diff --git a/src/Controls/tests/TestCases.HostApp/Issues/Issue6387.cs b/src/Controls/tests/TestCases.HostApp/Issues/Issue6387.cs
new file mode 100644
index 000000000000..9f0028e9b813
--- /dev/null
+++ b/src/Controls/tests/TestCases.HostApp/Issues/Issue6387.cs
@@ -0,0 +1,23 @@
+namespace Controls.TestCases.HostApp.Issues;
+
+[Issue(IssueTracker.Github, 6387, "ArgumentException thrown when a negative value is set for the padding of a label", PlatformAffected.UWP)]
+public class Issue6387 : ContentPage
+{
+	public Issue6387()
+	{
+		Label labelWithNegativePaddingValue = new Label
+		{
+			AutomationId = "LabelWithNegativePaddingValue",
+			Text = "The test passes if the app runs without crashing and fails if the app crashes",
+			Padding = new Thickness(-2)
+		};
+
+		VerticalStackLayout verticalStackLayout = new VerticalStackLayout
+		{
+			Padding = 20,
+			Children = { labelWithNegativePaddingValue }
+		};
+
+		Content = verticalStackLayout;
+	}
+}
diff --git a/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue6387.cs b/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue6387.cs
new file mode 100644
index 000000000000..0c58626ac433
--- /dev/null
+++ b/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue6387.cs
@@ -0,0 +1,21 @@
+using NUnit.Framework;
+using UITest.Appium;
+using UITest.Core;
+
+namespace Microsoft.Maui.TestCases.Tests.Issues;
+
+public class Issue6387 : _IssuesUITest
+{
+	public Issue6387(TestDevice device) : base(device)
+	{
+	}
+
+	public override string Issue => "ArgumentException thrown when a negative value is set for the padding of a label";
+
+	[Test]
+	[Category(UITestCategories.Label)]
+	public void LabelWithNegativePaddingShouldNotThrowException()
+	{
+		App.WaitForElement("LabelWithNegativePaddingValue");
+	}
+}
\ No newline at end of file
diff --git a/src/Core/src/Platform/Windows/TextBlockExtensions.cs b/src/Core/src/Platform/Windows/TextBlockExtensions.cs
index 52f953006e75..90de45765f18 100644
--- a/src/Core/src/Platform/Windows/TextBlockExtensions.cs
+++ b/src/Core/src/Platform/Windows/TextBlockExtensions.cs
@@ -5,6 +5,7 @@
 using System.Xml.Linq;
 using System.Xml.Resolvers;
 using Microsoft.UI.Xaml.Controls;
+using WThickness = Microsoft.UI.Xaml.Thickness;
 using Microsoft.UI.Xaml.Documents;
 
 namespace Microsoft.Maui.Platform
@@ -31,8 +32,18 @@ public static void UpdateText(this TextBlock platformControl, ILabel label)
 		public static void UpdateTextColor(this TextBlock platformControl, ITextStyle text) =>
 			platformControl.UpdateProperty(TextBlock.ForegroundProperty, text.TextColor);
 
-		public static void UpdatePadding(this TextBlock platformControl, ILabel label) =>
-			platformControl.UpdateProperty(TextBlock.PaddingProperty, label.Padding.ToPlatform());
+		public static void UpdatePadding(this TextBlock platformControl, ILabel label)
+		{
+			// Label padding values do not support negative values; if specified, negative values will be replaced with zero
+			var padding = new WThickness(
+				Math.Max(0, label.Padding.Left),
+				Math.Max(0, label.Padding.Top),
+				Math.Max(0, label.Padding.Right),
+				Math.Max(0, label.Padding.Bottom)
+			);
+
+			platformControl.UpdateProperty(TextBlock.PaddingProperty, padding);
+		}
 
 		public static void UpdateCharacterSpacing(this TextBlock platformControl, ITextStyle label)
 		{