diff --git a/.github/workflows/download-arm64-libs.py b/.github/workflows/download-arm64-libs.py
index 63e48971a..21b23cc79 100644
--- a/.github/workflows/download-arm64-libs.py
+++ b/.github/workflows/download-arm64-libs.py
@@ -21,11 +21,11 @@
 
 VERSION = "{}.{}.{}".format(*sys.version_info[:3])
 if sys.version_info.releaselevel == "alpha":
-    VERSION += "-a{}".format(sys.version_info.serial)
+    VERSION += f"-a{sys.version_info.serial}"
 if sys.version_info.releaselevel == "beta":
-    VERSION += "-b{}".format(sys.version_info.serial)
+    VERSION += f"-b{sys.version_info.serial}"
 if sys.version_info.releaselevel == "candidate":
-    VERSION += "-rc{}".format(sys.version_info.serial)
+    VERSION += f"-rc{sys.version_info.serial}"
 
 URL = f"https://www.nuget.org/api/v2/package/pythonarm64/{VERSION}"
 PATH = dest / f"pythonarm64.{VERSION}.zip"
diff --git a/.github/workflows/main.yml b/.github/workflows/main.yml
index 260d16047..fe4a75f6a 100644
--- a/.github/workflows/main.yml
+++ b/.github/workflows/main.yml
@@ -99,7 +99,11 @@ jobs:
     runs-on: ubuntu-latest
     steps:
       - uses: actions/checkout@v3
-      - run: pip install isort pycln
+      - run: pip install pyupgrade pycln isort
+      # Ignore adodbapi until this has been manually ran and merged in adodbapi code
+      # _dbgscript.py is non-UTF8 on purpose, which is not supported
+      # TODO: Remove the --keep-percent-format flag and fix all printf-style string formatting
+      - run: pyupgrade --keep-percent-format --py37-plus $(git ls-files '**.py*' ':!:adodbapi/*' ':!:Pythonwin/pywin/test/_dbgscript.py')
       - run: pycln . --config=pycln.toml --check
       - run: isort . --diff --check-only
       - uses: psf/black@stable
diff --git a/AutoDuck/Dump2HHC.py b/AutoDuck/Dump2HHC.py
index d6071d31b..30c49fbd7 100644
--- a/AutoDuck/Dump2HHC.py
+++ b/AutoDuck/Dump2HHC.py
@@ -465,7 +465,7 @@ def main():
     cats = parseCategories()
     for cat in cats:
         file = os.path.join(gen_dir, cat.dump_file)
-        input = open(file, "r")
+        input = open(file)
         parseTopics(cat, input)
         input.close()
 
diff --git a/AutoDuck/InsertExternalOverviews.py b/AutoDuck/InsertExternalOverviews.py
index c94ddef44..22d228310 100644
--- a/AutoDuck/InsertExternalOverviews.py
+++ b/AutoDuck/InsertExternalOverviews.py
@@ -51,7 +51,7 @@ def main():
         print("Invalid args")
         sys.exit(1)
     file = sys.argv[1]
-    input = open(file, "r")
+    input = open(file)
     out = open(file + ".2", "w")
     doc = document_object.GetDocument()
     linksHTML = genLinksHTML(doc.links)
diff --git a/AutoDuck/TOCToHHK.py b/AutoDuck/TOCToHHK.py
index 235b78677..a91ec7547 100644
--- a/AutoDuck/TOCToHHK.py
+++ b/AutoDuck/TOCToHHK.py
@@ -11,7 +11,7 @@
 def main():
     file = sys.argv[1]
     output = sys.argv[2]
-    input = open(file, "r")
+    input = open(file)
     out = open(output, "w")
     line = input.readline()
     out.write(
diff --git a/Pythonwin/Scintilla/src/LexGen.py b/Pythonwin/Scintilla/src/LexGen.py
index 7c1d9eb0b..08a0e7854 100644
--- a/Pythonwin/Scintilla/src/LexGen.py
+++ b/Pythonwin/Scintilla/src/LexGen.py
@@ -135,7 +135,7 @@ def Generate(inpath, outpath, commentPrefix, eolType, *lists):
     # print "generate '%s' -> '%s' (comment prefix: %r, eols: %r)"\
     #      % (inpath, outpath, commentPrefix, eolType)
     try:
-        infile = open(inpath, "r")
+        infile = open(inpath)
     except OSError:
         print("Can not open", inpath)
         return
diff --git a/Pythonwin/pywin/framework/mdi_pychecker.py b/Pythonwin/pywin/framework/mdi_pychecker.py
index 06fbcc82b..2b841befc 100644
--- a/Pythonwin/pywin/framework/mdi_pychecker.py
+++ b/Pythonwin/pywin/framework/mdi_pychecker.py
@@ -210,7 +210,7 @@ def OnOpenDocument(self, fnm):
         # and starting a new grep can communicate the default parameters to the
         # new grep.
         try:
-            params = open(fnm, "r").read()
+            params = open(fnm).read()
         except:
             params = None
         self.setInitParams(params)
@@ -386,7 +386,7 @@ def _inactive_idleHandler(self, handler, count):
             if self.verbose:
                 self.GetFirstView().Append("# .." + f + "\n")
             win32ui.SetStatusText("Searching " + f, 0)
-            lines = open(f, "r").readlines()
+            lines = open(f).readlines()
             for i in range(len(lines)):
                 line = lines[i]
                 if self.pat.search(line) is not None:
diff --git a/Pythonwin/pywin/framework/scriptutils.py b/Pythonwin/pywin/framework/scriptutils.py
index c6e8e3244..97b784a56 100644
--- a/Pythonwin/pywin/framework/scriptutils.py
+++ b/Pythonwin/pywin/framework/scriptutils.py
@@ -101,8 +101,8 @@ def IsOnPythonPath(path):
 
 def GetPackageModuleName(fileName):
     """Given a filename, return (module name, new path).
-    eg - given "c:\a\b\c\my.py", return ("b.c.my",None) if "c:\a" is on sys.path.
-    If no package found, will return ("my", "c:\a\b\c")
+    eg - given "c:\a\b\\c\\my.py", return ("b.c.my",None) if "c:\a" is on sys.path.
+    If no package found, will return ("my", "c:\a\b\\c")
     """
     path, fname = os.path.split(fileName)
     path = origPath = win32ui.FullPath(path)
diff --git a/Pythonwin/pywin/framework/sgrepmdi.py b/Pythonwin/pywin/framework/sgrepmdi.py
index 16bff7fa8..5a9260145 100644
--- a/Pythonwin/pywin/framework/sgrepmdi.py
+++ b/Pythonwin/pywin/framework/sgrepmdi.py
@@ -191,7 +191,7 @@ def OnOpenDocument(self, fnm):
         # and starting a new grep can communicate the default parameters to the
         # new grep.
         try:
-            params = open(fnm, "r").read()
+            params = open(fnm).read()
         except:
             params = None
         self.setInitParams(params)
@@ -299,7 +299,7 @@ def SearchFile(self, handler, count):
             #  while grep is running
             if os.path.isfile(f):
                 win32ui.SetStatusText("Searching " + f, 0)
-                lines = open(f, "r").readlines()
+                lines = open(f).readlines()
                 for i in range(len(lines)):
                     line = lines[i]
                     if self.pat.search(line) is not None:
diff --git a/Pythonwin/pywin/tools/__init__.py b/Pythonwin/pywin/tools/__init__.py
index 8b1378917..e69de29bb 100644
--- a/Pythonwin/pywin/tools/__init__.py
+++ b/Pythonwin/pywin/tools/__init__.py
@@ -1 +0,0 @@
-
diff --git a/com/win32com/client/genpy.py b/com/win32com/client/genpy.py
index 96997d3a2..f1aef3575 100644
--- a/com/win32com/client/genpy.py
+++ b/com/win32com/client/genpy.py
@@ -1036,7 +1036,7 @@ def open_writer(self, filename, encoding="mbcs"):
         # don't step on each others' toes.
         # Could be a classmethod one day...
         temp_filename = self.get_temp_filename(filename)
-        return open(temp_filename, "wt", encoding=encoding)
+        return open(temp_filename, "w", encoding=encoding)
 
     def finish_writer(self, filename, f, worked):
         f.close()
diff --git a/com/win32com/client/makepy.py b/com/win32com/client/makepy.py
index bb1bf4e07..f041062eb 100644
--- a/com/win32com/client/makepy.py
+++ b/com/win32com/client/makepy.py
@@ -425,7 +425,7 @@ def main():
         path = os.path.dirname(outputName)
         if path != "" and not os.path.exists(path):
             os.makedirs(path)
-        f = open(outputName, "wt", encoding="mbcs")
+        f = open(outputName, "w", encoding="mbcs")
 
     else:
         f = None
diff --git a/com/win32com/demos/iebutton.py b/com/win32com/demos/iebutton.py
index 6ded24c9f..d870c21cd 100644
--- a/com/win32com/demos/iebutton.py
+++ b/com/win32com/demos/iebutton.py
@@ -1,5 +1,3 @@
-# -*- coding: latin-1 -*-
-
 # PyWin32 Internet Explorer Button
 #
 # written by Leonard Ritter (paniq@gmx.net)
diff --git a/com/win32com/demos/ietoolbar.py b/com/win32com/demos/ietoolbar.py
index b520dcdad..22b69011b 100644
--- a/com/win32com/demos/ietoolbar.py
+++ b/com/win32com/demos/ietoolbar.py
@@ -1,5 +1,3 @@
-# -*- coding: latin-1 -*-
-
 # PyWin32 Internet Explorer Toolbar
 #
 # written by Leonard Ritter (paniq@gmx.net)
diff --git a/com/win32com/test/testIterators.py b/com/win32com/test/testIterators.py
index 8eba364a7..bc310ce83 100644
--- a/com/win32com/test/testIterators.py
+++ b/com/win32com/test/testIterators.py
@@ -132,7 +132,7 @@ def suite():
             and issubclass(item, unittest.TestCase)
             and item != _BaseTestCase
         ):
-            suite.addTest(unittest.makeSuite(item))
+            suite.addTest(unittest.defaultTestLoader.loadTestsFromTestCase(item))
     return suite
 
 
diff --git a/com/win32com/test/testall.py b/com/win32com/test/testall.py
index 6ca312e34..abebaecea 100644
--- a/com/win32com/test/testall.py
+++ b/com/win32com/test/testall.py
@@ -218,7 +218,7 @@ def make_test_suite(test_level=1):
         for mod_name in unittest_modules[i]:
             mod, func = get_test_mod_and_func(mod_name, import_failures)
             if mod is None:
-                raise Exception("no such module '{}'".format(mod_name))
+                raise Exception(f"no such module '{mod_name}'")
             if func is not None:
                 test = CapturingFunctionTestCase(func, description=mod_name)
             else:
diff --git a/com/win32com/test/util.py b/com/win32com/test/util.py
index 1704f6326..3a7cdd706 100644
--- a/com/win32com/test/util.py
+++ b/com/win32com/test/util.py
@@ -104,7 +104,7 @@ def ExecuteShellCommand(
     output_name = tempfile.mktemp("win32com_test")
     cmd = cmd + ' > "%s" 2>&1' % output_name
     rc = os.system(cmd)
-    output = open(output_name, "r").read().strip()
+    output = open(output_name).read().strip()
     os.remove(output_name)
 
     class Failed(Exception):
diff --git a/com/win32comext/authorization/demos/EditServiceSecurity.py b/com/win32comext/authorization/demos/EditServiceSecurity.py
index 009f4245c..7f6233e31 100644
--- a/com/win32comext/authorization/demos/EditServiceSecurity.py
+++ b/com/win32comext/authorization/demos/EditServiceSecurity.py
@@ -1,7 +1,7 @@
 """
 Implements a permissions editor for services.
 Service can be specified as plain name for local machine,
-or as a remote service of the form \\machinename\service
+or as a remote service of the form \\machinename\\service
 """
 
 import os
diff --git a/com/win32comext/axdebug/Test/host.py b/com/win32comext/axdebug/Test/host.py
index da99b1b2f..73ed6f31c 100644
--- a/com/win32comext/axdebug/Test/host.py
+++ b/com/win32comext/axdebug/Test/host.py
@@ -57,7 +57,7 @@ def _query_interface_(self, iid):
     def _GetCodeContainer(self):
         if self.codeContainer is None:
             try:
-                codeText = open(self.module.__file__, "rt").read()
+                codeText = open(self.module.__file__).read()
             except OSError as details:
                 codeText = "# Exception opening file\n# %s" % (details)
 
diff --git a/com/win32comext/axdebug/codecontainer.py b/com/win32comext/axdebug/codecontainer.py
index be20a2d39..ac99fe9e4 100644
--- a/com/win32comext/axdebug/codecontainer.py
+++ b/com/win32comext/axdebug/codecontainer.py
@@ -224,7 +224,7 @@ def GetText(self):
             fname = self.GetFileName()
             if fname:
                 try:
-                    self.text = open(fname, "r").read()
+                    self.text = open(fname).read()
                 except OSError as details:
                     self.text = "# Exception opening file\n# %s" % (repr(details))
             else:
diff --git a/format_all.bat b/format_all.bat
index 10b8aeddb..e8b440d06 100644
--- a/format_all.bat
+++ b/format_all.bat
@@ -1,3 +1,11 @@
+@echo off
+@REM Ignore adodbapi until this has been manually ran and merged in adodbapi code
+@REM _dbgscript.py is non-UTF8 on purpose, which is not supported
+for /f "delims=" %%F in ('git ls-files **.py* :!:adodbapi/* :!:Pythonwin/pywin/test/_dbgscript.py') do (
+  @REM TODO: Remove the --keep-percent-format flag and fix all printf-style string formatting
+  pyupgrade --keep-percent-format --py37-plus %%F
+)
+@echo on
 pycln . --config=pycln.toml
 isort .
 black .
diff --git a/pywin32_postinstall.py b/pywin32_postinstall.py
index 1c0b396ad..101c99f43 100644
--- a/pywin32_postinstall.py
+++ b/pywin32_postinstall.py
@@ -692,7 +692,7 @@ def uninstall(lib_dir):
 
 def verify_destination(location):
     if not os.path.isdir(location):
-        raise argparse.ArgumentTypeError('Path "{}" does not exist!'.format(location))
+        raise argparse.ArgumentTypeError(f'Path "{location}" does not exist!')
     return location
 
 
@@ -756,7 +756,7 @@ def main():
     args = parser.parse_args()
 
     if not args.quiet:
-        print("Parsed arguments are: {}".format(args))
+        print(f"Parsed arguments are: {args}")
 
     if not args.install ^ args.remove:
         parser.error("You need to either choose to -install or -remove!")
diff --git a/setup.py b/setup.py
index 7309fc119..9e6bb37da 100644
--- a/setup.py
+++ b/setup.py
@@ -543,7 +543,7 @@ def _check_vc(self):
         # so find and add them
         if vcbase and not atlmfc_found:
             atls_lib = glob.glob(
-                vcbase + r"ATLMFC\lib\{}\atls.lib".format(self.plat_dir)
+                vcbase + fr"ATLMFC\lib\{self.plat_dir}\atls.lib"
             )
             if atls_lib:
                 self.library_dirs.append(os.path.dirname(atls_lib[0]))
diff --git a/win32/Demos/EvtFormatMessage.py b/win32/Demos/EvtFormatMessage.py
index 125438692..ecd5075a7 100644
--- a/win32/Demos/EvtFormatMessage.py
+++ b/win32/Demos/EvtFormatMessage.py
@@ -21,7 +21,7 @@ def main():
             event, win32evtlog.EvtRenderEventValues, Context=context
         )
 
-        print("Event {}".format(i))
+        print(f"Event {i}")
 
         level_value, level_variant = result[win32evtlog.EvtSystemLevel]
         if level_variant != win32evtlog.EvtVarTypeNull:
@@ -42,17 +42,17 @@ def main():
             win32evtlog.EvtSystemTimeCreated
         ]
         if time_created_variant != win32evtlog.EvtVarTypeNull:
-            print("    Timestamp: {}".format(time_created_value.isoformat()))
+            print(f"    Timestamp: {time_created_value.isoformat()}")
 
         computer_value, computer_variant = result[win32evtlog.EvtSystemComputer]
         if computer_variant != win32evtlog.EvtVarTypeNull:
-            print("    FQDN: {}".format(computer_value))
+            print(f"    FQDN: {computer_value}")
 
         provider_name_value, provider_name_variant = result[
             win32evtlog.EvtSystemProviderName
         ]
         if provider_name_variant != win32evtlog.EvtVarTypeNull:
-            print("    Provider: {}".format(provider_name_value))
+            print(f"    Provider: {provider_name_value}")
 
             try:
                 metadata = win32evtlog.EvtOpenPublisherMetadata(provider_name_value)
@@ -69,7 +69,7 @@ def main():
                     pass
                 else:
                     try:
-                        print("    Message: {}".format(message))
+                        print(f"    Message: {message}")
                     except UnicodeEncodeError:
                         # Obscure error when run under subprocess.Popen(), presumably due to
                         # not knowing the correct encoding for the console.
diff --git a/win32/Lib/win32rcparser.py b/win32/Lib/win32rcparser.py
index 627cea1ba..92a19e710 100644
--- a/win32/Lib/win32rcparser.py
+++ b/win32/Lib/win32rcparser.py
@@ -584,21 +584,21 @@ def ParseStreams(rc_file, h_file):
 
 def Parse(rc_name, h_name=None):
     if h_name:
-        h_file = open(h_name, "r")
+        h_file = open(h_name)
     else:
         # See if same basename as the .rc
         h_name = rc_name[:-2] + "h"
         try:
-            h_file = open(h_name, "r")
+            h_file = open(h_name)
         except OSError:
             # See if MSVC default of 'resource.h' in the same dir.
             h_name = os.path.join(os.path.dirname(rc_name), "resource.h")
             try:
-                h_file = open(h_name, "r")
+                h_file = open(h_name)
             except OSError:
                 # .h files are optional anyway
                 h_file = None
-    rc_file = open(rc_name, "r")
+    rc_file = open(rc_name)
     try:
         return ParseStreams(rc_file, h_file)
     finally:
@@ -617,7 +617,7 @@ def GenerateFrozenResource(rc_name, output_name, h_name=None):
     rcp = Parse(rc_name, h_name)
     in_stat = os.stat(rc_name)
 
-    out = open(output_name, "wt")
+    out = open(output_name, "w")
     out.write("#%s\n" % output_name)
     out.write("#This is a generated file. Please edit %s instead.\n" % rc_name)
     out.write("__version__=%r\n" % __version__)
diff --git a/win32/Lib/win32timezone.py b/win32/Lib/win32timezone.py
index 442cb1f6d..ee9cfecca 100644
--- a/win32/Lib/win32timezone.py
+++ b/win32/Lib/win32timezone.py
@@ -1,5 +1,3 @@
-# -*- coding: UTF-8 -*-
-
 """
 win32timezone:
     Module for handling datetime.tzinfo time zones using the windows
diff --git a/win32/scripts/VersionStamp/bulkstamp.py b/win32/scripts/VersionStamp/bulkstamp.py
index 44f2a63e0..297f4adca 100644
--- a/win32/scripts/VersionStamp/bulkstamp.py
+++ b/win32/scripts/VersionStamp/bulkstamp.py
@@ -85,7 +85,7 @@ def load_descriptions(fname, vars):
     retvars = {}
     descriptions = {}
 
-    lines = open(fname, "r").readlines()
+    lines = open(fname).readlines()
 
     for i in range(len(lines)):
         line = lines[i].strip()
diff --git a/win32/scripts/VersionStamp/vssutil.py b/win32/scripts/VersionStamp/vssutil.py
index 6679ebc93..1e970d817 100644
--- a/win32/scripts/VersionStamp/vssutil.py
+++ b/win32/scripts/VersionStamp/vssutil.py
@@ -56,7 +56,7 @@ def SubstituteInString(inString, evalEnv):
 
 
 def SubstituteInFile(inName, outName, evalEnv):
-    inFile = open(inName, "r")
+    inFile = open(inName)
     try:
         outFile = open(outName, "w")
         try:
diff --git a/win32/scripts/regsetup.py b/win32/scripts/regsetup.py
index 20a4f8471..1c17736f2 100644
--- a/win32/scripts/regsetup.py
+++ b/win32/scripts/regsetup.py
@@ -506,9 +506,9 @@ def RegisterShellInfo(searchPaths):
 as well as the 2 wierd spots to locate the core Python files (eg, Python.exe,
 pythonXX.dll, the standard library and Win32 Extensions.
 
-"regsetup -a myappname . .\subdir"
+"regsetup -a myappname . .\\subdir"
 Registers a new Pythonpath entry named myappname, with "C:\\I\\AM\\HERE" and
-"C:\\I\\AM\\HERE\subdir" added to the path (ie, all args are converted to
+"C:\\I\\AM\\HERE\\subdir" added to the path (ie, all args are converted to
 absolute paths)
 
 "regsetup -c c:\\my\\python\\files"
diff --git a/win32/test/test_odbc.py b/win32/test/test_odbc.py
index 8cba6310f..1d2a9f1b2 100644
--- a/win32/test/test_odbc.py
+++ b/win32/test/test_odbc.py
@@ -206,7 +206,7 @@ def testLongBinary(self):
 
     def testRaw(self):
         ## Test binary data
-        self._test_val("rawfield", memoryview(b"\1\2\3\4\0\5\6\7\8"))
+        self._test_val("rawfield", memoryview(b"\1\2\3\4\0\5\6\7\\8"))
 
     def test_widechar(self):
         """Test a unicode character that would be mangled if bound as plain character.
diff --git a/win32/test/testall.py b/win32/test/testall.py
index 85adf736e..373dd212b 100644
--- a/win32/test/testall.py
+++ b/win32/test/testall.py
@@ -85,7 +85,7 @@ def find_exception_in_output(data):
 class TestRunner:
     def __init__(self, argv):
         self.argv = argv
-        self.__name__ = "Test Runner for cmdline {}".format(argv)
+        self.__name__ = f"Test Runner for cmdline {argv}"
 
     def __call__(self):
         import subprocess