diff --git a/packages/react-native/React/Base/RCTJSScriptLoaderModule.h b/packages/react-native/React/Base/RCTJSScriptLoaderModule.h
deleted file mode 100644
index f05d361a579411..00000000000000
--- a/packages/react-native/React/Base/RCTJSScriptLoaderModule.h
+++ /dev/null
@@ -1,22 +0,0 @@
-/*
- * Copyright (c) Meta Platforms, Inc. and affiliates.
- *
- * This source code is licensed under the MIT license found in the
- * LICENSE file in the root directory of this source tree.
- */
-
-@class RCTSource;
-
-NS_ASSUME_NONNULL_BEGIN
-
-/**
- * This protocol should be adopted when a turbo module needs to tell React Native to load a script.
- * In bridge-less React Native, it is a replacement for [_bridge loadAndExecuteSplitBundleURL:].
- */
-@protocol RCTJSScriptLoaderModule <NSObject>
-
-@property (nonatomic, copy) void (^loadScript)(RCTSource *source);
-
-@end
-
-NS_ASSUME_NONNULL_END
diff --git a/packages/react-native/ReactCommon/react/bridgeless/platform/ios/Core/RCTInstance.mm b/packages/react-native/ReactCommon/react/bridgeless/platform/ios/Core/RCTInstance.mm
index 872647242a43ba..a30f029e65f022 100644
--- a/packages/react-native/ReactCommon/react/bridgeless/platform/ios/Core/RCTInstance.mm
+++ b/packages/react-native/ReactCommon/react/bridgeless/platform/ios/Core/RCTInstance.mm
@@ -19,7 +19,6 @@
 #import <React/RCTDisplayLink.h>
 #import <React/RCTEventDispatcherProtocol.h>
 #import <React/RCTFollyConvert.h>
-#import <React/RCTJSScriptLoaderModule.h>
 #import <React/RCTJavaScriptLoader.h>
 #import <React/RCTLog.h>
 #import <React/RCTLogBox.h>
@@ -259,12 +258,6 @@ - (Class)getModuleClassFromName:(const char *)name
 - (void)_attachBridgelessAPIsToModule:(id<RCTTurboModule>)module FB_OBJC_DIRECT
 {
   __weak RCTInstance *weakInstance = self;
-  if ([module respondsToSelector:@selector(setLoadScript:)]) {
-    ((id<RCTJSScriptLoaderModule>)module).loadScript = ^(RCTSource *source) {
-      [weakInstance loadScriptFromSource:(source)];
-    };
-  }
-
   if ([module respondsToSelector:@selector(setDispatchToJSThread:)]) {
     ((id<RCTJSDispatcherModule>)module).dispatchToJSThread = ^(dispatch_block_t block) {
       __strong __typeof(self) strongSelf = weakInstance;