diff --git a/airflow-core/src/airflow/utils/log/file_task_handler.py b/airflow-core/src/airflow/utils/log/file_task_handler.py
index 4dc9051c02559..3669aae122b3d 100644
--- a/airflow-core/src/airflow/utils/log/file_task_handler.py
+++ b/airflow-core/src/airflow/utils/log/file_task_handler.py
@@ -175,6 +175,9 @@ def _fetch_logs_from_service(url: str, log_relative_path: str) -> Response:
         secret_key=get_signing_key("api", "secret_key"),
         # Since we are using a secret key, we need to be explicit about the algorithm here too
         algorithm="HS512",
+        # We must set an empty private key here as otherwise it can be automatically loaded by JWTGenerator
+        # and secret_key and private_key cannot be set together
+        private_key=None,  #  type: ignore[arg-type]
         issuer=None,
         valid_for=conf.getint("webserver", "log_request_clock_grace", fallback=30),
         audience="task-instance-logs",