diff --git a/CHANGELOG.md b/CHANGELOG.md
index dc762fe448..37a9e710a1 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -115,6 +115,7 @@ Other changes:
 * (providers) Added {obj}`py_runtime_info.site_init_template` and
   {obj}`PyRuntimeInfo.site_init_template` for specifying the template to use to
   initialize the interpreter via venv startup hooks.
+* (runfiles) (Bazel 7.4+) Added support for spaces and newlines in runfiles paths
 
 {#v0-0-0-removed}
 ### Removed
diff --git a/MODULE.bazel b/MODULE.bazel
index 104ae92b89..2ae3173094 100644
--- a/MODULE.bazel
+++ b/MODULE.bazel
@@ -74,7 +74,7 @@ use_repo(pip, "rules_python_publish_deps")
 bazel_dep(name = "stardoc", version = "0.7.2", repo_name = "io_bazel_stardoc")
 
 # ===== DEV ONLY DEPS AND SETUP BELOW HERE =====
-bazel_dep(name = "rules_bazel_integration_test", version = "0.26.1", dev_dependency = True)
+bazel_dep(name = "rules_bazel_integration_test", version = "0.27.0", dev_dependency = True)
 bazel_dep(name = "rules_testing", version = "0.6.0", dev_dependency = True)
 bazel_dep(name = "rules_shell", version = "0.3.0", dev_dependency = True)
 bazel_dep(name = "rules_multirun", version = "0.9.0", dev_dependency = True)
diff --git a/python/runfiles/runfiles.py b/python/runfiles/runfiles.py
index 6d47d249b4..ea816c64fd 100644
--- a/python/runfiles/runfiles.py
+++ b/python/runfiles/runfiles.py
@@ -58,13 +58,24 @@ def _LoadRunfiles(path: str) -> Dict[str, str]:
         result = {}
         with open(path, "r") as f:
             for line in f:
-                line = line.strip()
-                if line:
-                    tokens = line.split(" ", 1)
-                    if len(tokens) == 1:
-                        result[line] = line
-                    else:
-                        result[tokens[0]] = tokens[1]
+                line = line.rstrip("\n")
+                if line.startswith(" "):
+                    # In lines that start with a space, spaces, newlines, and backslashes are escaped as \s, \n, and \b in
+                    # link and newlines and backslashes are escaped in target.
+                    escaped_link, escaped_target = line[1:].split(" ", maxsplit=1)
+                    link = (
+                        escaped_link.replace(r"\s", " ")
+                        .replace(r"\n", "\n")
+                        .replace(r"\b", "\\")
+                    )
+                    target = escaped_target.replace(r"\n", "\n").replace(r"\b", "\\")
+                else:
+                    link, target = line.split(" ", maxsplit=1)
+
+                if target:
+                    result[link] = target
+                else:
+                    result[link] = link
         return result
 
     def _GetRunfilesDir(self) -> str:
diff --git a/tests/runfiles/runfiles_test.py b/tests/runfiles/runfiles_test.py
index 03350f3fff..cf6a70a020 100644
--- a/tests/runfiles/runfiles_test.py
+++ b/tests/runfiles/runfiles_test.py
@@ -185,10 +185,11 @@ def testFailsToCreateAnyRunfilesBecauseEnvvarsAreNotDefined(self) -> None:
     def testManifestBasedRlocation(self) -> None:
         with _MockFile(
             contents=[
-                "Foo/runfile1",
+                "Foo/runfile1 ",  # A trailing whitespace is always present in single entry lines.
                 "Foo/runfile2 C:/Actual Path\\runfile2",
                 "Foo/Bar/runfile3 D:\\the path\\run file 3.txt",
                 "Foo/Bar/Dir E:\\Actual Path\\Directory",
+                " Foo\\sBar\\bDir\\nNewline/runfile5 F:\\bActual Path\\bwith\\nnewline/runfile5",
             ]
         ) as mf:
             r = runfiles.CreateManifestBased(mf.Path())
@@ -205,6 +206,10 @@ def testManifestBasedRlocation(self) -> None:
                 r.Rlocation("Foo/Bar/Dir/Deeply/Nested/runfile4"),
                 "E:\\Actual Path\\Directory/Deeply/Nested/runfile4",
             )
+            self.assertEqual(
+                r.Rlocation("Foo Bar\\Dir\nNewline/runfile5"),
+                "F:\\Actual Path\\with\nnewline/runfile5",
+            )
             self.assertIsNone(r.Rlocation("unknown"))
             if RunfilesTest.IsWindows():
                 self.assertEqual(r.Rlocation("c:/foo"), "c:/foo")