diff --git a/pyproject.toml b/pyproject.toml
index 50e46261b..e67a22f59 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -93,7 +93,7 @@ tests = [
     "pytest-mock==3.15.1",
     "pytest-split==0.10.0",
     "pytest-xdist==3.8.0",
-    "pytest==8.4.2",
+    "pytest==9.0.1",
 ]
 strict-openff = [
     "mdanalysis==2.9.0",