diff --git a/airflow-core/src/airflow/models/serialized_dag.py b/airflow-core/src/airflow/models/serialized_dag.py
index 71722b54adee6..684cea847791b 100644
--- a/airflow-core/src/airflow/models/serialized_dag.py
+++ b/airflow-core/src/airflow/models/serialized_dag.py
@@ -350,7 +350,13 @@ def __repr__(self) -> str:
     def hash(cls, dag_data):
         """Hash the data to get the dag_hash."""
         dag_data = cls._sort_serialized_dag_dict(dag_data)
-        data_json = json.dumps(dag_data, sort_keys=True).encode("utf-8")
+        data_ = dag_data.copy()
+        # Remove fileloc from the hash so changes to fileloc
+        # does not affect the hash. In 3.0+, a combination of
+        # bundle_path and relative fileloc more correctly determines the
+        # dag file location.
+        data_["dag"].pop("fileloc", None)
+        data_json = json.dumps(data_, sort_keys=True).encode("utf-8")
         return md5(data_json).hexdigest()
 
     @classmethod
diff --git a/airflow-core/tests/unit/models/test_serialized_dag.py b/airflow-core/tests/unit/models/test_serialized_dag.py
index d30d71d0b02cb..f6277038dd96c 100644
--- a/airflow-core/tests/unit/models/test_serialized_dag.py
+++ b/airflow-core/tests/unit/models/test_serialized_dag.py
@@ -522,3 +522,31 @@ def test_new_dag_version_created_when_bundle_name_changes_and_hash_unchanged(sel
 
         # There should now be two versions of the DAG
         assert session.query(DagVersion).count() == 2
+
+    def test_hash_method_removes_fileloc_and_remains_consistent(self):
+        """Test that the hash method removes fileloc before hashing."""
+        test_data = {
+            "__version": 1,
+            "dag": {
+                "fileloc": "/path/to/dag.py",
+                "dag_id": "test_dag",
+                "tasks": {
+                    "task1": {"task_id": "task1"},
+                },
+            },
+        }
+
+        hash_with_fileloc = SDM.hash(test_data)
+
+        # Modify only the top-level dag.fileloc path (simulating file location changes)
+        test_data["dag"]["fileloc"] = "/different/path/to/dag.py"
+
+        # Get hash with different top-level fileloc (should be the same)
+        hash_with_different_fileloc = SDM.hash(test_data)
+
+        # Hashes should be identical since top-level dag.fileloc is removed before hashing
+        assert hash_with_fileloc == hash_with_different_fileloc
+
+        # Verify that the original data still has fileloc (method shouldn't modify original)
+        assert "fileloc" in test_data["dag"]
+        assert test_data["dag"]["fileloc"] == "/different/path/to/dag.py"