diff --git a/tox.ini b/tox.ini
index 67d6166461..ef30e539b5 100644
--- a/tox.ini
+++ b/tox.ini
@@ -673,7 +673,9 @@ deps =
     strawberry-latest: strawberry-graphql[fastapi,flask]
 
     # Tornado
-    tornado: pytest<8.2
+    # Tornado <6.4.1 is incompatible with Pytest ≥8.2
+    # See https://github.com/tornadoweb/tornado/pull/3382.
+    tornado-{v6.0,v6.2}: pytest<8.2
     tornado-v6.0: tornado~=6.0.0
     tornado-v6.2: tornado~=6.2.0
     tornado-latest: tornado