diff --git a/airflow-core/src/airflow/api_fastapi/core_api/datamodels/pools.py b/airflow-core/src/airflow/api_fastapi/core_api/datamodels/pools.py
index 0e56684081a73..077ddbe550552 100644
--- a/airflow-core/src/airflow/api_fastapi/core_api/datamodels/pools.py
+++ b/airflow-core/src/airflow/api_fastapi/core_api/datamodels/pools.py
@@ -20,7 +20,7 @@
 from collections.abc import Callable, Iterable
 from typing import Annotated
 
-from pydantic import BeforeValidator, Field
+from pydantic import BeforeValidator, Field, PositiveInt
 
 from airflow.api_fastapi.core_api.base import BaseModel, StrictBaseModel
 
@@ -38,7 +38,7 @@ class BasePool(BaseModel):
     """Base serializer for Pool."""
 
     pool: str = Field(serialization_alias="name")
-    slots: int
+    slots: PositiveInt
     description: str | None = Field(default=None)
     include_deferred: bool
 
@@ -66,7 +66,7 @@ class PoolPatchBody(StrictBaseModel):
     """Pool serializer for patch bodies."""
 
     name: str | None = Field(default=None, alias="pool")
-    slots: int | None = None
+    slots: PositiveInt | None = None
     description: str | None = None
     include_deferred: bool | None = None
     team_name: str | None = Field(max_length=50, default=None)
diff --git a/airflow-core/src/airflow/api_fastapi/core_api/openapi/v2-rest-api-generated.yaml b/airflow-core/src/airflow/api_fastapi/core_api/openapi/v2-rest-api-generated.yaml
index 9993e05efc688..03a62cd64b286 100644
--- a/airflow-core/src/airflow/api_fastapi/core_api/openapi/v2-rest-api-generated.yaml
+++ b/airflow-core/src/airflow/api_fastapi/core_api/openapi/v2-rest-api-generated.yaml
@@ -12041,6 +12041,7 @@ components:
           title: Name
         slots:
           type: integer
+          exclusiveMinimum: 0.0
           title: Slots
         description:
           anyOf:
@@ -12090,6 +12091,7 @@ components:
         slots:
           anyOf:
           - type: integer
+            exclusiveMinimum: 0.0
           - type: 'null'
           title: Slots
         description:
@@ -12119,6 +12121,7 @@ components:
           title: Name
         slots:
           type: integer
+          exclusiveMinimum: 0.0
           title: Slots
         description:
           anyOf:
diff --git a/airflow-core/src/airflow/ui/openapi-gen/requests/schemas.gen.ts b/airflow-core/src/airflow/ui/openapi-gen/requests/schemas.gen.ts
index 660742644e619..54465d540f09e 100644
--- a/airflow-core/src/airflow/ui/openapi-gen/requests/schemas.gen.ts
+++ b/airflow-core/src/airflow/ui/openapi-gen/requests/schemas.gen.ts
@@ -4597,6 +4597,7 @@ export const $PoolBody = {
         },
         slots: {
             type: 'integer',
+            exclusiveMinimum: 0,
             title: 'Slots'
         },
         description: {
@@ -4671,7 +4672,8 @@ export const $PoolPatchBody = {
         slots: {
             anyOf: [
                 {
-                    type: 'integer'
+                    type: 'integer',
+                    exclusiveMinimum: 0
                 },
                 {
                     type: 'null'
@@ -4728,6 +4730,7 @@ export const $PoolResponse = {
         },
         slots: {
             type: 'integer',
+            exclusiveMinimum: 0,
             title: 'Slots'
         },
         description: {
diff --git a/airflow-core/tests/unit/api_fastapi/core_api/routes/public/test_pools.py b/airflow-core/tests/unit/api_fastapi/core_api/routes/public/test_pools.py
index 6d8d924ae2b78..bb34660f0e02f 100644
--- a/airflow-core/tests/unit/api_fastapi/core_api/routes/public/test_pools.py
+++ b/airflow-core/tests/unit/api_fastapi/core_api/routes/public/test_pools.py
@@ -285,6 +285,24 @@ class TestPatchPool(TestPoolsEndpoint):
                     ],
                 },
             ),
+            # Negative slot number
+            (
+                POOL1_NAME,
+                {},
+                {"slots": -10},
+                422,
+                {
+                    "detail": [
+                        {
+                            "ctx": {"gt": 0},
+                            "input": -10,
+                            "loc": ["body", "slots"],
+                            "msg": "Input should be greater than 0",
+                            "type": "greater_than",
+                        },
+                    ],
+                },
+            ),
             # Partial body on default_pool
             (
                 Pool.DEFAULT_POOL_NAME,
@@ -364,7 +382,8 @@ def test_should_respond_200(
         if response.status_code == 422:
             for error in body["detail"]:
                 # pydantic version can vary in tests (lower constraints), we do not assert the url.
-                del error["url"]
+                if "url" in error:
+                    del error["url"]
 
         assert body == expected_response
         if response.status_code == 200:
diff --git a/airflow-ctl/src/airflowctl/api/datamodels/generated.py b/airflow-ctl/src/airflowctl/api/datamodels/generated.py
index ed5c0e4784383..4a653867a6c91 100644
--- a/airflow-ctl/src/airflowctl/api/datamodels/generated.py
+++ b/airflow-ctl/src/airflowctl/api/datamodels/generated.py
@@ -633,12 +633,16 @@ class PoolBody(BaseModel):
         extra="forbid",
     )
     name: Annotated[str, Field(max_length=256, title="Name")]
-    slots: Annotated[int, Field(title="Slots")]
+    slots: Annotated[int, Field(gt=0, title="Slots")]
     description: Annotated[str | None, Field(title="Description")] = None
     include_deferred: Annotated[bool | None, Field(title="Include Deferred")] = False
     team_name: Annotated[TeamName | None, Field(title="Team Name")] = None
 
 
+class Slots(RootModel[int]):
+    root: Annotated[int, Field(gt=0, title="Slots")]
+
+
 class PoolPatchBody(BaseModel):
     """
     Pool serializer for patch bodies.
@@ -648,7 +652,7 @@ class PoolPatchBody(BaseModel):
         extra="forbid",
     )
     pool: Annotated[str | None, Field(title="Pool")] = None
-    slots: Annotated[int | None, Field(title="Slots")] = None
+    slots: Annotated[Slots | None, Field(title="Slots")] = None
     description: Annotated[str | None, Field(title="Description")] = None
     include_deferred: Annotated[bool | None, Field(title="Include Deferred")] = None
     team_name: Annotated[TeamName | None, Field(title="Team Name")] = None
@@ -660,7 +664,7 @@ class PoolResponse(BaseModel):
     """
 
     name: Annotated[str, Field(title="Name")]
-    slots: Annotated[int, Field(title="Slots")]
+    slots: Annotated[int, Field(gt=0, title="Slots")]
     description: Annotated[str | None, Field(title="Description")] = None
     include_deferred: Annotated[bool, Field(title="Include Deferred")]
     occupied_slots: Annotated[int, Field(title="Occupied Slots")]