diff --git a/CHANGELOG.md b/CHANGELOG.md
index 16a7edb01..f3d81d6f7 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -6,6 +6,10 @@
 
 - Add `getDefaultConfig` option to `getSentryExpoConfig` ([#3690](https://github.com/getsentry/sentry-react-native/pull/3690))
 
+### Fixes
+
+- Do not enable NativeFramesTracking when native is not available ([#3705](https://github.com/getsentry/sentry-react-native/pull/3705))
+
 ### Dependencies
 
 - Bump CLI from v2.30.0 to v2.30.2 ([#3678](https://github.com/getsentry/sentry-react-native/pull/3678))
diff --git a/samples/expo/app/_layout.tsx b/samples/expo/app/_layout.tsx
index e0cbc9c7d..6fac65744 100644
--- a/samples/expo/app/_layout.tsx
+++ b/samples/expo/app/_layout.tsx
@@ -54,7 +54,6 @@ process.env.EXPO_SKIP_DURING_EXPORT !== 'true' && Sentry.init({
       Sentry.metrics.metricsAggregatorIntegration(),
       new Sentry.ReactNativeTracing({
         routingInstrumentation,
-        enableNativeFramesTracking: !isExpoGo(), // Only in native builds, not in Expo Go.
       }),
     );
     return integrations.filter(i => i.name !== 'Dedupe');
diff --git a/src/js/tracing/reactnativetracing.ts b/src/js/tracing/reactnativetracing.ts
index e3c4a64cf..397cc1ee5 100644
--- a/src/js/tracing/reactnativetracing.ts
+++ b/src/js/tracing/reactnativetracing.ts
@@ -187,7 +187,10 @@ export class ReactNativeTracing implements Integration {
   /**
    *  Registers routing and request instrumentation.
    */
-  public setupOnce(addGlobalEventProcessor: (callback: EventProcessor) => void, getCurrentHub: () => Hub): void {
+  public async setupOnce(
+    addGlobalEventProcessor: (callback: EventProcessor) => void,
+    getCurrentHub: () => Hub,
+  ): Promise<void> {
     const hub = getCurrentHub();
     const client = hub.getClient();
     const clientOptions = client && client.getOptions();
@@ -203,7 +206,6 @@ export class ReactNativeTracing implements Integration {
       tracePropagationTargets: thisOptionsTracePropagationTargets,
       routingInstrumentation,
       enableAppStartTracking,
-      enableNativeFramesTracking,
       enableStallTracking,
     } = this.options;
 
@@ -240,20 +242,7 @@ export class ReactNativeTracing implements Integration {
       });
     }
 
-    if (enableNativeFramesTracking) {
-      NATIVE.enableNativeFramesTracking();
-      this.nativeFramesInstrumentation = new NativeFramesInstrumentation(addGlobalEventProcessor, () => {
-        const self = getCurrentHub().getIntegration(ReactNativeTracing);
-
-        if (self) {
-          return !!self.nativeFramesInstrumentation;
-        }
-
-        return false;
-      });
-    } else {
-      NATIVE.disableNativeFramesTracking();
-    }
+    this._enableNativeFramesTracking(addGlobalEventProcessor);
 
     if (enableStallTracking) {
       this.stallTrackingInstrumentation = new StallTrackingInstrumentation();
@@ -366,6 +355,40 @@ export class ReactNativeTracing implements Integration {
     return this._inflightInteractionTransaction;
   }
 
+  /**
+   * Enables or disables native frames tracking based on the `enableNativeFramesTracking` option.
+   */
+  private _enableNativeFramesTracking(addGlobalEventProcessor: (callback: EventProcessor) => void): void {
+    if (this.options.enableNativeFramesTracking && !NATIVE.enableNative) {
+      // Do not enable native frames tracking if native is not available.
+      logger.warn(
+        '[ReactNativeTracing] NativeFramesTracking is not available on the Web, Expo Go and other platforms without native modules.',
+      );
+      return;
+    }
+
+    if (!this.options.enableNativeFramesTracking && NATIVE.enableNative) {
+      // Disable native frames tracking when native available and option is false.
+      NATIVE.disableNativeFramesTracking();
+      return;
+    }
+
+    if (!this.options.enableNativeFramesTracking) {
+      return;
+    }
+
+    NATIVE.enableNativeFramesTracking();
+    this.nativeFramesInstrumentation = new NativeFramesInstrumentation(addGlobalEventProcessor, () => {
+      const self = getCurrentHub().getIntegration(ReactNativeTracing);
+
+      if (self) {
+        return !!self.nativeFramesInstrumentation;
+      }
+
+      return false;
+    });
+  }
+
   /**
    *  Sets the current view name into the app context.
    *  @param event Le event.