diff --git a/src/NCronJob/Scheduler/QueueWorker.LogMessage.cs b/src/NCronJob/Scheduler/QueueWorker.LogMessage.cs
index ceaaab1..6b57660 100644
--- a/src/NCronJob/Scheduler/QueueWorker.LogMessage.cs
+++ b/src/NCronJob/Scheduler/QueueWorker.LogMessage.cs
@@ -39,7 +39,4 @@ internal sealed partial class QueueWorker
 
     [LoggerMessage(LogLevel.Information, "Job removed from queue: {JobType} at {RunAt}")]
     private partial void LogJobRemovedFromQueue(string jobType, DateTime? runAt);
-
-    [LoggerMessage(LogLevel.Warning, "The UseNCronJob(Async) method was not called. Startup jobs might not have been executed.")]
-    private partial void LogUseNCronJobNotCalled();
 }
diff --git a/src/NCronJob/Scheduler/QueueWorker.cs b/src/NCronJob/Scheduler/QueueWorker.cs
index 265e27a..0ffd092 100644
--- a/src/NCronJob/Scheduler/QueueWorker.cs
+++ b/src/NCronJob/Scheduler/QueueWorker.cs
@@ -122,10 +122,20 @@ protected override async Task ExecuteAsync(CancellationToken stoppingToken)
 
     private void AssertUseNCronJobWasCalled()
     {
-        if (!missingMethodCalledHandler.UseWasCalled)
+        if (missingMethodCalledHandler.UseWasCalled)
         {
-            LogUseNCronJobNotCalled();
+            return;
+        }
+
+        if (jobRegistry.GetAllOneTimeJobs().Count == 0)
+        {
+            return;
         }
+
+        throw new InvalidOperationException(
+            $"""
+            Startup jobs have been registered. However, neither IHost.UseNCronJobAsync(), nor IHost.UseNCronJob() have been been called.
+            """);
     }
 
     private void CreateWorkerQueues(CancellationToken stopToken)
diff --git a/tests/NCronJob.Tests/RunAtStartupJobTests.cs b/tests/NCronJob.Tests/RunAtStartupJobTests.cs
index 6321893..d56c9cb 100644
--- a/tests/NCronJob.Tests/RunAtStartupJobTests.cs
+++ b/tests/NCronJob.Tests/RunAtStartupJobTests.cs
@@ -7,6 +7,26 @@ namespace NCronJob.Tests;
 
 public class RunAtStartupJobTests : JobIntegrationBase
 {
+    [Fact]
+    public async Task UseNCronJobIsMandatoryWhenStartupJobsAreDefined()
+    {
+        var builder = Host.CreateDefaultBuilder();
+        var storage = new Storage();
+        builder.ConfigureServices(services =>
+        {
+            services.AddNCronJob(s => s.AddJob<SimpleJob>().RunAtStartup());
+            services.AddSingleton(_ => storage);
+        });
+
+        using var app = builder.Build();
+
+#pragma warning disable IDISP013 // Await in using
+        Func<Task> act = () => RunApp(app);
+#pragma warning restore IDISP013 // Await in using
+
+        await act.ShouldThrowAsync<InvalidOperationException>();
+    }
+
     [Fact]
     public async Task UseNCronJobShouldTriggerStartupJobs()
     {