diff --git a/docs/getting_started/docker.md b/docs/getting_started/docker.md index 5de0f31b..0453ce24 100644 --- a/docs/getting_started/docker.md +++ b/docs/getting_started/docker.md @@ -10,20 +10,20 @@ Open your Windows Command Prompt by clicking the bottom left `Windows` button an Pull the container (this will take some time and storage space, but like an installation it only needs to be done once and can then be run on many datasets): - docker pull khanlab/hippunfold:v1.3.3 + docker pull khanlab/hippunfold:latest Run HippUnfold without any arguments to print the short help: - docker run -it --rm khanlab/hippunfold:v1.3.3 + docker run -it --rm khanlab/hippunfold:latest Use the `-h` option to get a detailed help listing: - docker run -it --rm khanlab/hippunfold:v1.3.3 -h + docker run -it --rm khanlab/hippunfold:latest -h Note that all the Snakemake command-line options are also available in HippUnfold, and can be listed with `--help-snakemake`: - docker run -it --rm khanlab/hippunfold:v1.3.3 --help-snakemake + docker run -it --rm khanlab/hippunfold:latest --help-snakemake ## Running an example @@ -47,13 +47,13 @@ ds002168/ Now let's run HippUnfold on the test dataset. Docker will need read/write access to the input and output directories, respectively. This is achieved with the `-v` flag. This 'binds' or 'mounts' a directory to a new directory inside the container. - docker run -it --rm -v c:\Users\jordan\Downloads\ds002168:/bids -v c:\Users\jordan\Downloads\ds002168_hippunfold:/output khanlab/hippunfold:v1.3.3 /bids /output participant --modality T1w -n + docker run -it --rm -v c:\Users\jordan\Downloads\ds002168:/bids -v c:\Users\jordan\Downloads\ds002168_hippunfold:/output khanlab/hippunfold:latest /bids /output participant --modality T1w -n Explanation: `-v c:\Users\jordan\Downloads\ds002168:/bids` tells Docker to mount the directory `c:\Users\jordan\Downloads\ds002168` into a new directory inside the container named `/bids`. We then do the same things for our output directory named `ds002168_hippunfold`, which we mount to `/output` inside the container. These arguments are not specific to HippUnfold but rather are general ways to use Docker. You may want to familiarize yourself with [Docker options](https://docs.docker.com/engine/reference/run/). -Everything after we specified the container (`khanlab/hippunfold:v1.3.3`) are arguments to HippUnfold itself. The first of these arguments (as with any BIDS App) are the input directory (`/bids`), the output directory (`/output`), and then the analysis level (`participant`). The `participant` analysis +Everything after we specified the container (`khanlab/hippunfold:latest`) are arguments to HippUnfold itself. The first of these arguments (as with any BIDS App) are the input directory (`/bids`), the output directory (`/output`), and then the analysis level (`participant`). The `participant` analysis level is used in HippUnfold for performing the segmentation, unfolding, and any participant-level processing. The `group` analysis is used to combine subfield volumes across subjects into a single tsv file. The `--modality` flag is also required, and describes which image we use for segmentation. Here we used the T1w image. We also used the `--dry-run/-n` option to just print out what would run, without actually running anything. @@ -68,7 +68,7 @@ useful if you are running multiple subjects. Running the following command (hippunfold on a single subject) may take ~30 minutes if you have 8 cores, shorter if you have more cores, but could be much longer (several hours) if you only have a single core. - docker run -it --rm -v c:\Users\jordan\Downloads\ds002168:/bids -v c:\Users\jordan\Downloads\ds002168_hippunfold:/output khanlab/hippunfold:v1.3.3 /bids /output participant --modality T1w -p --cores all + docker run -it --rm -v c:\Users\jordan\Downloads\ds002168:/bids -v c:\Users\jordan\Downloads\ds002168_hippunfold:/output khanlab/hippunfold:latest /bids /output participant --modality T1w -p --cores all After this completes, you should have a `ds002168_hippunfold` directory with outputs for the one subject. @@ -79,7 +79,7 @@ in the BIDS test dataset, you can use the `--modality T2w` option. In this case, test dataset has a limited FOV, we should also make use of the `--t1-reg-template` command-line option, which will make use of the T1w image for template registration, since a limited FOV T2w template does not exist. - docker run -it --rm -v c:\Users\jordan\Downloads\ds002168:/bids -v c:\Users\jordan\Downloads\ds002168_hippunfold_t2w:/output khanlab/hippunfold:v1.3.3 /bids /output participant --modality T2w --t1-reg-template -p --cores all + docker run -it --rm -v c:\Users\jordan\Downloads\ds002168:/bids -v c:\Users\jordan\Downloads\ds002168_hippunfold_t2w:/output khanlab/hippunfold:latest /bids /output participant --modality T2w --t1-reg-template -p --cores all Note that if you run with a different modality, you should use a separate output directory, since some of the files would be overwritten if not. diff --git a/docs/getting_started/singularity.md b/docs/getting_started/singularity.md index 4cb189fb..cfda2fd0 100644 --- a/docs/getting_started/singularity.md +++ b/docs/getting_started/singularity.md @@ -14,28 +14,23 @@ ## First time setup -Pull the container. This can be done from dockerhub, but this requires a large amount of disk space in your /tmp folder, since it has to convert from a docker container to a singularity container. To avoid this, we provide a Dropbox link to the singularity container itself: +Pull the container: - wget https://zenodo.org/record/8338481/files/hippunfold_v1.3.1.sif + singularity pull khanlab_hippunfold_latest.sif docker://khanlab/hippunfold:latest Run HippUnfold without any arguments to print the short help: - singularity run -e khanlab_hippunfold_v1.3.1.sif + singularity run -e khanlab_hippunfold_latest.sif Use the `-h` option to get a detailed help listing: - singularity run -e khanlab_hippunfold_v1.3.1.sif -h + singularity run -e khanlab_hippunfold_latest.sif -h Note that all the Snakemake command-line options are also available in HippUnfold, and can be listed with `--help-snakemake`: - singularity run -e khanlab_hippunfold_v1.3.1.sif --help-snakemake - -If you really need to pull the container from docker hub, you can use the following command, but beware, it is more prone to errors and will take up lots of system resources (e.g. ~70GB of free disk space): - - singularity pull khanlab_hippunfold_v1.3.1.sif docker://khanlab/hippunfold:v1.3.1 - + singularity run -e khanlab_hippunfold_latest.sif --help-snakemake Note: If you encounter any errors pulling the container from dockerhub, it may be because you are running out of disk space in your cache folders. Note, you can change these locations @@ -70,11 +65,11 @@ ds002168/ Now let's run HippUnfold. - singularity run -e khanlab_hippunfold_v1.3.1.sif ds002168 ds002168_hippunfold participant -n --modality T1w + singularity run -e khanlab_hippunfold_latest.sif ds002168 ds002168_hippunfold participant -n --modality T1w Explanation: -Everything prior to the container (`khanlab_hippunfold_v1.3.1.sif`) are arguments to singularity, and after are to HippUnfold itself. The first three arguments to HippUnfold (as with any BIDS App) are the input +Everything prior to the container (`khanlab_hippunfold_latest.sif`) are arguments to singularity, and after are to HippUnfold itself. The first three arguments to HippUnfold (as with any BIDS App) are the input folder (`ds002168`), the output folder (`ds002168_hippunfold`), and then the analysis level (`participant`). The `participant` analysis level is used in HippUnfold for performing the segmentation, unfolding, and any participant-level processing. The `group` analysis is used to combine subfield volumes @@ -88,7 +83,7 @@ When you run the above command, a long listing will print out, describing all th will be run. This is a long listing, and you can better appreciate it with the `less` tool. We can also have the shell command used for each rule printed to screen using the `-p` Snakemake option: - singularity run -e khanlab_hippunfold_v1.3.1.sif ds002168 ds002168_hippunfold participant -np --modality T1w | less + singularity run -e khanlab_hippunfold_latest.sif ds002168 ds002168_hippunfold participant -np --modality T1w | less Now, to actually run the workflow, we need to specify how many cores to use and leave out @@ -101,7 +96,7 @@ Running the following command (hippunfold on a single subject) may take ~30 minu cores, but could be much longer (several hours) if you only have a single core. - singularity run -e khanlab_hippunfold_v1.3.1.sif ds002168 ds002168_hippunfold participant -p --cores all --modality T1w + singularity run -e khanlab_hippunfold_latest.sif ds002168 ds002168_hippunfold participant -p --cores all --modality T1w Note that you may need to adjust your [Singularity options](https://sylabs.io/guides/3.1/user-guide/cli/singularity_run.html) to ensure the container can read and write to yout input and output directories, respectively. You can bind paths easily by setting an @@ -120,7 +115,7 @@ in the BIDS test dataset, you can use the `--modality T2w` option. In this case, test dataset has a limited FOV, we should also make use of the `--t1-reg-template` command-line option, which will make use of the T1w image for template registration, since a limited FOV T2w template does not exist. - singularity run -e khanlab_hippunfold_v1.3.1.sif ds002168 ds002168_hippunfold_t2w participant --modality T2w --t1-reg-template -p --cores all + singularity run -e khanlab_hippunfold_latest.sif ds002168 ds002168_hippunfold_t2w participant --modality T2w --t1-reg-template -p --cores all Note that if you run with a different modality, you should use a separate output folder, since some of the files would be overwritten if not. diff --git a/docs/getting_started/vagrant.md b/docs/getting_started/vagrant.md index bcee5e98..3ed7fba5 100644 --- a/docs/getting_started/vagrant.md +++ b/docs/getting_started/vagrant.md @@ -67,22 +67,9 @@ speed: 2 ## Download the HippUnfold container -We download the Singularity container for HippUnfold using -Dropbox in this example: +We pull/build the container from DockerHub: ``` -wget https://www.dropbox.com/s/jtf6zyy0u8sc2k6/khanlab_hippunfold_v1.2.0.sif -``` - -Note, you can also pull/build the container from DockerHub: -``` -singularity pull docker://khanlab/hippunfold:v1.2.0 -``` - -```{asciinema} ../casts/vagrant_hippunfold_get_app_dropbox.cast ---- -preload: 1 -speed: 2 ---- +singularity pull docker://khanlab/hippunfold:latest ``` ## Run HippUnfold