diff --git a/.changeset/sweet-planes-begin.md b/.changeset/sweet-planes-begin.md
new file mode 100644
index 0000000000..8161c19534
--- /dev/null
+++ b/.changeset/sweet-planes-begin.md
@@ -0,0 +1,5 @@
+---
+"@emotion/jest": patch
+---
+
+Add `RegExp` as a valid type for the `target` option accepted by `toHaveStyleRule`.
diff --git a/packages/jest/src/matchers.js b/packages/jest/src/matchers.js
index 60fcf08781..f426c6d7eb 100644
--- a/packages/jest/src/matchers.js
+++ b/packages/jest/src/matchers.js
@@ -43,7 +43,7 @@ function toHaveStyleRule(
   received: *,
   property: *,
   value: *,
-  options?: { target?: string, media?: string } = {}
+  options?: { target?: string | RegExp, media?: string } = {}
 ) {
   const { target, media } = options
   const classNames = getClassNamesFromNodes([received])
diff --git a/packages/jest/types/index.d.ts b/packages/jest/types/index.d.ts
index f0b21ddb91..0eaab6bd41 100644
--- a/packages/jest/types/index.d.ts
+++ b/packages/jest/types/index.d.ts
@@ -9,7 +9,7 @@ type SnapshotSerializerPlugin = Extract<
 >
 
 export interface StyleRuleOptions {
-  target?: string
+  target?: string | RegExp
   media?: string
 }