diff --git a/src/components/multi-action-button/multi-action-button.component.tsx b/src/components/multi-action-button/multi-action-button.component.tsx
index 69e6b6bdff..7873a76280 100644
--- a/src/components/multi-action-button/multi-action-button.component.tsx
+++ b/src/components/multi-action-button/multi-action-button.component.tsx
@@ -58,6 +58,8 @@ export const MultiActionButton = ({
   const handleClick = (
     ev: React.MouseEvent<HTMLButtonElement | HTMLAnchorElement>
   ) => {
+    // ensure button is focused when clicked (Safari)
+    buttonRef.current?.focus();
     showButtons();
     handleInsideClick();
     if (onClick) {
diff --git a/src/components/multi-action-button/multi-action-button.pw.tsx b/src/components/multi-action-button/multi-action-button.pw.tsx
index 1a5fe126a6..a9cc7aba5a 100644
--- a/src/components/multi-action-button/multi-action-button.pw.tsx
+++ b/src/components/multi-action-button/multi-action-button.pw.tsx
@@ -15,7 +15,6 @@ import {
 import {
   assertCssValueIsApproximately,
   checkAccessibility,
-  continuePressingTAB,
 } from "../../../playwright/support/helper";
 import { SIZE, CHARACTERS } from "../../../playwright/support/constants";
 import {
@@ -265,7 +264,6 @@ test.describe("Functional tests", () => {
     const listButton1 = getDataElementByValue(page, "additional-buttons")
       .getByRole("button")
       .nth(0);
-    await continuePressingTAB(page, 2);
     await page.keyboard.press("ArrowUp");
     await expect(listButton1).toBeFocused();
     await page.keyboard.press("ArrowUp");
@@ -290,8 +288,8 @@ test.describe("Functional tests", () => {
     const listButton2 = getDataElementByValue(page, "additional-buttons")
       .getByRole("button")
       .nth(1);
-    await continuePressingTAB(page, 2);
-    await expect(listButton2).toBeFocused();
+
+    await listButton2.focus();
     await page.keyboard.press("Shift+Tab");
     await expect(listButton1).toBeFocused();
     await page.keyboard.press("Shift+Tab");
@@ -313,8 +311,8 @@ test.describe("Functional tests", () => {
     const listButton3 = getDataElementByValue(page, "additional-buttons")
       .getByRole("button")
       .nth(2);
-    await continuePressingTAB(page, 3);
-    await expect(listButton3).toBeFocused();
+
+    await listButton3.focus();
     await page.keyboard.press("ArrowDown");
     await expect(listButton3).toBeFocused();
   });
@@ -325,7 +323,10 @@ test.describe("Functional tests", () => {
   }) => {
     await mount(<MultiActionTwoButtons />);
 
-    await page.keyboard.press("Tab");
+    const actionButton = getComponent(page, "multi-action-button")
+      .first()
+      .locator("button");
+    await actionButton.click();
     const listButton1 = getDataElementByValue(page, "additional-buttons")
       .getByRole("button")
       .nth(0);
@@ -335,7 +336,8 @@ test.describe("Functional tests", () => {
     const listButton3 = getDataElementByValue(page, "additional-buttons")
       .getByRole("button")
       .nth(2);
-    await page.keyboard.press("Space");
+
+    await page.keyboard.press("Tab");
     await expect(listButton1).toBeFocused();
     await page.keyboard.press("Tab");
     await expect(listButton2).toBeFocused();
@@ -363,8 +365,8 @@ test.describe("Functional tests", () => {
     const listButton3 = getDataElementByValue(page, "additional-buttons")
       .getByRole("button")
       .nth(2);
-    await continuePressingTAB(page, 3);
-    await expect(listButton3).toBeFocused();
+
+    await listButton3.focus();
     await page.keyboard.down("Meta");
     await page.keyboard.press("ArrowUp");
     await page.keyboard.up("Meta");
@@ -387,8 +389,8 @@ test.describe("Functional tests", () => {
     const listButton3 = getDataElementByValue(page, "additional-buttons")
       .getByRole("button")
       .nth(2);
-    await continuePressingTAB(page, 3);
-    await expect(listButton3).toBeFocused();
+
+    await listButton3.focus();
     await page.keyboard.down("Control");
     await page.keyboard.press("ArrowUp");
     await page.keyboard.up("Control");
@@ -411,8 +413,8 @@ test.describe("Functional tests", () => {
     const listButton3 = getDataElementByValue(page, "additional-buttons")
       .getByRole("button")
       .nth(2);
-    await continuePressingTAB(page, 3);
-    await expect(listButton3).toBeFocused();
+
+    await listButton3.focus();
     await page.keyboard.press("Home");
     await expect(listButton1).toBeFocused();
   });
@@ -545,7 +547,7 @@ test.describe(
       const listButton1 = getDataElementByValue(page, "additional-buttons")
         .getByRole("button")
         .nth(0);
-      await continuePressingTAB(page, 2);
+
       await page.keyboard.press("ArrowUp");
       await expect(listButton1).toBeFocused();
       await page.keyboard.press("ArrowUp");
@@ -570,8 +572,8 @@ test.describe(
       const listButton2 = getDataElementByValue(page, "additional-buttons")
         .getByRole("button")
         .nth(1);
-      await continuePressingTAB(page, 2);
-      await expect(listButton2).toBeFocused();
+
+      await listButton2.focus();
       await page.keyboard.press("Shift+Tab");
       await expect(listButton1).toBeFocused();
       await page.keyboard.press("Shift+Tab");
@@ -593,8 +595,8 @@ test.describe(
       const listButton3 = getDataElementByValue(page, "additional-buttons")
         .getByRole("button")
         .nth(2);
-      await continuePressingTAB(page, 3);
-      await expect(listButton3).toBeFocused();
+
+      await listButton3.focus();
       await page.keyboard.press("ArrowDown");
       await expect(listButton3).toBeFocused();
     });
@@ -605,7 +607,10 @@ test.describe(
     }) => {
       await mount(<WithWrapperTwoButtons />);
 
-      await page.keyboard.press("Tab");
+      const actionButton = getComponent(page, "multi-action-button")
+        .first()
+        .locator("button");
+      await actionButton.click();
       const listButton1 = getDataElementByValue(page, "additional-buttons")
         .getByRole("button")
         .nth(0);
@@ -615,7 +620,8 @@ test.describe(
       const listButton3 = getDataElementByValue(page, "additional-buttons")
         .getByRole("button")
         .nth(2);
-      await page.keyboard.press("Space");
+
+      await page.keyboard.press("Tab");
       await expect(listButton1).toBeFocused();
       await page.keyboard.press("Tab");
       await expect(listButton2).toBeFocused();
@@ -643,8 +649,8 @@ test.describe(
       const listButton3 = getDataElementByValue(page, "additional-buttons")
         .getByRole("button")
         .nth(2);
-      await continuePressingTAB(page, 3);
-      await expect(listButton3).toBeFocused();
+
+      await listButton3.focus();
       await page.keyboard.down("Meta");
       await page.keyboard.press("ArrowUp");
       await page.keyboard.up("Meta");
@@ -667,8 +673,8 @@ test.describe(
       const listButton3 = getDataElementByValue(page, "additional-buttons")
         .getByRole("button")
         .nth(2);
-      await continuePressingTAB(page, 3);
-      await expect(listButton3).toBeFocused();
+
+      await listButton3.focus();
       await page.keyboard.down("Control");
       await page.keyboard.press("ArrowUp");
       await page.keyboard.up("Control");
@@ -691,8 +697,8 @@ test.describe(
       const listButton3 = getDataElementByValue(page, "additional-buttons")
         .getByRole("button")
         .nth(2);
-      await continuePressingTAB(page, 3);
-      await expect(listButton3).toBeFocused();
+
+      await listButton3.focus();
       await page.keyboard.press("Home");
       await expect(listButton1).toBeFocused();
     });
diff --git a/src/components/split-button/split-button.component.tsx b/src/components/split-button/split-button.component.tsx
index a8fc6a888d..ca8a690134 100644
--- a/src/components/split-button/split-button.component.tsx
+++ b/src/components/split-button/split-button.component.tsx
@@ -102,12 +102,18 @@ export const SplitButton = ({
     ...filterOutStyledSystemSpacingProps(rest),
   };
 
+  const handleToggleClick = () => {
+    // ensure button is focused when clicked (Safari)
+    toggleButton.current?.focus();
+    showButtons();
+  };
+
   const toggleButtonProps = {
     disabled,
     displayed: showAdditionalButtons,
     onTouchStart: showButtons,
     onKeyDown: handleToggleButtonKeyDown,
-    onClick: showButtons,
+    onClick: handleToggleClick,
     buttonType,
     size,
   };
diff --git a/src/hooks/__internal__/useChildButtons/useChildButtons.tsx b/src/hooks/__internal__/useChildButtons/useChildButtons.tsx
index 29c9b50288..9557ae9f8f 100644
--- a/src/hooks/__internal__/useChildButtons/useChildButtons.tsx
+++ b/src/hooks/__internal__/useChildButtons/useChildButtons.tsx
@@ -8,10 +8,13 @@ const useChildButtons = (
 ) => {
   const [showAdditionalButtons, setShowAdditionalButtons] = useState(false);
   const [minWidth, setMinWidth] = useState(0);
+  const [
+    focusFirstChildButtonOnOpen,
+    setFocusFirstChildButtonOnOpen,
+  ] = useState(false);
 
   const buttonNode = useRef<HTMLDivElement>(null);
   const childrenContainer = useRef<HTMLUListElement>(null);
-  const focusFirstChildButtonOnOpen = useRef(false);
 
   const hideButtons = useCallback(() => {
     setShowAdditionalButtons(false);
@@ -38,32 +41,28 @@ const useChildButtons = (
 
   useEffect(() => {
     const firstChild = getButtonChildren()?.[0];
-    if (
-      focusFirstChildButtonOnOpen.current &&
-      showAdditionalButtons &&
-      firstChild
-    ) {
-      focusFirstChildButtonOnOpen.current = false;
+    if (focusFirstChildButtonOnOpen && showAdditionalButtons && firstChild) {
+      setFocusFirstChildButtonOnOpen(false);
       firstChild.focus();
     }
-  }, [showAdditionalButtons, getButtonChildren]);
+  }, [showAdditionalButtons, getButtonChildren, focusFirstChildButtonOnOpen]);
 
   const handleToggleButtonKeyDown = (
     ev: React.KeyboardEvent<HTMLButtonElement>
   ) => {
-    if (
+    const isToggleKey =
       Events.isEnterKey(ev) ||
       Events.isSpaceKey(ev) ||
       Events.isDownKey(ev) ||
-      Events.isUpKey(ev)
-    ) {
+      Events.isUpKey(ev);
+
+    if (isToggleKey || (showAdditionalButtons && Events.isTabKey(ev))) {
       ev.preventDefault();
+      setFocusFirstChildButtonOnOpen(true);
 
-      if (!showAdditionalButtons) {
+      if (isToggleKey && !showAdditionalButtons) {
         showButtons();
       }
-
-      focusFirstChildButtonOnOpen.current = true;
     }
   };