diff --git a/src/coreclr/nativeaot/Bootstrap/main.cpp b/src/coreclr/nativeaot/Bootstrap/main.cpp
index 4e73f89e3fd9..c713a1c1e08d 100644
--- a/src/coreclr/nativeaot/Bootstrap/main.cpp
+++ b/src/coreclr/nativeaot/Bootstrap/main.cpp
@@ -94,7 +94,6 @@ static char& __unbox_z = __stop___unbox;
 #endif // _MSC_VER
 
 extern "C" bool RhInitialize();
-extern "C" void RhSetRuntimeInitializationCallback(int (*fPtr)());
 
 extern "C" bool RhRegisterOSModule(void * pModule,
     void * pvManagedCodeStartRange, uint32_t cbManagedCodeRange,
@@ -169,10 +168,28 @@ extern "C" int __managed__Main(int argc, char* argv[]);
 #else
 #define NATIVEAOT_ENTRYPOINT __managed__Startup
 extern "C" void __managed__Startup();
+// _initialize is a function generated by the WASI SDK libc that calls the LLVM synthesized __wasm_call_ctors function for reactor components:
+// https://github.com/WebAssembly/wasi-libc/blob/9f51a7102085ec6a6ced5778f0864c9af9f50000/libc-bottom-half/crt/crt1-reactor.c#L7-L27
+// We define and call it for NATIVEAOT_DLL and TARGET_WASI to call all the global c++ static constructors.  This ensures the runtime is initialized
+// when calling into WebAssembly Component Model components
+#if defined(NATIVEAOT_DLL) && defined(TARGET_WASI)
+extern "C" void _initialize();
+
+// CustomNativeMain programs are built using the same libbootstrapperdll as NATIVEAOT_DLL but wasi-libc will not provide an _initialize implementation,
+// so create a dummy one here and make it weak to allow wasi-libc to provide the real implementation for WASI reactor components.
+__attribute__((weak)) void _initialize()
+{
+}
+
+#endif // TARGET_WASI
 #endif // !NATIVEAOT_DLL
 
 static int InitializeRuntime()
 {
+#if defined(NATIVEAOT_DLL) && defined(TARGET_WASI)
+    _initialize();
+#endif
+
     if (!RhInitialize())
         return -1;
 
@@ -200,6 +217,12 @@ static int InitializeRuntime()
     return 0;
 }
 
+#ifdef NATIVEAOT_DLL
+int (*g_RuntimeInitializationCallback)() = &InitializeRuntime;
+#else
+int (*g_RuntimeInitializationCallback)() = nullptr;
+#endif
+
 #ifndef NATIVEAOT_DLL
 
 #ifdef ENSURE_PRIMARY_STACK_SIZE
@@ -229,20 +252,3 @@ int main(int argc, char* argv[])
     return __managed__Main(argc, argv);
 }
 #endif // !NATIVEAOT_DLL
-
-#ifdef NATIVEAOT_DLL
-static struct InitializeRuntimePointerHelper
-{
-    InitializeRuntimePointerHelper()
-    {
-        RhSetRuntimeInitializationCallback(&InitializeRuntime);
-    }
-} initializeRuntimePointerHelper;
-
-extern "C" void* NativeAOT_StaticInitialization();
-
-void* NativeAOT_StaticInitialization()
-{
-    return &initializeRuntimePointerHelper;
-}
-#endif // NATIVEAOT_DLL
diff --git a/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.Unix.targets b/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.Unix.targets
index f88b9a2e0ac9..29d97fa8866c 100644
--- a/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.Unix.targets
+++ b/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.Unix.targets
@@ -142,7 +142,6 @@ The .NET Foundation licenses this file to you under the MIT license.
       <LinkerArg Include="-Wl,-z,relro" Condition="'$(_IsApplePlatform)' != 'true'" />
       <!-- binskim warning BA3011 The BIND_NOW flag is missing -->
       <LinkerArg Include="-Wl,-z,now" Condition="'$(_IsApplePlatform)' != 'true'" />
-      <LinkerArg Include="-Wl,-u,$(_SymbolPrefix)NativeAOT_StaticInitialization" Condition="'$(NativeLib)' == 'Shared' or '$(CustomNativeMain)' == 'true'" />
       <!-- this workaround can be deleted once the minimum supported glibc version
            (runtime's official build machine's glibc version) is at least 2.33
            see https://github.com/bminor/glibc/commit/99468ed45f5a58f584bab60364af937eb6f8afda -->
diff --git a/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.Windows.targets b/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.Windows.targets
index 9512bf826c40..8c912da082d9 100644
--- a/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.Windows.targets
+++ b/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.Windows.targets
@@ -87,7 +87,6 @@ The .NET Foundation licenses this file to you under the MIT license.
       <LinkerArg Include="/INCREMENTAL:NO" />
       <LinkerArg Condition="'$(LinkerSubsystem)' != ''" Include="/SUBSYSTEM:$(LinkerSubsystem)" />
       <LinkerArg Condition="'$(OutputType)' == 'WinExe' or '$(OutputType)' == 'Exe'" Include="/ENTRY:$(EntryPointSymbol) /NOEXP /NOIMPLIB" />
-      <LinkerArg Condition="'$(NativeLib)' == 'Shared' or '$(CustomNativeMain)' == 'true'" Include="/INCLUDE:NativeAOT_StaticInitialization" />
       <LinkerArg Include="/NATVIS:&quot;$(MSBuildThisFileDirectory)NativeAOT.natvis&quot;" />
       <LinkerArg Condition="'$(ControlFlowGuard)' == 'Guard'" Include="/guard:cf" />
     </ItemGroup>
diff --git a/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.targets b/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.targets
index 1f4bdbc95e52..389f7cef6408 100644
--- a/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.targets
+++ b/src/coreclr/nativeaot/BuildIntegration/Microsoft.NETCore.Native.targets
@@ -508,7 +508,6 @@ The .NET Foundation licenses this file to you under the MIT license.
       <CustomLinkerArg Include="-o &quot;$(NativeBinary.Replace(&quot;\&quot;, &quot;/&quot;))&quot;" />
       <CustomLinkerArg Condition="'$(NativeDebugSymbols)' == 'true'" Include="-g3" />
       <CustomLinkerArg Condition="'$(Optimize)' != 'true'" Include="$(WasmOptimizationSetting) -flto" />
-      <CustomLinkerArg Condition="$(NativeLib) == 'Shared' or '$(CustomNativeMain)' == 'true'" Include="-Wl,--export,NativeAOT_StaticInitialization" />
       <CustomLinkerArg Condition="'$(IlcLlvmTarget)' != ''" Include="-target $(IlcLlvmTarget)" />
     </ItemGroup>
 
@@ -523,7 +522,7 @@ The .NET Foundation licenses this file to you under the MIT license.
       <CustomLinkerArg Condition="'$(WasmEnableExceptionHandling)' == 'true'" Include="-fwasm-exceptions" />
     </ItemGroup>
 
-    <ItemGroup Condition  ="'$(_targetOS)' == 'wasi'" >
+    <ItemGroup Condition ="'$(_targetOS)' == 'wasi'" >
       <!-- Wasi has lots of undefined symbols currently, mostly globalization -->
       <CustomLinkerArg Include="-Wl,--unresolved-symbols=ignore-all -lstdc++ -Wl,--error-limit=0" />
       <CustomLinkerArg Include="--sysroot=&quot;$(WASI_SDK_PATH.Replace(&quot;\&quot;, &quot;/&quot;))/share/wasi-sysroot&quot;" />
diff --git a/src/coreclr/nativeaot/Runtime/thread.cpp b/src/coreclr/nativeaot/Runtime/thread.cpp
index 4b0ed2f9c0f5..f0e925ce5853 100644
--- a/src/coreclr/nativeaot/Runtime/thread.cpp
+++ b/src/coreclr/nativeaot/Runtime/thread.cpp
@@ -33,7 +33,7 @@ EXTERN_C NATIVEAOT_API void* REDHAWK_CALLCONV RhpHandleAlloc(void* pObject, int
 EXTERN_C NATIVEAOT_API void REDHAWK_CALLCONV RhHandleSet(void* handle, void* pObject);
 EXTERN_C NATIVEAOT_API void REDHAWK_CALLCONV RhHandleFree(void* handle);
 
-static int (*g_RuntimeInitializationCallback)();
+extern int (*g_RuntimeInitializationCallback)();
 static Thread* g_RuntimeInitializingThread;
 
 #endif //!DACCESS_COMPILE
@@ -1173,11 +1173,6 @@ FORCEINLINE bool Thread::InlineTryFastReversePInvoke(ReversePInvokeFrame * pFram
     return true;
 }
 
-EXTERN_C void RhSetRuntimeInitializationCallback(int (*fPtr)())
-{
-    g_RuntimeInitializationCallback = fPtr;
-}
-
 void Thread::ReversePInvokeAttachOrTrapThread(ReversePInvokeFrame * pFrame)
 {
     if (!IsStateSet(TSF_Attached))