diff --git a/CHANGELOG.md b/CHANGELOG.md
index a2918e702c..daada653d5 100644
--- a/CHANGELOG.md
+++ b/CHANGELOG.md
@@ -14,6 +14,8 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0
 
 ### Changed
 
+- [#1339](https://github.com/nf-core/sarek/pull/1339) - Update sentieon-modules
+
 ### Fixed
 
 - [#1334](https://github.com/nf-core/sarek/pull/1334) - Remove extra v, when reporting tower runs on slack
diff --git a/modules.json b/modules.json
index dd5387fbb8..93e8ba3812 100644
--- a/modules.json
+++ b/modules.json
@@ -391,42 +391,42 @@
                     },
                     "sentieon/applyvarcal": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff",
                         "installed_by": ["modules"]
                     },
                     "sentieon/bwamem": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff",
                         "installed_by": ["modules"]
                     },
                     "sentieon/dedup": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff",
                         "installed_by": ["modules"]
                     },
                     "sentieon/dnamodelapply": {
                         "branch": "master",
-                        "git_sha": "214d575774c172062924ad3564b4f66655600730",
+                        "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff",
                         "installed_by": ["modules"]
                     },
                     "sentieon/dnascope": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff",
                         "installed_by": ["modules"]
                     },
                     "sentieon/gvcftyper": {
                         "branch": "master",
-                        "git_sha": "214d575774c172062924ad3564b4f66655600730",
+                        "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff",
                         "installed_by": ["modules"]
                     },
                     "sentieon/haplotyper": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff",
                         "installed_by": ["modules"]
                     },
                     "sentieon/varcal": {
                         "branch": "master",
-                        "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5",
+                        "git_sha": "89b6873f15dd31ed17f4d10ede2fa623e2a128ff",
                         "installed_by": ["modules"]
                     },
                     "snpeff/download": {
diff --git a/modules/nf-core/sentieon/applyvarcal/environment.yml b/modules/nf-core/sentieon/applyvarcal/environment.yml
index b7b5169a89..c4c11b1f85 100644
--- a/modules/nf-core/sentieon/applyvarcal/environment.yml
+++ b/modules/nf-core/sentieon/applyvarcal/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::sentieon=202308.01
diff --git a/modules/nf-core/sentieon/applyvarcal/main.nf b/modules/nf-core/sentieon/applyvarcal/main.nf
index 4e63b94ce4..304d0a0431 100644
--- a/modules/nf-core/sentieon/applyvarcal/main.nf
+++ b/modules/nf-core/sentieon/applyvarcal/main.nf
@@ -5,7 +5,10 @@ process SENTIEON_APPLYVARCAL {
 
     secret 'SENTIEON_LICENSE_BASE64'
 
-    container 'nf-core/sentieon:202112.06'
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' :
+        'biocontainers/sentieon:202308.01--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(vcf), path(vcf_tbi), path(recal), path(recal_index), path(tranches)
@@ -21,10 +24,15 @@ process SENTIEON_APPLYVARCAL {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: ''
@@ -47,6 +55,8 @@ process SENTIEON_APPLYVARCAL {
         echo "Decoded and exported Sentieon test-license system environment variables"
     fi
 
+    $fix_ld_library_path
+
     sentieon driver -r ${fasta}  --algo ApplyVarCal \\
         -v $vcf \\
         --recal $recal \\
@@ -61,12 +71,19 @@ process SENTIEON_APPLYVARCAL {
     """
 
     stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
+    $fix_ld_library_path
+
     touch ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
 
diff --git a/modules/nf-core/sentieon/bwamem/environment.yml b/modules/nf-core/sentieon/bwamem/environment.yml
index efd460cfa4..c090bfa5ae 100644
--- a/modules/nf-core/sentieon/bwamem/environment.yml
+++ b/modules/nf-core/sentieon/bwamem/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::sentieon=202308.01
diff --git a/modules/nf-core/sentieon/bwamem/main.nf b/modules/nf-core/sentieon/bwamem/main.nf
index b58835fda5..e25515e7eb 100644
--- a/modules/nf-core/sentieon/bwamem/main.nf
+++ b/modules/nf-core/sentieon/bwamem/main.nf
@@ -5,7 +5,10 @@ process SENTIEON_BWAMEM {
 
     secret 'SENTIEON_LICENSE_BASE64'
 
-    container 'nf-core/sentieon:202112.06'
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' :
+        'biocontainers/sentieon:202308.01--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(reads)
@@ -21,10 +24,15 @@ process SENTIEON_BWAMEM {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: ''
@@ -47,6 +55,8 @@ process SENTIEON_BWAMEM {
         echo "Decoded and exported Sentieon test-license system environment variables"
     fi
 
+    $fix_ld_library_path
+
     INDEX=`find -L ./ -name "*.amb" | sed 's/.amb//'`
 
     sentieon bwa mem \\
@@ -64,8 +74,19 @@ process SENTIEON_BWAMEM {
     """
 
     stub:
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
+    }
+
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
+    $fix_ld_library_path
+
     touch ${prefix}.bam
     touch ${prefix}.bam.bai
 
diff --git a/modules/nf-core/sentieon/dedup/environment.yml b/modules/nf-core/sentieon/dedup/environment.yml
index bda2b1385a..622cf73909 100644
--- a/modules/nf-core/sentieon/dedup/environment.yml
+++ b/modules/nf-core/sentieon/dedup/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::sentieon=202308.01
diff --git a/modules/nf-core/sentieon/dedup/main.nf b/modules/nf-core/sentieon/dedup/main.nf
index c83d5e552a..01ee885a17 100644
--- a/modules/nf-core/sentieon/dedup/main.nf
+++ b/modules/nf-core/sentieon/dedup/main.nf
@@ -5,7 +5,10 @@ process SENTIEON_DEDUP {
 
     secret 'SENTIEON_LICENSE_BASE64'
 
-    container 'nf-core/sentieon:202112.06'
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' :
+        'biocontainers/sentieon:202308.01--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai)
@@ -26,10 +29,15 @@ process SENTIEON_DEDUP {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def args = task.ext.args ?: ''
     def args2 = task.ext.args2 ?: ''
     def args3 = task.ext.args3 ?: ''
@@ -58,6 +66,8 @@ process SENTIEON_DEDUP {
         echo "Decoded and exported Sentieon test-license system environment variables"
     fi
 
+    $fix_ld_library_path
+
     sentieon driver $args $input_list -r ${fasta} --algo LocusCollector $args2 --fun score_info ${prefix}.score
     sentieon driver $args3 -t $task.cpus $input_list -r ${fasta} --algo Dedup $args4 --score_info ${prefix}.score --metrics ${metrics} ${prefix}${suffix}
     # This following tsv-file is produced in order to get a proper tsv-file with Dedup-metrics for importing in MultiQC as "custom content".
@@ -71,8 +81,19 @@ process SENTIEON_DEDUP {
     """
 
     stub:
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
+    }
+
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
+    $fix_ld_library_path
+
     touch ${prefix}.cram
     touch ${prefix}.cram.crai
     touch ${prefix}.metrics
diff --git a/modules/nf-core/sentieon/dnamodelapply/environment.yml b/modules/nf-core/sentieon/dnamodelapply/environment.yml
index 3f92a79ab8..6d27d44a1d 100644
--- a/modules/nf-core/sentieon/dnamodelapply/environment.yml
+++ b/modules/nf-core/sentieon/dnamodelapply/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::sentieon=202308.01
diff --git a/modules/nf-core/sentieon/dnamodelapply/main.nf b/modules/nf-core/sentieon/dnamodelapply/main.nf
index 3fe9a28f19..1cbb02e3b3 100644
--- a/modules/nf-core/sentieon/dnamodelapply/main.nf
+++ b/modules/nf-core/sentieon/dnamodelapply/main.nf
@@ -5,7 +5,10 @@ process SENTIEON_DNAMODELAPPLY {
 
     secret 'SENTIEON_LICENSE_BASE64'
 
-    container 'nf-core/sentieon:202112.06'
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' :
+        'biocontainers/sentieon:202308.01--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(vcf), path(idx)
@@ -22,10 +25,15 @@ process SENTIEON_DNAMODELAPPLY {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def args      = task.ext.args   ?: ''
     def prefix    = task.ext.prefix ?: "${meta.id}"
     def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: ''
@@ -48,6 +56,8 @@ process SENTIEON_DNAMODELAPPLY {
         echo "Decoded and exported Sentieon test-license system environment variables"
     fi
 
+    $fix_ld_library_path
+
     sentieon driver \\
         -t $task.cpus \\
         -r $fasta \\
@@ -64,12 +74,20 @@ process SENTIEON_DNAMODELAPPLY {
     """
 
     stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
     """
+    $fix_ld_library_path
+
     touch ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
 
diff --git a/modules/nf-core/sentieon/dnascope/environment.yml b/modules/nf-core/sentieon/dnascope/environment.yml
index 2c5b4937bf..45c2116c04 100644
--- a/modules/nf-core/sentieon/dnascope/environment.yml
+++ b/modules/nf-core/sentieon/dnascope/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::sentieon=202308.01
diff --git a/modules/nf-core/sentieon/dnascope/main.nf b/modules/nf-core/sentieon/dnascope/main.nf
index 6be42a1728..7e5adefc7c 100644
--- a/modules/nf-core/sentieon/dnascope/main.nf
+++ b/modules/nf-core/sentieon/dnascope/main.nf
@@ -5,7 +5,10 @@ process SENTIEON_DNASCOPE {
 
     secret 'SENTIEON_LICENSE_BASE64'
 
-    container 'nf-core/sentieon:202112.06'
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' :
+        'biocontainers/sentieon:202308.01--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(bam), path(bai), path(intervals)
@@ -29,10 +32,15 @@ process SENTIEON_DNASCOPE {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def args                      = task.ext.args                      ?: ''  // options for the driver
     def args2                     = task.ext.args2                     ?: ''  // options for the vcf generation
     def args3                     = task.ext.args3                     ?: ''  // options for the gvcf generation
@@ -72,6 +80,8 @@ process SENTIEON_DNASCOPE {
         echo "Decoded and exported Sentieon test-license system environment variables"
     fi
 
+    $fix_ld_library_path
+
     sentieon driver $args -r $fasta -t $task.cpus -i $bam $interval $vcf_cmd $gvcf_cmd
 
     cat <<-END_VERSIONS > versions.yml
@@ -81,12 +91,20 @@ process SENTIEON_DNASCOPE {
     """
 
     stub:
-    def prefix = task.ext.prefix ?: "${meta.id}"
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
+    def prefix = task.ext.prefix ?: "${meta.id}"
+
     """
+    $fix_ld_library_path
+
     touch ${prefix}.unfiltered.vcf.gz
     touch ${prefix}.unfiltered.vcf.gz.tbi
     touch ${prefix}.g.vcf.gz
diff --git a/modules/nf-core/sentieon/gvcftyper/environment.yml b/modules/nf-core/sentieon/gvcftyper/environment.yml
index 5af2aaa8e8..9a8143068a 100644
--- a/modules/nf-core/sentieon/gvcftyper/environment.yml
+++ b/modules/nf-core/sentieon/gvcftyper/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::sentieon=202308.01
diff --git a/modules/nf-core/sentieon/gvcftyper/main.nf b/modules/nf-core/sentieon/gvcftyper/main.nf
index 134faa6835..d2be759fe3 100644
--- a/modules/nf-core/sentieon/gvcftyper/main.nf
+++ b/modules/nf-core/sentieon/gvcftyper/main.nf
@@ -5,7 +5,10 @@ process SENTIEON_GVCFTYPER {
 
     secret 'SENTIEON_LICENSE_BASE64'
 
-    container 'nf-core/sentieon:202112.06'
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' :
+        'biocontainers/sentieon:202308.01--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(gvcfs), path(tbis), path(intervals)
@@ -23,10 +26,15 @@ process SENTIEON_GVCFTYPER {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def prefix = task.ext.prefix ?: "${meta.id}"
     def sentieon_auth_mech_base64 = task.ext.sentieon_auth_mech_base64 ?: ''
     def sentieon_auth_data_base64 = task.ext.sentieon_auth_data_base64 ?: ''
@@ -50,6 +58,8 @@ process SENTIEON_GVCFTYPER {
         echo "Decoded and exported Sentieon test-license system environment variables"
     fi
 
+    $fix_ld_library_path
+
     sentieon driver -r ${fasta} --algo GVCFtyper ${gvcfs_input} ${dbsnp_cmd} ${prefix}.vcf.gz
 
     cat <<-END_VERSIONS > versions.yml
@@ -59,13 +69,20 @@ process SENTIEON_GVCFTYPER {
     """
 
     stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def prefix = task.ext.prefix ?: "${meta.id}"
 
     """
+    $fix_ld_library_path
+
     touch ${prefix}.vcf.gz
     touch ${prefix}.vcf.gz.tbi
 
diff --git a/modules/nf-core/sentieon/haplotyper/environment.yml b/modules/nf-core/sentieon/haplotyper/environment.yml
index 5d8e4c58f9..a3a721cf1d 100644
--- a/modules/nf-core/sentieon/haplotyper/environment.yml
+++ b/modules/nf-core/sentieon/haplotyper/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::sentieon=202308.01
diff --git a/modules/nf-core/sentieon/haplotyper/main.nf b/modules/nf-core/sentieon/haplotyper/main.nf
index f83a137122..d349525dea 100644
--- a/modules/nf-core/sentieon/haplotyper/main.nf
+++ b/modules/nf-core/sentieon/haplotyper/main.nf
@@ -5,7 +5,10 @@ process SENTIEON_HAPLOTYPER {
 
     secret 'SENTIEON_LICENSE_BASE64'
 
-    container 'nf-core/sentieon:202112.06'
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' :
+        'biocontainers/sentieon:202308.01--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(input), path(input_index), path(intervals)
@@ -27,10 +30,15 @@ process SENTIEON_HAPLOTYPER {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def args = task.ext.args ?: ''    // options for the driver
     def args2 = task.ext.args2 ?: ''  // options for the vcf generation
     def args3 = task.ext.args3 ?: ''  // options for the gvcf generation
@@ -68,6 +76,8 @@ process SENTIEON_HAPLOTYPER {
         echo "Decoded and exported Sentieon test-license system environment variables"
     fi
 
+    $fix_ld_library_path
+
     sentieon driver $args -r $fasta -t $task.cpus -i $input $interval_command $vcf_cmd $gvcf_cmd
 
     cat <<-END_VERSIONS > versions.yml
@@ -77,8 +87,19 @@ process SENTIEON_HAPLOTYPER {
     """
 
     stub:
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
+    }
+
     def prefix = task.ext.prefix ?: "${meta.id}"
     """
+    $fix_ld_library_path
+
     touch ${prefix}.unfiltered.vcf.gz
     touch ${prefix}.unfiltered.vcf.gz.tbi
     touch ${prefix}.g.vcf.gz
diff --git a/modules/nf-core/sentieon/varcal/environment.yml b/modules/nf-core/sentieon/varcal/environment.yml
index f04a8b78ce..93921ff046 100644
--- a/modules/nf-core/sentieon/varcal/environment.yml
+++ b/modules/nf-core/sentieon/varcal/environment.yml
@@ -3,3 +3,5 @@ channels:
   - conda-forge
   - bioconda
   - defaults
+dependencies:
+  - bioconda::sentieon=202308.01
diff --git a/modules/nf-core/sentieon/varcal/main.nf b/modules/nf-core/sentieon/varcal/main.nf
index 41e0ea40f5..7a0c807127 100644
--- a/modules/nf-core/sentieon/varcal/main.nf
+++ b/modules/nf-core/sentieon/varcal/main.nf
@@ -5,7 +5,10 @@ process SENTIEON_VARCAL {
 
     secret 'SENTIEON_LICENSE_BASE64'
 
-    container 'nf-core/sentieon:202112.06'
+    conda "${moduleDir}/environment.yml"
+    container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ?
+        'https://depot.galaxyproject.org/singularity/sentieon:202308.01--h43eeafb_0' :
+        'biocontainers/sentieon:202308.01--h43eeafb_0' }"
 
     input:
     tuple val(meta), path(vcf), path(tbi) // input vcf and tbi of variants to recalibrate
@@ -26,10 +29,15 @@ process SENTIEON_VARCAL {
     task.ext.when == null || task.ext.when
 
     script:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def args = task.ext.args ?: ''
     def prefix = task.ext.prefix ?: "${meta.id}"
     def reference_command = fasta ? "--reference $fasta " : ''
@@ -69,6 +77,8 @@ process SENTIEON_VARCAL {
         echo "Decoded and exported Sentieon test-license system environment variables"
     fi
 
+    $fix_ld_library_path
+
     sentieon driver -r ${fasta}  --algo VarCal \\
         -v $vcf \\
         --tranches_file ${prefix}.tranches \\
@@ -83,12 +93,19 @@ process SENTIEON_VARCAL {
     """
 
     stub:
-    // Exit if running this module with -profile conda / -profile mamba
-    if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) {
-        error "Sentieon modules do not support Conda. Please use Docker / Singularity / Podman instead."
+    // The following code sets LD_LIBRARY_PATH in the script-section when the module is run by Singularity.
+    // That turned out to be one way of overcoming the following issue with the Singularity-Sentieon-containers from galaxy, Sentieon (LD_LIBRARY_PATH) and the way Nextflow runs Singularity-containers.
+    // The galaxy container uses a runscript which is responsible for setting LD_PRELOAD properly. Nextflow executes singularity containers using `singularity exec`, which avoids the run script, leading to the LD_LIBRARY_PATH/libstdc++.so.6 error.
+    if (workflow.containerEngine == 'singularity') {
+        fix_ld_library_path = 'LD_LIBRARY_PATH=/usr/local/lib/:\$LD_LIBRARY_PATH;export LD_LIBRARY_PATH'
+    } else {
+        fix_ld_library_path = ''
     }
+
     def prefix   = task.ext.prefix ?: "${meta.id}"
     """
+    $fix_ld_library_path
+
     touch ${prefix}.recal
     touch ${prefix}.idx
     touch ${prefix}.tranches
diff --git a/tests/test_sentieon_dnascope.yml b/tests/test_sentieon_dnascope.yml
index f51e0bca72..946bed2d4f 100644
--- a/tests/test_sentieon_dnascope.yml
+++ b/tests/test_sentieon_dnascope.yml
@@ -17,7 +17,7 @@
     - path: results/preprocessing/recalibrated/test/test.recal.cram.crai
       should_exist: false
     - path: results/reports/bcftools/sentieon_dnascope/test/test.dnascope.filtered.bcftools_stats.txt
-      md5sum: 912c7d5b31784c50e0a75b4fcfa4997b
+      md5sum: fb3923060b59b7dc18705cac5704caba
     - path: results/reports/vcftools/sentieon_dnascope/test/test.dnascope.filtered.FILTER.summary
       md5sum: e67b24d296810a075378e5864bcea0fa
     - path: results/reports/vcftools/sentieon_dnascope/test/test.dnascope.filtered.TsTv.count
@@ -60,7 +60,7 @@
     - path: results/preprocessing/recalibrated/test/test.recal.cram.crai
       should_exist: false
     - path: results/reports/bcftools/sentieon_dnascope/test/test.dnascope.filtered.bcftools_stats.txt
-      md5sum: 912c7d5b31784c50e0a75b4fcfa4997b
+      md5sum: fb3923060b59b7dc18705cac5704caba
     - path: results/reports/vcftools/sentieon_dnascope/test/test.dnascope.filtered.FILTER.summary
       md5sum: e67b24d296810a075378e5864bcea0fa
     - path: results/reports/vcftools/sentieon_dnascope/test/test.dnascope.filtered.TsTv.count