diff --git a/core/config/project_settings.cpp b/core/config/project_settings.cpp
index 75eea2ef5048..104b17961d6c 100644
--- a/core/config/project_settings.cpp
+++ b/core/config/project_settings.cpp
@@ -1528,6 +1528,7 @@ ProjectSettings::ProjectSettings() {
 
 	GLOBAL_DEF_RST("internationalization/rendering/force_right_to_left_layout_direction", false);
 	GLOBAL_DEF_BASIC(PropertyInfo(Variant::INT, "internationalization/rendering/root_node_layout_direction", PROPERTY_HINT_ENUM, "Based on Application Locale,Left-to-Right,Right-to-Left,Based on System Locale"), 0);
+	GLOBAL_DEF_BASIC("internationalization/rendering/root_node_auto_translate", true);
 
 	GLOBAL_DEF(PropertyInfo(Variant::INT, "gui/timers/incremental_search_max_interval_msec", PROPERTY_HINT_RANGE, "0,10000,1,or_greater"), 2000);
 
diff --git a/doc/classes/Node.xml b/doc/classes/Node.xml
index aea4082dbec2..37e64da8c8d3 100644
--- a/doc/classes/Node.xml
+++ b/doc/classes/Node.xml
@@ -970,8 +970,8 @@
 	</methods>
 	<members>
 		<member name="auto_translate_mode" type="int" setter="set_auto_translate_mode" getter="get_auto_translate_mode" enum="Node.AutoTranslateMode" default="0">
-			Defines if any text should automatically change to its translated version depending on the current locale (for nodes such as [Label], [RichTextLabel], [Window], etc.). See [enum AutoTranslateMode].
-			Also decides if the node's strings should be parsed for POT generation.
+			Defines if any text should automatically change to its translated version depending on the current locale (for nodes such as [Label], [RichTextLabel], [Window], etc.). Also decides if the node's strings should be parsed for POT generation.
+			[b]Note:[/b] For the root node, auto translate mode can also be set via [member ProjectSettings.internationalization/rendering/root_node_auto_translate].
 		</member>
 		<member name="editor_description" type="String" setter="set_editor_description" getter="get_editor_description" default="&quot;&quot;">
 			An optional description to the node. It will be displayed as a tooltip when hovering over the node in the editor's Scene dock.
diff --git a/doc/classes/ProjectSettings.xml b/doc/classes/ProjectSettings.xml
index a5aeee5bc4af..cfa8fbca2933 100644
--- a/doc/classes/ProjectSettings.xml
+++ b/doc/classes/ProjectSettings.xml
@@ -1436,6 +1436,10 @@
 		<member name="internationalization/rendering/force_right_to_left_layout_direction" type="bool" setter="" getter="" default="false">
 			Force layout direction and text writing direction to RTL for all controls.
 		</member>
+		<member name="internationalization/rendering/root_node_auto_translate" type="bool" setter="" getter="" default="true">
+			If [code]true[/code], root node will use [constant Node.AUTO_TRANSLATE_MODE_ALWAYS], otherwise [constant Node.AUTO_TRANSLATE_MODE_DISABLED] will be used.
+			[b]Note:[/b] This property is only read when the project starts. To change the auto translate mode at runtime, set [member Node.auto_translate_mode] of [member SceneTree.root] instead.
+		</member>
 		<member name="internationalization/rendering/root_node_layout_direction" type="int" setter="" getter="" default="0">
 			Root node default layout direction.
 		</member>
diff --git a/scene/main/scene_tree.cpp b/scene/main/scene_tree.cpp
index 04dd38fb5d2b..c465a3385fc5 100644
--- a/scene/main/scene_tree.cpp
+++ b/scene/main/scene_tree.cpp
@@ -1752,7 +1752,7 @@ SceneTree::SceneTree() {
 	root = memnew(Window);
 	root->set_min_size(Size2i(64, 64)); // Define a very small minimum window size to prevent bugs such as GH-37242.
 	root->set_process_mode(Node::PROCESS_MODE_PAUSABLE);
-	root->set_auto_translate_mode(Node::AUTO_TRANSLATE_MODE_ALWAYS);
+	root->set_auto_translate_mode(GLOBAL_GET("internationalization/rendering/root_node_auto_translate") ? Node::AUTO_TRANSLATE_MODE_ALWAYS : Node::AUTO_TRANSLATE_MODE_DISABLED);
 	root->set_name("root");
 	root->set_title(GLOBAL_GET("application/config/name"));