diff --git a/packages/main/cypress/specs/Input.cy.ts b/packages/main/cypress/specs/Input.cy.ts
index a909860b5287..50547f81eea1 100644
--- a/packages/main/cypress/specs/Input.cy.ts
+++ b/packages/main/cypress/specs/Input.cy.ts
@@ -1,6 +1,10 @@
 import { html } from "lit";
 import "../../src/Input.js";
 import type Input from "../../src/Input.js";
+import "../../src/features/InputSuggestions.js";
+import "../../src/SuggestionItem.js";
+import "../../src/SuggestionItemCustom.js";
+import "../../src/SuggestionItemGroup.js";
 
 describe("Input Tests", () => {
 	it("tets input event prevention", () => {
@@ -29,4 +33,99 @@ describe("Input Tests", () => {
 			.find("input")
 			.should("have.value", "test");
 	});
+
+	it("tests custom suggestion items tabindex", () => {
+		cy.mount(html`
+			<ui5-input show-suggestions>
+				<ui5-suggestion-item-custom text="Item 1">Item 1</ui5-suggestion-item-custom>
+				<ui5-suggestion-item-custom text="Item 2">Item 2</ui5-suggestion-item-custom>
+				<ui5-suggestion-item-custom text="Item 3">Item 3</ui5-suggestion-item-custom>
+			</ui5-input>
+		`);
+
+		cy.get("[ui5-input]")
+			.as("input");
+
+		cy.get<Input>("@input")
+			.shadow()
+			.find("input")
+			.as("inner");
+
+		cy.get("@inner").realClick();
+		cy.get("@inner").type("i");
+		cy.get("@inner").realPress("ArrowDown");
+
+		cy.get("@input")
+			.find("[ui5-suggestion-item-custom]")
+			.shadow()
+			.find("li")
+			.should("not.have.attr", "tabindex", "0")
+			.should("have.attr", "role", "option");
+	});
+
+	it("tests regular suggestion items tabindex", () => {
+		cy.mount(html`
+			<ui5-input show-suggestions>
+				<ui5-suggestion-item text="Item 1"></ui5-suggestion-item>
+				<ui5-suggestion-item text="Item 2"></ui5-suggestion-item>
+				<ui5-suggestion-item text="Item 3"></ui5-suggestion-item>
+			</ui5-input>
+		`);
+
+		cy.get("[ui5-input]")
+			.as("input");
+
+		cy.get<Input>("@input")
+			.shadow()
+			.find("input")
+			.as("inner");
+
+		cy.get("@inner").realClick();
+		cy.get("@inner").type("i");
+		cy.get("@inner").realPress("ArrowDown");
+
+		cy.get("@input")
+			.find("ui5-suggestion-item")
+			.shadow()
+			.find("li")
+			.should("not.have.attr", "tabindex", "0")
+			.should("have.attr", "role", "option");
+	});
+
+	it("tests suggestion group items tabindex", () => {
+		cy.mount(html`
+			<ui5-input show-suggestions>
+				<ui5-suggestion-item-group header-text="Group 1">
+					<ui5-suggestion-item text="Item 1"></ui5-suggestion-item>
+					<ui5-suggestion-item text="Item 2"></ui5-suggestion-item>
+					<ui5-suggestion-item text="Item 3"></ui5-suggestion-item>
+				</ui5-suggestion-item-group>
+				<ui5-suggestion-item-group header-text="Group 2">
+					<ui5-suggestion-item text="Item 4"></ui5-suggestion-item>
+					<ui5-suggestion-item text="Item 5"></ui5-suggestion-item>
+					<ui5-suggestion-item text="Item 6"></ui5-suggestion-item>
+				</ui5-suggestion-item-group>
+			</ui5-input>
+		`);
+
+		cy.get("[ui5-input]")
+			.as("input");
+
+		cy.get<Input>("@input")
+			.shadow()
+			.find("input")
+			.as("inner");
+
+		cy.get("@inner").realClick();
+		cy.get("@inner").type("i");
+		cy.get("@inner").realPress("ArrowDown");
+
+		cy.get("@input")
+			.find("[ui5-suggestion-item-group]")
+			.shadow()
+			.find("[ui5-li-group-header]")
+			.shadow()
+			.find("ul")
+			.should("not.have.attr", "tabindex", "0");
+	});
 });
diff --git a/packages/main/src/Input.ts b/packages/main/src/Input.ts
index 9554155e65c7..258e7aefba80 100644
--- a/packages/main/src/Input.ts
+++ b/packages/main/src/Input.ts
@@ -737,6 +737,9 @@ class Input extends UI5Element implements SuggestionComponent, IFormInputElement
 
 		if (this.showSuggestions && this.Suggestions?._getPicker()) {
 			this._listWidth = this.Suggestions._getListWidth();
+
+			// disabled ItemNavigation from the list since we are not using it
+			this.Suggestions._getList()._itemNavigation._getItems = () => [];
 		}
 
 		if (this._performTextSelection) {
diff --git a/packages/main/src/ListItemBase.ts b/packages/main/src/ListItemBase.ts
index 3b80487df952..df74c80993ba 100644
--- a/packages/main/src/ListItemBase.ts
+++ b/packages/main/src/ListItemBase.ts
@@ -240,7 +240,7 @@ class ListItemBase extends UI5Element implements ITabbable {
 		return false;
 	}
 
-	get _effectiveTabIndex() {
+	get _effectiveTabIndex(): number | undefined | string {
 		if (!this._focusable) {
 			return -1;
 		}
diff --git a/packages/main/src/SuggestionItem.hbs b/packages/main/src/SuggestionItem.hbs
index c474814b1cce..8838303bb378 100644
--- a/packages/main/src/SuggestionItem.hbs
+++ b/packages/main/src/SuggestionItem.hbs
@@ -11,4 +11,8 @@
 			{{/if}}
 		</div>
 	</div>
+{{/inline}}
+
+{{#*inline "listItemAttributes"}}
+	role="option"
 {{/inline}}
\ No newline at end of file
diff --git a/packages/main/src/SuggestionItem.ts b/packages/main/src/SuggestionItem.ts
index 5c69d934eba3..ceec2eb6326b 100644
--- a/packages/main/src/SuggestionItem.ts
+++ b/packages/main/src/SuggestionItem.ts
@@ -55,6 +55,10 @@ class SuggestionItem extends ListItemBase implements IInputSuggestionItemSelecta
 			this.setAttribute("desktop", "");
 		}
 	}
+
+	get _effectiveTabIndex(): string {
+		return "-1";
+	}
 }
 
 SuggestionItem.define();
diff --git a/packages/main/src/SuggestionItemCustom.hbs b/packages/main/src/SuggestionItemCustom.hbs
index fb649c1cafd5..d84e85dd3be2 100644
--- a/packages/main/src/SuggestionItemCustom.hbs
+++ b/packages/main/src/SuggestionItemCustom.hbs
@@ -3,3 +3,7 @@
 {{#*inline "listItemContent"}}
 	<slot></slot>
 {{/inline}}
+
+{{#*inline "listItemAttributes"}}
+	role="option"
+{{/inline}}
\ No newline at end of file
diff --git a/packages/main/src/SuggestionItemCustom.ts b/packages/main/src/SuggestionItemCustom.ts
index 99d0910d0126..64fc744478da 100644
--- a/packages/main/src/SuggestionItemCustom.ts
+++ b/packages/main/src/SuggestionItemCustom.ts
@@ -48,6 +48,10 @@ class SuggestionItemCustom extends ListItemBase implements IInputSuggestionItemS
 	 */
 	@slot({ type: Node, "default": true, invalidateOnChildChange: true })
 	content!: Array<Node>;
+
+	get _effectiveTabIndex(): string {
+		return "-1";
+	}
 }
 
 SuggestionItemCustom.define();