diff --git a/src/Illuminate/Foundation/Configuration/Middleware.php b/src/Illuminate/Foundation/Configuration/Middleware.php
index e8916d552d44..cf58263d830a 100644
--- a/src/Illuminate/Foundation/Configuration/Middleware.php
+++ b/src/Illuminate/Foundation/Configuration/Middleware.php
@@ -608,15 +608,15 @@ public function trimStrings(array $except = [])
     /**
      * Indicate that the trusted host middleware should be enabled.
      *
-     * @param  array<int, string>|null  $at
+     * @param  array<int, string>|(callable(): array<int, string>)|null  $at
      * @param  bool  $subdomains
      * @return $this
      */
-    public function trustHosts(array $at = null, bool $subdomains = true)
+    public function trustHosts(array|callable $at = null, bool $subdomains = true)
     {
         $this->trustHosts = true;
 
-        if (is_array($at)) {
+        if (! is_null($at)) {
             TrustHosts::at($at, $subdomains);
         }
 
diff --git a/src/Illuminate/Http/Middleware/TrustHosts.php b/src/Illuminate/Http/Middleware/TrustHosts.php
index 13dd1f00b62c..8eae16d1cec0 100644
--- a/src/Illuminate/Http/Middleware/TrustHosts.php
+++ b/src/Illuminate/Http/Middleware/TrustHosts.php
@@ -17,10 +17,17 @@ class TrustHosts
     /**
      * The trusted hosts that have been configured to always be trusted.
      *
-     * @var array<int, string>|null
+     * @var array<int, string>|(callable(): array<int, string>)|null
      */
     protected static $alwaysTrust;
 
+    /**
+     * Indicates whether subdomains of the application URL should be trusted.
+     *
+     * @var bool|null
+     */
+    protected static $subdomains;
+
     /**
      * Create a new middleware instance.
      *
@@ -39,9 +46,21 @@ public function __construct(Application $app)
      */
     public function hosts()
     {
-        return is_array(static::$alwaysTrust)
-            ? static::$alwaysTrust
-            : [$this->allSubdomainsOfApplicationUrl()];
+        if (is_null(static::$alwaysTrust)) {
+            return [$this->allSubdomainsOfApplicationUrl()];
+        }
+
+        $hosts = match (true) {
+            is_array(static::$alwaysTrust) => static::$alwaysTrust,
+            is_callable(static::$alwaysTrust) => call_user_func(static::$alwaysTrust),
+            default => [],
+        };
+
+        if (static::$subdomains) {
+            $hosts[] = $this->allSubdomainsOfApplicationUrl();
+        }
+
+        return $hosts;
     }
 
     /**
@@ -63,19 +82,14 @@ public function handle(Request $request, $next)
     /**
      * Specify the hosts that should always be trusted.
      *
-     * @param  array<int, string>  $hosts
+     * @param  array<int, string>|(callable(): array<int, string>)  $hosts
      * @param  bool  $subdomains
      * @return void
      */
-    public static function at(array $hosts, bool $subdomains = true)
+    public static function at(array|callable $hosts, bool $subdomains = true)
     {
-        if ($subdomains) {
-            if ($host = parse_url(config('app.url'), PHP_URL_HOST)) {
-                $hosts[] = '^(.+\.)?'.preg_quote($host).'$';
-            }
-        }
-
         static::$alwaysTrust = $hosts;
+        static::$subdomains = $subdomains;
     }
 
     /**
@@ -109,5 +123,6 @@ protected function allSubdomainsOfApplicationUrl()
     public static function flushState()
     {
         static::$alwaysTrust = null;
+        static::$subdomains = null;
     }
 }
diff --git a/tests/Foundation/Configuration/MiddlewareTest.php b/tests/Foundation/Configuration/MiddlewareTest.php
index 9012b4d5fa0c..8fde56c7a9c1 100644
--- a/tests/Foundation/Configuration/MiddlewareTest.php
+++ b/tests/Foundation/Configuration/MiddlewareTest.php
@@ -2,7 +2,6 @@
 
 namespace Illuminate\Tests\Foundation\Configuration;
 
-use Illuminate\Config\Repository;
 use Illuminate\Container\Container;
 use Illuminate\Contracts\Encryption\Encrypter;
 use Illuminate\Contracts\Foundation\Application;
@@ -219,23 +218,29 @@ protected function allSubdomainsOfApplicationUrl()
         $configuration->trustHosts();
         $this->assertEquals(['^(.+\.)?laravel\.test$'], $middleware->hosts());
 
-        app()['config'] = Mockery::mock(Repository::class);
-        app()['config']->shouldReceive('get')->with('app.url', null)->times(3)->andReturn('http://laravel.test');
-
         $configuration->trustHosts(at: ['my.test']);
         $this->assertEquals(['my.test', '^(.+\.)?laravel\.test$'], $middleware->hosts());
 
-        $configuration->trustHosts(at: ['my.test']);
+        $configuration->trustHosts(at: static fn () => ['my.test']);
         $this->assertEquals(['my.test', '^(.+\.)?laravel\.test$'], $middleware->hosts());
 
         $configuration->trustHosts(at: ['my.test'], subdomains: false);
         $this->assertEquals(['my.test'], $middleware->hosts());
 
+        $configuration->trustHosts(at: static fn () => ['my.test'], subdomains: false);
+        $this->assertEquals(['my.test'], $middleware->hosts());
+
         $configuration->trustHosts(at: []);
         $this->assertEquals(['^(.+\.)?laravel\.test$'], $middleware->hosts());
 
+        $configuration->trustHosts(at: static fn () => []);
+        $this->assertEquals(['^(.+\.)?laravel\.test$'], $middleware->hosts());
+
         $configuration->trustHosts(at: [], subdomains: false);
         $this->assertEquals([], $middleware->hosts());
+
+        $configuration->trustHosts(at: static fn () => [], subdomains: false);
+        $this->assertEquals([], $middleware->hosts());
     }
 
     public function testEncryptCookies()