Starting QC [Time: 2018-06-28 22:18:42] [Mem usage: [84MB / 2024MB]] [Elapsed Time: 00:00:00.0000] QoRTs is Running in paired-end mode. QoRTs is Running in name-sorted mode. NOTE: Function "overlapMatch" requires function "mismatchEngine". Adding "mismatchEngine" to the active function list... Running functions: CigarOpDistribution, GCDistribution, GeneCalcs, InsertSize, JunctionCalcs, NVC, QualityScoreDistribution, StrandCheck, chromCounts, cigarLocusCounts, mismatchEngine, overlapMatch, readLengthDistro, writeBiotypeCounts, writeClippedNVC, writeDESeq, writeDEXSeq, writeGeneBody, writeGeneCounts, writeGenewiseGeneBody, writeJunctionSeqCounts, writeKnownSplices, writeNovelSplices, writeSpliceExon Checking first 10000 reads. Checking SAM file for formatting errors... [bam_sort_core] merging from 10 files... Exception in thread "main" java.lang.NoClassDefFoundError: net/sf/samtools/util/BlockGunzipper at net.sf.samtools.util.BlockCompressedInputStream.(BlockCompressedInputStream.java:60) at net.sf.samtools.util.BlockCompressedInputStream.(BlockCompressedInputStream.java:67) at net.sf.samtools.SAMFileReader.isBAMFile(SAMFileReader.java:690) at net.sf.samtools.SAMFileReader.init(SAMFileReader.java:640) at net.sf.samtools.SAMFileReader.(SAMFileReader.java:161) at net.sf.samtools.SAMFileReader.(SAMFileReader.java:131) at qcUtils.runAllQC$.runOnSeqFile(runAllQC.scala:1010) at qcUtils.runAllQC$.run(runAllQC.scala:960) at qcUtils.runAllQC$allQC_runner.run(runAllQC.scala:672) at runner.runner$.main(runner.scala:97) at runner.runner.main(runner.scala) Caused by: java.lang.ClassNotFoundException: net.sf.samtools.util.BlockGunzipper at java.net.URLClassLoader$1.run(URLClassLoader.java:370) at java.net.URLClassLoader$1.run(URLClassLoader.java:362) at java.security.AccessController.doPrivileged(Native Method) at java.net.URLClassLoader.findClass(URLClassLoader.java:361) at java.lang.ClassLoader.loadClass(ClassLoader.java:424) at sun.misc.Launcher$AppClassLoader.loadClass(Launcher.java:335) at java.lang.ClassLoader.loadClass(ClassLoader.java:357) ... 11 more Caused by: java.util.zip.ZipException: error reading zip file at java.util.zip.ZipFile.read(Native Method) at java.util.zip.ZipFile.access$1400(ZipFile.java:60) at java.util.zip.ZipFile$ZipFileInputStream.read(ZipFile.java:734) at java.util.zip.ZipFile$ZipFileInflaterInputStream.fill(ZipFile.java:434) at java.util.zip.InflaterInputStream.read(InflaterInputStream.java:158) at sun.misc.Resource.getBytes(Resource.java:124) at java.net.URLClassLoader.defineClass(URLClassLoader.java:462) at java.net.URLClassLoader.access$100(URLClassLoader.java:73) at java.net.URLClassLoader$1.run(URLClassLoader.java:368) ... 17 more