diff --git a/.gitignore b/.gitignore
index c8db9e06..7a793131 100644
--- a/.gitignore
+++ b/.gitignore
@@ -18,4 +18,7 @@ tmp/
 temp/
 docs/node_modules/
 docs/package.json
-docs/package-lock.json
\ No newline at end of file
+docs/package-lock.json
+# Test-generated conda environments
+crates/pet-conda/tests/unix/env_with_python3/
+crates/pet-conda/tests/unix/env_without_python3/
diff --git a/crates/pet-conda/src/environment_locations.rs b/crates/pet-conda/src/environment_locations.rs
index 744da92c..a1ca53f5 100644
--- a/crates/pet-conda/src/environment_locations.rs
+++ b/crates/pet-conda/src/environment_locations.rs
@@ -412,6 +412,13 @@ pub fn get_known_conda_install_locations(
             known_paths.push(prefix.clone().join("miniconda3"));
             known_paths.push(prefix.clone().join("miniforge3"));
             known_paths.push(prefix.clone().join("micromamba"));
+            
+            // Homebrew cask installations are in the Caskroom subdirectory
+            if prefix == PathBuf::from("/opt/homebrew") {
+                known_paths.push(prefix.clone().join("Caskroom").join("miniforge").join("base"));
+                known_paths.push(prefix.clone().join("Caskroom").join("miniconda").join("base"));
+                known_paths.push(prefix.clone().join("Caskroom").join("anaconda").join("base"));
+            }
         }
 
         known_paths.push(PathBuf::from("/opt").join("conda"));
diff --git a/crates/pet-conda/tests/homebrew_base_test.rs b/crates/pet-conda/tests/homebrew_base_test.rs
new file mode 100644
index 00000000..7354c240
--- /dev/null
+++ b/crates/pet-conda/tests/homebrew_base_test.rs
@@ -0,0 +1,86 @@
+// Copyright (c) Microsoft Corporation. All rights reserved.
+// Licensed under the MIT License.
+
+//! Test to verify homebrew conda base environment discovery
+
+use pet_conda::environment_locations::get_known_conda_install_locations;
+use pet_core::os_environment::Environment;
+use std::{
+    collections::HashMap,
+    path::PathBuf,
+};
+
+/// Mock environment that simulates a macOS environment for testing
+struct MacOSMockEnvironment {
+    vars: HashMap<String, String>,
+}
+
+impl Environment for MacOSMockEnvironment {
+    fn get_user_home(&self) -> Option<PathBuf> {
+        Some(PathBuf::from("/Users/username"))
+    }
+
+    fn get_env_var(&self, key: String) -> Option<String> {
+        self.vars.get(&key).cloned()
+    }
+
+    fn get_know_global_search_locations(&self) -> Vec<PathBuf> {
+        vec![]
+    }
+
+    fn get_root(&self) -> Option<PathBuf> {
+        Some(PathBuf::from("/"))
+    }
+}
+
+#[test]
+fn test_homebrew_caskroom_paths_added_to_known_locations() {
+    // Test that homebrew caskroom paths are added to known conda install locations
+    // Note: This test verifies the logic without relying on actual OS detection
+    
+    let env = MacOSMockEnvironment {
+        vars: HashMap::new(),
+    };
+    
+    let env_vars = pet_conda::env_variables::EnvVariables::from(&env);
+    let conda_executable = None; // Test without executable to focus on known path discovery
+    
+    // Get known conda install locations
+    let known_locations = get_known_conda_install_locations(&env_vars, &conda_executable);
+    println!("Known conda install locations: {:?}", known_locations);
+    
+    // Check for common homebrew paths that should be included
+    let expected_homebrew_paths = if std::env::consts::OS == "macos" {
+        vec![
+            PathBuf::from("/opt/homebrew/Caskroom/miniforge/base"),
+            PathBuf::from("/opt/homebrew/Caskroom/miniconda/base"),
+            PathBuf::from("/opt/homebrew/Caskroom/anaconda/base"),
+        ]
+    } else {
+        // In Linux test environment, check that logic would work
+        // The actual paths won't be included since std::env::consts::OS != "macos"
+        vec![]
+    };
+    
+    for expected_path in expected_homebrew_paths {
+        assert!(
+            known_locations.contains(&expected_path),
+            "Homebrew caskroom path should be found in known conda install locations: {:?}. Known locations: {:?}",
+            expected_path,
+            known_locations
+        );
+    }
+    
+    // This test documents the expected behavior
+    println!("Homebrew caskroom path discovery test completed");
+    
+    // Even in Linux environment, we can document that the fix is in place
+    // by checking the code path would be triggered on macOS
+    if std::env::consts::OS == "macos" {
+        // On macOS, the homebrew paths should be included
+        assert!(!known_locations.is_empty(), "Known locations should not be empty on macOS");
+    } else {
+        // On Linux, we just document that this test ran
+        println!("Test ran on Linux - homebrew paths would be added on macOS");
+    }
+}
\ No newline at end of file