diff --git a/src/Illuminate/Bus/DatabaseBatchRepository.php b/src/Illuminate/Bus/DatabaseBatchRepository.php
index d6130ede6c63..2e0c7c68a9e6 100644
--- a/src/Illuminate/Bus/DatabaseBatchRepository.php
+++ b/src/Illuminate/Bus/DatabaseBatchRepository.php
@@ -319,7 +319,7 @@ public function transaction(Closure $callback)
      */
     public function rollBack()
     {
-        $this->connection->rollBack();
+        $this->connection->rollBack(toLevel: 0);
     }
 
     /**
diff --git a/src/Illuminate/Queue/Jobs/Job.php b/src/Illuminate/Queue/Jobs/Job.php
index a41b401dddb7..8c5c7c76279c 100755
--- a/src/Illuminate/Queue/Jobs/Job.php
+++ b/src/Illuminate/Queue/Jobs/Job.php
@@ -204,6 +204,12 @@ public function fail($e = null)
             }
         }
 
+        if ($this->shouldRollBackDatabaseTransaction($e)) {
+            $this->container->make('db')
+                ->connection($this->container['config']['queue.failed.database'])
+                ->rollBack(toLevel: 0);
+        }
+
         try {
             // If the job has failed, we will delete it, call the "failed" method and then call
             // an event indicating the job has failed so it can be logged if needed. This is
@@ -218,6 +224,20 @@ public function fail($e = null)
         }
     }
 
+    /**
+     * Determine if the current database transaction should be rolled back to level zero.
+     *
+     * @param  \Throwable  $e
+     * @return bool
+     */
+    protected function shouldRollBackDatabaseTransaction($e)
+    {
+        return ($e instanceof TimeoutExceededException &&
+            $this->container['config']['queue.failed.database'] &&
+            in_array($this->container['config']['queue.failed.driver'], ['database', 'database-uuids']) &&
+            $this->container->bound('db'));
+    }
+
     /**
      * Process an exception that caused the job to fail.
      *
diff --git a/tests/Integration/Database/Queue/Fixtures/TimeOutJobWithNestedTransactions.php b/tests/Integration/Database/Queue/Fixtures/TimeOutJobWithNestedTransactions.php
new file mode 100644
index 000000000000..821b7bcac34d
--- /dev/null
+++ b/tests/Integration/Database/Queue/Fixtures/TimeOutJobWithNestedTransactions.php
@@ -0,0 +1,24 @@
+<?php
+
+namespace Illuminate\Tests\Integration\Database\Queue\Fixtures;
+
+use Illuminate\Bus\Batchable;
+use Illuminate\Bus\Queueable;
+use Illuminate\Contracts\Queue\ShouldQueue;
+use Illuminate\Queue\InteractsWithQueue;
+use Illuminate\Support\Facades\DB;
+
+class TimeOutJobWithNestedTransactions implements ShouldQueue
+{
+    use InteractsWithQueue, Queueable, Batchable;
+
+    public int $tries = 1;
+    public int $timeout = 2;
+
+    public function handle(): void
+    {
+        DB::transaction(function () {
+            DB::transaction(fn () => sleep(20));
+        });
+    }
+}
diff --git a/tests/Integration/Database/Queue/Fixtures/TimeOutNonBatchableJobWithNestedTransactions.php b/tests/Integration/Database/Queue/Fixtures/TimeOutNonBatchableJobWithNestedTransactions.php
new file mode 100644
index 000000000000..1d383e90b8bb
--- /dev/null
+++ b/tests/Integration/Database/Queue/Fixtures/TimeOutNonBatchableJobWithNestedTransactions.php
@@ -0,0 +1,23 @@
+<?php
+
+namespace Illuminate\Tests\Integration\Database\Queue\Fixtures;
+
+use Illuminate\Bus\Queueable;
+use Illuminate\Contracts\Queue\ShouldQueue;
+use Illuminate\Queue\InteractsWithQueue;
+use Illuminate\Support\Facades\DB;
+
+class TimeOutNonBatchableJobWithNestedTransactions implements ShouldQueue
+{
+    use InteractsWithQueue, Queueable;
+
+    public int $tries = 1;
+    public int $timeout = 2;
+
+    public function handle(): void
+    {
+        DB::transaction(function () {
+            DB::transaction(fn () => sleep(20));
+        });
+    }
+}
diff --git a/tests/Integration/Database/Queue/Fixtures/TimeOutNonBatchableJobWithTransaction.php b/tests/Integration/Database/Queue/Fixtures/TimeOutNonBatchableJobWithTransaction.php
new file mode 100644
index 000000000000..494e9fbdd704
--- /dev/null
+++ b/tests/Integration/Database/Queue/Fixtures/TimeOutNonBatchableJobWithTransaction.php
@@ -0,0 +1,21 @@
+<?php
+
+namespace Illuminate\Tests\Integration\Database\Queue\Fixtures;
+
+use Illuminate\Bus\Queueable;
+use Illuminate\Contracts\Queue\ShouldQueue;
+use Illuminate\Queue\InteractsWithQueue;
+use Illuminate\Support\Facades\DB;
+
+class TimeOutNonBatchableJobWithTransaction implements ShouldQueue
+{
+    use InteractsWithQueue, Queueable;
+
+    public int $tries = 1;
+    public int $timeout = 2;
+
+    public function handle(): void
+    {
+        DB::transaction(fn () => sleep(20));
+    }
+}
diff --git a/tests/Integration/Database/Queue/QueueTransactionTest.php b/tests/Integration/Database/Queue/QueueTransactionTest.php
index 9bfa3ca87a64..eac0c3fc452d 100644
--- a/tests/Integration/Database/Queue/QueueTransactionTest.php
+++ b/tests/Integration/Database/Queue/QueueTransactionTest.php
@@ -7,6 +7,7 @@
 use Illuminate\Tests\Integration\Database\DatabaseTestCase;
 use Orchestra\Testbench\Attributes\WithConfig;
 use Orchestra\Testbench\Attributes\WithMigration;
+use PHPUnit\Framework\Attributes\DataProvider;
 use PHPUnit\Framework\Attributes\RequiresPhpExtension;
 use Symfony\Component\Process\Exception\ProcessSignaledException;
 use Throwable;
@@ -29,9 +30,10 @@ protected function setUp(): void
         }
     }
 
-    public function testItCanHandleTimeoutJob()
+    #[DataProvider('timeoutJobs')]
+    public function testItCanHandleTimeoutJob($job)
     {
-        dispatch(new Fixtures\TimeOutJobWithTransaction);
+        dispatch($job);
 
         $this->assertSame(1, DB::table('jobs')->count());
         $this->assertSame(0, DB::table('failed_jobs')->count());
@@ -49,4 +51,14 @@ public function testItCanHandleTimeoutJob()
         $this->assertSame(0, DB::table('jobs')->count());
         $this->assertSame(1, DB::table('failed_jobs')->count());
     }
+
+    public static function timeoutJobs(): array
+    {
+        return [
+            [new Fixtures\TimeOutJobWithTransaction()],
+            [new Fixtures\TimeOutJobWithNestedTransactions()],
+            [new Fixtures\TimeOutNonBatchableJobWithTransaction()],
+            [new Fixtures\TimeOutNonBatchableJobWithNestedTransactions()],
+        ];
+    }
 }