diff --git a/.github/workflows/main.yml b/.github/workflows/main.yml
index 754054a812..8d15034063 100644
--- a/.github/workflows/main.yml
+++ b/.github/workflows/main.yml
@@ -259,6 +259,12 @@ jobs:
           pip install git+https://github.com/conda/conda-content-trust.git@main
 
           ./testserver.sh
+
+      - name: Shell init
+        shell: bash -l {0} -euo pipefail -x
+        run: |
+          mamba init
+
       - name: Cleanup
         shell: bash
         if: always()
diff --git a/mamba/mamba/mamba_shell_init.py b/mamba/mamba/mamba_shell_init.py
index 965ce69c4d..03f22e94bf 100644
--- a/mamba/mamba/mamba_shell_init.py
+++ b/mamba/mamba/mamba_shell_init.py
@@ -84,7 +84,8 @@ def shell_init(args):
         for_user = args.user
         if not (args.install and args.user and args.system):
             for_user = True
-        if args.no_user:
+        # no_user removed in Conda 23.1.0, see https://github.com/mamba-org/mamba/issues/2248
+        if hasattr(args, "no_user") and args.no_user:
             for_user = False
 
         anaconda_prompt = on_win and args.anaconda_prompt