diff --git a/src/Controls/src/Core/Handlers/Items/CarouselViewHandler.Android.cs b/src/Controls/src/Core/Handlers/Items/CarouselViewHandler.Android.cs
index abe5e4b40480..6fa39583b485 100644
--- a/src/Controls/src/Core/Handlers/Items/CarouselViewHandler.Android.cs
+++ b/src/Controls/src/Core/Handlers/Items/CarouselViewHandler.Android.cs
@@ -95,7 +95,7 @@ double GetItemWidth()
 				if (double.IsInfinity(width))
 					return width;
 
-				itemWidth = (int)(width - Context?.ToPixels(VirtualView.PeekAreaInsets.Left) - Context?.ToPixels(VirtualView.PeekAreaInsets.Right) - Context?.ToPixels(listItemsLayout.ItemSpacing));
+				itemWidth = (int)(width - Context?.ToPixels(VirtualView.PeekAreaInsets.Left) - Context?.ToPixels(VirtualView.PeekAreaInsets.Right));
 			}
 
 			return itemWidth;
@@ -112,7 +112,7 @@ double GetItemHeight()
 				if (double.IsInfinity(height))
 					return height;
 
-				itemHeight = (int)(height - Context?.ToPixels(VirtualView.PeekAreaInsets.Top) - Context?.ToPixels(VirtualView.PeekAreaInsets.Bottom) - Context?.ToPixels(listItemsLayout.ItemSpacing));
+				itemHeight = (int)(height - Context?.ToPixels(VirtualView.PeekAreaInsets.Top) - Context?.ToPixels(VirtualView.PeekAreaInsets.Bottom));
 			}
 
 			return itemHeight;
diff --git a/src/Controls/tests/TestCases.Android.Tests/snapshots/android/CarouselViewShouldRenderCorrectly.png b/src/Controls/tests/TestCases.Android.Tests/snapshots/android/CarouselViewShouldRenderCorrectly.png
index bf3e23f5eedd..38d36f30bb96 100644
Binary files a/src/Controls/tests/TestCases.Android.Tests/snapshots/android/CarouselViewShouldRenderCorrectly.png and b/src/Controls/tests/TestCases.Android.Tests/snapshots/android/CarouselViewShouldRenderCorrectly.png differ
diff --git a/src/Controls/tests/TestCases.Android.Tests/snapshots/android/VerifySpacingAffectsItemSize.png b/src/Controls/tests/TestCases.Android.Tests/snapshots/android/VerifySpacingAffectsItemSize.png
new file mode 100644
index 000000000000..3939d43e4e45
Binary files /dev/null and b/src/Controls/tests/TestCases.Android.Tests/snapshots/android/VerifySpacingAffectsItemSize.png differ
diff --git a/src/Controls/tests/TestCases.HostApp/Issues/Issue29609.cs b/src/Controls/tests/TestCases.HostApp/Issues/Issue29609.cs
new file mode 100644
index 000000000000..7e4fade76612
--- /dev/null
+++ b/src/Controls/tests/TestCases.HostApp/Issues/Issue29609.cs
@@ -0,0 +1,72 @@
+using System.Collections.ObjectModel;
+
+namespace Maui.Controls.Sample.Issues;
+
+[Issue(IssueTracker.Github, 29609, "ItemSpacing on CarouselView resizes items", PlatformAffected.Android)]
+public class Issue29609 : ContentPage
+{
+    Issue29609_ViewModel ViewModel;
+    public Issue29609()
+    {
+        ViewModel = new Issue29609_ViewModel();
+        BindingContext = ViewModel;
+        var stack = new StackLayout
+        {
+            VerticalOptions = LayoutOptions.Center,
+            HorizontalOptions = LayoutOptions.Center
+        };
+        var label = new Label
+        {
+            Text = "ItemSpacing on CarouselView resizes items",
+            AutomationId = "29609DescriptionLabel"
+        };
+        var carouselView = new CarouselView
+        {
+            BackgroundColor = Colors.Red,
+            AutomationId = "29609CarouselView",
+            HeightRequest = 400,
+            WidthRequest = 300,
+            ItemsSource = ViewModel.Items,
+            Loop = false,
+            ItemsLayout = new LinearItemsLayout(ItemsLayoutOrientation.Horizontal)
+            {
+                ItemSpacing = 50,
+                SnapPointsAlignment = SnapPointsAlignment.Center,
+                SnapPointsType = SnapPointsType.MandatorySingle
+            },
+            ItemTemplate = new DataTemplate(() =>
+            {
+                var grid = new Grid
+                {
+                    BackgroundColor = Colors.Yellow
+                };
+
+                var label = new Label
+                {
+                    HorizontalOptions = LayoutOptions.Center,
+                    VerticalOptions = LayoutOptions.Center
+                };
+                label.SetBinding(Label.TextProperty, ".");
+
+                grid.Children.Add(label);
+                return grid;
+            })
+        };
+        stack.Children.Add(label);
+        stack.Children.Add(carouselView);
+        Content = stack;
+    }
+}
+
+public class Issue29609_ViewModel
+{
+    public ObservableCollection<string> Items = new();
+
+    public Issue29609_ViewModel()
+    {
+        for (var i = 0; i < 5; i++)
+        {
+            Items.Add($"Item {i}");
+        }
+    }
+}
\ No newline at end of file
diff --git a/src/Controls/tests/TestCases.Mac.Tests/snapshots/mac/VerifySpacingAffectsItemSize.png b/src/Controls/tests/TestCases.Mac.Tests/snapshots/mac/VerifySpacingAffectsItemSize.png
new file mode 100644
index 000000000000..582ec068f328
Binary files /dev/null and b/src/Controls/tests/TestCases.Mac.Tests/snapshots/mac/VerifySpacingAffectsItemSize.png differ
diff --git a/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue29609.cs b/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue29609.cs
new file mode 100644
index 000000000000..8b5d75f10f29
--- /dev/null
+++ b/src/Controls/tests/TestCases.Shared.Tests/Tests/Issues/Issue29609.cs
@@ -0,0 +1,24 @@
+# if TEST_FAILS_ON_WINDOWS // ItemSpacing on CarouselView is not applied on Windows https://github.com/dotnet/maui/issues/29772
+using NUnit.Framework;
+using UITest.Appium;
+using UITest.Core;
+
+namespace Microsoft.Maui.TestCases.Tests.Issues;
+
+public class Issue29609 : _IssuesUITest
+{
+    public Issue29609(TestDevice testDevice) : base(testDevice)
+    {
+    }
+
+    public override string Issue => "ItemSpacing on CarouselView resizes items";
+
+    [Test]
+    [Category(UITestCategories.CarouselView)]
+    public void VerifySpacingAffectsItemSize()
+    {
+        App.WaitForElement("29609DescriptionLabel");
+        VerifyScreenshot();
+    }
+}
+#endif
\ No newline at end of file
diff --git a/src/Controls/tests/TestCases.iOS.Tests/snapshots/ios/VerifySpacingAffectsItemSize.png b/src/Controls/tests/TestCases.iOS.Tests/snapshots/ios/VerifySpacingAffectsItemSize.png
new file mode 100644
index 000000000000..53557ac910b6
Binary files /dev/null and b/src/Controls/tests/TestCases.iOS.Tests/snapshots/ios/VerifySpacingAffectsItemSize.png differ