diff --git a/micall/tests/microtest/2020A-GP41_S4_L001_R1_001.fastq b/micall/tests/microtest/2020A-GP41_S4_L001_R1_001.fastq
index e6929b96d..43962a9af 100644
--- a/micall/tests/microtest/2020A-GP41_S4_L001_R1_001.fastq
+++ b/micall/tests/microtest/2020A-GP41_S4_L001_R1_001.fastq
@@ -1,40 +1,40 @@
 @M01234:01:000000000-AAAAA:1:1101:2020:0001 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0002 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0003 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0004 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0005 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0006 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0007 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0008 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0009 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0010 1:N:0:1
-GTGGGAATAGGAGCTTTGTTCCTTGGGATATTCTTGGGAGCAGCAGGAAGCACTATGGGCGCAGCCTCAATGACGCTGACGGTACAGGCC
+GTGGGAATAGGAGCTCTGTTCCTTGGGATATTCTTAGGAGCAGCAGGAAGCACTATGGGCGCAGCGTCAATGACGCTGACGGTACAGGCC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
diff --git a/micall/tests/microtest/2020A-GP41_S4_L001_R2_001.fastq b/micall/tests/microtest/2020A-GP41_S4_L001_R2_001.fastq
index 95ebe813a..c593705af 100644
--- a/micall/tests/microtest/2020A-GP41_S4_L001_R2_001.fastq
+++ b/micall/tests/microtest/2020A-GP41_S4_L001_R2_001.fastq
@@ -1,40 +1,40 @@
 @M01234:01:000000000-AAAAA:1:1101:2020:0001 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0002 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0003 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0004 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0005 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0006 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0007 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0008 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0009 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
 @M01234:01:000000000-AAAAA:1:1101:2020:0010 2:N:0:1
-GGCCTGTACCGTCAGCGTCATTGAGGCTGCGCCCATAGTGCTTCCTGCTGCTCCCAAGAATATCCCAAGGAACAAAGCTCCTATTCCCAC
+GGCCTGTACCGTCAGCGTCATTGACGCTGCGCCCATAGTGCTTCCTGCTGCTCCTAAGAATATCCCAAGGAACAGAGCTCCTATTCCCAC
 +
 AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
diff --git a/micall/tests/microtest/make_sample.py b/micall/tests/microtest/make_sample.py
index e4105deff..2604bc778 100644
--- a/micall/tests/microtest/make_sample.py
+++ b/micall/tests/microtest/make_sample.py
@@ -64,19 +64,19 @@ def main():
                    FastqFile('2020A-GP41_S4_L001_R1_001.fastq',
                              '2020',
                              False,
-                             (FastqSection('GP41',
-                                           2,
-                                           30,
+                             (FastqSection('HIV1-B-FR-KF716496-seed',
+                                           6957,
+                                           7044,
                                            10,
-                                           (CodonMutation(10, 'GGGATA'),)),)),
+                                           (CodonMutation(6981, 'GGGATA'),)),)),
                    FastqFile('2020A-GP41_S4_L001_R2_001.fastq',
                              '2020',
                              True,
-                             (FastqSection('GP41',
-                                           2,
-                                           30,
+                             (FastqSection('HIV1-B-FR-KF716496-seed',
+                                           6957,
+                                           7044,
                                            10,
-                                           (CodonMutation(10, 'GGGATA'),)),)),
+                                           (CodonMutation(6981, 'GGGATA'),)),)),
                    FastqFile('2040A-HLA-B_S6_L001_R1_001.fastq',
                              '2040',
                              False,
diff --git a/release_test_microtest.py b/release_test_microtest.py
index dd375b79e..cfdc10cba 100644
--- a/release_test_microtest.py
+++ b/release_test_microtest.py
@@ -119,7 +119,7 @@ def check_2020(self):
             pos = int(row['refseq.nuc.pos'])
             assert row['seed'] in expected_seeds, pos
             assert row['region'] == 'GP41', pos
-            if 3 < pos <= 81:
+            if 3 < pos <= 90:
                 assert row['coverage'] == '10', pos
             else:
                 assert row['coverage'] == '0', pos