diff --git a/change/@fluentui-react-internal-2020-10-23-11-25-33-fix-ref-conformance-react-internal-separator.json b/change/@fluentui-react-internal-2020-10-23-11-25-33-fix-ref-conformance-react-internal-separator.json
new file mode 100644
index 0000000000000..f3ae705db26eb
--- /dev/null
+++ b/change/@fluentui-react-internal-2020-10-23-11-25-33-fix-ref-conformance-react-internal-separator.json
@@ -0,0 +1,8 @@
+{
+  "type": "prerelease",
+  "comment": "Adding a forwardRef to Separator within react-internal.",
+  "packageName": "@fluentui/react-internal",
+  "email": "czearing@outlook.com",
+  "dependentChangeType": "patch",
+  "date": "2020-10-23T18:25:33.495Z"
+}
diff --git a/packages/react-internal/etc/react-internal.api.md b/packages/react-internal/etc/react-internal.api.md
index d9e0e047cea5e..3e3bf35311d77 100644
--- a/packages/react-internal/etc/react-internal.api.md
+++ b/packages/react-internal/etc/react-internal.api.md
@@ -4386,7 +4386,7 @@ export interface ISeparator {
 }
 
 // @public (undocumented)
-export interface ISeparatorProps extends React.HTMLAttributes<HTMLElement> {
+export interface ISeparatorProps extends React.HTMLAttributes<HTMLDivElement>, React.RefAttributes<HTMLDivElement> {
     alignContent?: 'start' | 'center' | 'end';
     styles?: IStyleFunctionOrObject<ISeparatorStyleProps, ISeparatorStyles>;
     theme?: ITheme;
diff --git a/packages/react-internal/src/components/Separator/Separator.base.tsx b/packages/react-internal/src/components/Separator/Separator.base.tsx
index dbf49552f2e62..b89502d756d17 100644
--- a/packages/react-internal/src/components/Separator/Separator.base.tsx
+++ b/packages/react-internal/src/components/Separator/Separator.base.tsx
@@ -4,10 +4,13 @@ import { ISeparatorProps, ISeparatorStyles, ISeparatorStyleProps } from './Separ
 
 const getClassNames = classNamesFunction<ISeparatorStyleProps, ISeparatorStyles>();
 
-export const SeparatorBase: React.FunctionComponent<ISeparatorProps> = (props: ISeparatorProps): JSX.Element => {
-  const { styles, theme, className, vertical, alignContent } = props;
+export const SeparatorBase: React.FunctionComponent<ISeparatorProps> = React.forwardRef<
+  HTMLDivElement,
+  ISeparatorProps
+>((props, ref) => {
+  const { styles, theme, className, vertical, alignContent, children } = props;
 
-  const _classNames = getClassNames(styles!, {
+  const classNames = getClassNames(styles!, {
     theme: theme!,
     className,
     alignContent: alignContent,
@@ -15,10 +18,10 @@ export const SeparatorBase: React.FunctionComponent<ISeparatorProps> = (props: I
   });
 
   return (
-    <div className={_classNames.root}>
-      <div className={_classNames.content} role="separator" aria-orientation={vertical ? 'vertical' : 'horizontal'}>
-        {props.children}
+    <div className={classNames.root} ref={ref}>
+      <div className={classNames.content} role="separator" aria-orientation={vertical ? 'vertical' : 'horizontal'}>
+        {children}
       </div>
     </div>
   );
-};
+});
diff --git a/packages/react-internal/src/components/Separator/Separator.test.tsx b/packages/react-internal/src/components/Separator/Separator.test.tsx
index 105ea6857322b..a544e79d19133 100644
--- a/packages/react-internal/src/components/Separator/Separator.test.tsx
+++ b/packages/react-internal/src/components/Separator/Separator.test.tsx
@@ -5,8 +5,5 @@ describe('Separator', () => {
   isConformant({
     Component: Separator,
     displayName: 'Separator',
-    // Problem: Doesn’t pass ref to the root element.
-    // Solution: Add a ref to the root element.
-    disabledTests: ['component-has-root-ref', 'component-handles-ref'],
   });
 });
diff --git a/packages/react-internal/src/components/Separator/Separator.types.ts b/packages/react-internal/src/components/Separator/Separator.types.ts
index 9da58d42b5b23..bf6637560f9da 100644
--- a/packages/react-internal/src/components/Separator/Separator.types.ts
+++ b/packages/react-internal/src/components/Separator/Separator.types.ts
@@ -10,7 +10,7 @@ export interface ISeparator {}
 /**
  * {@docCategory Separator}
  */
-export interface ISeparatorProps extends React.HTMLAttributes<HTMLElement> {
+export interface ISeparatorProps extends React.HTMLAttributes<HTMLDivElement>, React.RefAttributes<HTMLDivElement> {
   /**
    * Theme (provided through customization.)
    */