diff --git a/src/Controls/src/Core/Handlers/Items/iOS/GridViewLayout.cs b/src/Controls/src/Core/Handlers/Items/iOS/GridViewLayout.cs
index 3330227909c2..bda227c7cc3e 100644
--- a/src/Controls/src/Core/Handlers/Items/iOS/GridViewLayout.cs
+++ b/src/Controls/src/Core/Handlers/Items/iOS/GridViewLayout.cs
@@ -243,7 +243,8 @@ bool NeedsSingleItemHorizontalAlignmentAdjustment(UICollectionViewLayoutAttribut
 				return false;
 			}
 
-			if (layoutAttributesForRectElements[0].Frame.Top != CollectionView.Frame.Top + CollectionView.ContentInset.Bottom)
+			// We need to determine whether this 'if' statement is needed, as its relevance is currently uncertain.
+			if (layoutAttributesForRectElements[0].Frame.Top != CollectionView.Frame.Top)
 			{
 				return false;
 			}
diff --git a/src/Controls/tests/TestCases.Android.Tests/snapshots/android/CollectionViewSingleItemAlignmentWithFooter.png b/src/Controls/tests/TestCases.Android.Tests/snapshots/android/CollectionViewSingleItemAlignmentWithFooter.png
new file mode 100644
index 000000000000..13b2bb2daf8d
Binary files /dev/null and b/src/Controls/tests/TestCases.Android.Tests/snapshots/android/CollectionViewSingleItemAlignmentWithFooter.png differ
diff --git a/src/Controls/tests/TestCases.HostApp/Issues/Issue21728.xaml b/src/Controls/tests/TestCases.HostApp/Issues/Issue21728.xaml
new file mode 100644
index 000000000000..09fac4d85cef
--- /dev/null
+++ b/src/Controls/tests/TestCases.HostApp/Issues/Issue21728.xaml
@@ -0,0 +1,22 @@
+<?xml version="1.0" encoding="utf-8" ?>
+<ContentPage xmlns="http://schemas.microsoft.com/dotnet/2021/maui"
+             xmlns:x="http://schemas.microsoft.com/winfx/2009/xaml"
+             x:Class="Maui.Controls.Sample.Issues.Issue21728"
+             xmlns:ns="clr-namespace:Maui.Controls.Sample.Issues">
+  <CollectionView x:Name="collectionview" AutomationId="collectionview" ItemsLayout="VerticalGrid, 2">
+		<CollectionView.ItemTemplate>
+			<DataTemplate>
+				<Label Text="{Binding Name}" BackgroundColor="Red" HeightRequest="200" HorizontalOptions="FillAndExpand" VerticalOptions="FillAndExpand"/>
+			</DataTemplate>
+		</CollectionView.ItemTemplate>
+		<CollectionView.Footer>
+			<ContentView >
+				<ContentView.ControlTemplate>
+					<ControlTemplate>
+						<Label Text="CollectionView Footer"/>
+					</ControlTemplate>
+				</ContentView.ControlTemplate>
+			</ContentView>
+		</CollectionView.Footer>
+	</CollectionView>
+</ContentPage>
\ No newline at end of file
diff --git a/src/Controls/tests/TestCases.HostApp/Issues/Issue21728.xaml.cs b/src/Controls/tests/TestCases.HostApp/Issues/Issue21728.xaml.cs
new file mode 100644
index 000000000000..da9a3efa0d7e
--- /dev/null
+++ b/src/Controls/tests/TestCases.HostApp/Issues/Issue21728.xaml.cs
@@ -0,0 +1,26 @@
+using System;
+using System.Collections.Generic;
+using System.Collections.ObjectModel;
+using Microsoft.Maui.Controls;
+
+namespace Maui.Controls.Sample.Issues;
+
+[Issue(IssueTracker.Github, 21728, "CollectionView item alignment issue when a single item is present with a footer", PlatformAffected.iOS)]
+public partial class Issue21728 : ContentPage
+{
+	public IList<TestItem> Items { get; set; }
+
+	public Issue21728()
+    {
+        InitializeComponent();
+		BindingContext = this;
+		Items = new List<TestItem>();
+		Items.Add(new TestItem() { Name = "Test Item 1" });
+		collectionview.ItemsSource = Items;
+	}
+	
+	public class TestItem
+	{
+		public string Name { get; set; }
+	}
+}
\ No newline at end of file
diff --git a/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue21728.cs b/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue21728.cs
new file mode 100644
index 000000000000..dcd86a00f205
--- /dev/null
+++ b/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue21728.cs
@@ -0,0 +1,26 @@
+#if !MACCATALYST
+using NUnit.Framework;
+using UITest.Appium;
+using UITest.Core;
+
+namespace Microsoft.Maui.TestCases.Tests.Issues
+{
+	public class Issue21728 : _IssuesUITest
+	{
+		public override string Issue => "CollectionView item alignment issue when a single item is present with a footer";
+
+		public Issue21728(TestDevice device)
+		: base(device)
+		{ }
+
+		[Test]
+		[Category(UITestCategories.CollectionView)]
+		public void CollectionViewSingleItemAlignmentWithFooter()
+		{
+			App.WaitForElement("collectionview");
+
+			VerifyScreenshot();
+		}
+	}
+}
+#endif
diff --git a/src/Controls/tests/TestCases.WinUI.Tests/snapshots/windows/CollectionViewSingleItemAlignmentWithFooter.png b/src/Controls/tests/TestCases.WinUI.Tests/snapshots/windows/CollectionViewSingleItemAlignmentWithFooter.png
new file mode 100644
index 000000000000..79f72661d3af
Binary files /dev/null and b/src/Controls/tests/TestCases.WinUI.Tests/snapshots/windows/CollectionViewSingleItemAlignmentWithFooter.png differ
diff --git a/src/Controls/tests/TestCases.iOS.Tests/snapshots/ios/CollectionViewSingleItemAlignmentWithFooter.png b/src/Controls/tests/TestCases.iOS.Tests/snapshots/ios/CollectionViewSingleItemAlignmentWithFooter.png
new file mode 100644
index 000000000000..20deb6211b8d
Binary files /dev/null and b/src/Controls/tests/TestCases.iOS.Tests/snapshots/ios/CollectionViewSingleItemAlignmentWithFooter.png differ