diff --git a/airflow-core/src/airflow/ui/src/components/LimitedItemsList.tsx b/airflow-core/src/airflow/ui/src/components/LimitedItemsList.tsx
index 7d993413c2dff..3ff3b85638950 100644
--- a/airflow-core/src/airflow/ui/src/components/LimitedItemsList.tsx
+++ b/airflow-core/src/airflow/ui/src/components/LimitedItemsList.tsx
@@ -57,7 +57,8 @@ export const LimitedItemsList = ({
           // eslint-disable-next-line react/no-array-index-key
           <React.Fragment key={index}>
             <Text as="span">{item}</Text>
-            {index < displayItems.length - 1 || (shouldTruncate && remainingItems.length > 1) ? (
+            {index < displayItems.length - 1 ||
+            (shouldTruncate && remainingItems.length >= 1 && index === displayItems.length - 1) ? (
               <Text as="span">{separator}</Text>
             ) : undefined}
           </React.Fragment>
diff --git a/airflow-core/src/airflow/ui/src/pages/DagsList/DagCard.test.tsx b/airflow-core/src/airflow/ui/src/pages/DagsList/DagCard.test.tsx
index 0d44614cab2be..fc822119d3a4b 100644
--- a/airflow-core/src/airflow/ui/src/pages/DagsList/DagCard.test.tsx
+++ b/airflow-core/src/airflow/ui/src/pages/DagsList/DagCard.test.tsx
@@ -82,6 +82,7 @@ describe("DagCard", () => {
 
     render(<DagCard dag={expandedMockDag} />, { wrapper: Wrapper });
     expect(screen.getByTestId("dag-tag")).toBeInTheDocument();
+    expect(screen.queryByText("tag3")).toBeInTheDocument();
     expect(screen.queryByText("tag4")).toBeInTheDocument();
     expect(screen.queryByText(", +1 more")).toBeNull();
   });