From ca8903133661f13f71ebbde96421dadfb51f0351 Mon Sep 17 00:00:00 2001 From: jenna-tomkinson Date: Wed, 31 Jan 2024 15:12:02 -0700 Subject: [PATCH 1/4] rerun Plate 5 data with QC --- .../analysis_output/Plate_5/Plate_5_nf1_analysis.sqlite | 4 ++-- 3.processing_features/data/bulk_profiles/Plate_5_bulk.parquet | 4 ++-- .../data/bulk_profiles/Plate_5_bulk_annotated.parquet | 4 ++-- .../data/bulk_profiles/Plate_5_bulk_feature_selected.parquet | 4 ++-- .../data/bulk_profiles/Plate_5_bulk_normalized.parquet | 4 ++-- 3.processing_features/data/converted_data/Plate_5.parquet | 4 ++-- .../single_cell_profiles/Plate_5_bulk_camerons_method.parquet | 4 ++-- .../data/single_cell_profiles/Plate_5_sc_annotated.parquet | 4 ++-- .../single_cell_profiles/Plate_5_sc_feature_selected.parquet | 4 ++-- .../data/single_cell_profiles/Plate_5_sc_normalized.parquet | 4 ++-- 10 files changed, 20 insertions(+), 20 deletions(-) diff --git a/2.cellprofiler_analysis/analysis_output/Plate_5/Plate_5_nf1_analysis.sqlite b/2.cellprofiler_analysis/analysis_output/Plate_5/Plate_5_nf1_analysis.sqlite index 199f1be..d3e0b7b 100644 --- a/2.cellprofiler_analysis/analysis_output/Plate_5/Plate_5_nf1_analysis.sqlite +++ b/2.cellprofiler_analysis/analysis_output/Plate_5/Plate_5_nf1_analysis.sqlite @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:6a9e8afa1cf56dcbf28eb04374c0040f730068a05ce37c2fecb35409cb0151ac -size 244957184 +oid sha256:776d5e804fe485f68fd6b013e2c28469de4b99768f2759053c04a8c4f332444a +size 236130304 diff --git a/3.processing_features/data/bulk_profiles/Plate_5_bulk.parquet b/3.processing_features/data/bulk_profiles/Plate_5_bulk.parquet index 0daf71c..503d7d2 100644 --- a/3.processing_features/data/bulk_profiles/Plate_5_bulk.parquet +++ b/3.processing_features/data/bulk_profiles/Plate_5_bulk.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:4228b887ac4ebc66b1bffc421bb841ffc8af77de752bd314228a873e7e9b9e6d -size 3577277 +oid sha256:e3c74cd093d09532b6f6a8b0f74ab714e1889904ad9817b7d40068a0745286ab +size 3577722 diff --git a/3.processing_features/data/bulk_profiles/Plate_5_bulk_annotated.parquet b/3.processing_features/data/bulk_profiles/Plate_5_bulk_annotated.parquet index 16e8ee0..1538d82 100644 --- a/3.processing_features/data/bulk_profiles/Plate_5_bulk_annotated.parquet +++ b/3.processing_features/data/bulk_profiles/Plate_5_bulk_annotated.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:ec9b8dbc22506ad9adc5ceb01b61b289bcc4dcd149d47631eebfcfbe787d7336 -size 3580014 +oid sha256:25b393337bf4ea800ab42cbb96191a7da49230ef5efb51b0b4bf5aec7f94e160 +size 3580436 diff --git a/3.processing_features/data/bulk_profiles/Plate_5_bulk_feature_selected.parquet b/3.processing_features/data/bulk_profiles/Plate_5_bulk_feature_selected.parquet index 19e5b3e..830d402 100644 --- a/3.processing_features/data/bulk_profiles/Plate_5_bulk_feature_selected.parquet +++ b/3.processing_features/data/bulk_profiles/Plate_5_bulk_feature_selected.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:b8ecb8f5422bc1bd82737360c78d8daad23b460e1021cbe0f6b7a10394e750a9 -size 1806100 +oid sha256:988f95e04f630939ef7dd058f2a7f240a6d64d095a209a81399b797ba8b112c9 +size 1814018 diff --git a/3.processing_features/data/bulk_profiles/Plate_5_bulk_normalized.parquet b/3.processing_features/data/bulk_profiles/Plate_5_bulk_normalized.parquet index 3f560e5..a08fc47 100644 --- a/3.processing_features/data/bulk_profiles/Plate_5_bulk_normalized.parquet +++ b/3.processing_features/data/bulk_profiles/Plate_5_bulk_normalized.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:acadbc0daa38bbb2ec14ba368b596ff4735214ac298b1c34b3318e9c6d074930 -size 3603020 +oid sha256:c24e249308dc97ac2371bb4fdf65fe839ac41a0cec89d02574811968c00e7a5b +size 3603591 diff --git a/3.processing_features/data/converted_data/Plate_5.parquet b/3.processing_features/data/converted_data/Plate_5.parquet index 671c672..422d2e2 100644 --- a/3.processing_features/data/converted_data/Plate_5.parquet +++ b/3.processing_features/data/converted_data/Plate_5.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:3ff06ae2f2e832885448b0e8e62a9f110bb5fd24edd249b9dbe22601877b4f55 -size 163230803 +oid sha256:84ab12d4cf2d56d964aa662bb5ec7161dcead4a9f9d86d333cffb945acfd4031 +size 157419890 diff --git a/3.processing_features/data/single_cell_profiles/Plate_5_bulk_camerons_method.parquet b/3.processing_features/data/single_cell_profiles/Plate_5_bulk_camerons_method.parquet index 7b48223..83a5936 100644 --- a/3.processing_features/data/single_cell_profiles/Plate_5_bulk_camerons_method.parquet +++ b/3.processing_features/data/single_cell_profiles/Plate_5_bulk_camerons_method.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:318d79d45d337774bd98f5cdacb4c51f3ff9aa05cf1e97111e489653729a2b26 -size 1846571 +oid sha256:71c0f7377c94450f00bd9376a7f1a952e51e39cb0ab435e589f2fd5b26c1fd1a +size 1847931 diff --git a/3.processing_features/data/single_cell_profiles/Plate_5_sc_annotated.parquet b/3.processing_features/data/single_cell_profiles/Plate_5_sc_annotated.parquet index b469748..1a17b03 100644 --- a/3.processing_features/data/single_cell_profiles/Plate_5_sc_annotated.parquet +++ b/3.processing_features/data/single_cell_profiles/Plate_5_sc_annotated.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:107413be1d5f23fd60b14f2abffaf1413f8250db3daaad24103485171877fca7 -size 163204088 +oid sha256:b0b6e9ed7432269a359316a5a4bb82b47c3581b4ead98fe0c7ae9516e9bc2380 +size 157423593 diff --git a/3.processing_features/data/single_cell_profiles/Plate_5_sc_feature_selected.parquet b/3.processing_features/data/single_cell_profiles/Plate_5_sc_feature_selected.parquet index cc900ad..5c0e603 100644 --- a/3.processing_features/data/single_cell_profiles/Plate_5_sc_feature_selected.parquet +++ b/3.processing_features/data/single_cell_profiles/Plate_5_sc_feature_selected.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:a176d10f02117f9e0fe3354fa685a1d81c912ef833341a5d06eaa912abab8403 -size 88471237 +oid sha256:b99b53c921787bedaee3696caecab5a175e1b466b266b31b6821b0cde6d1a23f +size 85384055 diff --git a/3.processing_features/data/single_cell_profiles/Plate_5_sc_normalized.parquet b/3.processing_features/data/single_cell_profiles/Plate_5_sc_normalized.parquet index de660e1..037d7da 100644 --- a/3.processing_features/data/single_cell_profiles/Plate_5_sc_normalized.parquet +++ b/3.processing_features/data/single_cell_profiles/Plate_5_sc_normalized.parquet @@ -1,3 +1,3 @@ version https://git-lfs.github.com/spec/v1 -oid sha256:8586d250899626b009bba7b5498f4b6d648464bcf7d23d862dadb8b2d19aeb1b -size 164743477 +oid sha256:7caa8a1895d5d772d3f32c2b41ac44cc1976e36a64f6f070df3494ae9927a12a +size 158920061 From f063ee2ddc66dfb603b0bb2c31240dd2e6851e50 Mon Sep 17 00:00:00 2001 From: jenna-tomkinson Date: Thu, 1 Feb 2024 13:28:36 -0700 Subject: [PATCH 2/4] update environment --- nf1_cellpainting_env.yml | 7 ++++--- 1 file changed, 4 insertions(+), 3 deletions(-) diff --git a/nf1_cellpainting_env.yml b/nf1_cellpainting_env.yml index f4ab1ab..472defd 100644 --- a/nf1_cellpainting_env.yml +++ b/nf1_cellpainting_env.yml @@ -13,6 +13,7 @@ dependencies: - conda-forge::numpy - conda-forge::pyyaml - conda-forge::matplotlib +- conda-forge::seaborn - conda-forge::pandas # this package must be hardcoded to work with Mac - conda-forge::mysqlclient=1.4.6 @@ -29,7 +30,7 @@ dependencies: - conda-forge::wxpython=4.1.0 - conda-forge::sentry-sdk=0.18.0 - pip: - # must have 4.2.4 or higher (4.2.4 was used in the analysis at this time) - - cellprofiler>=4.2.4 - - Cytotable + # Install CellProfiler (do not install higher due to possible differences in output) + - cellprofiler==4.2.4 + - CytoTable - pycytominer From 1f78c55d5f9f746715f223fd98b5234327c85af6 Mon Sep 17 00:00:00 2001 From: jenna-tomkinson Date: Thu, 1 Feb 2024 13:29:51 -0700 Subject: [PATCH 3/4] Add QC and rerunning plate 5 --- 1.cellprofiler_ic/README.md | 27 +- .../0.whole_image_qc.ipynb | 175 ++ .../image_quality_control/1.evaluate_qc.ipynb | 1242 ++++++++++++ .../image_quality_control/2.qc_report.ipynb | 679 +++++++ .../misclassified_cells.parquet | 3 + .../Plate_3_channels_blur_density.png | Bin 0 -> 258487 bytes .../Plate_3_prime_channels_blur_density.png | Bin 0 -> 261718 bytes .../Plate_4_channels_blur_density.png | Bin 0 -> 260359 bytes .../Plate_5_channels_blur_density.png | Bin 0 -> 272993 bytes .../Plate_5_flagged_fov_per_well_chart.png | Bin 0 -> 292182 bytes .../Plate_5_per_site_flagged_fov.png | Bin 0 -> 102135 bytes .../Plate_5_platemap_flagged_fov_per_well.png | Bin 0 -> 484519 bytes .../all_channels_combined_density.png | Bin 0 -> 969079 bytes .../qc_figures/blur_outliers_per_plate.png | Bin 0 -> 667134 bytes .../saturation_outliers_per_plate.png | Bin 0 -> 332778 bytes .../qc_results/Plate_3/Experiment.csv | 125 ++ .../qc_results/Plate_3/Image.csv | 1801 +++++++++++++++++ .../qc_results/Plate_3_prime/Experiment.csv | 125 ++ .../qc_results/Plate_3_prime/Image.csv | 1801 +++++++++++++++++ .../qc_results/Plate_4/Experiment.csv | 125 ++ .../qc_results/Plate_4/Image.csv | 1501 ++++++++++++++ .../qc_results/Plate_5/Experiment.csv | 125 ++ .../qc_results/Plate_5/Image.csv | 1513 ++++++++++++++ .../scripts/0.whole_image_qc.py | 82 + .../scripts/1.evaluate_qc.py | 391 ++++ .../scripts/2.qc_report.py | 207 ++ 1.cellprofiler_ic/nf1_ic.ipynb | 32 +- 1.cellprofiler_ic/nf1_ic.sh | 9 +- 1.cellprofiler_ic/nf1_quality_control.sh | 14 + .../{ => pipelines}/NF1_illum_3channel.cppipe | 0 .../{ => pipelines}/NF1_illum_4channel.cppipe | 166 +- .../pipelines/whole_image_qc.cppipe | 115 ++ 1.cellprofiler_ic/scripts/nf1_ic.py | 10 +- .../1.pycytominer_bulk_pipelines.ipynb | 2 +- .../2.pycytominer_singlecell_pipelines.ipynb | 2 +- 35 files changed, 10220 insertions(+), 52 deletions(-) create mode 100644 1.cellprofiler_ic/image_quality_control/0.whole_image_qc.ipynb create mode 100644 1.cellprofiler_ic/image_quality_control/1.evaluate_qc.ipynb create mode 100644 1.cellprofiler_ic/image_quality_control/2.qc_report.ipynb create mode 100644 1.cellprofiler_ic/image_quality_control/misclassified_cells.parquet create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/Plate_3_channels_blur_density.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/Plate_3_prime_channels_blur_density.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/Plate_4_channels_blur_density.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/Plate_5_channels_blur_density.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/Plate_5_flagged_fov_per_well_chart.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/Plate_5_per_site_flagged_fov.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/Plate_5_platemap_flagged_fov_per_well.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/all_channels_combined_density.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/blur_outliers_per_plate.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_figures/saturation_outliers_per_plate.png create mode 100644 1.cellprofiler_ic/image_quality_control/qc_results/Plate_3/Experiment.csv create mode 100644 1.cellprofiler_ic/image_quality_control/qc_results/Plate_3/Image.csv create mode 100644 1.cellprofiler_ic/image_quality_control/qc_results/Plate_3_prime/Experiment.csv create mode 100644 1.cellprofiler_ic/image_quality_control/qc_results/Plate_3_prime/Image.csv create mode 100644 1.cellprofiler_ic/image_quality_control/qc_results/Plate_4/Experiment.csv create mode 100644 1.cellprofiler_ic/image_quality_control/qc_results/Plate_4/Image.csv create mode 100644 1.cellprofiler_ic/image_quality_control/qc_results/Plate_5/Experiment.csv create mode 100644 1.cellprofiler_ic/image_quality_control/qc_results/Plate_5/Image.csv create mode 100644 1.cellprofiler_ic/image_quality_control/scripts/0.whole_image_qc.py create mode 100644 1.cellprofiler_ic/image_quality_control/scripts/1.evaluate_qc.py create mode 100644 1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.py create mode 100644 1.cellprofiler_ic/nf1_quality_control.sh rename 1.cellprofiler_ic/{ => pipelines}/NF1_illum_3channel.cppipe (100%) rename 1.cellprofiler_ic/{ => pipelines}/NF1_illum_4channel.cppipe (63%) create mode 100644 1.cellprofiler_ic/pipelines/whole_image_qc.cppipe diff --git a/1.cellprofiler_ic/README.md b/1.cellprofiler_ic/README.md index a8dc2ab..8f0a4ce 100644 --- a/1.cellprofiler_ic/README.md +++ b/1.cellprofiler_ic/README.md @@ -1,9 +1,28 @@ -# Perform illumination correction and save corrected images +# Perform whole image quality control and illumination correction and save corrected images -In this module, we perform illumination correction (IC) on images for each plate and save the corrected images into new folders. +In this module, we perform whole image quality control (QC) evalutation, illumination correction (IC) on images for each plate and save the corrected images into new folders. Images are saved as 16-bit depth, which is the same as the raw data. -## CellProfiler Pipeline +## Whole image QC + +To remove poor quality images, we use the `MeasureImageQuality` module from CellProfiler to extract blur and saturation metrics and generate optimal thresholds for detection using the z-scoring method. + +Blur metrics will detect both out-of-focus and empty images. +Saturation metrics will detect large artifacts or overly saturated/blown out channels. + +### Run the `image_quality_control` notebooks + +To process, evaluate, and generate a QC report for the NF1 data, you can run the below script to run the notebooks. + +```bash +# Run this script in terminal +# move to the 1.cellprofiler_ic directory to access the `sh` script +cd 1.cellprofiler_ic +# run the notebook as a python script +source nf1_quality_control.sh +``` + +## Illumination Correction Due to there being differences in channel number between plates, we have two different illumination correction pipelines. @@ -13,7 +32,7 @@ Due to there being differences in channel number between plates, we have two dif **Note:** The parameters for correction between pipelines in the same channel might be slightly different. But, the parameters in both perform and output the best corrected images at this point -## Run the `nf1_ic` notebook +### Run the `nf1_ic` notebook To calculate and apply an IC function on each channel, run the [nf1_ic.ipynb](nf1_ic.ipynb) notebook as a python script using the code block below: diff --git a/1.cellprofiler_ic/image_quality_control/0.whole_image_qc.ipynb b/1.cellprofiler_ic/image_quality_control/0.whole_image_qc.ipynb new file mode 100644 index 0000000..13e93de --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/0.whole_image_qc.ipynb @@ -0,0 +1,175 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "# Run whole image QC pipeline in CellProfiler\n" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Import libraries" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "metadata": {}, + "outputs": [], + "source": [ + "import pathlib\n", + "import pprint\n", + "\n", + "import sys\n", + "\n", + "sys.path.append(\"../../utils\")\n", + "import cp_parallel" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Set paths and variables" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "['Plate_4', 'Plate_1', 'Plate_3', 'Plate_2', 'Plate_5', 'Plate_3_prime']\n", + "There are a total of 6 plates. The names of the plates are:\n", + "Plate_4\n", + "Plate_1\n", + "Plate_3\n", + "Plate_2\n", + "Plate_5\n", + "Plate_3_prime\n" + ] + } + ], + "source": [ + "# set the run type for the parallelization\n", + "run_name = \"quality_control\"\n", + "\n", + "# set path for pipeline for illumination correction\n", + "path_to_pipeline = pathlib.Path(\"../pipelines/whole_image_qc.cppipe\").resolve(strict=True)\n", + "\n", + "# set main output dir for all plates if it doesn't exist\n", + "output_dir = pathlib.Path(\"./qc_results\")\n", + "output_dir.mkdir(exist_ok=True)\n", + "\n", + "# directory where images are located within folders\n", + "images_dir = pathlib.Path(\"../../0.download_data\")\n", + "\n", + "# list for plate names based on folders to use to create dictionary\n", + "plate_names = []\n", + "# iterate through 0.download_data and append plate names from folder names that contain image data from that plate\n", + "for file_path in images_dir.iterdir():\n", + " if str(file_path.stem).startswith(\"Plate\"):\n", + " plate_names.append(str(file_path.stem))\n", + "\n", + "print(plate_names)\n", + "print(\"There are a total of\", len(plate_names), \"plates. The names of the plates are:\")\n", + "for plate in plate_names:\n", + " print(plate)" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Generate dictionary with plate info to run CellProfiler" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "{ 'Plate_3': { 'path_to_images': PosixPath('/home/jenna/nf1_cellpainting_data/0.download_data/Plate_3'),\n", + " 'path_to_output': PosixPath('qc_results/Plate_3'),\n", + " 'path_to_pipeline': PosixPath('/home/jenna/nf1_cellpainting_data/1.cellprofiler_ic/pipelines/whole_image_qc.cppipe')},\n", + " 'Plate_3_prime': { 'path_to_images': PosixPath('/home/jenna/nf1_cellpainting_data/0.download_data/Plate_3_prime'),\n", + " 'path_to_output': PosixPath('qc_results/Plate_3_prime'),\n", + " 'path_to_pipeline': PosixPath('/home/jenna/nf1_cellpainting_data/1.cellprofiler_ic/pipelines/whole_image_qc.cppipe')},\n", + " 'Plate_4': { 'path_to_images': PosixPath('/home/jenna/nf1_cellpainting_data/0.download_data/Plate_4'),\n", + " 'path_to_output': PosixPath('qc_results/Plate_4'),\n", + " 'path_to_pipeline': PosixPath('/home/jenna/nf1_cellpainting_data/1.cellprofiler_ic/pipelines/whole_image_qc.cppipe')},\n", + " 'Plate_5': { 'path_to_images': PosixPath('/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5'),\n", + " 'path_to_output': PosixPath('qc_results/Plate_5'),\n", + " 'path_to_pipeline': PosixPath('/home/jenna/nf1_cellpainting_data/1.cellprofiler_ic/pipelines/whole_image_qc.cppipe')}}\n" + ] + } + ], + "source": [ + "# create plate info dictionary with all parts of the CellProfiler CLI command to run in parallel\n", + "plate_info_dictionary = {\n", + " name: {\n", + " \"path_to_images\": pathlib.Path(list(images_dir.rglob(name))[0]).resolve(\n", + " strict=True\n", + " ),\n", + " \"path_to_output\": pathlib.Path(f\"{output_dir}/{name}\"),\n", + " \"path_to_pipeline\": path_to_pipeline,\n", + "\n", + " }\n", + " for name in plate_names if not name=='Plate_1' and not name=='Plate_2' # Do not include pilot datasets\n", + "}\n", + "\n", + "# view the dictionary to assess that all info is added correctly\n", + "pprint.pprint(plate_info_dictionary, indent=4)" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Run QC pipeline in CellProfiler" + ] + }, + { + "cell_type": "code", + "execution_count": null, + "metadata": {}, + "outputs": [], + "source": [ + "cp_parallel.run_cellprofiler_parallel(\n", + " plate_info_dictionary=plate_info_dictionary, run_name=run_name\n", + ")" + ] + } + ], + "metadata": { + "kernelspec": { + "display_name": "cfret_data", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.8.18" + } + }, + "nbformat": 4, + "nbformat_minor": 2 +} diff --git a/1.cellprofiler_ic/image_quality_control/1.evaluate_qc.ipynb b/1.cellprofiler_ic/image_quality_control/1.evaluate_qc.ipynb new file mode 100644 index 0000000..ebe6e9e --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/1.evaluate_qc.ipynb @@ -0,0 +1,1242 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "# Whole image quality control metric evaluation\n", + "\n", + "In this notebook, we will use the outputted QC metrics to start working on developing thresholds using z-score to flag and skip images during CellProfiler illumination correction. We are evaluating plates 3-5 to find generalizable metrics and thresholds for this dataset.\n", + "\n", + "**Blur metric to detect out of focus images** -> PowerLogLogSlope\n", + "\n", + "**Saturation metric to detect large smudges** -> PercentMaximal" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Import libraries" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "metadata": {}, + "outputs": [], + "source": [ + "import pathlib\n", + "import pandas as pd\n", + "import numpy as np\n", + "\n", + "from scipy.stats import zscore\n", + "import matplotlib.pyplot as plt\n", + "import seaborn as sns" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Set paths and load in data frame" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "dict_keys(['Plate_3', 'Plate_3_prime', 'Plate_4', 'Plate_5'])\n", + "(1512, 87)\n" + ] + }, + { + "data": { + "text/html": [ + "
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598300E411-3.6091060.192319DAPIPlate_5
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" + ], + "text/plain": [ + " Metadata_Frame Metadata_Series Metadata_Well Metadata_Site \\\n", + "9106 0 0 D1 4 \n", + "5778 0 0 D6 16 \n", + "42 0 0 B11 2 \n", + "5273 0 0 B6 15 \n", + "5983 0 0 E4 11 \n", + "\n", + " ImageQuality_PowerLogLogSlope ImageQuality_PercentMaximal Channel \\\n", + "9106 -3.932344 0.000090 GFP \n", + "5778 -3.817712 0.523093 DAPI \n", + "42 -3.744752 0.760690 DAPI \n", + "5273 -3.668699 0.744151 DAPI \n", + "5983 -3.609106 0.192319 DAPI \n", + "\n", + " Metadata_Plate \n", + "9106 Plate_3_prime \n", + "5778 Plate_5 \n", + "42 Plate_3 \n", + "5273 Plate_5 \n", + "5983 Plate_5 " + ] + }, + "execution_count": 5, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "# Calculate Z-scores for the column with all plates\n", + "z_scores = zscore(df[\"ImageQuality_PowerLogLogSlope\"])\n", + "\n", + "# Set a threshold for Z-scores (adjust as needed for number of standard deviations away from the mean)\n", + "threshold_z = 2\n", + "\n", + "# Identify outlier rows based on Z-scores above and below the mean since we are using absolute values of the z-scores\n", + "blur_outliers = df[abs(z_scores) > threshold_z]\n", + "\n", + "# Identify unique well + site combos among outliers\n", + "unique_combos = blur_outliers[[\"Metadata_Well\", \"Metadata_Site\"]].drop_duplicates()\n", + "\n", + "print(blur_outliers[\"Channel\"].value_counts())\n", + "# Print example outliers to visualize\n", + "blur_outliers.sort_values(by=\"ImageQuality_PowerLogLogSlope\").head()" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "### Identify threshold values to use to find outliers above and below the mean\n", + "\n", + "**Note:** These values will be used in CellProfiler to flag and not process images. We will round the values to the second decimal to use in the pipeline." + ] + }, + { + "cell_type": "code", + "execution_count": 6, + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Threshold for outliers above the mean: -1.36839084751248\n", + "Threshold for outliers below the mean: -2.4936740062477365\n" + ] + } + ], + "source": [ + "# Calculate the mean and standard deviation\n", + "mean_value = df[\"ImageQuality_PowerLogLogSlope\"].mean()\n", + "std_dev = df[\"ImageQuality_PowerLogLogSlope\"].std()\n", + "\n", + "# Set the threshold multiplier for above and below the mean\n", + "threshold = 2\n", + "\n", + "# Calculate the threshold values\n", + "threshold_value_above_mean = mean_value + threshold * std_dev\n", + "threshold_value_below_mean = mean_value - threshold * std_dev\n", + "\n", + "# Print the calculated threshold values\n", + "print(\"Threshold for outliers above the mean:\", threshold_value_above_mean)\n", + "print(\"Threshold for outliers below the mean:\", threshold_value_below_mean)" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "**We will be using these thresholds in the CellProfiler pipeline. We will set anything `greater` than -1.37 as outliers and anything `below` -2.50 as outliers.**" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "### Generate individual density plots per plate and save" + ] + }, + { + "cell_type": "code", + "execution_count": 7, + "metadata": {}, + "outputs": [], + "source": [ + "# Set the style\n", + "sns.set_style(\"whitegrid\")\n", + "\n", + "# Iterate through each plate\n", + "for plate in df[\"Metadata_Plate\"].unique():\n", + " plate_df = df[df[\"Metadata_Plate\"] == plate]\n", + "\n", + " sns.kdeplot(\n", + " data=plate_df,\n", + " x=\"ImageQuality_PowerLogLogSlope\",\n", + " hue=\"Channel\",\n", + " palette=[\"b\", \"g\", \"r\", \"magenta\"],\n", + " fill=True,\n", + " common_norm=False,\n", + " )\n", + "\n", + " plt.title(f\"Density plots per channel for {plate}\")\n", + " plt.xlabel(\"ImageQuality_PowerLogLogSlope\")\n", + " plt.ylabel(\"Density\")\n", + "\n", + " plt.tight_layout()\n", + " plt.savefig(\n", + " pathlib.Path(f\"{figure_dir}/{plate}_channels_blur_density.png\"), dpi=500\n", + " )\n", + " plt.close() # Close the plot to prevent it from being displayed" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "### Generate density plot with all plates together and save" + ] + }, + { + "cell_type": "code", + "execution_count": 8, + "metadata": {}, + "outputs": [], + "source": [ + "# Number of plates\n", + "num_plates = len(df[\"Metadata_Plate\"].unique())\n", + "\n", + "# Calculate the number of rows and columns for the subplots\n", + "num_rows = 2 # You want 2 rows for the quad order\n", + "num_cols = (num_plates + 1) // num_rows # +1 to round up\n", + "\n", + "# Set the style\n", + "sns.set_style(\"whitegrid\")\n", + "\n", + "# Create subplots with custom layout\n", + "fig, axes = plt.subplots(nrows=num_rows, ncols=num_cols, figsize=(15, 5 * num_rows))\n", + "\n", + "# Flatten the axes array for easier indexing\n", + "axes = axes.flatten()\n", + "\n", + "# Iterate through each plate\n", + "for idx, plate in enumerate(df[\"Metadata_Plate\"].unique()):\n", + " # Plot all channels in the same subplot\n", + " ax = axes[idx]\n", + " plate_df = df[df[\"Metadata_Plate\"] == plate]\n", + " sns.kdeplot(\n", + " data=plate_df,\n", + " x=\"ImageQuality_PowerLogLogSlope\",\n", + " hue=\"Channel\",\n", + " palette=[\"b\", \"g\", \"r\", \"magenta\"],\n", + " fill=True,\n", + " common_norm=False,\n", + " ax=ax,\n", + " )\n", + "\n", + " # Set vertical lines at -2.5 and -1.25\n", + " ax.axvline(x=threshold_value_above_mean, color=\"red\", linestyle=\"--\")\n", + " ax.axvline(x=threshold_value_below_mean, color=\"red\", linestyle=\"--\")\n", + "\n", + " ax.set_title(f\"Density plots for {plate}\")\n", + " ax.set_xlabel(\"ImageQuality_PowerLogLogSlope\")\n", + " ax.set_ylabel(\"Density\")\n", + "\n", + " # Set the x-axis range for the current subplot\n", + " ax.set_xlim(-4.0, 0)\n", + "\n", + "# Remove empty subplots\n", + "for i in range(num_plates, len(axes)):\n", + " fig.delaxes(axes[i])\n", + "\n", + "# Adjust layout\n", + "plt.tight_layout()\n", + "\n", + "# Save the plot\n", + "plt.savefig(figure_dir / \"all_channels_combined_density.png\", dpi=500)\n", + "\n", + "plt.close() # Close the plot to prevent it from being displayed" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "### Visualize the distribution of the identified outliers" + ] + }, + { + "cell_type": "code", + "execution_count": 9, + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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", + "text/plain": [ + "
" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "# Plot outliers as a distribution plot with hue as 'Metadata_Plate'\n", + "sns.set_style(\"whitegrid\")\n", + "plt.figure(figsize=(12, 8))\n", + "\n", + "# Using kdeplot (you can also use distplot)\n", + "sns.kdeplot(\n", + " data=blur_outliers,\n", + " x=\"ImageQuality_PowerLogLogSlope\",\n", + " hue=\"Metadata_Plate\",\n", + " palette=\"viridis\", \n", + " fill=True,\n", + " common_norm=False,\n", + ")\n", + "\n", + "# Set vertical lines at -2.5 and 1.25\n", + "plt.axvline(x=threshold_value_above_mean, color=\"red\", linestyle=\"--\")\n", + "plt.axvline(x=threshold_value_below_mean, color=\"red\", linestyle=\"--\")\n", + "\n", + "plt.title(\"Distribution of Outliers per Plate\")\n", + "plt.xlabel(\"ImageQuality_PowerLogLogSlope\")\n", + "\n", + "plt.tight_layout()\n", + "\n", + "plt.savefig(figure_dir / \"blur_outliers_per_plate.png\", dpi=500)\n", + "\n", + "plt.show()" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Saturation metric\n", + "\n", + "For saturation metrics, we are looking for:\n", + "\n", + "- Smudged images or images containing large artifacts (usually seen in `DNA` channel regardless of stain)\n", + "- Overly saturated channels which can occur in any channel, but specifically we are seeing `Mito` having the most problems with this.\n", + "\n", + "This means that we will only be setting threshold for outliers for all channels just like for blur." + ] + }, + { + "cell_type": "code", + "execution_count": 10, + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "count 26448.000000\n", + "mean 0.164823\n", + "std 0.618692\n", + "min 0.000090\n", + "25% 0.003434\n", + "50% 0.026118\n", + "75% 0.117126\n", + "max 44.787238\n", + "Name: ImageQuality_PercentMaximal, dtype: float64\n" + ] + } + ], + "source": [ + "summary_statistics = df[\"ImageQuality_PercentMaximal\"].describe()\n", + "print(summary_statistics)" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "### Visualize density plot of saturation metric" + ] + }, + { + "cell_type": "code", + "execution_count": 11, + "metadata": {}, + "outputs": [ + { + "data": { + "image/png": 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" + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "# Create a KDE plot with separate lines for each Metadata_Plate\n", + "plt.figure(figsize=(10, 6))\n", + "sns.kdeplot(\n", + " x=np.log1p(df[\"ImageQuality_PercentMaximal\"]), hue=df[\"Metadata_Plate\"], fill=True\n", + ")\n", + "\n", + "# Add a vertical line at the log-transformed value of 1.4\n", + "plt.axvline(x=np.log1p(1.4), color='red', linestyle='--', label='Log-transformed 1.4')\n", + "\n", + "# Set labels and title\n", + "plt.ylabel(\"Density\")\n", + "plt.xlabel(\"Log-transformed Percent Maximal\")\n", + "plt.title(\"KDE Plot of Log-transformed Percent Maximal by Plate\")\n", + "\n", + "plt.savefig(figure_dir / \"saturation_outliers_per_plate.png\", dpi=500)\n", + "\n", + "# Show the plot\n", + "plt.show()" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "### Use z-score to find outliers 2 standard deviations above the mean to view as a data frame" + ] + }, + { + "cell_type": "code", + "execution_count": 12, + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Channel\n", + "CY5 521\n", + "DAPI 54\n", + "RFP 5\n", + "GFP 1\n", + "Name: count, dtype: int64\n" + ] + }, + { + "data": { + "text/html": [ + "
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Metadata_FrameMetadata_SeriesMetadata_WellMetadata_SiteImageQuality_PowerLogLogSlopeImageQuality_PercentMaximalChannelMetadata_Plate
2181000B49-1.6737571.402716CY5Plate_3_prime
2086700E316-2.0721871.409043CY5Plate_3
2443400E98-2.1186621.413290CY5Plate_4
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2222000C919-2.3026021.414284CY5Plate_3_prime
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" + ], + "text/plain": [ + " Metadata_Frame Metadata_Series Metadata_Well Metadata_Site \\\n", + "21810 0 0 B4 9 \n", + "20867 0 0 E3 16 \n", + "24434 0 0 E9 8 \n", + "22574 0 0 E11 22 \n", + "22220 0 0 C9 19 \n", + "\n", + " ImageQuality_PowerLogLogSlope ImageQuality_PercentMaximal Channel \\\n", + "21810 -1.673757 1.402716 CY5 \n", + "20867 -2.072187 1.409043 CY5 \n", + "24434 -2.118662 1.413290 CY5 \n", + "22574 -1.846556 1.414284 CY5 \n", + "22220 -2.302602 1.414284 CY5 \n", + "\n", + " Metadata_Plate \n", + "21810 Plate_3_prime \n", + "20867 Plate_3 \n", + "24434 Plate_4 \n", + "22574 Plate_3_prime \n", + "22220 Plate_3_prime " + ] + }, + "execution_count": 12, + "metadata": {}, + "output_type": "execute_result" + } + ], + "source": [ + "# Calculate Z-scores for the column\n", + "z_scores = zscore(df[\"ImageQuality_PercentMaximal\"])\n", + "\n", + "# Set a threshold for Z-scores (adjust as needed for number of standard deviations away from the mean)\n", + "threshold_z = 2\n", + "\n", + "# Identify outlier rows based on Z-scores greater than as to identify whole images with abnormally high saturated pixels\n", + "saturation_outliers = df[abs(z_scores) > threshold_z]\n", + "\n", + "# Identify unique well + site combos among outliers\n", + "unique_combos = saturation_outliers[\n", + " [\"Metadata_Well\", \"Metadata_Site\"]\n", + "].drop_duplicates()\n", + "\n", + "# Visualize example images through FIJI using data frame identifying outliers\n", + "print(saturation_outliers[\"Channel\"].value_counts())\n", + "saturation_outliers.sort_values(by=\"ImageQuality_PercentMaximal\", ascending=True).head()" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "### Identify threshold values to use to find outliers above the mean\n", + "\n", + "**Note:** This value will be used in CellProfiler to flag and not process images. We will round to the second decimal to use in the pipeline." + ] + }, + { + "cell_type": "code", + "execution_count": 13, + "metadata": {}, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "Threshold for outliers above the mean: 1.4022065573415774\n" + ] + } + ], + "source": [ + "# Calculate the mean and standard deviation\n", + "mean_value = df[\"ImageQuality_PercentMaximal\"].mean()\n", + "std_dev = df[\"ImageQuality_PercentMaximal\"].std()\n", + "\n", + "# Set the threshold multiplier for above the mean\n", + "threshold = 2\n", + "\n", + "# Calculate the threshold values\n", + "threshold_value_above_mean = mean_value + threshold * std_dev\n", + "\n", + "# Print the calculated threshold values\n", + "print(\"Threshold for outliers above the mean:\", threshold_value_above_mean)" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "**We will be using 1.40 as the threshold for anything that `above` this is considered a outlier for all channels.**" + ] + } + ], + "metadata": { + "kernelspec": { + "display_name": "cfret_data_env", + "language": "python", + "name": "python3" + }, + "language_info": { + "codemirror_mode": { + "name": "ipython", + "version": 3 + }, + "file_extension": ".py", + "mimetype": "text/x-python", + "name": "python", + "nbconvert_exporter": "python", + "pygments_lexer": "ipython3", + "version": "3.8.18" + } + }, + "nbformat": 4, + "nbformat_minor": 2 +} diff --git a/1.cellprofiler_ic/image_quality_control/2.qc_report.ipynb b/1.cellprofiler_ic/image_quality_control/2.qc_report.ipynb new file mode 100644 index 0000000..88043a4 --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/2.qc_report.ipynb @@ -0,0 +1,679 @@ +{ + "cells": [ + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "# Whole Image Quality Control Report" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Import libraries" + ] + }, + { + "cell_type": "code", + "execution_count": 1, + "metadata": { + "vscode": { + "languageId": "r" + } + }, + "outputs": [ + { + "name": "stderr", + "output_type": "stream", + "text": [ + "Warning message:\n", + "“package ‘ggplot2’ was built under R version 4.2.3”\n", + "Warning message:\n", + "“package ‘platetools’ was built under R version 4.2.3”\n", + "Warning message:\n", + "“package ‘stringr’ was built under R version 4.2.3”\n", + "Warning message:\n", + "“package ‘arrow’ was built under R version 4.2.3”\n", + "Warning message:\n", + "“package ‘RColorBrewer’ was built under R version 4.2.3”\n" + ] + } + ], + "source": [ + "suppressPackageStartupMessages(library(dplyr))\n", + "suppressPackageStartupMessages(library(ggplot2))\n", + "suppressPackageStartupMessages(library(platetools))\n", + "suppressPackageStartupMessages(library(stringr))\n", + "suppressPackageStartupMessages(library(arrow))\n", + "suppressPackageStartupMessages(library(RColorBrewer))" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Set paths and variables" + ] + }, + { + "cell_type": "code", + "execution_count": 2, + "metadata": { + "vscode": { + "languageId": "r" + } + }, + "outputs": [], + "source": [ + "# Paths to CSV files to generate QC report\n", + "path_to_qc_results <- file.path(\"../Corrected_Images/Corrected_Plate_5/IC_QC_RunImage.csv\")\n", + "\n", + "# Output path for bar chart\n", + "barchart_output_file <- file.path(paste0(\"./qc_figures/Plate_5_flagged_fov_per_well_chart.png\"))\n", + "\n", + "# Output path for platemap\n", + "platemap_output_file <- file.path(paste0(\"./qc_figures/Plate_5_platemap_flagged_fov_per_well.png\"))\n", + "\n", + "# Output path for site bar plot\n", + "site_FOV_output_file <- file.path(paste0(\"./qc_figures/Plate_5_per_site_flagged_fov.png\"))" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Load in outlier and quality control results" + ] + }, + { + "cell_type": "code", + "execution_count": 3, + "metadata": { + "vscode": { + "languageId": "r" + } + }, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "
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A data.frame: 6 × 110
Channel_OrigDAPIChannel_OrigGFPChannel_OrigMitoChannel_OrigRFPExecutionTime_01ImagesExecutionTime_02MetadataExecutionTime_03NamesAndTypesExecutionTime_04GroupsExecutionTime_05MeasureImageQualityExecutionTime_06FlagImage⋯Series_OrigMitoSeries_OrigRFPURL_OrigDAPIURL_OrigGFPURL_OrigMitoURL_OrigRFPWidth_OrigDAPIWidth_OrigGFPWidth_OrigMitoWidth_OrigRFP
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\n" + ], + "text/latex": [ + "A data.frame: 6 × 110\n", + "\\begin{tabular}{r|lllllllllllllllllllll}\n", + " & Channel\\_OrigDAPI & Channel\\_OrigGFP & Channel\\_OrigMito & Channel\\_OrigRFP & ExecutionTime\\_01Images & ExecutionTime\\_02Metadata & ExecutionTime\\_03NamesAndTypes & ExecutionTime\\_04Groups & ExecutionTime\\_05MeasureImageQuality & ExecutionTime\\_06FlagImage & ⋯ & Series\\_OrigMito & Series\\_OrigRFP & URL\\_OrigDAPI & URL\\_OrigGFP & URL\\_OrigMito & URL\\_OrigRFP & Width\\_OrigDAPI & Width\\_OrigGFP & Width\\_OrigMito & Width\\_OrigRFP\\\\\n", + " & & & & & & & & & & & ⋯ & & & & & & & & & & \\\\\n", + "\\hline\n", + "\t1 & -1 & -1 & -1 & -1 & 0 & 0 & 1.14 & 0 & 1.07 & 0 & ⋯ & 0 & 0 & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_1\\_10\\_DAPI\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_2\\_10\\_GFP\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_3\\_10\\_CY5\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_4\\_10\\_RFP\\_001.tif & 1224 & 1224 & 1224 & 1224\\\\\n", + "\t2 & -1 & -1 & -1 & -1 & 0 & 0 & 1.35 & 0 & 1.13 & 0 & ⋯ & 0 & 0 & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_1\\_11\\_DAPI\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_2\\_11\\_GFP\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_3\\_11\\_CY5\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_4\\_11\\_RFP\\_001.tif & 1224 & 1224 & 1224 & 1224\\\\\n", + "\t3 & -1 & -1 & -1 & -1 & 0 & 0 & 0.67 & 0 & 1.21 & 0 & ⋯ & 0 & 0 & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_1\\_12\\_DAPI\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_2\\_12\\_GFP\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_3\\_12\\_CY5\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_4\\_12\\_RFP\\_001.tif & 1224 & 1224 & 1224 & 1224\\\\\n", + "\t4 & -1 & -1 & -1 & -1 & 0 & 0 & 0.60 & 0 & 1.13 & 0 & ⋯ & 0 & 0 & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_1\\_13\\_DAPI\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_2\\_13\\_GFP\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_3\\_13\\_CY5\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_4\\_13\\_RFP\\_001.tif & 1224 & 1224 & 1224 & 1224\\\\\n", + "\t5 & -1 & -1 & -1 & -1 & 0 & 0 & 0.45 & 0 & 1.10 & 0 & ⋯ & 0 & 0 & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_1\\_14\\_DAPI\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_2\\_14\\_GFP\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_3\\_14\\_CY5\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_4\\_14\\_RFP\\_001.tif & 1224 & 1224 & 1224 & 1224\\\\\n", + "\t6 & -1 & -1 & -1 & -1 & 0 & 0 & 0.54 & 0 & 1.03 & 0 & ⋯ & 0 & 0 & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_1\\_15\\_DAPI\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_2\\_15\\_GFP\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_3\\_15\\_CY5\\_001.tif & file:/home/jenna/nf1\\_cellpainting\\_data/0.download\\_data/Plate\\_5/B10\\_01\\_4\\_15\\_RFP\\_001.tif & 1224 & 1224 & 1224 & 1224\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A data.frame: 6 × 110\n", + "\n", + "| | Channel_OrigDAPI <int> | Channel_OrigGFP <int> | Channel_OrigMito <int> | Channel_OrigRFP <int> | ExecutionTime_01Images <dbl> | ExecutionTime_02Metadata <int> | ExecutionTime_03NamesAndTypes <dbl> | ExecutionTime_04Groups <int> | ExecutionTime_05MeasureImageQuality <dbl> | ExecutionTime_06FlagImage <dbl> | ⋯ ⋯ | Series_OrigMito <int> | Series_OrigRFP <int> | URL_OrigDAPI <chr> | URL_OrigGFP <chr> | URL_OrigMito <chr> | URL_OrigRFP <chr> | Width_OrigDAPI <int> | Width_OrigGFP <int> | Width_OrigMito <int> | Width_OrigRFP <int> |\n", + "|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|\n", + "| 1 | -1 | -1 | -1 | -1 | 0 | 0 | 1.14 | 0 | 1.07 | 0 | ⋯ | 0 | 0 | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_10_DAPI_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_10_GFP_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_10_CY5_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_10_RFP_001.tif | 1224 | 1224 | 1224 | 1224 |\n", + "| 2 | -1 | -1 | -1 | -1 | 0 | 0 | 1.35 | 0 | 1.13 | 0 | ⋯ | 0 | 0 | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_11_DAPI_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_11_GFP_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_11_CY5_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_11_RFP_001.tif | 1224 | 1224 | 1224 | 1224 |\n", + "| 3 | -1 | -1 | -1 | -1 | 0 | 0 | 0.67 | 0 | 1.21 | 0 | ⋯ | 0 | 0 | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_12_DAPI_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_12_GFP_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_12_CY5_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_12_RFP_001.tif | 1224 | 1224 | 1224 | 1224 |\n", + "| 4 | -1 | -1 | -1 | -1 | 0 | 0 | 0.60 | 0 | 1.13 | 0 | ⋯ | 0 | 0 | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_13_DAPI_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_13_GFP_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_13_CY5_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_13_RFP_001.tif | 1224 | 1224 | 1224 | 1224 |\n", + "| 5 | -1 | -1 | -1 | -1 | 0 | 0 | 0.45 | 0 | 1.10 | 0 | ⋯ | 0 | 0 | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_14_DAPI_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_14_GFP_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_14_CY5_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_14_RFP_001.tif | 1224 | 1224 | 1224 | 1224 |\n", + "| 6 | -1 | -1 | -1 | -1 | 0 | 0 | 0.54 | 0 | 1.03 | 0 | ⋯ | 0 | 0 | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_15_DAPI_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_15_GFP_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_15_CY5_001.tif | file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_15_RFP_001.tif | 1224 | 1224 | 1224 | 1224 |\n", + "\n" + ], + "text/plain": [ + " Channel_OrigDAPI Channel_OrigGFP Channel_OrigMito Channel_OrigRFP\n", + "1 -1 -1 -1 -1 \n", + "2 -1 -1 -1 -1 \n", + "3 -1 -1 -1 -1 \n", + "4 -1 -1 -1 -1 \n", + "5 -1 -1 -1 -1 \n", + "6 -1 -1 -1 -1 \n", + " ExecutionTime_01Images ExecutionTime_02Metadata ExecutionTime_03NamesAndTypes\n", + "1 0 0 1.14 \n", + "2 0 0 1.35 \n", + "3 0 0 0.67 \n", + "4 0 0 0.60 \n", + "5 0 0 0.45 \n", + "6 0 0 0.54 \n", + " ExecutionTime_04Groups ExecutionTime_05MeasureImageQuality\n", + "1 0 1.07 \n", + "2 0 1.13 \n", + "3 0 1.21 \n", + "4 0 1.13 \n", + "5 0 1.10 \n", + "6 0 1.03 \n", + " ExecutionTime_06FlagImage ⋯ Series_OrigMito Series_OrigRFP\n", + "1 0 ⋯ 0 0 \n", + "2 0 ⋯ 0 0 \n", + "3 0 ⋯ 0 0 \n", + "4 0 ⋯ 0 0 \n", + "5 0 ⋯ 0 0 \n", + "6 0 ⋯ 0 0 \n", + " URL_OrigDAPI \n", + "1 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_10_DAPI_001.tif\n", + "2 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_11_DAPI_001.tif\n", + "3 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_12_DAPI_001.tif\n", + "4 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_13_DAPI_001.tif\n", + "5 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_14_DAPI_001.tif\n", + "6 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_1_15_DAPI_001.tif\n", + " URL_OrigGFP \n", + "1 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_10_GFP_001.tif\n", + "2 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_11_GFP_001.tif\n", + "3 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_12_GFP_001.tif\n", + "4 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_13_GFP_001.tif\n", + "5 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_14_GFP_001.tif\n", + "6 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_2_15_GFP_001.tif\n", + " URL_OrigMito \n", + "1 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_10_CY5_001.tif\n", + "2 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_11_CY5_001.tif\n", + "3 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_12_CY5_001.tif\n", + "4 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_13_CY5_001.tif\n", + "5 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_14_CY5_001.tif\n", + "6 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_3_15_CY5_001.tif\n", + " URL_OrigRFP \n", + "1 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_10_RFP_001.tif\n", + "2 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_11_RFP_001.tif\n", + "3 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_12_RFP_001.tif\n", + "4 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_13_RFP_001.tif\n", + "5 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_14_RFP_001.tif\n", + "6 file:/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5/B10_01_4_15_RFP_001.tif\n", + " Width_OrigDAPI Width_OrigGFP Width_OrigMito Width_OrigRFP\n", + "1 1224 1224 1224 1224 \n", + "2 1224 1224 1224 1224 \n", + "3 1224 1224 1224 1224 \n", + "4 1224 1224 1224 1224 \n", + "5 1224 1224 1224 1224 \n", + "6 1224 1224 1224 1224 " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "# Read in CSV files\n", + "qc_df <- read.csv(path_to_qc_results)\n", + "\n", + "dim(qc_df)\n", + "head(qc_df)" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Generate data frame removing poor quality sites and counting the number of sites left per well" + ] + }, + { + "cell_type": "code", + "execution_count": 4, + "metadata": { + "vscode": { + "languageId": "r" + } + }, + "outputs": [ + { + "name": "stdout", + "output_type": "stream", + "text": [ + "[1] 86\n" + ] + }, + { + "data": { + "text/html": [ + "\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\n", + "\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\t\n", + "\n", + "
A tibble: 6 × 2
Metadata_WellFlaggedFOVs
<chr><int>
B1 1
B100
B110
B121
B2 1
B3 2
\n" + ], + "text/latex": [ + "A tibble: 6 × 2\n", + "\\begin{tabular}{ll}\n", + " Metadata\\_Well & FlaggedFOVs\\\\\n", + " & \\\\\n", + "\\hline\n", + "\t B1 & 1\\\\\n", + "\t B10 & 0\\\\\n", + "\t B11 & 0\\\\\n", + "\t B12 & 1\\\\\n", + "\t B2 & 1\\\\\n", + "\t B3 & 2\\\\\n", + "\\end{tabular}\n" + ], + "text/markdown": [ + "\n", + "A tibble: 6 × 2\n", + "\n", + "| Metadata_Well <chr> | FlaggedFOVs <int> |\n", + "|---|---|\n", + "| B1 | 1 |\n", + "| B10 | 0 |\n", + "| B11 | 0 |\n", + "| B12 | 1 |\n", + "| B2 | 1 |\n", + "| B3 | 2 |\n", + "\n" + ], + "text/plain": [ + " Metadata_Well FlaggedFOVs\n", + "1 B1 1 \n", + "2 B10 0 \n", + "3 B11 0 \n", + "4 B12 1 \n", + "5 B2 1 \n", + "6 B3 2 " + ] + }, + "metadata": {}, + "output_type": "display_data" + } + ], + "source": [ + "# Count the number of flagged image sets\n", + "flagged_image_sets <- sum(qc_df$Image_Quality_Control_QC_Flag == 1)\n", + "\n", + "well_flag_counts <- qc_df %>%\n", + " group_by(Metadata_Well) %>%\n", + " summarise(FlaggedFOVs = sum(Image_Quality_Control_QC_Flag))\n", + "\n", + "# Print the number of image sets flagged and not processed\n", + "print(flagged_image_sets)\n", + "# Print the table\n", + "head(well_flag_counts)\n" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Generate bar chart to show how many FOVs were flagged after QC" + ] + }, + { + "cell_type": "code", + "execution_count": 5, + "metadata": { + "vscode": { + "languageId": "r" + } + }, + "outputs": [ + { + "data": { + "image/png": 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+ "text/plain": [ + "plot without title" + ] + }, + "metadata": { + "image/png": { + "height": 360, + "width": 840 + } + }, + "output_type": "display_data" + } + ], + "source": [ + "# Extract the first letter from 'Metadata_Well' to create a color palette\n", + "unique_starting_letters <- unique(substr(well_flag_counts$Metadata_Well, 1, 1))\n", + "\n", + "# Create a color palette based on unique starting letters\n", + "color_palette <- colorRampPalette(brewer.pal(8, \"Set1\"))(length(unique_starting_letters))\n", + "\n", + "# Map each unique starting letter to a color in a named vector\n", + "color_dict <- setNames(color_palette, unique_starting_letters)\n", + "\n", + "# Create a new column 'Color' in the data frame based on the starting letter\n", + "well_flag_counts$Color <- color_dict[substr(well_flag_counts$Metadata_Well, 1, 1)]\n", + "\n", + "# Increase the figure size to extend the chart horizontally\n", + "options(repr.plot.width=14, repr.plot.height=6)\n", + "\n", + "# Create a bar chart using ggplot2 with the 'Color' column for colors\n", + "fov_chart <- ggplot(well_flag_counts, aes(x = Metadata_Well, y = FlaggedFOVs, fill = Color)) +\n", + " geom_bar(stat = 'identity') +\n", + " labs(x = 'Metadata_Well', y = 'Number of Flagged FOVs', title = 'Count of Flagged FOVs per Well for Plate 5') +\n", + " scale_fill_identity() +\n", + " theme_bw() +\n", + " theme(axis.text.x = element_text(angle = 45, hjust = 1))\n", + "\n", + "# Save plot to qc_figures\n", + "ggsave(\n", + " barchart_output_file,\n", + " fov_chart,\n", + " dpi = 500,\n", + " height = 6,\n", + " width = 10\n", + " )\n", + "\n", + "# Display the plot in the notebook\n", + "print(fov_chart)\n" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Generate plate map to show distribution of flagged FOVs from QC" + ] + }, + { + "cell_type": "code", + "execution_count": 6, + "metadata": { + "vscode": { + "languageId": "r" + } + }, + "outputs": [ + { + "data": { + "image/png": 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xxdi7RGOJRk1iL2CLNFHsDaWJFOnsUpYtbJl5/9jvj9eLypZ5dp1rzucvwdkz52FnZ2fOPKXvb7/95ufnN2nSpIcPH5o6SSry8/MnTZpkiWsOr/o+tleYPnfpOI7H9gr7ffViS1wzadKk/Px8M+RptIcPH8bFxfkKbX9bsUifah2GYQwGPrJf399XL26qqfz888/LyspMnSQV169f//zzz/u42+6bMlKfah2GYWwmc3zPkENffvTi4d1p06aJRCJTJ0lFcnLyjBkzRnXy3jVpmD7VOgzD+Bz2lKiu+6eOvH45KT4+XiKRmDpJKv7444+5c+dOmzZN/wcYVlZWs2bN2r1796lTp+bPn9/c3GzqJKlITEz8z+LvFw3utfLjD/Sp1mEYZm/BTxjcZ8OYAXt2bl++fLlGozF1kkYjCGLt2rWbNqz/afbURVMm6FOtwzDMxdH+h5mf/zAjbvWqlVu2bIG+HfTBYrH27NmzZMmSkpKSd50LAMBMoGAHADDSxo0bs66n7/9xfs/OHQ197dDInrsXzz1y+NCRI0dMkRt1JEkuX7686PH9g9NGBbsbPA/6+J4h6z/5YMvGDbQtaWk0moULFyqe3D3ow/F90xjYNuAYNsORtUxILl++/ObNmybKkKLm5uY5c+bYFOfs9X7zGNg2MDEswZkVb6VMSEh4+vSpiTKkqLGxcdasWQEBATt37jS0wwKXy12xYsWYMWO++eab4uJiE2VIUU1NzezZsyNDOmz6Lt5SYFhPZEsBf9N38ZEhHWbPnl1TU2OiDCkqLi7+5ptvRvfrveLrydw3DRFtg72N1a7FcwJcHWfNmkXbik92dnZCQkJ8dNeEwX2Y+j0PaOFma7V38r9slZI5c+bQtuJz48aN5cuWLh3ed3qM7r6frfgK7Q5O+0hRUbJw4UKCIEyUIUXJycmbN29ev379p59+auhrQ0JCDh48WFRUtHz5ctpWfI4cOXLk4IFdk4YN6exv6Gt7+nnsnzLydtq1TZs2mSI3JHbu3Hnt8qV9y+dHdjN47uCBPbvv+c+8s6dO7tu3zxS5/UO87cxgdC/RsLCwyZMnz5gxo2WVM22P2pZPWWNjo3GRAQD0BAU7AIAxzp49e/rkyY3zvvJyMXJRvxB/3zWzp23ZsoWenZj27dt3My1lw6cfOlm/YTIpffRq77l8VP8ff/wxOzsbbW5IbNy4sfhu1np3lo2xY6dirRjxdsT8+fMLCwuRpoYASZI//vij4nnOane2wMBKQYsxdszxlpq5c+dWV1ejTY86giCWLFliZ2e3bNky4wYw4jj+5ZdfDhw4cM6cOTSs+CiVynnz5vm7OX0XN45h1DvIYODfxY0N8nSdM2eOQqFAniFFUqn0u+++i+kWPH30cOPu3Dhs9vKvvnC04C5YsICGfXxqa2u/++67CeEdx4QbOc+XgMNe88kAZW3lf/7zHxpWfIqLixctWjRrQHhsJyPn+bLhczeNH1Sam03Pik9OTs7KlSsXL17cq1cv4yI4OTlt3rz51q1b+/fvR5oaGllZWVu2bPnxo36dPZ2Ni+DlYLN5/KAzJ/46ceIE2tyQuHLlyu+HD/08Z7qfh5FTHwS281o3d8aeX365cuUK2tz+Oci3MzrmihUrcnJykpKStD+6uLiw2ezHjx9jGJabm3v79m00qQMA6AEKdgAAg1VWVq5evXrZjDh/Lw8qcSJCgr4e868ffvhBJpOhyg2JvLy8xF27fh4b62FHadmBDzq2G9s9cMmSJWq1GlVuSNy6devksaMbPNj2LEozAY23Y/ZjKVasWEG32+lLly5lXkre5MGypPYtN9ORGagQr1mzBlFeyBw9erSgoGDdunUUpxv77rvvbG1tN2/ejCoxVH755Rd5g3jNv6cZV63TYjAYK+KnNEsafv31V4S5IbFhwwZLJrZoygQqQVhM5prZ017k5/7555+oEkNl5cqVgXb8Gf3CqAQRcNgbPv3wdmbG5cuXUSWGBEmSS5cu7dfe7dMISvN42vC5P4+J/euPY3fu3EGVGxJqtXrRokVjxowZPHgwlTiurq4//fTT7t276TY4XSaTLVmy5Ot+3fv6U1r03Fdot3REzLp16yorK1HlhoRIJFq+fPn3UycGt29HJU6XAL9vP/tk1apV0G+LPgQCwa5du/Ly8rQ/8vn8devWxcXFffDBB+vXrx81ahTdrjkBAFRAwQ4AYLCdO3dGBHfoH96Veqjxgz9wtBIcOnSIeiiEtm7dOrSLv54zLrVtRr8wZYP45MmT1EOhQpLk1q1bx9uz/LkI5u2e68QsevLo2rVr1EOholKpdu7cOVXIMnQk7OsYOJbgzLqZnk6r22mZTLZ///5Zs2ZRX2KFzWYvWLDg3LlztOomWVNTc+TIkXmTxgooL+8g4HG//eyTw4cPV1VVIckNiaKiovPnzyd8Po79/8Y0Gc3exjp+3Kg9e/Y0NTUhyQ2J+/fv38zM/PbD3q8vtWkoN1urKZHdtm3bplQqkeSGxNWrV4sL8v89EMGyQgEuDuPCgzds2ECrgbEnTpxQKBTTpk2jHqpbt26DBw/eunUr9VAIHTp0yJ6Fje+JYN2k/kHteng779q1i3oohBITEwO93Yf0jaAe6qMPIn1cHOnZTfKfo7KyMizs/3/+ERsbS5Jky9PE2bNn5+fnX716NTEx8eDBg8uWLfPw8CBJ0tbWtuUf7yhxAABVULADABimsLDwYnLy12NHIonGYDC+GjPi0KFD9fX1SAJSd+/evXu3b02PodQxpAWHxfwyuvsvv/xCn2mYrl69Wpb7bJI9mvO/NROf5MDavn07fe42T548SVSVjbZB00A3Nj7alrFz504k0ZD47bffhEJhbGwskmgBAQGxsbG0auCePXu6+LcLDw5EEq1n546hAb606mS3Y8eOgRHdAttR6trTYlDvHk42locPH0YSDYlt27aNCe+o5xT+On3SoyMmk5w6dQpJNOoIgtixY8fnfUNt+GhWsP0iMrSytJg+jz2am5v37t07Y8YMVAsiz5gx4+7du/fu3UMSjbqGhoZDhw7FDwinXlDWmjUgIjk5mT6PPV6+fHnmzJlvJoxGEg3H8a/Hjjx69CgNZ4cAAID3HhTsAACGSU5ODg8ObO/pjipg364hznY2KSkpqAJSdOHChdjg9kZPXfe6YaEBWLM8KysLVUCKLly4MNKWacVEc6OCYdgYO0ZFSTF9FmdISkoaY8fkUBhK2cpEe+bjRw/psxznhQsXJkyYoOeasPqYOHFieno6TWayU6vVly5d+mzoQIQxPxs68NKlSzQZJSSRSNLS0iaiayCDwZg4dOCFCxdQBaSorKzs8aNHE3oaPMn923BYzLHhwfRp4NOnTyvLyz7pYfBqS29jxeOO7Bp4/vx5VAEpysrKIghi2LBhqAI6OzvHxsbS5x1MSUlxEnAoDoZ9VXsn+4h2rvRZY+rSpUsh7X06+nqjCtg9KKC9h+vVq1dRBQQAAKAnKNgBYIy5c+f+6zX/kEuZlJSUmLBQtDFjwrqkpqaijWkcgiDS09NjAn0QxmTgeGSAF00qkgqF4tatWzFWKE/+AgYeYcGgyTsoFouzs7OjKM5d99+c2HgQF6dJA/Py8qqqqvr27YswZkBAgJOTU0ZGBsKYRrt79y6pUXfvGIAwZlinDhihoUkHn4yMDKGtTYA3pQlAW4nsGlJVWUmTacJSUlI6ugkRPvPAMKxfkE92djZNOvikpKT09PMQ6LewL/6v6fpsFhPok5WVJZfLqaWGRkpKSlRUlJ6PBPRcMiUmJiYtLY0mHbFTUlL6Beo7s5u+72AHH5p8y2OGXKfhHXroGTMmLJQmX4IAAPCPQnXyFAD+sWJiYj799NNXf/NPmCGivLz8xYsXUfN0XL++fglI5ra1alVUt86HV25SKpUUZ9Cn7tmzZ00N9RG+OvoPvn4FT57Z3cb20YE+Ky7Sohpy+/ZtgUoRzNMxkgs/nNnqN+SE3m1sH2XJOJae/vXXX1PNj7KMjAwfNubFaese8vXWYboaGG3FzMjIGD9+PNX8KLt+/XpoaKi1tV7LoeA4rud6IFFRURkZGRQnmEfi+vXrvbt00nNyN7xDj7bPLVosJrNX544ZGRnh4eGUE6Tq+vXrUd0761nm0LOBVhaCroH+GRkZ/v7+lBOk6vr161Ed9O3ag/9retsnTy03Wytfoe2NGzdGjBhBLTsEMjIyxgbq1TlLz1oPhmHBHk4WLMadO3ciIyMppIZGRkbG999/r8+W+i9wHBERIZVKnz171qmTkasGo6JUKm/duvXFhEH6bKz/OxjVwWfV+Yzy8nI3NyOXZEVFLBY/e/Zs9Zf6flvpeZKJ6t559/FzUqnUwgJlLR4AAEDboIcdAEaysLBw/W98Pv9dJ2VyBQUFQjsbR1sbtGE7+Hiq1arS0lK0YY2Qn5/fTmjHYyN+mBHo4igWi8ViMdqwRsjPz7+AM7QAACAASURBVA/kU1l4882CeIyioiI6DDksKCgIQjPt0n8J5OIFBQXo4xquoKCgY0fdY/FwHNfeS7f8o21BQUHPnz9HkB9lBQUFQe10l3vwDj20DwZa/tG2QB8vGr2DegxVM7SBQb5eNHoHXR11bob/a7q2GtLyj7YFuQrp0ECNRlNUVNTR3Un/l+jTOgaOB7o60uEQ1X5VBQUF6dyy5cSizxmGx+O1a9eODg0sLS1VKZWB+h2irf7RBgdLvtBKQIcGFhQUWAkE7k56NPD/nVj0OcP4ursyGfiLFy+o5gcAAMAQ0MMOAJMgCCI3N7flR4lEwuFwWpUzCIIgSdLMNQ6Ku6uqqnJxsEeVTAsOm+1gY1NeXu7tbfyUKyRJUv97VlZWutqimSj9VUIrAYvBqKio0LNj1BshOWCqqqpcTHDid2XjarW6urraycmA+9hWNBoNkga2o7w47OvcOLj4pVgul7PZeo2DeyONRkMQBMUGVldXd+nSxdBX6exq5+rqWlVVRTE3giCoN7Cqqsqlp44Gvn57qbOTiJuTQ+X1O9QbiFE+i1ZXV7s46jiL6j9OrYWLg/2jBznUG6jRaKgEUavVYrHYxcay7c3077jUwtXWqqCigmIDSZLUaDT69wt7XXV1tVqt1tlAzPA2uthYVlZWImmgnv1q36iyspLJZAqFQippvJGrq2sFindQrVZTeQcrKirsLfgcFpNKGm/kiuIdxCifYSorK10cqS4g/joGg+HiYF9RUREYSHU5IHNe977xnKa9mjJbDgAAQAUU7AAwCbVa/dlnn7X8GBoa2rt37zcuhGrmOWsoLsYqFov5ukZTGkfA49bW1lJfK5ZihIaGBj7q7nUYhuE4LuCyq6urnZ2dKYai2MDGxkYn5P3rMEzAwDAMq6qqoj6oWalUUnl5Q0MD3yQNxDEMq6ystLKiWs+l2ECJRKKzM68Rd7MWFhYymQzJYs0KhYLKy6VSqcAEJxkBj4eqgTKZjMrL5XI5n2twA3VWJC34vMbGRuoNpLiedVNTE4ZhAq7B5wGdY2MFHFajGEEDKX4Aa2pqMAzT+TXRqlqnz8hfCy6nvL6eegMbGxupvLympobP5+s8h7TaQJ/R9wKBoK6ujnoDGxoaqLy8traWr8f8g8a9gyKR6J1fxojFYn3OMK2eCugzMFbA59HhOs0IrU7aCoWCDgMCAABAH1CwA8BIFy5cSEpKevU369ata9++vfbfTCYzLi6u5b9EIhGbzW51m629XGDpN1UTEgqFgsejNFzQwsJCZZqrHKVKbWFhQXFYMfUG8vn8OrWGSoS3aVapLS0tqTSQJEmlUsk1/Fb/VXw+X2WC58ramFZWVlQaqO2cRbHkx+fzlQT6FipJEsMwa2trKgeYRqPRaDQUG8jj8VQqFZUIb9Tc3MzhcCh+ANVqNUmSVDohYhjG5XKVKvQnGaVKRb2BKpUKx3GKJ202m22Ks2izUsXn86k3kMFgMJnGdz7SrlSgNLyBOqshSjXB4/EoNlCpVLJYLCorLFtaWmIYptIQhnbR0lnxaVapBQ4Cig3UfpCpdECztLQ0rqaps2anVCrt7e3f+be8paWlSmOab3k1LS5jTHmdpqJ4GYOhaKBB3nhOY7PZVM5yAABgTlCwA8BIkZGRn3zyyau/cXV1bfk3k8mcNWtWy48HDhzgcDitZupVKBQkSZpz5rvm5maKswU7OTmJGySo8mlBEIS4sdHV1ZVKetp6FsUGCoXCJ1L0fR6lzapmtcbNzY1KetpyD8UGOjo6lqjR17Nq1SSO425ubgKBwOggarVaLpdTb2CdCe7FRGqMz+c7ODhQCaJUKqkfovb29iKRqO1tSJJsdceus/OLSCSys7OjmJtCoSAIgsoxgGGYvb29uEFHFyEy93ar7iE6+4aI6hsdHBwoNlAmkzEYDIp3m3Z2dqJ6EzSwAUEDpVIpm82mUlPWFizEUoWnfVtTnZJndhs6YlQklTm6UjqFYhhGEASPx6NSU3Z1dcVxXNQks+C+tYFGDPjFMEwklXkLhRQbqFar+Xw+lWKEq6urUqlsamrSlibfyLiCoEgk6ty5M8UGKpVKgUBApSLp4uJSJ5UTJMl4e5A3voM6S65iqcLZ2ZliA6lfpzk7O4sbdVynvXHQvc5OdqL6Rjo00CBSqZTFYrV60slms6lU7QEAwJygYAeAkaysrKhMuPY/ytvb+2VVTbNSxdVjRIn+ymtESpWaDn9Pb2/vF7X1r9c7KHpeLeZwOK+WdN8Vb2/vFEoDwt6ssJkUCoUUKzVIeHl5pSsI5GGfNxPe3r7IwxrB29u7sLBQ52avHsP6TNZTWFhIhw8ghmFeXl7PX5br3OzVkpY+Sxw+f1nu5aXXyp6m5u3tXVhW3h/r2vZmhjaw8GW5t7/u1UjMwNvb+3m1uIunjuH/r9bs9Fko9nm1uE/Pd3+ICgQCoVBYWFPn5fDWgp0+zXldYU19FA0+gy4uLhwOp7CwsHPnzm/bxoj5v0iSfPHixeeff04pORS8vLyUGqKsrrGNmrIR76BSrXkpbqTDWdTb27tKVNckk1sK3vo8WJ9TSiuihsaGJqmPjw+l5AAAABgICnYAAAP4+/tb29hkPX4W1f2tl/KY4deCGfcfBwYGUlmQAZXQ0FCpSvO0vLaTe1tTbht6NZ+RXxIWFkaHJ7rh4eE/qLByFenW5soM5ITeBoXNkBIRkRHUUkMjIiJi+xayUUNaM9/aQENbh2FYRhMRHhtOLTU0wsPDFy1apFardQ7MNOimOiMjo3///tRSQyMiIiJx+1Z9tjToPHP9wZPp8bONTQql8PDwvy+cmTpqqM4t9W+gWqPJepyzcsLnlDJDJDw8PPNW2kfddS8zqv+JtKlZ+ai0em4ELU4y4eHhmQXPYwJ9EMYsr5cU1db36GHwYiPIMRiMsLCw69evt1GwM0J2drZUKg0NDUUY0zjW1taBgYHX80vHRaBc7/7m85cCS0t/f3+EMY3j5eXl5OSU+TA7tlcYwrAZ9x9rIyOMCQAAQKd3f/cIAPgfwmAw+vbtm3r3IdqwqXcfRUdHo41pHIFAEB4enppbhDZsam5RTEwM2pjGEQqFgYGBqRKUfdAIEktv0tDkHQwICHBydbshRTnst5nEbskImryD2lv6+/fvI4wpFouzs7Np8g727du3olasTyc7/RWUllXUivv06YMwptFiYmKeFBTV1lOaOL+Ve8/yNRgWFoby/txo0dHRNwpKFUgnIryeX+ogFAYEBCCMabTo6OiUnCIC6SqTKTlFQUFBNKmGxMTEpKSkoI2ZmpoaHh5Oh17YGIZFR0en5hajjZmaWxQdHU2HmdFwHI+OjjbBddrDfv36oY0JAABAJyjYAWCMDRs2TJ9uzCQ174H+/ftfu/1AIqW0TuKrXlbV3M/Jp8+FYP/+/ZMeFyjRLT3xtLymWCyJjIxEFZCi/v37n23QIFyY4bqUkHMEEfTo/IJhWExMzOl6lMWCi42ElYMwODgYYUyjsVisPn36nDlzBmHMM2fO+Pn50WTEqLW1dffu3U9fu44w5pmUzLCwMBsblB1qjObh4dG+fftzaTcQxjybmhkZGUlxuQ9UQkJCrO3sLz15jjDmmfs5/fv3RztTgdF69uypIPHr+SWoAhIkee5hHk26uGIYFhUVVVxcnJ2djSqgUqlMTk6mTwP79+9/v7iiVIysaC5RNF/LKaLVZUz6vcd1umay01+1uP7mo6f0eQcBAOCfAwp2AADD9OnTx9vX98DZS6gCbjt6Kjomxs/PD1VAigYPHsy0tPnrzlNUAbdeuTVq1CihsK0xtuY0ZsyYWgu7ixI0FUkCw7bVqCdOnGjO5VPa9tlnnz0meVlSNL0IlSS2u0Y9ZcoUOoxo1po6deqVK1dycnKQRGtsbDx48OC0adOQRENi2rRpf15OLauuRRKtvEb015W0L7/8Ekk0JKZOnXrg7KWGJimSaHnFLy9n3Zs8eTKSaNQxGIzJkyfvSrmDqpPdzecvH5TVTpgwAUk06vh8/sSJE7dfvY2qk13y44JqhWb06NFIolHn6Og4cuTIzZs3owr4xx9/4Dg+aNAgVAEp8vX1jYqJ2XntDqqA+9IfuHh606QPL4ZhYWFhHYOD955KQhVw55+nw3qEd+rUCVVAYJDw8PBly5a1/Lhx40YOhyOR/F9BliRJV1fXffv2DRs2DH8NTZ41AgCMRpfbDwDA/wocx7/66qsjyVfLa3QsVamPh3nPU+89+vrrr6mHQoXNZs+cOXNv2r06FMvFpuQUZVfV0aoaIhAIvvjii501GhmKXnan6ol6S/uJEydSD4WKUCj89NNPN1erVSjupg+KNFw3z5EjRyKIhYiPj8+QIUO2bNlCEAiKkrt37/b29qZP3xAMw7p27dqrd+/tx04hibb1yIlevXvTYfKsFv369fPx9Us8fo56KIIgNh76a/jw4b6+tFgURWvUqFE8W4ffbz6mHkql0Wy5kjVhwgRnZx2rWJjTxIkTxWrs9D0ERXOZUrXz2p2pU6e2sSqr+X355Zc5OTmpqanUQ9XV1f3666/x8fFUVh9GLj4+/lpuyaOXVdRDldVJjt16MmfOHPo81MEw7Kuvvjp+Ja2ovJJ6qJwXJUkZt2bNmkU91D/T60U0QzsLDx8+/MKFCy0/JiUlOTg4XL58WfvjgwcPqqurhw4deu7cOZIkSZL89ttvR4wYof33kydPUDYGAGB2NPpqAQD8r+jRo0f/DwZ8u36nTKGgEqemrn7hll/i4uJoMhavRWxsbFCXrvP/vKLSUOqGViJqWH4mNT4+3t7eHlVuSIwePdrWP+g/FWqKJbunCnJ9lWrevHk0mZmoRVxcnMLZc20V1Q4+WVLiV5FmwYIFOld4MLOvvvrqxYsXu3cbsxjlqy5dunTq1Kn58+fTZLBhizlz5tx4kns0+W+KcY4k/531NH/OnDlIskIFx/GEhIRTKZmXblDt47Prr7PFNeKZM2ciSQwVFou1YMGCPWn3sgrLKIb66cL1Zq4lHVYXfZVAIJg3b976izeeltdQiUOQ5JITf9u7e3388ceockPC3t7+66+//uGHH0pKKI38ValUCQkJnTp1GjhwIKrckPDy8po0adKCP6/USCh1dJUpVfOOXfog9kM6LBjyqs6dOw8bPnzehl0UJzCpa5QkbNo9dtw4Oqyn8Y81fPjwO3fu1NTUYBgmk8kyMzPj4+OTkv6vB2VSUlJ4eDhNZsAEACAHBTsAgDEWL17MtrResn2f2tiSlkyhmLdhV1BIF1oNVdPCcXzVqlW1JHvN+QyjBz3VyxRzjiT3ix00ZswYtOlRx2az161bl81z2F5jfEmrSk3OK1NN+GIy3e7EMAyzsLDYuHHjFY3gsNj4kmthM7GwXB0/Z054OC3Wh32Vg4PDunXrDh069OpTd0M9fvx4+fLlS5YsCQwMRJgbEp6enqtWrdpy5GTmQ+Mn0sp8mL31yMlVq1Z5eHggzA2JoKCgJUuWLE88+Di/0OggFzKyDif9vW7dOro9EsAwLDw8PH72vxf+daWwps7oIL/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+ "text/plain": [ + "plot without title" + ] + }, + "metadata": { + "image/png": { + "height": 360, + "width": 840 + } + }, + "output_type": "display_data" + } + ], + "source": [ + "# Add genotype to data frame to label on plot\n", + "well_flag_counts <- well_flag_counts %>%\n", + " mutate(\n", + " Metadata_genotype = case_when(\n", + " as.numeric(gsub(\"\\\\D\", \"\", Metadata_Well)) %in% 1:4 ~ \"WT\",\n", + " as.numeric(gsub(\"\\\\D\", \"\", Metadata_Well)) %in% 5:8 ~ \"HET\",\n", + " as.numeric(gsub(\"\\\\D\", \"\", Metadata_Well)) %in% 9:12 ~ \"Null\",\n", + " )\n", + " )\n", + "\n", + "fov_platemap <- platetools::raw_map(\n", + " data = well_flag_counts$FlaggedFOVs,\n", + " well = well_flag_counts$Metadata_Well,\n", + " plate = 96,\n", + " size = 8\n", + " ) +\n", + "\n", + " ggtitle(paste(\"Platemap of Flagged FOV Count Per Well in Plate 5\")) +\n", + " geom_point(aes(shape = well_flag_counts$Metadata_genotype)) +\n", + " scale_shape_discrete(name = \"Genotype\") +\n", + " scale_fill_gradient(\n", + " name = \"Flagged FOV Count\",\n", + " low = \"white\",\n", + " high = \"red\",\n", + " ) +\n", + " theme(\n", + " plot.title = element_text(size = 10, face = \"bold\"),\n", + " legend.text = element_text(size = 8), # Adjust the text size\n", + " legend.title = element_text(size = 8), # Adjust the title size\n", + " legend.key.size = unit(0.5, \"cm\") # Adjust the key size\n", + " )\n", + "\n", + " ggsave(\n", + " platemap_output_file,\n", + " fov_platemap,\n", + " dpi = 500,\n", + " height = 3.5,\n", + " width = 6\n", + " )\n", + "\n", + "# Display the plot in the notebook\n", + "print(fov_platemap)" + ] + }, + { + "cell_type": "markdown", + "metadata": {}, + "source": [ + "## Count of flagged FOVs based on site number" + ] + }, + { + "cell_type": "code", + "execution_count": 7, + "metadata": { + "vscode": { + "languageId": "r" + } + }, + "outputs": [ + { + "data": { + "text/html": [ + "\n", + "
  1. 86
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625 rows × 8 columns

\n", "" ], "text/plain": [ - " Metadata_Frame Metadata_Series Metadata_Well Metadata_Site \\\n", - "9106 0 0 D1 4 \n", - "5778 0 0 D6 16 \n", - "42 0 0 B11 2 \n", - "5273 0 0 B6 15 \n", - "5983 0 0 E4 11 \n", + " Metadata_Frame Metadata_Series Metadata_Well Metadata_Site \\\n", + "9106 0 0 D1 4 \n", + "5778 0 0 D6 16 \n", + "42 0 0 B11 2 \n", + "5273 0 0 B6 15 \n", + "5983 0 0 E4 11 \n", + "... ... ... ... ... \n", + "20978 0 0 E7 2 \n", + "22921 0 0 F1 1 \n", + "6703 0 0 B1 25 \n", + "22828 0 0 E9 2 \n", + "20841 0 0 E2 15 \n", "\n", - " ImageQuality_PowerLogLogSlope ImageQuality_PercentMaximal Channel \\\n", - "9106 -3.932344 0.000090 GFP \n", - "5778 -3.817712 0.523093 DAPI \n", - "42 -3.744752 0.760690 DAPI \n", - "5273 -3.668699 0.744151 DAPI \n", - "5983 -3.609106 0.192319 DAPI \n", + " ImageQuality_PowerLogLogSlope ImageQuality_PercentMaximal Channel \\\n", + "9106 -3.932344 0.000090 GFP \n", + "5778 -3.817712 0.523093 DAPI \n", + "42 -3.744752 0.760690 DAPI \n", + "5273 -3.668699 0.744151 DAPI \n", + "5983 -3.609106 0.192319 DAPI \n", + "... ... ... ... \n", + "20978 -0.458892 0.000090 CY5 \n", + "22921 -0.358638 0.000090 CY5 \n", + "6703 -0.307717 0.000090 GFP \n", + "22828 -0.209380 0.000090 CY5 \n", + "20841 -0.192534 0.000090 CY5 \n", + "\n", + " Metadata_Plate \n", + "9106 Plate_3_prime \n", + "5778 Plate_5 \n", + "42 Plate_3 \n", + "5273 Plate_5 \n", + "5983 Plate_5 \n", + "... ... \n", + "20978 Plate_3 \n", + "22921 Plate_3_prime \n", + "6703 Plate_3 \n", + "22828 Plate_3_prime \n", + "20841 Plate_3 \n", "\n", - " Metadata_Plate \n", - "9106 Plate_3_prime \n", - "5778 Plate_5 \n", - "42 Plate_3 \n", - "5273 Plate_5 \n", - "5983 Plate_5 " + "[625 rows x 8 columns]" ] }, "execution_count": 5, @@ -716,7 +803,7 @@ "\n", "print(blur_outliers[\"Channel\"].value_counts())\n", "# Print example outliers to visualize\n", - "blur_outliers.sort_values(by=\"ImageQuality_PowerLogLogSlope\").head()" + "blur_outliers.sort_values(by=\"ImageQuality_PowerLogLogSlope\")" ] }, { @@ -937,7 +1024,7 @@ "- Smudged images or images containing large artifacts (usually seen in `DNA` channel regardless of stain)\n", "- Overly saturated channels which can occur in any channel, but specifically we are seeing `Mito` having the most problems with this.\n", "\n", - "This means that we will only be setting threshold for outliers for all channels just like for blur." + "This means that we will be setting a threshold for outliers for all channels just like for blur. There will be only one threshold for images that are above the mean (**2 standard deviations**) since we are trying to remove over-saturated or images contain artifacts." ] }, { diff --git a/1.cellprofiler_ic/image_quality_control/2.qc_report.ipynb b/1.cellprofiler_ic/image_quality_control/2.qc_report.ipynb index 88043a4..f865df3 100644 --- a/1.cellprofiler_ic/image_quality_control/2.qc_report.ipynb +++ b/1.cellprofiler_ic/image_quality_control/2.qc_report.ipynb @@ -410,10 +410,7 @@ " dpi = 500,\n", " height = 6,\n", " width = 10\n", - " )\n", - "\n", - "# Display the plot in the notebook\n", - "print(fov_chart)\n" + " )\n" ] }, { @@ -487,10 +484,7 @@ " dpi = 500,\n", " height = 3.5,\n", " width = 6\n", - " )\n", - "\n", - "# Display the plot in the notebook\n", - "print(fov_platemap)" + " )" ] }, { @@ -537,6 +531,21 @@ }, "metadata": {}, "output_type": "display_data" + }, + { + "data": { + "image/png": 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+ "text/plain": [ + "plot without title" + ] + }, + "metadata": { + "image/png": { + "height": 360, + "width": 840 + } + }, + "output_type": "display_data" } ], "source": [ @@ -548,7 +557,7 @@ "# Example using viridis palette\n", "site_FOV_plot <- ggplot(flagged_qc_df, aes(x = factor(Metadata_Site))) +\n", " geom_bar(fill = \"steelblue\") +\n", - " labs(title = \"Count Plot of Site Integers\",\n", + " labs(title = \"Count of Flagged FOVs per Site Integer in Plate 5\",\n", " x = \"Site\",\n", " y = \"Count\")\n", "\n", diff --git a/1.cellprofiler_ic/image_quality_control/qc_figures/Plate_5_per_site_flagged_fov.png b/1.cellprofiler_ic/image_quality_control/qc_figures/Plate_5_per_site_flagged_fov.png index af74eb948cf7be01d3077fc308e228fe98fa584e..830fb2630c941d6c7c6d894be7b3b448258b8d6d 100644 GIT binary patch literal 112169 zcmeFZgiS_bI00ypYM3S^WFOw+;x8E9DgiW>wTZ+nRAXg#+Yw_iqeIBB=jUiL`3^8%AHds zBBHoQL`3>}&mZ`SUv6y-{%@DD!i950TZI24mZyc|C&V}8t~e4ADUJ~SyUn?2CX$Hg 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zJoMbraxPZbi3HCV%}flwyz;x*^y+_R8Cr{qp1EWj&vZdu0ME`023%}o0M(kzz@dl| z%TG>DPErz)o=`SH=50d{LC`W<_$kWOJ@RODv6=H3S`~JCtQZihrWjg*Gm?ztqV;l? zdyn6sc}0ZuLeYc>(*`dI@8B3o{ZLwdA}34`?gYMIl*W|Q)LCK?l*(JQ<7+~;5->!f zNk{Hu;B3<-=X+u{HlEJ=103f9oj>&2(3bqUKg|7-WBlh{&;1SU|4lgl_a(SD|Gh6H kK$Q9a8w~$XgJEErhC8S(+p>&yAG!Zp+xMtvs9Fa64=^tss{jB1 diff --git a/1.cellprofiler_ic/image_quality_control/scripts/1.evaluate_qc.py b/1.cellprofiler_ic/image_quality_control/scripts/1.evaluate_qc.py index 4842b5b..8d9b6e6 100644 --- a/1.cellprofiler_ic/image_quality_control/scripts/1.evaluate_qc.py +++ b/1.cellprofiler_ic/image_quality_control/scripts/1.evaluate_qc.py @@ -84,12 +84,12 @@ for plate, qc_df in all_qc_data_frames.items(): plate_df = qc_df.filter(like="Metadata_").copy() - # Add PowerLogLogSlope column + # Add PowerLogLogSlope column (blur metric) plate_df[f"ImageQuality_PowerLogLogSlope"] = qc_df[ f"ImageQuality_PowerLogLogSlope_Orig{channel}" ] - # Add PercentMaximal column + # Add PercentMaximal column (saturation metric) plate_df[f"ImageQuality_PercentMaximal"] = qc_df[ f"ImageQuality_PercentMaximal_Orig{channel}" ] @@ -149,7 +149,7 @@ print(blur_outliers["Channel"].value_counts()) # Print example outliers to visualize -blur_outliers.sort_values(by="ImageQuality_PowerLogLogSlope").head() +blur_outliers.sort_values(by="ImageQuality_PowerLogLogSlope") # ### Identify threshold values to use to find outliers above and below the mean @@ -309,7 +309,7 @@ # - Smudged images or images containing large artifacts (usually seen in `DNA` channel regardless of stain) # - Overly saturated channels which can occur in any channel, but specifically we are seeing `Mito` having the most problems with this. # -# This means that we will only be setting threshold for outliers for all channels just like for blur. +# This means that we will be setting a threshold for outliers for all channels just like for blur. There will be only one threshold for images that are above the mean (**2 standard deviations**) since we are trying to remove over-saturated or images contain artifacts. # In[10]: diff --git a/1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.py b/1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.r similarity index 87% rename from 1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.py rename to 1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.r index bf84471..5667358 100644 --- a/1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.py +++ b/1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.r @@ -1,13 +1,3 @@ -#!/usr/bin/env python -# coding: utf-8 - -# # Whole Image Quality Control Report - -# ## Import libraries - -# In[1]: - - suppressPackageStartupMessages(library(dplyr)) suppressPackageStartupMessages(library(ggplot2)) suppressPackageStartupMessages(library(platetools)) @@ -15,12 +5,6 @@ suppressPackageStartupMessages(library(arrow)) suppressPackageStartupMessages(library(RColorBrewer)) - -# ## Set paths and variables - -# In[2]: - - # Paths to CSV files to generate QC report path_to_qc_results <- file.path("../Corrected_Images/Corrected_Plate_5/IC_QC_RunImage.csv") @@ -33,24 +17,12 @@ # Output path for site bar plot site_FOV_output_file <- file.path(paste0("./qc_figures/Plate_5_per_site_flagged_fov.png")) - -# ## Load in outlier and quality control results - -# In[3]: - - # Read in CSV files qc_df <- read.csv(path_to_qc_results) dim(qc_df) head(qc_df) - -# ## Generate data frame removing poor quality sites and counting the number of sites left per well - -# In[4]: - - # Count the number of flagged image sets flagged_image_sets <- sum(qc_df$Image_Quality_Control_QC_Flag == 1) @@ -64,11 +36,6 @@ head(well_flag_counts) -# ## Generate bar chart to show how many FOVs were flagged after QC - -# In[5]: - - # Extract the first letter from 'Metadata_Well' to create a color palette unique_starting_letters <- unique(substr(well_flag_counts$Metadata_Well, 1, 1)) @@ -105,11 +72,6 @@ print(fov_chart) -# ## Generate plate map to show distribution of flagged FOVs from QC - -# In[6]: - - # Add genotype to data frame to label on plot well_flag_counts <- well_flag_counts %>% mutate( @@ -153,12 +115,6 @@ # Display the plot in the notebook print(fov_platemap) - -# ## Count of flagged FOVs based on site number - -# In[7]: - - # Replace 'path_to_qc_results' with your actual data frame name flagged_qc_df <- qc_df[qc_df$Image_Quality_Control_QC_Flag == 1, ] @@ -167,7 +123,7 @@ # Example using viridis palette site_FOV_plot <- ggplot(flagged_qc_df, aes(x = factor(Metadata_Site))) + geom_bar(fill = "steelblue") + - labs(title = "Count Plot of Site Integers", + labs(title = "Count of Flagged FOVs per Site Integer in Plate 5", x = "Site", y = "Count") @@ -180,11 +136,8 @@ width = 10 ) - -# ## Determine how many misclassified cells match with flagged images from QC (from training ML without QC) - -# In[8]: - +# Display the plot in the notebook +print(site_FOV_plot) # Load the parquet file into misclassed_df misclassed_df <- arrow::read_parquet("./misclassified_cells.parquet") @@ -204,4 +157,3 @@ anti_join(flagged_qc_df, by = c("Metadata_Well", "Metadata_Site")) dim(filtered_misclassed_df) - diff --git a/1.cellprofiler_ic/nf1_quality_control.sh b/1.cellprofiler_ic/nf1_quality_control.sh index 0b04ceb..64a2a6d 100644 --- a/1.cellprofiler_ic/nf1_quality_control.sh +++ b/1.cellprofiler_ic/nf1_quality_control.sh @@ -5,10 +5,24 @@ conda init bash # activate the main conda environment conda activate nf1_cellpainting_data +# go into the image_quality_control directory +cd image_quality_control + # convert all notebooks to python files into the scripts folder in the image_quality_control folder -jupyter nbconvert --to python --output-dir=image_quality_control/scripts/ image_quality_control/*.ipynb +jupyter nbconvert --to script --output-dir=scripts/ image_quality_control/*.ipynb + +# run the image quality control scripts (python) +python image_quality_control/scripts/0.whole_image_qc.py # Comment out unless you have made changes to the QC pipeline +python scripts/1.evaluate_qc.py + +# deactivate the main conda environment +conda deactivate + +# activate the R specific conda environment (from 4.analyze_data) +conda activate r_analysis_nf1 + +# run the image quality control R script +Rscript scripts/2.qc_report.r -# run the image quality control scripts -python image_quality_control/scripts/0.whole_image_qc.py -python image_quality_control/scripts/1.evaluate_qc.py -Rscript image_quality_control/scripts/2.qc_report.r +# return back to original directory +cd ...

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+Metadata_Tags,"[""ImageNumber""]" +Pipeline_Pipeline,"CellProfiler Pipeline: http://www.cellprofiler.org +Version:5 +DateRevision:424 +GitHash: +ModuleCount:6 +HasImagePlaneDetails:False + +Images:[module_num:1|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Add the plate images to view when testing parameters. If not, make sure it is blank prior to processing the images via the notebook.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + : + Filter images?:Images only + Select the rule criteria:and (extension does isimage) (directory doesnot containregexp ""[\\\\/]\\."") + +Metadata:[module_num:2|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['There is no need to extract metadata since we are not using the GUI to processes each plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Extract metadata?:No + Metadata data type:Text + Metadata types:{} + Extraction method count:1 + Metadata extraction method:Extract from file/folder names + Metadata source:Folder name + Regular expression to extract from file name:^(?P.*)_(?P[A-P][0-9]{2})_s(?P[0-9])_w(?P[0-9]) + Regular expression to extract from folder name:(?Plocalhost220512140003_KK22-05-198|localhost220513100001_KK22-05-198_FactinAdjusted|localhost230405150001) + Extract metadata from:All images + Select the filtering criteria:and (file does contain """") + Metadata file location:Elsewhere...| + Match file and image metadata:[] + Use case insensitive matching?:No + Metadata file name:None + Does cached metadata exist?:No + +NamesAndTypes:[module_num:3|svn_version:'Unknown'|variable_revision_number:8|show_window:False|notes:['Assign each image to its channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Assign a name to:Images matching rules + Select the image type:Grayscale image + Name to assign these images:DNA + Match metadata:[] + Image set matching method:Order + Set intensity range from:Image metadata + Assignments count:4 + Single images count:0 + Maximum intensity:255.0 + Process as 3D?:No + Relative pixel spacing in X:1.0 + Relative pixel spacing in Y:1.0 + Relative pixel spacing in Z:1.0 + Select the rule criteria:and (file does contain ""DAPI"") + Name to assign these images:OrigDAPI + Name to assign these objects:Cell + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""GFP"") + Name to assign these images:OrigGFP + Name to assign these objects:Nucleus + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""RFP"") + Name to assign these images:OrigRFP + Name to assign these objects:Cytoplasm + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""CY5"") + Name to assign these images:OrigCY5 + Name to assign these objects:Speckle + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + +Groups:[module_num:4|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since we are not using the GUI to processes each plate, we do NOT need to group the images by plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Do you want to group your images?:No + grouping metadata count:1 + Metadata category:Plate + +MeasureImageQuality:[module_num:5|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['To measure image quality, we will only be using blur and saturation to determine how out of focus images are and if there are large highly saturated artifacts. Defaults are used for blur.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Calculate metrics for which images?:All loaded images + Image count:1 + Scale count:1 + Threshold count:1 + Select the images to measure: + Include the image rescaling value?:Yes + Calculate blur metrics?:Yes + Spatial scale for blur measurements:20 + Calculate saturation metrics?:Yes + Calculate intensity metrics?:No + Calculate thresholds?:No + Use all thresholding methods?:No + Select a thresholding method:Otsu + Typical fraction of the image covered by objects:0.1 + Two-class or three-class thresholding?:Two classes + Minimize the weighted variance or the entropy?:Weighted variance + Assign pixels in the middle intensity class to the foreground or the background?:Foreground + +ExportToSpreadsheet:[module_num:6|svn_version:'Unknown'|variable_revision_number:13|show_window:False|notes:['Export all of the whole image quality metrics to a spreadsheet to load in and evaluate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the column delimiter:Comma ("","") + Add image metadata columns to your object data file?:No + Add image file and folder names to your object data file?:No + Select the measurements to export:No + Calculate the per-image mean values for object measurements?:No + Calculate the per-image median values for object measurements?:No + Calculate the per-image standard deviation values for object measurements?:No + Output file location:Default Output Folder| + Create a GenePattern GCT file?:No + Select source of sample row name:Metadata + Select the image to use as the identifier:None + Select the metadata to use as the identifier:None + Export all measurement types?:Yes + Press button to select measurements: + Representation of Nan/Inf:NaN + Add a prefix to file names?:No + Filename prefix:Whole_Image_QC + Overwrite existing files without warning?:No + Data to export:Do not use + Combine these object measurements with those of the previous object?:No + File name:DATA.csv + Use the object name for the file name?:Yes +" +Run_Timestamp,2024-01-30T15:49:14.713933 diff --git a/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3/Image.csv b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3/Image.csv new file mode 100644 index 0000000..61d0a05 --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3/Image.csv @@ -0,0 +1,1801 @@ 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diff --git a/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3_prime/Experiment.csv b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3_prime/Experiment.csv new file mode 100644 index 0000000..ea562a0 --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3_prime/Experiment.csv @@ -0,0 +1,125 @@ +Key,Value +CellProfiler_Version,4.2.4 +ChannelType_OrigCY5,Grayscale +ChannelType_OrigDAPI,Grayscale +ChannelType_OrigGFP,Grayscale +ChannelType_OrigRFP,Grayscale +ImageSet_Zip_Dictionary,b'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' +Metadata_Tags,"[""ImageNumber""]" +Pipeline_Pipeline,"CellProfiler Pipeline: http://www.cellprofiler.org +Version:5 +DateRevision:424 +GitHash: +ModuleCount:6 +HasImagePlaneDetails:False + +Images:[module_num:1|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Add the plate images to view when testing parameters. If not, make sure it is blank prior to processing the images via the notebook.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + : + Filter images?:Images only + Select the rule criteria:and (extension does isimage) (directory doesnot containregexp ""[\\\\/]\\."") + +Metadata:[module_num:2|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['There is no need to extract metadata since we are not using the GUI to processes each plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Extract metadata?:No + Metadata data type:Text + Metadata types:{} + Extraction method count:1 + Metadata extraction method:Extract from file/folder names + Metadata source:Folder name + Regular expression to extract from file name:^(?P.*)_(?P[A-P][0-9]{2})_s(?P[0-9])_w(?P[0-9]) + Regular expression to extract from folder name:(?Plocalhost220512140003_KK22-05-198|localhost220513100001_KK22-05-198_FactinAdjusted|localhost230405150001) + Extract metadata from:All images + Select the filtering criteria:and (file does contain """") + Metadata file location:Elsewhere...| + Match file and image metadata:[] + Use case insensitive matching?:No + Metadata file name:None + Does cached metadata exist?:No + +NamesAndTypes:[module_num:3|svn_version:'Unknown'|variable_revision_number:8|show_window:False|notes:['Assign each image to its channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Assign a name to:Images matching rules + Select the image type:Grayscale image + Name to assign these images:DNA + Match metadata:[] + Image set matching method:Order + Set intensity range from:Image metadata + Assignments count:4 + Single images count:0 + Maximum intensity:255.0 + Process as 3D?:No + Relative pixel spacing in X:1.0 + Relative pixel spacing in Y:1.0 + Relative pixel spacing in Z:1.0 + Select the rule criteria:and (file does contain ""DAPI"") + Name to assign these images:OrigDAPI + Name to assign these objects:Cell + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""GFP"") + Name to assign these images:OrigGFP + Name to assign these objects:Nucleus + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""RFP"") + Name to assign these images:OrigRFP + Name to assign these objects:Cytoplasm + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""CY5"") + Name to assign these images:OrigCY5 + Name to assign these objects:Speckle + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + +Groups:[module_num:4|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since we are not using the GUI to processes each plate, we do NOT need to group the images by plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Do you want to group your images?:No + grouping metadata count:1 + Metadata category:Plate + +MeasureImageQuality:[module_num:5|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['To measure image quality, we will only be using blur and saturation to determine how out of focus images are and if there are large highly saturated artifacts. Defaults are used for blur.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Calculate metrics for which images?:All loaded images + Image count:1 + Scale count:1 + Threshold count:1 + Select the images to measure: + Include the image rescaling value?:Yes + Calculate blur metrics?:Yes + Spatial scale for blur measurements:20 + Calculate saturation metrics?:Yes + Calculate intensity metrics?:No + Calculate thresholds?:No + Use all thresholding methods?:No + Select a thresholding method:Otsu + Typical fraction of the image covered by objects:0.1 + Two-class or three-class thresholding?:Two classes + Minimize the weighted variance or the entropy?:Weighted variance + Assign pixels in the middle intensity class to the foreground or the background?:Foreground + +ExportToSpreadsheet:[module_num:6|svn_version:'Unknown'|variable_revision_number:13|show_window:False|notes:['Export all of the whole image quality metrics to a spreadsheet to load in and evaluate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the column delimiter:Comma ("","") + Add image metadata columns to your object data file?:No + Add image file and folder names to your object data file?:No + Select the measurements to export:No + Calculate the per-image mean values for object measurements?:No + Calculate the per-image median values for object measurements?:No + Calculate the per-image standard deviation values for object measurements?:No + Output file location:Default Output Folder| + Create a GenePattern GCT file?:No + Select source of sample row name:Metadata + Select the image to use as the identifier:None + Select the metadata to use as the identifier:None + Export all measurement types?:Yes + Press button to select measurements: + Representation of Nan/Inf:NaN + Add a prefix to file names?:No + Filename prefix:Whole_Image_QC + Overwrite existing files without warning?:No + Data to export:Do not use + Combine these object measurements with those of the previous object?:No + File name:DATA.csv + Use the object name for the file name?:Yes +" +Run_Timestamp,2024-01-30T15:49:14.697210 diff --git a/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3_prime/Image.csv b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3_prime/Image.csv new file mode 100644 index 0000000..2251ad3 --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_3_prime/Image.csv @@ -0,0 +1,1801 @@ 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+Metadata_Tags,"[""ImageNumber""]" +Pipeline_Pipeline,"CellProfiler Pipeline: http://www.cellprofiler.org +Version:5 +DateRevision:424 +GitHash: +ModuleCount:6 +HasImagePlaneDetails:False + +Images:[module_num:1|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Add the plate images to view when testing parameters. If not, make sure it is blank prior to processing the images via the notebook.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + : + Filter images?:Images only + Select the rule criteria:and (extension does isimage) (directory doesnot containregexp ""[\\\\/]\\."") + +Metadata:[module_num:2|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['There is no need to extract metadata since we are not using the GUI to processes each plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Extract metadata?:No + Metadata data type:Text + Metadata types:{} + Extraction method count:1 + Metadata extraction method:Extract from file/folder names + Metadata source:Folder name + Regular expression to extract from file name:^(?P.*)_(?P[A-P][0-9]{2})_s(?P[0-9])_w(?P[0-9]) + Regular expression to extract from folder name:(?Plocalhost220512140003_KK22-05-198|localhost220513100001_KK22-05-198_FactinAdjusted|localhost230405150001) + Extract metadata from:All images + Select the filtering criteria:and (file does contain """") + Metadata file location:Elsewhere...| + Match file and image metadata:[] + Use case insensitive matching?:No + Metadata file name:None + Does cached metadata exist?:No + +NamesAndTypes:[module_num:3|svn_version:'Unknown'|variable_revision_number:8|show_window:False|notes:['Assign each image to its channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Assign a name to:Images matching rules + Select the image type:Grayscale image + Name to assign these images:DNA + Match metadata:[] + Image set matching method:Order + Set intensity range from:Image metadata + Assignments count:4 + Single images count:0 + Maximum intensity:255.0 + Process as 3D?:No + Relative pixel spacing in X:1.0 + Relative pixel spacing in Y:1.0 + Relative pixel spacing in Z:1.0 + Select the rule criteria:and (file does contain ""DAPI"") + Name to assign these images:OrigDAPI + Name to assign these objects:Cell + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""GFP"") + Name to assign these images:OrigGFP + Name to assign these objects:Nucleus + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""RFP"") + Name to assign these images:OrigRFP + Name to assign these objects:Cytoplasm + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""CY5"") + Name to assign these images:OrigCY5 + Name to assign these objects:Speckle + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + +Groups:[module_num:4|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since we are not using the GUI to processes each plate, we do NOT need to group the images by plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Do you want to group your images?:No + grouping metadata count:1 + Metadata category:Plate + +MeasureImageQuality:[module_num:5|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['To measure image quality, we will only be using blur and saturation to determine how out of focus images are and if there are large highly saturated artifacts. Defaults are used for blur.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Calculate metrics for which images?:All loaded images + Image count:1 + Scale count:1 + Threshold count:1 + Select the images to measure: + Include the image rescaling value?:Yes + Calculate blur metrics?:Yes + Spatial scale for blur measurements:20 + Calculate saturation metrics?:Yes + Calculate intensity metrics?:No + Calculate thresholds?:No + Use all thresholding methods?:No + Select a thresholding method:Otsu + Typical fraction of the image covered by objects:0.1 + Two-class or three-class thresholding?:Two classes + Minimize the weighted variance or the entropy?:Weighted variance + Assign pixels in the middle intensity class to the foreground or the background?:Foreground + +ExportToSpreadsheet:[module_num:6|svn_version:'Unknown'|variable_revision_number:13|show_window:False|notes:['Export all of the whole image quality metrics to a spreadsheet to load in and evaluate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the column delimiter:Comma ("","") + Add image metadata columns to your object data file?:No + Add image file and folder names to your object data file?:No + Select the measurements to export:No + Calculate the per-image mean values for object measurements?:No + Calculate the per-image median values for object measurements?:No + Calculate the per-image standard deviation values for object measurements?:No + Output file location:Default Output Folder| + Create a GenePattern GCT file?:No + Select source of sample row name:Metadata + Select the image to use as the identifier:None + Select the metadata to use as the identifier:None + Export all measurement types?:Yes + Press button to select measurements: + Representation of Nan/Inf:NaN + Add a prefix to file names?:No + Filename prefix:Whole_Image_QC + Overwrite existing files without warning?:No + Data to export:Do not use + Combine these object measurements with those of the previous object?:No + File name:DATA.csv + Use the object name for the file name?:Yes +" +Run_Timestamp,2024-01-30T15:49:14.654308 diff --git a/1.cellprofiler_ic/image_quality_control/qc_results/Plate_4/Image.csv b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_4/Image.csv new file mode 100644 index 0000000..02f0fe0 --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_4/Image.csv @@ -0,0 +1,1501 @@ 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diff --git a/1.cellprofiler_ic/image_quality_control/qc_results/Plate_5/Experiment.csv b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_5/Experiment.csv new file mode 100644 index 0000000..28380d3 --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_5/Experiment.csv @@ -0,0 +1,125 @@ +Key,Value +CellProfiler_Version,4.2.4 +ChannelType_OrigCY5,Grayscale +ChannelType_OrigDAPI,Grayscale +ChannelType_OrigGFP,Grayscale +ChannelType_OrigRFP,Grayscale 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+Metadata_Tags,"[""ImageNumber""]" +Pipeline_Pipeline,"CellProfiler Pipeline: http://www.cellprofiler.org +Version:5 +DateRevision:424 +GitHash: +ModuleCount:6 +HasImagePlaneDetails:False + +Images:[module_num:1|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Add the plate images to view when testing parameters. If not, make sure it is blank prior to processing the images via the notebook.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + : + Filter images?:Images only + Select the rule criteria:and (extension does isimage) (directory doesnot containregexp ""[\\\\/]\\."") + +Metadata:[module_num:2|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['There is no need to extract metadata since we are not using the GUI to processes each plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Extract metadata?:No + Metadata data type:Text + Metadata types:{} + Extraction method count:1 + Metadata extraction method:Extract from file/folder names + Metadata source:Folder name + Regular expression to extract from file name:^(?P.*)_(?P[A-P][0-9]{2})_s(?P[0-9])_w(?P[0-9]) + Regular expression to extract from folder name:(?Plocalhost220512140003_KK22-05-198|localhost220513100001_KK22-05-198_FactinAdjusted|localhost230405150001) + Extract metadata from:All images + Select the filtering criteria:and (file does contain """") + Metadata file location:Elsewhere...| + Match file and image metadata:[] + Use case insensitive matching?:No + Metadata file name:None + Does cached metadata exist?:No + +NamesAndTypes:[module_num:3|svn_version:'Unknown'|variable_revision_number:8|show_window:False|notes:['Assign each image to its channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Assign a name to:Images matching rules + Select the image type:Grayscale image + Name to assign these images:DNA + Match metadata:[] + Image set matching method:Order + Set intensity range from:Image metadata + Assignments count:4 + Single images count:0 + Maximum intensity:255.0 + Process as 3D?:No + Relative pixel spacing in X:1.0 + Relative pixel spacing in Y:1.0 + Relative pixel spacing in Z:1.0 + Select the rule criteria:and (file does contain ""DAPI"") + Name to assign these images:OrigDAPI + Name to assign these objects:Cell + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""GFP"") + Name to assign these images:OrigGFP + Name to assign these objects:Nucleus + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""RFP"") + Name to assign these images:OrigRFP + Name to assign these objects:Cytoplasm + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain ""CY5"") + Name to assign these images:OrigCY5 + Name to assign these objects:Speckle + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + +Groups:[module_num:4|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since we are not using the GUI to processes each plate, we do NOT need to group the images by plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Do you want to group your images?:No + grouping metadata count:1 + Metadata category:Plate + +MeasureImageQuality:[module_num:5|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['To measure image quality, we will only be using blur and saturation to determine how out of focus images are and if there are large highly saturated artifacts. Defaults are used for blur.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Calculate metrics for which images?:All loaded images + Image count:1 + Scale count:1 + Threshold count:1 + Select the images to measure: + Include the image rescaling value?:Yes + Calculate blur metrics?:Yes + Spatial scale for blur measurements:20 + Calculate saturation metrics?:Yes + Calculate intensity metrics?:No + Calculate thresholds?:No + Use all thresholding methods?:No + Select a thresholding method:Otsu + Typical fraction of the image covered by objects:0.1 + Two-class or three-class thresholding?:Two classes + Minimize the weighted variance or the entropy?:Weighted variance + Assign pixels in the middle intensity class to the foreground or the background?:Foreground + +ExportToSpreadsheet:[module_num:6|svn_version:'Unknown'|variable_revision_number:13|show_window:False|notes:['Export all of the whole image quality metrics to a spreadsheet to load in and evaluate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the column delimiter:Comma ("","") + Add image metadata columns to your object data file?:No + Add image file and folder names to your object data file?:No + Select the measurements to export:No + Calculate the per-image mean values for object measurements?:No + Calculate the per-image median values for object measurements?:No + Calculate the per-image standard deviation values for object measurements?:No + Output file location:Default Output Folder| + Create a GenePattern GCT file?:No + Select source of sample row name:Metadata + Select the image to use as the identifier:None + Select the metadata to use as the identifier:None + Export all measurement types?:Yes + Press button to select measurements: + Representation of Nan/Inf:NaN + Add a prefix to file names?:No + Filename prefix:Whole_Image_QC + Overwrite existing files without warning?:No + Data to export:Do not use + Combine these object measurements with those of the previous object?:No + File name:DATA.csv + Use the object name for the file name?:Yes +" +Run_Timestamp,2024-01-30T15:49:14.723586 diff --git a/1.cellprofiler_ic/image_quality_control/qc_results/Plate_5/Image.csv b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_5/Image.csv new file mode 100644 index 0000000..9de564c --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/qc_results/Plate_5/Image.csv @@ -0,0 +1,1513 @@ 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diff --git a/1.cellprofiler_ic/image_quality_control/scripts/0.whole_image_qc.py b/1.cellprofiler_ic/image_quality_control/scripts/0.whole_image_qc.py new file mode 100644 index 0000000..8a16349 --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/scripts/0.whole_image_qc.py @@ -0,0 +1,82 @@ +#!/usr/bin/env python +# coding: utf-8 + +# # Run whole image QC pipeline in CellProfiler +# + +# ## Import libraries + +# In[1]: + + +import pathlib +import pprint + +import sys + +sys.path.append("../../utils") +import cp_parallel + + +# ## Set paths and variables + +# In[2]: + + +# set the run type for the parallelization +run_name = "quality_control" + +# set path for pipeline for illumination correction +path_to_pipeline = pathlib.Path("../pipelines/whole_image_qc.cppipe").resolve(strict=True) + +# set main output dir for all plates if it doesn't exist +output_dir = pathlib.Path("./qc_results") +output_dir.mkdir(exist_ok=True) + +# directory where images are located within folders +images_dir = pathlib.Path("../../0.download_data") + +# list for plate names based on folders to use to create dictionary +plate_names = [] +# iterate through 0.download_data and append plate names from folder names that contain image data from that plate +for file_path in images_dir.iterdir(): + if str(file_path.stem).startswith("Plate"): + plate_names.append(str(file_path.stem)) + +print(plate_names) +print("There are a total of", len(plate_names), "plates. The names of the plates are:") +for plate in plate_names: + print(plate) + + +# ## Generate dictionary with plate info to run CellProfiler + +# In[3]: + + +# create plate info dictionary with all parts of the CellProfiler CLI command to run in parallel +plate_info_dictionary = { + name: { + "path_to_images": pathlib.Path(list(images_dir.rglob(name))[0]).resolve( + strict=True + ), + "path_to_output": pathlib.Path(f"{output_dir}/{name}"), + "path_to_pipeline": path_to_pipeline, + + } + for name in plate_names if not name=='Plate_1' and not name=='Plate_2' # Do not include pilot datasets +} + +# view the dictionary to assess that all info is added correctly +pprint.pprint(plate_info_dictionary, indent=4) + + +# ## Run QC pipeline in CellProfiler + +# In[ ]: + + +cp_parallel.run_cellprofiler_parallel( + plate_info_dictionary=plate_info_dictionary, run_name=run_name +) + diff --git a/1.cellprofiler_ic/image_quality_control/scripts/1.evaluate_qc.py b/1.cellprofiler_ic/image_quality_control/scripts/1.evaluate_qc.py new file mode 100644 index 0000000..4842b5b --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/scripts/1.evaluate_qc.py @@ -0,0 +1,391 @@ +#!/usr/bin/env python +# coding: utf-8 + +# # Whole image quality control metric evaluation +# +# In this notebook, we will use the outputted QC metrics to start working on developing thresholds using z-score to flag and skip images during CellProfiler illumination correction. We are evaluating plates 3-5 to find generalizable metrics and thresholds for this dataset. +# +# **Blur metric to detect out of focus images** -> PowerLogLogSlope +# +# **Saturation metric to detect large smudges** -> PercentMaximal + +# ## Import libraries + +# In[1]: + + +import pathlib +import pandas as pd +import numpy as np + +from scipy.stats import zscore +import matplotlib.pyplot as plt +import seaborn as sns + + +# ## Set paths and load in data frame + +# In[2]: + + +# Directory for figures to be outputted +figure_dir = pathlib.Path("./qc_figures") +figure_dir.mkdir(exist_ok=True) + +# Directory with QC CellProfiler output per plate +qc_dir = pathlib.Path("./qc_results") + +# Create an empty dictionary to store data frames for each plate +all_qc_data_frames = {} + +# List all plates +plates = ["Plate_3", "Plate_3_prime", "Plate_4", "Plate_5"] + +# Loop through each plate +for plate in plates: + # Read in CSV with all image quality metrics per image for the current plate + qc_df = pd.read_csv(pathlib.Path(qc_dir / plate / "Image.csv")) + + # Adding 'Metadata_Well' and 'Metadata_Site' columns using filename from FileName_OrigActin (same for all channels) + qc_df["Metadata_Well"] = qc_df["FileName_OrigRFP"].str.split("_").str[0] + qc_df["Metadata_Site"] = qc_df["FileName_OrigRFP"].str.split("_").str[3] + + # Create a global variable with each plate as a data frame + globals()[f"{plate}_df"] = qc_df + + # Store the data frame for the current plate in the dictionary + all_qc_data_frames[plate] = qc_df + +# view the dictionary to assess that all info is added correctly +print(all_qc_data_frames.keys()) + +# assess that the data frames per plate have correct number of image sets +print(Plate_5_df.shape) +Plate_5_df.head() + + +# ## Create concat data frames combining blur and saturation metrics from all channels for all plates + +# In[3]: + + +# List of channels +channels = ["DAPI", "GFP", "RFP", "CY5"] + +# Create an empty dictionary to store data frames for each channel +all_combined_dfs = {} + +# Iterate through each channel +for channel in channels: + # Create an empty list to store data frames for each plate + plate_dfs = [] + + # Iterate through each plate and create the specified data frame for the channel + for plate, qc_df in all_qc_data_frames.items(): + plate_df = qc_df.filter(like="Metadata_").copy() + + # Add PowerLogLogSlope column + plate_df[f"ImageQuality_PowerLogLogSlope"] = qc_df[ + f"ImageQuality_PowerLogLogSlope_Orig{channel}" + ] + + # Add PercentMaximal column + plate_df[f"ImageQuality_PercentMaximal"] = qc_df[ + f"ImageQuality_PercentMaximal_Orig{channel}" + ] + + # Add "Channel" column + plate_df["Channel"] = channel + + # Add "Metadata_Plate" column + plate_df["Metadata_Plate"] = plate + + # Append the data frame to the list + plate_dfs.append(plate_df) + + # Concatenate data frames for each plate for the current channel + all_combined_dfs[channel] = pd.concat( + plate_dfs, keys=list(all_qc_data_frames.keys()), names=["metadata_plate", None] + ) + +# Concatenate the channel data frames together for plotting +df = pd.concat(list(all_combined_dfs.values()), ignore_index=True) + +print(df.shape) +df.head() + + +# ## Blur metric +# +# Based on the plots below, we can see that blur is a big impact on the dataset. Traditionally, we expect values close to 0 are poor quality, but we can see in these plates that values that are very negative and close to 0 are both poor quality images. +# +# We are seeing that close to 0 images are empty and images that are very negative are very out-of-focus. +# +# We use the z-score method to both find threshold values for identifying poor quality images above and below the mean and to output a data frame with outliers to view these images in a separate software (e.g., ImageJ/FIJI). We use **2 standard deviations** away from the mean for assessing outliers. + +# In[4]: + + +summary_statistics = df["ImageQuality_PowerLogLogSlope"].describe() +print(summary_statistics) + + +# ### Use z-score to find outliers 2 standard deviations above and below the mean to view as a data frame + +# In[5]: + + +# Calculate Z-scores for the column with all plates +z_scores = zscore(df["ImageQuality_PowerLogLogSlope"]) + +# Set a threshold for Z-scores (adjust as needed for number of standard deviations away from the mean) +threshold_z = 2 + +# Identify outlier rows based on Z-scores above and below the mean since we are using absolute values of the z-scores +blur_outliers = df[abs(z_scores) > threshold_z] + +# Identify unique well + site combos among outliers +unique_combos = blur_outliers[["Metadata_Well", "Metadata_Site"]].drop_duplicates() + +print(blur_outliers["Channel"].value_counts()) +# Print example outliers to visualize +blur_outliers.sort_values(by="ImageQuality_PowerLogLogSlope").head() + + +# ### Identify threshold values to use to find outliers above and below the mean +# +# **Note:** These values will be used in CellProfiler to flag and not process images. We will round the values to the second decimal to use in the pipeline. + +# In[6]: + + +# Calculate the mean and standard deviation +mean_value = df["ImageQuality_PowerLogLogSlope"].mean() +std_dev = df["ImageQuality_PowerLogLogSlope"].std() + +# Set the threshold multiplier for above and below the mean +threshold = 2 + +# Calculate the threshold values +threshold_value_above_mean = mean_value + threshold * std_dev +threshold_value_below_mean = mean_value - threshold * std_dev + +# Print the calculated threshold values +print("Threshold for outliers above the mean:", threshold_value_above_mean) +print("Threshold for outliers below the mean:", threshold_value_below_mean) + + +# **We will be using these thresholds in the CellProfiler pipeline. We will set anything `greater` than -1.37 as outliers and anything `below` -2.50 as outliers.** + +# ### Generate individual density plots per plate and save + +# In[7]: + + +# Set the style +sns.set_style("whitegrid") + +# Iterate through each plate +for plate in df["Metadata_Plate"].unique(): + plate_df = df[df["Metadata_Plate"] == plate] + + sns.kdeplot( + data=plate_df, + x="ImageQuality_PowerLogLogSlope", + hue="Channel", + palette=["b", "g", "r", "magenta"], + fill=True, + common_norm=False, + ) + + plt.title(f"Density plots per channel for {plate}") + plt.xlabel("ImageQuality_PowerLogLogSlope") + plt.ylabel("Density") + + plt.tight_layout() + plt.savefig( + pathlib.Path(f"{figure_dir}/{plate}_channels_blur_density.png"), dpi=500 + ) + plt.close() # Close the plot to prevent it from being displayed + + +# ### Generate density plot with all plates together and save + +# In[8]: + + +# Number of plates +num_plates = len(df["Metadata_Plate"].unique()) + +# Calculate the number of rows and columns for the subplots +num_rows = 2 # You want 2 rows for the quad order +num_cols = (num_plates + 1) // num_rows # +1 to round up + +# Set the style +sns.set_style("whitegrid") + +# Create subplots with custom layout +fig, axes = plt.subplots(nrows=num_rows, ncols=num_cols, figsize=(15, 5 * num_rows)) + +# Flatten the axes array for easier indexing +axes = axes.flatten() + +# Iterate through each plate +for idx, plate in enumerate(df["Metadata_Plate"].unique()): + # Plot all channels in the same subplot + ax = axes[idx] + plate_df = df[df["Metadata_Plate"] == plate] + sns.kdeplot( + data=plate_df, + x="ImageQuality_PowerLogLogSlope", + hue="Channel", + palette=["b", "g", "r", "magenta"], + fill=True, + common_norm=False, + ax=ax, + ) + + # Set vertical lines at -2.5 and -1.25 + ax.axvline(x=threshold_value_above_mean, color="red", linestyle="--") + ax.axvline(x=threshold_value_below_mean, color="red", linestyle="--") + + ax.set_title(f"Density plots for {plate}") + ax.set_xlabel("ImageQuality_PowerLogLogSlope") + ax.set_ylabel("Density") + + # Set the x-axis range for the current subplot + ax.set_xlim(-4.0, 0) + +# Remove empty subplots +for i in range(num_plates, len(axes)): + fig.delaxes(axes[i]) + +# Adjust layout +plt.tight_layout() + +# Save the plot +plt.savefig(figure_dir / "all_channels_combined_density.png", dpi=500) + +plt.close() # Close the plot to prevent it from being displayed + + +# ### Visualize the distribution of the identified outliers + +# In[9]: + + +# Plot outliers as a distribution plot with hue as 'Metadata_Plate' +sns.set_style("whitegrid") +plt.figure(figsize=(12, 8)) + +# Using kdeplot (you can also use distplot) +sns.kdeplot( + data=blur_outliers, + x="ImageQuality_PowerLogLogSlope", + hue="Metadata_Plate", + palette="viridis", + fill=True, + common_norm=False, +) + +# Set vertical lines at -2.5 and 1.25 +plt.axvline(x=threshold_value_above_mean, color="red", linestyle="--") +plt.axvline(x=threshold_value_below_mean, color="red", linestyle="--") + +plt.title("Distribution of Outliers per Plate") +plt.xlabel("ImageQuality_PowerLogLogSlope") + +plt.tight_layout() + +plt.savefig(figure_dir / "blur_outliers_per_plate.png", dpi=500) + +plt.show() + + +# ## Saturation metric +# +# For saturation metrics, we are looking for: +# +# - Smudged images or images containing large artifacts (usually seen in `DNA` channel regardless of stain) +# - Overly saturated channels which can occur in any channel, but specifically we are seeing `Mito` having the most problems with this. +# +# This means that we will only be setting threshold for outliers for all channels just like for blur. + +# In[10]: + + +summary_statistics = df["ImageQuality_PercentMaximal"].describe() +print(summary_statistics) + + +# ### Visualize density plot of saturation metric + +# In[11]: + + +# Create a KDE plot with separate lines for each Metadata_Plate +plt.figure(figsize=(10, 6)) +sns.kdeplot( + x=np.log1p(df["ImageQuality_PercentMaximal"]), hue=df["Metadata_Plate"], fill=True +) + +# Add a vertical line at the log-transformed value of 1.4 +plt.axvline(x=np.log1p(1.4), color='red', linestyle='--', label='Log-transformed 1.4') + +# Set labels and title +plt.ylabel("Density") +plt.xlabel("Log-transformed Percent Maximal") +plt.title("KDE Plot of Log-transformed Percent Maximal by Plate") + +plt.savefig(figure_dir / "saturation_outliers_per_plate.png", dpi=500) + +# Show the plot +plt.show() + + +# ### Use z-score to find outliers 2 standard deviations above the mean to view as a data frame + +# In[12]: + + +# Calculate Z-scores for the column +z_scores = zscore(df["ImageQuality_PercentMaximal"]) + +# Set a threshold for Z-scores (adjust as needed for number of standard deviations away from the mean) +threshold_z = 2 + +# Identify outlier rows based on Z-scores greater than as to identify whole images with abnormally high saturated pixels +saturation_outliers = df[abs(z_scores) > threshold_z] + +# Identify unique well + site combos among outliers +unique_combos = saturation_outliers[ + ["Metadata_Well", "Metadata_Site"] +].drop_duplicates() + +# Visualize example images through FIJI using data frame identifying outliers +print(saturation_outliers["Channel"].value_counts()) +saturation_outliers.sort_values(by="ImageQuality_PercentMaximal", ascending=True).head() + + +# ### Identify threshold values to use to find outliers above the mean +# +# **Note:** This value will be used in CellProfiler to flag and not process images. We will round to the second decimal to use in the pipeline. + +# In[13]: + + +# Calculate the mean and standard deviation +mean_value = df["ImageQuality_PercentMaximal"].mean() +std_dev = df["ImageQuality_PercentMaximal"].std() + +# Set the threshold multiplier for above the mean +threshold = 2 + +# Calculate the threshold values +threshold_value_above_mean = mean_value + threshold * std_dev + +# Print the calculated threshold values +print("Threshold for outliers above the mean:", threshold_value_above_mean) + + +# **We will be using 1.40 as the threshold for anything that `above` this is considered a outlier for all channels.** diff --git a/1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.py b/1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.py new file mode 100644 index 0000000..bf84471 --- /dev/null +++ b/1.cellprofiler_ic/image_quality_control/scripts/2.qc_report.py @@ -0,0 +1,207 @@ +#!/usr/bin/env python +# coding: utf-8 + +# # Whole Image Quality Control Report + +# ## Import libraries + +# In[1]: + + +suppressPackageStartupMessages(library(dplyr)) +suppressPackageStartupMessages(library(ggplot2)) +suppressPackageStartupMessages(library(platetools)) +suppressPackageStartupMessages(library(stringr)) +suppressPackageStartupMessages(library(arrow)) +suppressPackageStartupMessages(library(RColorBrewer)) + + +# ## Set paths and variables + +# In[2]: + + +# Paths to CSV files to generate QC report +path_to_qc_results <- file.path("../Corrected_Images/Corrected_Plate_5/IC_QC_RunImage.csv") + +# Output path for bar chart +barchart_output_file <- file.path(paste0("./qc_figures/Plate_5_flagged_fov_per_well_chart.png")) + +# Output path for platemap +platemap_output_file <- file.path(paste0("./qc_figures/Plate_5_platemap_flagged_fov_per_well.png")) + +# Output path for site bar plot +site_FOV_output_file <- file.path(paste0("./qc_figures/Plate_5_per_site_flagged_fov.png")) + + +# ## Load in outlier and quality control results + +# In[3]: + + +# Read in CSV files +qc_df <- read.csv(path_to_qc_results) + +dim(qc_df) +head(qc_df) + + +# ## Generate data frame removing poor quality sites and counting the number of sites left per well + +# In[4]: + + +# Count the number of flagged image sets +flagged_image_sets <- sum(qc_df$Image_Quality_Control_QC_Flag == 1) + +well_flag_counts <- qc_df %>% + group_by(Metadata_Well) %>% + summarise(FlaggedFOVs = sum(Image_Quality_Control_QC_Flag)) + +# Print the number of image sets flagged and not processed +print(flagged_image_sets) +# Print the table +head(well_flag_counts) + + +# ## Generate bar chart to show how many FOVs were flagged after QC + +# In[5]: + + +# Extract the first letter from 'Metadata_Well' to create a color palette +unique_starting_letters <- unique(substr(well_flag_counts$Metadata_Well, 1, 1)) + +# Create a color palette based on unique starting letters +color_palette <- colorRampPalette(brewer.pal(8, "Set1"))(length(unique_starting_letters)) + +# Map each unique starting letter to a color in a named vector +color_dict <- setNames(color_palette, unique_starting_letters) + +# Create a new column 'Color' in the data frame based on the starting letter +well_flag_counts$Color <- color_dict[substr(well_flag_counts$Metadata_Well, 1, 1)] + +# Increase the figure size to extend the chart horizontally +options(repr.plot.width=14, repr.plot.height=6) + +# Create a bar chart using ggplot2 with the 'Color' column for colors +fov_chart <- ggplot(well_flag_counts, aes(x = Metadata_Well, y = FlaggedFOVs, fill = Color)) + + geom_bar(stat = 'identity') + + labs(x = 'Metadata_Well', y = 'Number of Flagged FOVs', title = 'Count of Flagged FOVs per Well for Plate 5') + + scale_fill_identity() + + theme_bw() + + theme(axis.text.x = element_text(angle = 45, hjust = 1)) + +# Save plot to qc_figures +ggsave( + barchart_output_file, + fov_chart, + dpi = 500, + height = 6, + width = 10 + ) + +# Display the plot in the notebook +print(fov_chart) + + +# ## Generate plate map to show distribution of flagged FOVs from QC + +# In[6]: + + +# Add genotype to data frame to label on plot +well_flag_counts <- well_flag_counts %>% + mutate( + Metadata_genotype = case_when( + as.numeric(gsub("\\D", "", Metadata_Well)) %in% 1:4 ~ "WT", + as.numeric(gsub("\\D", "", Metadata_Well)) %in% 5:8 ~ "HET", + as.numeric(gsub("\\D", "", Metadata_Well)) %in% 9:12 ~ "Null", + ) + ) + +fov_platemap <- platetools::raw_map( + data = well_flag_counts$FlaggedFOVs, + well = well_flag_counts$Metadata_Well, + plate = 96, + size = 8 + ) + + + ggtitle(paste("Platemap of Flagged FOV Count Per Well in Plate 5")) + + geom_point(aes(shape = well_flag_counts$Metadata_genotype)) + + scale_shape_discrete(name = "Genotype") + + scale_fill_gradient( + name = "Flagged FOV Count", + low = "white", + high = "red", + ) + + theme( + plot.title = element_text(size = 10, face = "bold"), + legend.text = element_text(size = 8), # Adjust the text size + legend.title = element_text(size = 8), # Adjust the title size + legend.key.size = unit(0.5, "cm") # Adjust the key size + ) + + ggsave( + platemap_output_file, + fov_platemap, + dpi = 500, + height = 3.5, + width = 6 + ) + +# Display the plot in the notebook +print(fov_platemap) + + +# ## Count of flagged FOVs based on site number + +# In[7]: + + +# Replace 'path_to_qc_results' with your actual data frame name +flagged_qc_df <- qc_df[qc_df$Image_Quality_Control_QC_Flag == 1, ] + +dim(flagged_qc_df) + +# Example using viridis palette +site_FOV_plot <- ggplot(flagged_qc_df, aes(x = factor(Metadata_Site))) + + geom_bar(fill = "steelblue") + + labs(title = "Count Plot of Site Integers", + x = "Site", + y = "Count") + +# Save plot to qc_figures +ggsave( + site_FOV_output_file, + site_FOV_plot, + dpi = 500, + height = 6, + width = 10 + ) + + +# ## Determine how many misclassified cells match with flagged images from QC (from training ML without QC) + +# In[8]: + + +# Load the parquet file into misclassed_df +misclassed_df <- arrow::read_parquet("./misclassified_cells.parquet") + +dim(misclassed_df) + +# Convert Metadata_Site to character in flagged_qc_df +flagged_qc_df <- flagged_qc_df %>% + mutate(Metadata_Site = as.character(Metadata_Site)) + +# Convert Metadata_Site to character in misclassed_df +misclassed_df <- misclassed_df %>% + mutate(Metadata_Site = as.character(Metadata_Site)) + +# Perform anti-join +filtered_misclassed_df <- misclassed_df %>% + anti_join(flagged_qc_df, by = c("Metadata_Well", "Metadata_Site")) + +dim(filtered_misclassed_df) + diff --git a/1.cellprofiler_ic/nf1_ic.ipynb b/1.cellprofiler_ic/nf1_ic.ipynb index 45a9caa..ad94aab 100644 --- a/1.cellprofiler_ic/nf1_ic.ipynb +++ b/1.cellprofiler_ic/nf1_ic.ipynb @@ -56,8 +56,8 @@ "# set the run type for the parallelization\n", "run_name = \"illum_correction\"\n", "\n", - "# set path for pipeline for all plates for whole image analysis\n", - "path_to_pipeline = pathlib.Path(\"./pipelines/nuclei_analysis.cppipe\").resolve()\n", + "# Directory with pipelines\n", + "pipeline_dir = pathlib.Path(\"./pipelines/\").resolve(strict=True)\n", "\n", "# set main output dir for all plates\n", "output_dir = pathlib.Path(\"./Corrected_Images\")\n", @@ -94,7 +94,7 @@ "text": [ "{ 'Plate_5': { 'path_to_images': PosixPath('/home/jenna/nf1_cellpainting_data/0.download_data/Plate_5'),\n", " 'path_to_output': PosixPath('Corrected_Images/Corrected_Plate_5'),\n", - " 'path_to_pipeline': PosixPath('/home/jenna/nf1_cellpainting_data/1.cellprofiler_ic/NF1_illum_4channel.cppipe')}}\n" + " 'path_to_pipeline': PosixPath('/home/jenna/nf1_cellpainting_data/1.cellprofiler_ic/pipelines/NF1_illum_4channel.cppipe')}}\n" ] } ], @@ -114,12 +114,12 @@ "for name, info in plate_info_dictionary.items():\n", " # only plates 1 and 2 have 3 channels so these are the only plates that use this path\n", " if name == \"Plate_1\" or name == \"Plate_2\":\n", - " info[\"path_to_pipeline\"] = pathlib.Path(f\"./NF1_illum_3channel.cppipe\").resolve(\n", + " info[\"path_to_pipeline\"] = pathlib.Path(f\"{pipeline_dir}/NF1_illum_3channel.cppipe\").resolve(\n", " strict=True\n", " )\n", " # all other plates have 4 channels and will use that specific pipeline\n", " else:\n", - " info[\"path_to_pipeline\"] = pathlib.Path(f\"./NF1_illum_4channel.cppipe\").resolve(\n", + " info[\"path_to_pipeline\"] = pathlib.Path(f\"{pipeline_dir}/NF1_illum_4channel.cppipe\").resolve(\n", " strict=True\n", " )\n", "\n", @@ -139,25 +139,9 @@ }, { "cell_type": "code", - "execution_count": 4, + "execution_count": null, "metadata": {}, - "outputs": [ - { - "ename": "KeyboardInterrupt", - "evalue": "", - "output_type": "error", - "traceback": [ - "\u001b[0;31m---------------------------------------------------------------------------\u001b[0m", - "\u001b[0;31mKeyboardInterrupt\u001b[0m Traceback (most recent call last)", - "Cell \u001b[0;32mIn[4], line 1\u001b[0m\n\u001b[0;32m----> 1\u001b[0m cp_parallel\u001b[39m.\u001b[39;49mrun_cellprofiler_parallel(\n\u001b[1;32m 2\u001b[0m plate_info_dictionary\u001b[39m=\u001b[39;49mplate_info_dictionary, run_name\u001b[39m=\u001b[39;49mrun_name\n\u001b[1;32m 3\u001b[0m )\n", - "File \u001b[0;32m~/nf1_cellpainting_data/1.cellprofiler_ic/../utils/cp_parallel.py:119\u001b[0m, in \u001b[0;36mrun_cellprofiler_parallel\u001b[0;34m(plate_info_dictionary, run_name)\u001b[0m\n\u001b[1;32m 109\u001b[0m futures: List[Future] \u001b[39m=\u001b[39m [\n\u001b[1;32m 110\u001b[0m executor\u001b[39m.\u001b[39msubmit(\n\u001b[1;32m 111\u001b[0m subprocess\u001b[39m.\u001b[39mrun,\n\u001b[0;32m (...)\u001b[0m\n\u001b[1;32m 115\u001b[0m \u001b[39mfor\u001b[39;00m command \u001b[39min\u001b[39;00m commands\n\u001b[1;32m 116\u001b[0m ]\n\u001b[1;32m 118\u001b[0m \u001b[39m# the list of CompletedProcesses holds all the information from the CellProfiler run\u001b[39;00m\n\u001b[0;32m--> 119\u001b[0m results: List[subprocess\u001b[39m.\u001b[39mCompletedProcess] \u001b[39m=\u001b[39m [future\u001b[39m.\u001b[39mresult() \u001b[39mfor\u001b[39;00m future \u001b[39min\u001b[39;00m futures]\n\u001b[1;32m 121\u001b[0m \u001b[39mprint\u001b[39m(\u001b[39m\"\u001b[39m\u001b[39mAll processes have been completed!\u001b[39m\u001b[39m\"\u001b[39m)\n\u001b[1;32m 123\u001b[0m \u001b[39m# for each process, confirm that the process completed succesfully and return a log file\u001b[39;00m\n", - "File \u001b[0;32m~/nf1_cellpainting_data/1.cellprofiler_ic/../utils/cp_parallel.py:119\u001b[0m, in \u001b[0;36m\u001b[0;34m(.0)\u001b[0m\n\u001b[1;32m 109\u001b[0m futures: List[Future] \u001b[39m=\u001b[39m [\n\u001b[1;32m 110\u001b[0m executor\u001b[39m.\u001b[39msubmit(\n\u001b[1;32m 111\u001b[0m subprocess\u001b[39m.\u001b[39mrun,\n\u001b[0;32m (...)\u001b[0m\n\u001b[1;32m 115\u001b[0m \u001b[39mfor\u001b[39;00m command \u001b[39min\u001b[39;00m commands\n\u001b[1;32m 116\u001b[0m ]\n\u001b[1;32m 118\u001b[0m \u001b[39m# the list of CompletedProcesses holds all the information from the CellProfiler run\u001b[39;00m\n\u001b[0;32m--> 119\u001b[0m results: List[subprocess\u001b[39m.\u001b[39mCompletedProcess] \u001b[39m=\u001b[39m [future\u001b[39m.\u001b[39;49mresult() \u001b[39mfor\u001b[39;00m future \u001b[39min\u001b[39;00m futures]\n\u001b[1;32m 121\u001b[0m \u001b[39mprint\u001b[39m(\u001b[39m\"\u001b[39m\u001b[39mAll processes have been completed!\u001b[39m\u001b[39m\"\u001b[39m)\n\u001b[1;32m 123\u001b[0m \u001b[39m# for each process, confirm that the process completed succesfully and return a log file\u001b[39;00m\n", - "File \u001b[0;32m~/anaconda3/envs/nf1_cellpainting_data/lib/python3.8/concurrent/futures/_base.py:439\u001b[0m, in \u001b[0;36mFuture.result\u001b[0;34m(self, timeout)\u001b[0m\n\u001b[1;32m 436\u001b[0m \u001b[39melif\u001b[39;00m \u001b[39mself\u001b[39m\u001b[39m.\u001b[39m_state \u001b[39m==\u001b[39m FINISHED:\n\u001b[1;32m 437\u001b[0m \u001b[39mreturn\u001b[39;00m \u001b[39mself\u001b[39m\u001b[39m.\u001b[39m__get_result()\n\u001b[0;32m--> 439\u001b[0m \u001b[39mself\u001b[39;49m\u001b[39m.\u001b[39;49m_condition\u001b[39m.\u001b[39;49mwait(timeout)\n\u001b[1;32m 441\u001b[0m \u001b[39mif\u001b[39;00m \u001b[39mself\u001b[39m\u001b[39m.\u001b[39m_state \u001b[39min\u001b[39;00m [CANCELLED, CANCELLED_AND_NOTIFIED]:\n\u001b[1;32m 442\u001b[0m \u001b[39mraise\u001b[39;00m CancelledError()\n", - "File \u001b[0;32m~/anaconda3/envs/nf1_cellpainting_data/lib/python3.8/threading.py:302\u001b[0m, in \u001b[0;36mCondition.wait\u001b[0;34m(self, timeout)\u001b[0m\n\u001b[1;32m 300\u001b[0m \u001b[39mtry\u001b[39;00m: \u001b[39m# restore state no matter what (e.g., KeyboardInterrupt)\u001b[39;00m\n\u001b[1;32m 301\u001b[0m \u001b[39mif\u001b[39;00m timeout \u001b[39mis\u001b[39;00m \u001b[39mNone\u001b[39;00m:\n\u001b[0;32m--> 302\u001b[0m waiter\u001b[39m.\u001b[39;49macquire()\n\u001b[1;32m 303\u001b[0m gotit \u001b[39m=\u001b[39m \u001b[39mTrue\u001b[39;00m\n\u001b[1;32m 304\u001b[0m \u001b[39melse\u001b[39;00m:\n", - "\u001b[0;31mKeyboardInterrupt\u001b[0m: " - ] - } - ], + "outputs": [], "source": [ "cp_parallel.run_cellprofiler_parallel(\n", " plate_info_dictionary=plate_info_dictionary, run_name=run_name\n", @@ -181,7 +165,7 @@ "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", - "version": "3.8.15" + "version": "3.8.18" }, "orig_nbformat": 4 }, diff --git a/1.cellprofiler_ic/nf1_ic.sh b/1.cellprofiler_ic/nf1_ic.sh index 6119364..cda12b9 100644 --- a/1.cellprofiler_ic/nf1_ic.sh +++ b/1.cellprofiler_ic/nf1_ic.sh @@ -5,7 +5,8 @@ conda init bash # activate the main conda environment conda activate nf1_cellpainting_data -# convert the notebook into a python and run the file -jupyter nbconvert --to python \ - --FilesWriter.build_directory=scripts/ \ - --execute nf1_ic.ipynb +# convert all notebooks to python files into the scripts folder +jupyter nbconvert --to python --output-dir=scripts/ *.ipynb + +# run the python scripts +python scripts/nf1_ic.py diff --git a/1.cellprofiler_ic/nf1_quality_control.sh b/1.cellprofiler_ic/nf1_quality_control.sh new file mode 100644 index 0000000..0b04ceb --- /dev/null +++ b/1.cellprofiler_ic/nf1_quality_control.sh @@ -0,0 +1,14 @@ +#!/bin/bash + +# initialize the correct shell for your machine to allow conda to work (see README for note on shell names) +conda init bash +# activate the main conda environment +conda activate nf1_cellpainting_data + +# convert all notebooks to python files into the scripts folder in the image_quality_control folder +jupyter nbconvert --to python --output-dir=image_quality_control/scripts/ image_quality_control/*.ipynb + +# run the image quality control scripts +python image_quality_control/scripts/0.whole_image_qc.py +python image_quality_control/scripts/1.evaluate_qc.py +Rscript image_quality_control/scripts/2.qc_report.r diff --git a/1.cellprofiler_ic/NF1_illum_3channel.cppipe b/1.cellprofiler_ic/pipelines/NF1_illum_3channel.cppipe similarity index 100% rename from 1.cellprofiler_ic/NF1_illum_3channel.cppipe rename to 1.cellprofiler_ic/pipelines/NF1_illum_3channel.cppipe diff --git a/1.cellprofiler_ic/NF1_illum_4channel.cppipe b/1.cellprofiler_ic/pipelines/NF1_illum_4channel.cppipe similarity index 63% rename from 1.cellprofiler_ic/NF1_illum_4channel.cppipe rename to 1.cellprofiler_ic/pipelines/NF1_illum_4channel.cppipe index 4b3cc6e..3ae7f6a 100644 --- a/1.cellprofiler_ic/NF1_illum_4channel.cppipe +++ b/1.cellprofiler_ic/pipelines/NF1_illum_4channel.cppipe @@ -2,7 +2,7 @@ CellProfiler Pipeline: http://www.cellprofiler.org Version:5 DateRevision:424 GitHash: -ModuleCount:13 +ModuleCount:16 HasImagePlaneDetails:False Images:[module_num:1|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Images module is blank in the GUI since we are adding the image path in CLI.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] @@ -10,14 +10,14 @@ Images:[module_num:1|svn_version:'Unknown'|variable_revision_number:2|show_windo Filter images?:Images only Select the rule criteria:and (extension does isimage) (directory doesnot containregexp "[\\\\/]\\.") -Metadata:[module_num:2|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['We do not extract metadata when performing illumination correction']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] - Extract metadata?:No +Metadata:[module_num:2|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['Extract well and site metadata to use and assess which images have been flagged']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Extract metadata?:Yes Metadata data type:Text Metadata types:{} Extraction method count:1 Metadata extraction method:Extract from file/folder names Metadata source:File name - Regular expression to extract from file name:^(?P.*)_(?P[A-P][0-9]{2})_s(?P[0-9])_w(?P[0-9]) + Regular expression to extract from file name:^(?P[A-Z]{1}[0-9]{1,2})_01_(?P[0-4]{1})_(?P[0-9]{1,2}) Regular expression to extract from folder name:(?P[0-9]{4}_[0-9]{2}_[0-9]{2})$ Extract metadata from:All images Select the filtering criteria:and (file does contain "") @@ -71,7 +71,123 @@ Groups:[module_num:4|svn_version:'Unknown'|variable_revision_number:2|show_windo grouping metadata count:1 Metadata category:None -CorrectIlluminationCalculate:[module_num:5|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since for all seeding densities, Schwann cells do not take up a ton of space in the images, we use Background in the IC calculations.', '', 'Block size 50 seems to work best for the DAPI channel (nuclei), as well as using the Fit Polynomial smoothing method as it seemed to not over correct as compared to the other methods.', '', 'We use the each method for calculating IC functions as this seemed to produce the best results over creating one from all images in a channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +MeasureImageQuality:[module_num:5|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['Only calculate the blur and saturation image quality metrics as we are using these to flag images for poor quality. Blur is set as default.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Calculate metrics for which images?:All loaded images + Image count:1 + Scale count:1 + Threshold count:1 + Select the images to measure: + Include the image rescaling value?:Yes + Calculate blur metrics?:Yes + Spatial scale for blur measurements:20 + Calculate saturation metrics?:Yes + Calculate intensity metrics?:No + Calculate thresholds?:No + Use all thresholding methods?:No + Select a thresholding method:Otsu + Typical fraction of the image covered by objects:0.1 + Two-class or three-class thresholding?:Two classes + Minimize the weighted variance or the entropy?:Weighted variance + Assign pixels in the middle intensity class to the foreground or the background?:Foreground + +FlagImage:[module_num:6|svn_version:'Unknown'|variable_revision_number:5|show_window:False|notes:['We use blur and saturation to detect poor quality images. Either they are not processed for being out-of-focus or empty depending on the blur metric or over saturated/contain large smudge from the saturation metric. We are setting this as flag if any fail since these are not dependent on each other to make an image poor quality.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Hidden:1 + Hidden:8 + Name the flag's category:Image_Quality_Control + Name the flag:QC_Flag + How should measurements be linked?:Flag if any fail + Skip image set if flagged?:Yes + Flag is based on:Whole-image measurement + Select the object to be used for flagging:None + Which measurement?:ImageQuality_PowerLogLogSlope_OrigDAPI + Flag images based on low values?:Yes + Minimum value:-2.50 + Flag images based on high values?:Yes + Maximum value:-1.37 + Rules file location:Elsewhere...| + Rules file name:rules.txt + Class number: + Allow fuzzy feature matching?:No + Flag is based on:Whole-image measurement + Select the object to be used for flagging:None + Which measurement?:ImageQuality_PowerLogLogSlope_OrigGFP + Flag images based on low values?:Yes + Minimum value:-2.50 + Flag images based on high values?:Yes + Maximum value:-1.37 + Rules file location:Elsewhere...| + Rules file name:rules.txt + Class number: + Allow fuzzy feature matching?:No + Flag is based on:Whole-image measurement + Select the object to be used for flagging:None + Which measurement?:ImageQuality_PowerLogLogSlope_OrigMito + Flag images based on low values?:Yes + Minimum value:-2.50 + Flag images based on high values?:Yes + Maximum value:-1.37 + Rules file location:Elsewhere...| + Rules file name:rules.txt + Class number: + Allow fuzzy feature matching?:No + Flag is based on:Whole-image measurement + Select the object to be used for flagging:None + Which measurement?:ImageQuality_PowerLogLogSlope_OrigRFP + Flag images based on low values?:Yes + Minimum value:-2.50 + Flag images based on high values?:Yes + Maximum value:-1.37 + Rules file location:Elsewhere...| + Rules file name:rules.txt + Class number: + Allow fuzzy feature matching?:No + Flag is based on:Whole-image measurement + Select the object to be used for flagging:None + Which measurement?:ImageQuality_PercentMaximal_OrigDAPI + Flag images based on low values?:No + Minimum value:0.0 + Flag images based on high values?:Yes + Maximum value:1.40 + Rules file location:Elsewhere...| + Rules file name:rules.txt + Class number: + Allow fuzzy feature matching?:No + Flag is based on:Whole-image measurement + Select the object to be used for flagging:None + Which measurement?:ImageQuality_PercentMaximal_OrigGFP + Flag images based on low values?:No + Minimum value:0.0 + Flag images based on high values?:Yes + Maximum value:1.40 + Rules file location:Elsewhere...| + Rules file name:rules.txt + Class number: + Allow fuzzy feature matching?:No + Flag is based on:Whole-image measurement + Select the object to be used for flagging:None + Which measurement?:ImageQuality_PercentMaximal_OrigMito + Flag images based on low values?:No + Minimum value:0.0 + Flag images based on high values?:Yes + Maximum value:1.40 + Rules file location:Elsewhere...| + Rules file name:rules.txt + Class number: + Allow fuzzy feature matching?:No + Flag is based on:Whole-image measurement + Select the object to be used for flagging:None + Which measurement?:ImageQuality_PercentMaximal_OrigRFP + Flag images based on low values?:No + Minimum value:0.0 + Flag images based on high values?:Yes + Maximum value:1.40 + Rules file location:Elsewhere...| + Rules file name:rules.txt + Class number: + Allow fuzzy feature matching?:No + Ignore flag skips on last cycle?:No + +CorrectIlluminationCalculate:[module_num:7|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since for all seeding densities, Schwann cells do not take up a ton of space in the images, we use Background in the IC calculations.', '', 'Block size 50 seems to work best for the DAPI channel (nuclei), as well as using the Fit Polynomial smoothing method as it seemed to not over correct as compared to the other methods.', '', 'We use the each method for calculating IC functions as this seemed to produce the best results over creating one from all images in a channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the input image:OrigDAPI Name the output image:IllumDAPI Select how the illumination function is calculated:Background @@ -96,7 +212,7 @@ CorrectIlluminationCalculate:[module_num:5|svn_version:'Unknown'|variable_revisi Maximum number of iterations:40 Residual value for convergence:0.001 -CorrectIlluminationCalculate:[module_num:6|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since for all seeding densities, Schwann cells do not take up a ton of space in the images, we use Background in the IC calculations.', '', 'Block size 50 seems to work best for the GFP channel (ER), as well as using the Fit Polynomial smoothing method as it seemed to not over correct as compared to the other methods.', '', 'We use the each method for calculating IC functions as this seemed to produce the best results over creating one from all images in a channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +CorrectIlluminationCalculate:[module_num:8|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since for all seeding densities, Schwann cells do not take up a ton of space in the images, we use Background in the IC calculations.', '', 'Block size 50 seems to work best for the GFP channel (ER), as well as using the Fit Polynomial smoothing method as it seemed to not over correct as compared to the other methods.', '', 'We use the each method for calculating IC functions as this seemed to produce the best results over creating one from all images in a channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the input image:OrigGFP Name the output image:IllumGFP Select how the illumination function is calculated:Background @@ -121,7 +237,7 @@ CorrectIlluminationCalculate:[module_num:6|svn_version:'Unknown'|variable_revisi Maximum number of iterations:40 Residual value for convergence:0.001 -CorrectIlluminationCalculate:[module_num:7|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since for all seeding densities, Schwann cells do not take up a ton of space in the images, we use Background in the IC calculations.', '', 'Block size 15 seems to work best for the RFP channel (actin), as well as using the Fit Polynomial smoothing method as it seemed to not over correct as compared to the other methods.', '', 'We use the each method for calculating IC functions as this seemed to produce the best results over creating one from all images in a channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +CorrectIlluminationCalculate:[module_num:9|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since for all seeding densities, Schwann cells do not take up a ton of space in the images, we use Background in the IC calculations.', '', 'Block size 15 seems to work best for the RFP channel (actin), as well as using the Fit Polynomial smoothing method as it seemed to not over correct as compared to the other methods.', '', 'We use the each method for calculating IC functions as this seemed to produce the best results over creating one from all images in a channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the input image:OrigRFP Name the output image:IllumRFP Select how the illumination function is calculated:Background @@ -146,7 +262,7 @@ CorrectIlluminationCalculate:[module_num:7|svn_version:'Unknown'|variable_revisi Maximum number of iterations:40 Residual value for convergence:0.001 -CorrectIlluminationCalculate:[module_num:8|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since for all seeding densities, Schwann cells do not take up a ton of space in the images, we use Background in the IC calculations.', '', 'Block size 15 seems to work best for the CY5 channel (mitochondria), as well as using the Fit Polynomial smoothing method as it seemed to not over correct as compared to the other methods.', '', 'We use the each method for calculating IC functions as this seemed to produce the best results over creating one from all images in a channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +CorrectIlluminationCalculate:[module_num:10|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since for all seeding densities, Schwann cells do not take up a ton of space in the images, we use Background in the IC calculations.', '', 'Block size 15 seems to work best for the CY5 channel (mitochondria), as well as using the Fit Polynomial smoothing method as it seemed to not over correct as compared to the other methods.', '', 'We use the each method for calculating IC functions as this seemed to produce the best results over creating one from all images in a channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the input image:OrigMito Name the output image:IllumMito Select how the illumination function is calculated:Background @@ -171,7 +287,7 @@ CorrectIlluminationCalculate:[module_num:8|svn_version:'Unknown'|variable_revisi Maximum number of iterations:40 Residual value for convergence:0.001 -CorrectIlluminationApply:[module_num:9|svn_version:'Unknown'|variable_revision_number:5|show_window:False|notes:['Apply illumination correction functions to all channels']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +CorrectIlluminationApply:[module_num:11|svn_version:'Unknown'|variable_revision_number:5|show_window:False|notes:['Apply illumination correction functions to all channels']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the input image:OrigDAPI Name the output image:CorrDAPI Select the illumination function:IllumDAPI @@ -191,7 +307,7 @@ CorrectIlluminationApply:[module_num:9|svn_version:'Unknown'|variable_revision_n Set output image values less than 0 equal to 0?:Yes Set output image values greater than 1 equal to 1?:Yes -SaveImages:[module_num:10|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:['Save DAPI images as same bit-depth']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +SaveImages:[module_num:12|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:['Save DAPI images as same bit-depth']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the type of image to save:Image Select the image to save:CorrDAPI Select method for constructing file names:From image filename @@ -211,7 +327,7 @@ SaveImages:[module_num:10|svn_version:'Unknown'|variable_revision_number:16|show How to save the series:T (Time) Save with lossless compression?:Yes -SaveImages:[module_num:11|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:['Save GFP images as same bit-depth']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +SaveImages:[module_num:13|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:['Save GFP images as same bit-depth']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the type of image to save:Image Select the image to save:CorrGFP Select method for constructing file names:From image filename @@ -231,7 +347,7 @@ SaveImages:[module_num:11|svn_version:'Unknown'|variable_revision_number:16|show How to save the series:T (Time) Save with lossless compression?:Yes -SaveImages:[module_num:12|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:['Save RFP images as same bit-depth']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +SaveImages:[module_num:14|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:['Save RFP images as same bit-depth']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the type of image to save:Image Select the image to save:CorrRFP Select method for constructing file names:From image filename @@ -251,7 +367,7 @@ SaveImages:[module_num:12|svn_version:'Unknown'|variable_revision_number:16|show How to save the series:T (Time) Save with lossless compression?:Yes -SaveImages:[module_num:13|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:['Save CY5 images as the same bit-depth']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] +SaveImages:[module_num:15|svn_version:'Unknown'|variable_revision_number:16|show_window:False|notes:['Save CY5 images as the same bit-depth']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] Select the type of image to save:Image Select the image to save:CorrMito Select method for constructing file names:From image filename @@ -270,3 +386,27 @@ SaveImages:[module_num:13|svn_version:'Unknown'|variable_revision_number:16|show Base image folder:Elsewhere...| How to save the series:T (Time) Save with lossless compression?:Yes + +ExportToSpreadsheet:[module_num:16|svn_version:'Unknown'|variable_revision_number:13|show_window:False|notes:[]|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the column delimiter:Comma (",") + Add image metadata columns to your object data file?:No + Add image file and folder names to your object data file?:No + Select the measurements to export:No + Calculate the per-image mean values for object measurements?:No + Calculate the per-image median values for object measurements?:No + Calculate the per-image standard deviation values for object measurements?:No + Output file location:Default Output Folder| + Create a GenePattern GCT file?:No + Select source of sample row name:Metadata + Select the image to use as the identifier:None + Select the metadata to use as the identifier:None + Export all measurement types?:Yes + Press button to select measurements: + Representation of Nan/Inf:NaN + Add a prefix to file names?:Yes + Filename prefix:IC_QC_Run + Overwrite existing files without warning?:No + Data to export:Image + Combine these object measurements with those of the previous object?:No + File name:DATA.csv + Use the object name for the file name?:Yes diff --git a/1.cellprofiler_ic/pipelines/whole_image_qc.cppipe b/1.cellprofiler_ic/pipelines/whole_image_qc.cppipe new file mode 100644 index 0000000..881eb3f --- /dev/null +++ b/1.cellprofiler_ic/pipelines/whole_image_qc.cppipe @@ -0,0 +1,115 @@ +CellProfiler Pipeline: http://www.cellprofiler.org +Version:5 +DateRevision:424 +GitHash: +ModuleCount:6 +HasImagePlaneDetails:False + +Images:[module_num:1|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Add the plate images to view when testing parameters. If not, make sure it is blank prior to processing the images via the notebook.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + : + Filter images?:Images only + Select the rule criteria:and (extension does isimage) (directory doesnot containregexp "[\\\\/]\\.") + +Metadata:[module_num:2|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['There is no need to extract metadata since we are not using the GUI to processes each plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Extract metadata?:No + Metadata data type:Text + Metadata types:{} + Extraction method count:1 + Metadata extraction method:Extract from file/folder names + Metadata source:Folder name + Regular expression to extract from file name:^(?P.*)_(?P[A-P][0-9]{2})_s(?P[0-9])_w(?P[0-9]) + Regular expression to extract from folder name:(?Plocalhost220512140003_KK22-05-198|localhost220513100001_KK22-05-198_FactinAdjusted|localhost230405150001) + Extract metadata from:All images + Select the filtering criteria:and (file does contain "") + Metadata file location:Elsewhere...| + Match file and image metadata:[] + Use case insensitive matching?:No + Metadata file name:None + Does cached metadata exist?:No + +NamesAndTypes:[module_num:3|svn_version:'Unknown'|variable_revision_number:8|show_window:False|notes:['Assign each image to its channel.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Assign a name to:Images matching rules + Select the image type:Grayscale image + Name to assign these images:DNA + Match metadata:[] + Image set matching method:Order + Set intensity range from:Image metadata + Assignments count:4 + Single images count:0 + Maximum intensity:255.0 + Process as 3D?:No + Relative pixel spacing in X:1.0 + Relative pixel spacing in Y:1.0 + Relative pixel spacing in Z:1.0 + Select the rule criteria:and (file does contain "DAPI") + Name to assign these images:OrigDAPI + Name to assign these objects:Cell + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain "GFP") + Name to assign these images:OrigGFP + Name to assign these objects:Nucleus + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain "RFP") + Name to assign these images:OrigRFP + Name to assign these objects:Cytoplasm + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + Select the rule criteria:and (file does contain "CY5") + Name to assign these images:OrigCY5 + Name to assign these objects:Speckle + Select the image type:Grayscale image + Set intensity range from:Image metadata + Maximum intensity:255.0 + +Groups:[module_num:4|svn_version:'Unknown'|variable_revision_number:2|show_window:False|notes:['Since we are not using the GUI to processes each plate, we do NOT need to group the images by plate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Do you want to group your images?:No + grouping metadata count:1 + Metadata category:Plate + +MeasureImageQuality:[module_num:5|svn_version:'Unknown'|variable_revision_number:6|show_window:False|notes:['To measure image quality, we will only be using blur and saturation to determine how out of focus images are and if there are large highly saturated artifacts. Defaults are used for blur.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Calculate metrics for which images?:All loaded images + Image count:1 + Scale count:1 + Threshold count:1 + Select the images to measure: + Include the image rescaling value?:Yes + Calculate blur metrics?:Yes + Spatial scale for blur measurements:20 + Calculate saturation metrics?:Yes + Calculate intensity metrics?:No + Calculate thresholds?:No + Use all thresholding methods?:No + Select a thresholding method:Otsu + Typical fraction of the image covered by objects:0.1 + Two-class or three-class thresholding?:Two classes + Minimize the weighted variance or the entropy?:Weighted variance + Assign pixels in the middle intensity class to the foreground or the background?:Foreground + +ExportToSpreadsheet:[module_num:6|svn_version:'Unknown'|variable_revision_number:13|show_window:False|notes:['Export all of the whole image quality metrics to a spreadsheet to load in and evaluate.']|batch_state:array([], dtype=uint8)|enabled:True|wants_pause:False] + Select the column delimiter:Comma (",") + Add image metadata columns to your object data file?:No + Add image file and folder names to your object data file?:No + Select the measurements to export:No + Calculate the per-image mean values for object measurements?:No + Calculate the per-image median values for object measurements?:No + Calculate the per-image standard deviation values for object measurements?:No + Output file location:Default Output Folder| + Create a GenePattern GCT file?:No + Select source of sample row name:Metadata + Select the image to use as the identifier:None + Select the metadata to use as the identifier:None + Export all measurement types?:Yes + Press button to select measurements: + Representation of Nan/Inf:NaN + Add a prefix to file names?:No + Filename prefix:Whole_Image_QC + Overwrite existing files without warning?:No + Data to export:Do not use + Combine these object measurements with those of the previous object?:No + File name:DATA.csv + Use the object name for the file name?:Yes diff --git a/1.cellprofiler_ic/scripts/nf1_ic.py b/1.cellprofiler_ic/scripts/nf1_ic.py index 76c2c26..10dbb89 100644 --- a/1.cellprofiler_ic/scripts/nf1_ic.py +++ b/1.cellprofiler_ic/scripts/nf1_ic.py @@ -25,8 +25,8 @@ # set the run type for the parallelization run_name = "illum_correction" -# set path for pipeline for all plates for whole image analysis -path_to_pipeline = pathlib.Path("./pipelines/nuclei_analysis.cppipe").resolve() +# Directory with pipelines +pipeline_dir = pathlib.Path("./pipelines/").resolve(strict=True) # set main output dir for all plates output_dir = pathlib.Path("./Corrected_Images") @@ -65,12 +65,12 @@ for name, info in plate_info_dictionary.items(): # only plates 1 and 2 have 3 channels so these are the only plates that use this path if name == "Plate_1" or name == "Plate_2": - info["path_to_pipeline"] = pathlib.Path(f"./NF1_illum_3channel.cppipe").resolve( + info["path_to_pipeline"] = pathlib.Path(f"{pipeline_dir}/NF1_illum_3channel.cppipe").resolve( strict=True ) # all other plates have 4 channels and will use that specific pipeline else: - info["path_to_pipeline"] = pathlib.Path(f"./NF1_illum_4channel.cppipe").resolve( + info["path_to_pipeline"] = pathlib.Path(f"{pipeline_dir}/NF1_illum_4channel.cppipe").resolve( strict=True ) @@ -82,7 +82,7 @@ # # In this notebook, we do not run the cells to completion as we prefer to run the notebooks as nbconverted python files due to better stability. -# In[4]: +# In[ ]: cp_parallel.run_cellprofiler_parallel( diff --git a/3.processing_features/1.pycytominer_bulk_pipelines.ipynb b/3.processing_features/1.pycytominer_bulk_pipelines.ipynb index 91c67c3..179ff55 100644 --- a/3.processing_features/1.pycytominer_bulk_pipelines.ipynb +++ b/3.processing_features/1.pycytominer_bulk_pipelines.ipynb @@ -381,7 +381,7 @@ "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", - "version": "3.8.15" + "version": "3.8.18" } }, "nbformat": 4, diff --git a/3.processing_features/2.pycytominer_singlecell_pipelines.ipynb b/3.processing_features/2.pycytominer_singlecell_pipelines.ipynb index 2b34254..1ce6747 100644 --- a/3.processing_features/2.pycytominer_singlecell_pipelines.ipynb +++ b/3.processing_features/2.pycytominer_singlecell_pipelines.ipynb @@ -239,7 +239,7 @@ "name": "python", "nbconvert_exporter": "python", "pygments_lexer": "ipython3", - "version": "3.10.8" + "version": "3.8.18" } }, "nbformat": 4, From 9ebdf0ed536907edf715bb9a24eda82e43804504 Mon Sep 17 00:00:00 2001 From: jenna-tomkinson Date: Wed, 7 Feb 2024 09:34:28 -0700 Subject: [PATCH 4/4] edit code --- .../image_quality_control/1.evaluate_qc.ipynb | 131 +++++++++++++++--- .../image_quality_control/2.qc_report.ipynb | 27 ++-- .../Plate_5_per_site_flagged_fov.png | Bin 102135 -> 112169 bytes .../scripts/1.evaluate_qc.py | 8 +- .../scripts/{2.qc_report.py => 2.qc_report.r} | 54 +------- 1.cellprofiler_ic/nf1_quality_control.sh | 24 +++- 6 files changed, 153 insertions(+), 91 deletions(-) rename 1.cellprofiler_ic/image_quality_control/scripts/{2.qc_report.py => 2.qc_report.r} (87%) diff --git a/1.cellprofiler_ic/image_quality_control/1.evaluate_qc.ipynb b/1.cellprofiler_ic/image_quality_control/1.evaluate_qc.ipynb index ebe6e9e..e7ee45b 100644 --- a/1.cellprofiler_ic/image_quality_control/1.evaluate_qc.ipynb +++ b/1.cellprofiler_ic/image_quality_control/1.evaluate_qc.ipynb @@ -488,12 +488,12 @@ " for plate, qc_df in all_qc_data_frames.items():\n", " plate_df = qc_df.filter(like=\"Metadata_\").copy()\n", "\n", - " # Add PowerLogLogSlope column\n", + " # Add PowerLogLogSlope column (blur metric)\n", " plate_df[f\"ImageQuality_PowerLogLogSlope\"] = qc_df[\n", " f\"ImageQuality_PowerLogLogSlope_Orig{channel}\"\n", " ]\n", "\n", - " # Add PercentMaximal column\n", + " # Add PercentMaximal column (saturation metric)\n", " plate_df[f\"ImageQuality_PercentMaximal\"] = qc_df[\n", " f\"ImageQuality_PercentMaximal_Orig{channel}\"\n", " ]\n", @@ -669,31 +669,118 @@ " DAPI\n", " Plate_5\n", " \n", + " \n", + " ...\n", + " ...\n", + " ...\n", + " ...\n", + " ...\n", + " ...\n", + " ...\n", + " ...\n", + " ...\n", + " \n", + " \n", + " 20978\n", + " 0\n", + " 0\n", + " E7\n", + " 2\n", + " -0.458892\n", + " 0.000090\n", + " CY5\n", + " Plate_3\n", + " \n", + " \n", + " 22921\n", + " 0\n", + " 0\n", + " F1\n", + " 1\n", + " -0.358638\n", + " 0.000090\n", + " CY5\n", + " Plate_3_prime\n", + " \n", + " \n", + " 6703\n", + " 0\n", + " 0\n", + " B1\n", + " 25\n", + " -0.307717\n", + " 0.000090\n", + " GFP\n", + " Plate_3\n", + " \n", + " \n", + " 22828\n", + " 0\n", + " 0\n", + " E9\n", + " 2\n", + " -0.209380\n", + " 0.000090\n", + " CY5\n", + " Plate_3_prime\n", + " \n", + " \n", + " 20841\n", + " 0\n", + " 0\n", + " E2\n", + " 15\n", + " -0.192534\n", + " 0.000090\n", + " CY5\n", + " Plate_3\n", + " \n", " \n", "\n", + "

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