diff --git a/changelog.d/10002.bugfix b/changelog.d/10002.bugfix
new file mode 100644
index 000000000000..1fabdad22eee
--- /dev/null
+++ b/changelog.d/10002.bugfix
@@ -0,0 +1 @@
+Fix a validation bug introduced in v1.34.0 in the ordering of spaces in the space summary API.
diff --git a/synapse/handlers/space_summary.py b/synapse/handlers/space_summary.py
index 953356f34d90..eb80a5ad67db 100644
--- a/synapse/handlers/space_summary.py
+++ b/synapse/handlers/space_summary.py
@@ -471,8 +471,8 @@ def _is_suggested_child_event(edge_event: EventBase) -> bool:
     return False
 
 
-# Order may only contain characters in the range of \x20 (space) to \x7F (~).
-_INVALID_ORDER_CHARS_RE = re.compile(r"[^\x20-\x7F]")
+# Order may only contain characters in the range of \x20 (space) to \x7E (~) inclusive.
+_INVALID_ORDER_CHARS_RE = re.compile(r"[^\x20-\x7E]")
 
 
 def _child_events_comparison_key(child: EventBase) -> Tuple[bool, Optional[str], str]: