diff --git a/airflow-core/tests/unit/cli/commands/test_db_command.py b/airflow-core/tests/unit/cli/commands/test_db_command.py
index cd7d61f5f2467..363dd21b2ea25 100644
--- a/airflow-core/tests/unit/cli/commands/test_db_command.py
+++ b/airflow-core/tests/unit/cli/commands/test_db_command.py
@@ -694,7 +694,7 @@ def test_date_timezone_omitted(self, run_cleanup_mock, timezone):
         coerced to tz-aware with default timezone
         """
         timestamp = "2021-01-01 00:00:00"
-        with patch("airflow.settings.TIMEZONE", pendulum.timezone(timezone)):
+        with patch("airflow.settings._Timezone.initialized_timezone", pendulum.timezone(timezone)):
             args = self.parser.parse_args(["db", "clean", "--clean-before-timestamp", f"{timestamp}", "-y"])
             db_command.cleanup_tables(args)
         run_cleanup_mock.assert_called_once_with(