diff --git a/airflow-core/src/airflow/api_fastapi/app.py b/airflow-core/src/airflow/api_fastapi/app.py
index 2d2afef0f2609..36b59630d0b65 100644
--- a/airflow-core/src/airflow/api_fastapi/app.py
+++ b/airflow-core/src/airflow/api_fastapi/app.py
@@ -170,7 +170,6 @@ def init_plugins(app: FastAPI) -> None:
     """Integrate FastAPI app, middlewares and UI plugins."""
     from airflow import plugins_manager
 
-    plugins_manager.initialize_fastapi_plugins()
     plugins_manager.initialize_fastapi_plugins()
 
     # After calling initialize_fastapi_plugins, fastapi_apps cannot be None anymore.