diff --git a/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.d.ts b/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.d.ts
index 6914853d2500f9..fea55884f5709c 100644
--- a/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.d.ts
+++ b/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.d.ts
@@ -17,6 +17,7 @@ type AccessibilityChangeEventName =
   | 'invertColorsChanged' // iOS-only Event
   | 'reduceMotionChanged'
   | 'highTextContrastChanged' // Android-only Event
+  | 'darkerSystemColorsChanged' // iOS-only Event
   | 'screenReaderChanged'
   | 'reduceTransparencyChanged'; // iOS-only Event
 
@@ -77,6 +78,13 @@ export interface AccessibilityInfoStatic {
    */
   isHighTextContrastEnabled: () => Promise<boolean>;
 
+  /**
+   * Query whether darker system colors is currently enabled.
+   *
+   * @platform ios
+   */
+  isDarkerSystemColorsEnabled: () => Promise<boolean>;
+
   /**
    * Query whether reduce motion and prefer cross-fade transitions settings are currently enabled.
    *
diff --git a/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.js b/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.js
index 755d0f3ed678e4..73940778356f57 100644
--- a/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.js
+++ b/packages/react-native/Libraries/Components/AccessibilityInfo/AccessibilityInfo.js
@@ -31,6 +31,7 @@ type AccessibilityEventDefinitionsIOS = {
   grayscaleChanged: [boolean],
   invertColorsChanged: [boolean],
   reduceTransparencyChanged: [boolean],
+  darkerSystemColorsChanged: [boolean],
 };
 
 type AccessibilityEventDefinitions = {
@@ -64,6 +65,7 @@ const EventNames: Map<
       ['reduceMotionChanged', 'reduceMotionChanged'],
       ['reduceTransparencyChanged', 'reduceTransparencyChanged'],
       ['screenReaderChanged', 'screenReaderChanged'],
+      ['darkerSystemColorsChanged', 'darkerSystemColorsChanged'],
     ]);
 
 /**
@@ -200,6 +202,32 @@ const AccessibilityInfo = {
     });
   },
 
+  /**
+   * Query whether dark system colors is currently enabled. iOS only.
+   *
+   * Returns a promise which resolves to a boolean.
+   * The result is `true` when dark system colors is enabled and `false` otherwise.
+   */
+  isDarkerSystemColorsEnabled(): Promise<boolean> {
+    return new Promise((resolve, reject) => {
+      if (Platform.OS === 'android') {
+        return Promise.resolve(false);
+      } else {
+        if (
+          NativeAccessibilityManagerIOS?.getCurrentDarkerSystemColorsState !=
+          null
+        ) {
+          NativeAccessibilityManagerIOS.getCurrentDarkerSystemColorsState(
+            resolve,
+            reject,
+          );
+        } else {
+          reject(null);
+        }
+      }
+    });
+  },
+
   /**
    * Query whether reduce motion and prefer cross-fade transitions settings are currently enabled.
    *
@@ -340,6 +368,9 @@ const AccessibilityInfo = {
    *     - `announcement`: The string announced by the screen reader.
    *     - `success`: A boolean indicating whether the announcement was
    *       successfully made.
+   * - `darkerSystemColorsChanged`: iOS-only event. Fires when the state of the dark system colors
+   *   toggle changes. The argument to the event handler is a boolean. The boolean is `true` when
+   *   dark system colors is enabled and `false` otherwise.
    *
    * These events are only supported on Android:
    *
diff --git a/packages/react-native/Libraries/__tests__/__snapshots__/public-api-test.js.snap b/packages/react-native/Libraries/__tests__/__snapshots__/public-api-test.js.snap
index 2bceffd5851558..15cd6c00cce1ac 100644
--- a/packages/react-native/Libraries/__tests__/__snapshots__/public-api-test.js.snap
+++ b/packages/react-native/Libraries/__tests__/__snapshots__/public-api-test.js.snap
@@ -1583,6 +1583,7 @@ type AccessibilityEventDefinitionsIOS = {
   grayscaleChanged: [boolean],
   invertColorsChanged: [boolean],
   reduceTransparencyChanged: [boolean],
+  darkerSystemColorsChanged: [boolean],
 };
 type AccessibilityEventDefinitions = {
   ...AccessibilityEventDefinitionsAndroid,
@@ -1598,6 +1599,7 @@ declare const AccessibilityInfo: {
   isInvertColorsEnabled(): Promise<boolean>,
   isReduceMotionEnabled(): Promise<boolean>,
   isHighTextContrastEnabled(): Promise<boolean>,
+  isDarkerSystemColorsEnabled(): Promise<boolean>,
   prefersCrossFadeTransitions(): Promise<boolean>,
   isReduceTransparencyEnabled(): Promise<boolean>,
   isScreenReaderEnabled(): Promise<boolean>,
diff --git a/packages/react-native/React/CoreModules/RCTAccessibilityManager.h b/packages/react-native/React/CoreModules/RCTAccessibilityManager.h
index 01af72e81420ff..e8ea831b931b0d 100644
--- a/packages/react-native/React/CoreModules/RCTAccessibilityManager.h
+++ b/packages/react-native/React/CoreModules/RCTAccessibilityManager.h
@@ -24,6 +24,7 @@ extern NSString *const RCTAccessibilityManagerDidUpdateMultiplierNotification; /
 @property (nonatomic, assign) BOOL isGrayscaleEnabled;
 @property (nonatomic, assign) BOOL isInvertColorsEnabled;
 @property (nonatomic, assign) BOOL isReduceMotionEnabled;
+@property (nonatomic, assign) BOOL isDarkerSystemColorsEnabled;
 @property (nonatomic, assign) BOOL prefersCrossFadeTransitions;
 @property (nonatomic, assign) BOOL isReduceTransparencyEnabled;
 @property (nonatomic, assign) BOOL isVoiceOverEnabled;
diff --git a/packages/react-native/React/CoreModules/RCTAccessibilityManager.mm b/packages/react-native/React/CoreModules/RCTAccessibilityManager.mm
index 4aaabcaba30be7..c32a12d57a776f 100644
--- a/packages/react-native/React/CoreModules/RCTAccessibilityManager.mm
+++ b/packages/react-native/React/CoreModules/RCTAccessibilityManager.mm
@@ -76,6 +76,11 @@ - (instancetype)init
                                                  name:UIAccessibilityReduceMotionStatusDidChangeNotification
                                                object:nil];
 
+    [[NSNotificationCenter defaultCenter] addObserver:self
+                                             selector:@selector(darkerSystemColorsDidChange:)
+                                                 name:UIAccessibilityDarkerSystemColorsStatusDidChangeNotification
+                                               object:nil];
+
     [[NSNotificationCenter defaultCenter] addObserver:self
                                              selector:@selector(reduceTransparencyStatusDidChange:)
                                                  name:UIAccessibilityReduceTransparencyStatusDidChangeNotification
@@ -91,6 +96,7 @@ - (instancetype)init
     _isGrayscaleEnabled = UIAccessibilityIsGrayscaleEnabled();
     _isInvertColorsEnabled = UIAccessibilityIsInvertColorsEnabled();
     _isReduceMotionEnabled = UIAccessibilityIsReduceMotionEnabled();
+    _isDarkerSystemColorsEnabled = UIAccessibilityDarkerSystemColorsEnabled();
     _isReduceTransparencyEnabled = UIAccessibilityIsReduceTransparencyEnabled();
     _isVoiceOverEnabled = UIAccessibilityIsVoiceOverRunning();
   }
@@ -169,6 +175,20 @@ - (void)reduceMotionStatusDidChange:(__unused NSNotification *)notification
   }
 }
 
+- (void)darkerSystemColorsDidChange:(__unused NSNotification *)notification
+{
+  BOOL newDarkerSystemColorsEnabled = UIAccessibilityDarkerSystemColorsEnabled();
+  if (_isDarkerSystemColorsEnabled != newDarkerSystemColorsEnabled) {
+    _isDarkerSystemColorsEnabled = newDarkerSystemColorsEnabled;
+#pragma clang diagnostic push
+#pragma clang diagnostic ignored "-Wdeprecated-declarations"
+    [[_moduleRegistry moduleForName:"EventDispatcher"] sendDeviceEventWithName:@"darkerSystemColorsChanged"
+                                                                          body:@(_isDarkerSystemColorsEnabled)];
+
+#pragma clang diagnostic pop
+  }
+}
+
 - (void)reduceTransparencyStatusDidChange:(__unused NSNotification *)notification
 {
   BOOL newReduceTransparencyEnabled = UIAccessibilityIsReduceTransparencyEnabled();
@@ -354,6 +374,13 @@ static void setMultipliers(
   onSuccess(@[ @(_isReduceMotionEnabled) ]);
 }
 
+RCT_EXPORT_METHOD(getCurrentDarkerSystemColorsState
+                  : (RCTResponseSenderBlock)onSuccess onError
+                  : (__unused RCTResponseSenderBlock)onError)
+{
+  onSuccess(@[ @(_isDarkerSystemColorsEnabled) ]);
+}
+
 RCT_EXPORT_METHOD(getCurrentPrefersCrossFadeTransitionsState
                   : (RCTResponseSenderBlock)onSuccess onError
                   : (__unused RCTResponseSenderBlock)onError)
diff --git a/packages/react-native/jest/setup.js b/packages/react-native/jest/setup.js
index c7f165b264173e..667100ed83e1f2 100644
--- a/packages/react-native/jest/setup.js
+++ b/packages/react-native/jest/setup.js
@@ -152,6 +152,7 @@ jest
       isInvertColorsEnabled: jest.fn(() => Promise.resolve(false)),
       isReduceMotionEnabled: jest.fn(() => Promise.resolve(false)),
       isHighTextContrastEnabled: jest.fn(() => Promise.resolve(false)),
+      isDarkerSystemColorsEnabled: jest.fn(() => Promise.resolve(false)),
       prefersCrossFadeTransitions: jest.fn(() => Promise.resolve(false)),
       isReduceTransparencyEnabled: jest.fn(() => Promise.resolve(false)),
       isScreenReaderEnabled: jest.fn(() => Promise.resolve(false)),
diff --git a/packages/react-native/src/private/specs/modules/NativeAccessibilityManager.js b/packages/react-native/src/private/specs/modules/NativeAccessibilityManager.js
index 20db1734da5866..6a978c3ea18560 100644
--- a/packages/react-native/src/private/specs/modules/NativeAccessibilityManager.js
+++ b/packages/react-native/src/private/specs/modules/NativeAccessibilityManager.js
@@ -29,6 +29,10 @@ export interface Spec extends TurboModule {
     onSuccess: (isReduceMotionEnabled: boolean) => void,
     onError: (error: Object) => void,
   ) => void;
+  +getCurrentDarkerSystemColorsState?: (
+    onSuccess: (isDarkerSystemColorsEnabled: boolean) => void,
+    onError: (error: Object) => void,
+  ) => void;
   +getCurrentPrefersCrossFadeTransitionsState?: (
     onSuccess: (prefersCrossFadeTransitions: boolean) => void,
     onError: (error: Object) => void,