diff --git a/MACS3/Commands/hmmratac_cmd.py b/MACS3/Commands/hmmratac_cmd.py index 54632293..d897e62e 100644 --- a/MACS3/Commands/hmmratac_cmd.py +++ b/MACS3/Commands/hmmratac_cmd.py @@ -1,4 +1,4 @@ -# Time-stamp: <2023-07-28 12:10:12 Tao Liu> +# Time-stamp: <2023-08-02 17:32:47 Tao Liu> """Description: Main HMMR command @@ -26,7 +26,7 @@ from MACS3.Utilities.OptValidator import opt_validate_hmmratac from MACS3.IO.PeakIO import PeakIO from MACS3.IO.PeakIO import BroadPeakIO -from MACS3.IO.Parser import BAMPEParser #BAMaccessor +from MACS3.IO.Parser import BAMPEParser, BEDPEParser #BAMaccessor from MACS3.Signal.HMMR_EM import HMMR_EM from MACS3.Signal.HMMR_Signal_Processing import generate_weight_mapping, generate_digested_signals, extract_signals_from_regions from MACS3.Signal.HMMR_HMM import hmm_training, hmm_predict, hmm_model_init, hmm_model_save @@ -71,20 +71,26 @@ def run( args ): cutoffanalysis_file = os.path.join( options.outdir, options.name+"_cutoff_analysis.tsv" ) ############################################# - # 1. Read the input BAM files + # 1. Read the input files ############################################# options.info("\n" + options.argtxt) - #options.info("Some important parameters for this run") - #options.info(f" binsize for HMM: {options.binsize} ()") - options.info("#1 Read fragments from BAM file...") - bam = BAMPEParser(options.bam_file[0], buffer_size=options.buffer_size) - petrack = bam.build_petrack() - if len( options.bam_file ) > 1: + if options.format == "BAMPE": + options.info("#1 Read fragments from BAMPE file...") + parser = BAMPEParser + elif options.format == "BEDPE": + options.info("#1 Read fragments from BEDPE file...") + parser = BEDPEParser + else: + raise Exception("wrong format") + + alignment = parser(options.input_file[0], buffer_size=options.buffer_size) + petrack = alignment.build_petrack() + if len( options.input_file ) > 1: # multiple input - for bamfile in options.bam_file[1:]: - bam = BAMPEParser(bamfile, buffer_size=options.buffer_size) - petrack = bam.append_petrack( petrack ) + for inputfile in options.input_file[1:]: + alignment = parser(inputfile, buffer_size=options.buffer_size) + petrack = alignment.append_petrack( petrack ) # remember to finalize the petrack petrack.finalize() diff --git a/bin/macs3 b/bin/macs3 index 3b52a526..dc54d529 100644 --- a/bin/macs3 +++ b/bin/macs3 @@ -1,5 +1,5 @@ #!/usr/bin/env python -# Time-stamp: <2023-07-28 12:14:37 Tao Liu> +# Time-stamp: <2023-08-02 17:49:12 Tao Liu> """Description: MACS v3 main executable. @@ -759,7 +759,19 @@ around open chromatin regions. Here's an example of how to run the `hmmratac` command: ``` -$ macs3 hmmratac -b test/yeast_500k_SRR1822137.bam -n hmmratac_yeast500k +$ macs3 hmmratac -i yeast.bam -n yeast +``` + +or with the BEDPE format + +``` +$ macs3 hmmratac -i yeast.bedpe.gz -f BEDPE -n yeast +``` + +Note: you can convert BAMPE to BEDPE by using + +``` +$ macs3 filterdup --keep-dup all -f BAMPE -i yeast.bam -o yeast.bedpe ``` Before proceeding, it's essential to carefully read the help messages @@ -774,11 +786,11 @@ crucial parameters we usually need to specify: These three parameters can significantly influence the results. Therefore, it's highly recommended to run `macs3 hmmratac ---cutoff-analysis-only -b your.bam` first, which will help you decide +--cutoff-analysis-only -i your.bam` first, which will help you decide the parameters for `-l`, `-u`, and `-c`. Since there isn't an automatic way to determine these parameters, your judgement will be vital. Please read the output from `macs3 hmmratac ---cutoff-analysis-only -b your.bam` and the following section `Choices +--cutoff-analysis-only -i your.bam` and the following section `Choices of cutoff values` for guidance. * Choices of cutoff values * @@ -877,8 +889,12 @@ plus an extra option for the HMM model file like `macs3 hmmratac # group for input files group_input = argparser_hmmratac.add_argument_group( "Input files arguments" ) - group_input.add_argument( "-b", "--bam", dest = "bam_file", type = str, required = True, nargs = "+", - help = "BAM files containing the aligment results for ATAC-seq paired end reads. If multiple files are given as '-t A B C', then they will all be read and pooled together. The file should be in BAMPE format (aligned in paired end mode). REQUIRED." ) + group_input.add_argument( "-i", "--input", dest = "input_file", type = str, required = True, nargs = "+", + help = "Input files containing the aligment results for ATAC-seq paired end reads. If multiple files are given as '-t A B C', then they will all be read and pooled together. The file should be in BAMPE or BEDPE format (aligned in paired end mode). Files can be gzipped. Note: all files should be in the same format! REQUIRED." ) + group_input.add_argument( "-f", "--format", dest = "format", type = str, + choices = ("BAMPE", "BEDPE"), + help = "Format of input files, \"BAMPE\" or \"BEDPE\". If there are multiple files, they should be in the same format -- either BAMPE or BEDPE. Please check the definition in README. Also please note that the BEDPE only contains three columns -- chromosome, left position of the whole pair, right position of the whole pair-- and is NOT the same BEDPE format used by BEDTOOLS. To convert BAMPE to BEDPE, you can use this command `macs3 filterdup --keep-dup all -f BAMPE -i input.bam -o output.bedpe`. DEFAULT: \"BAMPE\"", + default = "BAMPE" ) # group for output files group_output = argparser_hmmratac.add_argument_group( "Output arguments" ) diff --git a/test/cmdlinetest b/test/cmdlinetest index 2eaa4e15..6788ab24 100755 --- a/test/cmdlinetest +++ b/test/cmdlinetest @@ -1,5 +1,5 @@ #!/bin/bash -# Time-stamp: <2022-06-06 12:13:43 Tao Liu> +# Time-stamp: <2023-08-02 17:59:43 Tao Liu> # integrative subcmds testing @@ -38,6 +38,7 @@ CTRLBIGSPEEDTEST=input_12878_5M.bed.gz CALLVARPEAK=callvar_testing.narrowPeak ATACSEQBAM=yeast_500k_SRR1822137.bam +ATACSEQBED=yeast_500k_SRR1822137.bedpe.gz # callpeak echo "1. callpeak" @@ -211,16 +212,18 @@ macs3 randsample -i $CHIPCONTIGS50K -n 100000 --seed 31415926 --outdir ${OUTPUTD echo "15. hmmratac save training regions bed file and model file" # model file is written by default mkdir ${OUTPUTDIR_PREFIX}_run_hmmratac -macs3 hmmratac -b $ATACSEQBAM -n hmmratac_yeast500k --save-training-data --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log +macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k --save-training-data --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac.log + +macs3 hmmratac -i $ATACSEQBED -n hmmratac_yeast500k_bedpe -f BEDPE --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_bedpe.log TRAINING_REGIONS_BED=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_training_regions.bed HMM_MODEL=${OUTPUTDIR_PREFIX}_run_hmmratac/hmmratac_yeast500k_model.json echo "15.1 hmmratac load training regions from bedfile" -macs3 hmmratac -b $ATACSEQBAM -n hmmratac_yeast500k_load_training_regions -t ${TRAINING_REGIONS_BED} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions.log +macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_training_regions -t ${TRAINING_REGIONS_BED} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_training_regions.log echo "15.2 hmmratac load hmm model file" -macs3 hmmratac -b $ATACSEQBAM -n hmmratac_yeast500k_load_hmm_model --model ${HMM_MODEL} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_hmm_model.log +macs3 hmmratac -i $ATACSEQBAM -n hmmratac_yeast500k_load_hmm_model --model ${HMM_MODEL} --outdir ${OUTPUTDIR_PREFIX}_run_hmmratac &> ${OUTPUTDIR_PREFIX}_run_hmmratac/run_hmmratac_load_hmm_model.log echo "16. search for errors or warnings in log files" flag=0 diff --git a/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak b/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak new file mode 100644 index 00000000..642c94fd --- /dev/null +++ b/test/standard_results_hmmratac/hmmratac_yeast500k_bedpe_accessible_regions.gappedPeak @@ -0,0 +1,920 @@ +track name="peak" description="peak" type=gappedPeak nextItemButton=on +chrI 0 700 peak_1 0 . 20 560 0 1 540 20 0 0 0 +chrI 31650 34930 peak_2 0 . 32860 33100 0 1 240 1210 0 0 0 +chrI 45210 45620 peak_3 0 . 45290 45610 0 1 320 80 0 0 0 +chrI 60990 62170 peak_4 0 . 61100 61820 0 2 310,270 110,560 0 0 0 +chrI 62620 63520 peak_5 0 . 62630 62750 0 1 120 10 0 0 0 +chrI 67960 69560 peak_6 0 . 68170 69170 0 1 1000 210 0 0 0 +chrI 70980 71590 peak_7 0 . 71080 71500 0 1 420 100 0 0 0 +chrI 113440 113990 peak_8 0 . 113730 113870 0 1 140 290 0 0 0 +chrI 139050 139300 peak_9 0 . 139070 139250 0 1 180 20 0 0 0 +chrI 140490 142050 peak_10 0 . 141060 141970 0 2 210,430 570,1050 0 0 0 +chrI 165220 165900 peak_11 0 . 165400 165840 0 1 440 180 0 0 0 +chrI 166010 166460 peak_12 0 . 166190 166380 0 1 190 180 0 0 0 +chrI 180720 181650 peak_13 0 . 181040 181310 0 1 270 320 0 0 0 +chrI 182150 182740 peak_14 0 . 182430 182670 0 1 240 280 0 0 0 +chrI 189840 192080 peak_15 0 . 190020 191780 0 2 1610,110 180,1830 0 0 0 +chrI 210910 211290 peak_16 0 . 211070 211190 0 1 120 160 0 0 0 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324310 324440 0 1 130 50 0 0 0 +chrIX 325660 326210 peak_229 0 . 325740 325870 0 1 130 80 0 0 0 +chrIX 332620 333490 peak_230 0 . 332690 333480 0 1 790 70 0 0 0 +chrIX 381890 382830 peak_231 0 . 382190 382730 0 2 300,170 300,670 0 0 0 +chrIX 383760 384880 peak_232 0 . 384230 384530 0 1 300 470 0 0 0 +chrIX 386010 387070 peak_233 0 . 386550 386920 0 1 370 540 0 0 0 +chrIX 388430 388810 peak_234 0 . 388550 388690 0 1 140 120 0 0 0 +chrIX 439270 439870 peak_235 0 . 439290 439860 0 1 570 20 0 0 0 +chrM 6090 6240 peak_236 0 . 6100 6230 0 1 130 10 0 0 0 +chrM 14710 14870 peak_237 0 . 14720 14830 0 1 110 10 0 0 0 +chrM 16060 16270 peak_238 0 . 16090 16260 0 1 170 30 0 0 0 +chrM 17840 18450 peak_239 0 . 17860 18410 0 2 110,380 20,190 0 0 0 +chrM 18520 19010 peak_240 0 . 18590 19000 0 1 410 70 0 0 0 +chrM 19430 19560 peak_241 0 . 19440 19550 0 1 110 10 0 0 0 +chrM 20000 20380 peak_242 0 . 20010 20370 0 1 360 10 0 0 0 +chrM 21250 21410 peak_243 0 . 21280 21390 0 1 110 30 0 0 0 +chrM 21980 22260 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30 0 0 0 +chrM 79190 80040 peak_261 0 . 79200 80000 0 1 800 10 0 0 0 +chrV 6030 6400 peak_262 0 . 6210 6390 0 1 180 180 0 0 0 +chrV 41850 42710 peak_263 0 . 42060 42300 0 1 240 210 0 0 0 +chrV 53270 53800 peak_264 0 . 53350 53790 0 1 440 80 0 0 0 +chrV 68090 69760 peak_265 0 . 68970 69680 0 1 710 880 0 0 0 +chrV 69840 70300 peak_266 0 . 69980 70230 0 1 250 140 0 0 0 +chrV 84850 86210 peak_267 0 . 84930 85900 0 2 700,170 80,880 0 0 0 +chrV 86390 87790 peak_268 0 . 86400 86730 0 1 330 10 0 0 0 +chrV 106930 107110 peak_269 0 . 106960 107080 0 1 120 30 0 0 0 +chrV 117570 118240 peak_270 0 . 117690 118170 0 1 480 120 0 0 0 +chrV 123110 123990 peak_271 0 . 123160 123570 0 1 410 50 0 0 0 +chrV 140500 141490 peak_272 0 . 140520 141410 0 1 890 20 0 0 0 +chrV 174560 175020 peak_273 0 . 174710 174940 0 1 230 150 0 0 0 +chrV 191640 193720 peak_274 0 . 191880 192650 0 1 770 240 0 0 0 +chrV 212070 212360 peak_275 0 . 212110 212230 0 1 120 40 0 0 0 +chrV 221440 221840 peak_276 0 . 221620 221770 0 1 150 180 0 0 0 +chrV 221920 223150 peak_277 0 . 222250 222560 0 1 310 330 0 0 0 +chrV 225430 225720 peak_278 0 . 225440 225690 0 1 250 10 0 0 0 +chrV 241960 242700 peak_279 0 . 242160 242530 0 1 370 200 0 0 0 +chrV 268390 269130 peak_280 0 . 268420 268560 0 1 140 30 0 0 0 +chrV 270250 270600 peak_281 0 . 270290 270520 0 1 230 40 0 0 0 +chrV 284440 285090 peak_282 0 . 284510 285050 0 1 540 70 0 0 0 +chrV 303400 303850 peak_283 0 . 303630 303750 0 1 120 230 0 0 0 +chrV 305910 306270 peak_284 0 . 305940 306260 0 1 320 30 0 0 0 +chrV 311180 312200 peak_285 0 . 311510 312160 0 1 650 330 0 0 0 +chrV 334590 336790 peak_286 0 . 335420 335980 0 1 560 830 0 0 0 +chrV 342470 343100 peak_287 0 . 342480 342800 0 1 320 10 0 0 0 +chrV 349130 350180 peak_288 0 . 349450 349850 0 1 400 320 0 0 0 +chrV 354850 355330 peak_289 0 . 354860 355240 0 1 380 10 0 0 0 +chrV 362310 362630 peak_290 0 . 362380 362610 0 1 230 70 0 0 0 +chrV 386910 387460 peak_291 0 . 386920 387100 0 1 180 10 0 0 0 +chrV 395640 396680 peak_292 0 . 396320 396660 0 1 340 680 0 0 0 +chrV 399330 401380 peak_293 0 . 400760 401080 0 1 320 1430 0 0 0 +chrV 422470 423180 peak_294 0 . 422550 422800 0 1 250 80 0 0 0 +chrV 430700 431460 peak_295 0 . 431180 431450 0 1 270 480 0 0 0 +chrV 434410 434690 peak_296 0 . 434420 434680 0 1 260 10 0 0 0 +chrV 435500 435890 peak_297 0 . 435580 435790 0 1 210 80 0 0 0 +chrV 438780 439770 peak_298 0 . 439290 439400 0 1 110 510 0 0 0 +chrV 441600 442770 peak_299 0 . 441890 442530 0 1 640 290 0 0 0 +chrV 443130 443350 peak_300 0 . 443230 443340 0 1 110 100 0 0 0 +chrV 448420 449030 peak_301 0 . 448540 448990 0 1 450 120 0 0 0 +chrV 461150 462850 peak_302 0 . 461530 462460 0 2 690,130 380,1180 0 0 0 +chrV 473210 475830 peak_303 0 . 473500 475050 0 2 300,970 290,870 0 0 0 +chrV 490400 491430 peak_304 0 . 490950 491400 0 1 450 550 0 0 0 +chrV 492250 492490 peak_305 0 . 492280 492430 0 1 150 30 0 0 0 +chrV 497370 498140 peak_306 0 . 497740 497910 0 1 170 370 0 0 0 +chrV 544450 545350 peak_307 0 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190850 191670 peak_338 0 . 190920 191080 0 1 160 70 0 0 0 +chrVII 196680 197550 peak_339 0 . 197130 197440 0 1 310 450 0 0 0 +chrVII 205440 206030 peak_340 0 . 205530 205670 0 1 140 90 0 0 0 +chrVII 206400 206690 peak_341 0 . 206450 206650 0 1 200 50 0 0 0 +chrVII 254220 254970 peak_342 0 . 254340 254480 0 1 140 120 0 0 0 +chrVII 270220 272250 peak_343 0 . 270880 272230 0 1 1350 660 0 0 0 +chrVII 272440 273590 peak_344 0 . 272740 273180 0 1 440 300 0 0 0 +chrVII 276380 277580 peak_345 0 . 277130 277420 0 1 290 750 0 0 0 +chrVII 287040 287530 peak_346 0 . 287290 287520 0 1 230 250 0 0 0 +chrVII 305970 307060 peak_347 0 . 306350 306610 0 1 260 380 0 0 0 +chrVII 310610 310870 peak_348 0 . 310620 310840 0 1 220 10 0 0 0 +chrVII 319630 320080 peak_349 0 . 319700 319940 0 1 240 70 0 0 0 +chrVII 324630 325050 peak_350 0 . 324730 324880 0 1 150 100 0 0 0 +chrVII 371260 371640 peak_351 0 . 371290 371590 0 1 300 30 0 0 0 +chrVII 383400 385190 peak_352 0 . 384270 385040 0 1 770 870 0 0 0 +chrVII 401350 401700 peak_353 0 . 401430 401670 0 1 240 80 0 0 0 +chrVII 405320 406000 peak_354 0 . 405510 405650 0 1 140 190 0 0 0 +chrVII 412810 413590 peak_355 0 . 413250 413490 0 1 240 440 0 0 0 +chrVII 423010 423490 peak_356 0 . 423100 423320 0 1 220 90 0 0 0 +chrVII 428120 428400 peak_357 0 . 428140 428390 0 1 250 20 0 0 0 +chrVII 438320 438810 peak_358 0 . 438500 438770 0 1 270 180 0 0 0 +chrVII 483000 484200 peak_359 0 . 483280 483880 0 1 600 280 0 0 0 +chrVII 515780 516550 peak_360 0 . 516150 516520 0 1 370 370 0 0 0 +chrVII 527820 529080 peak_361 0 . 528350 528790 0 2 110,160 530,810 0 0 0 +chrVII 536030 536800 peak_362 0 . 536130 536480 0 1 350 100 0 0 0 +chrVII 546470 547870 peak_363 0 . 546960 547660 0 1 700 490 0 0 0 +chrVII 555440 555840 peak_364 0 . 555480 555670 0 1 190 40 0 0 0 +chrVII 559920 560470 peak_365 0 . 560140 560450 0 1 310 220 0 0 0 +chrVII 566770 567500 peak_366 0 . 567060 567310 0 1 250 290 0 0 0 +chrVII 576430 576910 peak_367 0 . 576620 576800 0 1 180 190 0 0 0 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40 0 0 0 +chrVII 883920 884410 peak_398 0 . 883970 884380 0 1 410 50 0 0 0 +chrVII 904940 906540 peak_399 0 . 905010 905810 0 1 800 70 0 0 0 +chrVII 914850 915600 peak_400 0 . 914970 915150 0 1 180 120 0 0 0 +chrVII 920120 920640 peak_401 0 . 920140 920540 0 1 400 20 0 0 0 +chrVII 974260 974770 peak_402 0 . 974530 974740 0 1 210 270 0 0 0 +chrVII 976750 977190 peak_403 0 . 976860 977090 0 1 230 110 0 0 0 +chrVII 986970 987820 peak_404 0 . 987010 987630 0 1 620 40 0 0 0 +chrVII 993790 994650 peak_405 0 . 993990 994280 0 1 290 200 0 0 0 +chrVII 999990 1002190 peak_406 0 . 1000020 1001420 0 2 740,460 30,970 0 0 0 +chrVII 1025950 1027480 peak_407 0 . 1026000 1027210 0 2 210,500 50,760 0 0 0 +chrVII 1050230 1050630 peak_408 0 . 1050320 1050540 0 1 220 90 0 0 0 +chrVII 1052220 1052480 peak_409 0 . 1052310 1052470 0 1 160 90 0 0 0 +chrVII 1083720 1084850 peak_410 0 . 1083730 1084670 0 1 940 10 0 0 0 +chrVII 1086710 1087830 peak_411 0 . 1087030 1087490 0 1 460 320 0 0 0 +chrVIII 4610 5410 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