diff --git a/src/coreclr/tools/aot/crossgen2/crossgen2_publish.csproj b/src/coreclr/tools/aot/crossgen2/crossgen2_publish.csproj
index 70be01d7d14f1..948d29c14141e 100644
--- a/src/coreclr/tools/aot/crossgen2/crossgen2_publish.csproj
+++ b/src/coreclr/tools/aot/crossgen2/crossgen2_publish.csproj
@@ -10,6 +10,8 @@
     <!-- Can't use NativeAOT in source build yet https://github.com/dotnet/runtime/issues/66859 -->
     <NativeAotSupported Condition="'$(DotNetBuildFromSource)' == 'true'">false</NativeAotSupported>
     <NativeAotSupported Condition="$(OutputRID.StartsWith('tizen')) == 'true'">false</NativeAotSupported>
+    <!-- Publish crossgen2 as a single-file app on native-OS builds. Cross-OS NativeAOT compilation is not supported yet -->
+    <NativeAotSupported Condition="'$(CrossBuild)' == 'true' and '$(TargetOS)' != '$(HostOS)'">false</NativeAotSupported>
     <PublishTrimmed>true</PublishTrimmed>
     <RuntimeIdentifier>$(PackageRID)</RuntimeIdentifier>
     <SelfContained>true</SelfContained>
@@ -30,7 +32,7 @@
   </PropertyGroup>
 
   <Import Project="Sdk.targets" Sdk="Microsoft.NET.Sdk" />
-  
+
   <ItemGroup>
     <KnownFrameworkReference Remove="Microsoft.AspNetCore.App" />
     <KnownFrameworkReference Remove="Microsoft.WindowsDesktop.App" />