc agcaccatca ggcggcggcc ctcggcggcg - 2341 cgcttgaggc cgtcgaacca ctccggggcc atgatgttgg gcaggctgcc gatcagcggc - 2401 tggatcagca ggccgtcagc cacggcttgc cgttcctcgg cgctgaggtg cgccccaagg - 2461 gcgtgcaggc ggtgatgaat ggcggtgggc gggtcttcgg cgggcaggta gatcaccggg - 2521 ccggtgggca gttcgcccac ctccagcaga tccggcccgc ctgcaatctg tgcggccagt - 2581 tgcagggcca gcatggattt accggcacca ccgggcgaca ccagcgcccc gaccgtaccg - 2641 gccaccatgt tgggcaaaac gtagtccagc ggtggcggcg ctgctgcgaa cgcctccaga - 2701 atattgatag gcttatgggt agccattgat tgcctccttt gcaggcagtt ggtggttagg - 2761 cgctggcggg gtcactaccc ccgccctgcg ccgctctgag ttcttccagg cactcgcgca - 2821 gcgcctcgta ttcgtcgtcg gtcagccaga acttgcgctg acgcatccct ttggccttca - 2881 tgcgctcggc atatcgcgct tggcgtacag cgtcagggct ggccagcagg tcgccggtct - 2941 gcttgtcctt ttggtctttc atatcagtca ccgagaaact tgccggggcc gaaaggcttg - 3001 tcttcgcgga acaaggacaa ggtgcagccg tcaaggttaa ggctggccat atcagcgact - 3061 gaaaagcggc cagcctcggc cttgtttgac gtataaccaa agccaccggg caaccaatag - 3121 cccttgtcac ttttgatcag gtagaccgac cctgaagcgc ttttttcgta ttccataaaa - 3181 cccccttctg tgcgtgagta ctcatagtat aacaggcgtg agtaccaacg caagcactac - 3241 atgctgaaat ctggcccgcc cctgtccatg cctcgctggc ggggtgccgg tgcccgtgcc - 3301 agctcggccc gcgcaagctg gacgctgggc agacccatga ccttgctgac ggtgcgctcg - 3361 atgtaatccg cttcgtggcc gggcttgcgc tctgccagcg ctgggctggc ctcggccatg - 3421 gccttgccga tttcctcggc actgcggccc cggctggcca gcttctgcgc ggcgataaag - 3481 tcgcacttgc tgaggtcatc accgaagcgc ttgaccagcc cggccatctc gctgcggtac - 3541 tcgtccagcg ccgtgcgccg gtggcggcta agctgccgct cgggcagttc gaggctggcc - 3601 agcctgcggg ccttctcctg ctgccgctgg gcctgctcga tctgctggcc agcctgctgc - 3661 accagcgccg ggccagcggt ggcggtcttg cccttggatt cacgcagcag cacccacggc - 3721 tgataaccgg cgcgggtggt gtgcttgtcc ttgcggttgg tgaagcccgc caagcggcca - 3781 tagtggcggc tgtcggcgct ggccgggtcg gcgtcgtact cgctggccag cgtccgggca - 3841 atctgccccc gaagttcacc gcctgcggcg tcggccacct tgacccatgc ctgatagttc - 3901 ttcgggctgg tttccactac cagggcaggc tcccggccct cggctttcat gtcatccagg - 3961 tcaaactcgc tgaggtcgtc caccagcacc agaccatgcc gctcctgctc ggcgggcctg - 4021 atatacacgt cattgccctg ggcattcatc cgcttgagcc atggcgtgtt ctggagcact - 4081 tcggcggctg accattcccg gttcatcatc tggccggtgg tggcgtccct gacgccgata - 4141 tcgaagcgct cacagcccat ggccttgagc tgtcggccta tggcctgcaa agtcctgtcg - 4201 ttcttcatcg ggccaccaag cgattcccac acattatacg agccggaagc ataaagtgta - 4261 aagcctagat ccgaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc + 1021 ggtccacaca gatagtgtcc gtaggccggc ctcagcctgc cgccttgggc cgggtgatgt + 1081 cgtacttgcc cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg + 1141 cctcgcgcac ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga + 1201 catagccgca caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac + 1261 acagccgctg gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc + 1321 tgatgcgcac atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca + 1381 cgtacaggcg cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc + 1441 gcttgcggcc attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta + 1501 tgtgcttgag cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat + 1561 agctaccttt gaccaccatg gcatcagcgg tgacggcctc ccacttgggt tccaggaaca + 1621 gccggagctg ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc + 1681 cagccatggc caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc + 1741 cgctgaactc gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct + 1801 tgcgctcgcc ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt + 1861 gccggacgtg gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc + 1921 ttgcgctgcc tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac + 1981 cgatcagcga acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg + 2041 cgctggtcgt cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag + 2101 gactgccagc ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg + 2161 cctgcgccca tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg + 2221 gtatcggcgg cgatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt + 2281 tcttcctcga tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg + 2341 gcgcgcttga ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc + 2401 ggctggatca gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca + 2461 agggcgtgca ggcggtgatg aatggcggtg ggcgggtctt cggcgggcag gtagatcacc + 2521 gggccggtgg gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc + 2581 agttgcaggg ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta + 2641 ccggccacca tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc + 2701 agaatattga taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt + 2761 aggcgctggc ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc + 2821 gcagcgcctc gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct + 2881 tcatgcgctc ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg + 2941 tctgcttgtc cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc + 3001 ttgtcttcgc ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg + 3061 actgaaaagc ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa + 3121 tagcccttgt cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata + 3181 aaaccccctt ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac + 3241 tacatgctga aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt + 3301 gccagctcgg cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc + 3361 tcgatgtaat ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc + 3421 atggccttgc cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata + 3481 aagtcgcact tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg + 3541 tactcgtcca gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg + 3601 gccagcctgc gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc + 3661 tgcaccagcg ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac + 3721 ggctgataac cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg + 3781 ccatagtggc ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg + 3841 gcaatctgcc cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag + 3901 ttcttcgggc tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc + 3961 aggtcaaact cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc + 4021 ctgatataca cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc + 4081 acttcggcgg ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg + 4141 atatcgaagc gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg + 4201 tcgttcttca tcgggccacc aagcgattcc cacacattat acgagccgga agcataaagt + 4261 gtagctagat ccgaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc 4321 tgcacacgcg cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct 4381 ccagcgtatt tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct 4441 gccgcgcagc ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct @@ -1296,11 +1126,11 @@ ORIGIN 5581 ccacaacgag gattataccg tggttgagca atatgagcgt agcgagggtc gccacagcac 5641 cggcggcatg gacgaactgt ataagggatc ctaataa // -LOCUS BASIC_SEVA_36.10 2735 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_36.10 2735 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Chloramphenicol resistance marker and - p15A origin of replication.. -ACCESSION BASIC_SEVA_36 -VERSION BASIC_SEVA_36.10 + p15A origin of replication. +ACCESSION 1413513c978a56f243171c4f4cf6aaac +VERSION 1413513c978a56f243171c4f4cf6aaac KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -1374,11 +1204,11 @@ ORIGIN 2641 acaacgagga ttataccgtg gttgagcaat atgagcgtag cgagggtcgc cacagcaccg 2701 gcggcatgga cgaactgtat aagggatcct aataa // -LOCUS BASIC_SEVA_37.10 3659 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_37.10 3659 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Chloramphenicol resistance marker and - pSC101 origin of replication.. -ACCESSION BASIC_SEVA_37 -VERSION BASIC_SEVA_37.10 + pSC101 origin of replication. +ACCESSION eceac71fa048bd052921bf134322c381 +VERSION eceac71fa048bd052921bf134322c381 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -1467,189 +1297,11 @@ ORIGIN 3541 accgtaaact ggatattacc agccacaacg aggattatac cgtggttgag caatatgagc 3601 gtagcgaggg tcgccacagc accggcggca tggacgaact gtataaggga tcctaataa // -LOCUS BASIC_SEVA_37_TS.10 3728 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_39.10 3388 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Chloramphenicol resistance marker and - temperature sensitive pSC101 origin of replication.. -ACCESSION BASIC_SEVA_37_TS -VERSION BASIC_SEVA_37_TS.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 193..975 - /label="SEVA_Cm" - misc_feature 988..1040 - /label="BSEVA_L1" - CDS complement(1102..2052) - /label="Rep101(Ts)" - rep_origin 2100..2322 - /label="pSC101 ori" - misc_feature 2592..2696 - /label="SEVA_T1" - misc_feature 2708..2762 - /label="LMS" - misc_feature 2855..2889 - /label="J23119" - misc_feature 3007..3014 - /label="RBS-A12" - CDS 3021..3725 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttaa attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac - 241 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct - 301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg - 361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc - 421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat - 481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca - 541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat - 601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt - 661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa - 721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt - 781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat - 841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt - 901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag - 961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga - 1021 ggtccacaca gatagtgtcc tgttctactt ttgtttgtta gtcttgatgc ttcactgata - 1081 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt - 1141 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat - 1201 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg - 1261 tagtgttttt cttagtccgt tatgtaggta ggaatctgat gtaatggttg ttggtatttt - 1321 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc - 1381 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt - 1441 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct - 1501 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat - 1561 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat - 1621 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt - 1681 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct - 1741 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct - 1801 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt - 1861 ttccctactg atgttcatca tctgaacgta ttggttataa gtgaacgata ccgtccgttc - 1921 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt - 1981 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac - 2041 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg - 2101 ggctagtcaa tgataattac tagtcctttt cctttgagtt gtgggtatct gtaaattctg - 2161 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc - 2221 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata - 2281 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc - 2341 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa - 2401 ccctaaaggc ttaagtagca ccctcgcaag ctcggttgcg gccgcaatcg ggcaaatcgc - 2461 tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt tcgtgacatt - 2521 cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta caggcgcggc - 2581 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca - 2641 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgccttta - 2701 attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact tcggaatcgt - 2761 ccccaattat tgaacaccct tcggggtgtt tttttgtttc tggtctacca tctcgttgtg - 2821 ataatagacc tgaagtgcct actctggaaa atctttgaca gctagctcag tcctaggtat - 2881 aatgctagca gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca - 2941 gcctctacaa ataattttgt ttaaggctcg ctacgagaag attgttactt tcgcagcgtt - 3001 attatctaag gaggtagtcc atggttagca aaggcgaggc ggttatcaag gagtttatgc - 3061 gttttaaggt tcacatggag ggtagcatga atggtcacga gttcgagatc gagggtgaag - 3121 gcgagggtcg tccgtacgaa ggcacccaga ccgcgaagct gaaagtgacc aagggtggcc - 3181 cgctgccgtt cagctgggac atcctgagcc cgcagttcat gtatggcagc cgtgcgttta - 3241 ccaaacaccc ggcggacatt ccggattact ataagcaaag cttcccggaa ggttttaaat - 3301 gggagcgtgt tatgaacttc gaagatggtg gcgcggtgac cgttacccag gacaccagcc - 3361 tggaggatgg caccctgatt tacaaggtga aactgcgtgg caccaacttt ccgccggatg - 3421 gtccggttat gcagaagaaa acgatgggtt gggaagcgag caccgagcgt ctgtatccgg - 3481 aagatggcgt gctgaagggt gatatcaaaa tggcgctgcg tctgaaggac ggtggccgtt - 3541 acctggcgga ttttaagacc acctataaag cgaagaaacc ggtgcaaatg ccgggtgcgt - 3601 acaacgttga ccgtaaactg gatattacca gccacaacga ggattatacc gtggttgagc - 3661 aatatgagcg tagcgagggt cgccacagca ccggcggcat ggacgaactg tataagggat - 3721 cctaataa -// -LOCUS BASIC_SEVA_38.10 2932 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Chloramphenicol resistance marker and - pUC origin of replication.. -ACCESSION BASIC_SEVA_38 -VERSION BASIC_SEVA_38.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 193..975 - /label="SEVA_Cm" - misc_feature 988..1040 - /label="BSEVA_L1" - misc_feature 1049..1977 - /label="SEVA_pUC" - misc_feature 1992..2096 - /label="SEVA_T1" - misc_feature 2108..2162 - /label="LMS" - misc_feature 2163..2197 - /label="J23106" - misc_feature 2206..2217 - /label="B0034" - CDS 2225..2929 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttaa attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac - 241 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct - 301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg - 361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc - 421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat - 481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca - 541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat - 601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt - 661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa - 721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt - 781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat - 841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt - 901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag - 961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga - 1021 ggtccacaca gatagtgtcc ggccggcccc gtagaaaaga tcaaaggatc ttcttgagat - 1081 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg - 1141 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga - 1201 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac - 1261 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt - 1321 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag - 1381 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc - 1441 gaactgagat acctacagcg tgagctttga gaaagcgcca cgcttcccga agggagaaag - 1501 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca - 1561 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt - 1621 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc - 1681 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc - 1741 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc - 1801 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa - 1861 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac - 1921 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaggc - 1981 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca - 2041 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgccttta - 2101 attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact tcggaatcgt - 2161 cctttacggc tagctcagtc ctaggtatag tgctagctac tagtgaaaga ggagaaatac - 2221 tagtatggtt agcaaaggcg aggcggttat caaggagttt atgcgtttta aggttcacat - 2281 ggagggtagc atgaatggtc acgagttcga gatcgagggt gaaggcgagg gtcgtccgta - 2341 cgaaggcacc cagaccgcga agctgaaagt gaccaagggt ggcccgctgc cgttcagctg - 2401 ggacatcctg agcccgcagt tcatgtatgg cagccgtgcg tttaccaaac acccggcgga - 2461 cattccggat tactataagc aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa - 2521 cttcgaagat ggtggcgcgg tgaccgttac ccaggacacc agcctggagg atggcaccct - 2581 gatttacaag gtgaaactgc gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa - 2641 gaaaacgatg ggttgggaag cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa - 2701 gggtgatatc aaaatggcgc tgcgtctgaa ggacggtggc cgttacctgg cggattttaa - 2761 gaccacctat aaagcgaaga aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa - 2821 actggatatt accagccaca acgaggatta taccgtggtt gagcaatatg agcgtagcga - 2881 gggtcgccac agcaccggcg gcatggacga actgtataag ggatcctaat aa -// -LOCUS BASIC_SEVA_39.10 3388 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Chloramphenicol resistance marker and - pBR322-ROP origin of replication.. -ACCESSION BASIC_SEVA_39 -VERSION BASIC_SEVA_39.10 + pBR322-ROP origin of replication. +ACCESSION 927f54a3afd7bab22d2c2cc3e428bf4a +VERSION 927f54a3afd7bab22d2c2cc3e428bf4a KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -1670,7 +1322,7 @@ FEATURES Location/Qualifiers misc_feature 2564..2618 /label="LMS" misc_feature 2619..2653 - /label="J23119" + /label="J23106" misc_feature 2662..2673 /label="B0034" CDS 2681..3385 @@ -1719,8 +1371,8 @@ ORIGIN 2401 agggtaaaca actggcggta tggatgcggc gggggcgcgc ccagctgtct agggcggcgg 2461 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 2521 tttcgactga gcctttcgtt ttatttgatg cctttaatta aggctcggga gacctatcgg - 2581 taataacagt ccaatctggt gtaacttcgg aatcgtcctt gacagctagc tcagtcctag - 2641 gtataatgct agctactagt gaaagaggag aaatactagt atggttagca aaggcgaggc + 2581 taataacagt ccaatctggt gtaacttcgg aatcgtcctt tacggctagc tcagtcctag + 2641 gtatagtgct agctactagt gaaagaggag aaatactagt atggttagca aaggcgaggc 2701 ggttatcaag gagtttatgc gttttaaggt tcacatggag ggtagcatga atggtcacga 2761 gttcgagatc gagggtgaag gcgagggtcg tccgtacgaa ggcacccaga ccgcgaagct 2821 gaaagtgacc aagggtggcc cgctgccgtt cagctgggac atcctgagcc cgcagttcat @@ -1734,11 +1386,108 @@ ORIGIN 3301 ggattatacc gtggttgagc aatatgagcg tagcgagggt cgccacagca ccggcggcat 3361 ggacgaactg tataagggat cctaataa // -LOCUS BASIC_SEVA_45.10 5891 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Streptomycin resistance marker and - RSF1010 origin of replication.. -ACCESSION BASIC_SEVA_45 -VERSION BASIC_SEVA_45.10 +LOCUS BASIC_SEVA_37_pKD46.10 3728 bp DNA circular UNK 03-NOV-2021 +DEFINITION BASIC SEVA vector containing Chloramphenicol resistance marker and + temperature sensitive pSC101 origin of replication. +ACCESSION b89dedf6aac9bef578d7fd8ac362c845 +VERSION b89dedf6aac9bef578d7fd8ac362c845 +KEYWORDS . +SOURCE synthetic construct + ORGANISM synthetic construct + other sequences; artificial sequences. +FEATURES Location/Qualifiers + misc_feature 3..57 + /label="LMP" + misc_feature 64..166 + /label="SEVA_T0" + misc_feature 193..975 + /label="SEVA_Cm" + misc_feature 988..1040 + /label="BSEVA_L1" + CDS 1102..2052 + /label="Rep101(pKD46)" + rep_origin 2100..2322 + /label="pSC101 ori" + misc_feature 2592..2696 + /label="SEVA_T1" + misc_feature 2708..2762 + /label="LMS" + misc_feature 2855..2889 + /label="J23119" + misc_feature 3007..3014 + /label="RBS-A12" + CDS 3021..3725 + /label="mScarlet" +ORIGIN + 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact + 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc + 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat + 181 tacgatttaa attggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac + 241 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct + 301 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg + 361 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc + 421 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat + 481 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca + 541 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat + 601 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt + 661 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa + 721 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt + 781 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat + 841 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt + 901 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag + 961 ggcggggcgt aatttgactt ttgtcggctc ggcccacttg tgtgtctcgt acaattctga + 1021 ggtccacaca gatagtgtcc tgttctactt ttgtttgtta gtcttgatgc ttcactgata + 1081 gatacaagag ccataagaac ctcagatcct tccgtattta gccagtatgt tctctagtgt + 1141 ggttcgttgt ttttgcgtga gccatgagaa cgaaccattg agatcatact tactttgcat + 1201 gtcactcaaa aattttgcct caaaactggt gagctgaatt tttgcagtta aagcatcgtg + 1261 tagtgttttt cttagtccgt tacgtaggta ggaatctgat gtaatggttg ttggtatttt + 1321 gtcaccattc atttttatct ggttgttctc aagttcggtt acgagatcca tttgtctatc + 1381 tagttcaact tggaaaatca acgtatcagt cgggcggcct cgcttatcaa ccaccaattt + 1441 catattgctg taagtgttta aatctttact tattggtttc aaaacccatt ggttaagcct + 1501 tttaaactca tggtagttat tttcaagcat taacatgaac ttaaattcat caaggctaat + 1561 ctctatattt gccttgtgag ttttcttttg tgttagttct tttaataacc actcataaat + 1621 cctcatagag tatttgtttt caaaagactt aacatgttcc agattatatt ttatgaattt + 1681 ttttaactgg aaaagataag gcaatatctc ttcactaaaa actaattcta atttttcgct + 1741 tgagaacttg gcatagtttg tccactggaa aatctcaaag cctttaacca aaggattcct + 1801 gatttccaca gttctcgtca tcagctctct ggttgcttta gctaatacac cataagcatt + 1861 ttccctactg atgttcatca tctgagcgta ttggttataa gtgaacgata ccgtccgttc + 1921 tttccttgta gggttttcaa tcgtggggtt gagtagtgcc acacagcata aaattagctt + 1981 ggtttcatgc tccgttaagt catagcgact aatcgctagt tcatttgctt tgaaaacaac + 2041 taattcagac atacatctca attggtctag gtgattttaa tcactatacc aattgagatg + 2101 ggctagtcaa tgataattac tagtcctttt cctttgagtt gtgggtatct gtaaattctg + 2161 ctagaccttt gctggaaaac ttgtaaattc tgctagaccc tctgtaaatt ccgctagacc + 2221 tttgtgtgtt ttttttgttt atattcaagt ggttataatt tatagaataa agaaagaata + 2281 aaaaaagata aaaagaatag atcccagccc tgtgtataac tcactacttt agtcagttcc + 2341 gcagtattac aaaaggatgt cgcaaacgct gtttgctcct ctacaaaaca gaccttaaaa + 2401 ccctaaaggc ttaagtagca ccctcgcaag ctcggttgcg gccgcaatcg ggcaaatcgc + 2461 tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt tcgtgacatt + 2521 cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta caggcgcggc + 2581 gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca ccgacaaaca + 2641 acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgccttta + 2701 attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact tcggaatcgt + 2761 ccccaattat tgaacaccct tcggggtgtt tttttgtttc tggtctacca tctcgttgtg + 2821 ataatagacc tgaagtgcct actctggaaa atctttgaca gctagctcag tcctaggtat + 2881 aatgctagca gctgtcaccg gatgtgcttt ccggtctgat gagtccgtga ggacgaaaca + 2941 gcctctacaa ataattttgt ttaaggctcg ctacgagaag attgttactt tcgcagcgtt + 3001 attatctaag gaggtagtcc atggttagca aaggcgaggc ggttatcaag gagtttatgc + 3061 gttttaaggt tcacatggag ggtagcatga atggtcacga gttcgagatc gagggtgaag + 3121 gcgagggtcg tccgtacgaa ggcacccaga ccgcgaagct gaaagtgacc aagggtggcc + 3181 cgctgccgtt cagctgggac atcctgagcc cgcagttcat gtatggcagc cgtgcgttta + 3241 ccaaacaccc ggcggacatt ccggattact ataagcaaag cttcccggaa ggttttaaat + 3301 gggagcgtgt tatgaacttc gaagatggtg gcgcggtgac cgttacccag gacaccagcc + 3361 tggaggatgg caccctgatt tacaaggtga aactgcgtgg caccaacttt ccgccggatg + 3421 gtccggttat gcagaagaaa acgatgggtt gggaagcgag caccgagcgt ctgtatccgg + 3481 aagatggcgt gctgaagggt gatatcaaaa tggcgctgcg tctgaaggac ggtggccgtt + 3541 acctggcgga ttttaagacc acctataaag cgaagaaacc ggtgcaaatg ccgggtgcgt + 3601 acaacgttga ccgtaaactg gatattacca gccacaacga ggattatacc gtggttgagc + 3661 aatatgagcg tagcgagggt cgccacagca ccggcggcat ggacgaactg tataagggat + 3721 cctaataa +// +LOCUS BASIC_SEVA_45a.10 5891 bp DNA circular UNK 03-NOV-2021 +DEFINITION BASIC SEVA vector containing Streptomycin/Spectinomycin resistance + marker and RSF1010 origin of replication. +ACCESSION 56da3cbe26311835d21578bba476ebad +VERSION 56da3cbe26311835d21578bba476ebad KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -1752,8 +1501,8 @@ FEATURES Location/Qualifiers /label="SEVA_Sm/Sp" misc_feature 1202..1254 /label="BSEVA_L1" - misc_feature 1263..4936 - /label="SEVA_RSF101" + misc_feature 1266..4936 + /label="SEVA_RSF1010(x5ax)" misc_feature 4951..5055 /label="SEVA_T1" misc_feature 5067..5121 @@ -1785,61 +1534,61 @@ ORIGIN 1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag 1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga 1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg - 1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg - 1261 cctcagcctg ccgccttggg ccgggtgatg tcgtacttgc ccgccgcgaa ctcggttacc - 1321 gtccagccca gcgcgaccag ctccggcaac gcctcgcgca cccgctggcg gcgcttgcgc - 1381 atggtcgaac cactggcctc tgacggccag acatagccgc acaaggtatc tatggaagcc - 1441 ttgccggttt tgccggggtc gatccagcca cacagccgct ggtgcagcag gcgggcggtt - 1501 tcgctgtcca gcgcccgcac ctcgtccatg ctgatgcgca catgctggcc gccacccatg - 1561 acggcctgcg cgatcaaggg gttcagggcc acgtacaggc gcccgtccgc ctcgtcgctg - 1621 gcgtactccg acagcagccg aaacccctgc cgcttgcggc cattctgggc gatgatggat - 1681 accttccaaa ggcgctcgat gcagtcctgt atgtgcttga gcgccccacc actatcgacc - 1741 tctgccccga tttcctttgc cagcgcccga tagctacctt tgaccacatg gcattcagcg - 1801 gtgacggcct cccacttggg ttccaggaac agccggagct gccgtccgcc ttcggtcttg - 1861 ggttccgggc caagcactag gccattaggc ccagccatgg ccaccagccc ttgcaggatg - 1921 cgcagatcat cagcgcccag cggctccggg ccgctgaact cgatccgctt gccgtcgccg - 1981 tagtcatacg tcacgtccag cttgctgcgc ttgcgctcgc cccgcttgag ggcacggaac - 2041 aggccggggg ccagacagtg cgccgggtcg tgccggacgt ggctgaggct gtgcttgttc - 2101 ttaggcttca ccacggggca cccccttgct cttgcgctgc ctctccagca cggcgggctt - 2161 gagcaccccg ccgtcatgcc gcctgaacca ccgatcagcg aacggtgcgc catagttggc - 2221 cttgctcaca ccgaagcgga cgaagaaccg gcgctggtcg tcgtccacac cccattcctc - 2281 ggcctcggcg ctggtcatgc tcgacaggta ggactgccag cggatgttat cgaccagtac - 2341 cgagctgccc cggctggcct gctgctggtc gcctgcgccc atcatggccg cgcccttgct - 2401 ggcatggtgc aggaacacga tagagcaccc ggtatcggcg gcgatggcct ccatgcgacc - 2461 gatgacctgg gccatggggc cgctggcgtt ttcttcctcg atgtggaacc ggcgcagcgt - 2521 gtccagcacc atcaggcggc ggccctcggc ggcgcgcttg aggccgtcga accactccgg - 2581 ggccatgatg ttgggcaggc tgccgatcag cggctggatc agcaggccgt cagccacggc - 2641 ttgccgttcc tcggcgctga ggtgcgcccc aagggcgtgc aggcggtgat gaatggcggt - 2701 gggcgggtct tcggcgggca ggtagatcac cgggccggtg ggcagttcgc ccacctccag - 2761 cagatccggc ccgcctgcaa tctgtgcggc cagttgcagg gccagcatgg atttaccggc - 2821 accaccgggc gacaccagcg ccccgaccgt accggccacc atgttgggca aaacgtagtc - 2881 cagcggtggc ggcgctgctg cgaacgcctc cagaatattg ataggcttat gggtagccat - 2941 tgattgcctc ctttgcaggc agttggtggt taggcgctgg cggggtcact acccccgccc - 3001 tgcgccgctc tgagttcttc caggcactcg cgcagcgcct cgtattcgtc gtcggtcagc - 3061 cagaacttgc gctgacgcat ccctttggcc ttcatgcgct cggcatatcg cgcttggcgt - 3121 acagcgtcag ggctggccag caggtcgccg gtctgcttgt ccttttggtc tttcatatca - 3181 gtcaccgaga aacttgccgg ggccgaaagg cttgtcttcg cggaacaagg acaaggtgca - 3241 gccgtcaagg ttaaggctgg ccatatcagc gactgaaaag cggccagcct cggccttgtt - 3301 tgacgtataa ccaaagccac cgggcaacca atagcccttg tcacttttga tcaggtagac - 3361 cgaccctgaa gcgctttttt cgtattccat aaaaccccct tctgtgcgtg agtactcata - 3421 gtataacagg cgtgagtacc aacgcaagca ctacatgctg aaatctggcc cgcccctgtc - 3481 catgcctcgc tggcggggtg ccggtgcccg tgccagctcg gcccgcgcaa gctggacgct - 3541 gggcagaccc atgaccttgc tgacggtgcg ctcgatgtaa tccgcttcgt ggccgggctt - 3601 gcgctctgcc agcgctgggc tggcctcggc catggccttg ccgatttcct cggcactgcg - 3661 gccccggctg gccagcttct gcgcggcgat aaagtcgcac ttgctgaggt catcaccgaa - 3721 gcgcttgacc agcccggcca tctcgctgcg gtactcgtcc agcgccgtgc gccggtggcg - 3781 gctaagctgc cgctcgggca gttcgaggct ggccagcctg cgggccttct cctgctgccg - 3841 ctgggcctgc tcgatctgct ggccagcctg ctgcaccagc gccgggccag cggtggcggt - 3901 cttgcccttg gattcacgca gcagcaccca cggctgataa ccggcgcggg tggtgtgctt - 3961 gtccttgcgg ttggtgaagc ccgccaagcg gccatagtgg cggctgtcgg cgctggccgg - 4021 gtcggcgtcg tactcgctgg ccagcgtccg ggcaatctgc ccccgaagtt caccgcctgc - 4081 ggcgtcggcc accttgaccc atgcctgata gttcttcggg ctggtttcca ctaccagggc - 4141 aggctcccgg ccctcggctt tcatgtcatc caggtcaaac tcgctgaggt cgtccaccag - 4201 caccagacca tgccgctcct gctcggcggg cctgatatac acgtcattgc cctgggcatt - 4261 catccgcttg agccatggcg tgttctggag cacttcggcg gctgaccatt cccggttcat - 4321 catctggccg gtggtggcgt ccctgacgcc gatatcgaag cgctcacagc ccatggcctt - 4381 gagctgtcgg cctatggcct gcaaagtcct gtcgttcttc atcgggccac caagcgattc - 4441 ccacacatta tacgagccgg aagcataaag tgtaaagcct agatccgaag gatgagccgg + 1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccgtaggc + 1261 cggcctcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt + 1321 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg + 1381 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa + 1441 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg + 1501 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc + 1561 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg + 1621 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg + 1681 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg + 1741 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac catggcatca + 1801 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc + 1861 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg + 1921 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg + 1981 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg + 2041 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg + 2101 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg + 2161 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt + 2221 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc + 2281 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag + 2341 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt + 2401 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg + 2461 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag + 2521 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc + 2581 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac + 2641 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc + 2701 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc + 2761 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc + 2821 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta + 2881 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc + 2941 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg + 3001 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc + 3061 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg + 3121 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata + 3181 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt + 3241 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt + 3301 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta + 3361 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc + 3421 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct + 3481 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac + 3541 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg + 3601 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact + 3661 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc + 3721 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg + 3781 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg + 3841 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc + 3901 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg + 3961 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc + 4021 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc + 4081 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag + 4141 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac + 4201 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc + 4261 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt + 4321 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc + 4381 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga + 4441 ttcccacaca ttatacgagc cggaagcata aagtgtagct agatccgaag gatgagccgg 4501 gctgaatgat cgaccgagac aggccctgcg gggctgcaca cgcgccccca cccttcgggt 4561 agggggaaag gccgctaaag cggctaaaag cgctccagcg tatttctgcg gggtttggtg 4621 tggggtttag cgggctttgc ccgcctttcc ccctgccgcg cagcggtggg gcggtgtgta @@ -1865,11 +1614,11 @@ ORIGIN 5821 agcaatatga gcgtagcgag ggtcgccaca gcaccggcgg catggacgaa ctgtataagg 5881 gatcctaata a // -LOCUS BASIC_SEVA_46.10 2949 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Streptomycin resistance marker and p15A - origin of replication.. -ACCESSION BASIC_SEVA_46 -VERSION BASIC_SEVA_46.10 +LOCUS BASIC_SEVA_46.10 2949 bp DNA circular UNK 03-NOV-2021 +DEFINITION BASIC SEVA vector containing Streptomycin/Spectinomycin resistance + marker and p15A origin of replication. +ACCESSION 4d797ce1665dcb6dc7e73376928424e7 +VERSION 4d797ce1665dcb6dc7e73376928424e7 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -1947,11 +1696,11 @@ ORIGIN 2881 caatatgagc gtagcgaggg tcgccacagc accggcggca tggacgaact gtataaggga 2941 tcctaataa // -LOCUS BASIC_SEVA_47.10 3873 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Streptomycin resistance marker and - pSC101 origin of replication.. -ACCESSION BASIC_SEVA_47 -VERSION BASIC_SEVA_47.10 +LOCUS BASIC_SEVA_47.10 3873 bp DNA circular UNK 03-NOV-2021 +DEFINITION BASIC SEVA vector containing Streptomycin/Spectinomycin resistance + marker and pSC101 origin of replication. +ACCESSION b23db40e89aa7f017b096e4158e729bc +VERSION b23db40e89aa7f017b096e4158e729bc KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -2044,196 +1793,11 @@ ORIGIN 3781 aacgaggatt ataccgtggt tgagcaatat gagcgtagcg agggtcgcca cagcaccggc 3841 ggcatggacg aactgtataa gggatcctaa taa // -LOCUS BASIC_SEVA_47_TS.10 3942 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Streptomycin resistance marker and - temperature sensitive pSC101 origin of replication.. -ACCESSION BASIC_SEVA_47_TS -VERSION BASIC_SEVA_47_TS.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 201..1189 - /label="SEVA_Sm/Sp" - misc_feature 1202..1254 - /label="BSEVA_L1" - CDS complement(1316..2266) - /label="Rep101(Ts)" - rep_origin 2314..2536 - /label="pSC101 ori" - misc_feature 2806..2910 - /label="SEVA_T1" - misc_feature 2922..2976 - /label="LMS" - misc_feature 3069..3103 - /label="J23119" - misc_feature 3221..3228 - /label="RBS-A12" - CDS 3235..3939 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttac gtatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc - 241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa - 301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac - 361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga - 421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt - 481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat - 541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa - 601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt - 661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca - 721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga - 781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc - 841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga - 901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct - 961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc - 1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag - 1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga - 1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg - 1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtcctgttct - 1261 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga - 1321 tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg - 1381 agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac - 1441 tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttatgta - 1501 ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt - 1561 tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat - 1621 cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt - 1681 tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa - 1741 gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct - 1801 tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag - 1861 acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata - 1921 tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact - 1981 ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct - 2041 ctctggttgc tttagctaat acaccataag cattttccct actgatgttc atcatctgaa - 2101 cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg - 2161 ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc - 2221 gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt - 2281 ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc - 2341 ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa - 2401 attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc - 2461 aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca - 2521 gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa - 2581 cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg - 2641 caagctcggt tgcggccgca atcgggcaaa tcgctgaata ttccttttgt ctccgaccat - 2701 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc - 2761 agtgaatggg ggtaaatggc actacaggcg cggcgcgccc agctgtctag ggcggcggat - 2821 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt - 2881 tcgactgagc ctttcgtttt atttgatgcc tttaattaag gctcgggaga cctatcggta - 2941 ataacagtcc aatctggtgt aacttcggaa tcgtccccaa ttattgaaca cccttcgggg - 3001 tgtttttttg tttctggtct accatctcgt tgtgataata gacctgaagt gcctactctg - 3061 gaaaatcttt gacagctagc tcagtcctag gtataatgct agcagctgtc accggatgtg - 3121 ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaagg - 3181 ctcgctacga gaagattgtt actttcgcag cgttattatc taaggaggta gtccatggtt - 3241 agcaaaggcg aggcggttat caaggagttt atgcgtttta aggttcacat ggagggtagc - 3301 atgaatggtc acgagttcga gatcgagggt gaaggcgagg gtcgtccgta cgaaggcacc - 3361 cagaccgcga agctgaaagt gaccaagggt ggcccgctgc cgttcagctg ggacatcctg - 3421 agcccgcagt tcatgtatgg cagccgtgcg tttaccaaac acccggcgga cattccggat - 3481 tactataagc aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa cttcgaagat - 3541 ggtggcgcgg tgaccgttac ccaggacacc agcctggagg atggcaccct gatttacaag - 3601 gtgaaactgc gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa gaaaacgatg - 3661 ggttgggaag cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa gggtgatatc - 3721 aaaatggcgc tgcgtctgaa ggacggtggc cgttacctgg cggattttaa gaccacctat - 3781 aaagcgaaga aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa actggatatt - 3841 accagccaca acgaggatta taccgtggtt gagcaatatg agcgtagcga gggtcgccac - 3901 agcaccggcg gcatggacga actgtataag ggatcctaat aa -// -LOCUS BASIC_SEVA_48.10 3146 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Streptomycin resistance marker and pUC - origin of replication.. -ACCESSION BASIC_SEVA_48 -VERSION BASIC_SEVA_48.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 201..1189 - /label="SEVA_Sm/Sp" - misc_feature 1202..1254 - /label="BSEVA_L1" - misc_feature 1263..2191 - /label="SEVA_pUC" - misc_feature 2206..2310 - /label="SEVA_T1" - misc_feature 2322..2376 - /label="LMS" - misc_feature 2377..2411 - /label="J23106" - misc_feature 2420..2431 - /label="B0034" - CDS 2439..3143 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttac gtatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc - 241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa - 301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac - 361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga - 421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt - 481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat - 541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa - 601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt - 661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca - 721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga - 781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc - 841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga - 901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct - 961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc - 1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag - 1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga - 1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg - 1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtccggccgg - 1261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg - 1321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc - 1381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct - 1441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc - 1501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt - 1561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg - 1621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct - 1681 ttgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag - 1741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag - 1801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg - 1861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg - 1921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac - 1981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt - 2041 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat - 2101 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc - 2161 aattaatgtg agttagctca ctcattaggc aggcgcgccc agctgtctag ggcggcggat - 2221 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt - 2281 tcgactgagc ctttcgtttt atttgatgcc tttaattaag gctcgggaga cctatcggta - 2341 ataacagtcc aatctggtgt aacttcggaa tcgtccttta cggctagctc agtcctaggt - 2401 atagtgctag ctactagtga aagaggagaa atactagtat ggttagcaaa ggcgaggcgg - 2461 ttatcaagga gtttatgcgt tttaaggttc acatggaggg tagcatgaat ggtcacgagt - 2521 tcgagatcga gggtgaaggc gagggtcgtc cgtacgaagg cacccagacc gcgaagctga - 2581 aagtgaccaa gggtggcccg ctgccgttca gctgggacat cctgagcccg cagttcatgt - 2641 atggcagccg tgcgtttacc aaacacccgg cggacattcc ggattactat aagcaaagct - 2701 tcccggaagg ttttaaatgg gagcgtgtta tgaacttcga agatggtggc gcggtgaccg - 2761 ttacccagga caccagcctg gaggatggca ccctgattta caaggtgaaa ctgcgtggca - 2821 ccaactttcc gccggatggt ccggttatgc agaagaaaac gatgggttgg gaagcgagca - 2881 ccgagcgtct gtatccggaa gatggcgtgc tgaagggtga tatcaaaatg gcgctgcgtc - 2941 tgaaggacgg tggccgttac ctggcggatt ttaagaccac ctataaagcg aagaaaccgg - 3001 tgcaaatgcc gggtgcgtac aacgttgacc gtaaactgga tattaccagc cacaacgagg - 3061 attataccgt ggttgagcaa tatgagcgta gcgagggtcg ccacagcacc ggcggcatgg - 3121 acgaactgta taagggatcc taataa -// -LOCUS BASIC_SEVA_49.10 3602 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Streptomycin resistance marker and - pBR322-ROP origin of replication.. -ACCESSION BASIC_SEVA_49 -VERSION BASIC_SEVA_49.10 +LOCUS BASIC_SEVA_49.10 3602 bp DNA circular UNK 03-NOV-2021 +DEFINITION BASIC SEVA vector containing Streptomycin/Spectinomycin resistance + marker and pBR322-ROP origin of replication. +ACCESSION 804fbb49400cf5027a668feb1ec60c06 +VERSION 804fbb49400cf5027a668feb1ec60c06 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -2254,7 +1818,7 @@ FEATURES Location/Qualifiers misc_feature 2778..2832 /label="LMS" misc_feature 2833..2867 - /label="J23119" + /label="J23106" misc_feature 2876..2887 /label="B0034" CDS 2895..3599 @@ -2307,7 +1871,7 @@ ORIGIN 2641 cggcgggggc gcgcccagct gtctagggcg gcggatttgt cctactcagg agagcgttca 2701 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 2761 gatgccttta attaaggctc gggagaccta tcggtaataa cagtccaatc tggtgtaact - 2821 tcggaatcgt ccttgacagc tagctcagtc ctaggtataa tgctagctac tagtgaaaga + 2821 tcggaatcgt cctttacggc tagctcagtc ctaggtatag tgctagctac tagtgaaaga 2881 ggagaaatac tagtatggtt agcaaaggcg aggcggttat caaggagttt atgcgtttta 2941 aggttcacat ggagggtagc atgaatggtc acgagttcga gatcgagggt gaaggcgagg 3001 gtcgtccgta cgaaggcacc cagaccgcga agctgaaagt gaccaagggt ggcccgctgc @@ -2322,11 +1886,111 @@ ORIGIN 3541 agcgtagcga gggtcgccac agcaccggcg gcatggacga actgtataag ggatcctaat 3601 aa // -LOCUS BASIC_SEVA_55.10 6169 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_47_pKD46.10 3942 bp DNA circular UNK 03-NOV-2021 +DEFINITION BASIC SEVA vector containing Streptomycin/Spectinomycin resistance + marker and temperature sensitive pSC101 origin of replication. +ACCESSION a388b01c3624b66b0f4f70447ef4b42c +VERSION a388b01c3624b66b0f4f70447ef4b42c +KEYWORDS . +SOURCE synthetic construct + ORGANISM synthetic construct + other sequences; artificial sequences. +FEATURES Location/Qualifiers + misc_feature 3..57 + /label="LMP" + misc_feature 64..166 + /label="SEVA_T0" + misc_feature 201..1189 + /label="SEVA_Sm/Sp" + misc_feature 1202..1254 + /label="BSEVA_L1" + CDS 1316..2266 + /label="Rep101(pKD46)" + rep_origin 2314..2536 + /label="pSC101 ori" + misc_feature 2806..2910 + /label="SEVA_T1" + misc_feature 2922..2976 + /label="LMS" + misc_feature 3069..3103 + /label="J23119" + misc_feature 3221..3228 + /label="RBS-A12" + CDS 3235..3939 + /label="mScarlet" +ORIGIN + 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact + 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc + 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat + 181 tacgatttac gtatttaaat gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc + 241 ggttttcatg gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa + 301 gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac + 361 gcagcagggc agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga + 421 agtatcgact caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt + 481 gctggccgta catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat + 541 tgatttgctg gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa + 601 cgaccttttg gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt + 661 caccattgtt gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca + 721 atttggagaa tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga + 781 cattgatctg gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc + 841 agcggcggag gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga + 901 aaccttaacg ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct + 961 tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc + 1021 tgccgactgg gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag + 1081 acaggcttat cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga + 1141 atttgtccac tacgtgaaag gcgagatcac caaggtagtc ggcaaataag acaattgtcg + 1201 gctcggccca cttgtgtgtc tcgtacaatt ctgaggtcca cacagatagt gtcctgttct + 1261 acttttgttt gttagtcttg atgcttcact gatagataca agagccataa gaacctcaga + 1321 tccttccgta tttagccagt atgttctcta gtgtggttcg ttgtttttgc gtgagccatg + 1381 agaacgaacc attgagatca tacttacttt gcatgtcact caaaaatttt gcctcaaaac + 1441 tggtgagctg aatttttgca gttaaagcat cgtgtagtgt ttttcttagt ccgttacgta + 1501 ggtaggaatc tgatgtaatg gttgttggta ttttgtcacc attcattttt atctggttgt + 1561 tctcaagttc ggttacgaga tccatttgtc tatctagttc aacttggaaa atcaacgtat + 1621 cagtcgggcg gcctcgctta tcaaccacca atttcatatt gctgtaagtg tttaaatctt + 1681 tacttattgg tttcaaaacc cattggttaa gccttttaaa ctcatggtag ttattttcaa + 1741 gcattaacat gaacttaaat tcatcaaggc taatctctat atttgccttg tgagttttct + 1801 tttgtgttag ttcttttaat aaccactcat aaatcctcat agagtatttg ttttcaaaag + 1861 acttaacatg ttccagatta tattttatga atttttttaa ctggaaaaga taaggcaata + 1921 tctcttcact aaaaactaat tctaattttt cgcttgagaa cttggcatag tttgtccact + 1981 ggaaaatctc aaagccttta accaaaggat tcctgatttc cacagttctc gtcatcagct + 2041 ctctggttgc tttagctaat acaccataag cattttccct actgatgttc atcatctgag + 2101 cgtattggtt ataagtgaac gataccgtcc gttctttcct tgtagggttt tcaatcgtgg + 2161 ggttgagtag tgccacacag cataaaatta gcttggtttc atgctccgtt aagtcatagc + 2221 gactaatcgc tagttcattt gctttgaaaa caactaattc agacatacat ctcaattggt + 2281 ctaggtgatt ttaatcacta taccaattga gatgggctag tcaatgataa ttactagtcc + 2341 ttttcctttg agttgtgggt atctgtaaat tctgctagac ctttgctgga aaacttgtaa + 2401 attctgctag accctctgta aattccgcta gacctttgtg tgtttttttt gtttatattc + 2461 aagtggttat aatttataga ataaagaaag aataaaaaaa gataaaaaga atagatccca + 2521 gccctgtgta taactcacta ctttagtcag ttccgcagta ttacaaaagg atgtcgcaaa + 2581 cgctgtttgc tcctctacaa aacagacctt aaaaccctaa aggcttaagt agcaccctcg + 2641 caagctcggt tgcggccgca atcgggcaaa tcgctgaata ttccttttgt ctccgaccat + 2701 caggcacctg agtcgctgtc tttttcgtga cattcagttc gctgcgctca cggctctggc + 2761 agtgaatggg ggtaaatggc actacaggcg cggcgcgccc agctgtctag ggcggcggat + 2821 ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt + 2881 tcgactgagc ctttcgtttt atttgatgcc tttaattaag gctcgggaga cctatcggta + 2941 ataacagtcc aatctggtgt aacttcggaa tcgtccccaa ttattgaaca cccttcgggg + 3001 tgtttttttg tttctggtct accatctcgt tgtgataata gacctgaagt gcctactctg + 3061 gaaaatcttt gacagctagc tcagtcctag gtataatgct agcagctgtc accggatgtg + 3121 ctttccggtc tgatgagtcc gtgaggacga aacagcctct acaaataatt ttgtttaagg + 3181 ctcgctacga gaagattgtt actttcgcag cgttattatc taaggaggta gtccatggtt + 3241 agcaaaggcg aggcggttat caaggagttt atgcgtttta aggttcacat ggagggtagc + 3301 atgaatggtc acgagttcga gatcgagggt gaaggcgagg gtcgtccgta cgaaggcacc + 3361 cagaccgcga agctgaaagt gaccaagggt ggcccgctgc cgttcagctg ggacatcctg + 3421 agcccgcagt tcatgtatgg cagccgtgcg tttaccaaac acccggcgga cattccggat + 3481 tactataagc aaagcttccc ggaaggtttt aaatgggagc gtgttatgaa cttcgaagat + 3541 ggtggcgcgg tgaccgttac ccaggacacc agcctggagg atggcaccct gatttacaag + 3601 gtgaaactgc gtggcaccaa ctttccgccg gatggtccgg ttatgcagaa gaaaacgatg + 3661 ggttgggaag cgagcaccga gcgtctgtat ccggaagatg gcgtgctgaa gggtgatatc + 3721 aaaatggcgc tgcgtctgaa ggacggtggc cgttacctgg cggattttaa gaccacctat + 3781 aaagcgaaga aaccggtgca aatgccgggt gcgtacaacg ttgaccgtaa actggatatt + 3841 accagccaca acgaggatta taccgtggtt gagcaatatg agcgtagcga gggtcgccac + 3901 agcaccggcg gcatggacga actgtataag ggatcctaat aa +// +LOCUS BASIC_SEVA_5a5a.10 6169 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Tetracycline resistance marker and - RSF1010 origin of replication.. -ACCESSION BASIC_SEVA_55 -VERSION BASIC_SEVA_55.10 + RSF1010 origin of replication. +ACCESSION 4dc7813eba6298720b8e47f618aa2a4b +VERSION 4dc7813eba6298720b8e47f618aa2a4b KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -2337,11 +2001,11 @@ FEATURES Location/Qualifiers misc_feature 64..166 /label="SEVA_T0" misc_feature 201..1467 - /label="SEVA_Tet" + /label="SEVA_Tet(5axx)" misc_feature 1480..1532 /label="BSEVA_L1" - misc_feature 1541..5214 - /label="SEVA_RSF101" + misc_feature 1544..5214 + /label="SEVA_RSF1010(x5ax)" misc_feature 5229..5333 /label="SEVA_T1" misc_feature 5345..5399 @@ -2369,7 +2033,7 @@ ORIGIN 781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg - 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc + 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc 1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg 1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt @@ -2378,61 +2042,61 @@ ORIGIN 1321 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg 1381 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt 1441 gcatggagcc gggccacctc gacctgagac aattgtcggc tcggcccact tgtgtgtctc - 1501 gtacaattct gaggtccaca cagatagtgt ccggccggcc tcagcctgcc gccttgggcc - 1561 gggtgatgtc gtacttgccc gccgcgaact cggttaccgt ccagcccagc gcgaccagct - 1621 ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca ctggcctctg - 1681 acggccagac atagccgcac aaggtatcta tggaagcctt gccggttttg ccggggtcga - 1741 tccagccaca cagccgctgg tgcagcaggc gggcggtttc gctgtccagc gcccgcacct - 1801 cgtccatgct gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg atcaaggggt - 1861 tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac agcagccgaa - 1921 acccctgccg cttgcggcca ttctgggcga tgatggatac cttccaaagg cgctcgatgc - 1981 agtcctgtat gtgcttgagc gccccaccac tatcgacctc tgccccgatt tcctttgcca - 2041 gcgcccgata gctacctttg accacatggc attcagcggt gacggcctcc cacttgggtt - 2101 ccaggaacag ccggagctgc cgtccgcctt cggtcttggg ttccgggcca agcactaggc - 2161 cattaggccc agccatggcc accagccctt gcaggatgcg cagatcatca gcgcccagcg - 2221 gctccgggcc gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc acgtccagct - 2281 tgctgcgctt gcgctcgccc cgcttgaggg cacggaacag gccgggggcc agacagtgcg - 2341 ccgggtcgtg ccggacgtgg ctgaggctgt gcttgttctt aggcttcacc acggggcacc - 2401 cccttgctct tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc gtcatgccgc - 2461 ctgaaccacc gatcagcgaa cggtgcgcca tagttggcct tgctcacacc gaagcggacg - 2521 aagaaccggc gctggtcgtc gtccacaccc cattcctcgg cctcggcgct ggtcatgctc - 2581 gacaggtagg actgccagcg gatgttatcg accagtaccg agctgccccg gctggcctgc - 2641 tgctggtcgc ctgcgcccat catggccgcg cccttgctgg catggtgcag gaacacgata - 2701 gagcacccgg tatcggcggc gatggcctcc atgcgaccga tgacctgggc catggggccg - 2761 ctggcgtttt cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat caggcggcgg - 2821 ccctcggcgg cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt gggcaggctg - 2881 ccgatcagcg gctggatcag caggccgtca gccacggctt gccgttcctc ggcgctgagg - 2941 tgcgccccaa gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc ggcgggcagg - 3001 tagatcaccg ggccggtggg cagttcgccc acctccagca gatccggccc gcctgcaatc - 3061 tgtgcggcca gttgcagggc cagcatggat ttaccggcac caccgggcga caccagcgcc - 3121 ccgaccgtac cggccaccat gttgggcaaa acgtagtcca gcggtggcgg cgctgctgcg - 3181 aacgcctcca gaatattgat aggcttatgg gtagccattg attgcctcct ttgcaggcag - 3241 ttggtggtta ggcgctggcg gggtcactac ccccgccctg cgccgctctg agttcttcca - 3301 ggcactcgcg cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc tgacgcatcc - 3361 ctttggcctt catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg ctggccagca - 3421 ggtcgccggt ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa cttgccgggg - 3481 ccgaaaggct tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt aaggctggcc - 3541 atatcagcga ctgaaaagcg gccagcctcg gccttgtttg acgtataacc aaagccaccg - 3601 ggcaaccaat agcccttgtc acttttgatc aggtagaccg accctgaagc gcttttttcg - 3661 tattccataa aacccccttc tgtgcgtgag tactcatagt ataacaggcg tgagtaccaa - 3721 cgcaagcact acatgctgaa atctggcccg cccctgtcca tgcctcgctg gcggggtgcc - 3781 ggtgcccgtg ccagctcggc ccgcgcaagc tggacgctgg gcagacccat gaccttgctg - 3841 acggtgcgct cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag cgctgggctg - 3901 gcctcggcca tggccttgcc gatttcctcg gcactgcggc cccggctggc cagcttctgc - 3961 gcggcgataa agtcgcactt gctgaggtca tcaccgaagc gcttgaccag cccggccatc - 4021 tcgctgcggt actcgtccag cgccgtgcgc cggtggcggc taagctgccg ctcgggcagt - 4081 tcgaggctgg ccagcctgcg ggccttctcc tgctgccgct gggcctgctc gatctgctgg - 4141 ccagcctgct gcaccagcgc cgggccagcg gtggcggtct tgcccttgga ttcacgcagc - 4201 agcacccacg gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt ggtgaagccc - 4261 gccaagcggc catagtggcg gctgtcggcg ctggccgggt cggcgtcgta ctcgctggcc - 4321 agcgtccggg caatctgccc ccgaagttca ccgcctgcgg cgtcggccac cttgacccat - 4381 gcctgatagt tcttcgggct ggtttccact accagggcag gctcccggcc ctcggctttc - 4441 atgtcatcca ggtcaaactc gctgaggtcg tccaccagca ccagaccatg ccgctcctgc - 4501 tcggcgggcc tgatatacac gtcattgccc tgggcattca tccgcttgag ccatggcgtg - 4561 ttctggagca cttcggcggc tgaccattcc cggttcatca tctggccggt ggtggcgtcc - 4621 ctgacgccga tatcgaagcg ctcacagccc atggccttga gctgtcggcc tatggcctgc - 4681 aaagtcctgt cgttcttcat cgggccacca agcgattccc acacattata cgagccggaa - 4741 gcataaagtg taaagcctag atccgaagga tgagccgggc tgaatgatcg accgagacag + 1501 gtacaattct gaggtccaca cagatagtgt ccgtaggccg gcctcagcct gccgccttgg + 1561 gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc agcgcgacca + 1621 gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa ccactggcct + 1681 ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt ttgccggggt + 1741 cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc agcgcccgca + 1801 cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc gcgatcaagg + 1861 ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc gacagcagcc + 1921 gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa aggcgctcga + 1981 tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg atttcctttg + 2041 ccagcgcccg atagctacct ttgaccacca tggcatcagc ggtgacggcc tcccacttgg + 2101 gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg ccaagcacta + 2161 ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca tcagcgccca + 2221 gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac gtcacgtcca + 2281 gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg gccagacagt + 2341 gcgccgggtc gtgccggacg tggctgaggc tgtgcttgtt cttaggcttc accacggggc + 2401 acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc gccgtcatgc + 2461 cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac accgaagcgg + 2521 acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc gctggtcatg + 2581 ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc ccggctggcc + 2641 tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg caggaacacg + 2701 atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg ggccatgggg + 2761 ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac catcaggcgg + 2821 cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat gttgggcagg + 2881 ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc ctcggcgctg + 2941 aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc ttcggcgggc + 3001 aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg cccgcctgca + 3061 atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg cgacaccagc + 3121 gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg cggcgctgct + 3181 gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct cctttgcagg + 3241 cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct ctgagttctt + 3301 ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg cgctgacgca + 3361 tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca gggctggcca + 3421 gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag aaacttgccg + 3481 gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag gttaaggctg + 3541 gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata accaaagcca + 3601 ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga agcgcttttt + 3661 tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag gcgtgagtac + 3721 caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg ctggcggggt + 3781 gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc catgaccttg + 3841 ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc cagcgctggg + 3901 ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct ggccagcttc + 3961 tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac cagcccggcc + 4021 atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg ccgctcgggc + 4081 agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg ctcgatctgc + 4141 tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt ggattcacgc + 4201 agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg gttggtgaag + 4261 cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc gtactcgctg + 4321 gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc caccttgacc + 4381 catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg gccctcggct + 4441 ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc atgccgctcc + 4501 tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt gagccatggc + 4561 gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc ggtggtggcg + 4621 tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg gcctatggcc + 4681 tgcaaagtcc tgtcgttctt catcgggcca ccaagcgatt cccacacatt atacgagccg + 4741 gaagcataaa gtgtagctag atccgaagga tgagccgggc tgaatgatcg accgagacag 4801 gccctgcggg gctgcacacg cgcccccacc cttcgggtag ggggaaaggc cgctaaagcg 4861 gctaaaagcg ctccagcgta tttctgcggg gtttggtgtg gggtttagcg ggctttgccc 4921 gcctttcccc ctgccgcgca gcggtggggc ggtgtgtagc ctagcgcagc gaatagacca @@ -2457,11 +2121,11 @@ ORIGIN 6061 ggatattacc agccacaacg aggattatac cgtggttgag caatatgagc gtagcgaggg 6121 tcgccacagc accggcggca tggacgaact gtataaggga tcctaataa // -LOCUS BASIC_SEVA_56.10 3227 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_5a6.10 3227 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Tetracycline resistance marker and p15A - origin of replication.. -ACCESSION BASIC_SEVA_56 -VERSION BASIC_SEVA_56.10 + origin of replication. +ACCESSION da102cb0396b5d4633d32fa99b1b6cdb +VERSION da102cb0396b5d4633d32fa99b1b6cdb KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -2472,7 +2136,7 @@ FEATURES Location/Qualifiers misc_feature 64..166 /label="SEVA_T0" misc_feature 201..1467 - /label="SEVA_Tet" + /label="SEVA_Tet(5axx)" misc_feature 1480..1532 /label="BSEVA_L1" misc_feature 1541..2272 @@ -2504,7 +2168,7 @@ ORIGIN 781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg - 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc + 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc 1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg 1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt @@ -2543,11 +2207,11 @@ ORIGIN 3121 atattaccag ccacaacgag gattataccg tggttgagca atatgagcgt agcgagggtc 3181 gccacagcac cggcggcatg gacgaactgt ataagggatc ctaataa // -LOCUS BASIC_SEVA_57.10 4151 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_5a7.10 4151 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Tetracycline resistance marker and - pSC101 origin of replication.. -ACCESSION BASIC_SEVA_57 -VERSION BASIC_SEVA_57.10 + pSC101 origin of replication. +ACCESSION d9f4ac855df0c3fcafcab2ae7e872036 +VERSION d9f4ac855df0c3fcafcab2ae7e872036 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -2558,7 +2222,7 @@ FEATURES Location/Qualifiers misc_feature 64..166 /label="SEVA_T0" misc_feature 201..1467 - /label="SEVA_Tet" + /label="SEVA_Tet(5axx)" misc_feature 1480..1532 /label="BSEVA_L1" misc_feature 1541..3000 @@ -2590,7 +2254,7 @@ ORIGIN 781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg - 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc + 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc 1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg 1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt @@ -2645,116 +2309,11 @@ ORIGIN 4081 agcaatatga gcgtagcgag ggtcgccaca gcaccggcgg catggacgaa ctgtataagg 4141 gatcctaata a // -LOCUS BASIC_SEVA_57_TS.10 4220 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_5a9.10 3880 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Tetracycline resistance marker and - temperature sensitive pSC101 origin of replication.. -ACCESSION BASIC_SEVA_57_TS -VERSION BASIC_SEVA_57_TS.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 201..1467 - /label="SEVA_Tet" - misc_feature 1480..1532 - /label="BSEVA_L1" - CDS complement(1594..2544) - /label="Rep101(Ts)" - rep_origin 2592..2814 - /label="pSC101 ori" - misc_feature 3084..3188 - /label="SEVA_T1" - misc_feature 3200..3254 - /label="LMS" - misc_feature 3347..3381 - /label="J23119" - misc_feature 3499..3506 - /label="RBS-A12" - CDS 3513..4217 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttac gtatttaaat ttgacagctt atcatcgata aactgtaatg cggtagttta - 241 tcacagttaa attgctaacg cagtcaggca ccgtgtatga aatctaacaa tgcgctcatc - 301 gtcatcctcg gcaccgtcac cctggatgct gtaggcatag gcttggttat gccggtactg - 361 ccgggcctct tgcgggatat cgtccattcc gacagcatcg ccagtcacta tggcgtgctg - 421 ctagcgctat atgcgttgat gcaatttcta tgcgcacccg ttctcggagc actgtccgac - 481 cgctttggcc gccgcccagt cctgctcgct tcgctacttg gagccactat cgactacgcg - 541 atcatggcga ccacacccgt cctgtggatt ctctacgccg gacgcatcgt ggccggcatc - 601 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat - 661 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc - 721 gtggccgggg gactgttggg cgccatctcc ctgcacgcac cattccttgc ggcggcggtg - 781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag - 841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc - 901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg - 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc - 1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt - 1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg - 1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt - 1201 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta - 1261 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg - 1321 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg - 1381 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt - 1441 gcatggagcc gggccacctc gacctgagac aattgtcggc tcggcccact tgtgtgtctc - 1501 gtacaattct gaggtccaca cagatagtgt cctgttctac ttttgtttgt tagtcttgat - 1561 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat - 1621 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata - 1681 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt - 1741 taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg atgtaatggt - 1801 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc - 1861 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc - 1921 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca - 1981 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc - 2041 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa - 2101 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata - 2161 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc - 2221 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac - 2281 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac - 2341 accataagca ttttccctac tgatgttcat catctgaacg tattggttat aagtgaacga - 2401 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca - 2461 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc - 2521 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata - 2581 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat - 2641 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa - 2701 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat - 2761 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact - 2821 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa - 2881 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcggttg cggccgcaat - 2941 cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt - 3001 tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac - 3061 tacaggcgcg gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt - 3121 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat - 3181 ttgatgcctt taattaaggc tcgggagacc tatcggtaat aacagtccaa tctggtgtaa - 3241 cttcggaatc gtccccaatt attgaacacc cttcggggtg tttttttgtt tctggtctac - 3301 catctcgttg tgataataga cctgaagtgc ctactctgga aaatctttga cagctagctc - 3361 agtcctaggt ataatgctag cagctgtcac cggatgtgct ttccggtctg atgagtccgt - 3421 gaggacgaaa cagcctctac aaataatttt gtttaaggct cgctacgaga agattgttac - 3481 tttcgcagcg ttattatcta aggaggtagt ccatggttag caaaggcgag gcggttatca - 3541 aggagtttat gcgttttaag gttcacatgg agggtagcat gaatggtcac gagttcgaga - 3601 tcgagggtga aggcgagggt cgtccgtacg aaggcaccca gaccgcgaag ctgaaagtga - 3661 ccaagggtgg cccgctgccg ttcagctggg acatcctgag cccgcagttc atgtatggca - 3721 gccgtgcgtt taccaaacac ccggcggaca ttccggatta ctataagcaa agcttcccgg - 3781 aaggttttaa atgggagcgt gttatgaact tcgaagatgg tggcgcggtg accgttaccc - 3841 aggacaccag cctggaggat ggcaccctga tttacaaggt gaaactgcgt ggcaccaact - 3901 ttccgccgga tggtccggtt atgcagaaga aaacgatggg ttgggaagcg agcaccgagc - 3961 gtctgtatcc ggaagatggc gtgctgaagg gtgatatcaa aatggcgctg cgtctgaagg - 4021 acggtggccg ttacctggcg gattttaaga ccacctataa agcgaagaaa ccggtgcaaa - 4081 tgccgggtgc gtacaacgtt gaccgtaaac tggatattac cagccacaac gaggattata - 4141 ccgtggttga gcaatatgag cgtagcgagg gtcgccacag caccggcggc atggacgaac - 4201 tgtataaggg atcctaataa -// -LOCUS BASIC_SEVA_58.10 3424 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Tetracycline resistance marker and pUC - origin of replication.. -ACCESSION BASIC_SEVA_58 -VERSION BASIC_SEVA_58.10 + pBR322-ROP origin of replication. +ACCESSION de15e534a36c66f3ebe7c835782cffa7 +VERSION de15e534a36c66f3ebe7c835782cffa7 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -2765,20 +2324,20 @@ FEATURES Location/Qualifiers misc_feature 64..166 /label="SEVA_T0" misc_feature 201..1467 - /label="SEVA_Tet" + /label="SEVA_Tet(5axx)" misc_feature 1480..1532 /label="BSEVA_L1" - misc_feature 1541..2469 - /label="SEVA_pUC" - misc_feature 2484..2588 + misc_feature 1541..2925 + /label="SEVA_pBR322-ROP" + misc_feature 2940..3044 /label="SEVA_T1" - misc_feature 2600..2654 + misc_feature 3056..3110 /label="LMS" - misc_feature 2655..2689 + misc_feature 3111..3145 /label="J23106" - misc_feature 2698..2709 + misc_feature 3154..3165 /label="B0034" - CDS 2717..3421 + CDS 3173..3877 /label="mScarlet" ORIGIN 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact @@ -2797,7 +2356,7 @@ ORIGIN 781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg - 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc + 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc 1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg 1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt @@ -2809,42 +2368,49 @@ ORIGIN 1501 gtacaattct gaggtccaca cagatagtgt ccggccggcc ccgtagaaaa gatcaaagga 1561 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1621 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact - 1681 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac + 1681 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1741 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1801 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 1861 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga - 1921 acgacctaca ccgaactgag atacctacag cgtgagcttt gagaaagcgc cacgcttccc + 1921 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 1981 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2041 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2101 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2161 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2221 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2281 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc - 2341 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac - 2401 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact - 2461 cattaggcag gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt - 2521 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat - 2581 ttgatgcctt taattaaggc tcgggagacc tatcggtaat aacagtccaa tctggtgtaa - 2641 cttcggaatc gtcctttacg gctagctcag tcctaggtat agtgctagct actagtgaaa - 2701 gaggagaaat actagtatgg ttagcaaagg cgaggcggtt atcaaggagt ttatgcgttt - 2761 taaggttcac atggagggta gcatgaatgg tcacgagttc gagatcgagg gtgaaggcga - 2821 gggtcgtccg tacgaaggca cccagaccgc gaagctgaaa gtgaccaagg gtggcccgct - 2881 gccgttcagc tgggacatcc tgagcccgca gttcatgtat ggcagccgtg cgtttaccaa - 2941 acacccggcg gacattccgg attactataa gcaaagcttc ccggaaggtt ttaaatggga - 3001 gcgtgttatg aacttcgaag atggtggcgc ggtgaccgtt acccaggaca ccagcctgga - 3061 ggatggcacc ctgatttaca aggtgaaact gcgtggcacc aactttccgc cggatggtcc - 3121 ggttatgcag aagaaaacga tgggttggga agcgagcacc gagcgtctgt atccggaaga - 3181 tggcgtgctg aagggtgata tcaaaatggc gctgcgtctg aaggacggtg gccgttacct - 3241 ggcggatttt aagaccacct ataaagcgaa gaaaccggtg caaatgccgg gtgcgtacaa - 3301 cgttgaccgt aaactggata ttaccagcca caacgaggat tataccgtgg ttgagcaata - 3361 tgagcgtagc gagggtcgcc acagcaccgg cggcatggac gaactgtata agggatccta - 3421 ataa + 2341 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact + 2401 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac + 2461 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg + 2521 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt + 2581 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag + 2641 cgtggtcgtg cagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt + 2701 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt + 2761 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac + 2821 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac + 2881 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggggcgc gcccagctgt + 2941 ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg + 3001 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctttaat taaggctcgg + 3061 gagacctatc ggtaataaca gtccaatctg gtgtaacttc ggaatcgtcc tttacggcta + 3121 gctcagtcct aggtatagtg ctagctacta gtgaaagagg agaaatacta gtatggttag + 3181 caaaggcgag gcggttatca aggagtttat gcgttttaag gttcacatgg agggtagcat + 3241 gaatggtcac gagttcgaga tcgagggtga aggcgagggt cgtccgtacg aaggcaccca + 3301 gaccgcgaag ctgaaagtga ccaagggtgg cccgctgccg ttcagctggg acatcctgag + 3361 cccgcagttc atgtatggca gccgtgcgtt taccaaacac ccggcggaca ttccggatta + 3421 ctataagcaa agcttcccgg aaggttttaa atgggagcgt gttatgaact tcgaagatgg + 3481 tggcgcggtg accgttaccc aggacaccag cctggaggat ggcaccctga tttacaaggt + 3541 gaaactgcgt ggcaccaact ttccgccgga tggtccggtt atgcagaaga aaacgatggg + 3601 ttgggaagcg agcaccgagc gtctgtatcc ggaagatggc gtgctgaagg gtgatatcaa + 3661 aatggcgctg cgtctgaagg acggtggccg ttacctggcg gattttaaga ccacctataa + 3721 agcgaagaaa ccggtgcaaa tgccgggtgc gtacaacgtt gaccgtaaac tggatattac + 3781 cagccacaac gaggattata ccgtggttga gcaatatgag cgtagcgagg gtcgccacag + 3841 caccggcggc atggacgaac tgtataaggg atcctaataa // -LOCUS BASIC_SEVA_59.10 3880 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_5a7_pKD46.10 4220 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Tetracycline resistance marker and - pBR322-ROP origin of replication.. -ACCESSION BASIC_SEVA_59 -VERSION BASIC_SEVA_59.10 + temperature sensitive pSC101 origin of replication. +ACCESSION 1319bafa14d98407fe18627921dba1c3 +VERSION 1319bafa14d98407fe18627921dba1c3 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -2855,20 +2421,22 @@ FEATURES Location/Qualifiers misc_feature 64..166 /label="SEVA_T0" misc_feature 201..1467 - /label="SEVA_Tet" + /label="SEVA_Tet(5axx)" misc_feature 1480..1532 /label="BSEVA_L1" - misc_feature 1541..2925 - /label="SEVA_pBR322-ROP" - misc_feature 2940..3044 + CDS 1594..2544 + /label="Rep101(pKD46)" + rep_origin 2592..2814 + /label="pSC101 ori" + misc_feature 3084..3188 /label="SEVA_T1" - misc_feature 3056..3110 + misc_feature 3200..3254 /label="LMS" - misc_feature 3111..3145 + misc_feature 3347..3381 /label="J23119" - misc_feature 3154..3165 - /label="B0034" - CDS 3173..3877 + misc_feature 3499..3506 + /label="RBS-A12" + CDS 3513..4217 /label="mScarlet" ORIGIN 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact @@ -2887,7 +2455,7 @@ ORIGIN 781 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 841 cgccgtccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 901 atgaccattg tggccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg - 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc + 961 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgagc 1021 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 1081 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg 1141 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt @@ -2896,52 +2464,58 @@ ORIGIN 1321 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg 1381 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt 1441 gcatggagcc gggccacctc gacctgagac aattgtcggc tcggcccact tgtgtgtctc - 1501 gtacaattct gaggtccaca cagatagtgt ccggccggcc ccgtagaaaa gatcaaagga - 1561 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg - 1621 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact - 1681 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac - 1741 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg - 1801 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg - 1861 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga - 1921 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc - 1981 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg - 2041 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc - 2101 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc - 2161 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt - 2221 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc - 2281 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc - 2341 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact - 2401 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac - 2461 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg - 2521 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt - 2581 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag - 2641 cgtggtcgtg cagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt - 2701 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt - 2761 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac - 2821 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac - 2881 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggggcgc gcccagctgt - 2941 ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg - 3001 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctttaat taaggctcgg - 3061 gagacctatc ggtaataaca gtccaatctg gtgtaacttc ggaatcgtcc ttgacagcta - 3121 gctcagtcct aggtataatg ctagctacta gtgaaagagg agaaatacta gtatggttag - 3181 caaaggcgag gcggttatca aggagtttat gcgttttaag gttcacatgg agggtagcat - 3241 gaatggtcac gagttcgaga tcgagggtga aggcgagggt cgtccgtacg aaggcaccca - 3301 gaccgcgaag ctgaaagtga ccaagggtgg cccgctgccg ttcagctggg acatcctgag - 3361 cccgcagttc atgtatggca gccgtgcgtt taccaaacac ccggcggaca ttccggatta - 3421 ctataagcaa agcttcccgg aaggttttaa atgggagcgt gttatgaact tcgaagatgg - 3481 tggcgcggtg accgttaccc aggacaccag cctggaggat ggcaccctga tttacaaggt - 3541 gaaactgcgt ggcaccaact ttccgccgga tggtccggtt atgcagaaga aaacgatggg - 3601 ttgggaagcg agcaccgagc gtctgtatcc ggaagatggc gtgctgaagg gtgatatcaa - 3661 aatggcgctg cgtctgaagg acggtggccg ttacctggcg gattttaaga ccacctataa - 3721 agcgaagaaa ccggtgcaaa tgccgggtgc gtacaacgtt gaccgtaaac tggatattac - 3781 cagccacaac gaggattata ccgtggttga gcaatatgag cgtagcgagg gtcgccacag - 3841 caccggcggc atggacgaac tgtataaggg atcctaataa + 1501 gtacaattct gaggtccaca cagatagtgt cctgttctac ttttgtttgt tagtcttgat + 1561 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat + 1621 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcata + 1681 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt + 1741 taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg taggaatctg atgtaatggt + 1801 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc + 1861 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc + 1921 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca + 1981 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc + 2041 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa + 2101 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata + 2161 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc + 2221 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac + 2281 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac + 2341 accataagca ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga + 2401 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca + 2461 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc + 2521 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata + 2581 ccaattgaga tgggctagtc aatgataatt actagtcctt ttcctttgag ttgtgggtat + 2641 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa + 2701 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat + 2761 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact + 2821 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa + 2881 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcggttg cggccgcaat + 2941 cgggcaaatc gctgaatatt ccttttgtct ccgaccatca ggcacctgag tcgctgtctt + 3001 tttcgtgaca ttcagttcgc tgcgctcacg gctctggcag tgaatggggg taaatggcac + 3061 tacaggcgcg gcgcgcccag ctgtctaggg cggcggattt gtcctactca ggagagcgtt + 3121 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat + 3181 ttgatgcctt taattaaggc tcgggagacc tatcggtaat aacagtccaa tctggtgtaa + 3241 cttcggaatc gtccccaatt attgaacacc cttcggggtg tttttttgtt tctggtctac + 3301 catctcgttg tgataataga cctgaagtgc ctactctgga aaatctttga cagctagctc + 3361 agtcctaggt ataatgctag cagctgtcac cggatgtgct ttccggtctg atgagtccgt + 3421 gaggacgaaa cagcctctac aaataatttt gtttaaggct cgctacgaga agattgttac + 3481 tttcgcagcg ttattatcta aggaggtagt ccatggttag caaaggcgag gcggttatca + 3541 aggagtttat gcgttttaag gttcacatgg agggtagcat gaatggtcac gagttcgaga + 3601 tcgagggtga aggcgagggt cgtccgtacg aaggcaccca gaccgcgaag ctgaaagtga + 3661 ccaagggtgg cccgctgccg ttcagctggg acatcctgag cccgcagttc atgtatggca + 3721 gccgtgcgtt taccaaacac ccggcggaca ttccggatta ctataagcaa agcttcccgg + 3781 aaggttttaa atgggagcgt gttatgaact tcgaagatgg tggcgcggtg accgttaccc + 3841 aggacaccag cctggaggat ggcaccctga tttacaaggt gaaactgcgt ggcaccaact + 3901 ttccgccgga tggtccggtt atgcagaaga aaacgatggg ttgggaagcg agcaccgagc + 3961 gtctgtatcc ggaagatggc gtgctgaagg gtgatatcaa aatggcgctg cgtctgaagg + 4021 acggtggccg ttacctggcg gattttaaga ccacctataa agcgaagaaa ccggtgcaaa + 4081 tgccgggtgc gtacaacgtt gaccgtaaac tggatattac cagccacaac gaggattata + 4141 ccgtggttga gcaatatgag cgtagcgagg gtcgccacag caccggcggc atggacgaac + 4201 tgtataaggg atcctaataa // -LOCUS BASIC_SEVA_65.10 5699 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_65a.10 5699 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Gentamicin resistance marker and - RSF1010 origin of replication.. -ACCESSION BASIC_SEVA_65 -VERSION BASIC_SEVA_65.10 + RSF1010 origin of replication. +ACCESSION ba9809f0bba102f479d902c3fb82b5f7 +VERSION ba9809f0bba102f479d902c3fb82b5f7 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -2955,8 +2529,8 @@ FEATURES Location/Qualifiers /label="SEVA_Gm" misc_feature 1010..1062 /label="BSEVA_L1" - misc_feature 1071..4744 - /label="SEVA_RSF101" + misc_feature 1074..4744 + /label="SEVA_RSF1010(x5ax)" misc_feature 4759..4863 /label="SEVA_T1" misc_feature 4875..4929 @@ -2985,61 +2559,61 @@ ORIGIN 841 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga 901 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga 961 gatcggcttc ccggccgcgg agttgttcgg taaattggac aacggtcggc tcggcccact - 1021 tgtgtgtctc gtacaattct gaggtccaca cagatagtgt ccggccggcc tcagcctgcc - 1081 gccttgggcc gggtgatgtc gtacttgccc gccgcgaact cggttaccgt ccagcccagc - 1141 gcgaccagct ccggcaacgc ctcgcgcacc cgctggcggc gcttgcgcat ggtcgaacca - 1201 ctggcctctg acggccagac atagccgcac aaggtatcta tggaagcctt gccggttttg - 1261 ccggggtcga tccagccaca cagccgctgg tgcagcaggc gggcggtttc gctgtccagc - 1321 gcccgcacct cgtccatgct gatgcgcaca tgctggccgc cacccatgac ggcctgcgcg - 1381 atcaaggggt tcagggccac gtacaggcgc ccgtccgcct cgtcgctggc gtactccgac - 1441 agcagccgaa acccctgccg cttgcggcca ttctgggcga tgatggatac cttccaaagg - 1501 cgctcgatgc agtcctgtat gtgcttgagc gccccaccac tatcgacctc tgccccgatt - 1561 tcctttgcca gcgcccgata gctacctttg accacatggc attcagcggt gacggcctcc - 1621 cacttgggtt ccaggaacag ccggagctgc cgtccgcctt cggtcttggg ttccgggcca - 1681 agcactaggc cattaggccc agccatggcc accagccctt gcaggatgcg cagatcatca - 1741 gcgcccagcg gctccgggcc gctgaactcg atccgcttgc cgtcgccgta gtcatacgtc - 1801 acgtccagct tgctgcgctt gcgctcgccc cgcttgaggg cacggaacag gccgggggcc - 1861 agacagtgcg ccgggtcgtg ccggacgtgg ctgaggctgt gcttgttctt aggcttcacc - 1921 acggggcacc cccttgctct tgcgctgcct ctccagcacg gcgggcttga gcaccccgcc - 1981 gtcatgccgc ctgaaccacc gatcagcgaa cggtgcgcca tagttggcct tgctcacacc - 2041 gaagcggacg aagaaccggc gctggtcgtc gtccacaccc cattcctcgg cctcggcgct - 2101 ggtcatgctc gacaggtagg actgccagcg gatgttatcg accagtaccg agctgccccg - 2161 gctggcctgc tgctggtcgc ctgcgcccat catggccgcg cccttgctgg catggtgcag - 2221 gaacacgata gagcacccgg tatcggcggc gatggcctcc atgcgaccga tgacctgggc - 2281 catggggccg ctggcgtttt cttcctcgat gtggaaccgg cgcagcgtgt ccagcaccat - 2341 caggcggcgg ccctcggcgg cgcgcttgag gccgtcgaac cactccgggg ccatgatgtt - 2401 gggcaggctg ccgatcagcg gctggatcag caggccgtca gccacggctt gccgttcctc - 2461 ggcgctgagg tgcgccccaa gggcgtgcag gcggtgatga atggcggtgg gcgggtcttc - 2521 ggcgggcagg tagatcaccg ggccggtggg cagttcgccc acctccagca gatccggccc - 2581 gcctgcaatc tgtgcggcca gttgcagggc cagcatggat ttaccggcac caccgggcga - 2641 caccagcgcc ccgaccgtac cggccaccat gttgggcaaa acgtagtcca gcggtggcgg - 2701 cgctgctgcg aacgcctcca gaatattgat aggcttatgg gtagccattg attgcctcct - 2761 ttgcaggcag ttggtggtta ggcgctggcg gggtcactac ccccgccctg cgccgctctg - 2821 agttcttcca ggcactcgcg cagcgcctcg tattcgtcgt cggtcagcca gaacttgcgc - 2881 tgacgcatcc ctttggcctt catgcgctcg gcatatcgcg cttggcgtac agcgtcaggg - 2941 ctggccagca ggtcgccggt ctgcttgtcc ttttggtctt tcatatcagt caccgagaaa - 3001 cttgccgggg ccgaaaggct tgtcttcgcg gaacaaggac aaggtgcagc cgtcaaggtt - 3061 aaggctggcc atatcagcga ctgaaaagcg gccagcctcg gccttgtttg acgtataacc - 3121 aaagccaccg ggcaaccaat agcccttgtc acttttgatc aggtagaccg accctgaagc - 3181 gcttttttcg tattccataa aacccccttc tgtgcgtgag tactcatagt ataacaggcg - 3241 tgagtaccaa cgcaagcact acatgctgaa atctggcccg cccctgtcca tgcctcgctg - 3301 gcggggtgcc ggtgcccgtg ccagctcggc ccgcgcaagc tggacgctgg gcagacccat - 3361 gaccttgctg acggtgcgct cgatgtaatc cgcttcgtgg ccgggcttgc gctctgccag - 3421 cgctgggctg gcctcggcca tggccttgcc gatttcctcg gcactgcggc cccggctggc - 3481 cagcttctgc gcggcgataa agtcgcactt gctgaggtca tcaccgaagc gcttgaccag - 3541 cccggccatc tcgctgcggt actcgtccag cgccgtgcgc cggtggcggc taagctgccg - 3601 ctcgggcagt tcgaggctgg ccagcctgcg ggccttctcc tgctgccgct gggcctgctc - 3661 gatctgctgg ccagcctgct gcaccagcgc cgggccagcg gtggcggtct tgcccttgga - 3721 ttcacgcagc agcacccacg gctgataacc ggcgcgggtg gtgtgcttgt ccttgcggtt - 3781 ggtgaagccc gccaagcggc catagtggcg gctgtcggcg ctggccgggt cggcgtcgta - 3841 ctcgctggcc agcgtccggg caatctgccc ccgaagttca ccgcctgcgg cgtcggccac - 3901 cttgacccat gcctgatagt tcttcgggct ggtttccact accagggcag gctcccggcc - 3961 ctcggctttc atgtcatcca ggtcaaactc gctgaggtcg tccaccagca ccagaccatg - 4021 ccgctcctgc tcggcgggcc tgatatacac gtcattgccc tgggcattca tccgcttgag - 4081 ccatggcgtg ttctggagca cttcggcggc tgaccattcc cggttcatca tctggccggt - 4141 ggtggcgtcc ctgacgccga tatcgaagcg ctcacagccc atggccttga gctgtcggcc - 4201 tatggcctgc aaagtcctgt cgttcttcat cgggccacca agcgattccc acacattata - 4261 cgagccggaa gcataaagtg taaagcctag atccgaagga tgagccgggc tgaatgatcg + 1021 tgtgtgtctc gtacaattct gaggtccaca cagatagtgt ccgtaggccg gcctcagcct + 1081 gccgccttgg gccgggtgat gtcgtacttg cccgccgcga actcggttac cgtccagccc + 1141 agcgcgacca gctccggcaa cgcctcgcgc acccgctggc ggcgcttgcg catggtcgaa + 1201 ccactggcct ctgacggcca gacatagccg cacaaggtat ctatggaagc cttgccggtt + 1261 ttgccggggt cgatccagcc acacagccgc tggtgcagca ggcgggcggt ttcgctgtcc + 1321 agcgcccgca cctcgtccat gctgatgcgc acatgctggc cgccacccat gacggcctgc + 1381 gcgatcaagg ggttcagggc cacgtacagg cgcccgtccg cctcgtcgct ggcgtactcc + 1441 gacagcagcc gaaacccctg ccgcttgcgg ccattctggg cgatgatgga taccttccaa + 1501 aggcgctcga tgcagtcctg tatgtgcttg agcgccccac cactatcgac ctctgccccg + 1561 atttcctttg ccagcgcccg atagctacct ttgaccacca tggcatcagc ggtgacggcc + 1621 tcccacttgg gttccaggaa cagccggagc tgccgtccgc cttcggtctt gggttccggg + 1681 ccaagcacta ggccattagg cccagccatg gccaccagcc cttgcaggat gcgcagatca + 1741 tcagcgccca gcggctccgg gccgctgaac tcgatccgct tgccgtcgcc gtagtcatac + 1801 gtcacgtcca gcttgctgcg cttgcgctcg ccccgcttga gggcacggaa caggccgggg + 1861 gccagacagt gcgccgggtc gtgccggacg tggctgaggc tgtgcttgtt cttaggcttc + 1921 accacggggc acccccttgc tcttgcgctg cctctccagc acggcgggct tgagcacccc + 1981 gccgtcatgc cgcctgaacc accgatcagc gaacggtgcg ccatagttgg ccttgctcac + 2041 accgaagcgg acgaagaacc ggcgctggtc gtcgtccaca ccccattcct cggcctcggc + 2101 gctggtcatg ctcgacaggt aggactgcca gcggatgtta tcgaccagta ccgagctgcc + 2161 ccggctggcc tgctgctggt cgcctgcgcc catcatggcc gcgcccttgc tggcatggtg + 2221 caggaacacg atagagcacc cggtatcggc ggcgatggcc tccatgcgac cgatgacctg + 2281 ggccatgggg ccgctggcgt tttcttcctc gatgtggaac cggcgcagcg tgtccagcac + 2341 catcaggcgg cggccctcgg cggcgcgctt gaggccgtcg aaccactccg gggccatgat + 2401 gttgggcagg ctgccgatca gcggctggat cagcaggccg tcagccacgg cttgccgttc + 2461 ctcggcgctg aggtgcgccc caagggcgtg caggcggtga tgaatggcgg tgggcgggtc + 2521 ttcggcgggc aggtagatca ccgggccggt gggcagttcg cccacctcca gcagatccgg + 2581 cccgcctgca atctgtgcgg ccagttgcag ggccagcatg gatttaccgg caccaccggg + 2641 cgacaccagc gccccgaccg taccggccac catgttgggc aaaacgtagt ccagcggtgg + 2701 cggcgctgct gcgaacgcct ccagaatatt gataggctta tgggtagcca ttgattgcct + 2761 cctttgcagg cagttggtgg ttaggcgctg gcggggtcac tacccccgcc ctgcgccgct + 2821 ctgagttctt ccaggcactc gcgcagcgcc tcgtattcgt cgtcggtcag ccagaacttg + 2881 cgctgacgca tccctttggc cttcatgcgc tcggcatatc gcgcttggcg tacagcgtca + 2941 gggctggcca gcaggtcgcc ggtctgcttg tccttttggt ctttcatatc agtcaccgag + 3001 aaacttgccg gggccgaaag gcttgtcttc gcggaacaag gacaaggtgc agccgtcaag + 3061 gttaaggctg gccatatcag cgactgaaaa gcggccagcc tcggccttgt ttgacgtata + 3121 accaaagcca ccgggcaacc aatagccctt gtcacttttg atcaggtaga ccgaccctga + 3181 agcgcttttt tcgtattcca taaaaccccc ttctgtgcgt gagtactcat agtataacag + 3241 gcgtgagtac caacgcaagc actacatgct gaaatctggc ccgcccctgt ccatgcctcg + 3301 ctggcggggt gccggtgccc gtgccagctc ggcccgcgca agctggacgc tgggcagacc + 3361 catgaccttg ctgacggtgc gctcgatgta atccgcttcg tggccgggct tgcgctctgc + 3421 cagcgctggg ctggcctcgg ccatggcctt gccgatttcc tcggcactgc ggccccggct + 3481 ggccagcttc tgcgcggcga taaagtcgca cttgctgagg tcatcaccga agcgcttgac + 3541 cagcccggcc atctcgctgc ggtactcgtc cagcgccgtg cgccggtggc ggctaagctg + 3601 ccgctcgggc agttcgaggc tggccagcct gcgggccttc tcctgctgcc gctgggcctg + 3661 ctcgatctgc tggccagcct gctgcaccag cgccgggcca gcggtggcgg tcttgccctt + 3721 ggattcacgc agcagcaccc acggctgata accggcgcgg gtggtgtgct tgtccttgcg + 3781 gttggtgaag cccgccaagc ggccatagtg gcggctgtcg gcgctggccg ggtcggcgtc + 3841 gtactcgctg gccagcgtcc gggcaatctg cccccgaagt tcaccgcctg cggcgtcggc + 3901 caccttgacc catgcctgat agttcttcgg gctggtttcc actaccaggg caggctcccg + 3961 gccctcggct ttcatgtcat ccaggtcaaa ctcgctgagg tcgtccacca gcaccagacc + 4021 atgccgctcc tgctcggcgg gcctgatata cacgtcattg ccctgggcat tcatccgctt + 4081 gagccatggc gtgttctgga gcacttcggc ggctgaccat tcccggttca tcatctggcc + 4141 ggtggtggcg tccctgacgc cgatatcgaa gcgctcacag cccatggcct tgagctgtcg + 4201 gcctatggcc tgcaaagtcc tgtcgttctt catcgggcca ccaagcgatt cccacacatt + 4261 atacgagccg gaagcataaa gtgtagctag atccgaagga tgagccgggc tgaatgatcg 4321 accgagacag gccctgcggg gctgcacacg cgcccccacc cttcgggtag ggggaaaggc 4381 cgctaaagcg gctaaaagcg ctccagcgta tttctgcggg gtttggtgtg gggtttagcg 4441 ggctttgccc gcctttcccc ctgccgcgca gcggtggggc ggtgtgtagc ctagcgcagc @@ -3064,11 +2638,11 @@ ORIGIN 5581 accgtaaact ggatattacc agccacaacg aggattatac cgtggttgag caatatgagc 5641 gtagcgaggg tcgccacagc accggcggca tggacgaact gtataaggga tcctaataa // -LOCUS BASIC_SEVA_66.10 2757 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_66.10 2757 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Gentamicin resistance marker and p15A - origin of replication.. -ACCESSION BASIC_SEVA_66 -VERSION BASIC_SEVA_66.10 + origin of replication. +ACCESSION 9874d4b94b5ebed8ea99f69534b4656e +VERSION 9874d4b94b5ebed8ea99f69534b4656e KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -3142,11 +2716,11 @@ ORIGIN 2641 cgtaaactgg atattaccag ccacaacgag gattataccg tggttgagca atatgagcgt 2701 agcgagggtc gccacagcac cggcggcatg gacgaactgt ataagggatc ctaataa // -LOCUS BASIC_SEVA_67.10 3681 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_67.10 3681 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Gentamicin resistance marker and pSC101 - origin of replication.. -ACCESSION BASIC_SEVA_67 -VERSION BASIC_SEVA_67.10 + origin of replication. +ACCESSION db12df1f8d044fdd49a6237418780dd1 +VERSION db12df1f8d044fdd49a6237418780dd1 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -3236,11 +2810,100 @@ ORIGIN 3601 accgtggttg agcaatatga gcgtagcgag ggtcgccaca gcaccggcgg catggacgaa 3661 ctgtataagg gatcctaata a // -LOCUS BASIC_SEVA_67_TS.10 3750 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_SEVA_69.10 3410 bp DNA circular UNK 03-NOV-2021 +DEFINITION BASIC SEVA vector containing Gentamicin resistance marker and + pBR322-ROP origin of replication. +ACCESSION 7cce40252f64427685493882230c8bf1 +VERSION 7cce40252f64427685493882230c8bf1 +KEYWORDS . +SOURCE synthetic construct + ORGANISM synthetic construct + other sequences; artificial sequences. +FEATURES Location/Qualifiers + misc_feature 3..57 + /label="LMP" + misc_feature 64..166 + /label="SEVA_T0" + misc_feature 193..997 + /label="SEVA_Gm" + misc_feature 1010..1062 + /label="BSEVA_L1" + misc_feature 1071..2455 + /label="SEVA_pBR322-ROP" + misc_feature 2470..2574 + /label="SEVA_T1" + misc_feature 2586..2640 + /label="LMS" + misc_feature 2641..2675 + /label="J23106" + misc_feature 2684..2695 + /label="B0034" + CDS 2703..3407 + /label="mScarlet" +ORIGIN + 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact + 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc + 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat + 181 tacgatttaa atttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg + 241 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg + 301 gttttcatgg cttgttatga ctgttttttt gtacagccta tgcctcgggc atccaagcag + 361 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag + 421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat + 481 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct + 541 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc + 601 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca + 661 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag + 721 tgagatctat atctatgatc tcgcagtctc cggagagcac cggaggcagg gcattgccac + 781 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt + 841 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga + 901 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga + 961 gatcggcttc ccggccgcgg agttgttcgg taaattggac aacggtcggc tcggcccact + 1021 tgtgtgtctc gtacaattct gaggtccaca cagatagtgt ccggccggcc ccgtagaaaa + 1081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa + 1141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc + 1201 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta + 1261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct + 1321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg + 1381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag + 1441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc + 1501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg + 1561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt + 1621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg + 1681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca + 1741 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg + 1801 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc + 1861 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat + 1921 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg + 1981 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg + 2041 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg + 2101 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa + 2161 agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc cgcgtccagc + 2221 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg + 2281 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg + 2341 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat + 2401 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggggcgc + 2461 gcccagctgt ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac + 2521 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctttaat + 2581 taaggctcgg gagacctatc ggtaataaca gtccaatctg gtgtaacttc ggaatcgtcc + 2641 tttacggcta gctcagtcct aggtatagtg ctagctacta gtgaaagagg agaaatacta + 2701 gtatggttag caaaggcgag gcggttatca aggagtttat gcgttttaag gttcacatgg + 2761 agggtagcat gaatggtcac gagttcgaga tcgagggtga aggcgagggt cgtccgtacg + 2821 aaggcaccca gaccgcgaag ctgaaagtga ccaagggtgg cccgctgccg ttcagctggg + 2881 acatcctgag cccgcagttc atgtatggca gccgtgcgtt taccaaacac ccggcggaca + 2941 ttccggatta ctataagcaa agcttcccgg aaggttttaa atgggagcgt gttatgaact + 3001 tcgaagatgg tggcgcggtg accgttaccc aggacaccag cctggaggat ggcaccctga + 3061 tttacaaggt gaaactgcgt ggcaccaact ttccgccgga tggtccggtt atgcagaaga + 3121 aaacgatggg ttgggaagcg agcaccgagc gtctgtatcc ggaagatggc gtgctgaagg + 3181 gtgatatcaa aatggcgctg cgtctgaagg acggtggccg ttacctggcg gattttaaga + 3241 ccacctataa agcgaagaaa ccggtgcaaa tgccgggtgc gtacaacgtt gaccgtaaac + 3301 tggatattac cagccacaac gaggattata ccgtggttga gcaatatgag cgtagcgagg + 3361 gtcgccacag caccggcggc atggacgaac tgtataaggg atcctaataa +// +LOCUS BASIC_SEVA_67_pKD46.10 3750 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC SEVA vector containing Gentamicin resistance marker and - temperature sensitive pSC101 origin of replication.. -ACCESSION BASIC_SEVA_67_TS -VERSION BASIC_SEVA_67_TS.10 + temperature sensitive pSC101 origin of replication. +ACCESSION 4fe6ecc619de0d790bdb44aeef93df39 +VERSION 4fe6ecc619de0d790bdb44aeef93df39 KEYWORDS . SOURCE synthetic construct ORGANISM synthetic construct @@ -3254,8 +2917,8 @@ FEATURES Location/Qualifiers /label="SEVA_Gm" misc_feature 1010..1062 /label="BSEVA_L1" - CDS complement(1124..2074) - /label="Rep101(Ts)" + CDS 1124..2074 + /label="Rep101(pKD46)" rep_origin 2122..2344 /label="pSC101 ori" misc_feature 2614..2718 @@ -3290,7 +2953,7 @@ ORIGIN 1081 tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt 1141 tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat 1201 tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa - 1261 tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg taggaatctg + 1261 tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg taggaatctg 1321 atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg 1381 ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc 1441 ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt @@ -3300,7 +2963,7 @@ ORIGIN 1681 ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa 1741 aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa 1801 agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt - 1861 tagctaatac accataagca ttttccctac tgatgttcat catctgaacg tattggttat + 1861 tagctaatac accataagca ttttccctac tgatgttcat catctgagcg tattggttat 1921 aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg 1981 ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta 2041 gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt @@ -3333,174 +2996,3 @@ ORIGIN 3661 gaggattata ccgtggttga gcaatatgag cgtagcgagg gtcgccacag caccggcggc 3721 atggacgaac tgtataaggg atcctaataa // -LOCUS BASIC_SEVA_68.10 2954 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Gentamicin resistance marker and pUC - origin of replication.. -ACCESSION BASIC_SEVA_68 -VERSION BASIC_SEVA_68.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 193..997 - /label="SEVA_Gm" - misc_feature 1010..1062 - /label="BSEVA_L1" - misc_feature 1071..1999 - /label="SEVA_pUC" - misc_feature 2014..2118 - /label="SEVA_T1" - misc_feature 2130..2184 - /label="LMS" - misc_feature 2185..2219 - /label="J23106" - misc_feature 2228..2239 - /label="B0034" - CDS 2247..2951 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttaa atttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg - 241 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg - 301 gttttcatgg cttgttatga ctgttttttt gtacagccta tgcctcgggc atccaagcag - 361 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag - 421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat - 481 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct - 541 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc - 601 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca - 661 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag - 721 tgagatctat atctatgatc tcgcagtctc cggagagcac cggaggcagg gcattgccac - 781 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt - 841 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga - 901 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga - 961 gatcggcttc ccggccgcgg agttgttcgg taaattggac aacggtcggc tcggcccact - 1021 tgtgtgtctc gtacaattct gaggtccaca cagatagtgt ccggccggcc ccgtagaaaa - 1081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa - 1141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc - 1201 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta - 1261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct - 1321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg - 1381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag - 1441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagcttt gagaaagcgc - 1501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg - 1561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt - 1621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg - 1681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca - 1741 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg - 1801 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc - 1861 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag - 1921 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag - 1981 ttagctcact cattaggcag gcgcgcccag ctgtctaggg cggcggattt gtcctactca - 2041 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct - 2101 ttcgttttat ttgatgcctt taattaaggc tcgggagacc tatcggtaat aacagtccaa - 2161 tctggtgtaa cttcggaatc gtcctttacg gctagctcag tcctaggtat agtgctagct - 2221 actagtgaaa gaggagaaat actagtatgg ttagcaaagg cgaggcggtt atcaaggagt - 2281 ttatgcgttt taaggttcac atggagggta gcatgaatgg tcacgagttc gagatcgagg - 2341 gtgaaggcga gggtcgtccg tacgaaggca cccagaccgc gaagctgaaa gtgaccaagg - 2401 gtggcccgct gccgttcagc tgggacatcc tgagcccgca gttcatgtat ggcagccgtg - 2461 cgtttaccaa acacccggcg gacattccgg attactataa gcaaagcttc ccggaaggtt - 2521 ttaaatggga gcgtgttatg aacttcgaag atggtggcgc ggtgaccgtt acccaggaca - 2581 ccagcctgga ggatggcacc ctgatttaca aggtgaaact gcgtggcacc aactttccgc - 2641 cggatggtcc ggttatgcag aagaaaacga tgggttggga agcgagcacc gagcgtctgt - 2701 atccggaaga tggcgtgctg aagggtgata tcaaaatggc gctgcgtctg aaggacggtg - 2761 gccgttacct ggcggatttt aagaccacct ataaagcgaa gaaaccggtg caaatgccgg - 2821 gtgcgtacaa cgttgaccgt aaactggata ttaccagcca caacgaggat tataccgtgg - 2881 ttgagcaata tgagcgtagc gagggtcgcc acagcaccgg cggcatggac gaactgtata - 2941 agggatccta ataa -// -LOCUS BASIC_SEVA_69.10 3410 bp DNA circular UNK 09-SEP-2021 -DEFINITION BASIC SEVA vector containing Gentamicin resistance marker and - pBR322-ROP origin of replication.. -ACCESSION BASIC_SEVA_69 -VERSION BASIC_SEVA_69.10 -KEYWORDS . -SOURCE synthetic construct - ORGANISM synthetic construct - other sequences; artificial sequences. -FEATURES Location/Qualifiers - misc_feature 3..57 - /label="LMP" - misc_feature 64..166 - /label="SEVA_T0" - misc_feature 193..997 - /label="SEVA_Gm" - misc_feature 1010..1062 - /label="BSEVA_L1" - misc_feature 1071..2455 - /label="SEVA_pBR322-ROP" - misc_feature 2470..2574 - /label="SEVA_T1" - misc_feature 2586..2640 - /label="LMS" - misc_feature 2641..2675 - /label="J23119" - misc_feature 2684..2695 - /label="B0034" - CDS 2703..3407 - /label="mScarlet" -ORIGIN - 1 ggctcgggta agaactcgca cttcgtggaa acactattat ctggtgggtc tctgtccact - 61 agtcttggac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc - 121 agaacgctcg gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat - 181 tacgatttaa atttgacata agcctgttcg gttcgtaaac tgtaatgcaa gtagcgtatg - 241 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg - 301 gttttcatgg cttgttatga ctgttttttt gtacagccta tgcctcgggc atccaagcag - 361 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag - 421 cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg gctcaagtat - 481 gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc gggctgctct - 541 tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc agccggactc - 601 cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg ccttcgacca - 661 agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc agccgcgtag - 721 tgagatctat atctatgatc tcgcagtctc cggagagcac cggaggcagg gcattgccac - 781 cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg tgatctacgt - 841 gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg gcatacggga - 901 agaagtgatg cactttgata tcgacccaag taccgccacc taacaattcg ttcaagccga - 961 gatcggcttc ccggccgcgg agttgttcgg taaattggac aacggtcggc tcggcccact - 1021 tgtgtgtctc gtacaattct gaggtccaca cagatagtgt ccggccggcc ccgtagaaaa - 1081 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa - 1141 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc - 1201 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta - 1261 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct - 1321 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg - 1381 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag - 1441 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc - 1501 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg - 1561 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt - 1621 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg - 1681 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca - 1741 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg - 1801 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc - 1861 ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat - 1921 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg - 1981 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg - 2041 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg - 2101 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa - 2161 agctcatcag cgtggtcgtg cagcgattca cagatgtctg cctgttcatc cgcgtccagc - 2221 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg - 2281 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg - 2341 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat - 2401 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggggcgc - 2461 gcccagctgt ctagggcggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac - 2521 agataaaacg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctttaat - 2581 taaggctcgg gagacctatc ggtaataaca gtccaatctg gtgtaacttc ggaatcgtcc - 2641 ttgacagcta gctcagtcct aggtataatg ctagctacta gtgaaagagg agaaatacta - 2701 gtatggttag caaaggcgag gcggttatca aggagtttat gcgttttaag gttcacatgg - 2761 agggtagcat gaatggtcac gagttcgaga tcgagggtga aggcgagggt cgtccgtacg - 2821 aaggcaccca gaccgcgaag ctgaaagtga ccaagggtgg cccgctgccg ttcagctggg - 2881 acatcctgag cccgcagttc atgtatggca gccgtgcgtt taccaaacac ccggcggaca - 2941 ttccggatta ctataagcaa agcttcccgg aaggttttaa atgggagcgt gttatgaact - 3001 tcgaagatgg tggcgcggtg accgttaccc aggacaccag cctggaggat ggcaccctga - 3061 tttacaaggt gaaactgcgt ggcaccaact ttccgccgga tggtccggtt atgcagaaga - 3121 aaacgatggg ttgggaagcg agcaccgagc gtctgtatcc ggaagatggc gtgctgaagg - 3181 gtgatatcaa aatggcgctg cgtctgaagg acggtggccg ttacctggcg gattttaaga - 3241 ccacctataa agcgaagaaa ccggtgcaaa tgccgggtgc gtacaacgtt gaccgtaaac - 3301 tggatattac cagccacaac gaggattata ccgtggttga gcaatatgag cgtagcgagg - 3361 gtcgccacag caccggcggc atggacgaac tgtataaggg atcctaataa -// diff --git a/basicsynbio/utils/seva.py b/basicsynbio/utils/seva.py index b9c9503..2a764ce 100644 --- a/basicsynbio/utils/seva.py +++ b/basicsynbio/utils/seva.py @@ -8,7 +8,7 @@ "1": "Ampicillin", "2": "Kanamycin", "3": "Chloramphenicol", - "4": "Streptomycin", + "4": "Streptomycin/Spectinomycin", "5": "Tetracycline", "6": "Gentamicin", } diff --git a/csv_xlsx_files/AbR_marker_clip_masses.csv b/csv_xlsx_files/AbR_marker_clip_masses.csv new file mode 100644 index 0000000..1398c95 --- /dev/null +++ b/csv_xlsx_files/AbR_marker_clip_masses.csv @@ -0,0 +1,7 @@ +Marker ID,mass (ng) +BS_1xx,191 +BS_2xx,186 +BS_3xx,179 +BS_4xx,189 +BS_5axx,202 +BS_6xx,180 diff --git a/csv_xlsx_files/BASIC_SEVA_10_abbreviations.csv b/csv_xlsx_files/BASIC_SEVA_10_abbreviations.csv new file mode 100644 index 0000000..1b7dd95 --- /dev/null +++ b/csv_xlsx_files/BASIC_SEVA_10_abbreviations.csv @@ -0,0 +1,31 @@ +Abbreviations +15a +16 +17 +19 +17_pKD46 +25a +26 +27 +29 +27_pKD46 +35a +36 +37 +39 +37_pKD46 +45a +46 +47 +49 +47_pKD46 +5a5a +5a6 +5a7 +5a9 +5a7_pKD46 +65a +66 +67 +69 +67_pKD46 diff --git a/csv_xlsx_files/BASIC_SEVA_10_addgene_batch_upload.csv b/csv_xlsx_files/BASIC_SEVA_10_addgene_batch_upload.csv new file mode 100644 index 0000000..95f7b58 --- /dev/null +++ b/csv_xlsx_files/BASIC_SEVA_10_addgene_batch_upload.csv @@ -0,0 +1,31 @@ +Plasmid Name,Plasmid Type,Purpose,PubMed ID,Gene or insert Name,Alternative Gene/Insert Name 1,Alternative Gene/Insert Name 2,Entrez Gene ID,gRNA/shRNA Sequence,Insert Size,Species of gene or insert,Additional Species of gene or insert,Species Other,Relevant Mutations,N-terminal Tag,C-terminal Tag,Backbone Name,Backbone Manufacturer,Primary Vector Type,Additional Vector Type 1,Additional Vector Type 2,Vector Type Other,Backbone size without insert,Cloning Method,Cloning Site 5-prime,5-prime Site Destroyed in Cloning,Cloning Site 3-prime,3-prime Site Destroyed in Cloning,Promoter,5-prime Sequencing Primer,3-prime Sequencing Primer,Bacterial Resistance,Bacterial Resistance Other,Selectable Marker,Selectable Marker Other,high or low copy,Growth Temp,Growth Strain,Growth Strain Other,Hazardous,Patents or Licenses,Patent/License Explanation,Gene Source,Comments,Sequence:Full,Sequence:partial 1 description,Sequence:partial 1,Sequence:partial 2 description,Sequence:partial 2 +BASIC_SEVA_15a.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_15a.10,,Synthetic Biology,,,,5055,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Ampicillin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTAGTAGCCCGCCTAATGAGCGGGCTTTTTTTTAATTCCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCATTCAGCATTTTCGTGTGGCGCTGATTCCGTTTTTTGCGGCGTTTTGCCTGCCGGTGTTTGCGCATCCGGAAACCCTGGTGAAAGTGAAAGATGCGGAAGATCAACTGGGTGCGCGCGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTGGAATCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGCGGTGCGGTTCTGAGCCGTGTGGATGCGGGCCAGGAACAACTGGGCCGTCGTATTCATTATAGCCAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATGACCGTGCGTGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTGCTGCTGACGACCATTGGCGGTCCGAAAGAACTGACCGCGTTTCTGCATAACATGGGCGATCATGTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAACGTGATACCACCATGCCGGCAGCAATGGCGACCACCCTGCGTAAACTGCTGACGGGTGAGCTGCTGACCCTGGCAAGCCGCCAGCAACTGATTGATTGGATGGAAGCGGATAAAGTGGCGGGTCCGCTGCTGCGTAGCGCGCTGCCGGCTGGCTGGTTTATTGCGGATAAAAGCGGTGCGGGCGAACGTGGCAGCCGTGGCATTATTGCGGCGCTGGGCCCGGATGGTAAACCGAGCCGTATTGTGGTGATTTATACCACCGGCAGCCAGGCGACGATGGATGAACGTAACCGTCAGATTGCGGAAATTGGCGCGAGCCTGATTAAACATTGGTAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGACGACCCTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGTAGGCCGGCCTCAGCCTGCCGCCTTGGGCCGGGTGATGTCGTACTTGCCCGCCGCGAACTCGGTTACCGTCCAGCCCAGCGCGACCAGCTCCGGCAACGCCTCGCGCACCCGCTGGCGGCGCTTGCGCATGGTCGAACCACTGGCCTCTGACGGCCAGACATAGCCGCACAAGGTATCTATGGAAGCCTTGCCGGTTTTGCCGGGGTCGATCCAGCCACACAGCCGCTGGTGCAGCAGGCGGGCGGTTTCGCTGTCCAGCGCCCGCACCTCGTCCATGCTGATGCGCACATGCTGGCCGCCACCCATGACGGCCTGCGCGATCAAGGGGTTCAGGGCCACGTACAGGCGCCCGTCCGCCTCGTCGCTGGCGTACTCCGACAGCAGCCGAAACCCCTGCCGCTTGCGGCCATTCTGGGCGATGATGGATACCTTCCAAAGGCGCTCGATGCAGTCCTGTATGTGCTTGAGCGCCCCACCACTATCGACCTCTGCCCCGATTTCCTTTGCCAGCGCCCGATAGCTACCTTTGACCACCATGGCATCAGCGGTGACGGCCTCCCACTTGGGTTCCAGGAACAGCCGGAGCTGCCGTCCGCCTTCGGTCTTGGGTTCCGGGCCAAGCACTAGGCCATTAGGCCCAGCCATGGCCACCAGCCCTTGCAGGATGCGCAGATCATCAGCGCCCAGCGGCTCCGGGCCGCTGAACTCGATCCGCTTGCCGTCGCCGTAGTCATACGTCACGTCCAGCTTGCTGCGCTTGCGCTCGCCCCGCTTGAGGGCACGGAACAGGCCGGGGGCCAGACAGTGCGCCGGGTCGTGCCGGACGTGGCTGAGGCTGTGCTTGTTCTTAGGCTTCACCACGGGGCACCCCCTTGCTCTTGCGCTGCCTCTCCAGCACGGCGGGCTTGAGCACCCCGCCGTCATGCCGCCTGAACCACCGATCAGCGAACGGTGCGCCATAGTTGGCCTTGCTCACACCGAAGCGGACGAAGAACCGGCGCTGGTCGTCGTCCACACCCCATTCCTCGGCCTCGGCGCTGGTCATGCTCGACAGGTAGGACTGCCAGCGGATGTTATCGACCAGTACCGAGCTGCCCCGGCTGGCCTGCTGCTGGTCGCCTGCGCCCATCATGGCCGCGCCCTTGCTGGCATGGTGCAGGAACACGATAGAGCACCCGGTATCGGCGGCGATGGCCTCCATGCGACCGATGACCTGGGCCATGGGGCCGCTGGCGTTTTCTTCCTCGATGTGGAACCGGCGCAGCGTGTCCAGCACCATCAGGCGGCGGCCCTCGGCGGCGCGCTTGAGGCCGTCGAACCACTCCGGGGCCATGATGTTGGGCAGGCTGCCGATCAGCGGCTGGATCAGCAGGCCGTCAGCCACGGCTTGCCGTTCCTCGGCGCTGAGGTGCGCCCCAAGGGCGTGCAGGCGGTGATGAATGGCGGTGGGCGGGTCTTCGGCGGGCAGGTAGATCACCGGGCCGGTGGGCAGTTCGCCCACCTCCAGCAGATCCGGCCCGCCTGCAATCTGTGCGGCCAGTTGCAGGGCCAGCATGGATTTACCGGCACCACCGGGCGACACCAGCGCCCCGACCGTACCGGCCACCATGTTGGGCAAAACGTAGTCCAGCGGTGGCGGCGCTGCTGCGAACGCCTCCAGAATATTGATAGGCTTATGGGTAGCCATTGATTGCCTCCTTTGCAGGCAGTTGGTGGTTAGGCGCTGGCGGGGTCACTACCCCCGCCCTGCGCCGCTCTGAGTTCTTCCAGGCACTCGCGCAGCGCCTCGTATTCGTCGTCGGTCAGCCAGAACTTGCGCTGACGCATCCCTTTGGCCTTCATGCGCTCGGCATATCGCGCTTGGCGTACAGCGTCAGGGCTGGCCAGCAGGTCGCCGGTCTGCTTGTCCTTTTGGTCTTTCATATCAGTCACCGAGAAACTTGCCGGGGCCGAAAGGCTTGTCTTCGCGGAACAAGGACAAGGTGCAGCCGTCAAGGTTAAGGCTGGCCATATCAGCGACTGAAAAGCGGCCAGCCTCGGCCTTGTTTGACGTATAACCAAAGCCACCGGGCAACCAATAGCCCTTGTCACTTTTGATCAGGTAGACCGACCCTGAAGCGCTTTTTTCGTATTCCATAAAACCCCCTTCTGTGCGTGAGTACTCATAGTATAACAGGCGTGAGTACCAACGCAAGCACTACATGCTGAAATCTGGCCCGCCCCTGTCCATGCCTCGCTGGCGGGGTGCCGGTGCCCGTGCCAGCTCGGCCCGCGCAAGCTGGACGCTGGGCAGACCCATGACCTTGCTGACGGTGCGCTCGATGTAATCCGCTTCGTGGCCGGGCTTGCGCTCTGCCAGCGCTGGGCTGGCCTCGGCCATGGCCTTGCCGATTTCCTCGGCACTGCGGCCCCGGCTGGCCAGCTTCTGCGCGGCGATAAAGTCGCACTTGCTGAGGTCATCACCGAAGCGCTTGACCAGCCCGGCCATCTCGCTGCGGTACTCGTCCAGCGCCGTGCGCCGGTGGCGGCTAAGCTGCCGCTCGGGCAGTTCGAGGCTGGCCAGCCTGCGGGCCTTCTCCTGCTGCCGCTGGGCCTGCTCGATCTGCTGGCCAGCCTGCTGCACCAGCGCCGGGCCAGCGGTGGCGGTCTTGCCCTTGGATTCACGCAGCAGCACCCACGGCTGATAACCGGCGCGGGTGGTGTGCTTGTCCTTGCGGTTGGTGAAGCCCGCCAAGCGGCCATAGTGGCGGCTGTCGGCGCTGGCCGGGTCGGCGTCGTACTCGCTGGCCAGCGTCCGGGCAATCTGCCCCCGAAGTTCACCGCCTGCGGCGTCGGCCACCTTGACCCATGCCTGATAGTTCTTCGGGCTGGTTTCCACTACCAGGGCAGGCTCCCGGCCCTCGGCTTTCATGTCATCCAGGTCAAACTCGCTGAGGTCGTCCACCAGCACCAGACCATGCCGCTCCTGCTCGGCGGGCCTGATATACACGTCATTGCCCTGGGCATTCATCCGCTTGAGCCATGGCGTGTTCTGGAGCACTTCGGCGGCTGACCATTCCCGGTTCATCATCTGGCCGGTGGTGGCGTCCCTGACGCCGATATCGAAGCGCTCACAGCCCATGGCCTTGAGCTGTCGGCCTATGGCCTGCAAAGTCCTGTCGTTCTTCATCGGGCCACCAAGCGATTCCCACACATTATACGAGCCGGAAGCATAAAGTGTAGCTAGATCCGAAGGATGAGCCGGGCTGAATGATCGACCGAGACAGGCCCTGCGGGGCTGCACACGCGCCCCCACCCTTCGGGTAGGGGGAAAGGCCGCTAAAGCGGCTAAAAGCGCTCCAGCGTATTTCTGCGGGGTTTGGTGTGGGGTTTAGCGGGCTTTGCCCGCCTTTCCCCCTGCCGCGCAGCGGTGGGGCGGTGTGTAGCCTAGCGCAGCGAATAGACCAGCTATCCGGCCTCTGGCCGGGCATATTGGGCAAGGGCAGCAGCGCCCCACAAGGGCGCTGATAACCGCGCCTAGTGGATTATTCTTAGATAATCATGGATGGATTTTTCCAACACCCCGCCAGCCCCCGCCCCTGCTGGGTTTGCAGGTTTGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTATTGCAGGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTAGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_16.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_16.10,,Synthetic Biology,,,,2113,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Ampicillin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTAGTAGCCCGCCTAATGAGCGGGCTTTTTTTTAATTCCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCATTCAGCATTTTCGTGTGGCGCTGATTCCGTTTTTTGCGGCGTTTTGCCTGCCGGTGTTTGCGCATCCGGAAACCCTGGTGAAAGTGAAAGATGCGGAAGATCAACTGGGTGCGCGCGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTGGAATCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGCGGTGCGGTTCTGAGCCGTGTGGATGCGGGCCAGGAACAACTGGGCCGTCGTATTCATTATAGCCAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATGACCGTGCGTGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTGCTGCTGACGACCATTGGCGGTCCGAAAGAACTGACCGCGTTTCTGCATAACATGGGCGATCATGTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAACGTGATACCACCATGCCGGCAGCAATGGCGACCACCCTGCGTAAACTGCTGACGGGTGAGCTGCTGACCCTGGCAAGCCGCCAGCAACTGATTGATTGGATGGAAGCGGATAAAGTGGCGGGTCCGCTGCTGCGTAGCGCGCTGCCGGCTGGCTGGTTTATTGCGGATAAAAGCGGTGCGGGCGAACGTGGCAGCCGTGGCATTATTGCGGCGCTGGGCCCGGATGGTAAACCGAGCCGTATTGTGGTGATTTATACCACCGGCAGCCAGGCGACGATGGATGAACGTAACCGTCAGATTGCGGAAATTGGCGCGAGCCTGATTAAACATTGGTAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGACGACCCTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCTAGAAATATTTTATCTGATTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_17.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_17.10,,Synthetic Biology,,,,2841,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Ampicillin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTAGTAGCCCGCCTAATGAGCGGGCTTTTTTTTAATTCCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCATTCAGCATTTTCGTGTGGCGCTGATTCCGTTTTTTGCGGCGTTTTGCCTGCCGGTGTTTGCGCATCCGGAAACCCTGGTGAAAGTGAAAGATGCGGAAGATCAACTGGGTGCGCGCGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTGGAATCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGCGGTGCGGTTCTGAGCCGTGTGGATGCGGGCCAGGAACAACTGGGCCGTCGTATTCATTATAGCCAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATGACCGTGCGTGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTGCTGCTGACGACCATTGGCGGTCCGAAAGAACTGACCGCGTTTCTGCATAACATGGGCGATCATGTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAACGTGATACCACCATGCCGGCAGCAATGGCGACCACCCTGCGTAAACTGCTGACGGGTGAGCTGCTGACCCTGGCAAGCCGCCAGCAACTGATTGATTGGATGGAAGCGGATAAAGTGGCGGGTCCGCTGCTGCGTAGCGCGCTGCCGGCTGGCTGGTTTATTGCGGATAAAAGCGGTGCGGGCGAACGTGGCAGCCGTGGCATTATTGCGGCGCTGGGCCCGGATGGTAAACCGAGCCGTATTGTGGTGATTTATACCACCGGCAGCCAGGCGACGATGGATGAACGTAACCGTCAGATTGCGGAAATTGGCGCGAGCCTGATTAAACATTGGTAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGACGACCCTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_19.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_19.10,,Synthetic Biology,,,,2766,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Ampicillin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTAGTAGCCCGCCTAATGAGCGGGCTTTTTTTTAATTCCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCATTCAGCATTTTCGTGTGGCGCTGATTCCGTTTTTTGCGGCGTTTTGCCTGCCGGTGTTTGCGCATCCGGAAACCCTGGTGAAAGTGAAAGATGCGGAAGATCAACTGGGTGCGCGCGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTGGAATCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGCGGTGCGGTTCTGAGCCGTGTGGATGCGGGCCAGGAACAACTGGGCCGTCGTATTCATTATAGCCAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATGACCGTGCGTGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTGCTGCTGACGACCATTGGCGGTCCGAAAGAACTGACCGCGTTTCTGCATAACATGGGCGATCATGTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAACGTGATACCACCATGCCGGCAGCAATGGCGACCACCCTGCGTAAACTGCTGACGGGTGAGCTGCTGACCCTGGCAAGCCGCCAGCAACTGATTGATTGGATGGAAGCGGATAAAGTGGCGGGTCCGCTGCTGCGTAGCGCGCTGCCGGCTGGCTGGTTTATTGCGGATAAAAGCGGTGCGGGCGAACGTGGCAGCCGTGGCATTATTGCGGCGCTGGGCCCGGATGGTAAACCGAGCCGTATTGTGGTGATTTATACCACCGGCAGCCAGGCGACGATGGATGAACGTAACCGTCAGATTGCGGAAATTGGCGCGAGCCTGATTAAACATTGGTAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGACGACCCTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGCAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_17_pKD46.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_17_pKD46.10,,Synthetic Biology,,,,2910,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Ampicillin,,,,Low Copy,30 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTAGTAGCCCGCCTAATGAGCGGGCTTTTTTTTAATTCCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGCATTCAGCATTTTCGTGTGGCGCTGATTCCGTTTTTTGCGGCGTTTTGCCTGCCGGTGTTTGCGCATCCGGAAACCCTGGTGAAAGTGAAAGATGCGGAAGATCAACTGGGTGCGCGCGTGGGCTATATTGAACTGGATCTGAACAGCGGCAAAATTCTGGAATCTTTTCGTCCGGAAGAACGTTTTCCGATGATGAGCACCTTTAAAGTGCTGCTGTGCGGTGCGGTTCTGAGCCGTGTGGATGCGGGCCAGGAACAACTGGGCCGTCGTATTCATTATAGCCAGAACGATCTGGTGGAATATAGCCCGGTGACCGAAAAACATCTGACCGATGGCATGACCGTGCGTGAACTGTGCAGCGCGGCGATTACCATGAGCGATAACACCGCGGCGAACCTGCTGCTGACGACCATTGGCGGTCCGAAAGAACTGACCGCGTTTCTGCATAACATGGGCGATCATGTGACCCGTCTGGATCGTTGGGAACCGGAACTGAACGAAGCGATTCCGAACGATGAACGTGATACCACCATGCCGGCAGCAATGGCGACCACCCTGCGTAAACTGCTGACGGGTGAGCTGCTGACCCTGGCAAGCCGCCAGCAACTGATTGATTGGATGGAAGCGGATAAAGTGGCGGGTCCGCTGCTGCGTAGCGCGCTGCCGGCTGGCTGGTTTATTGCGGATAAAAGCGGTGCGGGCGAACGTGGCAGCCGTGGCATTATTGCGGCGCTGGGCCCGGATGGTAAACCGAGCCGTATTGTGGTGATTTATACCACCGGCAGCCAGGCGACGATGGATGAACGTAACCGTCAGATTGCGGAAATTGGCGCGAGCCTGATTAAACATTGGTAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGACGACCCTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCTGTTCTACTTTTGTTTGTTAGTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTACGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGTTGCGGCCGCAATCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_25a.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_25a.10,,Synthetic Biology,,,,4943,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Kanamycin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAGCGTGAAACGAGCTGTAGCCGTCCGCGTCTGAACAGCAACATGGATGCGGATCTGTATGGCTATAAATGGGCGCGTGATAACGTGGGTCAGAGCGGCGCGACCATTTATCGTCTGTATGGCAAACCGGATGCGCCGGAACTGTTTCTGAAACATGGCAAAGGCAGCGTGGCGAACGATGTGACCGATGAAATGGTGCGTCTGAACTGGCTGACCGAATTTATGCCGCTGCCGACCATTAAACATTTTATTCGCACCCCGGATGATGCGTGGCTGCTGACCACCGCGATTCCGGGCAAAACCGCGTTTCAGGTGCTGGAAGAATATCCGGATAGCGGCGAAAACATTGTGGATGCGCTGGCCGTGTTTCTGCGTCGTCTGCATAGCATTCCGGTGTGCAACTGCCCGTTTAACAGCGATCGTGTGTTTCGTCTGGCCCAGGCGCAGAGCCGTATGAACAACGGCCTGGTGGATGCGAGCGATTTTGATGATGAACGTAACGGCTGGCCGGTGGAACAGGTGTGGAAAGAAATGCATAAACTGCTGCCGTTTAGCCCGGATAGCGTGGTGACCCACGGCGATTTTAGCCTGGATAACCTGATTTTCGATGAAGGCAAACTGATTGGCTGCATTGATGTGGGCCGTGTGGGCATTGCGGATCGTTATCAGGATCTGGCCATTCTGTGGAACTGCCTGGGCGAATTTAGCCCGAGCCTGCAAAAACGTCTGTTTCAGAAATATGGCATTGATAATCCGGATATGAACAAACTGCAATTTCATCTGATGCTGGATGAATTTTTCTAATAATTAATTGGACCGCGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGTAGGCCGGCCTCAGCCTGCCGCCTTGGGCCGGGTGATGTCGTACTTGCCCGCCGCGAACTCGGTTACCGTCCAGCCCAGCGCGACCAGCTCCGGCAACGCCTCGCGCACCCGCTGGCGGCGCTTGCGCATGGTCGAACCACTGGCCTCTGACGGCCAGACATAGCCGCACAAGGTATCTATGGAAGCCTTGCCGGTTTTGCCGGGGTCGATCCAGCCACACAGCCGCTGGTGCAGCAGGCGGGCGGTTTCGCTGTCCAGCGCCCGCACCTCGTCCATGCTGATGCGCACATGCTGGCCGCCACCCATGACGGCCTGCGCGATCAAGGGGTTCAGGGCCACGTACAGGCGCCCGTCCGCCTCGTCGCTGGCGTACTCCGACAGCAGCCGAAACCCCTGCCGCTTGCGGCCATTCTGGGCGATGATGGATACCTTCCAAAGGCGCTCGATGCAGTCCTGTATGTGCTTGAGCGCCCCACCACTATCGACCTCTGCCCCGATTTCCTTTGCCAGCGCCCGATAGCTACCTTTGACCACCATGGCATCAGCGGTGACGGCCTCCCACTTGGGTTCCAGGAACAGCCGGAGCTGCCGTCCGCCTTCGGTCTTGGGTTCCGGGCCAAGCACTAGGCCATTAGGCCCAGCCATGGCCACCAGCCCTTGCAGGATGCGCAGATCATCAGCGCCCAGCGGCTCCGGGCCGCTGAACTCGATCCGCTTGCCGTCGCCGTAGTCATACGTCACGTCCAGCTTGCTGCGCTTGCGCTCGCCCCGCTTGAGGGCACGGAACAGGCCGGGGGCCAGACAGTGCGCCGGGTCGTGCCGGACGTGGCTGAGGCTGTGCTTGTTCTTAGGCTTCACCACGGGGCACCCCCTTGCTCTTGCGCTGCCTCTCCAGCACGGCGGGCTTGAGCACCCCGCCGTCATGCCGCCTGAACCACCGATCAGCGAACGGTGCGCCATAGTTGGCCTTGCTCACACCGAAGCGGACGAAGAACCGGCGCTGGTCGTCGTCCACACCCCATTCCTCGGCCTCGGCGCTGGTCATGCTCGACAGGTAGGACTGCCAGCGGATGTTATCGACCAGTACCGAGCTGCCCCGGCTGGCCTGCTGCTGGTCGCCTGCGCCCATCATGGCCGCGCCCTTGCTGGCATGGTGCAGGAACACGATAGAGCACCCGGTATCGGCGGCGATGGCCTCCATGCGACCGATGACCTGGGCCATGGGGCCGCTGGCGTTTTCTTCCTCGATGTGGAACCGGCGCAGCGTGTCCAGCACCATCAGGCGGCGGCCCTCGGCGGCGCGCTTGAGGCCGTCGAACCACTCCGGGGCCATGATGTTGGGCAGGCTGCCGATCAGCGGCTGGATCAGCAGGCCGTCAGCCACGGCTTGCCGTTCCTCGGCGCTGAGGTGCGCCCCAAGGGCGTGCAGGCGGTGATGAATGGCGGTGGGCGGGTCTTCGGCGGGCAGGTAGATCACCGGGCCGGTGGGCAGTTCGCCCACCTCCAGCAGATCCGGCCCGCCTGCAATCTGTGCGGCCAGTTGCAGGGCCAGCATGGATTTACCGGCACCACCGGGCGACACCAGCGCCCCGACCGTACCGGCCACCATGTTGGGCAAAACGTAGTCCAGCGGTGGCGGCGCTGCTGCGAACGCCTCCAGAATATTGATAGGCTTATGGGTAGCCATTGATTGCCTCCTTTGCAGGCAGTTGGTGGTTAGGCGCTGGCGGGGTCACTACCCCCGCCCTGCGCCGCTCTGAGTTCTTCCAGGCACTCGCGCAGCGCCTCGTATTCGTCGTCGGTCAGCCAGAACTTGCGCTGACGCATCCCTTTGGCCTTCATGCGCTCGGCATATCGCGCTTGGCGTACAGCGTCAGGGCTGGCCAGCAGGTCGCCGGTCTGCTTGTCCTTTTGGTCTTTCATATCAGTCACCGAGAAACTTGCCGGGGCCGAAAGGCTTGTCTTCGCGGAACAAGGACAAGGTGCAGCCGTCAAGGTTAAGGCTGGCCATATCAGCGACTGAAAAGCGGCCAGCCTCGGCCTTGTTTGACGTATAACCAAAGCCACCGGGCAACCAATAGCCCTTGTCACTTTTGATCAGGTAGACCGACCCTGAAGCGCTTTTTTCGTATTCCATAAAACCCCCTTCTGTGCGTGAGTACTCATAGTATAACAGGCGTGAGTACCAACGCAAGCACTACATGCTGAAATCTGGCCCGCCCCTGTCCATGCCTCGCTGGCGGGGTGCCGGTGCCCGTGCCAGCTCGGCCCGCGCAAGCTGGACGCTGGGCAGACCCATGACCTTGCTGACGGTGCGCTCGATGTAATCCGCTTCGTGGCCGGGCTTGCGCTCTGCCAGCGCTGGGCTGGCCTCGGCCATGGCCTTGCCGATTTCCTCGGCACTGCGGCCCCGGCTGGCCAGCTTCTGCGCGGCGATAAAGTCGCACTTGCTGAGGTCATCACCGAAGCGCTTGACCAGCCCGGCCATCTCGCTGCGGTACTCGTCCAGCGCCGTGCGCCGGTGGCGGCTAAGCTGCCGCTCGGGCAGTTCGAGGCTGGCCAGCCTGCGGGCCTTCTCCTGCTGCCGCTGGGCCTGCTCGATCTGCTGGCCAGCCTGCTGCACCAGCGCCGGGCCAGCGGTGGCGGTCTTGCCCTTGGATTCACGCAGCAGCACCCACGGCTGATAACCGGCGCGGGTGGTGTGCTTGTCCTTGCGGTTGGTGAAGCCCGCCAAGCGGCCATAGTGGCGGCTGTCGGCGCTGGCCGGGTCGGCGTCGTACTCGCTGGCCAGCGTCCGGGCAATCTGCCCCCGAAGTTCACCGCCTGCGGCGTCGGCCACCTTGACCCATGCCTGATAGTTCTTCGGGCTGGTTTCCACTACCAGGGCAGGCTCCCGGCCCTCGGCTTTCATGTCATCCAGGTCAAACTCGCTGAGGTCGTCCACCAGCACCAGACCATGCCGCTCCTGCTCGGCGGGCCTGATATACACGTCATTGCCCTGGGCATTCATCCGCTTGAGCCATGGCGTGTTCTGGAGCACTTCGGCGGCTGACCATTCCCGGTTCATCATCTGGCCGGTGGTGGCGTCCCTGACGCCGATATCGAAGCGCTCACAGCCCATGGCCTTGAGCTGTCGGCCTATGGCCTGCAAAGTCCTGTCGTTCTTCATCGGGCCACCAAGCGATTCCCACACATTATACGAGCCGGAAGCATAAAGTGTAGCTAGATCCGAAGGATGAGCCGGGCTGAATGATCGACCGAGACAGGCCCTGCGGGGCTGCACACGCGCCCCCACCCTTCGGGTAGGGGGAAAGGCCGCTAAAGCGGCTAAAAGCGCTCCAGCGTATTTCTGCGGGGTTTGGTGTGGGGTTTAGCGGGCTTTGCCCGCCTTTCCCCCTGCCGCGCAGCGGTGGGGCGGTGTGTAGCCTAGCGCAGCGAATAGACCAGCTATCCGGCCTCTGGCCGGGCATATTGGGCAAGGGCAGCAGCGCCCCACAAGGGCGCTGATAACCGCGCCTAGTGGATTATTCTTAGATAATCATGGATGGATTTTTCCAACACCCCGCCAGCCCCCGCCCCTGCTGGGTTTGCAGGTTTGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTATTGCAGGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTAGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_26.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_26.10,,Synthetic Biology,,,,2001,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Kanamycin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAGCGTGAAACGAGCTGTAGCCGTCCGCGTCTGAACAGCAACATGGATGCGGATCTGTATGGCTATAAATGGGCGCGTGATAACGTGGGTCAGAGCGGCGCGACCATTTATCGTCTGTATGGCAAACCGGATGCGCCGGAACTGTTTCTGAAACATGGCAAAGGCAGCGTGGCGAACGATGTGACCGATGAAATGGTGCGTCTGAACTGGCTGACCGAATTTATGCCGCTGCCGACCATTAAACATTTTATTCGCACCCCGGATGATGCGTGGCTGCTGACCACCGCGATTCCGGGCAAAACCGCGTTTCAGGTGCTGGAAGAATATCCGGATAGCGGCGAAAACATTGTGGATGCGCTGGCCGTGTTTCTGCGTCGTCTGCATAGCATTCCGGTGTGCAACTGCCCGTTTAACAGCGATCGTGTGTTTCGTCTGGCCCAGGCGCAGAGCCGTATGAACAACGGCCTGGTGGATGCGAGCGATTTTGATGATGAACGTAACGGCTGGCCGGTGGAACAGGTGTGGAAAGAAATGCATAAACTGCTGCCGTTTAGCCCGGATAGCGTGGTGACCCACGGCGATTTTAGCCTGGATAACCTGATTTTCGATGAAGGCAAACTGATTGGCTGCATTGATGTGGGCCGTGTGGGCATTGCGGATCGTTATCAGGATCTGGCCATTCTGTGGAACTGCCTGGGCGAATTTAGCCCGAGCCTGCAAAAACGTCTGTTTCAGAAATATGGCATTGATAATCCGGATATGAACAAACTGCAATTTCATCTGATGCTGGATGAATTTTTCTAATAATTAATTGGACCGCGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCTAGAAATATTTTATCTGATTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_27.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_27.10,,Synthetic Biology,,,,2729,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Kanamycin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAGCGTGAAACGAGCTGTAGCCGTCCGCGTCTGAACAGCAACATGGATGCGGATCTGTATGGCTATAAATGGGCGCGTGATAACGTGGGTCAGAGCGGCGCGACCATTTATCGTCTGTATGGCAAACCGGATGCGCCGGAACTGTTTCTGAAACATGGCAAAGGCAGCGTGGCGAACGATGTGACCGATGAAATGGTGCGTCTGAACTGGCTGACCGAATTTATGCCGCTGCCGACCATTAAACATTTTATTCGCACCCCGGATGATGCGTGGCTGCTGACCACCGCGATTCCGGGCAAAACCGCGTTTCAGGTGCTGGAAGAATATCCGGATAGCGGCGAAAACATTGTGGATGCGCTGGCCGTGTTTCTGCGTCGTCTGCATAGCATTCCGGTGTGCAACTGCCCGTTTAACAGCGATCGTGTGTTTCGTCTGGCCCAGGCGCAGAGCCGTATGAACAACGGCCTGGTGGATGCGAGCGATTTTGATGATGAACGTAACGGCTGGCCGGTGGAACAGGTGTGGAAAGAAATGCATAAACTGCTGCCGTTTAGCCCGGATAGCGTGGTGACCCACGGCGATTTTAGCCTGGATAACCTGATTTTCGATGAAGGCAAACTGATTGGCTGCATTGATGTGGGCCGTGTGGGCATTGCGGATCGTTATCAGGATCTGGCCATTCTGTGGAACTGCCTGGGCGAATTTAGCCCGAGCCTGCAAAAACGTCTGTTTCAGAAATATGGCATTGATAATCCGGATATGAACAAACTGCAATTTCATCTGATGCTGGATGAATTTTTCTAATAATTAATTGGACCGCGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_29.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_29.10,,Synthetic Biology,,,,2654,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Kanamycin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAGCGTGAAACGAGCTGTAGCCGTCCGCGTCTGAACAGCAACATGGATGCGGATCTGTATGGCTATAAATGGGCGCGTGATAACGTGGGTCAGAGCGGCGCGACCATTTATCGTCTGTATGGCAAACCGGATGCGCCGGAACTGTTTCTGAAACATGGCAAAGGCAGCGTGGCGAACGATGTGACCGATGAAATGGTGCGTCTGAACTGGCTGACCGAATTTATGCCGCTGCCGACCATTAAACATTTTATTCGCACCCCGGATGATGCGTGGCTGCTGACCACCGCGATTCCGGGCAAAACCGCGTTTCAGGTGCTGGAAGAATATCCGGATAGCGGCGAAAACATTGTGGATGCGCTGGCCGTGTTTCTGCGTCGTCTGCATAGCATTCCGGTGTGCAACTGCCCGTTTAACAGCGATCGTGTGTTTCGTCTGGCCCAGGCGCAGAGCCGTATGAACAACGGCCTGGTGGATGCGAGCGATTTTGATGATGAACGTAACGGCTGGCCGGTGGAACAGGTGTGGAAAGAAATGCATAAACTGCTGCCGTTTAGCCCGGATAGCGTGGTGACCCACGGCGATTTTAGCCTGGATAACCTGATTTTCGATGAAGGCAAACTGATTGGCTGCATTGATGTGGGCCGTGTGGGCATTGCGGATCGTTATCAGGATCTGGCCATTCTGTGGAACTGCCTGGGCGAATTTAGCCCGAGCCTGCAAAAACGTCTGTTTCAGAAATATGGCATTGATAATCCGGATATGAACAAACTGCAATTTCATCTGATGCTGGATGAATTTTTCTAATAATTAATTGGACCGCGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGCAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_27_pKD46.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_27_pKD46.10,,Synthetic Biology,,,,2798,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Kanamycin,,,,Low Copy,30 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGTGTCTCAAAATCTCTGATGTTACATTGCACAAGATAAAAATATATCATCATGAACAATAAAACTGTCTGCTTACATAAACAGTAATACAAGGGGTGTTATGAGCCATATTCAGCGTGAAACGAGCTGTAGCCGTCCGCGTCTGAACAGCAACATGGATGCGGATCTGTATGGCTATAAATGGGCGCGTGATAACGTGGGTCAGAGCGGCGCGACCATTTATCGTCTGTATGGCAAACCGGATGCGCCGGAACTGTTTCTGAAACATGGCAAAGGCAGCGTGGCGAACGATGTGACCGATGAAATGGTGCGTCTGAACTGGCTGACCGAATTTATGCCGCTGCCGACCATTAAACATTTTATTCGCACCCCGGATGATGCGTGGCTGCTGACCACCGCGATTCCGGGCAAAACCGCGTTTCAGGTGCTGGAAGAATATCCGGATAGCGGCGAAAACATTGTGGATGCGCTGGCCGTGTTTCTGCGTCGTCTGCATAGCATTCCGGTGTGCAACTGCCCGTTTAACAGCGATCGTGTGTTTCGTCTGGCCCAGGCGCAGAGCCGTATGAACAACGGCCTGGTGGATGCGAGCGATTTTGATGATGAACGTAACGGCTGGCCGGTGGAACAGGTGTGGAAAGAAATGCATAAACTGCTGCCGTTTAGCCCGGATAGCGTGGTGACCCACGGCGATTTTAGCCTGGATAACCTGATTTTCGATGAAGGCAAACTGATTGGCTGCATTGATGTGGGCCGTGTGGGCATTGCGGATCGTTATCAGGATCTGGCCATTCTGTGGAACTGCCTGGGCGAATTTAGCCCGAGCCTGCAAAAACGTCTGTTTCAGAAATATGGCATTGATAATCCGGATATGAACAAACTGCAATTTCATCTGATGCTGGATGAATTTTTCTAATAATTAATTGGACCGCGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCTGTTCTACTTTTGTTTGTTAGTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTACGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGTTGCGGCCGCAATCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_35a.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_35a.10,,Synthetic Biology,,,,4799,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Chloramphenicol,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTGGCGAAAATGAGACGTTGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTGACTTTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGTAGGCCGGCCTCAGCCTGCCGCCTTGGGCCGGGTGATGTCGTACTTGCCCGCCGCGAACTCGGTTACCGTCCAGCCCAGCGCGACCAGCTCCGGCAACGCCTCGCGCACCCGCTGGCGGCGCTTGCGCATGGTCGAACCACTGGCCTCTGACGGCCAGACATAGCCGCACAAGGTATCTATGGAAGCCTTGCCGGTTTTGCCGGGGTCGATCCAGCCACACAGCCGCTGGTGCAGCAGGCGGGCGGTTTCGCTGTCCAGCGCCCGCACCTCGTCCATGCTGATGCGCACATGCTGGCCGCCACCCATGACGGCCTGCGCGATCAAGGGGTTCAGGGCCACGTACAGGCGCCCGTCCGCCTCGTCGCTGGCGTACTCCGACAGCAGCCGAAACCCCTGCCGCTTGCGGCCATTCTGGGCGATGATGGATACCTTCCAAAGGCGCTCGATGCAGTCCTGTATGTGCTTGAGCGCCCCACCACTATCGACCTCTGCCCCGATTTCCTTTGCCAGCGCCCGATAGCTACCTTTGACCACCATGGCATCAGCGGTGACGGCCTCCCACTTGGGTTCCAGGAACAGCCGGAGCTGCCGTCCGCCTTCGGTCTTGGGTTCCGGGCCAAGCACTAGGCCATTAGGCCCAGCCATGGCCACCAGCCCTTGCAGGATGCGCAGATCATCAGCGCCCAGCGGCTCCGGGCCGCTGAACTCGATCCGCTTGCCGTCGCCGTAGTCATACGTCACGTCCAGCTTGCTGCGCTTGCGCTCGCCCCGCTTGAGGGCACGGAACAGGCCGGGGGCCAGACAGTGCGCCGGGTCGTGCCGGACGTGGCTGAGGCTGTGCTTGTTCTTAGGCTTCACCACGGGGCACCCCCTTGCTCTTGCGCTGCCTCTCCAGCACGGCGGGCTTGAGCACCCCGCCGTCATGCCGCCTGAACCACCGATCAGCGAACGGTGCGCCATAGTTGGCCTTGCTCACACCGAAGCGGACGAAGAACCGGCGCTGGTCGTCGTCCACACCCCATTCCTCGGCCTCGGCGCTGGTCATGCTCGACAGGTAGGACTGCCAGCGGATGTTATCGACCAGTACCGAGCTGCCCCGGCTGGCCTGCTGCTGGTCGCCTGCGCCCATCATGGCCGCGCCCTTGCTGGCATGGTGCAGGAACACGATAGAGCACCCGGTATCGGCGGCGATGGCCTCCATGCGACCGATGACCTGGGCCATGGGGCCGCTGGCGTTTTCTTCCTCGATGTGGAACCGGCGCAGCGTGTCCAGCACCATCAGGCGGCGGCCCTCGGCGGCGCGCTTGAGGCCGTCGAACCACTCCGGGGCCATGATGTTGGGCAGGCTGCCGATCAGCGGCTGGATCAGCAGGCCGTCAGCCACGGCTTGCCGTTCCTCGGCGCTGAGGTGCGCCCCAAGGGCGTGCAGGCGGTGATGAATGGCGGTGGGCGGGTCTTCGGCGGGCAGGTAGATCACCGGGCCGGTGGGCAGTTCGCCCACCTCCAGCAGATCCGGCCCGCCTGCAATCTGTGCGGCCAGTTGCAGGGCCAGCATGGATTTACCGGCACCACCGGGCGACACCAGCGCCCCGACCGTACCGGCCACCATGTTGGGCAAAACGTAGTCCAGCGGTGGCGGCGCTGCTGCGAACGCCTCCAGAATATTGATAGGCTTATGGGTAGCCATTGATTGCCTCCTTTGCAGGCAGTTGGTGGTTAGGCGCTGGCGGGGTCACTACCCCCGCCCTGCGCCGCTCTGAGTTCTTCCAGGCACTCGCGCAGCGCCTCGTATTCGTCGTCGGTCAGCCAGAACTTGCGCTGACGCATCCCTTTGGCCTTCATGCGCTCGGCATATCGCGCTTGGCGTACAGCGTCAGGGCTGGCCAGCAGGTCGCCGGTCTGCTTGTCCTTTTGGTCTTTCATATCAGTCACCGAGAAACTTGCCGGGGCCGAAAGGCTTGTCTTCGCGGAACAAGGACAAGGTGCAGCCGTCAAGGTTAAGGCTGGCCATATCAGCGACTGAAAAGCGGCCAGCCTCGGCCTTGTTTGACGTATAACCAAAGCCACCGGGCAACCAATAGCCCTTGTCACTTTTGATCAGGTAGACCGACCCTGAAGCGCTTTTTTCGTATTCCATAAAACCCCCTTCTGTGCGTGAGTACTCATAGTATAACAGGCGTGAGTACCAACGCAAGCACTACATGCTGAAATCTGGCCCGCCCCTGTCCATGCCTCGCTGGCGGGGTGCCGGTGCCCGTGCCAGCTCGGCCCGCGCAAGCTGGACGCTGGGCAGACCCATGACCTTGCTGACGGTGCGCTCGATGTAATCCGCTTCGTGGCCGGGCTTGCGCTCTGCCAGCGCTGGGCTGGCCTCGGCCATGGCCTTGCCGATTTCCTCGGCACTGCGGCCCCGGCTGGCCAGCTTCTGCGCGGCGATAAAGTCGCACTTGCTGAGGTCATCACCGAAGCGCTTGACCAGCCCGGCCATCTCGCTGCGGTACTCGTCCAGCGCCGTGCGCCGGTGGCGGCTAAGCTGCCGCTCGGGCAGTTCGAGGCTGGCCAGCCTGCGGGCCTTCTCCTGCTGCCGCTGGGCCTGCTCGATCTGCTGGCCAGCCTGCTGCACCAGCGCCGGGCCAGCGGTGGCGGTCTTGCCCTTGGATTCACGCAGCAGCACCCACGGCTGATAACCGGCGCGGGTGGTGTGCTTGTCCTTGCGGTTGGTGAAGCCCGCCAAGCGGCCATAGTGGCGGCTGTCGGCGCTGGCCGGGTCGGCGTCGTACTCGCTGGCCAGCGTCCGGGCAATCTGCCCCCGAAGTTCACCGCCTGCGGCGTCGGCCACCTTGACCCATGCCTGATAGTTCTTCGGGCTGGTTTCCACTACCAGGGCAGGCTCCCGGCCCTCGGCTTTCATGTCATCCAGGTCAAACTCGCTGAGGTCGTCCACCAGCACCAGACCATGCCGCTCCTGCTCGGCGGGCCTGATATACACGTCATTGCCCTGGGCATTCATCCGCTTGAGCCATGGCGTGTTCTGGAGCACTTCGGCGGCTGACCATTCCCGGTTCATCATCTGGCCGGTGGTGGCGTCCCTGACGCCGATATCGAAGCGCTCACAGCCCATGGCCTTGAGCTGTCGGCCTATGGCCTGCAAAGTCCTGTCGTTCTTCATCGGGCCACCAAGCGATTCCCACACATTATACGAGCCGGAAGCATAAAGTGTAGCTAGATCCGAAGGATGAGCCGGGCTGAATGATCGACCGAGACAGGCCCTGCGGGGCTGCACACGCGCCCCCACCCTTCGGGTAGGGGGAAAGGCCGCTAAAGCGGCTAAAAGCGCTCCAGCGTATTTCTGCGGGGTTTGGTGTGGGGTTTAGCGGGCTTTGCCCGCCTTTCCCCCTGCCGCGCAGCGGTGGGGCGGTGTGTAGCCTAGCGCAGCGAATAGACCAGCTATCCGGCCTCTGGCCGGGCATATTGGGCAAGGGCAGCAGCGCCCCACAAGGGCGCTGATAACCGCGCCTAGTGGATTATTCTTAGATAATCATGGATGGATTTTTCCAACACCCCGCCAGCCCCCGCCCCTGCTGGGTTTGCAGGTTTGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTATTGCAGGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTAGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_36.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_36.10,,Synthetic Biology,,,,1857,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Chloramphenicol,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTGGCGAAAATGAGACGTTGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTGACTTTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCTAGAAATATTTTATCTGATTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_37.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_37.10,,Synthetic Biology,,,,2585,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Chloramphenicol,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTGGCGAAAATGAGACGTTGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTGACTTTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_39.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_39.10,,Synthetic Biology,,,,2510,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Chloramphenicol,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTGGCGAAAATGAGACGTTGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTGACTTTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGCAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_37_pKD46.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_37_pKD46.10,,Synthetic Biology,,,,2654,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Chloramphenicol,,,,Low Copy,30 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTGGCGAAAATGAGACGTTGATCGGCACGTAAGAGGTTCCAACTTTCACCATAATGAAATAAGATCACTACCGGGCGTATTTTTTGAGTTATCGAGATTTTCAGGAGCTAAGGAAGCTAAAATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTTCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTTTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAATTTGACTTTTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCTGTTCTACTTTTGTTTGTTAGTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTACGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGTTGCGGCCGCAATCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_45a.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_45a.10,,Synthetic Biology,,,,5013,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Spectinomycin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGTAGGCCGGCCTCAGCCTGCCGCCTTGGGCCGGGTGATGTCGTACTTGCCCGCCGCGAACTCGGTTACCGTCCAGCCCAGCGCGACCAGCTCCGGCAACGCCTCGCGCACCCGCTGGCGGCGCTTGCGCATGGTCGAACCACTGGCCTCTGACGGCCAGACATAGCCGCACAAGGTATCTATGGAAGCCTTGCCGGTTTTGCCGGGGTCGATCCAGCCACACAGCCGCTGGTGCAGCAGGCGGGCGGTTTCGCTGTCCAGCGCCCGCACCTCGTCCATGCTGATGCGCACATGCTGGCCGCCACCCATGACGGCCTGCGCGATCAAGGGGTTCAGGGCCACGTACAGGCGCCCGTCCGCCTCGTCGCTGGCGTACTCCGACAGCAGCCGAAACCCCTGCCGCTTGCGGCCATTCTGGGCGATGATGGATACCTTCCAAAGGCGCTCGATGCAGTCCTGTATGTGCTTGAGCGCCCCACCACTATCGACCTCTGCCCCGATTTCCTTTGCCAGCGCCCGATAGCTACCTTTGACCACCATGGCATCAGCGGTGACGGCCTCCCACTTGGGTTCCAGGAACAGCCGGAGCTGCCGTCCGCCTTCGGTCTTGGGTTCCGGGCCAAGCACTAGGCCATTAGGCCCAGCCATGGCCACCAGCCCTTGCAGGATGCGCAGATCATCAGCGCCCAGCGGCTCCGGGCCGCTGAACTCGATCCGCTTGCCGTCGCCGTAGTCATACGTCACGTCCAGCTTGCTGCGCTTGCGCTCGCCCCGCTTGAGGGCACGGAACAGGCCGGGGGCCAGACAGTGCGCCGGGTCGTGCCGGACGTGGCTGAGGCTGTGCTTGTTCTTAGGCTTCACCACGGGGCACCCCCTTGCTCTTGCGCTGCCTCTCCAGCACGGCGGGCTTGAGCACCCCGCCGTCATGCCGCCTGAACCACCGATCAGCGAACGGTGCGCCATAGTTGGCCTTGCTCACACCGAAGCGGACGAAGAACCGGCGCTGGTCGTCGTCCACACCCCATTCCTCGGCCTCGGCGCTGGTCATGCTCGACAGGTAGGACTGCCAGCGGATGTTATCGACCAGTACCGAGCTGCCCCGGCTGGCCTGCTGCTGGTCGCCTGCGCCCATCATGGCCGCGCCCTTGCTGGCATGGTGCAGGAACACGATAGAGCACCCGGTATCGGCGGCGATGGCCTCCATGCGACCGATGACCTGGGCCATGGGGCCGCTGGCGTTTTCTTCCTCGATGTGGAACCGGCGCAGCGTGTCCAGCACCATCAGGCGGCGGCCCTCGGCGGCGCGCTTGAGGCCGTCGAACCACTCCGGGGCCATGATGTTGGGCAGGCTGCCGATCAGCGGCTGGATCAGCAGGCCGTCAGCCACGGCTTGCCGTTCCTCGGCGCTGAGGTGCGCCCCAAGGGCGTGCAGGCGGTGATGAATGGCGGTGGGCGGGTCTTCGGCGGGCAGGTAGATCACCGGGCCGGTGGGCAGTTCGCCCACCTCCAGCAGATCCGGCCCGCCTGCAATCTGTGCGGCCAGTTGCAGGGCCAGCATGGATTTACCGGCACCACCGGGCGACACCAGCGCCCCGACCGTACCGGCCACCATGTTGGGCAAAACGTAGTCCAGCGGTGGCGGCGCTGCTGCGAACGCCTCCAGAATATTGATAGGCTTATGGGTAGCCATTGATTGCCTCCTTTGCAGGCAGTTGGTGGTTAGGCGCTGGCGGGGTCACTACCCCCGCCCTGCGCCGCTCTGAGTTCTTCCAGGCACTCGCGCAGCGCCTCGTATTCGTCGTCGGTCAGCCAGAACTTGCGCTGACGCATCCCTTTGGCCTTCATGCGCTCGGCATATCGCGCTTGGCGTACAGCGTCAGGGCTGGCCAGCAGGTCGCCGGTCTGCTTGTCCTTTTGGTCTTTCATATCAGTCACCGAGAAACTTGCCGGGGCCGAAAGGCTTGTCTTCGCGGAACAAGGACAAGGTGCAGCCGTCAAGGTTAAGGCTGGCCATATCAGCGACTGAAAAGCGGCCAGCCTCGGCCTTGTTTGACGTATAACCAAAGCCACCGGGCAACCAATAGCCCTTGTCACTTTTGATCAGGTAGACCGACCCTGAAGCGCTTTTTTCGTATTCCATAAAACCCCCTTCTGTGCGTGAGTACTCATAGTATAACAGGCGTGAGTACCAACGCAAGCACTACATGCTGAAATCTGGCCCGCCCCTGTCCATGCCTCGCTGGCGGGGTGCCGGTGCCCGTGCCAGCTCGGCCCGCGCAAGCTGGACGCTGGGCAGACCCATGACCTTGCTGACGGTGCGCTCGATGTAATCCGCTTCGTGGCCGGGCTTGCGCTCTGCCAGCGCTGGGCTGGCCTCGGCCATGGCCTTGCCGATTTCCTCGGCACTGCGGCCCCGGCTGGCCAGCTTCTGCGCGGCGATAAAGTCGCACTTGCTGAGGTCATCACCGAAGCGCTTGACCAGCCCGGCCATCTCGCTGCGGTACTCGTCCAGCGCCGTGCGCCGGTGGCGGCTAAGCTGCCGCTCGGGCAGTTCGAGGCTGGCCAGCCTGCGGGCCTTCTCCTGCTGCCGCTGGGCCTGCTCGATCTGCTGGCCAGCCTGCTGCACCAGCGCCGGGCCAGCGGTGGCGGTCTTGCCCTTGGATTCACGCAGCAGCACCCACGGCTGATAACCGGCGCGGGTGGTGTGCTTGTCCTTGCGGTTGGTGAAGCCCGCCAAGCGGCCATAGTGGCGGCTGTCGGCGCTGGCCGGGTCGGCGTCGTACTCGCTGGCCAGCGTCCGGGCAATCTGCCCCCGAAGTTCACCGCCTGCGGCGTCGGCCACCTTGACCCATGCCTGATAGTTCTTCGGGCTGGTTTCCACTACCAGGGCAGGCTCCCGGCCCTCGGCTTTCATGTCATCCAGGTCAAACTCGCTGAGGTCGTCCACCAGCACCAGACCATGCCGCTCCTGCTCGGCGGGCCTGATATACACGTCATTGCCCTGGGCATTCATCCGCTTGAGCCATGGCGTGTTCTGGAGCACTTCGGCGGCTGACCATTCCCGGTTCATCATCTGGCCGGTGGTGGCGTCCCTGACGCCGATATCGAAGCGCTCACAGCCCATGGCCTTGAGCTGTCGGCCTATGGCCTGCAAAGTCCTGTCGTTCTTCATCGGGCCACCAAGCGATTCCCACACATTATACGAGCCGGAAGCATAAAGTGTAGCTAGATCCGAAGGATGAGCCGGGCTGAATGATCGACCGAGACAGGCCCTGCGGGGCTGCACACGCGCCCCCACCCTTCGGGTAGGGGGAAAGGCCGCTAAAGCGGCTAAAAGCGCTCCAGCGTATTTCTGCGGGGTTTGGTGTGGGGTTTAGCGGGCTTTGCCCGCCTTTCCCCCTGCCGCGCAGCGGTGGGGCGGTGTGTAGCCTAGCGCAGCGAATAGACCAGCTATCCGGCCTCTGGCCGGGCATATTGGGCAAGGGCAGCAGCGCCCCACAAGGGCGCTGATAACCGCGCCTAGTGGATTATTCTTAGATAATCATGGATGGATTTTTCCAACACCCCGCCAGCCCCCGCCCCTGCTGGGTTTGCAGGTTTGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTATTGCAGGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTAGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_46.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_46.10,,Synthetic Biology,,,,2071,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Spectinomycin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCTAGAAATATTTTATCTGATTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_47.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_47.10,,Synthetic Biology,,,,2799,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Spectinomycin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_49.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_49.10,,Synthetic Biology,,,,2724,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Spectinomycin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGCAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_47_pKD46.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_47_pKD46.10,,Synthetic Biology,,,,2868,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Spectinomycin,,,,Low Copy,30 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGGGGTACAGTCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAAACATCATGAGGGAAGCGGTGATCGCCGAAGTATCGACTCAACTATCAGAGGTAGTTGGCGTCATCGAGCGCCATCTCGAACCGACGTTGCTGGCCGTACATTTGTACGGCTCCGCAGTGGATGGCGGCCTGAAGCCACACAGTGATATTGATTTGCTGGTTACGGTGACCGTAAGGCTTGATGAAACAACGCGGCGAGCTTTGATCAACGACCTTTTGGAAACTTCGGCTTCCCCTGGAGAGAGCGAGATTCTCCGCGCTGTAGAAGTCACCATTGTTGTGCACGACGACATCATTCCGTGGCGTTATCCAGCTAAGCGCGAACTGCAATTTGGAGAATGGCAGCGCAATGACATTCTTGCAGGTATCTTCGAGCCAGCCACGATCGACATTGATCTGGCTATCTTGCTGACAAAAGCAAGAGAACATAGCGTTGCCTTGGTAGGTCCAGCGGCGGAGGAACTCTTTGATCCGGTTCCTGAACAGGATCTATTTGAGGCGCTAAATGAAACCTTAACGCTATGGAACTCGCCGCCCGACTGGGCTGGCGATGAGCGAAATGTAGTGCTTACGTTGTCCCGCATTTGGTACAGCGCAGTAACCGGCAAAATCGCGCCGAAGGATGTCGCTGCCGACTGGGCAATGGAGCGCCTGCCGGCCCAGTATCAGCCCGTCATACTTGAAGCTAGACAGGCTTATCTTGGACAAGAAGAAGATCGCTTGGCCTCGCGCGCAGATCAGTTGGAAGAATTTGTCCACTACGTGAAAGGCGAGATCACCAAGGTAGTCGGCAAATAAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCTGTTCTACTTTTGTTTGTTAGTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTACGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGTTGCGGCCGCAATCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_5a5a.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_5a5a.10,,Synthetic Biology,,,,5291,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Tetracycline,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATTTGACAGCTTATCATCGATAAACTGTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATTCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCCTGCACGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGCCGTCCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACCATTGTGGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGAGCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGTAGGCCGGCCTCAGCCTGCCGCCTTGGGCCGGGTGATGTCGTACTTGCCCGCCGCGAACTCGGTTACCGTCCAGCCCAGCGCGACCAGCTCCGGCAACGCCTCGCGCACCCGCTGGCGGCGCTTGCGCATGGTCGAACCACTGGCCTCTGACGGCCAGACATAGCCGCACAAGGTATCTATGGAAGCCTTGCCGGTTTTGCCGGGGTCGATCCAGCCACACAGCCGCTGGTGCAGCAGGCGGGCGGTTTCGCTGTCCAGCGCCCGCACCTCGTCCATGCTGATGCGCACATGCTGGCCGCCACCCATGACGGCCTGCGCGATCAAGGGGTTCAGGGCCACGTACAGGCGCCCGTCCGCCTCGTCGCTGGCGTACTCCGACAGCAGCCGAAACCCCTGCCGCTTGCGGCCATTCTGGGCGATGATGGATACCTTCCAAAGGCGCTCGATGCAGTCCTGTATGTGCTTGAGCGCCCCACCACTATCGACCTCTGCCCCGATTTCCTTTGCCAGCGCCCGATAGCTACCTTTGACCACCATGGCATCAGCGGTGACGGCCTCCCACTTGGGTTCCAGGAACAGCCGGAGCTGCCGTCCGCCTTCGGTCTTGGGTTCCGGGCCAAGCACTAGGCCATTAGGCCCAGCCATGGCCACCAGCCCTTGCAGGATGCGCAGATCATCAGCGCCCAGCGGCTCCGGGCCGCTGAACTCGATCCGCTTGCCGTCGCCGTAGTCATACGTCACGTCCAGCTTGCTGCGCTTGCGCTCGCCCCGCTTGAGGGCACGGAACAGGCCGGGGGCCAGACAGTGCGCCGGGTCGTGCCGGACGTGGCTGAGGCTGTGCTTGTTCTTAGGCTTCACCACGGGGCACCCCCTTGCTCTTGCGCTGCCTCTCCAGCACGGCGGGCTTGAGCACCCCGCCGTCATGCCGCCTGAACCACCGATCAGCGAACGGTGCGCCATAGTTGGCCTTGCTCACACCGAAGCGGACGAAGAACCGGCGCTGGTCGTCGTCCACACCCCATTCCTCGGCCTCGGCGCTGGTCATGCTCGACAGGTAGGACTGCCAGCGGATGTTATCGACCAGTACCGAGCTGCCCCGGCTGGCCTGCTGCTGGTCGCCTGCGCCCATCATGGCCGCGCCCTTGCTGGCATGGTGCAGGAACACGATAGAGCACCCGGTATCGGCGGCGATGGCCTCCATGCGACCGATGACCTGGGCCATGGGGCCGCTGGCGTTTTCTTCCTCGATGTGGAACCGGCGCAGCGTGTCCAGCACCATCAGGCGGCGGCCCTCGGCGGCGCGCTTGAGGCCGTCGAACCACTCCGGGGCCATGATGTTGGGCAGGCTGCCGATCAGCGGCTGGATCAGCAGGCCGTCAGCCACGGCTTGCCGTTCCTCGGCGCTGAGGTGCGCCCCAAGGGCGTGCAGGCGGTGATGAATGGCGGTGGGCGGGTCTTCGGCGGGCAGGTAGATCACCGGGCCGGTGGGCAGTTCGCCCACCTCCAGCAGATCCGGCCCGCCTGCAATCTGTGCGGCCAGTTGCAGGGCCAGCATGGATTTACCGGCACCACCGGGCGACACCAGCGCCCCGACCGTACCGGCCACCATGTTGGGCAAAACGTAGTCCAGCGGTGGCGGCGCTGCTGCGAACGCCTCCAGAATATTGATAGGCTTATGGGTAGCCATTGATTGCCTCCTTTGCAGGCAGTTGGTGGTTAGGCGCTGGCGGGGTCACTACCCCCGCCCTGCGCCGCTCTGAGTTCTTCCAGGCACTCGCGCAGCGCCTCGTATTCGTCGTCGGTCAGCCAGAACTTGCGCTGACGCATCCCTTTGGCCTTCATGCGCTCGGCATATCGCGCTTGGCGTACAGCGTCAGGGCTGGCCAGCAGGTCGCCGGTCTGCTTGTCCTTTTGGTCTTTCATATCAGTCACCGAGAAACTTGCCGGGGCCGAAAGGCTTGTCTTCGCGGAACAAGGACAAGGTGCAGCCGTCAAGGTTAAGGCTGGCCATATCAGCGACTGAAAAGCGGCCAGCCTCGGCCTTGTTTGACGTATAACCAAAGCCACCGGGCAACCAATAGCCCTTGTCACTTTTGATCAGGTAGACCGACCCTGAAGCGCTTTTTTCGTATTCCATAAAACCCCCTTCTGTGCGTGAGTACTCATAGTATAACAGGCGTGAGTACCAACGCAAGCACTACATGCTGAAATCTGGCCCGCCCCTGTCCATGCCTCGCTGGCGGGGTGCCGGTGCCCGTGCCAGCTCGGCCCGCGCAAGCTGGACGCTGGGCAGACCCATGACCTTGCTGACGGTGCGCTCGATGTAATCCGCTTCGTGGCCGGGCTTGCGCTCTGCCAGCGCTGGGCTGGCCTCGGCCATGGCCTTGCCGATTTCCTCGGCACTGCGGCCCCGGCTGGCCAGCTTCTGCGCGGCGATAAAGTCGCACTTGCTGAGGTCATCACCGAAGCGCTTGACCAGCCCGGCCATCTCGCTGCGGTACTCGTCCAGCGCCGTGCGCCGGTGGCGGCTAAGCTGCCGCTCGGGCAGTTCGAGGCTGGCCAGCCTGCGGGCCTTCTCCTGCTGCCGCTGGGCCTGCTCGATCTGCTGGCCAGCCTGCTGCACCAGCGCCGGGCCAGCGGTGGCGGTCTTGCCCTTGGATTCACGCAGCAGCACCCACGGCTGATAACCGGCGCGGGTGGTGTGCTTGTCCTTGCGGTTGGTGAAGCCCGCCAAGCGGCCATAGTGGCGGCTGTCGGCGCTGGCCGGGTCGGCGTCGTACTCGCTGGCCAGCGTCCGGGCAATCTGCCCCCGAAGTTCACCGCCTGCGGCGTCGGCCACCTTGACCCATGCCTGATAGTTCTTCGGGCTGGTTTCCACTACCAGGGCAGGCTCCCGGCCCTCGGCTTTCATGTCATCCAGGTCAAACTCGCTGAGGTCGTCCACCAGCACCAGACCATGCCGCTCCTGCTCGGCGGGCCTGATATACACGTCATTGCCCTGGGCATTCATCCGCTTGAGCCATGGCGTGTTCTGGAGCACTTCGGCGGCTGACCATTCCCGGTTCATCATCTGGCCGGTGGTGGCGTCCCTGACGCCGATATCGAAGCGCTCACAGCCCATGGCCTTGAGCTGTCGGCCTATGGCCTGCAAAGTCCTGTCGTTCTTCATCGGGCCACCAAGCGATTCCCACACATTATACGAGCCGGAAGCATAAAGTGTAGCTAGATCCGAAGGATGAGCCGGGCTGAATGATCGACCGAGACAGGCCCTGCGGGGCTGCACACGCGCCCCCACCCTTCGGGTAGGGGGAAAGGCCGCTAAAGCGGCTAAAAGCGCTCCAGCGTATTTCTGCGGGGTTTGGTGTGGGGTTTAGCGGGCTTTGCCCGCCTTTCCCCCTGCCGCGCAGCGGTGGGGCGGTGTGTAGCCTAGCGCAGCGAATAGACCAGCTATCCGGCCTCTGGCCGGGCATATTGGGCAAGGGCAGCAGCGCCCCACAAGGGCGCTGATAACCGCGCCTAGTGGATTATTCTTAGATAATCATGGATGGATTTTTCCAACACCCCGCCAGCCCCCGCCCCTGCTGGGTTTGCAGGTTTGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTATTGCAGGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTAGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_5a6.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_5a6.10,,Synthetic Biology,,,,2349,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Tetracycline,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATTTGACAGCTTATCATCGATAAACTGTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATTCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCCTGCACGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGCCGTCCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACCATTGTGGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGAGCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCTAGAAATATTTTATCTGATTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_5a7.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_5a7.10,,Synthetic Biology,,,,3077,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Tetracycline,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATTTGACAGCTTATCATCGATAAACTGTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATTCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCCTGCACGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGCCGTCCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACCATTGTGGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGAGCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_5a9.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_5a9.10,,Synthetic Biology,,,,3002,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Tetracycline,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATTTGACAGCTTATCATCGATAAACTGTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATTCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCCTGCACGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGCCGTCCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACCATTGTGGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGAGCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGCAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_5a7_pKD46.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_5a7_pKD46.10,,Synthetic Biology,,,,3146,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Tetracycline,,,,Low Copy,30 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTACGTATTTAAATTTGACAGCTTATCATCGATAAACTGTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCGTCCATTCCGACAGCATCGCCAGTCACTATGGCGTGCTGCTAGCGCTATATGCGTTGATGCAATTTCTATGCGCACCCGTTCTCGGAGCACTGTCCGACCGCTTTGGCCGCCGCCCAGTCCTGCTCGCTTCGCTACTTGGAGCCACTATCGACTACGCGATCATGGCGACCACACCCGTCCTGTGGATTCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCCTGCACGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGCCGTCCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACCATTGTGGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGAGCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAGACAATTGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCTGTTCTACTTTTGTTTGTTAGTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTACGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGTTGCGGCCGCAATCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_65a.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_65a.10,,Synthetic Biology,,,,4821,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Gentamicin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGACATAAGCCTGTTCGGTTCGTAAACTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGTACAGCCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGAGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAACAATTCGTTCAAGCCGAGATCGGCTTCCCGGCCGCGGAGTTGTTCGGTAAATTGGACAACGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGTAGGCCGGCCTCAGCCTGCCGCCTTGGGCCGGGTGATGTCGTACTTGCCCGCCGCGAACTCGGTTACCGTCCAGCCCAGCGCGACCAGCTCCGGCAACGCCTCGCGCACCCGCTGGCGGCGCTTGCGCATGGTCGAACCACTGGCCTCTGACGGCCAGACATAGCCGCACAAGGTATCTATGGAAGCCTTGCCGGTTTTGCCGGGGTCGATCCAGCCACACAGCCGCTGGTGCAGCAGGCGGGCGGTTTCGCTGTCCAGCGCCCGCACCTCGTCCATGCTGATGCGCACATGCTGGCCGCCACCCATGACGGCCTGCGCGATCAAGGGGTTCAGGGCCACGTACAGGCGCCCGTCCGCCTCGTCGCTGGCGTACTCCGACAGCAGCCGAAACCCCTGCCGCTTGCGGCCATTCTGGGCGATGATGGATACCTTCCAAAGGCGCTCGATGCAGTCCTGTATGTGCTTGAGCGCCCCACCACTATCGACCTCTGCCCCGATTTCCTTTGCCAGCGCCCGATAGCTACCTTTGACCACCATGGCATCAGCGGTGACGGCCTCCCACTTGGGTTCCAGGAACAGCCGGAGCTGCCGTCCGCCTTCGGTCTTGGGTTCCGGGCCAAGCACTAGGCCATTAGGCCCAGCCATGGCCACCAGCCCTTGCAGGATGCGCAGATCATCAGCGCCCAGCGGCTCCGGGCCGCTGAACTCGATCCGCTTGCCGTCGCCGTAGTCATACGTCACGTCCAGCTTGCTGCGCTTGCGCTCGCCCCGCTTGAGGGCACGGAACAGGCCGGGGGCCAGACAGTGCGCCGGGTCGTGCCGGACGTGGCTGAGGCTGTGCTTGTTCTTAGGCTTCACCACGGGGCACCCCCTTGCTCTTGCGCTGCCTCTCCAGCACGGCGGGCTTGAGCACCCCGCCGTCATGCCGCCTGAACCACCGATCAGCGAACGGTGCGCCATAGTTGGCCTTGCTCACACCGAAGCGGACGAAGAACCGGCGCTGGTCGTCGTCCACACCCCATTCCTCGGCCTCGGCGCTGGTCATGCTCGACAGGTAGGACTGCCAGCGGATGTTATCGACCAGTACCGAGCTGCCCCGGCTGGCCTGCTGCTGGTCGCCTGCGCCCATCATGGCCGCGCCCTTGCTGGCATGGTGCAGGAACACGATAGAGCACCCGGTATCGGCGGCGATGGCCTCCATGCGACCGATGACCTGGGCCATGGGGCCGCTGGCGTTTTCTTCCTCGATGTGGAACCGGCGCAGCGTGTCCAGCACCATCAGGCGGCGGCCCTCGGCGGCGCGCTTGAGGCCGTCGAACCACTCCGGGGCCATGATGTTGGGCAGGCTGCCGATCAGCGGCTGGATCAGCAGGCCGTCAGCCACGGCTTGCCGTTCCTCGGCGCTGAGGTGCGCCCCAAGGGCGTGCAGGCGGTGATGAATGGCGGTGGGCGGGTCTTCGGCGGGCAGGTAGATCACCGGGCCGGTGGGCAGTTCGCCCACCTCCAGCAGATCCGGCCCGCCTGCAATCTGTGCGGCCAGTTGCAGGGCCAGCATGGATTTACCGGCACCACCGGGCGACACCAGCGCCCCGACCGTACCGGCCACCATGTTGGGCAAAACGTAGTCCAGCGGTGGCGGCGCTGCTGCGAACGCCTCCAGAATATTGATAGGCTTATGGGTAGCCATTGATTGCCTCCTTTGCAGGCAGTTGGTGGTTAGGCGCTGGCGGGGTCACTACCCCCGCCCTGCGCCGCTCTGAGTTCTTCCAGGCACTCGCGCAGCGCCTCGTATTCGTCGTCGGTCAGCCAGAACTTGCGCTGACGCATCCCTTTGGCCTTCATGCGCTCGGCATATCGCGCTTGGCGTACAGCGTCAGGGCTGGCCAGCAGGTCGCCGGTCTGCTTGTCCTTTTGGTCTTTCATATCAGTCACCGAGAAACTTGCCGGGGCCGAAAGGCTTGTCTTCGCGGAACAAGGACAAGGTGCAGCCGTCAAGGTTAAGGCTGGCCATATCAGCGACTGAAAAGCGGCCAGCCTCGGCCTTGTTTGACGTATAACCAAAGCCACCGGGCAACCAATAGCCCTTGTCACTTTTGATCAGGTAGACCGACCCTGAAGCGCTTTTTTCGTATTCCATAAAACCCCCTTCTGTGCGTGAGTACTCATAGTATAACAGGCGTGAGTACCAACGCAAGCACTACATGCTGAAATCTGGCCCGCCCCTGTCCATGCCTCGCTGGCGGGGTGCCGGTGCCCGTGCCAGCTCGGCCCGCGCAAGCTGGACGCTGGGCAGACCCATGACCTTGCTGACGGTGCGCTCGATGTAATCCGCTTCGTGGCCGGGCTTGCGCTCTGCCAGCGCTGGGCTGGCCTCGGCCATGGCCTTGCCGATTTCCTCGGCACTGCGGCCCCGGCTGGCCAGCTTCTGCGCGGCGATAAAGTCGCACTTGCTGAGGTCATCACCGAAGCGCTTGACCAGCCCGGCCATCTCGCTGCGGTACTCGTCCAGCGCCGTGCGCCGGTGGCGGCTAAGCTGCCGCTCGGGCAGTTCGAGGCTGGCCAGCCTGCGGGCCTTCTCCTGCTGCCGCTGGGCCTGCTCGATCTGCTGGCCAGCCTGCTGCACCAGCGCCGGGCCAGCGGTGGCGGTCTTGCCCTTGGATTCACGCAGCAGCACCCACGGCTGATAACCGGCGCGGGTGGTGTGCTTGTCCTTGCGGTTGGTGAAGCCCGCCAAGCGGCCATAGTGGCGGCTGTCGGCGCTGGCCGGGTCGGCGTCGTACTCGCTGGCCAGCGTCCGGGCAATCTGCCCCCGAAGTTCACCGCCTGCGGCGTCGGCCACCTTGACCCATGCCTGATAGTTCTTCGGGCTGGTTTCCACTACCAGGGCAGGCTCCCGGCCCTCGGCTTTCATGTCATCCAGGTCAAACTCGCTGAGGTCGTCCACCAGCACCAGACCATGCCGCTCCTGCTCGGCGGGCCTGATATACACGTCATTGCCCTGGGCATTCATCCGCTTGAGCCATGGCGTGTTCTGGAGCACTTCGGCGGCTGACCATTCCCGGTTCATCATCTGGCCGGTGGTGGCGTCCCTGACGCCGATATCGAAGCGCTCACAGCCCATGGCCTTGAGCTGTCGGCCTATGGCCTGCAAAGTCCTGTCGTTCTTCATCGGGCCACCAAGCGATTCCCACACATTATACGAGCCGGAAGCATAAAGTGTAGCTAGATCCGAAGGATGAGCCGGGCTGAATGATCGACCGAGACAGGCCCTGCGGGGCTGCACACGCGCCCCCACCCTTCGGGTAGGGGGAAAGGCCGCTAAAGCGGCTAAAAGCGCTCCAGCGTATTTCTGCGGGGTTTGGTGTGGGGTTTAGCGGGCTTTGCCCGCCTTTCCCCCTGCCGCGCAGCGGTGGGGCGGTGTGTAGCCTAGCGCAGCGAATAGACCAGCTATCCGGCCTCTGGCCGGGCATATTGGGCAAGGGCAGCAGCGCCCCACAAGGGCGCTGATAACCGCGCCTAGTGGATTATTCTTAGATAATCATGGATGGATTTTTCCAACACCCCGCCAGCCCCCGCCCCTGCTGGGTTTGCAGGTTTGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTATTGCAGGGGGGCGTGACAGTTATTGCAGGGGTTCGTGACAGTTAGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_66.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_66.10,,Synthetic Biology,,,,1879,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Gentamicin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGACATAAGCCTGTTCGGTTCGTAAACTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGTACAGCCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGAGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAACAATTCGTTCAAGCCGAGATCGGCTTCCCGGCCGCGGAGTTGTTCGGTAAATTGGACAACGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCTAGAAATATTTTATCTGATTAATAAGATGATCTTCTTGAGATCGTTTTGGTCTGCGCGTAATCTCTTGCTCTGAAAACGAAAAAACCGCCTTGCAGGGCGGTTTTTCGAAGGTTCTCTGAGCTACCAACTCTTTGAACCGAGGTAACTGGCTTGGAGGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGTGGTGCTTTTGCATGTCTTTCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGACTGAACGGGGGGTTCGTGCATACAGTCCAGCTTGGAGCGAACTGCCTACCCGGAACTGAGTGTCAGGCGTGGAATGAGACAAACGCGGCCATAACAGCGGAATGACACCGGTAAACCGAAAGGCAGGAACAGGAGAGCGCACGAGGGAGCCGCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGATTTGAGCGTCAGATTTCGTGATGCTTGTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTTCGTAAGCCATTTCCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAATATATCCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_67.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_67.10,,Synthetic Biology,,,,2607,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Gentamicin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGACATAAGCCTGTTCGGTTCGTAAACTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGTACAGCCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGAGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAACAATTCGTTCAAGCCGAGATCGGCTTCCCGGCCGCGGAGTTGTTCGGTAAATTGGACAACGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTATGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACATGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_69.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,878,Synthetic,,,Not applicable,,,BASIC_SEVA_69.10,,Synthetic Biology,,,,2532,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Gentamicin,,,,Low Copy,37 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGACATAAGCCTGTTCGGTTCGTAAACTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGTACAGCCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGAGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAACAATTCGTTCAAGCCGAGATCGGCTTCCCGGCCGCGGAGTTGTTCGGTAAATTGGACAACGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCGGCCGGCCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGCAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCTTTACGGCTAGCTCAGTCCTAGGTATAGTGCTAGCTACTAGTGAAAGAGGAGAAATACTAGTATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, +BASIC_SEVA_67_pKD46.10,Encodes one insert,BASIC DNA assembly vector,,mScarlet counter-selection cassette,,,,,1074,Synthetic,,,Not applicable,,,BASIC_SEVA_67_pKD46.10,,Synthetic Biology,,,,2676,Unknown,,,,,,ggcggcggatttgtcctac,ggtgagaatccaggggtcc,Gentamicin,,,,Low Copy,30 C,DH5alpha,,No,No,,,Cloning Method: BASIC DNA assembly,GGCTCGGGTAAGAACTCGCACTTCGTGGAAACACTATTATCTGGTGGGTCTCTGTCCACTAGTCTTGGACTCCTGTTGATAGATCCAGTAATGACCTCAGAACTCCATCTGGATTTGTTCAGAACGCTCGGTTGCCGCCGGGCGTTTTTTATTGGTGAGAATCCAGGGGTCCCCAATAATTACGATTTAAATTTGACATAAGCCTGTTCGGTTCGTAAACTGTAATGCAAGTAGCGTATGCGCTCACGCAACTGGTCCAGAACCTTGACCGAACGCAGCGGTGGTAACGGCGCAGTGGCGGTTTTCATGGCTTGTTATGACTGTTTTTTTGTACAGCCTATGCCTCGGGCATCCAAGCAGCAAGCGCGTTACGCCGTGGGTCGATGTTTGATGTTATGGAGCAGCAACGATGTTACGCAGCAGCAACGATGTTACGCAGCAGGGCAGTCGCCCTAAAACAAAGTTAGGTGGCTCAAGTATGGGCATCATTCGCACATGTAGGCTCGGCCCTGACCAAGTCAAATCCATGCGGGCTGCTCTTGATCTTTTCGGTCGTGAGTTCGGAGACGTAGCCACCTACTCCCAACATCAGCCGGACTCCGATTACCTCGGGAACTTGCTCCGTAGTAAGACATTCATCGCGCTTGCTGCCTTCGACCAAGAAGCGGTTGTTGGCGCTCTCGCGGCTTACGTTCTGCCCAAGTTTGAGCAGCCGCGTAGTGAGATCTATATCTATGATCTCGCAGTCTCCGGAGAGCACCGGAGGCAGGGCATTGCCACCGCGCTCATCAATCTCCTCAAGCATGAGGCCAACGCGCTTGGTGCTTATGTGATCTACGTGCAAGCAGATTACGGTGACGATCCCGCAGTGGCTCTCTATACAAAGTTGGGCATACGGGAAGAAGTGATGCACTTTGATATCGACCCAAGTACCGCCACCTAACAATTCGTTCAAGCCGAGATCGGCTTCCCGGCCGCGGAGTTGTTCGGTAAATTGGACAACGGTCGGCTCGGCCCACTTGTGTGTCTCGTACAATTCTGAGGTCCACACAGATAGTGTCCTGTTCTACTTTTGTTTGTTAGTCTTGATGCTTCACTGATAGATACAAGAGCCATAAGAACCTCAGATCCTTCCGTATTTAGCCAGTATGTTCTCTAGTGTGGTTCGTTGTTTTTGCGTGAGCCATGAGAACGAACCATTGAGATCATACTTACTTTGCATGTCACTCAAAAATTTTGCCTCAAAACTGGTGAGCTGAATTTTTGCAGTTAAAGCATCGTGTAGTGTTTTTCTTAGTCCGTTACGTAGGTAGGAATCTGATGTAATGGTTGTTGGTATTTTGTCACCATTCATTTTTATCTGGTTGTTCTCAAGTTCGGTTACGAGATCCATTTGTCTATCTAGTTCAACTTGGAAAATCAACGTATCAGTCGGGCGGCCTCGCTTATCAACCACCAATTTCATATTGCTGTAAGTGTTTAAATCTTTACTTATTGGTTTCAAAACCCATTGGTTAAGCCTTTTAAACTCATGGTAGTTATTTTCAAGCATTAACATGAACTTAAATTCATCAAGGCTAATCTCTATATTTGCCTTGTGAGTTTTCTTTTGTGTTAGTTCTTTTAATAACCACTCATAAATCCTCATAGAGTATTTGTTTTCAAAAGACTTAACATGTTCCAGATTATATTTTATGAATTTTTTTAACTGGAAAAGATAAGGCAATATCTCTTCACTAAAAACTAATTCTAATTTTTCGCTTGAGAACTTGGCATAGTTTGTCCACTGGAAAATCTCAAAGCCTTTAACCAAAGGATTCCTGATTTCCACAGTTCTCGTCATCAGCTCTCTGGTTGCTTTAGCTAATACACCATAAGCATTTTCCCTACTGATGTTCATCATCTGAGCGTATTGGTTATAAGTGAACGATACCGTCCGTTCTTTCCTTGTAGGGTTTTCAATCGTGGGGTTGAGTAGTGCCACACAGCATAAAATTAGCTTGGTTTCATGCTCCGTTAAGTCATAGCGACTAATCGCTAGTTCATTTGCTTTGAAAACAACTAATTCAGACATACATCTCAATTGGTCTAGGTGATTTTAATCACTATACCAATTGAGATGGGCTAGTCAATGATAATTACTAGTCCTTTTCCTTTGAGTTGTGGGTATCTGTAAATTCTGCTAGACCTTTGCTGGAAAACTTGTAAATTCTGCTAGACCCTCTGTAAATTCCGCTAGACCTTTGTGTGTTTTTTTTGTTTATATTCAAGTGGTTATAATTTATAGAATAAAGAAAGAATAAAAAAAGATAAAAAGAATAGATCCCAGCCCTGTGTATAACTCACTACTTTAGTCAGTTCCGCAGTATTACAAAAGGATGTCGCAAACGCTGTTTGCTCCTCTACAAAACAGACCTTAAAACCCTAAAGGCTTAAGTAGCACCCTCGCAAGCTCGGTTGCGGCCGCAATCGGGCAAATCGCTGAATATTCCTTTTGTCTCCGACCATCAGGCACCTGAGTCGCTGTCTTTTTCGTGACATTCAGTTCGCTGCGCTCACGGCTCTGGCAGTGAATGGGGGTAAATGGCACTACAGGCGCGGCGCGCCCAGCTGTCTAGGGCGGCGGATTTGTCCTACTCAGGAGAGCGTTCACCGACAAACAACAGATAAAACGAAAGGCCCAGTCTTTCGACTGAGCCTTTCGTTTTATTTGATGCCTTTAATTAAGGCTCGGGAGACCTATCGGTAATAACAGTCCAATCTGGTGTAACTTCGGAATCGTCCCCAATTATTGAACACCCTTCGGGGTGTTTTTTTGTTTCTGGTCTACCATCTCGTTGTGATAATAGACCTGAAGTGCCTACTCTGGAAAATCTTTGACAGCTAGCTCAGTCCTAGGTATAATGCTAGCAGCTGTCACCGGATGTGCTTTCCGGTCTGATGAGTCCGTGAGGACGAAACAGCCTCTACAAATAATTTTGTTTAAGGCTCGCTACGAGAAGATTGTTACTTTCGCAGCGTTATTATCTAAGGAGGTAGTCCATGGTTAGCAAAGGCGAGGCGGTTATCAAGGAGTTTATGCGTTTTAAGGTTCACATGGAGGGTAGCATGAATGGTCACGAGTTCGAGATCGAGGGTGAAGGCGAGGGTCGTCCGTACGAAGGCACCCAGACCGCGAAGCTGAAAGTGACCAAGGGTGGCCCGCTGCCGTTCAGCTGGGACATCCTGAGCCCGCAGTTCATGTATGGCAGCCGTGCGTTTACCAAACACCCGGCGGACATTCCGGATTACTATAAGCAAAGCTTCCCGGAAGGTTTTAAATGGGAGCGTGTTATGAACTTCGAAGATGGTGGCGCGGTGACCGTTACCCAGGACACCAGCCTGGAGGATGGCACCCTGATTTACAAGGTGAAACTGCGTGGCACCAACTTTCCGCCGGATGGTCCGGTTATGCAGAAGAAAACGATGGGTTGGGAAGCGAGCACCGAGCGTCTGTATCCGGAAGATGGCGTGCTGAAGGGTGATATCAAAATGGCGCTGCGTCTGAAGGACGGTGGCCGTTACCTGGCGGATTTTAAGACCACCTATAAAGCGAAGAAACCGGTGCAAATGCCGGGTGCGTACAACGTTGACCGTAAACTGGATATTACCAGCCACAACGAGGATTATACCGTGGTTGAGCAATATGAGCGTAGCGAGGGTCGCCACAGCACCGGCGGCATGGACGAACTGTATAAGGGATCCTAATAA,,,, diff --git a/csv_xlsx_files/BASIC_SEVA_10_bsaI_digest.csv b/csv_xlsx_files/BASIC_SEVA_10_bsaI_digest.csv new file mode 100644 index 0000000..bb64957 --- /dev/null +++ b/csv_xlsx_files/BASIC_SEVA_10_bsaI_digest.csv @@ -0,0 +1,31 @@ +Assembly ID,Fragment 1 Length (bp),Fragment 2 Length (bp) +BASIC_SEVA_15a.10,878,5055 +BASIC_SEVA_16.10,878,2113 +BASIC_SEVA_17.10,1074,2841 +BASIC_SEVA_19.10,878,2766 +BASIC_SEVA_17_pKD46.10,1074,2910 +BASIC_SEVA_25a.10,878,4943 +BASIC_SEVA_26.10,878,2001 +BASIC_SEVA_27.10,1074,2729 +BASIC_SEVA_29.10,878,2654 +BASIC_SEVA_27_pKD46.10,1074,2798 +BASIC_SEVA_35a.10,878,4799 +BASIC_SEVA_36.10,878,1857 +BASIC_SEVA_37.10,1074,2585 +BASIC_SEVA_39.10,878,2510 +BASIC_SEVA_37_pKD46.10,1074,2654 +BASIC_SEVA_45a.10,878,5013 +BASIC_SEVA_46.10,878,2071 +BASIC_SEVA_47.10,1074,2799 +BASIC_SEVA_49.10,878,2724 +BASIC_SEVA_47_pKD46.10,1074,2868 +BASIC_SEVA_5a5a.10,878,5291 +BASIC_SEVA_5a6.10,878,2349 +BASIC_SEVA_5a7.10,1074,3077 +BASIC_SEVA_5a9.10,878,3002 +BASIC_SEVA_5a7_pKD46.10,1074,3146 +BASIC_SEVA_65a.10,878,4821 +BASIC_SEVA_66.10,878,1879 +BASIC_SEVA_67.10,1074,2607 +BASIC_SEVA_69.10,878,2532 +BASIC_SEVA_67_pKD46.10,1074,2676 diff --git a/csv_xlsx_files/BASIC_SEVA_10_overnights.csv b/csv_xlsx_files/BASIC_SEVA_10_overnights.csv new file mode 100644 index 0000000..c201dd7 --- /dev/null +++ b/csv_xlsx_files/BASIC_SEVA_10_overnights.csv @@ -0,0 +1,97 @@ +Overnight Well,Transformation Well,Marker,Name +A1,A1,Ampicillin,BASIC_SEVA_15a.10 +B1,A1,Ampicillin,BASIC_SEVA_15a.10 +C1,A1,Ampicillin,BASIC_SEVA_15a.10 +D1,A1,Ampicillin,BASIC_SEVA_15a.10 +E1,B1,Ampicillin,BASIC_SEVA_16.10 +F1,B1,Ampicillin,BASIC_SEVA_16.10 +G1,B1,Ampicillin,BASIC_SEVA_16.10 +H1,B1,Ampicillin,BASIC_SEVA_16.10 +A2,C1,Ampicillin,BASIC_SEVA_17.10 +B2,C1,Ampicillin,BASIC_SEVA_17.10 +C2,C1,Ampicillin,BASIC_SEVA_17.10 +D2,C1,Ampicillin,BASIC_SEVA_17.10 +E2,D1,Ampicillin,BASIC_SEVA_19.10 +F2,D1,Ampicillin,BASIC_SEVA_19.10 +G2,D1,Ampicillin,BASIC_SEVA_19.10 +H2,D1,Ampicillin,BASIC_SEVA_19.10 +A3,F1,Kanamycin,BASIC_SEVA_25a.10 +B3,F1,Kanamycin,BASIC_SEVA_25a.10 +C3,F1,Kanamycin,BASIC_SEVA_25a.10 +D3,F1,Kanamycin,BASIC_SEVA_25a.10 +E3,G1,Kanamycin,BASIC_SEVA_26.10 +F3,G1,Kanamycin,BASIC_SEVA_26.10 +G3,G1,Kanamycin,BASIC_SEVA_26.10 +H3,G1,Kanamycin,BASIC_SEVA_26.10 +A4,H1,Kanamycin,BASIC_SEVA_27.10 +B4,H1,Kanamycin,BASIC_SEVA_27.10 +C4,H1,Kanamycin,BASIC_SEVA_27.10 +D4,H1,Kanamycin,BASIC_SEVA_27.10 +E4,A2,Kanamycin,BASIC_SEVA_29.10 +F4,A2,Kanamycin,BASIC_SEVA_29.10 +G4,A2,Kanamycin,BASIC_SEVA_29.10 +H4,A2,Kanamycin,BASIC_SEVA_29.10 +A5,C2,Chloramphenicol,BASIC_SEVA_35a.10 +B5,C2,Chloramphenicol,BASIC_SEVA_35a.10 +C5,C2,Chloramphenicol,BASIC_SEVA_35a.10 +D5,C2,Chloramphenicol,BASIC_SEVA_35a.10 +E5,D2,Chloramphenicol,BASIC_SEVA_36.10 +F5,D2,Chloramphenicol,BASIC_SEVA_36.10 +G5,D2,Chloramphenicol,BASIC_SEVA_36.10 +H5,D2,Chloramphenicol,BASIC_SEVA_36.10 +A6,E2,Chloramphenicol,BASIC_SEVA_37.10 +B6,E2,Chloramphenicol,BASIC_SEVA_37.10 +C6,E2,Chloramphenicol,BASIC_SEVA_37.10 +D6,E2,Chloramphenicol,BASIC_SEVA_37.10 +E6,F2,Chloramphenicol,BASIC_SEVA_39.10 +F6,F2,Chloramphenicol,BASIC_SEVA_39.10 +G6,F2,Chloramphenicol,BASIC_SEVA_39.10 +H6,F2,Chloramphenicol,BASIC_SEVA_39.10 +A7,H2,Streptomycin/Spectinomycin,BASIC_SEVA_45a.10 +B7,H2,Streptomycin/Spectinomycin,BASIC_SEVA_45a.10 +C7,H2,Streptomycin/Spectinomycin,BASIC_SEVA_45a.10 +D7,H2,Streptomycin/Spectinomycin,BASIC_SEVA_45a.10 +E7,A3,Streptomycin/Spectinomycin,BASIC_SEVA_46.10 +F7,A3,Streptomycin/Spectinomycin,BASIC_SEVA_46.10 +G7,A3,Streptomycin/Spectinomycin,BASIC_SEVA_46.10 +H7,A3,Streptomycin/Spectinomycin,BASIC_SEVA_46.10 +A8,B3,Streptomycin/Spectinomycin,BASIC_SEVA_47.10 +B8,B3,Streptomycin/Spectinomycin,BASIC_SEVA_47.10 +C8,B3,Streptomycin/Spectinomycin,BASIC_SEVA_47.10 +D8,B3,Streptomycin/Spectinomycin,BASIC_SEVA_47.10 +E8,C3,Streptomycin/Spectinomycin,BASIC_SEVA_49.10 +F8,C3,Streptomycin/Spectinomycin,BASIC_SEVA_49.10 +G8,C3,Streptomycin/Spectinomycin,BASIC_SEVA_49.10 +H8,C3,Streptomycin/Spectinomycin,BASIC_SEVA_49.10 +A9,E3,Tetracycline,BASIC_SEVA_5a5a.10 +B9,E3,Tetracycline,BASIC_SEVA_5a5a.10 +C9,E3,Tetracycline,BASIC_SEVA_5a5a.10 +D9,E3,Tetracycline,BASIC_SEVA_5a5a.10 +E9,F3,Tetracycline,BASIC_SEVA_5a6.10 +F9,F3,Tetracycline,BASIC_SEVA_5a6.10 +G9,F3,Tetracycline,BASIC_SEVA_5a6.10 +H9,F3,Tetracycline,BASIC_SEVA_5a6.10 +A10,G3,Tetracycline,BASIC_SEVA_5a7.10 +B10,G3,Tetracycline,BASIC_SEVA_5a7.10 +C10,G3,Tetracycline,BASIC_SEVA_5a7.10 +D10,G3,Tetracycline,BASIC_SEVA_5a7.10 +E10,H3,Tetracycline,BASIC_SEVA_5a9.10 +F10,H3,Tetracycline,BASIC_SEVA_5a9.10 +G10,H3,Tetracycline,BASIC_SEVA_5a9.10 +H10,H3,Tetracycline,BASIC_SEVA_5a9.10 +A11,B4,Gentamicin,BASIC_SEVA_65a.10 +B11,B4,Gentamicin,BASIC_SEVA_65a.10 +C11,B4,Gentamicin,BASIC_SEVA_65a.10 +D11,B4,Gentamicin,BASIC_SEVA_65a.10 +E11,C4,Gentamicin,BASIC_SEVA_66.10 +F11,C4,Gentamicin,BASIC_SEVA_66.10 +G11,C4,Gentamicin,BASIC_SEVA_66.10 +H11,C4,Gentamicin,BASIC_SEVA_66.10 +A12,D4,Gentamicin,BASIC_SEVA_67.10 +B12,D4,Gentamicin,BASIC_SEVA_67.10 +C12,D4,Gentamicin,BASIC_SEVA_67.10 +D12,D4,Gentamicin,BASIC_SEVA_67.10 +E12,E4,Gentamicin,BASIC_SEVA_69.10 +F12,E4,Gentamicin,BASIC_SEVA_69.10 +G12,E4,Gentamicin,BASIC_SEVA_69.10 +H12,E4,Gentamicin,BASIC_SEVA_69.10 diff --git a/csv_xlsx_files/BASIC_SEVA_benchling_misc_features_library.csv b/csv_xlsx_files/BASIC_SEVA_benchling_misc_features_library.csv index c3b21af..6ea3dfb 100644 --- a/csv_xlsx_files/BASIC_SEVA_benchling_misc_features_library.csv +++ b/csv_xlsx_files/BASIC_SEVA_benchling_misc_features_library.csv @@ -6,10 +6,10 @@ J23106,tttacggctagctcagtcctaggtatagtgctagc,regulatory,#FAAC61,nucleotide J23119,ttgacagctagctcagtcctaggtataatgctagc,regulatory,#FAAC61,nucleotide LMP,ggctcgggtaagaactcgcacttcgtggaaacactattatctggtgggtctctgtcc,misc_feature,#F58A5E,nucleotide LMS,ggctcgggagacctatcggtaataacagtccaatctggtgtaacttcggaatcgtcc,misc_feature,#F58A5E,nucleotide -mScarlett,atggttagcaaaggcgaggcggttatcaaggagtttatgcgttttaaggttcacatggagggtagcatgaatggtcacgagttcgagatcgagggtgaaggcgagggtcgtccgtacgaaggcacccagaccgcgaagctgaaagtgaccaagggtggcccgctgccgttcagctgggacatcctgagcccgcagttcatgtatggcagccgtgcgtttaccaaacacccggcggacattccggattactataagcaaagcttcccggaaggttttaaatgggagcgtgttatgaacttcgaagatggtggcgcggtgaccgttacccaggacaccagcctggaggatggcaccctgatttacaaggtgaaactgcgtggcaccaactttccgccggatggtccggttatgcagaagaaaacgatgggttgggaagcgagcaccgagcgtctgtatccggaagatggcgtgctgaagggtgatatcaaaatggcgctgcgtctgaaggacggtggccgttacctggcggattttaagaccacctataaagcgaagaaaccggtgcaaatgccgggtgcgtacaacgttgaccgtaaactggatattaccagccacaacgaggattataccgtggttgagcaatatgagcgtagcgagggtcgccacagcaccggcggcatggacgaactgtataagggatcctaa,CDS,#FFEF86,nucleotide +mScarlet,atggttagcaaaggcgaggcggttatcaaggagtttatgcgttttaaggttcacatggagggtagcatgaatggtcacgagttcgagatcgagggtgaaggcgagggtcgtccgtacgaaggcacccagaccgcgaagctgaaagtgaccaagggtggcccgctgccgttcagctgggacatcctgagcccgcagttcatgtatggcagccgtgcgtttaccaaacacccggcggacattccggattactataagcaaagcttcccggaaggttttaaatgggagcgtgttatgaacttcgaagatggtggcgcggtgaccgttacccaggacaccagcctggaggatggcaccctgatttacaaggtgaaactgcgtggcaccaactttccgccggatggtccggttatgcagaagaaaacgatgggttgggaagcgagcaccgagcgtctgtatccggaagatggcgtgctgaagggtgatatcaaaatggcgctgcgtctgaaggacggtggccgttacctggcggattttaagaccacctataaagcgaagaaaccggtgcaaatgccgggtgcgtacaacgttgaccgtaaactggatattaccagccacaacgaggattataccgtggttgagcaatatgagcgtagcgagggtcgccacagcaccggcggcatggacgaactgtataagggatcctaa,CDS,#FFEF86,nucleotide pSC101 ori,gggctagtcaatgataattactagtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactc,rep_origin,#FFEF86,nucleotide RBS-A12,taaggagg,regulatory,#F58A5E,nucleotide -Rep101(Ts),atgtctgaattagttgttttcaaagcaaatgaactagcgattagtcgctatgacttaacggagcatgaaaccaagctaattttatgctgtgtggcactactcaaccccacgattgaaaaccctacaaggaaagaacggacggtatcgttcacttataaccaatacgttcagatgatgaacatcagtagggaaaatgcttatggtgtattagctaaagcaaccagagagctgatgacgagaactgtggaaatcaggaatcctttggttaaaggctttgagattttccagtggacaaactatgccaagttctcaagcgaaaaattagaattagtttttagtgaagagatattgccttatcttttccagttaaaaaaattcataaaatataatctggaacatgttaagtcttttgaaaacaaatactctatgaggatttatgagtggttattaaaagaactaacacaaaagaaaactcacaaggcaaatatagagattagccttgatgaatttaagttcatgttaatgcttgaaaataactaccatgagtttaaaaggcttaaccaatgggttttgaaaccaataagtaaagatttaaacacttacagcaatatgaaattggtggttgataagcgaggccgcccgactgatacgttgattttccaagttgaactagatagacaaatggatctcgtaaccgaacttgagaacaaccagataaaaatgaatggtgacaaaataccaacaaccattacatcagattcctacctacataacggactaagaaaaacactacacgatgctttaactgcaaaaattcagctcaccagttttgaggcaaaatttttgagtgacatgcaaagtaagtatgatctcaatggttcgttctcatggctcacgcaaaaacaacgaaccacactagagaacatactggctaaatacggaaggatctga,CDS,#993366,nucleotide +Rep101(pKD46),tcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttacgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacat,CDS,#993366,nucleotide SEVA_Ap,tagtagcccgcctaatgagcgggcttttttttaattcccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagcattcagcattttcgtgtggcgctgattccgttttttgcggcgttttgcctgccggtgtttgcgcatccggaaaccctggtgaaagtgaaagatgcggaagatcaactgggtgcgcgcgtgggctatattgaactggatctgaacagcggcaaaattctggaatcttttcgtccggaagaacgttttccgatgatgagcacctttaaagtgctgctgtgcggtgcggttctgagccgtgtggatgcgggccaggaacaactgggccgtcgtattcattatagccagaacgatctggtggaatatagcccggtgaccgaaaaacatctgaccgatggcatgaccgtgcgtgaactgtgcagcgcggcgattaccatgagcgataacaccgcggcgaacctgctgctgacgaccattggcggtccgaaagaactgaccgcgtttctgcataacatgggcgatcatgtgacccgtctggatcgttgggaaccggaactgaacgaagcgattccgaacgatgaacgtgataccaccatgccggcagcaatggcgaccaccctgcgtaaactgctgacgggtgagctgctgaccctggcaagccgccagcaactgattgattggatggaagcggataaagtggcgggtccgctgctgcgtagcgcgctgccggctggctggtttattgcggataaaagcggtgcgggcgaacgtggcagccgtggcattattgcggcgctgggcccggatggtaaaccgagccgtattgtggtgatttataccaccggcagccaggcgacgatggatgaacgtaaccgtcagattgcggaaattggcgcgagcctgattaaacattggtaaaccgatacaattaaaggctccttttggagcctttttttttggac,misc_feature,#F58A5E,nucleotide SEVA_Cm,tggcgaaaatgagacgttgatcggcacgtaagaggttccaactttcaccataatgaaataagatcactaccgggcgtattttttgagttatcgagattttcaggagctaaggaagctaaaatggagaaaaaaatcactggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaatgctcatccggaatttcgtatggcaatgaaagacggtgagctggtgatatgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcatcgctctggagtgaataccacgacgatttccggcagtttctacacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgtttttcgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcaccatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgattcaggttcatcatgccgtttgtgatggcttccatgtcggcagaatgcttaatgaattacaacagtactgcgatgagtggcagggcggggcgtaattt,misc_feature,#F58A5E,nucleotide SEVA_Gm,ttgacataagcctgttcggttcgtaaactgtaatgcaagtagcgtatgcgctcacgcaactggtccagaaccttgaccgaacgcagcggtggtaacggcgcagtggcggttttcatggcttgttatgactgtttttttgtacagcctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagcagcaacgatgttacgcagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaggtggctcaagtatgggcatcattcgcacatgtaggctcggccctgaccaagtcaaatccatgcgggctgctcttgatcttttcggtcgtgagttcggagacgtagccacctactcccaacatcagccggactccgattacctcgggaacttgctccgtagtaagacattcatcgcgcttgctgccttcgaccaagaagcggttgttggcgctctcgcggcttacgttctgcccaagtttgagcagccgcgtagtgagatctatatctatgatctcgcagtctccggagagcaccggaggcagggcattgccaccgcgctcatcaatctcctcaagcatgaggccaacgcgcttggtgcttatgtgatctacgtgcaagcagattacggtgacgatcccgcagtggctctctatacaaagttgggcatacgggaagaagtgatgcactttgatatcgacccaagtaccgccacctaacaattcgttcaagccgagatcggcttcccggccgcggagttgttcggtaaattg,misc_feature,#F58A5E,nucleotide @@ -22,8 +22,8 @@ SEVA_pRO1600/ColE1,gataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccg SEVA_pSC101,tcagatccttccgtatttagccagtatgttctctagtgtggttcgttgtttttgcgtgagccatgagaacgaaccattgagatcatacttactttgcatgtcactcaaaaattttgcctcaaaactggtgagctgaatttttgcagttaaagcatcgtgtagtgtttttcttagtccgttatgtaggtaggaatctgatgtaatggttgttggtattttgtcaccattcatttttatctggttgttctcaagttcggttacgagatccatttgtctatctagttcaacttggaaaatcaacgtatcagtcgggcggcctcgcttatcaaccaccaatttcatattgctgtaagtgtttaaatctttacttattggtttcaaaacccattggttaagccttttaaactcatggtagttattttcaagcattaacatgaacttaaattcatcaaggctaatctctatatttgccttgtgagttttcttttgtgttagttcttttaataaccactcataaatcctcatagagtatttgttttcaaaagacttaacatgttccagattatattttatgaatttttttaactggaaaagataaggcaatatctcttcactaaaaactaattctaatttttcgcttgagaacttggcatagtttgtccactggaaaatctcaaagcctttaaccaaaggattcctgatttccacagttctcgtcatcagctctctggttgctttagctaatacaccataagcattttccctactgatgttcatcatctgagcgtattggttataagtgaacgataccgtccgttctttccttgtagggttttcaatcgtggggttgagtagtgccacacagcataaaattagcttggtttcatgctccgttaagtcatagcgactaatcgctagttcatttgctttgaaaacaactaattcagacatacatctcaattggtctaggtgattttaatcactataccaattgagatgggctagtcaatgataattacatgtccttttcctttgagttgtgggtatctgtaaattctgctagacctttgctggaaaacttgtaaattctgctagaccctctgtaaattccgctagacctttgtgtgttttttttgtttatattcaagtggttataatttatagaataaagaaagaataaaaaaagataaaaagaatagatcccagccctgtgtataactcactactttagtcagttccgcagtattacaaaaggatgtcgcaaacgctgtttgctcctctacaaaacagaccttaaaaccctaaaggcttaagtagcaccctcgcaagctcgggcaaatcgctgaatattccttttgtctccgaccatcaggcacctgagtcgctgtctttttcgtgacattcagttcgctgcgctcacggctctggcagtgaatgggggtaaatggcactacaggcgc,misc_feature,#F58A5E,nucleotide SEVA_pUC,ccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctttgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggca,misc_feature,#F58A5E,nucleotide SEVA_RK2,gcgatgcaggtggctgctgaacccccagccggaactgaccccacaaggccctagcgtttgcaatgcaccaggtcatcattgacccaggcgtgttccaccaggccgctgcctcgcaactcttcgcaggcttcgccgacctgctcgcgccacttcttcacgcgggtggaatccgatccgcacatgaggcggaaggtttccagcttgagcgggtacggctcccggtgcgagctgaaatagtcgaacatccgtcgggccgtcggcgacagcttgcggtacttctcccatatgaatttcgtgtagtggtcgccagcaaacagcacgacgatttcctcgtcgatcaggacctggcaacgggacgttttcttgccacggtccaggacgcggaagcggtgcagcagcgacaccgattccaggtgcccaacgcggtcggacgtgaagcccatcgccgtcgcctgtaggcgcgacaggcattcctcggccttcgtgtaataccggccattgatcgaccagcccaggtcctggcaaagctcgtagaacgtgaaggtgatcggctcgccgataggggtgcgcttcgcgtactccaacacctgctgccacaccagttcgtcatcgtcggcccgcagctcgacgccggtgtaggtgatcttcacgtccttgttgacgtggaaaatgaccttgttttgcagcgcctcgcgcgggattttcttgttgcgcgtggtgaacagggcagagcgggccgtgtcgtttggcatcgctcgcatcgtgtccggccacggcgcaatatcgaacaaggaaagctgcatttccttgatctgctgcttcgtgtgtttcagcaacgcggcctgcttggcttcgctgacctgttttgccaggtcctcgccggcggtttttcgcttcttggtcgtcatagttcctcgcgtgtcgatggtcatcgacttcgccaaacctgccgcctcctgttcgagacgacgcgaacgctccacggcggccgatggcgcgggcagggcagggggagccagttgcacgctgtcgcgctcgatcttggccgtagcttgctggactatcgagccgacggactggaaggtttcgcggggcgcacgcatgacggtgcggcttgcgatggtttcggcatcctcggcggaaaaccccgcgtcgatcagttcttgcctgtatgccttccggtcaaacgtccgattcattcaccctccttgcgggattgccccggaattaattccccggatcgatccgtcgatcttgatcccctgcgccatcagatccttggcggcaagaaagccatccagtttactttgcagggcttcccaaccttaccagagggcgccccagctggcaattccggttcgcttgctgtccataaaaccgcccagtctagctatcgccatgtaagcccactgcaagctacctgctttctctttgcgcttgcgttttcccttgtccagatagcccagtagctgacattcatccggggtcagcaccgtttctgcggactggctttctacgtggctgccatttttggggtgaggccgttcgcggccgaggggcgcagcccctggggggatgggaggcccgcgttagcgggccgggagggttcgagaagggggggcaccccccttcggcgtgcgcggtcacgcgcacagggcgcagccctggttaaaaacaaggtttataaatattggtttaaaagcaggttaaaagacaggttagcggtggccgaaaaacgggcggaaacccttgcaaatgctggattttctgcctgtggacagcccctcaaatgtcaataggtgcgcccctcatctgtcagcactctgcccctcaagtgtcaaggatcgcgcccctcatctgtcagtagtcgcgcccctcaagtgtcaataccgcagggcacttatccccaggcttgtccacatcatctgtgggaaactcgcgtaaaatcaggcgttttcgccgatttgcgaggctggccagctccacgtcgccggccgaaatcgagcctgcccctcatctgtcaacgccgcgccgggtgagtcggcccctcaagtgtcaacgtccgcccctcatctgtcagtgagggccaagttttccgcgaggtatccacaacgccggcggccctacatggctctgctgtagtgagtgggttgcgctccggcagcggtcctgatcccccgcagaaaaaaaggatctcaagaagatcctttgatcttttctac,misc_feature,#F58A5E,nucleotide -SEVA_RSF101,tcagcctgccgccttgggccgggtgatgtcgtacttgcccgccgcgaactcggttaccgtccagcccagcgcgaccagctccggcaacgcctcgcgcacccgctggcggcgcttgcgcatggtcgaaccactggcctctgacggccagacatagccgcacaaggtatctatggaagccttgccggttttgccggggtcgatccagccacacagccgctggtgcagcaggcgggcggtttcgctgtccagcgcccgcacctcgtccatgctgatgcgcacatgctggccgccacccatgacggcctgcgcgatcaaggggttcagggccacgtacaggcgcccgtccgcctcgtcgctggcgtactccgacagcagccgaaacccctgccgcttgcggccattctgggcgatgatggataccttccaaaggcgctcgatgcagtcctgtatgtgcttgagcgccccaccactatcgacctctgccccgatttcctttgccagcgcccgatagctacctttgaccacatggcattcagcggtgacggcctcccacttgggttccaggaacagccggagctgccgtccgccttcggtcttgggttccgggccaagcactaggccattaggcccagccatggccaccagcccttgcaggatgcgcagatcatcagcgcccagcggctccgggccgctgaactcgatccgcttgccgtcgccgtagtcatacgtcacgtccagcttgctgcgcttgcgctcgccccgcttgagggcacggaacaggccgggggccagacagtgcgccgggtcgtgccggacgtggctgaggctgtgcttgttcttaggcttcaccacggggcacccccttgctcttgcgctgcctctccagcacggcgggcttgagcaccccgccgtcatgccgcctgaaccaccgatcagcgaacggtgcgccatagttggccttgctcacaccgaagcggacgaagaaccggcgctggtcgtcgtccacaccccattcctcggcctcggcgctggtcatgctcgacaggtaggactgccagcggatgttatcgaccagtaccgagctgccccggctggcctgctgctggtcgcctgcgcccatcatggccgcgcccttgctggcatggtgcaggaacacgatagagcacccggtatcggcggcgatggcctccatgcgaccgatgacctgggccatggggccgctggcgttttcttcctcgatgtggaaccggcgcagcgtgtccagcaccatcaggcggcggccctcggcggcgcgcttgaggccgtcgaaccactccggggccatgatgttgggcaggctgccgatcagcggctggatcagcaggccgtcagccacggcttgccgttcctcggcgctgaggtgcgccccaagggcgtgcaggcggtgatgaatggcggtgggcgggtcttcggcgggcaggtagatcaccgggccggtgggcagttcgcccacctccagcagatccggcccgcctgcaatctgtgcggccagttgcagggccagcatggatttaccggcaccaccgggcgacaccagcgccccgaccgtaccggccaccatgttgggcaaaacgtagtccagcggtggcggcgctgctgcgaacgcctccagaatattgataggcttatgggtagccattgattgcctcctttgcaggcagttggtggttaggcgctggcggggtcactacccccgccctgcgccgctctgagttcttccaggcactcgcgcagcgcctcgtattcgtcgtcggtcagccagaacttgcgctgacgcatccctttggccttcatgcgctcggcatatcgcgcttggcgtacagcgtcagggctggccagcaggtcgccggtctgcttgtccttttggtctttcatatcagtcaccgagaaacttgccggggccgaaaggcttgtcttcgcggaacaaggacaaggtgcagccgtcaaggttaaggctggccatatcagcgactgaaaagcggccagcctcggccttgtttgacgtataaccaaagccaccgggcaaccaatagcccttgtcacttttgatcaggtagaccgaccctgaagcgcttttttcgtattccataaaacccccttctgtgcgtgagtactcatagtataacaggcgtgagtaccaacgcaagcactacatgctgaaatctggcccgcccctgtccatgcctcgctggcggggtgccggtgcccgtgccagctcggcccgcgcaagctggacgctgggcagacccatgaccttgctgacggtgcgctcgatgtaatccgcttcgtggccgggcttgcgctctgccagcgctgggctggcctcggccatggccttgccgatttcctcggcactgcggccccggctggccagcttctgcgcggcgataaagtcgcacttgctgaggtcatcaccgaagcgcttgaccagcccggccatctcgctgcggtactcgtccagcgccgtgcgccggtggcggctaagctgccgctcgggcagttcgaggctggccagcctgcgggccttctcctgctgccgctgggcctgctcgatctgctggccagcctgctgcaccagcgccgggccagcggtggcggtcttgcccttggattcacgcagcagcacccacggctgataaccggcgcgggtggtgtgcttgtccttgcggttggtgaagcccgccaagcggccatagtggcggctgtcggcgctggccgggtcggcgtcgtactcgctggccagcgtccgggcaatctgcccccgaagttcaccgcctgcggcgtcggccaccttgacccatgcctgatagttcttcgggctggtttccactaccagggcaggctcccggccctcggctttcatgtcatccaggtcaaactcgctgaggtcgtccaccagcaccagaccatgccgctcctgctcggcgggcctgatatacacgtcattgccctgggcattcatccgcttgagccatggcgtgttctggagcacttcggcggctgaccattcccggttcatcatctggccggtggtggcgtccctgacgccgatatcgaagcgctcacagcccatggccttgagctgtcggcctatggcctgcaaagtcctgtcgttcttcatcgggccaccaagcgattcccacacattatacgagccggaagcataaagtgtaaagcctagatccgaaggatgagccgggctgaatgatcgaccgagacaggccctgcggggctgcacacgcgcccccacccttcgggtagggggaaaggccgctaaagcggctaaaagcgctccagcgtatttctgcggggtttggtgtggggtttagcgggctttgcccgcctttccccctgccgcgcagcggtggggcggtgtgtagcctagcgcagcgaatagaccagctatccggcctctggccgggcatattgggcaagggcagcagcgccccacaagggcgctgataaccgcgcctagtggattattcttagataatcatggatggatttttccaacaccccgccagcccccgcccctgctgggtttgcaggtttgggggcgtgacagttattgcaggggttcgtgacagttattgcaggggggcgtgacagttattgcaggggttcgtgacagttag,misc_feature,#F58A5E,nucleotide +SEVA_RSF1010(x5ax),tcagcctgccgccttgggccgggtgatgtcgtacttgcccgccgcgaactcggttaccgtccagcccagcgcgaccagctccggcaacgcctcgcgcacccgctggcggcgcttgcgcatggtcgaaccactggcctctgacggccagacatagccgcacaaggtatctatggaagccttgccggttttgccggggtcgatccagccacacagccgctggtgcagcaggcgggcggtttcgctgtccagcgcccgcacctcgtccatgctgatgcgcacatgctggccgccacccatgacggcctgcgcgatcaaggggttcagggccacgtacaggcgcccgtccgcctcgtcgctggcgtactccgacagcagccgaaacccctgccgcttgcggccattctgggcgatgatggataccttccaaaggcgctcgatgcagtcctgtatgtgcttgagcgccccaccactatcgacctctgccccgatttcctttgccagcgcccgatagctacctttgaccaccatggcatcagcggtgacggcctcccacttgggttccaggaacagccggagctgccgtccgccttcggtcttgggttccgggccaagcactaggccattaggcccagccatggccaccagcccttgcaggatgcgcagatcatcagcgcccagcggctccgggccgctgaactcgatccgcttgccgtcgccgtagtcatacgtcacgtccagcttgctgcgcttgcgctcgccccgcttgagggcacggaacaggccgggggccagacagtgcgccgggtcgtgccggacgtggctgaggctgtgcttgttcttaggcttcaccacggggcacccccttgctcttgcgctgcctctccagcacggcgggcttgagcaccccgccgtcatgccgcctgaaccaccgatcagcgaacggtgcgccatagttggccttgctcacaccgaagcggacgaagaaccggcgctggtcgtcgtccacaccccattcctcggcctcggcgctggtcatgctcgacaggtaggactgccagcggatgttatcgaccagtaccgagctgccccggctggcctgctgctggtcgcctgcgcccatcatggccgcgcccttgctggcatggtgcaggaacacgatagagcacccggtatcggcggcgatggcctccatgcgaccgatgacctgggccatggggccgctggcgttttcttcctcgatgtggaaccggcgcagcgtgtccagcaccatcaggcggcggccctcggcggcgcgcttgaggccgtcgaaccactccggggccatgatgttgggcaggctgccgatcagcggctggatcagcaggccgtcagccacggcttgccgttcctcggcgctgaggtgcgccccaagggcgtgcaggcggtgatgaatggcggtgggcgggtcttcggcgggcaggtagatcaccgggccggtgggcagttcgcccacctccagcagatccggcccgcctgcaatctgtgcggccagttgcagggccagcatggatttaccggcaccaccgggcgacaccagcgccccgaccgtaccggccaccatgttgggcaaaacgtagtccagcggtggcggcgctgctgcgaacgcctccagaatattgataggcttatgggtagccattgattgcctcctttgcaggcagttggtggttaggcgctggcggggtcactacccccgccctgcgccgctctgagttcttccaggcactcgcgcagcgcctcgtattcgtcgtcggtcagccagaacttgcgctgacgcatccctttggccttcatgcgctcggcatatcgcgcttggcgtacagcgtcagggctggccagcaggtcgccggtctgcttgtccttttggtctttcatatcagtcaccgagaaacttgccggggccgaaaggcttgtcttcgcggaacaaggacaaggtgcagccgtcaaggttaaggctggccatatcagcgactgaaaagcggccagcctcggccttgtttgacgtataaccaaagccaccgggcaaccaatagcccttgtcacttttgatcaggtagaccgaccctgaagcgcttttttcgtattccataaaacccccttctgtgcgtgagtactcatagtataacaggcgtgagtaccaacgcaagcactacatgctgaaatctggcccgcccctgtccatgcctcgctggcggggtgccggtgcccgtgccagctcggcccgcgcaagctggacgctgggcagacccatgaccttgctgacggtgcgctcgatgtaatccgcttcgtggccgggcttgcgctctgccagcgctgggctggcctcggccatggccttgccgatttcctcggcactgcggccccggctggccagcttctgcgcggcgataaagtcgcacttgctgaggtcatcaccgaagcgcttgaccagcccggccatctcgctgcggtactcgtccagcgccgtgcgccggtggcggctaagctgccgctcgggcagttcgaggctggccagcctgcgggccttctcctgctgccgctgggcctgctcgatctgctggccagcctgctgcaccagcgccgggccagcggtggcggtcttgcccttggattcacgcagcagcacccacggctgataaccggcgcgggtggtgtgcttgtccttgcggttggtgaagcccgccaagcggccatagtggcggctgtcggcgctggccgggtcggcgtcgtactcgctggccagcgtccgggcaatctgcccccgaagttcaccgcctgcggcgtcggccaccttgacccatgcctgatagttcttcgggctggtttccactaccagggcaggctcccggccctcggctttcatgtcatccaggtcaaactcgctgaggtcgtccaccagcaccagaccatgccgctcctgctcggcgggcctgatatacacgtcattgccctgggcattcatccgcttgagccatggcgtgttctggagcacttcggcggctgaccattcccggttcatcatctggccggtggtggcgtccctgacgccgatatcgaagcgctcacagcccatggccttgagctgtcggcctatggcctgcaaagtcctgtcgttcttcatcgggccaccaagcgattcccacacattatacgagccggaagcataaagtgtagctagatccgaaggatgagccgggctgaatgatcgaccgagacaggccctgcggggctgcacacgcgcccccacccttcgggtagggggaaaggccgctaaagcggctaaaagcgctccagcgtatttctgcggggtttggtgtggggtttagcgggctttgcccgcctttccccctgccgcgcagcggtggggcggtgtgtagcctagcgcagcgaatagaccagctatccggcctctggccgggcatattgggcaagggcagcagcgccccacaagggcgctgataaccgcgcctagtggattattcttagataatcatggatggatttttccaacaccccgccagcccccgcccctgctgggtttgcaggtttgggggcgtgacagttattgcaggggttcgtgacagttattgcaggggggcgtgacagttattgcaggggttcgtgacagttag,misc_feature,#F58A5E,nucleotide SEVA_Sm/Sp,gaaccttgaccgaacgcagcggtggtaacggcgcagtggcggttttcatggcttgttatgactgtttttttggggtacagtctatgcctcgggcatccaagcagcaagcgcgttacgccgtgggtcgatgtttgatgttatggagcagcaacgatgttacgcagcagggcagtcgccctaaaacaaagttaaacatcatgagggaagcggtgatcgccgaagtatcgactcaactatcagaggtagttggcgtcatcgagcgccatctcgaaccgacgttgctggccgtacatttgtacggctccgcagtggatggcggcctgaagccacacagtgatattgatttgctggttacggtgaccgtaaggcttgatgaaacaacgcggcgagctttgatcaacgaccttttggaaacttcggcttcccctggagagagcgagattctccgcgctgtagaagtcaccattgttgtgcacgacgacatcattccgtggcgttatccagctaagcgcgaactgcaatttggagaatggcagcgcaatgacattcttgcaggtatcttcgagccagccacgatcgacattgatctggctatcttgctgacaaaagcaagagaacatagcgttgccttggtaggtccagcggcggaggaactctttgatccggttcctgaacaggatctatttgaggcgctaaatgaaaccttaacgctatggaactcgccgcccgactgggctggcgatgagcgaaatgtagtgcttacgttgtcccgcatttggtacagcgcagtaaccggcaaaatcgcgccgaaggatgtcgctgccgactgggcaatggagcgcctgccggcccagtatcagcccgtcatacttgaagctagacaggcttatcttggacaagaagaagatcgcttggcctcgcgcgcagatcagttggaagaatttgtccactacgtgaaaggcgagatcaccaaggtagtcggcaaataa,misc_feature,#F58A5E,nucleotide SEVA_T0,cttggactcctgttgatagatccagtaatgacctcagaactccatctggatttgttcagaacgctcggttgccgccgggcgttttttattggtgagaatccag,regulatory,#FAAC61,nucleotide SEVA_T1,tctagggcggcggatttgtcctactcaggagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcc,regulatory,#FAAC61,nucleotide -SEVA_Tet,ttgacagcttatcatcgataaactgtaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgaccattgtggccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctga,misc_feature,#F58A5E,nucleotide +SEVA_Tet(5axx),ttgacagcttatcatcgataaactgtaatgcggtagtttatcacagttaaattgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggattctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccctgcacgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgccgtccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgaccattgtggccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgagcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctga,misc_feature,#F58A5E,nucleotide \ No newline at end of file diff --git a/csv_xlsx_files/BASIC_SEVA_collection_v10_echo_scripts.zip b/csv_xlsx_files/BASIC_SEVA_collection_v10_echo_scripts.zip index 4a4bd91..9b21a59 100644 Binary files a/csv_xlsx_files/BASIC_SEVA_collection_v10_echo_scripts.zip and b/csv_xlsx_files/BASIC_SEVA_collection_v10_echo_scripts.zip differ diff --git a/csv_xlsx_files/addgene_headers.csv b/csv_xlsx_files/addgene_headers.csv new file mode 100644 index 0000000..0722af1 --- /dev/null +++ b/csv_xlsx_files/addgene_headers.csv @@ -0,0 +1,77 @@ +Plasmid Name,Plasmid Type,Purpose,PubMed ID,Gene or insert Name,Alternative Gene/Insert Name 1,Alternative Gene/Insert Name 2,Entrez Gene ID,gRNA/shRNA Sequence,Insert Size,Species of gene or insert,Additional Species of gene or insert,Species Other,Relevant Mutations,N-terminal Tag,C-terminal Tag,Backbone Name,Backbone Manufacturer,Primary Vector Type,Additional Vector Type 1,Additional Vector Type 2,Vector Type Other,Backbone size without insert,Cloning Method,Cloning Site 5-prime,5-prime Site Destroyed in Cloning,Cloning Site 3-prime,3-prime Site Destroyed in Cloning,Promoter,5-prime Sequencing Primer,3-prime Sequencing Primer,Bacterial Resistance,Bacterial Resistance Other,Selectable Marker,Selectable Marker Other,high or low copy,Growth Temp,Growth Strain,Growth Strain Other,Hazardous,Patents or Licenses,Patent/License Explanation,Gene Source,Comments,Sequence:Full,Sequence:partial 1 description,Sequence:partial 1,Sequence:partial 2 description,Sequence:partial 2 +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, 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+,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, +" If you need to deposit more than 75 plasmids, please email deposit@addgene.org to coordinate your deposit with an Addgene scientist",,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, diff --git a/pdfs/BASIC_SEVA_collection_v10_manual.pdf b/pdfs/BASIC_SEVA_collection_v10_manual.pdf index aba2707..1e96fd8 100644 Binary files a/pdfs/BASIC_SEVA_collection_v10_manual.pdf and b/pdfs/BASIC_SEVA_collection_v10_manual.pdf differ diff --git a/pdfs/bs_x7x_ts_oris_instructions.pdf b/pdfs/bs_x7x_ts_oris_instructions.pdf index 4366ebc..d4e0a10 100644 Binary files a/pdfs/bs_x7x_ts_oris_instructions.pdf and b/pdfs/bs_x7x_ts_oris_instructions.pdf differ diff --git a/scripts_nbs/addgene_submission.ipynb b/scripts_nbs/addgene_submission.ipynb index 7977856..4bcf7f0 100644 --- a/scripts_nbs/addgene_submission.ipynb +++ b/scripts_nbs/addgene_submission.ipynb @@ -2,6 +2,7 @@ "cells": [ { "cell_type": "markdown", + "metadata": {}, "source": [ "# basicsynbio Addgene submission collection\n", "\n", @@ -18,138 +19,231 @@ " - [x] pickled BasicLinker `BSEVA_L1`\n", " - [x] Selected Oris and AbR markers specified in #130.\n", " - [x] mScarlet counter selection casettes for associated Oris.\n", - "- [ ] Assemble BS_x7x_TS_CASc with BS_1xx to generate `BS_x7x_TS` and export build instructions. \n", + "- [x] Assemble BS_x7x_TS gblocks with BS_1xx to place them in storage vectors. Export associated data. \n", "- [x] Generate a dictionary mapping between Oris and counter-selection casettes.\n", - "- [ ] Make assemblies and export the following data:\n", + "- [x] Make assemblies and export the following data:\n", " - [x] Genbank file containing all correctly assembled backbones.\n", - " - [x] Manual and automated build instructions." - ], - "metadata": {} + " - [x] Manual and automated build instructions.\n", + "- [x] Calculate mass of AbR markers to add to clip reaction. *pJet contains multiple BsaI sites*.\n", + "\n", + "## Notes on implementation of above\n", + "\n", + "- Following commit ... x9x constructs were assembled with the 405 counter-selection cassette instead of the 407 cassette. This was in an attempt to lower the burden of these constructs." + ] }, { "cell_type": "code", - "execution_count": 12, + "execution_count": 21, + "metadata": {}, + "outputs": [], "source": [ "import basicsynbio as bsb\n", + "from basicsynbio.cam import seqrecord_hexdigest\n", "from basicsynbio.utils import MARKER_DICT, ORI_DICT\n", + "from Bio import SeqIO\n", + "import csv\n", + "import numpy as np\n", + "import pandas as pd\n", "from pathlib import Path\n", "import pickle\n", - "import re" - ], - "outputs": [], - "metadata": {} + "from platemap import Plate\n", + "import re\n", + "from typing import Tuple, Dict" + ] }, { "cell_type": "code", - "execution_count": 13, + "execution_count": 22, + "metadata": {}, + "outputs": [], "source": [ - "path_to_seqs = Path.cwd().parents[0] / \"sequences\"\n", - "path_to_initial_mods = path_to_seqs / \"genbank_files\" / \"BASIC_SEVA_collection\" / \"initial_mods\"\n", + "PATH_TO_SEQS = Path.cwd().parents[0] / \"sequences\"\n", + "PATH_TO_INITIAL_MODS = PATH_TO_SEQS / \"genbank_files\" / \"BASIC_SEVA_collection\" / \"initial_mods\"\n", "# Import objects\n", - "with open(path_to_seqs / \"alternative_formats\" / \"pickles\" / \"SEVA-BB1\", \"rb\") as file:\n", + "linkers = bsb.BASIC_BIOLEGIO_LINKERS[\"v0.1\"]\n", + "with open(PATH_TO_SEQS / \"alternative_formats\" / \"pickles\" / \"SEVA-BB1\", \"rb\") as file:\n", " bb_linker = pickle.load(file)\n", "abr_markers = list(\n", - " bsb.import_parts(path_to_initial_mods / \"AbR_markers.gb\",\n", + " bsb.import_parts(PATH_TO_INITIAL_MODS / \"AbR_markers.gb\",\n", " \"genbank\"\n", "))\n", - "seva_oris = list(\n", - " bsb.import_parts(path_to_initial_mods / \"SEVA_oris.gb\",\n", + "oris = list(\n", + " bsb.import_parts(PATH_TO_INITIAL_MODS / \"oris.gb\",\n", " \"genbank\"\n", "))\n", - "cs_cassettes = list(\n", - " bsb.import_parts(path_to_initial_mods / \"mScarlet_cs_cassettes.gb\",\n", + "cs_cassettes = bsb.import_parts(PATH_TO_INITIAL_MODS / \"mScarlet_cs_cassettes.gb\",\n", " \"genbank\"\n", - "))\n", - "ts_oris = list(bsb.import_parts(path_to_initial_mods / \"TS_ori_gblocks.gb\", \"genbank\"))" - ], - "outputs": [], - "metadata": {} + ")\n", + "cs_cassettes = [\n", + " bsb.BasicAssembly(cs_cassette.id, linkers[\"LMP\"], cs_cassette, linkers[\"LMS\"], bsb.BASIC_SEVA_PARTS[\"v0.1\"][\"18\"]).return_part(\n", + " id=cs_cassette.id,\n", + " name=cs_cassette.id,\n", + " ) for cs_cassette in cs_cassettes\n", + "]\n", + "ts_ori_gblocks = list(bsb.import_parts(PATH_TO_INITIAL_MODS / \"TS_ori_gblocks.gb\", \"genbank\"))\n", + "# Check for unwanted Benchling annotations\n", + "initial_modules = abr_markers + oris + cs_cassettes\n", + "unwanted_annotations = (\"Translation\", \"Primer\")\n", + "for initial_module in initial_modules:\n", + " if initial_module.id == \"BS_5axx\":\n", + " continue\n", + " qualifier_values = [value[0] for feature in initial_module.features for value in list(feature.qualifiers.values())]\n", + " for unwanted_annotation in unwanted_annotations:\n", + " re_matches = [re.match(unwanted_annotation, value[:len(unwanted_annotation)]) for value in qualifier_values]\n", + " if re_matches.count(None) != len(re_matches):\n", + " raise ValueError(f\"{initial_module.id} contains unwanted annotations\")" + ] }, { "cell_type": "code", - "execution_count": 18, + "execution_count": 23, + "metadata": {}, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "/home/hainesm6/.pyenv/versions/basicsynbio/lib/python3.8/site-packages/Bio/SeqIO/InsdcIO.py:726: BiopythonWarning: Increasing length of locus line to allow long name. This will result in fields that are not in usual positions.\n", + " warnings.warn(\n" + ] + }, + { + "data": { + "text/plain": [ + "'/home/hainesm6/github_repos/LondonBiofoundry/basicsynbio/pdfs/bs_x7x_ts_oris_instructions.pdf'" + ] + }, + "execution_count": 23, + "metadata": {}, + "output_type": "execute_result" + } + ], "source": [ "# Assemble BS_x7x_TS\n", - "linkers = bsb.BASIC_BIOLEGIO_LINKERS[\"v0.1\"]\n", "ts_ori_assemblies = [bsb.BasicAssembly(\n", " ts_ori.id,\n", " linkers[\"LMP\"],\n", " ts_ori,\n", " linkers[\"LMS\"],\n", " [marker for marker in abr_markers if marker.id == \"BS_1xx\"][0]\n", - ") for ts_ori in ts_oris]\n", + ") for ts_ori in ts_ori_gblocks]\n", "bsb.export_sequences_to_file(\n", " ts_ori_assemblies,\n", - " path_to_initial_mods / \"TS_ori_modules.gb\"\n", + " PATH_TO_INITIAL_MODS / \"TS_ori_modules.gb\"\n", ")\n", "bsb.pdf_instructions(\n", " bsb.BasicBuild(*ts_ori_assemblies),\n", - " \"/home/hainesm6/github_repos/LondonBiofoundry/basicsynbio/pdfs/bs_x7x_ts_instructions.pdf\",\n", - ")\n", - "ts_oris" - ], - "outputs": [ - { - "output_type": "execute_result", - "data": { - "text/plain": [ - "" - ] - }, - "metadata": {}, - "execution_count": 18 - } - ], - "metadata": {} + " \"/home/hainesm6/github_repos/LondonBiofoundry/basicsynbio/pdfs/bs_x7x_ts_oris_instructions.pdf\",\n", + ")" + ] }, { "cell_type": "code", - "execution_count": 8, + "execution_count": 24, + "metadata": {}, + "outputs": [], "source": [ + "def bseva_assembly(\n", + " marker: bsb.BasicPart,\n", + " ori: bsb.BasicPart,\n", + " cs_cassette: bsb.BasicPart,\n", + " id: str =\"foobar\",\n", + ") -> bsb.BasicAssembly:\n", + " \"\"\"Return a BASIC SEVA backbone as an assembly object.\n", + "\n", + " Args:\n", + " marker: Antibiotic resistance marker.\n", + " ori: Origin of replication.\n", + " cs_cassette: Counter-selection containing mScarlet CDS.\n", + " id: ID for assembly \n", + " \"\"\"\n", + " return bsb.BasicAssembly(\n", + " id,\n", + " linkers[\"LMP\"],\n", + " marker,\n", + " bb_linker,\n", + " ori,\n", + " linkers[\"LMS\"],\n", + " cs_cassette\n", + " )\n", + "\n", + "\n", + "def marker_abbreviation(marker_id: str) -> str:\n", + " \"\"\"Return abbreviation for resistance marker.\"\"\"\n", + " if re.match(\"BS_\\daxx\", marker_id) != None:\n", + " return abr_marker.id[-4:-2]\n", + " return abr_marker.id[-3]\n", + "\n", + "\n", "# Map oris with counter selection cassettes\n", "ori_cs_cassette_mapping = {\n", - " \"BS_x5x\": \"B407_J23119-RBS34-mScarl\",\n", + " \"BS_x5ax\": \"B407_J23119-RBS34-mScarl\",\n", " \"BS_x6x\": \"B407_J23119-RBS34-mScarl\",\n", " \"BS_x7x\": \"B408_J23119-RBS-A12-mSc\",\n", - " \"BS_x7x_TS\": \"B408_J23119-RBS-A12-mSc\",\n", - " \"BS_x8x\": \"B405_J23106-RBS34-mScarl\",\n", - " \"BS_x9x\": \"B407_J23119-RBS34-mScarl\",\n", + " \"BS_x7x_pKD46\": \"B408_J23119-RBS-A12-mSc\",\n", + " \"BS_x9x\": \"B405_J23106-RBS34-mScarl\",\n", "}\n", "ori_cs_cassette_mapping = {\n", " key: cs_cassettes[[cs_cassette.id for cs_cassette in cs_cassettes].index(value)] for key, value in ori_cs_cassette_mapping.items()\n", "}\n", - "# # Add TS ori to seva_oris\n", - "# oris = seva_oris\n", - "# oris.insert(3, bs_x7x_ts_assembly.return_part())\n", "# Make assemblies and export data\n", + "MARKER_DICT = MARKER_DICT.copy()\n", + "MARKER_DICT[\"5a\"] = \"Tetracycline\"\n", "assemblies = []\n", "for abr_marker in abr_markers:\n", + " abr_abbreviation = marker_abbreviation(abr_marker.id)\n", " for ori in oris: \n", - " assembly = bsb.BasicAssembly(\n", - " \"foobar\",\n", - " linkers[\"LMP\"],\n", + " assembly = bseva_assembly(\n", " abr_marker,\n", - " bb_linker,\n", " ori,\n", - " linkers[\"LMS\"],\n", - " ori_cs_cassette_mapping[ori.id],\n", + " ori_cs_cassette_mapping[ori.id]\n", " )\n", - " if ori.id == \"BS_x7x_TS\":\n", - " assembly.id = f\"BASIC_SEVA_{abr_marker.id[-3]}7_TS.10\"\n", - " assembly.description = f\"BASIC SEVA vector containing {MARKER_DICT[abr_marker.id[-3]]} resistance marker and temperature sensitive pSC101 origin of replication.\"\n", + " assembly.marker = MARKER_DICT[abr_abbreviation] \n", + " if ori.id == \"BS_x7x_pKD46\":\n", + " assembly.abbreviation = f\"{abr_abbreviation}7_pKD46\"\n", + " assembly.ori = \"temperature sensitive pSC101\"\n", + " elif re.match(\"BS_x\\dax\", ori.id):\n", + " assembly.abbreviation = abr_abbreviation + ori.id[-3:-1]\n", + " assembly.ori = ORI_DICT[ori.id[-3]] \n", " else:\n", " assert re.match(\"BS_x\\dx\", ori.id) != None\n", - " assembly.id = f\"BASIC_SEVA_{abr_marker.id[-3] + ori.id[-2]}.10\"\n", - " assembly.description = f\"BASIC SEVA vector containing {MARKER_DICT[abr_marker.id[-3]]} resistance marker and {ORI_DICT[ori.id[-2]]} origin of replication.\"\n", + " assembly.abbreviation = abr_abbreviation + ori.id[-2]\n", + " assembly.ori = ORI_DICT[ori.id[-2]]\n", + " assembly.id = f\"BASIC_SEVA_{assembly.abbreviation}.10\"\n", + " assembly.description = f\"BASIC SEVA vector containing {assembly.marker} resistance marker and {assembly.ori} origin of replication\"\n", " assemblies.append(assembly)\n", "build = bsb.BasicBuild(*assemblies)\n", + "PATH_TO_CSVS = Path.cwd().parents[0] / \"csv_xlsx_files\"\n" + ] + }, + { + "cell_type": "code", + "execution_count": 25, + "metadata": {}, + "outputs": [ + { + "data": { + "text/plain": [ + "'/home/hainesm6/github_repos/LondonBiofoundry/basicsynbio/pdfs/BASIC_SEVA_collection_v10_manual.pdf'" + ] + }, + "execution_count": 25, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ "bsb.export_sequences_to_file(\n", - " (assembly.return_part(description=assembly.description, name=assembly.id) for assembly in build.basic_assemblies),\n", + " (assembly.return_part(\n", + " description=assembly.description,\n", + " name=assembly.id,\n", + " id=seqrecord_hexdigest(assembly.return_part())\n", + " ) for assembly in build.basic_assemblies),\n", " Path.cwd().parents[0] / \"basicsynbio\" / \"parts_linkers\" / \"BASIC_SEVA_collection_v10.gb\"\n", - ")\n", + ") \n", "bsb.export_echo_assembly(\n", " build,\n", - " Path.cwd().parents[0] / \"csv_xlsx_files\" / \"BASIC_SEVA_collection_v10_echo_scripts.zip\",\n", + " PATH_TO_CSVS / \"BASIC_SEVA_collection_v10_echo_scripts.zip\",\n", " assemblies_per_clip=20,\n", " alternate_well=True,\n", ")\n", @@ -157,45 +251,254 @@ " build,\n", " \"/home/hainesm6/github_repos/LondonBiofoundry/basicsynbio/pdfs/BASIC_SEVA_collection_v10_manual.pdf\",\n", " assemblies_per_clip=20,\n", - ")\n" - ], + ")" + ] + }, + { + "cell_type": "code", + "execution_count": 26, + "metadata": {}, + "outputs": [], + "source": [ + "# Calculate AbR marker mass to add to clip reaction\n", + "PJET_LEN = 2974\n", + "def approx_clip_mass(seq_length: int, ndigit: int =None) -> float:\n", + " \"\"\"Calculate the approximate mass of a BASIC part (ng) to add to a clip reaction.\"\"\"\n", + " return round(2.5*30e-6*(seq_length*607.4 + 157.9), ndigit)\n", + "abr_masses = {\n", + " \"Marker ID\": [marker.id for marker in abr_markers],\n", + " \"mass (ng)\": [\n", + " approx_clip_mass(PJET_LEN + len(marker.seq)) for marker in abr_markers\n", + " ],\n", + "}\n", + "abr_masses = pd.DataFrame(abr_masses)\n", + "abr_masses.to_csv(\n", + " PATH_TO_CSVS / \"AbR_marker_clip_masses.csv\",\n", + " index=False,\n", + ")" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Results and discussion\n", + "\n", + "- It has previously been reported that inhibition of protein synthesis dramatically increases the copy number of relaxed plasmids such as pBR322-ROP (Sambrook). This would lead to relatively higher expression of mScarlet compared to antibiotics that don't inhibit protein synthesis e.g. carbenicilin. This could explain why certain x9x constructs were burdensome. \n", + "- Lowering the concentration of tetracycline to 5 microgram/mL may improve the growth rate of BASIC_SEVA_5a5a.10, 5a7 and presumably 5a7_pKD46.10. All these plasmids replicate via a stringent mechanism and therefore cannot increase copy number if protein expression is blocked. This could explain why dropping the concentration improved the growth of these plasmids.\n", + "\n" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Aims and objectives for the cell/s below\n", + "\n", + "- [x] Export csv file containing the following data for plasmids cultured at 37 degrees Celsius: name, marker, transformation well, overnight well.\n", + "- [x] Export a csv file containing data on BsaI digested constructs in the form: Assembly ID, Fragment 1 Length (bp), Fragment 2 Length (bp).\n", + "- [x] Generate a submission file for addgene for the collection.\n", + "- [X] Export `assembly.abbreviation` values to a spreadsheet.\n", + "- [ ] What is the mean size of the plasmid in the collection." + ] + }, + { + "cell_type": "code", + "execution_count": 27, + "metadata": {}, + "outputs": [], + "source": [ + "def construct_picking_dict(\n", + " overnight_wells: Tuple[str, ...],\n", + " transformation_well: str,\n", + " marker: str,\n", + " name: str,\n", + ") -> Dict:\n", + " \"\"\"Return dictionary describing wells construct is picked into.\"\"\"\n", + " return [{\n", + " \"Overnight Well\": overnight_well,\n", + " \"Transformation Well\": transformation_well,\n", + " \"Marker\": marker,\n", + " \"Name\": name,\n", + " } for overnight_well in overnight_wells]\n", + "\n", + "\n", + "PLATE_96 = Plate(size=96)\n", + "\n", + "\n", + "def plate_wells(\n", + " number_of_wells: str,\n", + " first_well_index: int,\n", + " plate: Plate =PLATE_96, \n", + "):\n", + " \"\"\"slice wells of plate based on number of wells required and first well index.\"\"\"\n", + " return plate.wells[first_well_index:first_well_index + number_of_wells]\n", + "\n", + "\n", + "overnight_ind = 0\n", + "NUMBER_OF_WELLS_TO_PICK = 4\n", + "with open(PATH_TO_CSVS / \"BASIC_SEVA_10_overnights.csv\", \"w\", newline=\"\") as csvf:\n", + " fieldnames = [\"Overnight Well\", \"Transformation Well\", \"Marker\", \"Name\",]\n", + " dictwriter = csv.DictWriter(csvf, fieldnames=fieldnames)\n", + " dictwriter.writeheader()\n", + " for ind, assembly in enumerate(build.basic_assemblies):\n", + " if re.match(\"BASIC_SEVA_\\d7_pKD46.10\", assembly.id):\n", + " continue\n", + " elif re.match(\"BASIC_SEVA_\\d[a-z]7_pKD46.10\", assembly.id):\n", + " continue\n", + " dictwriter.writerows(construct_picking_dict(\n", + " overnight_wells=plate_wells(\n", + " NUMBER_OF_WELLS_TO_PICK,\n", + " first_well_index=overnight_ind\n", + " ),\n", + " transformation_well=PLATE_96.wells[ind],\n", + " marker=assembly.marker,\n", + " name=assembly.id\n", + " ))\n", + " overnight_ind += NUMBER_OF_WELLS_TO_PICK" + ] + }, + { + "cell_type": "code", + "execution_count": 28, + "metadata": {}, + "outputs": [], + "source": [ + "with open(PATH_TO_CSVS / \"BASIC_SEVA_10_bsaI_digest.csv\", \"w\", newline=\"\") as csvf:\n", + " fieldnames = [\"Assembly ID\", \"Fragment 1 Length (bp)\", \"Fragment 2 Length (bp)\"]\n", + " dictwriter = csv.DictWriter(csvf, fieldnames=fieldnames)\n", + " dictwriter.writeheader()\n", + " for assembly_digest in bsb.build_digest(build):\n", + " dictwriter.writerow({\n", + " \"Assembly ID\": assembly_digest.assembly.id,\n", + " \"Fragment 1 Length (bp)\": assembly_digest.product_lengths[0],\n", + " \"Fragment 2 Length (bp)\": assembly_digest.product_lengths[1]\n", + " })" + ] + }, + { + "cell_type": "code", + "execution_count": 29, + "metadata": {}, + "outputs": [], + "source": [ + "addgene_template = pd.read_csv(PATH_TO_CSVS / \"addgene_headers.csv\")\n", + "ADDGENE_FIELDNAMES = tuple(name for name in addgene_template.columns)\n", + "rows = [\n", + " {fieldname: \"\" for fieldname in ADDGENE_FIELDNAMES} for i in range(len(build.basic_assemblies))\n", + "]\n", + "for ind, assembly_digest in enumerate(bsb.build_digest(build)):\n", + " rows[ind].update(\n", + " {\n", + " \"Plasmid Name\": assembly_digest.assembly.id,\n", + " \"Plasmid Type\": \"Encodes one insert\",\n", + " \"Purpose\": \"BASIC DNA assembly vector\",\n", + " \"Gene or insert Name\": \"mScarlet counter-selection cassette\",\n", + " \"Insert Size\": min(assembly_digest.product_lengths),\n", + " \"Species of gene or insert\": \"Synthetic\",\n", + " \"Relevant Mutations\": \"Not applicable\",\n", + " \"Backbone Name\": assembly_digest.assembly.id,\n", + " \"Primary Vector Type\": \"Synthetic Biology\",\n", + " \"Backbone size without insert\": max(assembly_digest.product_lengths),\n", + " \"Cloning Method\": \"Unknown\",\n", + " \"5-prime Sequencing Primer\": \"ggcggcggatttgtcctac\",\n", + " \"3-prime Sequencing Primer\": \"ggtgagaatccaggggtcc\",\n", + " \"Bacterial Resistance\": \"Spectinomycin\" if assembly_digest.assembly.marker == \"Streptomycin/Spectinomycin\" else assembly_digest.assembly.marker,\n", + " \"high or low copy\": \"Low Copy\",\n", + " \"Growth Temp\": \"30 C\" if assembly_digest.assembly.ori == \"temperature sensitive pSC101\" else \"37 C\",\n", + " \"Growth Strain\": \"DH5alpha\",\n", + " \"Hazardous\": \"No\",\n", + " \"Patents or Licenses\": \"No\",\n", + " \"Comments\": \"Cloning Method: BASIC DNA assembly\",\n", + " \"Sequence:Full\": str(assembly_digest.assembly.return_seqrec().seq)\n", + " }\n", + " )\n", + "with open(PATH_TO_CSVS / \"BASIC_SEVA_10_addgene_batch_upload.csv\", \"w\", newline=\"\") as csvf:\n", + " writer = csv.DictWriter(csvf, ADDGENE_FIELDNAMES)\n", + " writer.writeheader()\n", + " writer.writerows(rows) \n" + ] + }, + { + "cell_type": "code", + "execution_count": 30, + "metadata": {}, + "outputs": [], + "source": [ + "abbreviation_df = pd.DataFrame(\n", + " {\"Abbreviations\": [assembly.abbreviation for assembly in build.basic_assemblies]}\n", + ")\n", + "abbreviation_df.to_csv(\n", + " PATH_TO_CSVS / \"BASIC_SEVA_10_abbreviations.csv\",\n", + " index=False\n", + ")" + ] + }, + { + "cell_type": "code", + "execution_count": 31, + "metadata": {}, "outputs": [ { - "output_type": "execute_result", - "data": { - "text/plain": [ - "'/home/hainesm6/github_repos/LondonBiofoundry/basicsynbio/pdfs/BASIC_SEVA_collection_v10_manual.pdf'" - ] - }, - "metadata": {}, - "execution_count": 8 + "name": "stdout", + "output_type": "stream", + "text": [ + "Mean plasmid size: 4025.4\n" + ] } ], - "metadata": {} + "source": [ + "print(f\"Mean plasmid size: {np.mean(np.array([len(assembly.return_part()) for assembly in build.basic_assemblies]))}\")" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Aims/objectives for cell/s below\n", + "\n", + "- [x] Export sequence flanked by *i*P and *i*S regions in oris for use by [cmatch](https://github.com/kitneylab/cmatch) to validate assemblies." + ] + }, + { + "cell_type": "code", + "execution_count": 35, + "metadata": {}, + "outputs": [], + "source": [ + "core_ori_seqs = [ori.basic_slice() for ori in oris]\n", + "for core_ori_seq in core_ori_seqs:\n", + " SeqIO.write(\n", + " core_ori_seq,\n", + " PATH_TO_SEQS / \"genbank_files\" / \"BASIC_SEVA_collection\" / \"misc_seqs\" / f\"{core_ori_seq.id}_core_seq.gb\",\n", + " \"genbank\"\n", + " )\n", + " " + ] } ], "metadata": { - "orig_nbformat": 4, + "interpreter": { + "hash": "1f21550770ffb19e127f11e22c269d225bc263a3841b68cd3096e7dc8f6b378e" + }, + "kernelspec": { + "display_name": "Python 3.8.5 64-bit ('basicsynbio': pyenv)", + "name": "python3" + }, "language_info": { - "name": "python", - "version": "3.8.5", - "mimetype": "text/x-python", "codemirror_mode": { "name": "ipython", "version": 3 }, - "pygments_lexer": "ipython3", + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", "nbconvert_exporter": "python", - "file_extension": ".py" - }, - "kernelspec": { - "name": "python3", - "display_name": "Python 3.8.5 64-bit ('basicsynbio': pyenv)" + "pygments_lexer": "ipython3", + "version": "3.8.5" }, - "interpreter": { - "hash": "a9ef394515f7bdfc1a2fdab82219cc959d35a48d1a80fecda128203466856ae6" - } + "orig_nbformat": 4 }, "nbformat": 4, "nbformat_minor": 2 -} \ No newline at end of file +} diff --git a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/AbR_markers.gb b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/AbR_markers.gb index 698effa..381215f 100644 --- a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/AbR_markers.gb +++ b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/AbR_markers.gb @@ -1,4 +1,4 @@ -LOCUS BS_1xx 1220 bp ds-DNA linear 29-JUL-2021 +LOCUS BS_1xx 1220 bp ds-DNA linear 15-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 25..127 @@ -28,7 +28,7 @@ ORIGIN 1141 attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg acgacccttg 1201 tcGGCTCGGG AGACCTATCG // -LOCUS BS_2xx 1108 bp ds-DNA linear 29-JUL-2021 +LOCUS BS_2xx 1108 bp ds-DNA linear 15-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 25..127 @@ -56,7 +56,7 @@ ORIGIN 1021 CGGATATGAA CAAACTGCAA TTTCATCTGA TGCTGGATGA ATTTTTCTAA TAATTAATTG 1081 GACCGCGGTC GGCTCGGGAG ACCTATCG // -LOCUS BS_3xx 964 bp ds-DNA linear 29-JUL-2021 +LOCUS BS_3xx 964 bp ds-DNA linear 15-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 25..127 @@ -82,7 +82,7 @@ ORIGIN 901 cagtactgcg atgagtggca gggcggggcg taatttgact tttgtcGGCT CGGGAGACCT 961 ATCG // -LOCUS BS_4xx 1178 bp ds-DNA linear 29-JUL-2021 +LOCUS BS_4xx 1178 bp ds-DNA linear 15-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 25..127 @@ -111,13 +111,13 @@ ORIGIN 1081 gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt 1141 cggcaaataa gacaattgtc GGCTCGGGAG ACCTATCG // -LOCUS BS_5xx 1456 bp ds-DNA linear 29-JUL-2021 +LOCUS BS_5axx 1456 bp ds-DNA linear 15-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 25..127 /label="SEVA_T0" misc_feature 162..1428 - /label="SEVA_Tet" + /label="SEVA_Tet(5axx)" ORIGIN 1 TCTGGTGGGT CTCTGTCCac tagtcttgga ctcctgttga tagatccagt aatgacctca 61 gaactccatc tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag @@ -135,7 +135,7 @@ ORIGIN 781 caggagtcgc ataagggaga gcgccgtccg atgcccttga gagccttcaa cccagtcagc 841 tccttccggt gggcgcgggg catgaccatt gtggccgcac ttatgactgt cttctttatc 901 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt - 961 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc + 961 cgctggagcg cgacgatgag cggcctgtcg cttgcggtat tcggaatctt gcacgccctc 1021 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc 1081 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg 1141 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag @@ -145,7 +145,7 @@ ORIGIN 1381 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaga caattgtcGG 1441 CTCGGGAGAC CTATCG // -LOCUS BS_6xx 986 bp ds-DNA linear 29-JUL-2021 +LOCUS BS_6xx 986 bp ds-DNA linear 15-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 25..127 diff --git a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/SEVA_oris.gb b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/SEVA_oris.gb deleted file mode 100644 index 2c663e9..0000000 --- a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/SEVA_oris.gb +++ /dev/null @@ -1,343 +0,0 @@ -LOCUS BS_x5x 5108 bp ds-DNA circular 13-SEP-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 1..57 - /label="LMP" - misc_feature 66..3739 - /label="SEVA_RSF101" - misc_feature 3754..3858 - /label="SEVA_T1" - misc_feature 3868..3924 - /label="LMS" - misc_feature 3931..4033 - /label="SEVA_T0" - misc_feature 4060..5098 - /label="SEVA_Ap" -ORIGIN - 1 GGCTCGGGTA AGAACTCGCA CTTCGTGGAA ACACTATTAT CTGGTGGGTC TCTGTCCggc - 61 cggcctcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt - 121 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg - 181 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa - 241 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg - 301 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc - 361 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg - 421 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg - 481 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg - 541 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac atggcattca - 601 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc - 661 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg - 721 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg - 781 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg - 841 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg - 901 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg - 961 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt - 1021 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc - 1081 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag - 1141 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt - 1201 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg - 1261 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag - 1321 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc - 1381 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac - 1441 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc - 1501 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc - 1561 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc - 1621 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta - 1681 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc - 1741 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg - 1801 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc - 1861 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg - 1921 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata - 1981 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt - 2041 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt - 2101 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta - 2161 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc - 2221 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct - 2281 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac - 2341 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg - 2401 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact - 2461 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc - 2521 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg - 2581 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg - 2641 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc - 2701 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg - 2761 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc - 2821 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc - 2881 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag - 2941 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac - 3001 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc - 3061 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt - 3121 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc - 3181 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga - 3241 ttcccacaca ttatacgagc cggaagcata aagtgtaaag cctagatccg aaggatgagc - 3301 cgggctgaat gatcgaccga gacaggccct gcggggctgc acacgcgccc ccacccttcg - 3361 ggtaggggga aaggccgcta aagcggctaa aagcgctcca gcgtatttct gcggggtttg - 3421 gtgtggggtt tagcgggctt tgcccgcctt tccccctgcc gcgcagcggt ggggcggtgt - 3481 gtagcctagc gcagcgaata gaccagctat ccggcctctg gccgggcata ttgggcaagg - 3541 gcagcagcgc cccacaaggg cgctgataac cgcgcctagt ggattattct tagataatca - 3601 tggatggatt tttccaacac cccgccagcc cccgcccctg ctgggtttgc aggtttgggg - 3661 gcgtgacagt tattgcaggg gttcgtgaca gttattgcag gggggcgtga cagttattgc - 3721 aggggttcgt gacagttagg gcgcgcccag ctgtctaggg cggcggattt gtcctactca - 3781 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct - 3841 ttcgttttat ttgatgcctt taattaaGGC TCGGGAGACC TATCGGTAAT AACAGTCCAA - 3901 TCTGGTGTAA CTTCGGAATC GTCCactagt cttggactcc tgttgataga tccagtaatg - 3961 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt - 4021 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg - 4081 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc - 4141 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca - 4201 ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg - 4261 cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg - 4321 gctatattga actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac - 4381 gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg - 4441 atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat - 4501 atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg - 4561 cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc - 4621 cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt - 4681 gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag - 4741 caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc - 4801 agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc - 4861 tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca - 4921 ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg - 4981 gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga - 5041 ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga - 5101 cccttgtc -// -LOCUS BS_x6x 2143 bp ds-DNA circular 13-SEP-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 594..650 - /label="LMP" - misc_feature 659..1390 - /label="SEVA_p15A" - misc_feature 1405..1509 - /label="SEVA_T1" - misc_feature 1519..1575 - /label="LMS" - misc_feature 1582..1684 - /label="SEVA_T0" - misc_feature join(1711..2143,1..583) - /label="SEVA_Ap" -ORIGIN - 1 aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga - 61 gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg - 121 cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga - 181 acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc - 241 tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt - 301 ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt - 361 ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg - 421 gcccggatgg taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga - 481 tggatgaacg taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa - 541 ccgatacaat taaaggctcc ttttggagcc tttttttttg gacgaccctt gtcGGCTCGG - 601 GTAAGAACTC GCACTTCGTG GAAACACTAT TATCTGGTGG GTCTCTGTCC ggccggccct - 661 agaaatattt tatctgatta ataagatgat cttcttgaga tcgttttggt ctgcgcgtaa - 721 tctcttgctc tgaaaacgaa aaaaccgcct tgcagggcgg tttttcgaag gttctctgag - 781 ctaccaactc tttgaaccga ggtaactggc ttggaggagc gcagtcacca aaacttgtcc - 841 tttcagttta gccttaaccg gcgcatgact tcaagactaa ctcctctaaa tcaattacca - 901 gtggctgctg ccagtggtgc ttttgcatgt ctttccgggt tggactcaag acgatagtta - 961 ccggataagg cgcagcggtc ggactgaacg gggggttcgt gcatacagtc cagcttggag - 1021 cgaactgcct acccggaact gagtgtcagg cgtggaatga gacaaacgcg gccataacag - 1081 cggaatgaca ccggtaaacc gaaaggcagg aacaggagag cgcacgaggg agccgccagg - 1141 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca - 1201 gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct - 1261 ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa - 1321 gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg - 1381 gaatatatcc GGCGCGCCCA GCTGTCTAGG GCGGCGGATT TGTCCTACTC AGGAGAGCGT - 1441 TCACCGACAA ACAACAGATA AAACGAAAGG CCCAGTCTTT cgactgagcc tttcgtttta - 1501 tttgatgcct ttaattaaGG CTCGGGAGAC CTATCGGTAA TAACAGTCCA ATCTGGTGTA - 1561 ACTTCGGAAT CGTCCactag tcttggactc ctgttgatag atccagtaat gacctcagaa - 1621 ctccatctgg atttgttcag aacgctcggt tgccgccggg cgttttttat tggtgagaat - 1681 ccaggggtcc ccaataatta cgatttaaat tagtagcccg cctaatgagc gggctttttt - 1741 ttaattcccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac - 1801 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagc attcagcatt - 1861 ttcgtgtggc gctgattccg ttttttgcgg cgttttgcct gccggtgttt gcgcatccgg - 1921 aaaccctggt gaaagtgaaa gatgcggaag atcaactggg tgcgcgcgtg ggctatataa - 1981 attctggaat cttttcgtcc ggaagaacgt tttccgatga tgagcacctt taaagtgctg - 2041 ctgtgcggtg cggttctgag ccgtgtggat gcgggccagg aacaactggg ccgtcgtatt - 2101 cattatagcc agaacgatct ggtggaatat agcccggtga ccg -// -LOCUS BS_x7x 2894 bp ds-DNA circular 13-SEP-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 618..674 - /label="LMP" - misc_feature 683..2142 - /label="SEVA_pSC101" - misc_feature 2157..2261 - /label="SEVA_T1" - misc_feature 2271..2327 - /label="LMS" - misc_feature 2334..2436 - /label="SEVA_T0" - misc_feature join(2463..2894,1..607) - /label="SEVA_Ap" -ORIGIN - 1 tggtggaata tagcccggtg accgaaaaac atctgaccga tggcatgacc gtgcgtgaac - 61 tgtgcagcgc ggcgattacc atgagcgata acaccgcggc gaacctgctg ctgacgacca - 121 ttggcggtcc gaaagaactg accgcgtttc tgcataacat gggcgatcat gtgacccgtc - 181 tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa cgatgaacgt gataccacca - 241 tgccggcagc aatggcgacc accctgcgta aactgctgac gggtgagctg ctgaccctgg - 301 caagccgcca gcaactgatt gattggatgg aagcggataa agtggcgggt ccgctgctgc - 361 gtagcgcgct gccggctggc tggtttattg cggataaaag cggtgcgggc gaacgtggca - 421 gccgtggcat tattgcggcg ctgggcccgg atggtaaacc gagccgtatt gtggtgattt - 481 ataccaccgg cagccaggcg acgatggatg aacgtaaccg tcagattgcg gaaattggcg - 541 cgagcctgat taaacattgg taaaccgata caattaaagg ctccttttgg agcctttttt - 601 tttggacgac ccttgtcGGC TCGGGTAAGA ACTCGCACTT CGTGGAAACA CTATTATCTG - 661 GTGGGTCTCT GTCCggccgg cctcagatcc ttccgTATTT AGCCAGTATG TTCTCTAGTG - 721 TGGTTCGTTG TTTTTGCGTG AGCCATGAGA ACGAACCATT GAGATCATAC TTACTTTGCA - 781 TGTCACTCAA AAATTTTGCC TCAAAACTGG TGAGCTGAAT TTTTGCAGTT AAAGCATCGT - 841 GTAGTGTTTT TCTTAGTCCG TTATGTAGGT AGGAATCTGA TGTAATGGTT GTTGGTATTT - 901 TGTCACCATT CATTTTTATC TGGTTGTTCT CAAGTTCGGT TACGAGATCC ATTTGTCTAT - 961 CTAGTTCAAC TTGGAAAATC AACGTATCAG TCGGGCGGCC TCGCTTATCA ACCACCAATT - 1021 TCATATTGCT GTAAGTGTTT AAATCTTTAC TTATTGGTTT CAAAACCCAT TGGTTAAGCC - 1081 TTTTAAACTC ATGGTAGTTA TTTTCAAGCA TTAACATGAA CTTAAATTCA TCAAGGCTAA - 1141 TCTCTATATT TGCCTTGTGA GTTTTCTTTT GTGTTAGTTC TTTTAATAAC CACTCATAAA - 1201 TCCTCATAGA GTATTTGTTT TCAAAAGACT TAACATGTTC CAGATTATAT TTTATGAATT - 1261 TTTTTAACTG GAAAAGATAA GGCAATATCT CTTCACTAAA AACTAATTCT AATTTTTCGC - 1321 TTGAGAACTT GGCATAGTTT GTCCACTGGA AAATCTCAAA GCCTTTAACC AAAGGATTCC - 1381 TGATTTCCAC AGTTCTCGTC ATCAGCTCTC TGGTTGCTTT AGCTAATACA CCATAAGCAT - 1441 TTTCCCTACT GATGTTCATC ATCTGAGCGT ATTGGTTATA AGTGAACGAT ACCGTCCGTT - 1501 CTTTCCTTGT AGGGTTTTCA ATCGTGGGGT TGAGTAGTGC CACACAGCAT AAAATTAGCT - 1561 TGGTTTCATG CTCCGTTAAG TCATAGCGAC TAATCGCTAG TTCATTTGCT TTGAAAACAA - 1621 CTAATTCAGA CATACATCTC AATTGGTCTA GGTGATTTTA ATCACTATAC CAATTGAGAT - 1681 GGGCTAGTCA ATGATAATTA CATGTCCTTT TCCTTTGAGT TGTGGGTATC TGTAAATTCT - 1741 GCTAGACCTT TGCTGGAAAA CTTGTAAATT CTGCTAGACC CTCTGTAAAT TCCGCTAGAC - 1801 CTTTGTGTGT TTTTTTTGTT TATATTCAAG TGGTTATAAT TTATAGAATA AAGAAAGAAT - 1861 AAAAAAAGAT AAAAAGAATA GATCCCAGCC CTGTGTATAA CTCACTACTT TAGTCAGTTC - 1921 CGCAGTATTA CAAAAGGATG TCGCAAACGC TGTTTGCTCC TCTACAAAAC AGACCTTAAA - 1981 ACCCTAAAGG CTTAAGTAGC ACCCTCGCAA GCTCGGGCAA ATCGCTGAAT ATTCCTTTTG - 2041 TCTCCGACCA TCAGGCACCT GAGTCGCTGT CTTTTTCGTG ACATTCAGTT CGCTGCGCTC - 2101 ACGGCTCTGG CAGTGAATGG GGGTAAATGG CACTACAGGC GCGGCGCGCC CAGCTGTCTA - 2161 GGGCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA TAAAACGAAA - 2221 GGCCCAGTCT TTcgactgag cctttcgttt tatttgatgc ctttaattaa GGCTCGGGAG - 2281 ACCTATCGGT AATAACAGTC CAATCTGGTG TAACTTCGGA ATCGTCCact agtcttggac - 2341 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg - 2401 gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat tacgatttaa - 2461 attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt tatttttcta - 2521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata - 2581 ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc cgttttttgc - 2641 ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga aagatgcgga - 2701 agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg gcaaaattct - 2761 ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag tgctgctgtg - 2821 cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc gtattcatta - 2881 tagccagaac gatc -// -LOCUS BS_x8x 2363 bp ds-DNA circular 13-SEP-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 60..988 - /label="SEVA_pUC" - misc_feature 1003..1107 - /label="SEVA_T1" - misc_feature 1117..1173 - /label="LMS" - misc_feature 1180..1282 - /label="SEVA_T0" - misc_feature 1309..2347 - /label="SEVA_Ap" - misc_feature join(2358..2363,1..51) - /label="LMP" -ORIGIN - 1 GGTAAGAACT CGCACTTCGT GGAAACACTA TTATCTGGTG GGTCTCTGTC Cggccggccc - 61 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt - 121 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac - 181 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt - 241 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct - 301 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga - 361 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac - 421 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctttg - 481 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt - 541 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc - 601 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg - 661 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc - 721 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc - 781 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag - 841 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca - 901 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat - 961 taatgtgagt tagctcactc attaggcaGG CGCGCCCAGC TGTCTAGGGC GGCGGATTTG - 1021 TCCTACTCAG GAGAGCGTTC ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTcg - 1081 actgagcctt tcgttttatt tgatgccttt aattaaGGCT CGGGAGACCT ATCGGTAATA - 1141 ACAGTCCAAT CTGGTGTAAC TTCGGAATCG TCCactagtc ttggactcct gttgatagat - 1201 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg - 1261 ttttttattg gtgagaatcc aggggtcccc aataattacg atttaaatta gtagcccgcc - 1321 taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata - 1381 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga - 1441 gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt ttttgcggcg ttttgcctgc - 1501 cggtgtttgc gcatccggaa accctggtga aagtgaaaga tgcggaagat caactgggtg - 1561 cgcgcgtggg ctatattgaa ctggatctga acagcggcaa aattctggaa tcttttcgtc - 1621 cggaagaacg ttttccgatg atgagcacct ttaaagtgct gctgtgcggt gcggttctga - 1681 gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat tcattatagc cagaacgatc - 1741 tggtggaata tagcccggtg accgaaaaac atctgaccga tggcatgacc gtgcgtgaac - 1801 tgtgcagcgc ggcgattacc atgagcgata acaccgcggc gaacctgctg ctgacgacca - 1861 ttggcggtcc gaaagaactg accgcgtttc tgcataacat gggcgatcat gtgacccgtc - 1921 tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa cgatgaacgt gataccacca - 1981 tgccggcagc aatggcgacc accctgcgta aactgctgac gggtgagctg ctgaccctgg - 2041 caagccgcca gcaactgatt gattggatgg aagcggataa agtggcgggt ccgctgctgc - 2101 gtagcgcgct gccggctggc tggtttattg cggataaaag cggtgcgggc gaacgtggca - 2161 gccgtggcat tattgcggcg ctgggcccgg atggtaaacc gagccgtatt gtggtgattt - 2221 ataccaccgg cagccaggcg acgatggatg aacgtaaccg tcagattgcg gaaattggcg - 2281 cgagcctgat taaacattgg taaaccgata caattaaagg ctccttttgg agcctttttt - 2341 tttggacgac ccttgtcGGC TCG -// -LOCUS BS_x9x 2819 bp ds-DNA circular 13-SEP-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 60..1444 - /label="SEVA_pBR322-ROP" - misc_feature 1459..1563 - /label="SEVA_T1" - misc_feature 1573..1629 - /label="LMS" - misc_feature 1636..1738 - /label="SEVA_T0" - misc_feature 1765..2803 - /label="SEVA_Ap" - misc_feature join(2814..2819,1..51) - /label="LMP" -ORIGIN - 1 GGTAAGAACT CGCACTTCGT GGAAACACTA TTATCTGGTG GGTCTCTGTC Cggccggccc - 61 cgtagaaaag atcaaaggAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT - 121 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC - 181 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT - 241 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT - 301 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA - 361 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC - 421 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG - 481 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT - 541 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC - 601 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG - 661 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC - 721 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC - 781 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG - 841 CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC - 901 ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA - 961 TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC - 1021 GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC - 1081 GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG - 1141 CTGCGGTAAA GCTCATCAGC GTGGTCGTGC AGCGATTCAC AGATGTCTGC CTGTTCATCC - 1201 GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC - 1261 ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG - 1321 TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT - 1381 GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG - 1441 CGGGGGCGCG CCCAGCTGTC TAGGGCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG - 1501 ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTcgactg agcctttcgt tttatttgat - 1561 gcctttaatt aaGGCTCGGG AGACCTATCG GTAATAACAG TCCAATCTGG TGTAACTTCG - 1621 GAATCGTCCa ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat - 1681 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg - 1741 gtccccaata attacgattt aaattagtag cccgcctaat gagcgggctt ttttttaatt - 1801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac - 1861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg - 1921 tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc - 1981 tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg - 2041 atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga - 2101 gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac - 2161 aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg - 2221 aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga - 2281 gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg - 2341 cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga - 2401 acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc - 2461 tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt - 2521 ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt - 2581 ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg - 2641 gcccggatgg taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga - 2701 tggatgaacg taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa - 2761 ccgatacaat taaaggctcc ttttggagcc tttttttttg gacgaccctt gtcGGCTCG -// \ No newline at end of file diff --git a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/TS_ori_modules.gb b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/TS_ori_modules.gb index 76db42d..96fd627 100644 --- a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/TS_ori_modules.gb +++ b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/TS_ori_modules.gb @@ -1,4 +1,4 @@ -LOCUS BASIC_construct_BS_x7x_TS_CASc 2963 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_construct_BS_x7x_TS_CASc 2963 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC DNA Assembly of ['LMP', 'BS_x7x_TS_CASc', 'LMS', 'BS_1xx']. ACCESSION BS_x7x_TS_CASc VERSION BS_x7x_TS_CASc @@ -73,7 +73,7 @@ ORIGIN 2881 ttggcgcgag cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc 2941 tttttttttg gacgaccctt gtc // -LOCUS BASIC_construct_BS_x7x_TS_PKD46 2963 bp DNA circular UNK 09-SEP-2021 +LOCUS BASIC_construct_BS_x7x_TS_PKD46 2963 bp DNA circular UNK 03-NOV-2021 DEFINITION BASIC DNA Assembly of ['LMP', 'BS_x7x_TS_PKD46', 'LMS', 'BS_1xx']. ACCESSION BS_x7x_TS_PKD46 VERSION BS_x7x_TS_PKD46 diff --git a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/mScarlet_cs_cassettes.gb b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/mScarlet_cs_cassettes.gb index 8f0db87..fedd2e6 100644 --- a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/mScarlet_cs_cassettes.gb +++ b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/mScarlet_cs_cassettes.gb @@ -1,389 +1,4 @@ -LOCUS BS_x5x 5108 bp ds-DNA circular 29-JUL-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 1..57 - /label="LMP" - misc_feature 66..3739 - /label="SEVA_RSF101" - misc_feature 3754..3858 - /label="SEVA_T1" - misc_feature 3868..3924 - /label="LMS" - misc_feature 3931..4033 - /label="SEVA_T0" - misc_feature 4060..5098 - /label="SEVA_Ap" -ORIGIN - 1 GGCTCGGGTA AGAACTCGCA CTTCGTGGAA ACACTATTAT CTGGTGGGTC TCTGTCCggc - 61 cggcctcagc ctgccgcctt gggccgggtg atgtcgtact tgcccgccgc gaactcggtt - 121 accgtccagc ccagcgcgac cagctccggc aacgcctcgc gcacccgctg gcggcgcttg - 181 cgcatggtcg aaccactggc ctctgacggc cagacatagc cgcacaaggt atctatggaa - 241 gccttgccgg ttttgccggg gtcgatccag ccacacagcc gctggtgcag caggcgggcg - 301 gtttcgctgt ccagcgcccg cacctcgtcc atgctgatgc gcacatgctg gccgccaccc - 361 atgacggcct gcgcgatcaa ggggttcagg gccacgtaca ggcgcccgtc cgcctcgtcg - 421 ctggcgtact ccgacagcag ccgaaacccc tgccgcttgc ggccattctg ggcgatgatg - 481 gataccttcc aaaggcgctc gatgcagtcc tgtatgtgct tgagcgcccc accactatcg - 541 acctctgccc cgatttcctt tgccagcgcc cgatagctac ctttgaccac atggcattca - 601 gcggtgacgg cctcccactt gggttccagg aacagccgga gctgccgtcc gccttcggtc - 661 ttgggttccg ggccaagcac taggccatta ggcccagcca tggccaccag cccttgcagg - 721 atgcgcagat catcagcgcc cagcggctcc gggccgctga actcgatccg cttgccgtcg - 781 ccgtagtcat acgtcacgtc cagcttgctg cgcttgcgct cgccccgctt gagggcacgg - 841 aacaggccgg gggccagaca gtgcgccggg tcgtgccgga cgtggctgag gctgtgcttg - 901 ttcttaggct tcaccacggg gcaccccctt gctcttgcgc tgcctctcca gcacggcggg - 961 cttgagcacc ccgccgtcat gccgcctgaa ccaccgatca gcgaacggtg cgccatagtt - 1021 ggccttgctc acaccgaagc ggacgaagaa ccggcgctgg tcgtcgtcca caccccattc - 1081 ctcggcctcg gcgctggtca tgctcgacag gtaggactgc cagcggatgt tatcgaccag - 1141 taccgagctg ccccggctgg cctgctgctg gtcgcctgcg cccatcatgg ccgcgccctt - 1201 gctggcatgg tgcaggaaca cgatagagca cccggtatcg gcggcgatgg cctccatgcg - 1261 accgatgacc tgggccatgg ggccgctggc gttttcttcc tcgatgtgga accggcgcag - 1321 cgtgtccagc accatcaggc ggcggccctc ggcggcgcgc ttgaggccgt cgaaccactc - 1381 cggggccatg atgttgggca ggctgccgat cagcggctgg atcagcaggc cgtcagccac - 1441 ggcttgccgt tcctcggcgc tgaggtgcgc cccaagggcg tgcaggcggt gatgaatggc - 1501 ggtgggcggg tcttcggcgg gcaggtagat caccgggccg gtgggcagtt cgcccacctc - 1561 cagcagatcc ggcccgcctg caatctgtgc ggccagttgc agggccagca tggatttacc - 1621 ggcaccaccg ggcgacacca gcgccccgac cgtaccggcc accatgttgg gcaaaacgta - 1681 gtccagcggt ggcggcgctg ctgcgaacgc ctccagaata ttgataggct tatgggtagc - 1741 cattgattgc ctcctttgca ggcagttggt ggttaggcgc tggcggggtc actacccccg - 1801 ccctgcgccg ctctgagttc ttccaggcac tcgcgcagcg cctcgtattc gtcgtcggtc - 1861 agccagaact tgcgctgacg catccctttg gccttcatgc gctcggcata tcgcgcttgg - 1921 cgtacagcgt cagggctggc cagcaggtcg ccggtctgct tgtccttttg gtctttcata - 1981 tcagtcaccg agaaacttgc cggggccgaa aggcttgtct tcgcggaaca aggacaaggt - 2041 gcagccgtca aggttaaggc tggccatatc agcgactgaa aagcggccag cctcggcctt - 2101 gtttgacgta taaccaaagc caccgggcaa ccaatagccc ttgtcacttt tgatcaggta - 2161 gaccgaccct gaagcgcttt tttcgtattc cataaaaccc ccttctgtgc gtgagtactc - 2221 atagtataac aggcgtgagt accaacgcaa gcactacatg ctgaaatctg gcccgcccct - 2281 gtccatgcct cgctggcggg gtgccggtgc ccgtgccagc tcggcccgcg caagctggac - 2341 gctgggcaga cccatgacct tgctgacggt gcgctcgatg taatccgctt cgtggccggg - 2401 cttgcgctct gccagcgctg ggctggcctc ggccatggcc ttgccgattt cctcggcact - 2461 gcggccccgg ctggccagct tctgcgcggc gataaagtcg cacttgctga ggtcatcacc - 2521 gaagcgcttg accagcccgg ccatctcgct gcggtactcg tccagcgccg tgcgccggtg - 2581 gcggctaagc tgccgctcgg gcagttcgag gctggccagc ctgcgggcct tctcctgctg - 2641 ccgctgggcc tgctcgatct gctggccagc ctgctgcacc agcgccgggc cagcggtggc - 2701 ggtcttgccc ttggattcac gcagcagcac ccacggctga taaccggcgc gggtggtgtg - 2761 cttgtccttg cggttggtga agcccgccaa gcggccatag tggcggctgt cggcgctggc - 2821 cgggtcggcg tcgtactcgc tggccagcgt ccgggcaatc tgcccccgaa gttcaccgcc - 2881 tgcggcgtcg gccaccttga cccatgcctg atagttcttc gggctggttt ccactaccag - 2941 ggcaggctcc cggccctcgg ctttcatgtc atccaggtca aactcgctga ggtcgtccac - 3001 cagcaccaga ccatgccgct cctgctcggc gggcctgata tacacgtcat tgccctgggc - 3061 attcatccgc ttgagccatg gcgtgttctg gagcacttcg gcggctgacc attcccggtt - 3121 catcatctgg ccggtggtgg cgtccctgac gccgatatcg aagcgctcac agcccatggc - 3181 cttgagctgt cggcctatgg cctgcaaagt cctgtcgttc ttcatcgggc caccaagcga - 3241 ttcccacaca ttatacgagc cggaagcata aagtgtaaag cctagatccg aaggatgagc - 3301 cgggctgaat gatcgaccga gacaggccct gcggggctgc acacgcgccc ccacccttcg - 3361 ggtaggggga aaggccgcta aagcggctaa aagcgctcca gcgtatttct gcggggtttg - 3421 gtgtggggtt tagcgggctt tgcccgcctt tccccctgcc gcgcagcggt ggggcggtgt - 3481 gtagcctagc gcagcgaata gaccagctat ccggcctctg gccgggcata ttgggcaagg - 3541 gcagcagcgc cccacaaggg cgctgataac cgcgcctagt ggattattct tagataatca - 3601 tggatggatt tttccaacac cccgccagcc cccgcccctg ctgggtttgc aggtttgggg - 3661 gcgtgacagt tattgcaggg gttcgtgaca gttattgcag gggggcgtga cagttattgc - 3721 aggggttcgt gacagttagg gcgcgcccag ctgtctaggg cggcggattt gtcctactca - 3781 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct - 3841 ttcgttttat ttgatgcctt taattaaGGC TCGGGAGACC TATCGGTAAT AACAGTCCAA - 3901 TCTGGTGTAA CTTCGGAATC GTCCactagt cttggactcc tgttgataga tccagtaatg - 3961 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt - 4021 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg - 4081 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc - 4141 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca - 4201 ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg - 4261 cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg - 4321 gctatattga actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac - 4381 gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg - 4441 atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat - 4501 atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg - 4561 cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc - 4621 cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt - 4681 gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag - 4741 caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc - 4801 agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc - 4861 tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca - 4921 ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg - 4981 gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga - 5041 ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga - 5101 cccttgtc -// -LOCUS BS_x6x 2166 bp ds-DNA circular 05-AUG-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 60..791 - /label="SEVA_p15A" - misc_feature 806..910 - /label="SEVA_T1" - misc_feature 920..976 - /label="LMS" - misc_feature 983..1085 - /label="SEVA_T0" - misc_feature 1112..2150 - /label="SEVA_Ap" - misc_feature join(2161..2166,1..51) - /label="LMP" -ORIGIN - 1 GGTAAGAACT CGCACTTCGT GGAAACACTA TTATCTGGTG GGTCTCTGTC Cggccggccc - 61 tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta - 121 atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga - 181 gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc - 241 ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc - 301 agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt - 361 accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga - 421 gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca - 481 gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag - 541 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc - 601 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc - 661 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta - 721 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc - 781 ggaatatatc cGGCGCGCCC AGCTGTCTAG GGCGGCGGAT TTGTCCTACT CAGGAGAGCG - 841 TTCACCGACA AACAACAGAT AAAACGAAAG GCCCAGTCTT Tcgactgagc ctttcgtttt - 901 atttgatgcc tttaattaaG GCTCGGGAGA CCTATCGGTA ATAACAGTCC AATCTGGTGT - 961 AACTTCGGAA TCGTCCacta gtcttggact cctgttgata gatccagtaa tgacctcaga - 1021 actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta ttggtgagaa - 1081 tccaggggtc cccaataatt acgatttaaa ttagtagccc gcctaatgag cgggcttttt - 1141 tttaattccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga - 1201 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag cattcagcat - 1261 tttcgtgtgg cgctgattcc gttttttgcg gcgttttgcc tgccggtgtt tgcgcatccg - 1321 gaaaccctgg tgaaagtgaa agatgcggaa gatcaactgg gtgcgcgcgt gggctatatt - 1381 gaactggatc tgaacagcgg caaaattctg gaatcttttc gtccggaaga acgttttccg - 1441 atgatgagca cctttaaagt gctgctgtgc ggtgcggttc tgagccgtgt ggatgcgggc - 1501 caggaacaac tgggccgtcg tattcattat agccagaacg atctggtgga atatagcccg - 1561 gtgaccgaaa aacatctgac cgatggcatg accgtgcgtg aactgtgcag cgcggcgatt - 1621 accatgagcg ataacaccgc ggcgaacctg ctgctgacga ccattggcgg tccgaaagaa - 1681 ctgaccgcgt ttctgcataa catgggcgat catgtgaccc gtctggatcg ttgggaaccg - 1741 gaactgaacg aagcgattcc gaacgatgaa cgtgatacca ccatgccggc agcaatggcg - 1801 accaccctgc gtaaactgct gacgggtgag ctgctgaccc tggcaagccg ccagcaactg - 1861 attgattgga tggaagcgga taaagtggcg ggtccgctgc tgcgtagcgc gctgccggct - 1921 ggctggttta ttgcggataa aagcggtgcg ggcgaacgtg gcagccgtgg cattattgcg - 1981 gcgctgggcc cggatggtaa accgagccgt attgtggtga tttataccac cggcagccag - 2041 gcgacgatgg atgaacgtaa ccgtcagatt gcggaaattg gcgcgagcct gattaaacat - 2101 tggtaaaccg atacaattaa aggctccttt tggagccttt ttttttggac gacccttgtc - 2161 GGCTCG -// -LOCUS BS_x7x 2894 bp ds-DNA circular 29-JUL-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 60..1519 - /label="SEVA_pSC101" - misc_feature 1534..1638 - /label="SEVA_T1" - misc_feature 1648..1704 - /label="LMS" - misc_feature 1711..1813 - /label="SEVA_T0" - misc_feature 1840..2878 - /label="SEVA_Ap" - misc_feature join(2889..2894,1..51) - /label="LMP" -ORIGIN - 1 GGTAAGAACT CGCACTTCGT GGAAACACTA TTATCTGGTG GGTCTCTGTC Cggccggcct - 61 cagatccttc cgTATTTAGC CAGTATGTTC TCTAGTGTGG TTCGTTGTTT TTGCGTGAGC - 121 CATGAGAACG AACCATTGAG ATCATACTTA CTTTGCATGT CACTCAAAAA TTTTGCCTCA - 181 AAACTGGTGA GCTGAATTTT TGCAGTTAAA GCATCGTGTA GTGTTTTTCT TAGTCCGTTA - 241 TGTAGGTAGG AATCTGATGT AATGGTTGTT GGTATTTTGT CACCATTCAT TTTTATCTGG - 301 TTGTTCTCAA GTTCGGTTAC GAGATCCATT TGTCTATCTA GTTCAACTTG GAAAATCAAC - 361 GTATCAGTCG GGCGGCCTCG CTTATCAACC ACCAATTTCA TATTGCTGTA AGTGTTTAAA - 421 TCTTTACTTA TTGGTTTCAA AACCCATTGG TTAAGCCTTT TAAACTCATG GTAGTTATTT - 481 TCAAGCATTA ACATGAACTT AAATTCATCA AGGCTAATCT CTATATTTGC CTTGTGAGTT - 541 TTCTTTTGTG TTAGTTCTTT TAATAACCAC TCATAAATCC TCATAGAGTA TTTGTTTTCA - 601 AAAGACTTAA CATGTTCCAG ATTATATTTT ATGAATTTTT TTAACTGGAA AAGATAAGGC - 661 AATATCTCTT CACTAAAAAC TAATTCTAAT TTTTCGCTTG AGAACTTGGC ATAGTTTGTC - 721 CACTGGAAAA TCTCAAAGCC TTTAACCAAA GGATTCCTGA TTTCCACAGT TCTCGTCATC - 781 AGCTCTCTGG TTGCTTTAGC TAATACACCA TAAGCATTTT CCCTACTGAT GTTCATCATC - 841 TGAGCGTATT GGTTATAAGT GAACGATACC GTCCGTTCTT TCCTTGTAGG GTTTTCAATC - 901 GTGGGGTTGA GTAGTGCCAC ACAGCATAAA ATTAGCTTGG TTTCATGCTC CGTTAAGTCA - 961 TAGCGACTAA TCGCTAGTTC ATTTGCTTTG AAAACAACTA ATTCAGACAT ACATCTCAAT - 1021 TGGTCTAGGT GATTTTAATC ACTATACCAA TTGAGATGGG CTAGTCAATG ATAATTACAT - 1081 GTCCTTTTCC TTTGAGTTGT GGGTATCTGT AAATTCTGCT AGACCTTTGC TGGAAAACTT - 1141 GTAAATTCTG CTAGACCCTC TGTAAATTCC GCTAGACCTT TGTGTGTTTT TTTTGTTTAT - 1201 ATTCAAGTGG TTATAATTTA TAGAATAAAG AAAGAATAAA AAAAGATAAA AAGAATAGAT - 1261 CCCAGCCCTG TGTATAACTC ACTACTTTAG TCAGTTCCGC AGTATTACAA AAGGATGTCG - 1321 CAAACGCTGT TTGCTCCTCT ACAAAACAGA CCTTAAAACC CTAAAGGCTT AAGTAGCACC - 1381 CTCGCAAGCT CGGGCAAATC GCTGAATATT CCTTTTGTCT CCGACCATCA GGCACCTGAG - 1441 TCGCTGTCTT TTTCGTGACA TTCAGTTCGC TGCGCTCACG GCTCTGGCAG TGAATGGGGG - 1501 TAAATGGCAC TACAGGCGCG GCGCGCCCAG CTGTCTAGGG CGGCGGATTT GTCCTACTCA - 1561 GGAGAGCGTT CACCGACAAA CAACAGATAA AACGAAAGGC CCAGTCTTTc gactgagcct - 1621 ttcgttttat ttgatgcctt taattaaGGC TCGGGAGACC TATCGGTAAT AACAGTCCAA - 1681 TCTGGTGTAA CTTCGGAATC GTCCactagt cttggactcc tgttgataga tccagtaatg - 1741 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt - 1801 ggtgagaatc caggggtccc caataattac gatttaaatt agtagcccgc ctaatgagcg - 1861 ggcttttttt taattcccct atttgtttat ttttctaaat acattcaaat atgtatccgc - 1921 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagca - 1981 ttcagcattt tcgtgtggcg ctgattccgt tttttgcggc gttttgcctg ccggtgtttg - 2041 cgcatccgga aaccctggtg aaagtgaaag atgcggaaga tcaactgggt gcgcgcgtgg - 2101 gctatattga actggatctg aacagcggca aaattctgga atcttttcgt ccggaagaac - 2161 gttttccgat gatgagcacc tttaaagtgc tgctgtgcgg tgcggttctg agccgtgtgg - 2221 atgcgggcca ggaacaactg ggccgtcgta ttcattatag ccagaacgat ctggtggaat - 2281 atagcccggt gaccgaaaaa catctgaccg atggcatgac cgtgcgtgaa ctgtgcagcg - 2341 cggcgattac catgagcgat aacaccgcgg cgaacctgct gctgacgacc attggcggtc - 2401 cgaaagaact gaccgcgttt ctgcataaca tgggcgatca tgtgacccgt ctggatcgtt - 2461 gggaaccgga actgaacgaa gcgattccga acgatgaacg tgataccacc atgccggcag - 2521 caatggcgac caccctgcgt aaactgctga cgggtgagct gctgaccctg gcaagccgcc - 2581 agcaactgat tgattggatg gaagcggata aagtggcggg tccgctgctg cgtagcgcgc - 2641 tgccggctgg ctggtttatt gcggataaaa gcggtgcggg cgaacgtggc agccgtggca - 2701 ttattgcggc gctgggcccg gatggtaaac cgagccgtat tgtggtgatt tataccaccg - 2761 gcagccaggc gacgatggat gaacgtaacc gtcagattgc ggaaattggc gcgagcctga - 2821 ttaaacattg gtaaaccgat acaattaaag gctccttttg gagccttttt ttttggacga - 2881 cccttgtcGG CTCG -// -LOCUS BS_x7x_TS_CASc 1701 bp ds-DNA linear 05-AUG-2021 -DEFINITION . -KEYWORDS "creator:Trial User" -FEATURES Location/Qualifiers - CDS complement(80..1030) - /label="Rep101(Ts)" - rep_origin 1078..1300 - /label="pSC101 ori" - misc_feature 1570..1674 - /label="SEVA_T1" -ORIGIN - 1 TCTGGTGGGT CTCTGTCCtg ttctactttt gtttgttagt cttgatgctt cactgataga - 61 tacaagagcc ataagaacct cagatccttc cgtatttagc cagtatgttc tctagtgtgg - 121 ttcgttgttt ttgcgtgagc catgagaacg aaccattgag atcatactta ctttgcatgt - 181 cactcaaaaa ttttgcctca aaactggtga gctgaatttt tgcagttaaa gcatcgtgta - 241 gtgtttttct tagtccgtta tgtaggtagg aatctgatgt aatggttgtt ggtattttgt - 301 caccattcat ttttatctgg ttgttctcaa gttcggttac gagatccatt tgtctatcta - 361 gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca - 421 tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt - 481 taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct - 541 ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc - 601 tcatagagta tttgttttca aaagacttaa catgttccag attatatttt atgaattttt - 661 ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg - 721 agaacttggc atagtttgtc cactggaaaa tctcaaagcc tttaaccaaa ggattcctga - 781 tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt - 841 ccctactgat gttcatcatc tgaacgtatt ggttataagt gaacgatacc gtccgttctt - 901 tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg - 961 tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta - 1021 attcagacat acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg - 1081 ctagtcaatg ataattacta gtccttttcc tttgagttgt gggtatctgt aaattctgct - 1141 agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt - 1201 tgtgtgtttt ttttgtttat attcaagtgg ttataattta tagaataaag aaagaataaa - 1261 aaaagataaa aagaatagat cccagccctg tgtataactc actactttag tcagttccgc - 1321 agtattacaa aaggatgtcg caaacgctgt ttgctcctct acaaaacaga ccttaaaacc - 1381 ctaaaggctt aagtagcacc ctcgcaagct cggttgcggc cgcaatcggg caaatcgctg - 1441 aatattcctt ttgtctccga ccatcaggca cctgagtcgc tgtctttttc gtgacattca - 1501 gttcgctgcg ctcacggctc tggcagtgaa tgggggtaaa tggcactaca ggcgcGGCGC - 1561 GCCCAGCTGT CTAGGGCGGC GGATTTGTCC TACTCAGGAG AGCGTTCACC GACAAACAAC - 1621 AGATAAAACG AAAGGCCCAG TCTTTcgact gagcctttcg ttttatttga tgcctttaat - 1681 taaGGCTCGG GAGACCTATC G -// -LOCUS BS_x8x 2363 bp ds-DNA circular 29-JUL-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 60..988 - /label="SEVA_pUC" - misc_feature 1003..1107 - /label="SEVA_T1" - misc_feature 1117..1173 - /label="LMS" - misc_feature 1180..1282 - /label="SEVA_T0" - misc_feature 1309..2347 - /label="SEVA_Ap" - misc_feature join(2358..2363,1..51) - /label="LMP" -ORIGIN - 1 GGTAAGAACT CGCACTTCGT GGAAACACTA TTATCTGGTG GGTCTCTGTC Cggccggccc - 61 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt - 121 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac - 181 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt - 241 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct - 301 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga - 361 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac - 421 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctttg - 481 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt - 541 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc - 601 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg - 661 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc - 721 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc - 781 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag - 841 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca - 901 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat - 961 taatgtgagt tagctcactc attaggcaGG CGCGCCCAGC TGTCTAGGGC GGCGGATTTG - 1021 TCCTACTCAG GAGAGCGTTC ACCGACAAAC AACAGATAAA ACGAAAGGCC CAGTCTTTcg - 1081 actgagcctt tcgttttatt tgatgccttt aattaaGGCT CGGGAGACCT ATCGGTAATA - 1141 ACAGTCCAAT CTGGTGTAAC TTCGGAATCG TCCactagtc ttggactcct gttgatagat - 1201 ccagtaatga cctcagaact ccatctggat ttgttcagaa cgctcggttg ccgccgggcg - 1261 ttttttattg gtgagaatcc aggggtcccc aataattacg atttaaatta gtagcccgcc - 1321 taatgagcgg gctttttttt aattccccta tttgtttatt tttctaaata cattcaaata - 1381 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga - 1441 gtatgagcat tcagcatttt cgtgtggcgc tgattccgtt ttttgcggcg ttttgcctgc - 1501 cggtgtttgc gcatccggaa accctggtga aagtgaaaga tgcggaagat caactgggtg - 1561 cgcgcgtggg ctatattgaa ctggatctga acagcggcaa aattctggaa tcttttcgtc - 1621 cggaagaacg ttttccgatg atgagcacct ttaaagtgct gctgtgcggt gcggttctga - 1681 gccgtgtgga tgcgggccag gaacaactgg gccgtcgtat tcattatagc cagaacgatc - 1741 tggtggaata tagcccggtg accgaaaaac atctgaccga tggcatgacc gtgcgtgaac - 1801 tgtgcagcgc ggcgattacc atgagcgata acaccgcggc gaacctgctg ctgacgacca - 1861 ttggcggtcc gaaagaactg accgcgtttc tgcataacat gggcgatcat gtgacccgtc - 1921 tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa cgatgaacgt gataccacca - 1981 tgccggcagc aatggcgacc accctgcgta aactgctgac gggtgagctg ctgaccctgg - 2041 caagccgcca gcaactgatt gattggatgg aagcggataa agtggcgggt ccgctgctgc - 2101 gtagcgcgct gccggctggc tggtttattg cggataaaag cggtgcgggc gaacgtggca - 2161 gccgtggcat tattgcggcg ctgggcccgg atggtaaacc gagccgtatt gtggtgattt - 2221 ataccaccgg cagccaggcg acgatggatg aacgtaaccg tcagattgcg gaaattggcg - 2281 cgagcctgat taaacattgg taaaccgata caattaaagg ctccttttgg agcctttttt - 2341 tttggacgac ccttgtcGGC TCG -// -LOCUS BS_x9x 2819 bp ds-DNA circular 29-JUL-2021 -DEFINITION . -FEATURES Location/Qualifiers - misc_feature 60..1444 - /label="SEVA_pBR322-ROP" - misc_feature 1459..1563 - /label="SEVA_T1" - misc_feature 1573..1629 - /label="LMS" - misc_feature 1636..1738 - /label="SEVA_T0" - misc_feature 1765..2803 - /label="SEVA_Ap" - misc_feature join(2814..2819,1..51) - /label="LMP" -ORIGIN - 1 GGTAAGAACT CGCACTTCGT GGAAACACTA TTATCTGGTG GGTCTCTGTC Cggccggccc - 61 cgtagaaaag atcaaaggAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT - 121 GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC - 181 TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT - 241 GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT - 301 GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA - 361 CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC - 421 ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG - 481 AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT - 541 CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC - 601 TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG - 661 GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC - 721 TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC - 781 CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG - 841 CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC - 901 ACACCGCATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA - 961 TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC - 1021 GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC - 1081 GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG - 1141 CTGCGGTAAA GCTCATCAGC GTGGTCGTGC AGCGATTCAC AGATGTCTGC CTGTTCATCC - 1201 GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC - 1261 ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG - 1321 TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT - 1381 GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG - 1441 CGGGGGCGCG CCCAGCTGTC TAGGGCGGCG GATTTGTCCT ACTCAGGAGA GCGTTCACCG - 1501 ACAAACAACA GATAAAACGA AAGGCCCAGT CTTTcgactg agcctttcgt tttatttgat - 1561 gcctttaatt aaGGCTCGGG AGACCTATCG GTAATAACAG TCCAATCTGG TGTAACTTCG - 1621 GAATCGTCCa ctagtcttgg actcctgttg atagatccag taatgacctc agaactccat - 1681 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaggg - 1741 gtccccaata attacgattt aaattagtag cccgcctaat gagcgggctt ttttttaatt - 1801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac - 1861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagcattcag cattttcgtg - 1921 tggcgctgat tccgtttttt gcggcgtttt gcctgccggt gtttgcgcat ccggaaaccc - 1981 tggtgaaagt gaaagatgcg gaagatcaac tgggtgcgcg cgtgggctat attgaactgg - 2041 atctgaacag cggcaaaatt ctggaatctt ttcgtccgga agaacgtttt ccgatgatga - 2101 gcacctttaa agtgctgctg tgcggtgcgg ttctgagccg tgtggatgcg ggccaggaac - 2161 aactgggccg tcgtattcat tatagccaga acgatctggt ggaatatagc ccggtgaccg - 2221 aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg cagcgcggcg attaccatga - 2281 gcgataacac cgcggcgaac ctgctgctga cgaccattgg cggtccgaaa gaactgaccg - 2341 cgtttctgca taacatgggc gatcatgtga cccgtctgga tcgttgggaa ccggaactga - 2401 acgaagcgat tccgaacgat gaacgtgata ccaccatgcc ggcagcaatg gcgaccaccc - 2461 tgcgtaaact gctgacgggt gagctgctga ccctggcaag ccgccagcaa ctgattgatt - 2521 ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag cgcgctgccg gctggctggt - 2581 ttattgcgga taaaagcggt gcgggcgaac gtggcagccg tggcattatt gcggcgctgg - 2641 gcccggatgg taaaccgagc cgtattgtgg tgatttatac caccggcagc caggcgacga - 2701 tggatgaacg taaccgtcag attgcggaaa ttggcgcgag cctgattaaa cattggtaaa - 2761 ccgatacaat taaaggctcc ttttggagcc tttttttttg gacgaccctt gtcGGCTCG -// -LOCUS B405_J23106-RBS34-mScarl 806 bp ds-DNA linear 26-AUG-2021 +LOCUS B405_J23106-RBS34-mScarl 806 bp ds-DNA linear 26-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 19..53 @@ -408,7 +23,7 @@ ORIGIN 721 AATATGAGCG TAGCGAGGGT CGCCACAGCA CCGGCGGCAT GGACGAACTG TATAAGGGAT 781 CCtaataaGG CTCGGGAGAC CTATCG // -LOCUS B407_J23119-RBS34-mScarl 806 bp ds-DNA linear 26-AUG-2021 +LOCUS B407_J23119-RBS34-mScarl 806 bp ds-DNA linear 15-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 19..53 @@ -433,7 +48,7 @@ ORIGIN 721 AATATGAGCG TAGCGAGGGT CGCCACAGCA CCGGCGGCAT GGACGAACTG TATAAGGGAT 781 CCtaataaGG CTCGGGAGAC CTATCG // -LOCUS B408_J23119-RBS-A12-mSc 1002 bp ds-DNA linear 26-AUG-2021 +LOCUS B408_J23119-RBS-A12-mSc 1002 bp ds-DNA linear 15-OCT-2021 DEFINITION . FEATURES Location/Qualifiers misc_feature 111..145 diff --git a/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/oris.gb b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/oris.gb new file mode 100644 index 0000000..2553fbd --- /dev/null +++ b/sequences/genbank_files/BASIC_SEVA_collection/initial_mods/oris.gb @@ -0,0 +1,360 @@ +LOCUS BS_x5ax 5108 bp ds-DNA circular 15-OCT-2021 +DEFINITION . +FEATURES Location/Qualifiers + misc_feature 614..670 + /label="LMP" + misc_feature 682..4352 + /label="SEVA_RSF1010(x5ax)" + misc_feature 4367..4471 + /label="SEVA_T1" + misc_feature 4481..4537 + /label="LMS" + misc_feature 4544..4646 + /label="SEVA_T0" + misc_feature join(4673..5108,1..603) + /label="SEVA_Ap" +ORIGIN + 1 ggaatatagc ccggtgaccg aaaaacatct gaccgatggc atgaccgtgc gtgaactgtg + 61 cagcgcggcg attaccatga gcgataacac cgcggcgaac ctgctgctga cgaccattgg + 121 cggtccgaaa gaactgaccg cgtttctgca taacatgggc gatcatgtga cccgtctgga + 181 tcgttgggaa ccggaactga acgaagcgat tccgaacgat gaacgtgata ccaccatgcc + 241 ggcagcaatg gcgaccaccc tgcgtaaact gctgacgggt gagctgctga ccctggcaag + 301 ccgccagcaa ctgattgatt ggatggaagc ggataaagtg gcgggtccgc tgctgcgtag + 361 cgcgctgccg gctggctggt ttattgcgga taaaagcggt gcgggcgaac gtggcagccg + 421 tggcattatt gcggcgctgg gcccggatgg taaaccgagc cgtattgtgg tgatttatac + 481 caccggcagc caggcgacga tggatgaacg taaccgtcag attgcggaaa ttggcgcgag + 541 cctgattaaa cattggtaaa ccgatacaat taaaggctcc ttttggagcc tttttttttg + 601 gacgaccctt gtcGGCTCGG GTAAGAACTC GCACTTCGTG GAAACACTAT TATCTGGTGG + 661 GTCTCTGTCC GTAggccggc ctcagcctgc cgccttgggc cgggtgatgt cgtacttgcc + 721 cgccgcgaac tcggttaccg tccagcccag cgcgaccagc tccggcaacg cctcgcgcac + 781 ccgctggcgg cgcttgcgca tggtcgaacc actggcctct gacggccaga catagccgca + 841 caaggtatct atggaagcct tgccggtttt gccggggtcg atccagccac acagccgctg + 901 gtgcagcagg cgggcggttt cgctgtccag cgcccgcacc tcgtccatgc tgatgcgcac + 961 atgctggccg ccacccatga cggcctgcgc gatcaagggg ttcagggcca cgtacaggcg + 1021 cccgtccgcc tcgtcgctgg cgtactccga cagcagccga aacccctgcc gcttgcggcc + 1081 attctgggcg atgatggata ccttccaaag gcgctcgatg cagtcctgta tgtgcttgag + 1141 cgccccacca ctatcgacct ctgccccgat ttcctttgcc agcgcccgat agctaccttt + 1201 gaccaccatg gcatcagcgg tgacggcctc ccacttgggt tccaggaaca gccggagctg + 1261 ccgtccgcct tcggtcttgg gttccgggcc aagcactagg ccattaggcc cagccatggc + 1321 caccagccct tgcaggatgc gcagatcatc agcgcccagc ggctccgggc cgctgaactc + 1381 gatccgcttg ccgtcgccgt agtcatacgt cacgtccagc ttgctgcgct tgcgctcgcc + 1441 ccgcttgagg gcacggaaca ggccgggggc cagacagtgc gccgggtcgt gccggacgtg + 1501 gctgaggctg tgcttgttct taggcttcac cacggggcac ccccttgctc ttgcgctgcc + 1561 tctccagcac ggcgggcttg agcaccccgc cgtcatgccg cctgaaccac cgatcagcga + 1621 acggtgcgcc atagttggcc ttgctcacac cgaagcggac gaagaaccgg cgctggtcgt + 1681 cgtccacacc ccattcctcg gcctcggcgc tggtcatgct cgacaggtag gactgccagc + 1741 ggatgttatc gaccagtacc gagctgcccc ggctggcctg ctgctggtcg cctgcgccca + 1801 tcatggccgc gcccttgctg gcatggtgca ggaacacgat agagcacccg gtatcggcgg + 1861 cgatggcctc catgcgaccg atgacctggg ccatggggcc gctggcgttt tcttcctcga + 1921 tgtggaaccg gcgcagcgtg tccagcacca tcaggcggcg gccctcggcg gcgcgcttga + 1981 ggccgtcgaa ccactccggg gccatgatgt tgggcaggct gccgatcagc ggctggatca + 2041 gcaggccgtc agccacggct tgccgttcct cggcgctgag gtgcgcccca agggcgtgca + 2101 ggcggtgatg aatggcggtg ggcgggtctt cggcgggcag gtagatcacc gggccggtgg + 2161 gcagttcgcc cacctccagc agatccggcc cgcctgcaat ctgtgcggcc agttgcaggg + 2221 ccagcatgga tttaccggca ccaccgggcg acaccagcgc cccgaccgta ccggccacca + 2281 tgttgggcaa aacgtagtcc agcggtggcg gcgctgctgc gaacgcctcc agaatattga + 2341 taggcttatg ggtagccatt gattgcctcc tttgcaggca gttggtggtt aggcgctggc + 2401 ggggtcacta cccccgccct gcgccgctct gagttcttcc aggcactcgc gcagcgcctc + 2461 gtattcgtcg tcggtcagcc agaacttgcg ctgacgcatc cctttggcct tcatgcgctc + 2521 ggcatatcgc gcttggcgta cagcgtcagg gctggccagc aggtcgccgg tctgcttgtc + 2581 cttttggtct ttcatatcag tcaccgagaa acttgccggg gccgaaaggc ttgtcttcgc + 2641 ggaacaagga caaggtgcag ccgtcaaggt taaggctggc catatcagcg actgaaaagc + 2701 ggccagcctc ggccttgttt gacgtataac caaagccacc gggcaaccaa tagcccttgt + 2761 cacttttgat caggtagacc gaccctgaag cgcttttttc gtattccata aaaccccctt + 2821 ctgtgcgtga gtactcatag tataacaggc gtgagtacca acgcaagcac tacatgctga + 2881 aatctggccc gcccctgtcc atgcctcgct ggcggggtgc cggtgcccgt gccagctcgg + 2941 cccgcgcaag ctggacgctg ggcagaccca tgaccttgct gacggtgcgc tcgatgtaat + 3001 ccgcttcgtg gccgggcttg cgctctgcca gcgctgggct ggcctcggcc atggccttgc + 3061 cgatttcctc ggcactgcgg ccccggctgg ccagcttctg cgcggcgata aagtcgcact + 3121 tgctgaggtc atcaccgaag cgcttgacca gcccggccat ctcgctgcgg tactcgtcca + 3181 gcgccgtgcg ccggtggcgg ctaagctgcc gctcgggcag ttcgaggctg gccagcctgc + 3241 gggccttctc ctgctgccgc tgggcctgct cgatctgctg gccagcctgc tgcaccagcg + 3301 ccgggccagc ggtggcggtc ttgcccttgg attcacgcag cagcacccac ggctgataac + 3361 cggcgcgggt ggtgtgcttg tccttgcggt tggtgaagcc cgccaagcgg ccatagtggc + 3421 ggctgtcggc gctggccggg tcggcgtcgt actcgctggc cagcgtccgg gcaatctgcc + 3481 cccgaagttc accgcctgcg gcgtcggcca ccttgaccca tgcctgatag ttcttcgggc + 3541 tggtttccac taccagggca ggctcccggc cctcggcttt catgtcatcc aggtcaaact + 3601 cgctgaggtc gtccaccagc accagaccat gccgctcctg ctcggcgggc ctgatataca + 3661 cgtcattgcc ctgggcattc atccgcttga gccatggcgt gttctggagc acttcggcgg + 3721 ctgaccattc ccggttcatc atctggccgg tggtggcgtc cctgacgccg atatcgaagc + 3781 gctcacagcc catggccttg agctgtcggc ctatggcctg caaagtcctg tcgttcttca + 3841 tcgggccacc aagcgattcc cacacattat acgagccgga agcataaagt gtagctagat + 3901 ccgaaggatg agccgggctg aatgatcgac cgagacaggc cctgcggggc tgcacacgcg + 3961 cccccaccct tcgggtaggg ggaaaggccg ctaaagcggc taaaagcgct ccagcgtatt + 4021 tctgcggggt ttggtgtggg gtttagcggg ctttgcccgc ctttccccct gccgcgcagc + 4081 ggtggggcgg tgtgtagcct agcgcagcga atagaccagc tatccggcct ctggccgggc + 4141 atattgggca agggcagcag cgccccacaa gggcgctgat aaccgcgcct agtggattat + 4201 tcttagataa tcatggatgg atttttccaa caccccgcca gcccccgccc ctgctgggtt + 4261 tgcaggtttg ggggcgtgac agttattgca ggggttcgtg acagttattg caggggggcg + 4321 tgacagttat tgcaggggtt cgtgacagtt agggcgcgcc cagctgtcta gggcggcgga + 4381 tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct + 4441 ttcgactgag cctttcgttt tatttgatgc ctttaattaa GGCTCGGGAG ACCTATCGGT + 4501 AATAACAGTC CAATCTGGTG TAACTTCGGA ATCGTCCact agtcttggac tcctgttgat + 4561 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg + 4621 ggcgtttttt attggtgaga atccaggggt ccccaataat tacgatttaa attagtagcc + 4681 cgcctaatga gcgggctttt ttttaattcc cctatttgtt tatttttcta aatacattca + 4741 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg + 4801 aagagtatga gcattcagca ttttcgtgtg gcgctgattc cgttttttgc ggcgttttgc + 4861 ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga aagatgcgga agatcaactg + 4921 ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg gcaaaattct ggaatctttt + 4981 cgtccggaag aacgttttcc gatgatgagc acctttaaag tgctgctgtg cggtgcggtt + 5041 ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc gtattcatta tagccagaac + 5101 gatctggt +// +LOCUS BS_x6x 2166 bp ds-DNA circular 15-OCT-2021 +DEFINITION . +FEATURES Location/Qualifiers + misc_feature 1..57 + /label="LMP" + misc_feature 66..797 + /label="SEVA_p15A" + misc_feature 812..916 + /label="SEVA_T1" + misc_feature 926..982 + /label="LMS" + misc_feature 989..1091 + /label="SEVA_T0" + misc_feature 1118..2156 + /label="SEVA_Ap" +ORIGIN + 1 GGCTCGGGTA AGAACTCGCA CTTCGTGGAA ACACTATTAT CTGGTGGGTC TCTGTCCggc + 61 cggccctaga aatattttat ctgattaata agatgatctt cttgagatcg ttttggtctg + 121 cgcgtaatct cttgctctga aaacgaaaaa accgccttgc agggcggttt ttcgaaggtt + 181 ctctgagcta ccaactcttt gaaccgaggt aactggcttg gaggagcgca gtcaccaaaa + 241 cttgtccttt cagtttagcc ttaaccggcg catgacttca agactaactc ctctaaatca + 301 attaccagtg gctgctgcca gtggtgcttt tgcatgtctt tccgggttgg actcaagacg + 361 atagttaccg gataaggcgc agcggtcgga ctgaacgggg ggttcgtgca tacagtccag + 421 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagac aaacgcggcc + 481 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc + 541 cgccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg + 601 agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg + 661 cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg + 721 ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga + 781 ggaagcggaa tatatccGGC GCGCCCAGCT GTCTAGGGCG GCGGATTTGT CCTACTCAGG + 841 AGAGCGTTCA CCGACAAACA ACAGATAAAA CGAAAGGCCC AGTCTTTcga ctgagccttt + 901 cgttttattt gatgccttta attaaGGCTC GGGAGACCTA TCGGTAATAA CAGTCCAATC + 961 TGGTGTAACT TCGGAATCGT CCactagtct tggactcctg ttgatagatc cagtaatgac + 1021 ctcagaactc catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg + 1081 tgagaatcca ggggtcccca ataattacga tttaaattag tagcccgcct aatgagcggg + 1141 ctttttttta attcccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc + 1201 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagcatt + 1261 cagcattttc gtgtggcgct gattccgttt tttgcggcgt tttgcctgcc ggtgtttgcg + 1321 catccggaaa ccctggtgaa agtgaaagat gcggaagatc aactgggtgc gcgcgtgggc + 1381 tatattgaac tggatctgaa cagcggcaaa attctggaat cttttcgtcc ggaagaacgt + 1441 tttccgatga tgagcacctt taaagtgctg ctgtgcggtg cggttctgag ccgtgtggat + 1501 gcgggccagg aacaactggg ccgtcgtatt cattatagcc agaacgatct ggtggaatat + 1561 agcccggtga ccgaaaaaca tctgaccgat ggcatgaccg tgcgtgaact gtgcagcgcg + 1621 gcgattacca tgagcgataa caccgcggcg aacctgctgc tgacgaccat tggcggtccg + 1681 aaagaactga ccgcgtttct gcataacatg ggcgatcatg tgacccgtct ggatcgttgg + 1741 gaaccggaac tgaacgaagc gattccgaac gatgaacgtg ataccaccat gccggcagca + 1801 atggcgacca ccctgcgtaa actgctgacg ggtgagctgc tgaccctggc aagccgccag + 1861 caactgattg attggatgga agcggataaa gtggcgggtc cgctgctgcg tagcgcgctg + 1921 ccggctggct ggtttattgc ggataaaagc ggtgcgggcg aacgtggcag ccgtggcatt + 1981 attgcggcgc tgggcccgga tggtaaaccg agccgtattg tggtgattta taccaccggc + 2041 agccaggcga cgatggatga acgtaaccgt cagattgcgg aaattggcgc gagcctgatt + 2101 aaacattggt aaaccgatac aattaaaggc tccttttgga gccttttttt ttggacgacc + 2161 cttgtc +// +LOCUS BS_x7x 2894 bp ds-DNA circular 15-OCT-2021 +DEFINITION . +FEATURES Location/Qualifiers + misc_feature 618..674 + /label="LMP" + misc_feature 683..2142 + /label="SEVA_pSC101" + misc_feature 2157..2261 + /label="SEVA_T1" + misc_feature 2271..2327 + /label="LMS" + misc_feature 2334..2436 + /label="SEVA_T0" + misc_feature join(2463..2894,1..607) + /label="SEVA_Ap" +ORIGIN + 1 tggtggaata tagcccggtg accgaaaaac atctgaccga tggcatgacc gtgcgtgaac + 61 tgtgcagcgc ggcgattacc atgagcgata acaccgcggc gaacctgctg ctgacgacca + 121 ttggcggtcc gaaagaactg accgcgtttc tgcataacat gggcgatcat gtgacccgtc + 181 tggatcgttg ggaaccggaa ctgaacgaag cgattccgaa cgatgaacgt gataccacca + 241 tgccggcagc aatggcgacc accctgcgta aactgctgac gggtgagctg ctgaccctgg + 301 caagccgcca gcaactgatt gattggatgg aagcggataa agtggcgggt ccgctgctgc + 361 gtagcgcgct gccggctggc tggtttattg cggataaaag cggtgcgggc gaacgtggca + 421 gccgtggcat tattgcggcg ctgggcccgg atggtaaacc gagccgtatt gtggtgattt + 481 ataccaccgg cagccaggcg acgatggatg aacgtaaccg tcagattgcg gaaattggcg + 541 cgagcctgat taaacattgg taaaccgata caattaaagg ctccttttgg agcctttttt + 601 tttggacgac ccttgtcGGC TCGGGTAAGA ACTCGCACTT CGTGGAAACA CTATTATCTG + 661 GTGGGTCTCT GTCCggccgg cctcagatcc ttccgTATTT AGCCAGTATG TTCTCTAGTG + 721 TGGTTCGTTG TTTTTGCGTG AGCCATGAGA ACGAACCATT GAGATCATAC TTACTTTGCA + 781 TGTCACTCAA AAATTTTGCC TCAAAACTGG TGAGCTGAAT TTTTGCAGTT AAAGCATCGT + 841 GTAGTGTTTT TCTTAGTCCG TTATGTAGGT AGGAATCTGA TGTAATGGTT GTTGGTATTT + 901 TGTCACCATT CATTTTTATC TGGTTGTTCT CAAGTTCGGT TACGAGATCC ATTTGTCTAT + 961 CTAGTTCAAC TTGGAAAATC AACGTATCAG TCGGGCGGCC TCGCTTATCA ACCACCAATT + 1021 TCATATTGCT GTAAGTGTTT AAATCTTTAC TTATTGGTTT CAAAACCCAT TGGTTAAGCC + 1081 TTTTAAACTC ATGGTAGTTA TTTTCAAGCA TTAACATGAA CTTAAATTCA TCAAGGCTAA + 1141 TCTCTATATT TGCCTTGTGA GTTTTCTTTT GTGTTAGTTC TTTTAATAAC CACTCATAAA + 1201 TCCTCATAGA GTATTTGTTT TCAAAAGACT TAACATGTTC CAGATTATAT TTTATGAATT + 1261 TTTTTAACTG GAAAAGATAA GGCAATATCT CTTCACTAAA AACTAATTCT AATTTTTCGC + 1321 TTGAGAACTT GGCATAGTTT GTCCACTGGA AAATCTCAAA GCCTTTAACC AAAGGATTCC + 1381 TGATTTCCAC AGTTCTCGTC ATCAGCTCTC TGGTTGCTTT AGCTAATACA CCATAAGCAT + 1441 TTTCCCTACT GATGTTCATC ATCTGAGCGT ATTGGTTATA AGTGAACGAT ACCGTCCGTT + 1501 CTTTCCTTGT AGGGTTTTCA ATCGTGGGGT TGAGTAGTGC CACACAGCAT AAAATTAGCT + 1561 TGGTTTCATG CTCCGTTAAG TCATAGCGAC TAATCGCTAG TTCATTTGCT TTGAAAACAA + 1621 CTAATTCAGA CATACATCTC AATTGGTCTA GGTGATTTTA ATCACTATAC CAATTGAGAT + 1681 GGGCTAGTCA ATGATAATTA CATGTCCTTT TCCTTTGAGT TGTGGGTATC TGTAAATTCT + 1741 GCTAGACCTT TGCTGGAAAA CTTGTAAATT CTGCTAGACC CTCTGTAAAT TCCGCTAGAC + 1801 CTTTGTGTGT TTTTTTTGTT TATATTCAAG TGGTTATAAT TTATAGAATA AAGAAAGAAT + 1861 AAAAAAAGAT AAAAAGAATA GATCCCAGCC CTGTGTATAA CTCACTACTT TAGTCAGTTC + 1921 CGCAGTATTA CAAAAGGATG TCGCAAACGC TGTTTGCTCC TCTACAAAAC AGACCTTAAA + 1981 ACCCTAAAGG CTTAAGTAGC ACCCTCGCAA GCTCGGGCAA ATCGCTGAAT ATTCCTTTTG + 2041 TCTCCGACCA TCAGGCACCT GAGTCGCTGT CTTTTTCGTG ACATTCAGTT CGCTGCGCTC + 2101 ACGGCTCTGG CAGTGAATGG GGGTAAATGG CACTACAGGC GCGGCGCGCC CAGCTGTCTA + 2161 GGGCGGCGGA TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA TAAAACGAAA + 2221 GGCCCAGTCT TTcgactgag cctttcgttt tatttgatgc ctttaattaa GGCTCGGGAG + 2281 ACCTATCGGT AATAACAGTC CAATCTGGTG TAACTTCGGA ATCGTCCact agtcttggac + 2341 tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg + 2401 gttgccgccg ggcgtttttt attggtgaga atccaggggt ccccaataat tacgatttaa + 2461 attagtagcc cgcctaatga gcgggctttt ttttaattcc cctatttgtt tatttttcta + 2521 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata + 2581 ttgaaaaagg aagagtatga gcattcagca ttttcgtgtg gcgctgattc cgttttttgc + 2641 ggcgttttgc ctgccggtgt ttgcgcatcc ggaaaccctg gtgaaagtga aagatgcgga + 2701 agatcaactg ggtgcgcgcg tgggctatat tgaactggat ctgaacagcg gcaaaattct + 2761 ggaatctttt cgtccggaag aacgttttcc gatgatgagc acctttaaag tgctgctgtg + 2821 cggtgcggtt ctgagccgtg tggatgcggg ccaggaacaa ctgggccgtc gtattcatta + 2881 tagccagaac gatc +// +LOCUS BS_x9x 2819 bp ds-DNA circular 15-OCT-2021 +DEFINITION . +FEATURES Location/Qualifiers + misc_feature 606..662 + /label="LMP" + misc_feature 671..2055 + /label="SEVA_pBR322-ROP" + misc_feature 2070..2174 + /label="SEVA_T1" + misc_feature 2184..2240 + /label="LMS" + misc_feature 2247..2349 + /label="SEVA_T0" + misc_feature join(2376..2819,1..595) + /label="SEVA_Ap" +ORIGIN + 1 gcccggtgac cgaaaaacat ctgaccgatg gcatgaccgt gcgtgaactg tgcagcgcgg + 61 cgattaccat gagcgataac accgcggcga acctgctgct gacgaccatt ggcggtccga + 121 aagaactgac cgcgtttctg cataacatgg gcgatcatgt gacccgtctg gatcgttggg + 181 aaccggaact gaacgaagcg attccgaacg atgaacgtga taccaccatg ccggcagcaa + 241 tggcgaccac cctgcgtaaa ctgctgacgg gtgagctgct gaccctggca agccgccagc + 301 aactgattga ttggatggaa gcggataaag tggcgggtcc gctgctgcgt agcgcgctgc + 361 cggctggctg gtttattgcg gataaaagcg gtgcgggcga acgtggcagc cgtggcatta + 421 ttgcggcgct gggcccggat ggtaaaccga gccgtattgt ggtgatttat accaccggca + 481 gccaggcgac gatggatgaa cgtaaccgtc agattgcgga aattggcgcg agcctgatta + 541 aacattggta aaccgataca attaaaggct ccttttggag cctttttttt tggacgaccc + 601 ttgtcGGCTC GGGTAAGAAC TCGCACTTCG TGGAAACACT ATTATCTGGT GGGTCTCTGT + 661 CCggccggcc ccgtagaaaa gatcaaaggA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA + 721 ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA + 781 GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT + 841 GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA + 901 TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT + 961 ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG + 1021 GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG + 1081 CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA + 1141 AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT + 1201 CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG + 1261 TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC + 1321 TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC + 1381 CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC + 1441 GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG + 1501 TGCGGTATTT CACACCGCAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG + 1561 TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC + 1621 CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC + 1681 AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC + 1741 GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG CAGCGATTCA CAGATGTCTG + 1801 CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA + 1861 TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG + 1921 GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC + 1981 GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG + 2041 TATGGATGCG GCGGGGGCGC GCCCAGCTGT CTAGGGCGGC GGATTTGTCC TACTCAGGAG + 2101 AGCGTTCACC GACAAACAAC AGATAAAACG AAAGGCCCAG TCTTTcgact gagcctttcg + 2161 ttttatttga tgcctttaat taaGGCTCGG GAGACCTATC GGTAATAACA GTCCAATCTG + 2221 GTGTAACTTC GGAATCGTCC actagtcttg gactcctgtt gatagatcca gtaatgacct + 2281 cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg + 2341 agaatccagg ggtccccaat aattacgatt taaattagta gcccgcctaa tgagcgggct + 2401 tttttttaat tcccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat + 2461 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagcattca + 2521 gcattttcgt gtggcgctga ttccgttttt tgcggcgttt tgcctgccgg tgtttgcgca + 2581 tccggaaacc ctggtgaaag tgaaagatgc ggaagatcaa ctgggtgcgc gcgtgggcta + 2641 tattgaactg gatctgaaca gcggcaaaat tctggaatct tttcgtccgg aagaacgttt + 2701 tccgatgatg agcaccttta aagtgctgct gtgcggtgcg gttctgagcc gtgtggatgc + 2761 gggccaggaa caactgggcc gtcgtattca ttatagccag aacgatctgg tggaatata +// +LOCUS BASIC_construct_BS_x7x_ 2963 bp ds-DNA circular 15-OCT-2021 +DEFINITION . +ACCESSION BS_x7x_pKD46 +VERSION BS_x7x_pKD46 +SOURCE synthetic construct + ORGANISM synthetic construct other sequences; artificial sequences. +FEATURES Location/Qualifiers + misc_feature 613..669 + /label="LMP" + CDS 731..1681 + /label="Rep101(pKD46)" + rep_origin 1729..1951 + /label="pSC101 ori" + misc_feature 2221..2325 + /label="SEVA_T1" + misc_feature 2335..2391 + /label="LMS" + misc_feature 2398..2500 + /label="SEVA_T0" + misc_feature join(2527..2963,1..602) + /label="SEVA_Ap" +ORIGIN + 1 gaatatagcc cggtgaccga aaaacatctg accgatggca tgaccgtgcg tgaactgtgc + 61 agcgcggcga ttaccatgag cgataacacc gcggcgaacc tgctgctgac gaccattggc + 121 ggtccgaaag aactgaccgc gtttctgcat aacatgggcg atcatgtgac ccgtctggat + 181 cgttgggaac cggaactgaa cgaagcgatt ccgaacgatg aacgtgatac caccatgccg + 241 gcagcaatgg cgaccaccct gcgtaaactg ctgacgggtg agctgctgac cctggcaagc + 301 cgccagcaac tgattgattg gatggaagcg gataaagtgg cgggtccgct gctgcgtagc + 361 gcgctgccgg ctggctggtt tattgcggat aaaagcggtg cgggcgaacg tggcagccgt + 421 ggcattattg cggcgctggg cccggatggt aaaccgagcc gtattgtggt gatttatacc + 481 accggcagcc aggcgacgat ggatgaacgt aaccgtcaga ttgcggaaat tggcgcgagc + 541 ctgattaaac attggtaaac cgatacaatt aaaggctcct tttggagcct ttttttttgg + 601 acgacccttg tcggctcggg taagaactcg cacttcgtgg aaacactatt atctggtggg + 661 tctctgtcct gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc + 721 cataagaacc tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt + 781 tttgcgtgag ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa + 841 attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc + 901 ttagtccgtt acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca + 961 tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt + 1021 ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt + 1081 aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat + 1141 ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg + 1201 ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt + 1261 atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga + 1321 aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg + 1381 catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag + 1441 ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga + 1501 tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag + 1561 ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct + 1621 ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca + 1681 tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat + 1741 gataattact agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg + 1801 ctggaaaact tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt + 1861 tttttgttta tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa + 1921 aaagaataga tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca + 1981 aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct + 2041 taagtagcac cctcgcaagc tcggttgcgg ccgcaatcgg gcaaatcgct gaatattcct + 2101 tttgtctccg accatcaggc acctgagtcg ctgtcttttt cgtgacattc agttcgctgc + 2161 gctcacggct ctggcagtga atgggggtaa atggcactac aggcgcggcg cgcccagctg + 2221 tctagggcgg cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac + 2281 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctttaa ttaaggctcg + 2341 ggagacctat cggtaataac agtccaatct ggtgtaactt cggaatcgtc cactagtctt + 2401 ggactcctgt tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg + 2461 ctcggttgcc gccgggcgtt ttttattggt gagaatccag gggtccccaa taattacgat + 2521 ttaaattagt agcccgccta atgagcgggc ttttttttaa ttcccctatt tgtttatttt + 2581 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat + 2641 aatattgaaa aaggaagagt atgagcattc agcattttcg tgtggcgctg attccgtttt + 2701 ttgcggcgtt ttgcctgccg gtgtttgcgc atccggaaac cctggtgaaa gtgaaagatg + 2761 cggaagatca actgggtgcg cgcgtgggct atattgaact ggatctgaac agcggcaaaa + 2821 ttctggaatc ttttcgtccg gaagaacgtt ttccgatgat gagcaccttt aaagtgctgc + 2881 tgtgcggtgc ggttctgagc cgtgtggatg cgggccagga acaactgggc cgtcgtattc + 2941 attatagcca gaacgatctg gtg +// \ No newline at end of file