From 739fbb55578267c3260e307bb3b29a7fedd5822e Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Fri, 19 Jan 2024 11:40:02 +0000 Subject: [PATCH 001/111] add nf-test for alevin aligner --- .gitignore | 1 + nf-test.config | 8 ++++ tests/main_pipeline_alevin.test | 68 ++++++++++++++++++++++++++++ tests/main_pipeline_alevin.test.snap | 32 +++++++++++++ tests/nextflow.config | 32 +++++++++++++ 5 files changed, 141 insertions(+) create mode 100644 nf-test.config create mode 100644 tests/main_pipeline_alevin.test create mode 100644 tests/main_pipeline_alevin.test.snap create mode 100644 tests/nextflow.config diff --git a/.gitignore b/.gitignore index 5d402163..a9ba2c52 100644 --- a/.gitignore +++ b/.gitignore @@ -9,3 +9,4 @@ testing* log/ reports/ testme.sh +.nf-test/ diff --git a/nf-test.config b/nf-test.config new file mode 100644 index 00000000..2fa82adf --- /dev/null +++ b/nf-test.config @@ -0,0 +1,8 @@ +config { + + testsDir "tests" + workDir ".nf-test" + configFile "tests/nextflow.config" + profile "docker" + +} diff --git a/tests/main_pipeline_alevin.test b/tests/main_pipeline_alevin.test new file mode 100644 index 00000000..c51a8ee9 --- /dev/null +++ b/tests/main_pipeline_alevin.test @@ -0,0 +1,68 @@ +nextflow_pipeline { + + name "Test Workflow main.nf" + script "main.nf" + + test("test-dataset_alevin_aligner") { + + when { + // the rest is taken from shared config + params { + aligner = 'alevin' + outdir = "${outputDir}/results_alevin" + + // Limit resources so that this can run on GitHub Actions -- for some reason it had not been taken from shared config + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + } + } + + then { + + // + // General assertions + // + + // Did it finish successfully? + assert workflow.success + + // How many tasks were executed? + assert workflow.trace.tasks().size() == 16 + + // How many results were produced? + assert path("${outputDir}/results_alevin").list().size() == 5 + assert path("${outputDir}/results_alevin/alevin").list().size() == 4 + assert path("${outputDir}/results_alevin/alevin/mtx_conversions").list().size() == 4 + assert path("${outputDir}/results_alevin/alevinqc").list().size() == 2 + assert path("${outputDir}/results_alevin/fastqc").list().size() == 12 + assert path("${outputDir}/results_alevin/multiqc").list().size() == 3 + + // + // Check if files were produced + // + assert new File( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_cols.txt" ).exists() + assert new File( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat.mtx" ).exists() + assert new File( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_rows.txt" ).exists() + + assert new File( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_cols.txt" ).exists() + assert new File( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat.mtx" ).exists() + assert new File( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_rows.txt" ).exists() + + // + // Check if files are the same + // + assert snapshot( + workflow, + path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_cols.txt" ), + path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat.mtx" ), + path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_rows.txt" ), + path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_cols.txt" ), + path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat.mtx" ), + path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_rows.txt" ) + ).match() + + } + } + +} diff --git a/tests/main_pipeline_alevin.test.snap b/tests/main_pipeline_alevin.test.snap new file mode 100644 index 00000000..9d194012 --- /dev/null +++ b/tests/main_pipeline_alevin.test.snap @@ -0,0 +1,32 @@ +{ + "test-dataset_alevin_aligner": { + "content": [ + { + "stderr": [ + + ], + "errorReport": "", + "exitStatus": 0, + "failed": false, + "stdout": [ + + ], + "errorMessage": "", + "trace": { + "tasksFailed": 0, + "tasksCount": 16, + "tasksSucceeded": 16 + }, + "name": "workflow", + "success": true + }, + "quants_mat_cols.txt:md5,e9868982c17a330392e38c2a5933cf97", + "quants_mat.mtx:md5,b8aa7b3c488fd8923de50a3621d4991f", + "quants_mat_rows.txt:md5,6227df5a13127b71c71fb18cd8574857", + "quants_mat_cols.txt:md5,e9868982c17a330392e38c2a5933cf97", + "quants_mat.mtx:md5,54cd12666016adce94c025b2e07f4b02", + "quants_mat_rows.txt:md5,6b458a7777260ba90eccbe7919df934b" + ], + "timestamp": "2024-01-19T10:28:35.652763852" + } +} \ No newline at end of file diff --git a/tests/nextflow.config b/tests/nextflow.config new file mode 100644 index 00000000..66e56d29 --- /dev/null +++ b/tests/nextflow.config @@ -0,0 +1,32 @@ +/* +======================================================================================== + Nextflow config file for running tests +======================================================================================== +*/ + +// Copy from test.config for standardization +params { + config_profile_name = 'Test profile' + config_profile_description = 'Minimal test dataset to check pipeline function' + + // Limit resources so that this can run on GitHub Actions + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + + // Input data + input = 'https://github.com/nf-core/test-datasets/raw/scrnaseq/samplesheet-2-0.csv' + + // Genome references + fasta = 'https://github.com/nf-core/test-datasets/raw/scrnaseq/reference/GRCm38.p6.genome.chr19.fa' + gtf = 'https://github.com/nf-core/test-datasets/raw/scrnaseq/reference/gencode.vM19.annotation.chr19.gtf' + protocol = '10XV2' + + validationSchemaIgnoreParams = 'genomes' +} + +process { + withName: '.*:CELLRANGER_COUNT' { + maxForks = 1 + } +} From 23072dc4271f7b4461426fbe0a226d3e190d2b4a Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Fri, 19 Jan 2024 15:51:42 +0000 Subject: [PATCH 002/111] adding nf-test for star aligner --- tests/main_pipeline_star.test | 87 ++++++++++++++++++++++++++++++ tests/main_pipeline_star.test.snap | 38 +++++++++++++ 2 files changed, 125 insertions(+) create mode 100644 tests/main_pipeline_star.test create mode 100644 tests/main_pipeline_star.test.snap diff --git a/tests/main_pipeline_star.test b/tests/main_pipeline_star.test new file mode 100644 index 00000000..d20657f1 --- /dev/null +++ b/tests/main_pipeline_star.test @@ -0,0 +1,87 @@ +nextflow_pipeline { + + name "Test Workflow main.nf" + script "main.nf" + + test("test-dataset_star_aligner") { + + when { + // the rest is taken from shared config + params { + aligner = 'star' + outdir = "${outputDir}/results_star" + + // Limit resources so that this can run on GitHub Actions -- for some reason it had not been taken from shared config + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + } + } + + then { + + // + // General assertions + // + + // Did it finish successfully? + assert workflow.success + + // How many tasks were executed? + assert workflow.trace.tasks().size() == 14 + + // How many results were produced? + assert path("${outputDir}/results_star").list().size() == 4 + assert path("${outputDir}/results_star/star").list().size() == 3 + assert path("${outputDir}/results_star/star/mtx_conversions").list().size() == 4 + assert path("${outputDir}/results_star/fastqc").list().size() == 12 + assert path("${outputDir}/results_star/multiqc").list().size() == 3 + + // + // Check if files were produced + // + // + // Check if main files exist + // + + assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.SJ.out.tab" ).exists() + assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Barcodes.stats" ).exists() + assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/matrix.mtx.gz" ).exists() + assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/features.tsv.gz" ).exists() + assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/barcodes.tsv.gz" ).exists() + assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() + assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.rds" ).exists() + + assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.SJ.out.tab" ).exists() + assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Barcodes.stats" ).exists() + assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/matrix.mtx.gz" ).exists() + assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/features.tsv.gz" ).exists() + assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/barcodes.tsv.gz" ).exists() + assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() + assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ).exists() + + // + // Check if files are the same + // + assert snapshot( + workflow, + path( "${outputDir}/results_star/star/Sample_X/Sample_X.SJ.out.tab" ), + path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Barcodes.stats" ), + path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/matrix.mtx.gz" ), + path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/features.tsv.gz" ), + path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/barcodes.tsv.gz" ), + // path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ), // does not match + path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.SJ.out.tab" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Barcodes.stats" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/matrix.mtx.gz" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/features.tsv.gz" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/barcodes.tsv.gz" ), + // path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ), // does not match + path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ), + ).match() + + } + } + +} diff --git a/tests/main_pipeline_star.test.snap b/tests/main_pipeline_star.test.snap new file mode 100644 index 00000000..ef086f60 --- /dev/null +++ b/tests/main_pipeline_star.test.snap @@ -0,0 +1,38 @@ +{ + "test-dataset_star_aligner": { + "content": [ + { + "stderr": [ + + ], + "errorReport": "", + "exitStatus": 0, + "failed": false, + "stdout": [ + + ], + "errorMessage": "", + "trace": { + "tasksFailed": 0, + "tasksCount": 14, + "tasksSucceeded": 14 + }, + "name": "workflow", + "success": true + }, + "Sample_X.SJ.out.tab:md5,d2d7f0abe38029012571bdf6622fc6eb", + "Barcodes.stats:md5,7f99dc8aa5e074fbe5779ea7712c0886", + "matrix.mtx.gz:md5,6a923393343aa1a69b0cf1bd998c9285", + "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", + "barcodes.tsv.gz:md5,9a7dacaa1779ea43c1507a947fe6992a", + "Sample_X_matrix.rds:md5,aa2d36dd8507aba864347c88e4ce0d27", + "Sample_Y.SJ.out.tab:md5,98bd31104a860cf80119dc30d938d163", + "Barcodes.stats:md5,2dbf1ae426c1afd97903ee001f0db5ce", + "matrix.mtx.gz:md5,0ae080bd0002e350531a5816e159345e", + "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", + "barcodes.tsv.gz:md5,9b695b0b91bcb146ec9c4688ca10a690", + "Sample_Y_matrix.rds:md5,d459af8f99258bcc88b80b2f7c58e911" + ], + "timestamp": "2024-01-19T15:46:22.470527538" + } +} \ No newline at end of file From 19132fff4a12861e0c68b6aa959613329dafd271 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 22 Jan 2024 13:37:45 -0800 Subject: [PATCH 003/111] first commit: bump up kb versions --- modules/nf-core/kallistobustools/count/environment.yml | 2 +- modules/nf-core/kallistobustools/count/main.nf | 4 ++-- modules/nf-core/kallistobustools/ref/environment.yml | 2 +- modules/nf-core/kallistobustools/ref/main.nf | 4 ++-- 4 files changed, 6 insertions(+), 6 deletions(-) diff --git a/modules/nf-core/kallistobustools/count/environment.yml b/modules/nf-core/kallistobustools/count/environment.yml index 7ff8a2da..024f0afc 100644 --- a/modules/nf-core/kallistobustools/count/environment.yml +++ b/modules/nf-core/kallistobustools/count/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::kb-python=0.27.2 + - bioconda::kb-python=0.28.2 diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index 036bb35d..bfd9a625 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -4,8 +4,8 @@ process KALLISTOBUSTOOLS_COUNT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/kb-python:0.27.2--pyhdfd78af_0' : - 'biocontainers/kb-python:0.27.2--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/kb-python:0.28.2--pyhdfd78af_0' : + 'biocontainers/kb-python:0.28.2--pyhdfd78af_0' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/kallistobustools/ref/environment.yml b/modules/nf-core/kallistobustools/ref/environment.yml index acbd0e0a..6ae07a8c 100644 --- a/modules/nf-core/kallistobustools/ref/environment.yml +++ b/modules/nf-core/kallistobustools/ref/environment.yml @@ -4,5 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::kb-python=0.27.2 + - bioconda::kb-python=0.28.2 - conda-forge::requests>=2.23.0 diff --git a/modules/nf-core/kallistobustools/ref/main.nf b/modules/nf-core/kallistobustools/ref/main.nf index 68d72ca9..759e8663 100644 --- a/modules/nf-core/kallistobustools/ref/main.nf +++ b/modules/nf-core/kallistobustools/ref/main.nf @@ -4,8 +4,8 @@ process KALLISTOBUSTOOLS_REF { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/kb-python:0.27.2--pyhdfd78af_0' : - 'biocontainers/kb-python:0.27.2--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/kb-python:0.28.2--pyhdfd78af_0' : + 'biocontainers/kb-python:0.28.2--pyhdfd78af_0' }" input: path fasta From 8037005f0e2bcca40a86164cb3b6ec38a93c3a85 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Tue, 23 Jan 2024 09:13:43 +0100 Subject: [PATCH 004/111] Bump version to 2.6.0dev --- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 10ec1af4..051ed54e 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/scrnaseq + This report has been generated by the nf-core/scrnaseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-scrnaseq-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 9e83c42a..7af20f8c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -272,7 +272,7 @@ manifest { description = """Pipeline for processing 10x Genomics single cell rnaseq data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.5.1' + version = '2.6.0dev' doi = '10.5281/zenodo.3568187' } From 4ae28e384a4e28fa2e4e5bc8bfe3536c4b1d407a Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 23 Jan 2024 11:51:02 +0000 Subject: [PATCH 005/111] add nf-tests for cellranger aligner --- tests/main_pipeline_cellranger.test | 84 ++++++++++++++++++++++++ tests/main_pipeline_cellranger.test.snap | 40 +++++++++++ 2 files changed, 124 insertions(+) create mode 100644 tests/main_pipeline_cellranger.test create mode 100644 tests/main_pipeline_cellranger.test.snap diff --git a/tests/main_pipeline_cellranger.test b/tests/main_pipeline_cellranger.test new file mode 100644 index 00000000..c90436f6 --- /dev/null +++ b/tests/main_pipeline_cellranger.test @@ -0,0 +1,84 @@ +nextflow_pipeline { + + name "Test Workflow main.nf" + script "main.nf" + + test("test-dataset_cellranger_aligner") { + + when { + // the rest is taken from shared config + params { + aligner = 'cellranger' + outdir = "${outputDir}/results_cellranger" + + // Limit resources so that this can run on GitHub Actions -- for some reason it had not been taken from shared config + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + } + } + + then { + + // + // General assertions + // + + // Did it finish successfully? + assert workflow.success + + // How many tasks were executed? + assert workflow.trace.tasks().size() == 15 + + // How many results were produced? + assert path("${outputDir}/results_cellranger").list().size() == 4 + assert path("${outputDir}/results_cellranger/cellranger").list().size() == 4 + assert path("${outputDir}/results_cellranger/cellranger/mtx_conversions").list().size() == 4 + assert path("${outputDir}/results_cellranger/cellranger/count").list().size() == 3 + assert path("${outputDir}/results_cellranger/fastqc").list().size() == 12 + assert path("${outputDir}/results_cellranger/multiqc").list().size() == 3 + + // + // Check if files were produced + // + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/features.tsv.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ).exists() + + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/features.tsv.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ).exists() + + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/features.tsv.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/matrix.mtx.gz" ).exists() + + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/features.tsv.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/matrix.mtx.gz" ).exists() + + // + // Check if files are the same + // + assert snapshot( + workflow, + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/features.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/features.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/features.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/matrix.mtx.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/features.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/matrix.mtx.gz" ), + path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), + path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ) + ).match() + + } + } + +} diff --git a/tests/main_pipeline_cellranger.test.snap b/tests/main_pipeline_cellranger.test.snap new file mode 100644 index 00000000..5f88b954 --- /dev/null +++ b/tests/main_pipeline_cellranger.test.snap @@ -0,0 +1,40 @@ +{ + "test-dataset_cellranger_aligner": { + "content": [ + { + "stderr": [ + + ], + "errorReport": "", + "exitStatus": 0, + "failed": false, + "stdout": [ + + ], + "errorMessage": "", + "trace": { + "tasksFailed": 0, + "tasksCount": 15, + "tasksSucceeded": 15 + }, + "name": "workflow", + "success": true + }, + "barcodes.tsv.gz:md5,fe6e51564b4405b37ca8604a844b1f2e", + "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", + "matrix.mtx.gz:md5,79471f700ec5bab852a960f0d1537705", + "barcodes.tsv.gz:md5,77afe9a76631fc7b44236d3962a55aa5", + "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", + "matrix.mtx.gz:md5,e224aa759250fa5730dc069bce9be253", + "barcodes.tsv.gz:md5,85da6b6e0c78dfe81af8c07c2017ab5e", + "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", + "matrix.mtx.gz:md5,1bc71041a425bfcee38472bfec5997f8", + "barcodes.tsv.gz:md5,081f72b5252ccaf5ffd535ffbd235c4c", + "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", + "matrix.mtx.gz:md5,a4db04e43e650accc96361a287126a6b", + "Sample_X_matrix.rds:md5,f9191ba575a3ab79ada4807715f18573", + "Sample_Y_matrix.rds:md5,7be3f7b29d668dcf7e951b9f4d371a5e" + ], + "timestamp": "2024-01-22T15:19:20.134275449" + } +} \ No newline at end of file From b56577ce413a2a93ecc950b85bd1a7e0e235e38f Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 23 Jan 2024 12:25:46 +0000 Subject: [PATCH 006/111] add nf-test for kallisto aligner --- tests/main_pipeline_kallisto.test | 71 ++++++++++++++++++++++++++ tests/main_pipeline_kallisto.test.snap | 34 ++++++++++++ 2 files changed, 105 insertions(+) create mode 100644 tests/main_pipeline_kallisto.test create mode 100644 tests/main_pipeline_kallisto.test.snap diff --git a/tests/main_pipeline_kallisto.test b/tests/main_pipeline_kallisto.test new file mode 100644 index 00000000..3bae1f31 --- /dev/null +++ b/tests/main_pipeline_kallisto.test @@ -0,0 +1,71 @@ +nextflow_pipeline { + + name "Test Workflow main.nf" + script "main.nf" + + test("test-dataset_kallisto_aligner") { + + when { + // the rest is taken from shared config + params { + aligner = 'kallisto' + outdir = "${outputDir}/results_kallisto" + + // Limit resources so that this can run on GitHub Actions -- for some reason it had not been taken from shared config + max_cpus = 2 + max_memory = '6.GB' + max_time = '6.h' + } + } + + then { + + // + // General assertions + // + + // Did it finish successfully? + assert workflow.success + + // How many tasks were executed? + assert workflow.trace.tasks().size() == 14 + + // How many results were produced? + assert path("${outputDir}/results_kallisto").list().size() == 4 + assert path("${outputDir}/results_kallisto/kallisto").list().size() == 4 + assert path("${outputDir}/results_kallisto/kallisto/mtx_conversions").list().size() == 4 + assert path("${outputDir}/results_kallisto/kallisto/Sample_X.count").list().size() == 9 + assert path("${outputDir}/results_kallisto/kallisto/Sample_Y.count").list().size() == 9 + assert path("${outputDir}/results_kallisto/fastqc").list().size() == 12 + assert path("${outputDir}/results_kallisto/multiqc").list().size() == 3 + + // + // Check if files were produced + // + assert new File( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.barcodes.txt" ).exists() + assert new File( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.genes.txt" ).exists() + assert new File( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.mtx" ).exists() + + assert new File( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.barcodes.txt" ).exists() + assert new File( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.genes.txt" ).exists() + assert new File( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.mtx" ).exists() + + // + // Check if files are the same + // + assert snapshot( + workflow, + path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.barcodes.txt" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.genes.txt" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.mtx" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.barcodes.txt" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.genes.txt" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.mtx" ), + path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), + path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ) + ).match() + + } + } + +} diff --git a/tests/main_pipeline_kallisto.test.snap b/tests/main_pipeline_kallisto.test.snap new file mode 100644 index 00000000..b0fb50bb --- /dev/null +++ b/tests/main_pipeline_kallisto.test.snap @@ -0,0 +1,34 @@ +{ + "test-dataset_kallisto_aligner": { + "content": [ + { + "stderr": [ + + ], + "errorReport": "", + "exitStatus": 0, + "failed": false, + "stdout": [ + + ], + "errorMessage": "", + "trace": { + "tasksFailed": 0, + "tasksCount": 14, + "tasksSucceeded": 14 + }, + "name": "workflow", + "success": true + }, + "cells_x_genes.barcodes.txt:md5,18be561873e435d4587f6b3f95a0e301", + "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", + "cells_x_genes.mtx:md5,37d2cd8c712f9c70463e87485bf6cd36", + "cells_x_genes.barcodes.txt:md5,488437e1f5477243697efb93366e5676", + "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", + "cells_x_genes.mtx:md5,af90e05b404490f6cb133ab7f62949f8", + "Sample_X_matrix.rds:md5,f0e43f69403f4b2e7704065421592ad0", + "Sample_Y_matrix.rds:md5,61809156e64dbdaf254cbc1c3456588e" + ], + "timestamp": "2024-01-23T12:19:47.921508953" + } +} \ No newline at end of file From 7a01c5b1df653b80ce48380bc55ca850c945524b Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 29 Jan 2024 15:13:19 +0000 Subject: [PATCH 007/111] Template update for nf-core/tools version 2.12 --- .editorconfig | 8 + .github/workflows/awsfulltest.yml | 2 +- .github/workflows/awstest.yml | 2 +- .github/workflows/branch.yml | 2 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 67 +++++ .github/workflows/fix-linting.yml | 72 +++-- .github/workflows/linting.yml | 61 +--- .github/workflows/linting_comment.yml | 2 +- .github/workflows/release-announcements.yml | 4 +- .gitpod.yml | 1 + .pre-commit-config.yaml | 7 +- CHANGELOG.md | 2 +- README.md | 8 +- assets/email_template.html | 2 +- assets/email_template.txt | 2 +- assets/nf-core-scrnaseq_logo_light.png | Bin 75757 -> 79070 bytes docs/images/nf-core-scrnaseq_logo_dark.png | Bin 75869 -> 29605 bytes docs/images/nf-core-scrnaseq_logo_light.png | Bin 75757 -> 25501 bytes lib/WorkflowMain.groovy | 16 +- lib/nfcore_external_java_deps.jar | Bin 2291171 -> 0 bytes main.nf | 2 +- modules.json | 6 +- .../dumpsoftwareversions/environment.yml | 2 +- .../custom/dumpsoftwareversions/main.nf | 4 +- .../dumpsoftwareversions/tests/main.nf.test | 7 +- .../tests/main.nf.test.snap | 50 ++-- modules/nf-core/fastqc/tests/main.nf.test | 265 ++++++++++++------ .../nf-core/fastqc/tests/main.nf.test.snap | 12 +- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 6 +- modules/nf-core/multiqc/meta.yml | 1 - modules/nf-core/multiqc/tests/main.nf.test | 48 +++- .../nf-core/multiqc/tests/main.nf.test.snap | 21 ++ nextflow.config | 5 +- pyproject.toml | 17 +- workflows/scrnaseq.nf | 7 + 37 files changed, 492 insertions(+), 223 deletions(-) create mode 100644 .github/workflows/download_pipeline.yml delete mode 100644 lib/nfcore_external_java_deps.jar create mode 100644 modules/nf-core/multiqc/tests/main.nf.test.snap diff --git a/.editorconfig b/.editorconfig index b6b31907..9b990088 100644 --- a/.editorconfig +++ b/.editorconfig @@ -22,3 +22,11 @@ indent_size = unset [/assets/email*] indent_size = unset + +# ignore Readme +[README.md] +indent_style = unset + +# ignore python +[*.{py}] +indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 65b078e4..33ee6733 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 060785d7..edc7b3a3 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v3 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index ae5b5d4b..68083c82 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v1 + uses: mshick/add-pr-comment@v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index 694e90ec..e37cfda5 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v7 + - uses: actions/stale@v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml new file mode 100644 index 00000000..8611458a --- /dev/null +++ b/.github/workflows/download_pipeline.yml @@ -0,0 +1,67 @@ +name: Test successful pipeline download with 'nf-core download' + +# Run the workflow when: +# - dispatched manually +# - when a PR is opened or reopened to master branch +# - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. +on: + workflow_dispatch: + pull_request: + types: + - opened + branches: + - master + pull_request_target: + branches: + - master + +env: + NXF_ANSI_LOG: false + +jobs: + download: + runs-on: ubuntu-latest + steps: + - name: Install Nextflow + uses: nf-core/setup-nextflow@v1 + + - uses: actions/setup-python@v5 + with: + python-version: "3.11" + architecture: "x64" + - uses: eWaterCycle/setup-singularity@v7 + with: + singularity-version: 3.8.3 + + - name: Install dependencies + run: | + python -m pip install --upgrade pip + pip install git+https://github.com/nf-core/tools.git@dev + + - name: Get the repository name and current branch set as environment variable + run: | + echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} + echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + + - name: Download the pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + run: | + nf-core download ${{ env.REPO_LOWERCASE }} \ + --revision ${{ env.REPO_BRANCH }} \ + --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ + --compress "none" \ + --container-system 'singularity' \ + --container-library "quay.io" -l "docker.io" -l "ghcr.io" \ + --container-cache-utilisation 'amend' \ + --download-configuration + + - name: Inspect download + run: tree ./${{ env.REPOTITLE_LOWERCASE }} + + - name: Run the downloaded pipeline + env: + NXF_SINGULARITY_CACHEDIR: ./ + NXF_SINGULARITY_HOME_MOUNT: true + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/fix-linting.yml b/.github/workflows/fix-linting.yml index 05b700e4..76f5e5bd 100644 --- a/.github/workflows/fix-linting.yml +++ b/.github/workflows/fix-linting.yml @@ -4,7 +4,7 @@ on: types: [created] jobs: - deploy: + fix-linting: # Only run if comment is on a PR with the main repo, and if it contains the magic keywords if: > contains(github.event.comment.html_url, '/pull/') && @@ -13,10 +13,17 @@ jobs: runs-on: ubuntu-latest steps: # Use the @nf-core-bot token to check out so we can push later - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 with: token: ${{ secrets.nf_core_bot_auth_token }} + # indication that the linting is being fixed + - name: React on comment + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: eyes + # Action runs on the issue comment, so we don't get the PR by default # Use the gh cli to check out the PR - name: Checkout Pull Request @@ -24,32 +31,59 @@ jobs: env: GITHUB_TOKEN: ${{ secrets.nf_core_bot_auth_token }} - - uses: actions/setup-node@v4 + # Install and run pre-commit + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 + with: + python-version: 3.11 - - name: Install Prettier - run: npm install -g prettier @prettier/plugin-php + - name: Install pre-commit + run: pip install pre-commit - # Check that we actually need to fix something - - name: Run 'prettier --check' - id: prettier_status - run: | - if prettier --check ${GITHUB_WORKSPACE}; then - echo "result=pass" >> $GITHUB_OUTPUT - else - echo "result=fail" >> $GITHUB_OUTPUT - fi + - name: Run pre-commit + id: pre-commit + run: pre-commit run --all-files + continue-on-error: true - - name: Run 'prettier --write' - if: steps.prettier_status.outputs.result == 'fail' - run: prettier --write ${GITHUB_WORKSPACE} + # indication that the linting has finished + - name: react if linting finished succesfully + if: steps.pre-commit.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: "+1" - name: Commit & push changes - if: steps.prettier_status.outputs.result == 'fail' + id: commit-and-push + if: steps.pre-commit.outcome == 'failure' run: | git config user.email "core@nf-co.re" git config user.name "nf-core-bot" git config push.default upstream git add . git status - git commit -m "[automated] Fix linting with Prettier" + git commit -m "[automated] Fix code linting" git push + + - name: react if linting errors were fixed + id: react-if-fixed + if: steps.commit-and-push.outcome == 'success' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: hooray + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + comment-id: ${{ github.event.comment.id }} + reactions: confused + + - name: react if linting errors were not fixed + if: steps.commit-and-push.outcome == 'failure' + uses: peter-evans/create-or-update-comment@71345be0265236311c031f5c7866368bd1eff043 # v4 + with: + issue-number: ${{ github.event.issue.number }} + body: | + @${{ github.actor }} I tried to fix the linting errors, but it didn't work. Please fix them manually. + See [CI log](https://github.com/nf-core/scrnaseq/actions/runs/${{ github.run_id }}) for more details. diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 905c58e4..81cd098e 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -11,61 +11,22 @@ on: types: [published] jobs: - EditorConfig: + pre-commit: runs-on: ubuntu-latest steps: - uses: actions/checkout@v4 - - uses: actions/setup-node@v4 - - - name: Install editorconfig-checker - run: npm install -g editorconfig-checker - - - name: Run ECLint check - run: editorconfig-checker -exclude README.md $(find .* -type f | grep -v '.git\|.py\|.md\|json\|yml\|yaml\|html\|css\|work\|.nextflow\|build\|nf_core.egg-info\|log.txt\|Makefile') - - Prettier: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - uses: actions/setup-node@v4 - - - name: Install Prettier - run: npm install -g prettier - - - name: Run Prettier --check - run: prettier --check ${GITHUB_WORKSPACE} - - PythonBlack: - runs-on: ubuntu-latest - steps: - - uses: actions/checkout@v4 - - - name: Check code lints with Black - uses: psf/black@stable - - # If the above check failed, post a comment on the PR explaining the failure - - name: Post PR comment - if: failure() - uses: mshick/add-pr-comment@v1 + - name: Set up Python 3.11 + uses: actions/setup-python@v5 with: - message: | - ## Python linting (`black`) is failing - - To keep the code consistent with lots of contributors, we run automated code consistency checks. - To fix this CI test, please run: - - * Install [`black`](https://black.readthedocs.io/en/stable/): `pip install black` - * Fix formatting errors in your pipeline: `black .` - - Once you push these changes the test should pass, and you can hide this comment :+1: + python-version: 3.11 + cache: "pip" - We highly recommend setting up Black in your code editor so that this formatting is done automatically on save. Ask about it on Slack for help! + - name: Install pre-commit + run: pip install pre-commit - Thanks again for your contribution! - repo-token: ${{ secrets.GITHUB_TOKEN }} - allow-repeats: false + - name: Run pre-commit + run: pre-commit run --all-files nf-core: runs-on: ubuntu-latest @@ -76,7 +37,7 @@ jobs: - name: Install Nextflow uses: nf-core/setup-nextflow@v1 - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.11" architecture: "x64" @@ -99,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v3 + uses: actions/upload-artifact@v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 0bbcd30f..147bcd10 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v2 + uses: dawidd6/action-download-artifact@v3 with: workflow: linting.yml workflow_conclusion: completed diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 6ad33927..21ac3f06 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -24,7 +24,7 @@ jobs: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v4 + - uses: actions/setup-python@v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +56,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.0.2 + - uses: zentered/bluesky-post-action@v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/.gitpod.yml b/.gitpod.yml index acf72695..363d5b1d 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -7,6 +7,7 @@ tasks: - name: unset JAVA_TOOL_OPTIONS command: | unset JAVA_TOOL_OPTIONS + vscode: extensions: # based on nf-core.nf-core-extensionpack - codezombiech.gitignore # Language support for .gitignore files diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml index 0c31cdb9..af57081f 100644 --- a/.pre-commit-config.yaml +++ b/.pre-commit-config.yaml @@ -1,5 +1,10 @@ repos: - repo: https://github.com/pre-commit/mirrors-prettier - rev: "v2.7.1" + rev: "v3.1.0" hooks: - id: prettier + - repo: https://github.com/editorconfig-checker/editorconfig-checker.python + rev: "2.7.3" + hooks: + - id: editorconfig-checker + alias: ec diff --git a/CHANGELOG.md b/CHANGELOG.md index 8839e0f7..72746e18 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,7 +3,7 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.5.0dev - [date] +## v2.6.0dev - [date] Initial release of nf-core/scrnaseq, created with the [nf-core](https://nf-co.re/) template. diff --git a/README.md b/README.md index 4736c821..f997b3d7 100644 --- a/README.md +++ b/README.md @@ -1,5 +1,9 @@ -# ![nf-core/scrnaseq](docs/images/nf-core-scrnaseq_logo_light.png#gh-light-mode-only) ![nf-core/scrnaseq](docs/images/nf-core-scrnaseq_logo_dark.png#gh-dark-mode-only) - +

+ + + nf-core/scrnaseq + +

[![GitHub Actions CI Status](https://github.com/nf-core/scrnaseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/scrnaseq/actions?query=workflow%3A%22nf-core+CI%22) [![GitHub Actions Linting Status](https://github.com/nf-core/scrnaseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/scrnaseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/scrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) diff --git a/assets/email_template.html b/assets/email_template.html index 2ff8db51..d5ed3696 100644 --- a/assets/email_template.html +++ b/assets/email_template.html @@ -12,7 +12,7 @@ -

nf-core/scrnaseq v${version}

+

nf-core/scrnaseq ${version}

Run Name: $runName

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checkProfile(workflow.profile, args, log) // Check that conda channels are set-up correctly if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { @@ -60,4 +62,16 @@ class WorkflowMain { } return null } + + // + // Exit pipeline if --profile contains spaces + // + private static void checkProfile(profile, args, log) { + if (profile.endsWith(',')) { + Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + if (args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." + } + } } diff --git a/lib/nfcore_external_java_deps.jar b/lib/nfcore_external_java_deps.jar deleted file mode 100644 index 805c8bb5e4fd43a12a5891eea5a68788309629b0..0000000000000000000000000000000000000000 GIT binary patch literal 0 HcmV?d00001 literal 2291171 zcma%i1CS<7mu}m(-Ea4_?dhK8v~AnAZQHhO+qP}n-E-&Lf8*Yb{bM(7MpZ;s){{|L znGpxiIY(9k6buar3JMCSNkdW|=)W{*ATS^)VMTsgF=-L{?{Od?P#{?eNT`1hApZ%I z{U0Zz{YCuW$x{5%Vj{u{igZ#U4^op8k`lCZbMO+hRMV3)4e|_&%)3YSbmCGpVp4NX zni_mKLGakt&P12{r^KCD0GPa9sIuy`fu+agt?8O-hY9i{WloGe}y@kSsDEY_Fud9 z-?2ab4J)MQXe4E3Xl-U<>PRMQZ)0m@@90KnV5#Te5Tz`pvZ0Lj4F<9%Flh(`Qb;Tn zMV!iFmH{S^fF*j#MCKRUd`7m>9||*;WE32I_6pg4-Q1C#ke$$aVmP-TtfJ+j+N9;Q z>bxN=bCVQkg$Jzty71(+~A9}ZfANuYIVnTdW!??Sj)Ti-Qpk8XgPDab8pk2QD&dwUl=iFhd1c$C2uMVagr zWc@z9s$#g__{3?VGb#)^fSNp^lZh zU>MI!D?+L)nyth{@@L0+B&_M8iR08L)G6#PZQLFmvz-9DlZkwRWigRIyI4xYr7>tRJ-N6MlBB`nJQ!!8D)mI2lB3PP;dC{<{Td>^ 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b/modules.json index 47755872..364c63d0 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "custom/dumpsoftwareversions": { "branch": "master", - "git_sha": "bba7e362e4afead70653f84d8700588ea28d0f9e", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] }, "fastqc": { "branch": "master", - "git_sha": "65ad3e0b9a4099592e1102e92e10455dc661cf53", + "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "4ab13872435962dadc239979554d13709e20bf29", + "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", "installed_by": ["modules"] } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml index f0c63f69..9b3272bc 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ b/modules/nf-core/custom/dumpsoftwareversions/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.17 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf index 7685b33c..f2187611 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ b/modules/nf-core/custom/dumpsoftwareversions/main.nf @@ -4,8 +4,8 @@ process CUSTOM_DUMPSOFTWAREVERSIONS { // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.17--pyhdfd78af_0' : - 'biocontainers/multiqc:1.17--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path versions diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test index eec1db10..b1e1630b 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test @@ -31,7 +31,12 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(process.out).match() } + { assert snapshot( + process.out.versions, + file(process.out.mqc_yml[0]).readLines()[0..10], + file(process.out.yml[0]).readLines()[0..7] + ).match() + } ) } } diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap index 4274ed57..5f59a936 100644 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap @@ -1,27 +1,33 @@ { "Should run without failures": { "content": [ - { - "0": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ], - "1": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "2": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "mqc_yml": [ - "software_versions_mqc.yml:md5,2570f4ba271ad08357b0d3d32a9cf84d" - ], - "versions": [ - "versions.yml:md5,3843ac526e762117eedf8825b40683df" - ], - "yml": [ - "software_versions.yml:md5,1c851188476409cda5752ce971b20b58" - ] - } + [ + "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" + ], + [ + "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", + " \\n\\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n \\n \\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\", + " \\ \\n\\n\\n\\n\\n\\n \\n\\", + " \\ \\n \\n\\n\\n\\n\\", + " \\n\\n \\n \\n\\" + ], + [ + "CUSTOM_DUMPSOFTWAREVERSIONS:", + " python: 3.11.7", + " yaml: 5.4.1", + "TOOL1:", + " tool1: 0.11.9", + "TOOL2:", + " tool2: '1.9'", + "Workflow:" + ] ], - "timestamp": "2023-11-03T14:43:22.157011" + "timestamp": "2024-01-09T23:01:18.710682" } -} +} \ No newline at end of file diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index b9e8f926..1f21c664 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -3,24 +3,20 @@ nextflow_process { name "Test Process FASTQC" script "../main.nf" process "FASTQC" + tag "modules" tag "modules_nfcore" tag "fastqc" - test("Single-Read") { + test("sarscov2 single-end [fastq]") { when { - params { - outdir = "$outputDir" - } process { """ - input[0] = [ + input[0] = Channel.of([ [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) """ } } @@ -28,82 +24,189 @@ nextflow_process { then { assertAll ( { assert process.success }, + // NOTE The report contains the date inside it, which means that the md5sum is stable per day, but not longer than that. So you can't md5sum it. // looks like this:
Mon 2 Oct 2023
test.gz
// https://github.com/nf-core/modules/pull/3903#issuecomment-1743620039 - { assert process.out.html.get(0).get(1) ==~ ".*/test_fastqc.html" }, - { assert path(process.out.html.get(0).get(1)).getText().contains("") }, - { assert snapshot(process.out.versions).match("versions") }, - { assert process.out.zip.get(0).get(1) ==~ ".*/test_fastqc.zip" } + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 paired-end [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 interleaved [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } ) } } -// TODO -// // -// // Test with paired-end data -// // -// workflow test_fastqc_paired_end { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with interleaved data -// // -// workflow test_fastqc_interleaved { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with bam data -// // -// workflow test_fastqc_bam { -// input = [ -// [id: 'test', single_end: false], // meta map -// file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with multiple samples -// // -// workflow test_fastqc_multiple { -// input = [ -// [id: 'test', single_end: false], // meta map -// [ -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), -// file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) -// ] -// ] - -// FASTQC ( input ) -// } - -// // -// // Test with custom prefix -// // -// workflow test_fastqc_custom_prefix { -// input = [ -// [ id:'mysample', single_end:true ], // meta map -// file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) -// ] - -// FASTQC ( input ) -// } + + test("sarscov2 paired-end [bam]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/test_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 multiple [fastq]") { + + when { + process { + """ + input[0] = Channel.of([ + [id: 'test', single_end: false], // meta map + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_1.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1][0] ==~ ".*/test_1_fastqc.html" }, + { assert process.out.html[0][1][1] ==~ ".*/test_2_fastqc.html" }, + { assert process.out.html[0][1][2] ==~ ".*/test_3_fastqc.html" }, + { assert process.out.html[0][1][3] ==~ ".*/test_4_fastqc.html" }, + { assert process.out.zip[0][1][0] ==~ ".*/test_1_fastqc.zip" }, + { assert process.out.zip[0][1][1] ==~ ".*/test_2_fastqc.zip" }, + { assert process.out.zip[0][1][2] ==~ ".*/test_3_fastqc.zip" }, + { assert process.out.zip[0][1][3] ==~ ".*/test_4_fastqc.zip" }, + { assert path(process.out.html[0][1][0]).text.contains("") }, + { assert path(process.out.html[0][1][1]).text.contains("") }, + { assert path(process.out.html[0][1][2]).text.contains("") }, + { assert path(process.out.html[0][1][3]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 custom_prefix") { + + when { + process { + """ + input[0] = Channel.of([ + [ id:'mysample', single_end:true ], // meta map + file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + + { assert process.out.html[0][1] ==~ ".*/mysample_fastqc.html" }, + { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, + { assert path(process.out.html[0][1]).text.contains("") }, + + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastq] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([ + [ id: 'test', single_end:true ], + [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] + ]) + """ + } + } + + then { + assertAll ( + { assert process.success }, + { assert snapshot(process.out.html.collect { file(it[1]).getName() } + + process.out.zip.collect { file(it[1]).getName() } + + process.out.versions ).match() } + ) + } + } + } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 636a32ce..5d624bb8 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,10 +1,20 @@ { + "sarscov2 single-end [fastq] - stub": { + "content": [ + [ + "test.html", + "test.zip", + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "timestamp": "2024-01-17T18:40:57.254299" + }, "versions": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2023-10-09T23:40:54+0000" + "timestamp": "2024-01-17T18:36:50.033627" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index bc0bdb5b..7625b752 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.18 + - bioconda::multiqc=1.19 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 00cc48d2..1b9f7c43 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.18--pyhdfd78af_0' : - 'biocontainers/multiqc:1.18--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : + 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" @@ -43,7 +43,7 @@ process MULTIQC { stub: """ - touch multiqc_data + mkdir multiqc_data touch multiqc_plots touch multiqc_report.html diff --git a/modules/nf-core/multiqc/meta.yml b/modules/nf-core/multiqc/meta.yml index f1aa660e..45a9bc35 100644 --- a/modules/nf-core/multiqc/meta.yml +++ b/modules/nf-core/multiqc/meta.yml @@ -1,4 +1,3 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json name: multiqc description: Aggregate results from bioinformatics analyses across many samples into a single report keywords: diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index c2dad217..d0438eda 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -7,12 +7,9 @@ nextflow_process { tag "modules_nfcore" tag "multiqc" - test("MULTIQC: FASTQC") { + test("sarscov2 single-end [fastqc]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -26,20 +23,17 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } ) } } - test("MULTIQC: FASTQC and a config file") { + test("sarscov2 single-end [fastqc] [config]") { when { - params { - outdir = "$outputDir" - } process { """ input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) @@ -53,9 +47,35 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert path(process.out.report.get(0)).exists() }, - { assert path(process.out.data.get(0)).exists() }, - { assert path(process.out.versions.get(0)).getText().contains("multiqc") } + { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, + { assert process.out.data[0] ==~ ".*/multiqc_data" }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + } + + test("sarscov2 single-end [fastqc] - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[1] = [] + input[2] = [] + input[3] = [] + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.report.collect { file(it).getName() } + + process.out.data.collect { file(it).getName() } + + process.out.plots.collect { file(it).getName() } + + process.out.versions ).match() } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap new file mode 100644 index 00000000..d37e7304 --- /dev/null +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -0,0 +1,21 @@ +{ + "versions": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:02:49.911994" + }, + "sarscov2 single-end [fastqc] - stub": { + "content": [ + [ + "multiqc_report.html", + "multiqc_data", + "multiqc_plots", + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "timestamp": "2024-01-09T23:03:14.524346" + } +} \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 68563110..5ece77c6 100644 --- a/nextflow.config +++ b/nextflow.config @@ -71,7 +71,7 @@ try { } // Load nf-core/scrnaseq custom profiles from different institutions. -// Warning: Uncomment only if a pipeline-specific instititutional config already exists on nf-core/configs! +// Warning: Uncomment only if a pipeline-specific institutional config already exists on nf-core/configs! // try { // includeConfig "${params.custom_config_base}/pipeline/scrnaseq.config" // } catch (Exception e) { @@ -91,6 +91,7 @@ profiles { podman.enabled = false shifter.enabled = false charliecloud.enabled = false + channels = ['conda-forge', 'bioconda', 'defaults'] apptainer.enabled = false } mamba { @@ -233,7 +234,7 @@ manifest { description = """Pipeline for processing 10x Genomics single cell rnaseq data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.5.0dev' + version = '2.6.0dev' doi = '' } diff --git a/pyproject.toml b/pyproject.toml index 0d62beb6..7d08e1c8 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -1,10 +1,13 @@ -# Config file for Python. Mostly used to configure linting of bin/check_samplesheet.py with Black. +# Config file for Python. Mostly used to configure linting of bin/*.py with Ruff. # Should be kept the same as nf-core/tools to avoid fighting with template synchronisation. -[tool.black] +[tool.ruff] line-length = 120 -target_version = ["py37", "py38", "py39", "py310"] +target-version = "py38" +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] +cache-dir = "~/.cache/ruff" -[tool.isort] -profile = "black" -known_first_party = ["nf_core"] -multi_line_output = 3 +[tool.ruff.isort] +known-first-party = ["nf_core"] + +[tool.ruff.per-file-ignores] +"__init__.py" = ["E402", "F401"] diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 83b66d9d..115b6b48 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -127,6 +127,13 @@ workflow.onComplete { } } +workflow.onError { + if (workflow.errorReport.contains("Process requirement exceeds available memory")) { + println("🛑 Default resources exceed availability 🛑 ") + println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") + } +} + /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ THE END From 4ef499e6d856b76e7a6010f3c0e10abb63edf940 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 29 Jan 2024 14:47:27 -0800 Subject: [PATCH 008/111] renaming parameters --- .../nf-core/kallistobustools/count/main.nf | 4 ++-- .../nf-core/kallistobustools/count/meta.yml | 10 ++++----- .../kallistobustools/ref/environment.yml | 2 +- modules/nf-core/kallistobustools/ref/main.nf | 8 +++---- modules/nf-core/kallistobustools/ref/meta.yml | 22 +++++++++---------- 5 files changed, 23 insertions(+), 23 deletions(-) diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index bfd9a625..df81a097 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -27,7 +27,7 @@ process KALLISTOBUSTOOLS_COUNT { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def cdna = t1c ? "-c1 $t1c" : '' - def introns = t2c ? "-c2 $t2c" : '' + def unprocessed = t2c ? "-c2 $t2c" : '' def memory = task.memory.toGiga() - 1 """ kb \\ @@ -36,7 +36,7 @@ process KALLISTOBUSTOOLS_COUNT { -i $index \\ -g $t2g \\ $cdna \\ - $introns \\ + $unprocessed \\ -x $technology \\ $args \\ -o ${prefix}.count \\ diff --git a/modules/nf-core/kallistobustools/count/meta.yml b/modules/nf-core/kallistobustools/count/meta.yml index 7491248c..c8763343 100644 --- a/modules/nf-core/kallistobustools/count/meta.yml +++ b/modules/nf-core/kallistobustools/count/meta.yml @@ -34,16 +34,16 @@ input: pattern: "*t2g.txt" - t1c: type: file - description: kb ref's c1 spliced_t2c file + description: kb ref's c1 cdna_t2c file pattern: "*.{cdna_t2c.txt}" - t2c: type: file - description: kb ref's c2 unspliced_t2c file - pattern: "*.{introns_t2c.txt}" + description: kb ref's c2 unprocessed_t2c file + pattern: "*.{unprocessed_t2c.txt}" - workflow_mode: type: string - description: String value defining workflow to use, can be one of "standard", "lamanno", "nucleus" - pattern: "{standard,lamanno,nucleus,kite}" + description: String value defining workflow to use, can be one of "standard", "nac", "lamanno" (obsolete) + pattern: "{standard,lamanno,nac}" - technology: type: string description: String value defining the sequencing technology used. diff --git a/modules/nf-core/kallistobustools/ref/environment.yml b/modules/nf-core/kallistobustools/ref/environment.yml index 6ae07a8c..62942e01 100644 --- a/modules/nf-core/kallistobustools/ref/environment.yml +++ b/modules/nf-core/kallistobustools/ref/environment.yml @@ -5,4 +5,4 @@ channels: - defaults dependencies: - bioconda::kb-python=0.28.2 - - conda-forge::requests>=2.23.0 + - conda-forge::requests>=2.23.0 \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/ref/main.nf b/modules/nf-core/kallistobustools/ref/main.nf index 759e8663..989f86f3 100644 --- a/modules/nf-core/kallistobustools/ref/main.nf +++ b/modules/nf-core/kallistobustools/ref/main.nf @@ -17,9 +17,9 @@ process KALLISTOBUSTOOLS_REF { path "kb_ref_out.idx" , emit: index path "t2g.txt" , emit: t2g path "cdna.fa" , emit: cdna - path "intron.fa" , optional:true, emit: intron + path "unprocessed.fa" , optional:true, emit: unprocessed path "cdna_t2c.txt" , optional:true, emit: cdna_t2c - path "intron_t2c.txt" , optional:true, emit: intron_t2c + path "unprocessed_t2c.txt" , optional:true, emit: unprocessed_t2c when: task.ext.when == null || task.ext.when @@ -49,9 +49,9 @@ process KALLISTOBUSTOOLS_REF { -i kb_ref_out.idx \\ -g t2g.txt \\ -f1 cdna.fa \\ - -f2 intron.fa \\ + -f2 unprocessed.fa \\ -c1 cdna_t2c.txt \\ - -c2 intron_t2c.txt \\ + -c2 unprocessed_t2c.txt \\ --workflow $workflow_mode \\ $fasta \\ $gtf diff --git a/modules/nf-core/kallistobustools/ref/meta.yml b/modules/nf-core/kallistobustools/ref/meta.yml index 00be5143..1ca31c57 100644 --- a/modules/nf-core/kallistobustools/ref/meta.yml +++ b/modules/nf-core/kallistobustools/ref/meta.yml @@ -26,8 +26,8 @@ input: pattern: "*.{gtf,gtf.gz}" - workflow_mode: type: string - description: String value defining workflow to use, can be one of "standard", "lamanno", "nucleus" - pattern: "{standard,lamanno,nucleus}" + description: String value defining workflow to use, can be one of "standard", "nac", "lamanno" (obsolete) + pattern: "{standard,lamanno,nac}" output: - versions: type: file @@ -43,21 +43,21 @@ output: pattern: "*t2g.{txt}" - cdna: type: file - description: Cdna fasta file + description: cDNA fasta file pattern: "*cdna.{fa}" - - intron: + - unprocessed: type: file - description: intron fasta file - pattern: "*intron.{fa}" + description: Unprocessed fasta file + pattern: "*unprocessed.{fa}" - cdna_t2c: type: file - description: cdna transcript to capture file + description: cDNA transcript to capture file pattern: "*cdna_t2c.{txt}" - - intron_t2c: + - unprocessed_t2c: type: file - description: intron transcript to capture file - pattern: "*intron_t2c.{txt}" + description: Unprocessed transcript to capture file + pattern: "*unprocessed_t2c.{txt}" authors: - - "@flowuenne" + - "@flowuenne" maintainers: - "@flowuenne" From fb6db73e2c2a928222e79ebad7ba61f2f0f239b1 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 29 Jan 2024 14:56:45 -0800 Subject: [PATCH 009/111] Memory arg might need to be before fastq list? --- modules/nf-core/kallistobustools/count/main.nf | 4 ++-- subworkflows/local/kallisto_bustools.nf | 2 +- 2 files changed, 3 insertions(+), 3 deletions(-) diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index df81a097..fac39a91 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -40,8 +40,8 @@ process KALLISTOBUSTOOLS_COUNT { -x $technology \\ $args \\ -o ${prefix}.count \\ - ${reads.join( " " )} \\ - -m ${memory}G + -m ${memory}G \\ + ${reads.join( " " )} cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/subworkflows/local/kallisto_bustools.nf b/subworkflows/local/kallisto_bustools.nf index 3210e47a..41387683 100644 --- a/subworkflows/local/kallisto_bustools.nf +++ b/subworkflows/local/kallisto_bustools.nf @@ -46,7 +46,7 @@ workflow KALLISTO_BUSTOOLS { kallisto_index = KALLISTOBUSTOOLS_REF.out.index.collect() ch_versions = ch_versions.mix(KALLISTOBUSTOOLS_REF.out.versions) t1c = KALLISTOBUSTOOLS_REF.out.cdna_t2c.ifEmpty{ [] } - t2c = KALLISTOBUSTOOLS_REF.out.intron_t2c.ifEmpty{ [] } + t2c = KALLISTOBUSTOOLS_REF.out.unprocessed_t2c.ifEmpty{ [] } } /* From 3ccd0833c6509aab95beeae252ae702ad1b7d955 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 29 Jan 2024 15:35:06 -0800 Subject: [PATCH 010/111] Update main.nf --- modules/nf-core/kallistobustools/count/main.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index fac39a91..9e860241 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -41,7 +41,7 @@ process KALLISTOBUSTOOLS_COUNT { $args \\ -o ${prefix}.count \\ -m ${memory}G \\ - ${reads.join( " " )} + ${reads.join( " " )} cat <<-END_VERSIONS > versions.yml "${task.process}": From 6d818948e0b32642fa33571a875f64b65c12a3f5 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 31 Jan 2024 11:42:31 +0100 Subject: [PATCH 011/111] minimum inclusion for module tested only with kallisto aligner (both with and without automated kallisto filtering with bustools --filter parameter) --- bin/emptydrops_cell_calling.R | 49 +++++++++++ bin/mtx_to_h5ad.py | 32 ++++++-- bin/mtx_to_seurat.R | 35 ++++++-- conf/modules.config | 18 +++- conf/test.config | 3 +- modules/local/concat_h5ad.nf | 4 +- modules/local/emptydrops.nf | 82 +++++++++++++++++++ modules/local/mtx_to_h5ad.nf | 57 +++++++++---- modules/local/mtx_to_seurat.nf | 60 ++++++++++---- .../nf-core/kallistobustools/count/main.nf | 8 +- nextflow.config | 16 ++-- subworkflows/local/kallisto_bustools.nf | 3 +- subworkflows/local/mtx_conversion.nf | 4 +- workflows/scrnaseq.nf | 43 +++++++--- 14 files changed, 337 insertions(+), 77 deletions(-) create mode 100755 bin/emptydrops_cell_calling.R create mode 100644 modules/local/emptydrops.nf diff --git a/bin/emptydrops_cell_calling.R b/bin/emptydrops_cell_calling.R new file mode 100755 index 00000000..989c2b34 --- /dev/null +++ b/bin/emptydrops_cell_calling.R @@ -0,0 +1,49 @@ +#!/usr/bin/env Rscript +library("DropletUtils") +library("Matrix") + +args <- commandArgs(trailingOnly=TRUE) + +fn_mtx <- args[1] +fn_barcodes <- args[2] +fn_genes <- args[3] +outdir <- args[4] +aligner <- args[5] + +# Read matrix/barcodes/genes +genes <- read.table(fn_genes,sep='\t') +barcodes <- read.table(fn_barcodes,sep='\t') +mtx <- readMM(fn_mtx) + +get_name <- function(file) { + name <- as.character(basename(file)) + name <- gsub('\\.gz', '', name) + return(name) +} + +# Check if barcodes are in columns, if not, transpose mtx +is_transposed<-FALSE +if (dim(barcodes)[1]!=dim(mtx)[2]){ + mtx<-t(mtx) + is_transposed<-TRUE + print('Matrix was tranposed.') +} + +# Call empty drops +e.out <- emptyDrops(mtx) +is.cell <- e.out$FDR <= 0.01 + +# Slice matrix and barcodes +mtx_filtered <-mtx[,which(is.cell),drop=FALSE] +barcodes_filtered<-barcodes[which(is.cell),] + +# If matrix was transposed early, need to transpose back +if (is_transposed){ + mtx_filtered<-t(mtx_filtered) + print('Transposing back matrix.') +} + +# Write output +writeMM(mtx_filtered,file.path(outdir,get_name(fn_mtx))) +write.table(barcodes_filtered,file=file.path(outdir,get_name(fn_barcodes)),col.names=FALSE,row.names=FALSE,sep='\t',quote=FALSE) +write.table(genes,file=file.path(outdir,get_name(fn_genes)),col.names=FALSE,row.names=FALSE,sep='\t',quote=FALSE) diff --git a/bin/mtx_to_h5ad.py b/bin/mtx_to_h5ad.py index 3282122d..cdcc0c6c 100755 --- a/bin/mtx_to_h5ad.py +++ b/bin/mtx_to_h5ad.py @@ -57,22 +57,36 @@ def input_to_adata( if verbose and (txp2gene or star_index): print("Reading in {}".format(input_data)) - if aligner == "cellranger": + # + # open main data + # + if aligner == "cellranger" and input_data.lower().endswith('.h5'): adata = _10x_h5_to_adata(input_data, sample) else: adata = _mtx_to_adata(input_data, barcode_file, feature_file, sample, aligner) + # + # open gene information + # if verbose and (txp2gene or star_index): print("Reading in {}".format(txp2gene)) - if txp2gene: - t2g = pd.read_table(txp2gene, header=None, names=["gene_id", "gene_symbol"], usecols=[1, 2]) - elif star_index: - t2g = pd.read_table( - f"{star_index}/geneInfo.tab", header=None, skiprows=1, names=["gene_id", "gene_symbol"], usecols=[0, 1] - ) - - if txp2gene or star_index: + if aligner == "cellranger" and not input_data.lower().endswith('.h5'): + # + # for cellranger workflow, we do not have a txp2gene file, so, when using this normal/manual function for empty drops + # we need to provide this information coming directly from the features.tsv file + # by not using the .h5 file for conversion, we loose the two col information: feature_types and genome + # + t2g = pd.read_table(feature_file, header=None, names=["gene_id", "gene_symbol", "feature_types"], usecols=[0, 1, 2]) + else: + if txp2gene: + t2g = pd.read_table(txp2gene, header=None, names=["gene_id", "gene_symbol"], usecols=[1, 2]) + elif star_index: + t2g = pd.read_table( + f"{star_index}/geneInfo.tab", header=None, skiprows=1, names=["gene_id", "gene_symbol"], usecols=[0, 1] + ) + + if txp2gene or star_index or (aligner == "cellranger" and not input_data.lower().endswith('.h5')): t2g = t2g.drop_duplicates(subset="gene_id").set_index("gene_id") adata.var["gene_symbol"] = t2g["gene_symbol"] diff --git a/bin/mtx_to_seurat.R b/bin/mtx_to_seurat.R index f2680838..99ce2f73 100755 --- a/bin/mtx_to_seurat.R +++ b/bin/mtx_to_seurat.R @@ -3,23 +3,40 @@ library(Seurat) args <- commandArgs(trailingOnly=TRUE) -mtx_file <- args[1] -barcode_file <- args[2] -feature_file <- args[3] -out.file <- args[4] -aligner <- args[5] +mtx_file <- args[1] +barcode_file <- args[2] +feature_file <- args[3] +out.file <- args[4] +aligner <- args[5] +is_emptydrops <- args[6] + +if (is_emptydrops == "--is_emptydrops") { + is_emptydrops <- TRUE +} else{ + is_emptydrops <- FALSE +} -if(aligner %in% c("kallisto", "alevin")) { +if (aligner %in% c( "kallisto", "alevin" )) { + print("1") # for kallisto and alevin, the features file contains only one column and matrix needs to be transposed expression.matrix <- ReadMtx( mtx = mtx_file, features = feature_file, cells = barcode_file, feature.column = 1, mtx.transpose = TRUE ) } else { - expression.matrix <- ReadMtx( - mtx = mtx_file, features = feature_file, cells = barcode_file - ) + if (aligner %in% c( "cellranger", "star" ) && is_emptydrops) { + print("2") + expression.matrix <- ReadMtx( + mtx = mtx_file, features = feature_file, cells = barcode_file, feature.column = 1 + ) + } else{ + print("3") + expression.matrix <- ReadMtx( + mtx = mtx_file, features = feature_file, cells = barcode_file + ) + } } + seurat.object <- CreateSeuratObject(counts = expression.matrix) dir.create(basename(dirname(out.file)), showWarnings = FALSE) diff --git a/conf/modules.config b/conf/modules.config index 5813926a..a84247e1 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -28,6 +28,7 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] } + withName: CUSTOM_DUMPSOFTWAREVERSIONS { publishDir = [ path: { "${params.outdir}/pipeline_info" }, @@ -45,6 +46,20 @@ process { ] } + if (!params.skip_emptydrops) { + withName: EMPTYDROPS_CELL_CALLING { + publishDir = [ + path: { "${params.outdir}/${params.aligner}" }, + mode: params.publish_dir_mode, + saveAs: { filename -> + if ( params.aligner == 'cellranger' ) "count/${meta.id}/${filename}" + else if ( params.aligner == 'kallisto' ) "${meta.id}.count/${filename}" + else "${meta.id}/${filename}" + } + ] + } + } + withName: 'MTX_TO_H5AD|CONCAT_H5AD|MTX_TO_SEURAT' { publishDir = [ path: { "${params.outdir}/${params.aligner}/mtx_conversions" }, @@ -204,11 +219,12 @@ if (params.aligner == 'kallisto') { ] } withName: KALLISTOBUSTOOLS_COUNT { + def kb_filter = (params.kb_filter) ? '--filter' : '' publishDir = [ path: { "${params.outdir}/${params.aligner}" }, mode: params.publish_dir_mode ] - ext.args = "--workflow ${params.kb_workflow}" + ext.args = "--workflow ${params.kb_workflow} ${kb_filter}" } } } diff --git a/conf/test.config b/conf/test.config index 45ee54c8..08ab1b69 100644 --- a/conf/test.config +++ b/conf/test.config @@ -20,7 +20,8 @@ params { max_time = '6.h' // Input data - input = 'https://github.com/nf-core/test-datasets/raw/scrnaseq/samplesheet-2-0.csv' + input = 'https://github.com/nf-core/test-datasets/raw/scrnaseq/samplesheet-2-0.csv' + skip_emptydrops = true // module does not work on small dataset // Genome references fasta = 'https://github.com/nf-core/test-datasets/raw/scrnaseq/reference/GRCm38.p6.genome.chr19.fa' diff --git a/modules/local/concat_h5ad.nf b/modules/local/concat_h5ad.nf index 96920f9e..cd08cbbe 100644 --- a/modules/local/concat_h5ad.nf +++ b/modules/local/concat_h5ad.nf @@ -7,7 +7,7 @@ process CONCAT_H5AD { 'biocontainers/scanpy:1.7.2--pyhdfd78af_0' }" input: - path h5ad + tuple val(input_type), path(h5ad) path samplesheet output: @@ -20,7 +20,7 @@ process CONCAT_H5AD { """ concat_h5ad.py \\ --input $samplesheet \\ - --out combined_matrix.h5ad \\ + --out combined_${input_type}_matrix.h5ad \\ --suffix "_matrix.h5ad" """ diff --git a/modules/local/emptydrops.nf b/modules/local/emptydrops.nf new file mode 100644 index 00000000..0bc03026 --- /dev/null +++ b/modules/local/emptydrops.nf @@ -0,0 +1,82 @@ +process EMPTYDROPS_CELL_CALLING { + tag "$meta.id" + label 'process_medium' + + conda "bioconda::bioconductor-dropletutils" + container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? + 'https://depot.galaxyproject.org/singularity/bioconductor-dropletutils:1.18.0--r42hf17093f_1' : + 'quay.io/biocontainers/bioconductor-dropletutils:1.18.0--r42hf17093f_1' }" + + input: + // inputs from cellranger nf-core module does not come in a single sample dir + // for each sample, the sub-folders and files come directly in array. + tuple val(meta), path(inputs) + + output: + tuple val(meta), path("emptydrops_filtered"), emit: filtered_matrices + + when: + task.ext.when == null || task.ext.when + + script: + if (params.aligner == "cellranger") { + + matrix = "raw_feature_bc_matrix/matrix.mtx.gz" + barcodes = "raw_feature_bc_matrix/barcodes.tsv.gz" + features = "raw_feature_bc_matrix/features.tsv.gz" + + } else if (params.aligner == "kallisto") { + + matrix = "counts_unfiltered/*.mtx" + barcodes = "counts_unfiltered/*.barcodes.txt" + features = "counts_unfiltered/*.genes.txt" + + } else if (params.aligner == "alevin") { + + matrix = "*_alevin_results/af_quant/alevin/quants_mat.mtx" + barcodes = "*_alevin_results/af_quant/alevin/quants_mat_rows.txt" + features = "*_alevin_results/af_quant/alevin/quants_mat_cols.txt" + + } else if (params.aligner == 'star') { + + matrix = "*.Solo.out/Gene*/raw/matrix.mtx.gz" + barcodes = "*.Solo.out/Gene*/raw/barcodes.tsv.gz" + features = "*.Solo.out/Gene*/raw/features.tsv.gz" + + } + + // + // run script + // + if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') + """ + mkdir emptydrops_filtered/ + # convert file types + for splice_type in spliced unspliced ; do + emptydrops_cell_calling.R \\ + *count/counts_unfiltered/\${splice_type}.mtx \\ + *count/counts_unfiltered/\${splice_type}.barcodes.txt \\ + *count/counts_unfiltered/\${splice_type}.genes.txt \\ + emptydrops_filtered \\ + ${params.aligner} \\ + 0 + done + """ + + else + """ + mkdir emptydrops_filtered/ + emptydrops_cell_calling.R \\ + $matrix \\ + $barcodes \\ + $features \\ + emptydrops_filtered \\ + ${params.aligner} \\ + 0 + """ + + stub: + """ + touch emptydrops_filtered/* + """ +} diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 84d98608..de5e01e8 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -15,33 +15,58 @@ process MTX_TO_H5AD { path star_index output: - path "${meta.id}/*h5ad", emit: h5ad - path "${meta.id}/*", emit: counts - path "versions.yml", emit: versions + tuple val(input_type), path("${meta.id}/*h5ad") , emit: h5ad + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when script: - // def file paths for aligners (except cellranger) - if (params.aligner == 'kallisto') { - mtx_matrix = "*count/counts_unfiltered/*.mtx" - barcodes_tsv = "*count/counts_unfiltered/*.barcodes.txt" - features_tsv = "*count/counts_unfiltered/*.genes.txt" + // check input type of inputs + input_type = (inputs.toUriString().contains('unfiltered') || inputs.toUriString().contains('raw')) ? 'raw' : 'filtered' + if (inputs.toUriString().contains('emptydrops')) { input_type = 'emptydrops' } + + // def file paths for aligners. Cellranger is normally converted with the .h5 files + // However, the emptydrops call, always generate .mtx files, thus, cellranger 'emptydrops' required a parsing + if (params.aligner in [ 'cellranger', 'cellrangerarc' ] && input_type == 'emptydrops') { + + aligner = 'cellranger' + txp2gene = '' + star_index = '' + mtx_matrix = "emptydrops_filtered/matrix.mtx" + barcodes_tsv = "emptydrops_filtered/barcodes.tsv" + features_tsv = "emptydrops_filtered/features.tsv" + + } else if (params.aligner == 'kallisto') { + + kb_pattern = (input_type == 'raw') ? 'un' : '' + mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "counts_${kb_pattern}filtered" + mtx_matrix = "${mtx_dir}/*.mtx" + barcodes_tsv = "${mtx_dir}/*.barcodes.txt" + features_tsv = "${mtx_dir}/*.genes.txt" + } else if (params.aligner == 'alevin') { - mtx_matrix = "*_alevin_results/af_quant/alevin/quants_mat.mtx" - barcodes_tsv = "*_alevin_results/af_quant/alevin/quants_mat_rows.txt" - features_tsv = "*_alevin_results/af_quant/alevin/quants_mat_cols.txt" + + // alevin does not have filtered/unfiltered results + mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : '*_alevin_results/af_quant/alevin' + mtx_matrix = "${mtx_dir}/quants_mat.mtx" + barcodes_tsv = "${mtx_dir}/quants_mat_rows.txt" + features_tsv = "${mtx_dir}/quants_mat_cols.txt" + } else if (params.aligner == 'star') { - mtx_matrix = "*.Solo.out/Gene*/filtered/matrix.mtx.gz" - barcodes_tsv = "*.Solo.out/Gene*/filtered/barcodes.tsv.gz" - features_tsv = "*.Solo.out/Gene*/filtered/features.tsv.gz" + + mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "*.Solo.out/Gene*/${input_type}" + suffix = (input_type == 'emptydrops') ? '' : '.gz' + mtx_matrix = "${mtx_dir}/matrix.mtx${suffix}" + barcodes_tsv = "${mtx_dir}/barcodes.tsv${suffix}" + features_tsv = "${mtx_dir}/features.tsv${suffix}" + } // // run script // - if (params.aligner in [ 'cellranger', 'cellrangerarc' ]) + if (params.aligner in [ 'cellranger', 'cellrangerarc' ] && input_type != 'emptydrops') """ # convert file types mtx_to_h5ad.py \\ @@ -79,7 +104,7 @@ process MTX_TO_H5AD { --feature $features_tsv \\ --txp2gene ${txp2gene} \\ --star_index ${star_index} \\ - --out ${meta.id}/${meta.id}_matrix.h5ad + --out ${meta.id}/${meta.id}_${input_type}_matrix.h5ad """ stub: diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index d83575a4..212b2cbf 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -19,23 +19,49 @@ process MTX_TO_SEURAT { script: def aligner = params.aligner + + // check input type of inputs + def input_type = (inputs.toUriString().contains('unfiltered') || inputs.toUriString().contains('raw')) ? 'raw' : 'filtered' + if (inputs.toUriString().contains('emptydrops')) { input_type = 'emptydrops' } + def is_emptydrops = (input_type == 'emptydrops') ? '--is_emptydrops' : '0' + + // def file paths for aligners. Cellranger is normally converted with the .h5 files + // However, the emptydrops call, always generate .mtx files, thus, cellranger 'emptydrops' required a parsing if (params.aligner in [ 'cellranger', 'cellrangerarc' ]) { - matrix = "matrix.mtx.gz" - barcodes = "barcodes.tsv.gz" - features = "features.tsv.gz" - } else if (params.aligner == "kallisto") { - matrix = "*count/counts_unfiltered/*.mtx" - barcodes = "*count/counts_unfiltered/*.barcodes.txt" - features = "*count/counts_unfiltered/*.genes.txt" + + mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "${input_type}_feature_bc_matrix" + matrix = "${mtx_dir}/matrix.mtx*" + barcodes = "${mtx_dir}/barcodes.tsv*" + features = "${mtx_dir}/features.tsv*" + + } else if (params.aligner == 'kallisto') { + + kb_pattern = (input_type == 'raw') ? 'un' : '' + mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "counts_${kb_pattern}filtered" + matrix = "${mtx_dir}/*.mtx" + barcodes = "${mtx_dir}/*.barcodes.txt" + features = "${mtx_dir}/*.genes.txt" + } else if (params.aligner == "alevin") { - matrix = "*_alevin_results/af_quant/alevin/quants_mat.mtx" - barcodes = "*_alevin_results/af_quant/alevin/quants_mat_rows.txt" - features = "*_alevin_results/af_quant/alevin/quants_mat_cols.txt" + + mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : '*_alevin_results/af_quant/alevin' + matrix = "${mtx_dir}/quants_mat.mtx" + barcodes = "${mtx_dir}/quants_mat_rows.txt" + features = "${mtx_dir}/quants_mat_cols.txt" + } else if (params.aligner == 'star') { - matrix = "*.Solo.out/Gene*/filtered/matrix.mtx.gz" - barcodes = "*.Solo.out/Gene*/filtered/barcodes.tsv.gz" - features = "*.Solo.out/Gene*/filtered/features.tsv.gz" + + mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "*.Solo.out/Gene*/${input_type}" + suffix = (input_type == 'emptydrops') ? '' : '.gz' + matrix = "${mtx_dir}/matrix.mtx${suffix}" + barcodes = "${mtx_dir}/barcodes.tsv${suffix}" + features = "${mtx_dir}/features.tsv${suffix}" + } + + // + // run script + // """ mkdir ${meta.id} """ @@ -49,7 +75,8 @@ process MTX_TO_SEURAT { *count/counts_unfiltered/\${input_type}.barcodes.txt \\ *count/counts_unfiltered/\${input_type}.genes.txt \\ ${meta.id}/${meta.id}_\${input_type}_matrix.rds \\ - ${aligner} + ${aligner} \\ + ${is_emptydrops} done """ @@ -59,8 +86,9 @@ process MTX_TO_SEURAT { $matrix \\ $barcodes \\ $features \\ - ${meta.id}/${meta.id}_matrix.rds \\ - ${aligner} + ${meta.id}/${meta.id}_${input_type}_matrix.rds \\ + ${aligner} \\ + ${is_emptydrops} """ stub: diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index 036bb35d..f29af40b 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -16,9 +16,11 @@ process KALLISTOBUSTOOLS_COUNT { val technology output: - tuple val(meta), path ("*.count"), emit: count - path "versions.yml" , emit: versions - path "*.count/*/*.mtx" , emit: matrix //Ensure that kallisto finished and produced outputs + tuple val(meta), path ("*.count") , emit: count + tuple val(meta), path ("*.count/counts_unfiltered"), emit: raw_counts // TODO: Add to nf-coew/modules before merging PR + tuple val(meta), path ("*.count/counts_filtered") , emit: filtered_counts, optional: true // TODO: Add to nf-coew/modules before merging PR + path "versions.yml" , emit: versions + path "*.count/*/*.mtx" , emit: matrix //Ensure that kallisto finished and produced outputs when: task.ext.when == null || task.ext.when diff --git a/nextflow.config b/nextflow.config index 7af20f8c..bf3133fa 100644 --- a/nextflow.config +++ b/nextflow.config @@ -26,10 +26,11 @@ params { txp2gene = null salmon_index = null - // kallist bustools parameters + // kallisto bustools parameters kallisto_gene_map = null kallisto_index = null kb_workflow = "standard" + kb_filter = false // STARsolo parameters star_index = null @@ -38,15 +39,18 @@ params { star_feature = "Gene" // Cellranger parameters - cellranger_index = null + cellranger_index = null // Cellranger ARC parameters - motifs = null - cellrangerarc_config = null + motifs = null + cellrangerarc_config = null cellrangerarc_reference = null - // UniverSC paramaters - universc_index = null + // UniverSC parameters + universc_index = null + + // Emptydrops parameters + skip_emptydrops = false // Template Boilerplate options skip_multiqc = false diff --git a/subworkflows/local/kallisto_bustools.nf b/subworkflows/local/kallisto_bustools.nf index 3210e47a..3b4b6b82 100644 --- a/subworkflows/local/kallisto_bustools.nf +++ b/subworkflows/local/kallisto_bustools.nf @@ -66,7 +66,8 @@ workflow KALLISTO_BUSTOOLS { emit: ch_versions counts = KALLISTOBUSTOOLS_COUNT.out.count + raw_counts = KALLISTOBUSTOOLS_COUNT.out.raw_counts + filtered_counts = KALLISTOBUSTOOLS_COUNT.out.filtered_counts txp2gene = txp2gene.collect() - } diff --git a/subworkflows/local/mtx_conversion.nf b/subworkflows/local/mtx_conversion.nf index 958da400..8d1caee4 100644 --- a/subworkflows/local/mtx_conversion.nf +++ b/subworkflows/local/mtx_conversion.nf @@ -35,7 +35,7 @@ workflow MTX_CONVERSION { // Concat sample-specific h5ad in one // CONCAT_H5AD ( - MTX_TO_H5AD.out.h5ad.collect(), // gather all sample-specific files + MTX_TO_H5AD.out.h5ad.groupTuple(), // gather all sample-specific files / per type samplesheet ) @@ -51,6 +51,6 @@ workflow MTX_CONVERSION { emit: ch_versions - counts = MTX_TO_H5AD.out.counts + // counts = MTX_TO_H5AD.out.counts was this ever used? } diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 25740a8e..596ce419 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -37,16 +37,17 @@ ch_multiqc_custom_methods_description = params.multiqc_methods_description ? fil // // SUBWORKFLOW: Consisting of a mix of local and nf-core/modules // -include { INPUT_CHECK } from '../subworkflows/local/input_check' -include { FASTQC_CHECK } from '../subworkflows/local/fastqc' -include { KALLISTO_BUSTOOLS } from '../subworkflows/local/kallisto_bustools' -include { SCRNASEQ_ALEVIN } from '../subworkflows/local/alevin' -include { STARSOLO } from '../subworkflows/local/starsolo' -include { CELLRANGER_ALIGN } from "../subworkflows/local/align_cellranger" -include { CELLRANGERARC_ALIGN } from "../subworkflows/local/align_cellrangerarc" -include { UNIVERSC_ALIGN } from "../subworkflows/local/align_universc" -include { MTX_CONVERSION } from "../subworkflows/local/mtx_conversion" -include { GTF_GENE_FILTER } from '../modules/local/gtf_gene_filter' +include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { FASTQC_CHECK } from '../subworkflows/local/fastqc' +include { KALLISTO_BUSTOOLS } from '../subworkflows/local/kallisto_bustools' +include { SCRNASEQ_ALEVIN } from '../subworkflows/local/alevin' +include { STARSOLO } from '../subworkflows/local/starsolo' +include { CELLRANGER_ALIGN } from "../subworkflows/local/align_cellranger" +include { CELLRANGERARC_ALIGN } from "../subworkflows/local/align_cellrangerarc" +include { UNIVERSC_ALIGN } from "../subworkflows/local/align_universc" +include { MTX_CONVERSION } from "../subworkflows/local/mtx_conversion" +include { GTF_GENE_FILTER } from '../modules/local/gtf_gene_filter' +include { EMPTYDROPS_CELL_CALLING } from '../modules/local/emptydrops' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -149,7 +150,7 @@ workflow SCRNASEQ { ch_fastq ) ch_versions = ch_versions.mix(KALLISTO_BUSTOOLS.out.ch_versions) - ch_mtx_matrices = ch_mtx_matrices.mix(KALLISTO_BUSTOOLS.out.counts) + ch_mtx_matrices = ch_mtx_matrices.mix(KALLISTO_BUSTOOLS.out.raw_counts, KALLISTO_BUSTOOLS.out.filtered_counts) ch_txp2gene = KALLISTO_BUSTOOLS.out.txp2gene } @@ -232,6 +233,26 @@ workflow SCRNASEQ { ch_mtx_matrices = ch_mtx_matrices.mix(CELLRANGERARC_ALIGN.out.cellranger_arc_out) } + // Run emptydrops calling module + if ( !params.skip_emptydrops ) { + // emptydrops should only run on the raw matrices thus, filter-out the filtered results + // of the aligners that can produce it + if ( params.aligner == "cellranger" ) { + ch_mtx_matrices_for_emptydrops = + ch_mtx_matrices.map { meta, mtx_files -> + [ meta, mtx_files.findAll { it.toString().contains("raw_feature_bc_matrix") } ] + } + .filter { meta, mtx_files -> mtx_files } + } else if (params.aligner == 'kallisto') { + ch_mtx_matrices_for_emptydrops = + ch_mtx_matrices.filter { meta, mtx_files -> mtx_files.toUriString().contains("counts_unfiltered") } + } else { + ch_mtx_matrices_for_emptydrops = ch_mtx_matrices + } + EMPTYDROPS_CELL_CALLING( ch_mtx_matrices_for_emptydrops ) + ch_mtx_matrices = ch_mtx_matrices.mix( EMPTYDROPS_CELL_CALLING.out.filtered_matrices ) + } + // Run mtx to h5ad conversion subworkflow MTX_CONVERSION ( ch_mtx_matrices, From afe4904eb6faf381ceebe5fd3c21476210fc6d1f Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Thu, 1 Feb 2024 12:19:45 +0100 Subject: [PATCH 012/111] update input_type labels --- modules/local/mtx_to_h5ad.nf | 15 ++++++++------- modules/local/mtx_to_seurat.nf | 20 ++++++++++++-------- 2 files changed, 20 insertions(+), 15 deletions(-) diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index de5e01e8..08bb18a2 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -24,11 +24,12 @@ process MTX_TO_H5AD { script: // check input type of inputs input_type = (inputs.toUriString().contains('unfiltered') || inputs.toUriString().contains('raw')) ? 'raw' : 'filtered' - if (inputs.toUriString().contains('emptydrops')) { input_type = 'emptydrops' } + if ( params.aligner == 'alevin' ) { input_type = 'raw' } // alevin has its own filtering methods and mostly output a single mtx, raw here means, the base tool output + if (inputs.toUriString().contains('emptydrops')) { input_type = 'custom_emptydrops_filter' } // def file paths for aligners. Cellranger is normally converted with the .h5 files // However, the emptydrops call, always generate .mtx files, thus, cellranger 'emptydrops' required a parsing - if (params.aligner in [ 'cellranger', 'cellrangerarc' ] && input_type == 'emptydrops') { + if (params.aligner in [ 'cellranger', 'cellrangerarc' ] && input_type == 'custom_emptydrops_filter') { aligner = 'cellranger' txp2gene = '' @@ -40,7 +41,7 @@ process MTX_TO_H5AD { } else if (params.aligner == 'kallisto') { kb_pattern = (input_type == 'raw') ? 'un' : '' - mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "counts_${kb_pattern}filtered" + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "counts_${kb_pattern}filtered" mtx_matrix = "${mtx_dir}/*.mtx" barcodes_tsv = "${mtx_dir}/*.barcodes.txt" features_tsv = "${mtx_dir}/*.genes.txt" @@ -48,15 +49,15 @@ process MTX_TO_H5AD { } else if (params.aligner == 'alevin') { // alevin does not have filtered/unfiltered results - mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : '*_alevin_results/af_quant/alevin' + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : '*_alevin_results/af_quant/alevin' mtx_matrix = "${mtx_dir}/quants_mat.mtx" barcodes_tsv = "${mtx_dir}/quants_mat_rows.txt" features_tsv = "${mtx_dir}/quants_mat_cols.txt" } else if (params.aligner == 'star') { - mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "*.Solo.out/Gene*/${input_type}" - suffix = (input_type == 'emptydrops') ? '' : '.gz' + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "*.Solo.out/Gene*/${input_type}" + suffix = (input_type == 'custom_emptydrops_filter') ? '' : '.gz' mtx_matrix = "${mtx_dir}/matrix.mtx${suffix}" barcodes_tsv = "${mtx_dir}/barcodes.tsv${suffix}" features_tsv = "${mtx_dir}/features.tsv${suffix}" @@ -66,7 +67,7 @@ process MTX_TO_H5AD { // // run script // - if (params.aligner in [ 'cellranger', 'cellrangerarc' ] && input_type != 'emptydrops') + if (params.aligner in [ 'cellranger', 'cellrangerarc' ] && input_type != 'custom_emptydrops_filter') """ # convert file types mtx_to_h5ad.py \\ diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index 212b2cbf..d95770d2 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -21,15 +21,19 @@ process MTX_TO_SEURAT { def aligner = params.aligner // check input type of inputs - def input_type = (inputs.toUriString().contains('unfiltered') || inputs.toUriString().contains('raw')) ? 'raw' : 'filtered' - if (inputs.toUriString().contains('emptydrops')) { input_type = 'emptydrops' } - def is_emptydrops = (input_type == 'emptydrops') ? '--is_emptydrops' : '0' + def is_emptydrops = '0' + input_type = (inputs.toUriString().contains('unfiltered') || inputs.toUriString().contains('raw')) ? 'raw' : 'filtered' + if ( params.aligner == 'alevin' ) { input_type = 'raw' } // alevin has its own filtering methods and mostly output a single mtx, raw here means, the base tool output + if (inputs.toUriString().contains('emptydrops')) { + input_type = 'custom_emptydrops_filter' + is_emptydrops = '--is_emptydrops' + } // def file paths for aligners. Cellranger is normally converted with the .h5 files // However, the emptydrops call, always generate .mtx files, thus, cellranger 'emptydrops' required a parsing if (params.aligner in [ 'cellranger', 'cellrangerarc' ]) { - mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "${input_type}_feature_bc_matrix" + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "${input_type}_feature_bc_matrix" matrix = "${mtx_dir}/matrix.mtx*" barcodes = "${mtx_dir}/barcodes.tsv*" features = "${mtx_dir}/features.tsv*" @@ -37,22 +41,22 @@ process MTX_TO_SEURAT { } else if (params.aligner == 'kallisto') { kb_pattern = (input_type == 'raw') ? 'un' : '' - mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "counts_${kb_pattern}filtered" + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "counts_${kb_pattern}filtered" matrix = "${mtx_dir}/*.mtx" barcodes = "${mtx_dir}/*.barcodes.txt" features = "${mtx_dir}/*.genes.txt" } else if (params.aligner == "alevin") { - mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : '*_alevin_results/af_quant/alevin' + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : '*_alevin_results/af_quant/alevin' matrix = "${mtx_dir}/quants_mat.mtx" barcodes = "${mtx_dir}/quants_mat_rows.txt" features = "${mtx_dir}/quants_mat_cols.txt" } else if (params.aligner == 'star') { - mtx_dir = (input_type == 'emptydrops') ? 'emptydrops_filtered' : "*.Solo.out/Gene*/${input_type}" - suffix = (input_type == 'emptydrops') ? '' : '.gz' + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "*.Solo.out/Gene*/${input_type}" + suffix = (input_type == 'custom_emptydrops_filter') ? '' : '.gz' matrix = "${mtx_dir}/matrix.mtx${suffix}" barcodes = "${mtx_dir}/barcodes.tsv${suffix}" features = "${mtx_dir}/features.tsv${suffix}" From 59648cc6f3ad49fbb4a0fffd22769c8e11773154 Mon Sep 17 00:00:00 2001 From: f641l Date: Fri, 2 Feb 2024 11:07:25 +0100 Subject: [PATCH 013/111] Fixing issue #297 --- assets/protocols.json | 5 +++++ workflows/scrnaseq.nf | 2 +- 2 files changed, 6 insertions(+), 1 deletion(-) diff --git a/assets/protocols.json b/assets/protocols.json index 23ff1328..d8da2f8a 100644 --- a/assets/protocols.json +++ b/assets/protocols.json @@ -30,6 +30,11 @@ "protocol": "SC3Pv3" } }, + "cellrangerarc": { + "auto": { + "protocol": "auto" + } + }, "star": { "10XV1": { "protocol": "CB_UMI_Simple", diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 25740a8e..1e4932ee 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -71,7 +71,7 @@ include { MULTIQC } from '../modules/nf-core/multiqc/main' ch_output_docs = file("$projectDir/docs/output.md", checkIfExists: true) ch_output_docs_images = file("$projectDir/docs/images/", checkIfExists: true) protocol_config = WorkflowScrnaseq.getProtocol(workflow, log, params.aligner, params.protocol) -if (protocol_config['protocol'] == 'auto' && params.aligner != "cellranger") { +if (protocol_config['protocol'] == 'auto' && ( params.aligner != "cellranger" && params.aligner != "cellrangerarc") ) { error "Only cellranger supports `protocol = 'auto'`. Please specify the protocol manually!" } From d03d81f5643219f70af23678e59fe2a6751ce8fc Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Fri, 2 Feb 2024 11:23:08 +0100 Subject: [PATCH 014/111] fixed workflow conversions to work with star align results --- modules/local/emptydrops.nf | 6 +++--- modules/local/mtx_to_h5ad.nf | 2 +- modules/local/mtx_to_seurat.nf | 2 +- modules/local/star_align.nf | 14 ++++++++------ subworkflows/local/starsolo.nf | 2 ++ workflows/scrnaseq.nf | 22 +++++++++++----------- 6 files changed, 26 insertions(+), 22 deletions(-) diff --git a/modules/local/emptydrops.nf b/modules/local/emptydrops.nf index 0bc03026..215b6da3 100644 --- a/modules/local/emptydrops.nf +++ b/modules/local/emptydrops.nf @@ -39,9 +39,9 @@ process EMPTYDROPS_CELL_CALLING { } else if (params.aligner == 'star') { - matrix = "*.Solo.out/Gene*/raw/matrix.mtx.gz" - barcodes = "*.Solo.out/Gene*/raw/barcodes.tsv.gz" - features = "*.Solo.out/Gene*/raw/features.tsv.gz" + matrix = "raw/matrix.mtx.gz" + barcodes = "raw/barcodes.tsv.gz" + features = "raw/features.tsv.gz" } diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 08bb18a2..7afc12fa 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -56,7 +56,7 @@ process MTX_TO_H5AD { } else if (params.aligner == 'star') { - mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "*.Solo.out/Gene*/${input_type}" + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "${input_type}" suffix = (input_type == 'custom_emptydrops_filter') ? '' : '.gz' mtx_matrix = "${mtx_dir}/matrix.mtx${suffix}" barcodes_tsv = "${mtx_dir}/barcodes.tsv${suffix}" diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index d95770d2..8e991c24 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -55,7 +55,7 @@ process MTX_TO_SEURAT { } else if (params.aligner == 'star') { - mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "*.Solo.out/Gene*/${input_type}" + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "${input_type}" suffix = (input_type == 'custom_emptydrops_filter') ? '' : '.gz' matrix = "${mtx_dir}/matrix.mtx${suffix}" barcodes = "${mtx_dir}/barcodes.tsv${suffix}" diff --git a/modules/local/star_align.nf b/modules/local/star_align.nf index a7dfab3d..4b3df1e1 100644 --- a/modules/local/star_align.nf +++ b/modules/local/star_align.nf @@ -21,12 +21,14 @@ process STAR_ALIGN { val other_10x_parameters output: - tuple val(meta), path('*d.out.bam') , emit: bam - tuple val(meta), path('*.Solo.out') , emit: counts - tuple val(meta), path('*Log.final.out') , emit: log_final - tuple val(meta), path('*Log.out') , emit: log_out - tuple val(meta), path('*Log.progress.out'), emit: log_progress - path "versions.yml" , emit: versions + tuple val(meta), path('*d.out.bam') , emit: bam + tuple val(meta), path('*.Solo.out') , emit: counts + tuple val(meta), path ("*.Solo.out/Gene*/raw") , emit: raw_counts + tuple val(meta), path ("*.Solo.out/Gene*/filtered"), emit: filtered_counts + tuple val(meta), path('*Log.final.out') , emit: log_final + tuple val(meta), path('*Log.out') , emit: log_out + tuple val(meta), path('*Log.progress.out') , emit: log_progress + path "versions.yml" , emit: versions tuple val(meta), path('*sortedByCoord.out.bam') , optional:true, emit: bam_sorted tuple val(meta), path('*toTranscriptome.out.bam'), optional:true, emit: bam_transcript diff --git a/subworkflows/local/starsolo.nf b/subworkflows/local/starsolo.nf index 47b2e757..9056d899 100644 --- a/subworkflows/local/starsolo.nf +++ b/subworkflows/local/starsolo.nf @@ -60,5 +60,7 @@ workflow STARSOLO { star_index = star_index.map{ meta, index -> index} star_result = STAR_ALIGN.out.tab star_counts = STAR_ALIGN.out.counts + raw_counts = STAR_ALIGN.out.raw_counts + filtered_counts = STAR_ALIGN.out.filtered_counts for_multiqc = STAR_ALIGN.out.log_final } diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 596ce419..71abb7a2 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -184,7 +184,7 @@ workflow SCRNASEQ { protocol_config.get('extra_args', ""), ) ch_versions = ch_versions.mix(STARSOLO.out.ch_versions) - ch_mtx_matrices = ch_mtx_matrices.mix(STARSOLO.out.star_counts) + ch_mtx_matrices = ch_mtx_matrices.mix(STARSOLO.out.raw_counts, STARSOLO.out.filtered_counts) ch_star_index = STARSOLO.out.star_index ch_multiqc_star = STARSOLO.out.for_multiqc } @@ -235,17 +235,17 @@ workflow SCRNASEQ { // Run emptydrops calling module if ( !params.skip_emptydrops ) { - // emptydrops should only run on the raw matrices thus, filter-out the filtered results - // of the aligners that can produce it - if ( params.aligner == "cellranger" ) { - ch_mtx_matrices_for_emptydrops = - ch_mtx_matrices.map { meta, mtx_files -> - [ meta, mtx_files.findAll { it.toString().contains("raw_feature_bc_matrix") } ] - } - .filter { meta, mtx_files -> mtx_files } - } else if (params.aligner == 'kallisto') { + + // + // emptydrops should only run on the raw matrices thus, filter-out the filtered result of the aligners that can produce it + // + if ( params.aligner in [ 'cellranger', 'cellrangerarc', 'kallisto', 'star' ] ) { ch_mtx_matrices_for_emptydrops = - ch_mtx_matrices.filter { meta, mtx_files -> mtx_files.toUriString().contains("counts_unfiltered") } + ch_mtx_matrices.filter { meta, mtx_files -> + mtx_files.toString().contains("raw_feature_bc_matrix") || // cellranger + mtx_files.toUriString().contains("counts_unfiltered") || // kallisto + mtx_files.toUriString().contains("raw") // star + } } else { ch_mtx_matrices_for_emptydrops = ch_mtx_matrices } From 3324a7873b3f85dc4dbe577eebe0f0d546363472 Mon Sep 17 00:00:00 2001 From: f641l Date: Fri, 2 Feb 2024 11:49:08 +0100 Subject: [PATCH 015/111] fix issue 299 --- modules/local/mtx_to_seurat.nf | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index d83575a4..73e260d2 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -20,9 +20,9 @@ process MTX_TO_SEURAT { script: def aligner = params.aligner if (params.aligner in [ 'cellranger', 'cellrangerarc' ]) { - matrix = "matrix.mtx.gz" - barcodes = "barcodes.tsv.gz" - features = "features.tsv.gz" + matrix = "filtered_feature_bc_matrix/matrix.mtx.gz" + barcodes = "filtered_feature_bc_matrix/barcodes.tsv.gz" + features = "filtered_feature_bc_matrix/features.tsv.gz" } else if (params.aligner == "kallisto") { matrix = "*count/counts_unfiltered/*.mtx" barcodes = "*count/counts_unfiltered/*.barcodes.txt" From f9387446e889eb32e755765e9a81b0f1d8d749de Mon Sep 17 00:00:00 2001 From: f641l Date: Fri, 2 Feb 2024 13:47:51 +0100 Subject: [PATCH 016/111] Fix for Issue 229 --- modules/local/mtx_to_seurat.nf | 6 +++--- modules/nf-core/cellrangerarc/count/main.nf | 2 +- 2 files changed, 4 insertions(+), 4 deletions(-) diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index 73e260d2..d83575a4 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -20,9 +20,9 @@ process MTX_TO_SEURAT { script: def aligner = params.aligner if (params.aligner in [ 'cellranger', 'cellrangerarc' ]) { - matrix = "filtered_feature_bc_matrix/matrix.mtx.gz" - barcodes = "filtered_feature_bc_matrix/barcodes.tsv.gz" - features = "filtered_feature_bc_matrix/features.tsv.gz" + matrix = "matrix.mtx.gz" + barcodes = "barcodes.tsv.gz" + features = "features.tsv.gz" } else if (params.aligner == "kallisto") { matrix = "*count/counts_unfiltered/*.mtx" barcodes = "*count/counts_unfiltered/*.barcodes.txt" diff --git a/modules/nf-core/cellrangerarc/count/main.nf b/modules/nf-core/cellrangerarc/count/main.nf index 2bf0193a..01eac247 100644 --- a/modules/nf-core/cellrangerarc/count/main.nf +++ b/modules/nf-core/cellrangerarc/count/main.nf @@ -14,7 +14,7 @@ process CELLRANGERARC_COUNT { path reference output: - tuple val(meta), path("${meta.id}/outs/*"), emit: outs + tuple val(meta), path("${meta.id}/outs/**"), emit: outs path("${meta.id}_lib.csv") , emit: lib path "versions.yml" , emit: versions From 796afba1e5df0ca273fcadb1bac2a04cf5a48a51 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Mon, 5 Feb 2024 10:02:27 +0100 Subject: [PATCH 017/111] include modifications for working with cellranger --- bin/mtx_to_h5ad.py | 10 +++++++--- modules/local/emptydrops.nf | 6 +++--- modules/local/mtx_to_h5ad.nf | 11 +++++++---- modules/local/mtx_to_seurat.nf | 16 ++++++++++------ modules/nf-core/cellranger/count/main.nf | 6 ++++-- subworkflows/local/align_cellranger.nf | 5 +++-- subworkflows/local/mtx_conversion.nf | 8 -------- workflows/scrnaseq.nf | 8 ++++---- 8 files changed, 38 insertions(+), 32 deletions(-) diff --git a/bin/mtx_to_h5ad.py b/bin/mtx_to_h5ad.py index cdcc0c6c..2f5dc9ba 100755 --- a/bin/mtx_to_h5ad.py +++ b/bin/mtx_to_h5ad.py @@ -32,9 +32,13 @@ def _mtx_to_adata( aligner: str, ): adata = sc.read_mtx(mtx_file) - if ( - aligner == "star" - ): # for some reason star matrix comes transposed and doesn't fit when values are appended directly + # for some reason star matrix comes transposed and doesn't fit when values are appended directly + # also true for cellranger files ( this is only used when running with the custom emptydrops_filtered files ) + # otherwise, it uses the cellranger .h5 files + if aligner in [ + "cellranger", + "star", + ]: adata = adata.transpose() adata.obs_names = pd.read_csv(barcode_file, header=None, sep="\t")[0].values diff --git a/modules/local/emptydrops.nf b/modules/local/emptydrops.nf index 215b6da3..ca42e171 100644 --- a/modules/local/emptydrops.nf +++ b/modules/local/emptydrops.nf @@ -21,9 +21,9 @@ process EMPTYDROPS_CELL_CALLING { script: if (params.aligner == "cellranger") { - matrix = "raw_feature_bc_matrix/matrix.mtx.gz" - barcodes = "raw_feature_bc_matrix/barcodes.tsv.gz" - features = "raw_feature_bc_matrix/features.tsv.gz" + matrix = "matrix.mtx.gz" + barcodes = "barcodes.tsv.gz" + features = "features.tsv.gz" } else if (params.aligner == "kallisto") { diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 7afc12fa..10b617df 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -22,10 +22,13 @@ process MTX_TO_H5AD { task.ext.when == null || task.ext.when script: + // Get a file to check input type. Some aligners bring arrays instead of a single file. + def input_to_check = (inputs instanceof String) ? inputs : inputs[0] + // check input type of inputs - input_type = (inputs.toUriString().contains('unfiltered') || inputs.toUriString().contains('raw')) ? 'raw' : 'filtered' + input_type = (input_to_check.toUriString().contains('unfiltered') || input_to_check.toUriString().contains('raw')) ? 'raw' : 'filtered' if ( params.aligner == 'alevin' ) { input_type = 'raw' } // alevin has its own filtering methods and mostly output a single mtx, raw here means, the base tool output - if (inputs.toUriString().contains('emptydrops')) { input_type = 'custom_emptydrops_filter' } + if (input_to_check.toUriString().contains('emptydrops')) { input_type = 'custom_emptydrops_filter' } // def file paths for aligners. Cellranger is normally converted with the .h5 files // However, the emptydrops call, always generate .mtx files, thus, cellranger 'emptydrops' required a parsing @@ -72,9 +75,9 @@ process MTX_TO_H5AD { # convert file types mtx_to_h5ad.py \\ --aligner cellranger \\ - --input filtered_feature_bc_matrix.h5 \\ + --input ${input_type}_feature_bc_matrix.h5 \\ --sample ${meta.id} \\ - --out ${meta.id}/${meta.id}_matrix.h5ad + --out ${meta.id}/${meta.id}_${input_type}_matrix.h5ad """ else if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index 8e991c24..626e0b97 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -20,11 +20,15 @@ process MTX_TO_SEURAT { script: def aligner = params.aligner + + // Get a file to check input type. Some aligners bring arrays instead of a single file. + def input_to_check = (inputs instanceof String) ? inputs : inputs[0] + // check input type of inputs def is_emptydrops = '0' - input_type = (inputs.toUriString().contains('unfiltered') || inputs.toUriString().contains('raw')) ? 'raw' : 'filtered' + input_type = (input_to_check.toUriString().contains('unfiltered') || input_to_check.toUriString().contains('raw')) ? 'raw' : 'filtered' if ( params.aligner == 'alevin' ) { input_type = 'raw' } // alevin has its own filtering methods and mostly output a single mtx, raw here means, the base tool output - if (inputs.toUriString().contains('emptydrops')) { + if (input_to_check.toUriString().contains('emptydrops')) { input_type = 'custom_emptydrops_filter' is_emptydrops = '--is_emptydrops' } @@ -33,10 +37,10 @@ process MTX_TO_SEURAT { // However, the emptydrops call, always generate .mtx files, thus, cellranger 'emptydrops' required a parsing if (params.aligner in [ 'cellranger', 'cellrangerarc' ]) { - mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "${input_type}_feature_bc_matrix" - matrix = "${mtx_dir}/matrix.mtx*" - barcodes = "${mtx_dir}/barcodes.tsv*" - features = "${mtx_dir}/features.tsv*" + mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered/' : '' + matrix = "${mtx_dir}matrix.mtx*" + barcodes = "${mtx_dir}barcodes.tsv*" + features = "${mtx_dir}features.tsv*" } else if (params.aligner == 'kallisto') { diff --git a/modules/nf-core/cellranger/count/main.nf b/modules/nf-core/cellranger/count/main.nf index d7a191fc..3ccf34f7 100644 --- a/modules/nf-core/cellranger/count/main.nf +++ b/modules/nf-core/cellranger/count/main.nf @@ -9,8 +9,10 @@ process CELLRANGER_COUNT { path reference output: - tuple val(meta), path("**/outs/**"), emit: outs - path "versions.yml" , emit: versions + tuple val(meta), path("**/outs/**") , emit: outs + tuple val(meta), path("**/outs/filtered_feature_bc_matrix**"), emit: filtered // TODO: Add to nf-coew/modules before merging PR + tuple val(meta), path("**/outs/raw_feature_bc_matrix**") , emit: raw // TODO: Add to nf-coew/modules before merging PR + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/subworkflows/local/align_cellranger.nf b/subworkflows/local/align_cellranger.nf index bfdd533e..39e54675 100644 --- a/subworkflows/local/align_cellranger.nf +++ b/subworkflows/local/align_cellranger.nf @@ -42,6 +42,7 @@ workflow CELLRANGER_ALIGN { emit: ch_versions - cellranger_out = CELLRANGER_COUNT.out.outs - star_index = cellranger_index + cellranger_out = CELLRANGER_COUNT.out.outs + cellranger_matrices = CELLRANGER_COUNT.out.filtered.mix( CELLRANGER_COUNT.out.raw ) + star_index = cellranger_index } diff --git a/subworkflows/local/mtx_conversion.nf b/subworkflows/local/mtx_conversion.nf index 8d1caee4..b1aff9e9 100644 --- a/subworkflows/local/mtx_conversion.nf +++ b/subworkflows/local/mtx_conversion.nf @@ -14,14 +14,6 @@ workflow MTX_CONVERSION { main: ch_versions = Channel.empty() - // Cellranger module output contains too many files which cause path collisions, we filter to the ones we need. - if (params.aligner in [ 'cellranger', 'cellrangerarc' ]) { - mtx_matrices = mtx_matrices.map { meta, mtx_files -> - [ meta, mtx_files.findAll { it.toString().contains("filtered_feature_bc_matrix") } ] - } - .filter { meta, mtx_files -> mtx_files } // Remove any that are missing the relevant files - } - // // Convert matrix to h5ad // diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 71abb7a2..b030a323 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -199,7 +199,7 @@ workflow SCRNASEQ { protocol_config['protocol'] ) ch_versions = ch_versions.mix(CELLRANGER_ALIGN.out.ch_versions) - ch_mtx_matrices = ch_mtx_matrices.mix(CELLRANGER_ALIGN.out.cellranger_out) + ch_mtx_matrices = ch_mtx_matrices.mix(CELLRANGER_ALIGN.out.cellranger_matrices) ch_star_index = CELLRANGER_ALIGN.out.star_index ch_multiqc_cellranger = CELLRANGER_ALIGN.out.cellranger_out.map{ meta, outs -> outs.findAll{ it -> it.name == "web_summary.html"} @@ -242,9 +242,9 @@ workflow SCRNASEQ { if ( params.aligner in [ 'cellranger', 'cellrangerarc', 'kallisto', 'star' ] ) { ch_mtx_matrices_for_emptydrops = ch_mtx_matrices.filter { meta, mtx_files -> - mtx_files.toString().contains("raw_feature_bc_matrix") || // cellranger - mtx_files.toUriString().contains("counts_unfiltered") || // kallisto - mtx_files.toUriString().contains("raw") // star + mtx_files.toString().contains("raw_feature_bc_matrix") || // cellranger + mtx_files.toString().contains("counts_unfiltered") || // kallisto + mtx_files.toString().contains("raw") // star } } else { ch_mtx_matrices_for_emptydrops = ch_mtx_matrices From c9c38ea26f9d2e6bb932b013818ad3adeeb3456d Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 7 Feb 2024 10:49:55 +0100 Subject: [PATCH 018/111] fix the path of matrices when running non-standard kallisto workflow (tested with lamanno) --- modules/local/emptydrops.nf | 6 +++--- modules/local/mtx_to_h5ad.nf | 8 ++++---- modules/local/mtx_to_seurat.nf | 8 ++++---- 3 files changed, 11 insertions(+), 11 deletions(-) diff --git a/modules/local/emptydrops.nf b/modules/local/emptydrops.nf index ca42e171..fb0d9791 100644 --- a/modules/local/emptydrops.nf +++ b/modules/local/emptydrops.nf @@ -54,9 +54,9 @@ process EMPTYDROPS_CELL_CALLING { # convert file types for splice_type in spliced unspliced ; do emptydrops_cell_calling.R \\ - *count/counts_unfiltered/\${splice_type}.mtx \\ - *count/counts_unfiltered/\${splice_type}.barcodes.txt \\ - *count/counts_unfiltered/\${splice_type}.genes.txt \\ + counts_unfiltered/\${splice_type}.mtx \\ + counts_unfiltered/\${splice_type}.barcodes.txt \\ + counts_unfiltered/\${splice_type}.genes.txt \\ emptydrops_filtered \\ ${params.aligner} \\ 0 diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 10b617df..4cb84c88 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -80,16 +80,16 @@ process MTX_TO_H5AD { --out ${meta.id}/${meta.id}_${input_type}_matrix.h5ad """ - else if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') + else if (params.aligner == 'kallisto' && params.kb_workflow != 'standard' && input_type != 'custom_emptydrops_filter') """ # convert file types for input_type in spliced unspliced ; do mtx_to_h5ad.py \\ --aligner ${params.aligner} \\ --sample ${meta.id} \\ - --input *count/counts_unfiltered/\${input_type}.mtx \\ - --barcode *count/counts_unfiltered/\${input_type}.barcodes.txt \\ - --feature *count/counts_unfiltered/\${input_type}.genes.txt \\ + --input ${mtx_dir}/\${input_type}.mtx \\ + --barcode ${mtx_dir}/\${input_type}.barcodes.txt \\ + --feature ${mtx_dir}/\${input_type}.genes.txt \\ --txp2gene ${txp2gene} \\ --star_index ${star_index} \\ --out ${meta.id}/${meta.id}_\${input_type}_matrix.h5ad ; diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index 626e0b97..b5042426 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -74,14 +74,14 @@ process MTX_TO_SEURAT { mkdir ${meta.id} """ - if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') + if (params.aligner == 'kallisto' && params.kb_workflow != 'standard' && input_type != 'custom_emptydrops_filter') """ # convert file types for input_type in spliced unspliced ; do mtx_to_seurat.R \\ - *count/counts_unfiltered/\${input_type}.mtx \\ - *count/counts_unfiltered/\${input_type}.barcodes.txt \\ - *count/counts_unfiltered/\${input_type}.genes.txt \\ + ${mtx_dir}/\${input_type}.mtx \\ + ${mtx_dir}/\${input_type}.barcodes.txt \\ + ${mtx_dir}/\${input_type}.genes.txt \\ ${meta.id}/${meta.id}_\${input_type}_matrix.rds \\ ${aligner} \\ ${is_emptydrops} From a9ee3f1f984303b5c22c9a269481d7ce35bd123e Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 7 Feb 2024 11:20:52 +0100 Subject: [PATCH 019/111] fix spliced/unspliced empty_drops conversion --- modules/local/mtx_to_h5ad.nf | 2 +- modules/local/mtx_to_seurat.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 4cb84c88..1b32af58 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -80,7 +80,7 @@ process MTX_TO_H5AD { --out ${meta.id}/${meta.id}_${input_type}_matrix.h5ad """ - else if (params.aligner == 'kallisto' && params.kb_workflow != 'standard' && input_type != 'custom_emptydrops_filter') + else if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') """ # convert file types for input_type in spliced unspliced ; do diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index b5042426..caadcc36 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -74,7 +74,7 @@ process MTX_TO_SEURAT { mkdir ${meta.id} """ - if (params.aligner == 'kallisto' && params.kb_workflow != 'standard' && input_type != 'custom_emptydrops_filter') + if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') """ # convert file types for input_type in spliced unspliced ; do From 0cb2a2f3de5a46974945c52c828965d115549a59 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 7 Feb 2024 11:21:13 +0100 Subject: [PATCH 020/111] solve number of list levels when having spliced / unspliced --- subworkflows/local/mtx_conversion.nf | 9 ++++++++- 1 file changed, 8 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/mtx_conversion.nf b/subworkflows/local/mtx_conversion.nf index b1aff9e9..55541f18 100644 --- a/subworkflows/local/mtx_conversion.nf +++ b/subworkflows/local/mtx_conversion.nf @@ -26,8 +26,15 @@ workflow MTX_CONVERSION { // // Concat sample-specific h5ad in one // + ch_concat_h5ad_input = MTX_TO_H5AD.out.h5ad.groupTuple() // gather all sample-specific files / per type + if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') { + // when having spliced / unspliced matrices, the collected tuple has two levels ( [[mtx_1, mtx_2]] ) + // which nextflow break because it is not a valid 'path' thus, we have to remove one level + // making it as [ mtx_1, mtx_2 ] + ch_concat_h5ad_input = ch_concat_h5ad_input.map{ type, matrices -> [ type, matrices.flatten().toList() ] } + } CONCAT_H5AD ( - MTX_TO_H5AD.out.h5ad.groupTuple(), // gather all sample-specific files / per type + ch_concat_h5ad_input, samplesheet ) From 45e0b1963f36164f6dbf6db672825b8b7b52724a Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Fri, 9 Feb 2024 16:55:00 -0800 Subject: [PATCH 021/111] Removed t2g versions call MIME-Version: 1.0 Content-Type: text/plain; charset=UTF-8 Content-Transfer-Encoding: 8bit That is not really what this argument does – it just concatenates the gene_version and transcript_version fields to IDs. --- subworkflows/local/kallisto_bustools.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/kallisto_bustools.nf b/subworkflows/local/kallisto_bustools.nf index 41387683..8de01794 100644 --- a/subworkflows/local/kallisto_bustools.nf +++ b/subworkflows/local/kallisto_bustools.nf @@ -34,7 +34,7 @@ workflow KALLISTO_BUSTOOLS { if (!txp2gene && kallisto_index) { GENE_MAP( gtf ) txp2gene = GENE_MAP.out.gene_map - ch_versions = ch_versions.mix(GENE_MAP.out.versions) + /* -- ch_versions = ch_versions.mix(GENE_MAP.out.versions) -- */ } /* From 50d3948ba65aa88644fa18bc8ee6240fe6760ce4 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 12 Feb 2024 11:18:22 -0800 Subject: [PATCH 022/111] removing t2g.py The txp2gene file should really be generated by kallisto|bustools. The t2g.py script generates a 3-column rather than 7-column t2g, which seems to be incompatible with kb count. --- modules/local/gene_map.nf | 34 ------------------------- subworkflows/local/kallisto_bustools.nf | 20 +++------------ 2 files changed, 3 insertions(+), 51 deletions(-) delete mode 100644 modules/local/gene_map.nf diff --git a/modules/local/gene_map.nf b/modules/local/gene_map.nf deleted file mode 100644 index 9fd29e0a..00000000 --- a/modules/local/gene_map.nf +++ /dev/null @@ -1,34 +0,0 @@ -/* - * Reformat design file and check validity - */ -process GENE_MAP { - tag "$gtf" - label 'process_low' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path gtf - - output: - path "transcripts_to_genes.txt" , emit: gene_map - - when: - task.ext.when == null || task.ext.when - - script: - if("${gtf}".endsWith('.gz')){ - name = "${gtf.baseName}" - unzip = "gunzip -f ${gtf}" - } else { - unzip = "" - name = "${gtf}" - } - """ - $unzip - cat $name | t2g.py --use_version > transcripts_to_genes.txt - """ -} diff --git a/subworkflows/local/kallisto_bustools.nf b/subworkflows/local/kallisto_bustools.nf index 8de01794..7edd98d5 100644 --- a/subworkflows/local/kallisto_bustools.nf +++ b/subworkflows/local/kallisto_bustools.nf @@ -1,5 +1,4 @@ /* -- IMPORT LOCAL MODULES/SUBWORKFLOWS -- */ -include { GENE_MAP } from '../../modules/local/gene_map' include {KALLISTOBUSTOOLS_COUNT } from '../../modules/nf-core/kallistobustools/count/main' /* -- IMPORT NF-CORE MODULES/SUBWORKFLOWS -- */ @@ -21,26 +20,13 @@ workflow KALLISTO_BUSTOOLS { main: ch_versions = Channel.empty() - assert kallisto_index || (genome_fasta && gtf): + assert (txp2gene && kallisto_index) || (genome_fasta && gtf): "Must provide a genome fasta file ('--fasta') and a gtf file ('--gtf') if no index is given!" - assert txp2gene || gtf: - "Must provide either a GTF file ('--gtf') or kallisto gene map ('--kallisto_gene_map') to align with kallisto bustools!" - - /* - * Generate Kallisto Gene Map if not supplied and index is given - * If no index is given, the gene map will be generated in the 'kb ref' step - */ - if (!txp2gene && kallisto_index) { - GENE_MAP( gtf ) - txp2gene = GENE_MAP.out.gene_map - /* -- ch_versions = ch_versions.mix(GENE_MAP.out.versions) -- */ - } - /* - * Generate kallisto index + * Generate kallisto index and t2g if not already present */ - if (!kallisto_index) { + if (!(txp2gene && kallisto_index)) { KALLISTOBUSTOOLS_REF( genome_fasta, gtf, kb_workflow ) txp2gene = KALLISTOBUSTOOLS_REF.out.t2g.collect() kallisto_index = KALLISTOBUSTOOLS_REF.out.index.collect() From b0d7c5d0dcd523d0c0c143d6a00a7f4c16bcbdd3 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 12 Feb 2024 11:20:40 -0800 Subject: [PATCH 023/111] Remove txp2gene.collect() call This .collect() is incompatible with passing in an existing t2g, and superfluous given the .collect() after the kb ref call. --- subworkflows/local/kallisto_bustools.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/kallisto_bustools.nf b/subworkflows/local/kallisto_bustools.nf index 7edd98d5..5b3283a4 100644 --- a/subworkflows/local/kallisto_bustools.nf +++ b/subworkflows/local/kallisto_bustools.nf @@ -52,7 +52,7 @@ workflow KALLISTO_BUSTOOLS { emit: ch_versions counts = KALLISTOBUSTOOLS_COUNT.out.count - txp2gene = txp2gene.collect() + txp2gene } From 8630ef926817af9fc071143d2e476888305e9f04 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 12 Feb 2024 13:21:15 -0800 Subject: [PATCH 024/111] add t1c/t2c parameters to schema t1c and t2c are required to run nac and lamanno count workflows. Since they are already defined when running kb ref, they cannot be easily defined through ext.args when using an existing reference --- nextflow.config | 4 +++- nextflow_schema.json | 10 ++++++++++ subworkflows/local/kallisto_bustools.nf | 2 ++ 3 files changed, 15 insertions(+), 1 deletion(-) diff --git a/nextflow.config b/nextflow.config index 9e83c42a..9c3ac851 100644 --- a/nextflow.config +++ b/nextflow.config @@ -26,10 +26,12 @@ params { txp2gene = null salmon_index = null - // kallist bustools parameters + // kallisto bustools parameters kallisto_gene_map = null kallisto_index = null kb_workflow = "standard" + kb_t1c = null + kb_t2c = null // STARsolo parameters star_index = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 34af4c64..e9ca04d9 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -213,6 +213,16 @@ "description": "Specify a path to the precomputed Kallisto index.", "fa_icon": "fas fa-fish" }, + "kb_t1c": { + "type": "string", + "description": "Specify a path to the cDNA transcripts-to-capture.", + "fa_icon": "fas fa-fish" + }, + "kb_t2c": { + "type": "string", + "description": "Specify a path to the intron transcripts-to-capture.", + "fa_icon": "fas fa-fish" + }, "kb_workflow": { "type": "string", "default": "standard", diff --git a/subworkflows/local/kallisto_bustools.nf b/subworkflows/local/kallisto_bustools.nf index 5b3283a4..7fdaf8ef 100644 --- a/subworkflows/local/kallisto_bustools.nf +++ b/subworkflows/local/kallisto_bustools.nf @@ -13,6 +13,8 @@ workflow KALLISTO_BUSTOOLS { gtf kallisto_index txp2gene + t1c + t2c protocol kb_workflow ch_fastq From e503e218326fa852fb95ac2d77d88e20fec1d1d9 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 12 Feb 2024 13:29:55 -0800 Subject: [PATCH 025/111] Fixed kallisto_gene_map documentation scrnaseq does not actually use kallisto_gene_map anywhere. Changed kallisto icon from a salmon to a rainbow (there is no bear icon, but bustools uses the Pink Floyd-esque prism image, so it seems most appropriate) --- nextflow_schema.json | 20 ++++++++++---------- 1 file changed, 10 insertions(+), 10 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index e9ca04d9..b94b6460 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -155,7 +155,7 @@ "txp2gene": { "type": "string", "description": "Path to transcript to gene mapping file. This allows the specification of a transcript to gene mapping file for Salmon Alevin and AlevinQC.", - "help_text": "> This is not the same as the `kallisto_gene_map` parameter down below and is only used by the Salmon Alevin workflow.", + "help_text": "> This is the same as the `kallisto_gene_map` parameter below.", "fa_icon": "fas fa-map-marked-alt" }, "simpleaf_rlen": { @@ -203,31 +203,31 @@ "default": "", "fa_icon": "fas fa-fish", "properties": { - "kallisto_gene_map": { - "type": "string", - "description": "Specify a Kallisto gene mapping file here. If you don't, this will be automatically created in the Kallisto workflow when specifying a valid `--gtf` file.", - "fa_icon": "fas fa-fish" - }, "kallisto_index": { "type": "string", "description": "Specify a path to the precomputed Kallisto index.", - "fa_icon": "fas fa-fish" + "fa_icon": "fas fa-rainbow" + }, + "txp2gene": { + "type": "string", + "description": "Specify a Kallisto gene mapping file here. If you don't, this will be automatically created in the Kallisto workflow when specifying a valid `--gtf` file.", + "fa_icon": "fas fa-rainbow" }, "kb_t1c": { "type": "string", "description": "Specify a path to the cDNA transcripts-to-capture.", - "fa_icon": "fas fa-fish" + "fa_icon": "fas fa-rainbow" }, "kb_t2c": { "type": "string", "description": "Specify a path to the intron transcripts-to-capture.", - "fa_icon": "fas fa-fish" + "fa_icon": "fas fa-rainbow" }, "kb_workflow": { "type": "string", "default": "standard", "description": "Type of workflow. Use `lamanno` for RNA velocity based on La Manno et al. 2018 logic. Use `nucleus` for RNA velocity on single-nucleus RNA-seq reads. Use `kite` for feature barcoding. Use `kite: 10xFB` for 10x Genomics Feature Barcoding technology. (default: standard)", - "fa_icon": "fas fa-fish", + "fa_icon": "fas fa-rainbow", "enum": ["standard", "lamanno", "nucleus", "kite", "kite: 10xFB"] } } From 752d051df605dbe192339e9446162882b4657d69 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Mon, 12 Feb 2024 13:31:59 -0800 Subject: [PATCH 026/111] Update nextflow_schema.json --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index b94b6460..56785f0e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -201,7 +201,7 @@ "type": "object", "description": "Params related to Kallisto/BUS tool", "default": "", - "fa_icon": "fas fa-fish", + "fa_icon": "fas fa-rainbow", "properties": { "kallisto_index": { "type": "string", From a2ef2270ce69e128d408c1d35765c26b924e4c3d Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Tue, 13 Feb 2024 08:19:36 +0100 Subject: [PATCH 027/111] Update modules --- modules.json | 16 ++-- modules/nf-core/cellranger/count/meta.yml | 4 +- .../count/templates/cellranger_count.py | 4 +- modules/nf-core/cellranger/mkgtf/meta.yml | 4 +- modules/nf-core/cellrangerarc/count/meta.yml | 2 +- modules/nf-core/cellrangerarc/mkgtf/meta.yml | 2 +- modules/nf-core/fastqc/tests/main.nf.test | 75 ++--------------- .../nf-core/fastqc/tests/main.nf.test.snap | 76 +++++++++++++++++- modules/nf-core/gffread/meta.yml | 4 +- modules/nf-core/gffread/tests/main.nf.test | 20 +++-- .../nf-core/gffread/tests/main.nf.test.snap | 56 ++++--------- modules/nf-core/multiqc/tests/main.nf.test | 13 +-- .../nf-core/multiqc/tests/main.nf.test.snap | 28 ++++++- .../star/genomegenerate/environment.yml | 3 +- .../star/genomegenerate/tests/main.nf.test | 40 +++++----- .../genomegenerate/tests/main.nf.test.snap | 80 +++++++++++++++++-- 16 files changed, 248 insertions(+), 179 deletions(-) diff --git a/modules.json b/modules.json index 94fd5c8b..c71a0499 100644 --- a/modules.json +++ b/modules.json @@ -7,12 +7,12 @@ "nf-core": { "cellranger/count": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", "installed_by": ["modules"] }, "cellranger/mkgtf": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", "installed_by": ["modules"] }, "cellranger/mkref": { @@ -22,12 +22,12 @@ }, "cellrangerarc/count": { "branch": "master", - "git_sha": "4196b1b2e7ce265892f3979eabf7a9ddc030702f", + "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", "installed_by": ["modules"] }, "cellrangerarc/mkgtf": { "branch": "master", - "git_sha": "4196b1b2e7ce265892f3979eabf7a9ddc030702f", + "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", "installed_by": ["modules"] }, "cellrangerarc/mkref": { @@ -42,12 +42,12 @@ }, "fastqc": { "branch": "master", - "git_sha": "617777a807a1770f73deb38c80004bac06807eef", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "gffread": { "branch": "master", - "git_sha": "b8858b10356b87db4325341872816f9672541b7b", + "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", "installed_by": ["modules"] }, "gunzip": { @@ -67,12 +67,12 @@ }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "9e71d8519dfbfc328c078bba14d4bd4c99e39a94", "installed_by": ["modules"] }, "star/genomegenerate": { "branch": "master", - "git_sha": "d87a6e2156c2099c09280fa70776eaf0a824817a", + "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9", "installed_by": ["modules"] }, "universc": { diff --git a/modules/nf-core/cellranger/count/meta.yml b/modules/nf-core/cellranger/count/meta.yml index a672180e..c1f4259a 100644 --- a/modules/nf-core/cellranger/count/meta.yml +++ b/modules/nf-core/cellranger/count/meta.yml @@ -45,7 +45,7 @@ output: pattern: "versions.yml" authors: - "@ggabernet" - - "@Emiller88" + - "@edmundmiller" maintainers: - "@ggabernet" - - "@Emiller88" + - "@edmundmiller" diff --git a/modules/nf-core/cellranger/count/templates/cellranger_count.py b/modules/nf-core/cellranger/count/templates/cellranger_count.py index 4bfb9f4f..1527ba76 100644 --- a/modules/nf-core/cellranger/count/templates/cellranger_count.py +++ b/modules/nf-core/cellranger/count/templates/cellranger_count.py @@ -34,11 +34,11 @@ def chunk_iter(seq, size): # Match R1 in the filename, but only if it is followed by a non-digit or non-character # match "file_R1.fastq.gz", "file.R1_000.fastq.gz", etc. but # do not match "SRR12345", "file_INFIXR12", etc -filename_pattern = r"([^a-zA-Z0-9])R1([^a-zA-Z0-9])" +filename_pattern = r'([^a-zA-Z0-9])R1([^a-zA-Z0-9])' for i, (r1, r2) in enumerate(chunk_iter(fastqs, 2)): # double escapes are required because nextflow processes this python 'template' - if re.sub(filename_pattern, r"\\1R2\\2", r1.name) != r2.name: + if re.sub(filename_pattern, r'\\1R2\\2', r1.name) != r2.name: raise AssertionError( dedent( f"""\ diff --git a/modules/nf-core/cellranger/mkgtf/meta.yml b/modules/nf-core/cellranger/mkgtf/meta.yml index 7ec0e0a3..a01e2830 100644 --- a/modules/nf-core/cellranger/mkgtf/meta.yml +++ b/modules/nf-core/cellranger/mkgtf/meta.yml @@ -27,7 +27,7 @@ output: pattern: "versions.yml" authors: - "@ggabernet" - - "@Emiller88" + - "@edmundmiller" maintainers: - "@ggabernet" - - "@Emiller88" + - "@edmundmiller" diff --git a/modules/nf-core/cellrangerarc/count/meta.yml b/modules/nf-core/cellrangerarc/count/meta.yml index 919de4dc..8bc73c6f 100644 --- a/modules/nf-core/cellrangerarc/count/meta.yml +++ b/modules/nf-core/cellrangerarc/count/meta.yml @@ -36,5 +36,5 @@ output: pattern: "versions.yml" authors: - "@ggabernet" - - "@Emiller88" + - "@edmundmiller" - "@heylf" diff --git a/modules/nf-core/cellrangerarc/mkgtf/meta.yml b/modules/nf-core/cellrangerarc/mkgtf/meta.yml index 923c3e18..5fadc3ef 100644 --- a/modules/nf-core/cellrangerarc/mkgtf/meta.yml +++ b/modules/nf-core/cellrangerarc/mkgtf/meta.yml @@ -28,5 +28,5 @@ output: pattern: "versions.yml" authors: - "@ggabernet" - - "@Emiller88" + - "@edmundmiller" - "@heylf" diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index acbcbe02..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -42,7 +42,6 @@ nextflow_process { when { process { -<<<<<<< HEAD """ input[0] = Channel.of([ [id: 'test', single_end: false], // meta map @@ -50,17 +49,6 @@ nextflow_process { file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_2.fastq.gz', checkIfExists: true) ] ]) """ -======= - """ - input[0] = [ - [id: 'test', single_end: false], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true) - ] - ] - """ ->>>>>>> dev } } @@ -75,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -84,19 +72,11 @@ nextflow_process { when { process { -<<<<<<< HEAD """ input[0] = Channel.of([ [id: 'test', single_end: false], // meta map file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_interleaved.fastq.gz', checkIfExists: true) ]) -======= - """ - input[0] = [ - [id: 'test', single_end: false], // meta map - file(params.test_data['sarscov2']['illumina']['test_interleaved_fastq_gz'], checkIfExists: true) - ] ->>>>>>> dev """ } } @@ -109,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -118,21 +98,12 @@ nextflow_process { when { process { -<<<<<<< HEAD """ input[0] = Channel.of([ [id: 'test', single_end: false], // meta map file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/bam/test.paired_end.sorted.bam', checkIfExists: true) ]) """ -======= - """ - input[0] = [ - [id: 'test', single_end: false], // meta map - file(params.test_data['sarscov2']['illumina']['test_paired_end_sorted_bam'], checkIfExists: true) - ] - """ ->>>>>>> dev } } @@ -144,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +124,6 @@ nextflow_process { when { process { -<<<<<<< HEAD """ input[0] = Channel.of([ [id: 'test', single_end: false], // meta map @@ -163,19 +133,6 @@ nextflow_process { file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test2_2.fastq.gz', checkIfExists: true) ] ]) """ -======= - """ - input[0] = [ - [id: 'test', single_end: false], // meta map - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test_2_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_1_fastq_gz'], checkIfExists: true), - file(params.test_data['sarscov2']['illumina']['test2_2_fastq_gz'], checkIfExists: true) - ] - ] - """ ->>>>>>> dev } } @@ -196,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -205,21 +162,12 @@ nextflow_process { when { process { -<<<<<<< HEAD """ input[0] = Channel.of([ [ id:'mysample', single_end:true ], // meta map file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ]) """ -======= - """ - input[0] = [ - [ id:'mysample', single_end:true ], // meta map - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - """ ->>>>>>> dev } } @@ -231,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -243,19 +191,10 @@ nextflow_process { when { process { """ -<<<<<<< HEAD input[0] = Channel.of([ [ id: 'test', single_end:true ], [ file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastq/test_1.fastq.gz', checkIfExists: true) ] ]) -======= - input[0] = [ - [ id: 'test', single_end:true ], - [ - file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz'], checkIfExists: true) - ] - ] ->>>>>>> dev """ } } @@ -265,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/gffread/meta.yml b/modules/nf-core/gffread/meta.yml index 27ac3105..d38cbcda 100644 --- a/modules/nf-core/gffread/meta.yml +++ b/modules/nf-core/gffread/meta.yml @@ -31,6 +31,6 @@ output: description: File containing software versions pattern: "versions.yml" authors: - - "@emiller88" + - "@edmundmiller" maintainers: - - "@emiller88" + - "@edmundmiller" diff --git a/modules/nf-core/gffread/tests/main.nf.test b/modules/nf-core/gffread/tests/main.nf.test index c4dfbdf4..bdbc96ae 100644 --- a/modules/nf-core/gffread/tests/main.nf.test +++ b/modules/nf-core/gffread/tests/main.nf.test @@ -25,10 +25,12 @@ nextflow_process { then { assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.gtf != null }, - { assert process.out.gffread_gff == [] } + { assert process.success }, + { assert snapshot( + process.out.gtf, + process.out.versions + ).match() }, + { assert process.out.gffread_gff == [] } ) } @@ -49,10 +51,12 @@ nextflow_process { then { assertAll ( - { assert process.success }, - { assert snapshot(process.out).match() }, - { assert process.out.gtf == [] }, - { assert process.out.gffread_gff != null }, + { assert process.success }, + { assert snapshot( + process.out.gffread_gff, + process.out.versions + ).match() }, + { assert process.out.gtf == [] }, ) } diff --git a/modules/nf-core/gffread/tests/main.nf.test.snap b/modules/nf-core/gffread/tests/main.nf.test.snap index 1f1342e1..00a11a40 100644 --- a/modules/nf-core/gffread/tests/main.nf.test.snap +++ b/modules/nf-core/gffread/tests/main.nf.test.snap @@ -1,52 +1,24 @@ { "sarscov2-gff3-gtf": { "content": [ - { - "0": [ - "genome.gtf:md5,2394072d7d31530dfd590c4a117bf6e3" - ], - "1": [ - - ], - "2": [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ], - "gffread_gff": [ - - ], - "gtf": [ - "genome.gtf:md5,2394072d7d31530dfd590c4a117bf6e3" - ], - "versions": [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ] - } + [ + "genome.gtf:md5,2394072d7d31530dfd590c4a117bf6e3" + ], + [ + "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" + ] ], - "timestamp": "2023-11-29T15:39:30.006985" + "timestamp": "2024-01-23T20:00:32.688779117" }, "sarscov2-gff3-gff3": { "content": [ - { - "0": [ - - ], - "1": [ - "genome.gffread.gff3:md5,a7d40d99dcddac23ac673c473279ea2d" - ], - "2": [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ], - "gffread_gff": [ - "genome.gffread.gff3:md5,a7d40d99dcddac23ac673c473279ea2d" - ], - "gtf": [ - - ], - "versions": [ - "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" - ] - } + [ + "genome.gffread.gff3:md5,a7d40d99dcddac23ac673c473279ea2d" + ], + [ + "versions.yml:md5,a71b6cdfa528dd206a238ec64bae13d6" + ] ], - "timestamp": "2023-11-29T15:39:34.636061" + "timestamp": "2024-01-23T20:07:11.457356625" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438eda..f1c4242e 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e7304..549ba79c 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,13 +1,17 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:43:40.529579" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", @@ -16,6 +20,22 @@ "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:45:09.605359" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:44:53.535994" } } \ No newline at end of file diff --git a/modules/nf-core/star/genomegenerate/environment.yml b/modules/nf-core/star/genomegenerate/environment.yml index 93e4476a..791f255e 100644 --- a/modules/nf-core/star/genomegenerate/environment.yml +++ b/modules/nf-core/star/genomegenerate/environment.yml @@ -1,11 +1,10 @@ name: star_genomegenerate - channels: - conda-forge - bioconda - defaults - dependencies: - bioconda::samtools=1.18 + - bioconda::htslib=1.18 - bioconda::star=2.7.10a - conda-forge::gawk=5.1.0 diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test b/modules/nf-core/star/genomegenerate/tests/main.nf.test index af0c9421..c17c8ba4 100644 --- a/modules/nf-core/star/genomegenerate/tests/main.nf.test +++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test @@ -8,18 +8,18 @@ nextflow_process { tag "star" tag "star/genomegenerate" - test("homo_sapiens") { + test("fasta_gtf") { when { process { """ input[0] = Channel.of([ [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] ]) input[1] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) """ } @@ -28,14 +28,13 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("index_with_gtf") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_gtf_index") }, + { assert snapshot(process.out.versions).match("fasta_gtf_versions") } ) } - } - test("homo_sapiens-stub") { + test("fasta_gtf_stub") { options '-stub' @@ -44,11 +43,11 @@ nextflow_process { """ input[0] = Channel.of([ [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] ]) input[1] = Channel.of([ [ id:'test_gtf' ], - [file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) ] ]) """ } @@ -57,21 +56,20 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("index_with_gtf") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_gtf_stub_index") }, + { assert snapshot(process.out.versions).match("fasta_gtf_stub_versions") } ) } - } - test("homo_sapiens-without_gtf") { + test("fasta") { when { process { """ input[0] = Channel.of([ [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] ]) input[1] = Channel.of([ [], [] ]) """ @@ -81,14 +79,14 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("index_without_gtf") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_index") }, + { assert snapshot(process.out.versions).match("fasta_versions") } ) } } - test("homo_sapiens-without_gtf-stub") { + test("fasta_stub") { options '-stub' @@ -97,7 +95,7 @@ nextflow_process { """ input[0] = Channel.of([ [ id:'test_fasta' ], - [file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true)] + [ file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) ] ]) input[1] = Channel.of([ [], [] ]) """ @@ -107,11 +105,11 @@ nextflow_process { then { assertAll( { assert process.success }, - { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("index_without_gtf") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(file(process.out.index[0][1]).listFiles().collect { it.getName() }.sort().toString()).match("fasta_stub_index") }, + { assert snapshot(process.out.versions).match("fasta_stub_versions") } ) } } -} \ No newline at end of file +} diff --git a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap index 9de08c74..5653d6e6 100644 --- a/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap +++ b/modules/nf-core/star/genomegenerate/tests/main.nf.test.snap @@ -1,22 +1,90 @@ { - "versions": { + "fasta_gtf_versions": { "content": [ [ "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f" ] ], - "timestamp": "2023-12-19T11:05:51.741109" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:31.798555" }, - "index_with_gtf": { + "fasta_stub_versions": { + "content": [ + [ + "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:55:07.521209" + }, + "fasta_gtf_stub_index": { + "content": [ + "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:46.478098" + }, + "fasta_gtf_stub_versions": { + "content": [ + [ + "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:46.491657" + }, + "fasta_index": { + "content": [ + "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:57.552329" + }, + "fasta_versions": { + "content": [ + [ + "versions.yml:md5,46b8f1f34bb7f23892cd1eb249ed4d7f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:57.560541" + }, + "fasta_gtf_index": { "content": [ "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, exonGeTrInfo.tab, exonInfo.tab, geneInfo.tab, genomeParameters.txt, sjdbInfo.txt, sjdbList.fromGTF.out.tab, sjdbList.out.tab, transcriptInfo.tab]" ], - "timestamp": "2023-12-19T11:38:14.551548" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:54:31.786814" }, - "index_without_gtf": { + "fasta_stub_index": { "content": [ "[Genome, Log.out, SA, SAindex, chrLength.txt, chrName.txt, chrNameLength.txt, chrStart.txt, genomeParameters.txt]" ], - "timestamp": "2023-12-19T11:38:22.382905" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.04.3" + }, + "timestamp": "2024-02-01T15:55:07.517472" } } \ No newline at end of file From c14ac9c4f0145d5b643545dab0ac9640eca441d7 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Tue, 13 Feb 2024 08:27:41 +0100 Subject: [PATCH 028/111] Fix pre-commit --- .devcontainer/devcontainer.json | 8 ++-- .github/workflows/awstest.yml | 4 -- .github/workflows/download_pipeline.yml | 2 +- subworkflows/local/align_cellrangerarc.nf | 6 +-- subworkflows/local/align_universc.nf | 12 +++--- subworkflows/local/fastqc.nf | 50 +++++++++++------------ subworkflows/local/input_check.nf | 6 +-- 7 files changed, 43 insertions(+), 45 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4a9bc5c7..4ecfbfe3 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -18,11 +18,11 @@ "python.linting.flake8Path": "/opt/conda/bin/flake8", "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint", + "python.linting.pylintPath": "/opt/conda/bin/pylint" }, // Add the IDs of extensions you want installed when the container is created. - "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"], - }, - }, + "extensions": ["ms-python.python", "ms-python.vscode-pylance", "nf-core.nf-core-extensionpack"] + } + } } diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 9178d307..ab0d7d3c 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -28,12 +28,8 @@ jobs: "aligner": "${{ matrix.aligner }}" } profiles: test -<<<<<<< HEAD - uses: actions/upload-artifact@v4 -======= - - uses: actions/upload-artifact@v3 ->>>>>>> dev with: name: Tower debug log file path: | diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..8a330045 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -64,4 +64,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/subworkflows/local/align_cellrangerarc.nf b/subworkflows/local/align_cellrangerarc.nf index 3232a020..6de84e7b 100644 --- a/subworkflows/local/align_cellrangerarc.nf +++ b/subworkflows/local/align_cellrangerarc.nf @@ -29,8 +29,8 @@ workflow CELLRANGERARC_ALIGN { ch_versions = ch_versions.mix(CELLRANGERARC_MKGTF.out.versions) // Make reference genome - assert ( ( !params.cellrangerarc_reference && !cellrangerarc_config ) || - ( params.cellrangerarc_reference && cellrangerarc_config ) ) : + assert (( !params.cellrangerarc_reference && !cellrangerarc_config ) || + ( params.cellrangerarc_reference && cellrangerarc_config ) ) : "If you provide a config file you also have to specific the reference name and vice versa." cellrangerarc_reference = 'cellrangerarc_reference' @@ -53,4 +53,4 @@ workflow CELLRANGERARC_ALIGN { emit: ch_versions cellranger_arc_out = CELLRANGERARC_COUNT.out.outs -} \ No newline at end of file +} diff --git a/subworkflows/local/align_universc.nf b/subworkflows/local/align_universc.nf index acb2c560..cf16985e 100644 --- a/subworkflows/local/align_universc.nf +++ b/subworkflows/local/align_universc.nf @@ -34,11 +34,13 @@ workflow UNIVERSC_ALIGN { // Obtain read counts UNIVERSC ( - ch_fastq.map{ meta, reads -> [ - // defaults - ["samples": [meta.id], "technology": universc_technology, "chemistry": "auto", "single_end": false, "strandedness": "forward"] + meta, // + meta overrides defaults with information already in meta - reads - ] }, + ch_fastq.map{ + meta, reads -> [ + // defaults + ["samples": [meta.id], "technology": universc_technology, "chemistry": "auto", "single_end": false, "strandedness": "forward"] + meta, // + meta overrides defaults with information already in meta + reads + ] + }, universc_index ) ch_versions = ch_versions.mix(UNIVERSC.out.versions) diff --git a/subworkflows/local/fastqc.nf b/subworkflows/local/fastqc.nf index 6825a9e0..05bb128b 100644 --- a/subworkflows/local/fastqc.nf +++ b/subworkflows/local/fastqc.nf @@ -4,35 +4,35 @@ include { FASTQC } from '../../modules/nf-core/fastqc/main' workflow FASTQC_CHECK { - take: - ch_fastq + take: + ch_fastq - main: + main: - def n = (params.aligner == 'cellrangerarc') ? 3 : 1 - ch_fastq.map { ch -> [ ch[0], ch[n] ] }.set { ch_fastq } + def n = (params.aligner == 'cellrangerarc') ? 3 : 1 + ch_fastq.map { ch -> [ ch[0], ch[n] ] }.set { ch_fastq } - /* - * FastQ QC using FASTQC - */ - FASTQC ( ch_fastq ) - fastqc_zip = FASTQC.out.zip - fastqc_html = FASTQC.out.html + /* + * FastQ QC using FASTQC + */ + FASTQC ( ch_fastq ) + fastqc_zip = FASTQC.out.zip + fastqc_html = FASTQC.out.html - fastqc_zip - .map { it -> [ it[1] ] } - .set { fastqc_zip_only } - fastqc_html - .map { it -> [ it[1] ] } - .set { fastqc_html_only } + fastqc_zip + .map { it -> [ it[1] ] } + .set { fastqc_zip_only } + fastqc_html + .map { it -> [ it[1] ] } + .set { fastqc_html_only } - fastqc_multiqc = Channel.empty() - fastqc_multiqc = fastqc_multiqc.mix( fastqc_zip_only, fastqc_html_only ) - fastqc_version = FASTQC.out.versions + fastqc_multiqc = Channel.empty() + fastqc_multiqc = fastqc_multiqc.mix( fastqc_zip_only, fastqc_html_only ) + fastqc_version = FASTQC.out.versions - emit: - fastqc_zip - fastqc_html - fastqc_version - fastqc_multiqc + emit: + fastqc_zip + fastqc_html + fastqc_version + fastqc_multiqc } diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf index 2e06e889..48849fc9 100644 --- a/subworkflows/local/input_check.nf +++ b/subworkflows/local/input_check.nf @@ -24,11 +24,11 @@ workflow INPUT_CHECK { if (params.aligner == 'cellrangerarc' ) { grouped_ch - .map { meta, sample_type, sub_sample, reads -> [ meta, sample_type.flatten(), sub_sample.flatten(), reads.flatten() ] } + .map { meta, sample_type, sub_sample, reads -> [ meta, sample_type.flatten(), sub_sample.flatten(), reads.flatten() ] } .set { reads } } else { grouped_ch - .map { meta, reads -> [ meta, reads.flatten() ] } + .map { meta, reads -> [ meta, reads.flatten() ] } .set { reads } } @@ -93,4 +93,4 @@ def create_fastq_channel(LinkedHashMap row) { } return fastq_meta -} \ No newline at end of file +} From 514367e11fa94dbbc9bda271a5b52b974759743f Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Tue, 13 Feb 2024 08:28:56 +0100 Subject: [PATCH 029/111] Fix lint --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 34af4c64..a53cc55e 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -135,7 +135,7 @@ "type": "string", "format": "directory-path", "description": "Directory / URL base for iGenomes references.", - "default": "s3://ngi-igenomes/igenomes", + "default": "s3://ngi-igenomes/igenomes/", "fa_icon": "fas fa-cloud-download-alt", "hidden": true } From 3a89373a5594d674e807e7936fb72ab76ecdb2ab Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 13 Feb 2024 11:01:17 +0000 Subject: [PATCH 030/111] update tests --- tests/main_pipeline_alevin.test | 31 ++++++++++------------- tests/main_pipeline_cellranger.test | 39 ++++++++++------------------- tests/main_pipeline_kallisto.test | 33 +++++++++++------------- tests/main_pipeline_star.test | 37 ++++++++------------------- 4 files changed, 50 insertions(+), 90 deletions(-) diff --git a/tests/main_pipeline_alevin.test b/tests/main_pipeline_alevin.test index c51a8ee9..f2730699 100644 --- a/tests/main_pipeline_alevin.test +++ b/tests/main_pipeline_alevin.test @@ -24,30 +24,25 @@ nextflow_pipeline { // General assertions // - // Did it finish successfully? - assert workflow.success + // Did it finish successfully? + assert workflow.success - // How many tasks were executed? - assert workflow.trace.tasks().size() == 16 + // How many tasks were executed? + assert workflow.trace.tasks().size() == 16 - // How many results were produced? - assert path("${outputDir}/results_alevin").list().size() == 5 - assert path("${outputDir}/results_alevin/alevin").list().size() == 4 - assert path("${outputDir}/results_alevin/alevin/mtx_conversions").list().size() == 4 - assert path("${outputDir}/results_alevin/alevinqc").list().size() == 2 - assert path("${outputDir}/results_alevin/fastqc").list().size() == 12 - assert path("${outputDir}/results_alevin/multiqc").list().size() == 3 + // How many results were produced? + assert path("${outputDir}/results_alevin").list().size() == 5 + assert path("${outputDir}/results_alevin/alevin").list().size() == 4 + assert path("${outputDir}/results_alevin/alevin/mtx_conversions").list().size() == 4 + assert path("${outputDir}/results_alevin/alevinqc").list().size() == 2 + assert path("${outputDir}/results_alevin/fastqc").list().size() == 12 + assert path("${outputDir}/results_alevin/multiqc").list().size() == 3 // // Check if files were produced // - assert new File( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_cols.txt" ).exists() - assert new File( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat.mtx" ).exists() - assert new File( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_rows.txt" ).exists() - - assert new File( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_cols.txt" ).exists() - assert new File( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat.mtx" ).exists() - assert new File( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_rows.txt" ).exists() + assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() + assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() // // Check if files are the same diff --git a/tests/main_pipeline_cellranger.test b/tests/main_pipeline_cellranger.test index c90436f6..314177e0 100644 --- a/tests/main_pipeline_cellranger.test +++ b/tests/main_pipeline_cellranger.test @@ -24,38 +24,25 @@ nextflow_pipeline { // General assertions // - // Did it finish successfully? - assert workflow.success + // Did it finish successfully? + assert workflow.success - // How many tasks were executed? - assert workflow.trace.tasks().size() == 15 + // How many tasks were executed? + assert workflow.trace.tasks().size() == 15 - // How many results were produced? - assert path("${outputDir}/results_cellranger").list().size() == 4 - assert path("${outputDir}/results_cellranger/cellranger").list().size() == 4 - assert path("${outputDir}/results_cellranger/cellranger/mtx_conversions").list().size() == 4 - assert path("${outputDir}/results_cellranger/cellranger/count").list().size() == 3 - assert path("${outputDir}/results_cellranger/fastqc").list().size() == 12 - assert path("${outputDir}/results_cellranger/multiqc").list().size() == 3 + // How many results were produced? + assert path("${outputDir}/results_cellranger").list().size() == 4 + assert path("${outputDir}/results_cellranger/cellranger").list().size() == 4 + assert path("${outputDir}/results_cellranger/cellranger/mtx_conversions").list().size() == 4 + assert path("${outputDir}/results_cellranger/cellranger/count").list().size() == 3 + assert path("${outputDir}/results_cellranger/fastqc").list().size() == 12 + assert path("${outputDir}/results_cellranger/multiqc").list().size() == 3 // // Check if files were produced // - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/features.tsv.gz" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ).exists() - - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/features.tsv.gz" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ).exists() - - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/features.tsv.gz" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/matrix.mtx.gz" ).exists() - - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/features.tsv.gz" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/matrix.mtx.gz" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() + assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() // // Check if files are the same diff --git a/tests/main_pipeline_kallisto.test b/tests/main_pipeline_kallisto.test index 3bae1f31..1b06f249 100644 --- a/tests/main_pipeline_kallisto.test +++ b/tests/main_pipeline_kallisto.test @@ -24,31 +24,26 @@ nextflow_pipeline { // General assertions // - // Did it finish successfully? - assert workflow.success + // Did it finish successfully? + assert workflow.success - // How many tasks were executed? - assert workflow.trace.tasks().size() == 14 + // How many tasks were executed? + assert workflow.trace.tasks().size() == 14 - // How many results were produced? - assert path("${outputDir}/results_kallisto").list().size() == 4 - assert path("${outputDir}/results_kallisto/kallisto").list().size() == 4 - assert path("${outputDir}/results_kallisto/kallisto/mtx_conversions").list().size() == 4 - assert path("${outputDir}/results_kallisto/kallisto/Sample_X.count").list().size() == 9 - assert path("${outputDir}/results_kallisto/kallisto/Sample_Y.count").list().size() == 9 - assert path("${outputDir}/results_kallisto/fastqc").list().size() == 12 - assert path("${outputDir}/results_kallisto/multiqc").list().size() == 3 + // How many results were produced? + assert path("${outputDir}/results_kallisto").list().size() == 4 + assert path("${outputDir}/results_kallisto/kallisto").list().size() == 4 + assert path("${outputDir}/results_kallisto/kallisto/mtx_conversions").list().size() == 4 + assert path("${outputDir}/results_kallisto/kallisto/Sample_X.count").list().size() == 9 + assert path("${outputDir}/results_kallisto/kallisto/Sample_Y.count").list().size() == 9 + assert path("${outputDir}/results_kallisto/fastqc").list().size() == 12 + assert path("${outputDir}/results_kallisto/multiqc").list().size() == 3 // // Check if files were produced // - assert new File( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.barcodes.txt" ).exists() - assert new File( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.genes.txt" ).exists() - assert new File( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.mtx" ).exists() - - assert new File( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.barcodes.txt" ).exists() - assert new File( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.genes.txt" ).exists() - assert new File( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.mtx" ).exists() + assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() + assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() // // Check if files are the same diff --git a/tests/main_pipeline_star.test b/tests/main_pipeline_star.test index d20657f1..de07c8d3 100644 --- a/tests/main_pipeline_star.test +++ b/tests/main_pipeline_star.test @@ -24,41 +24,24 @@ nextflow_pipeline { // General assertions // - // Did it finish successfully? - assert workflow.success + // Did it finish successfully? + assert workflow.success - // How many tasks were executed? - assert workflow.trace.tasks().size() == 14 + // How many tasks were executed? + assert workflow.trace.tasks().size() == 14 - // How many results were produced? - assert path("${outputDir}/results_star").list().size() == 4 - assert path("${outputDir}/results_star/star").list().size() == 3 - assert path("${outputDir}/results_star/star/mtx_conversions").list().size() == 4 - assert path("${outputDir}/results_star/fastqc").list().size() == 12 - assert path("${outputDir}/results_star/multiqc").list().size() == 3 + // How many results were produced? + assert path("${outputDir}/results_star").list().size() == 4 + assert path("${outputDir}/results_star/star").list().size() == 3 + assert path("${outputDir}/results_star/star/mtx_conversions").list().size() == 4 + assert path("${outputDir}/results_star/fastqc").list().size() == 12 + assert path("${outputDir}/results_star/multiqc").list().size() == 3 // // Check if files were produced // - // - // Check if main files exist - // - - assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.SJ.out.tab" ).exists() - assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Barcodes.stats" ).exists() - assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/matrix.mtx.gz" ).exists() - assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/features.tsv.gz" ).exists() - assert new File( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/barcodes.tsv.gz" ).exists() assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() - assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.rds" ).exists() - - assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.SJ.out.tab" ).exists() - assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Barcodes.stats" ).exists() - assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/matrix.mtx.gz" ).exists() - assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/features.tsv.gz" ).exists() - assert new File( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/barcodes.tsv.gz" ).exists() assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() - assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ).exists() // // Check if files are the same From ce0db16615336546d6683f5ec4da36b0b2316edb Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 13 Feb 2024 11:15:15 +0000 Subject: [PATCH 031/111] update ci.yml for nf-test --- .github/workflows/ci.yml | 68 +++++++++++++++++++++++++++++----------- 1 file changed, 49 insertions(+), 19 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index e8907caf..574c5fd3 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -1,39 +1,43 @@ -name: nf-core CI # This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors +name: nf-core CI on: - push: - branches: - - dev pull_request: release: types: [published] + merge_group: + types: + - checks_requested + branches: + - master + - dev env: NXF_ANSI_LOG: false + NFTEST_VER: "0.8.1" concurrency: - group: ${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }} + group: "${{ github.workflow }}-${{ github.event.pull_request.number || github.ref }}" cancel-in-progress: true jobs: test: - name: Run pipeline with test data - # Only run on push if this is the nf-core dev branch (merged PRs) - if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/scrnaseq') }}" + name: ${{ matrix.profile }} NF ${{ matrix.NXF_VER }} + needs: [changes, define_nxf_versions] runs-on: ubuntu-latest strategy: + fail-fast: false matrix: NXF_VER: - "23.04.0" - "latest-everything" - profile: [ - "test,docker --aligner alevin", - "test,docker --aligner kallisto", - "test,docker --aligner star", - "test,docker --aligner cellranger", - # "test,docker --aligner cellrangerarc", // this currently lacks a suitable test profile, see issue https://github.com/nf-core/scrnaseq/issues/290 - # "test,docker --aligner universc", // this is broken, see issue https://github.com/nf-core/scrnaseq/issues/289 + profile: + [ + "tests/main_pipeline_alevin.test", + "tests/main_pipeline_cellranger.test", + "tests/main_pipeline_kallisto.test", + "tests/main_pipeline_star.test", ] + steps: - name: Free some space run: | @@ -41,6 +45,7 @@ jobs: sudo rm -rf /opt/ghc sudo rm -rf "/usr/local/share/boost" sudo rm -rf "$AGENT_TOOLSDIRECTORY" + - name: Check out pipeline code uses: actions/checkout@v4 @@ -49,8 +54,33 @@ jobs: with: version: "${{ matrix.NXF_VER }}" - - name: Run pipeline with test data - # For example: adding multiple test runs with different parameters - # Remember that you can parallelise this by using strategy.matrix + - name: Cache nf-test installation + id: cache-software + uses: actions/cache@v3 + with: + path: | + /usr/local/bin/nf-test + /home/runner/.nf-test/nf-test.jar + key: ${{ runner.os }}-${{ env.NFTEST_VER }}-nftest + + - name: Install nf-test + if: steps.cache-software.outputs.cache-hit != 'true' + run: | + wget -qO- https://code.askimed.com/install/nf-test | bash + sudo mv nf-test /usr/local/bin/ + + - name: Run nf-test run: | - nextflow run ${GITHUB_WORKSPACE} -profile ${{ matrix.profile }} --outdir ./results + nf-test test "${{ matrix.profile }}" --junitxml=test.xml + + - name: Output log on failure + if: failure() + run: | + sudo apt install bat > /dev/null + batcat --decorations=always --color=always ${{ github.workspace }}/.nf-test/tests/*/meta/nextflow.log + + - name: Publish Test Report + uses: mikepenz/action-junit-report@v3 + if: always() # always run even if the previous step fails + with: + report_paths: test.xml From 4df4fbfce20b91b868518b267e2dce9d68e78f35 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 13 Feb 2024 11:16:37 +0000 Subject: [PATCH 032/111] also allow on push --- .github/workflows/ci.yml | 3 +++ 1 file changed, 3 insertions(+) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 574c5fd3..ddecad72 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -1,6 +1,9 @@ # This workflow runs the pipeline with the minimal test dataset to check that it completes without any syntax errors name: nf-core CI on: + push: + branches: + - dev pull_request: release: types: [published] From ae786d60635bf082c5d3fd4fa3c6326167e03460 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 13 Feb 2024 11:18:20 +0000 Subject: [PATCH 033/111] add if and remove needs --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index ddecad72..8413df7e 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -25,7 +25,7 @@ concurrency: jobs: test: name: ${{ matrix.profile }} NF ${{ matrix.NXF_VER }} - needs: [changes, define_nxf_versions] + if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/scrnaseq') }}" runs-on: ubuntu-latest strategy: fail-fast: false From e806454af392db3bd518c506c21674ebc2202ec7 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 13 Feb 2024 11:20:40 +0000 Subject: [PATCH 034/111] modify names to have only aligner name --- .github/workflows/ci.yml | 12 +++--------- 1 file changed, 3 insertions(+), 9 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 8413df7e..16953daa 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -24,7 +24,7 @@ concurrency: jobs: test: - name: ${{ matrix.profile }} NF ${{ matrix.NXF_VER }} + name: "aligner: ${{ matrix.profile }} ; NF: ${{ matrix.NXF_VER }}" if: "${{ github.event_name != 'push' || (github.event_name == 'push' && github.repository == 'nf-core/scrnaseq') }}" runs-on: ubuntu-latest strategy: @@ -33,13 +33,7 @@ jobs: NXF_VER: - "23.04.0" - "latest-everything" - profile: - [ - "tests/main_pipeline_alevin.test", - "tests/main_pipeline_cellranger.test", - "tests/main_pipeline_kallisto.test", - "tests/main_pipeline_star.test", - ] + profile: ["alevin", "cellranger", "kallisto", "star"] steps: - name: Free some space @@ -74,7 +68,7 @@ jobs: - name: Run nf-test run: | - nf-test test "${{ matrix.profile }}" --junitxml=test.xml + nf-test test tests/main_pipeline_"${{ matrix.profile }}".test --junitxml=test.xml - name: Output log on failure if: failure() From a2aeea40f2688c2b516ef2f49adc8f1505388c74 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 13 Feb 2024 12:02:35 +0000 Subject: [PATCH 035/111] fix quotation --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 16953daa..ea9587e5 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -68,7 +68,7 @@ jobs: - name: Run nf-test run: | - nf-test test tests/main_pipeline_"${{ matrix.profile }}".test --junitxml=test.xml + "nf-test test tests/main_pipeline_${{ matrix.profile }}.test --junitxml=test.xml" - name: Output log on failure if: failure() From 8684445e6d2233b2117cfcd7894b862388312323 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 13 Feb 2024 12:03:50 +0000 Subject: [PATCH 036/111] removing quotations --- .github/workflows/ci.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index ea9587e5..36d997da 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -68,7 +68,7 @@ jobs: - name: Run nf-test run: | - "nf-test test tests/main_pipeline_${{ matrix.profile }}.test --junitxml=test.xml" + nf-test test tests/main_pipeline_${{ matrix.profile }}.test --junitxml=test.xml - name: Output log on failure if: failure() From 4d90d609f1ececc9421619c572b8aa8a717ab37f Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 13 Feb 2024 10:03:11 -0800 Subject: [PATCH 037/111] de-duplicating txp2gene args (lint complains) --- nextflow_schema.json | 18 ++++++------------ 1 file changed, 6 insertions(+), 12 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 56785f0e..0dc716b1 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -138,7 +138,12 @@ "default": "s3://ngi-igenomes/igenomes", "fa_icon": "fas fa-cloud-download-alt", "hidden": true - } + }, + "txp2gene": { + "type": "string", + "description": "Kallisto or Alevin gene mapping file.", + "fa_icon": "fas map-marked-alt" + }, } }, "alevin_options": { @@ -152,12 +157,6 @@ "description": "This can be used to specify a precomputed Salmon index in the pipeline, in order to skip the generation of required indices by Salmon itself.", "fa_icon": "fas fa-fish" }, - "txp2gene": { - "type": "string", - "description": "Path to transcript to gene mapping file. This allows the specification of a transcript to gene mapping file for Salmon Alevin and AlevinQC.", - "help_text": "> This is the same as the `kallisto_gene_map` parameter below.", - "fa_icon": "fas fa-map-marked-alt" - }, "simpleaf_rlen": { "type": "integer", "default": 91, @@ -208,11 +207,6 @@ "description": "Specify a path to the precomputed Kallisto index.", "fa_icon": "fas fa-rainbow" }, - "txp2gene": { - "type": "string", - "description": "Specify a Kallisto gene mapping file here. If you don't, this will be automatically created in the Kallisto workflow when specifying a valid `--gtf` file.", - "fa_icon": "fas fa-rainbow" - }, "kb_t1c": { "type": "string", "description": "Specify a path to the cDNA transcripts-to-capture.", From efb1487d0a6bf6037c789121eeacf63173ba680c Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 13 Feb 2024 10:05:39 -0800 Subject: [PATCH 038/111] Update nextflow_schema.json --- nextflow_schema.json | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 0dc716b1..837ba7a0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -143,7 +143,7 @@ "type": "string", "description": "Kallisto or Alevin gene mapping file.", "fa_icon": "fas map-marked-alt" - }, + } } }, "alevin_options": { From 5ffdc888b8f31e53e7aae1f4d633bb70634a6785 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 13 Feb 2024 10:22:45 -0800 Subject: [PATCH 039/111] txp2gene has to be moved if it's shared --- nextflow.config | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index 9c3ac851..a774d835 100644 --- a/nextflow.config +++ b/nextflow.config @@ -19,15 +19,14 @@ params { // reference files genome = null transcript_fasta = null + txp2gene = null // salmon alevin parameters (simpleaf) simpleaf_rlen = 91 barcode_whitelist = null - txp2gene = null salmon_index = null // kallisto bustools parameters - kallisto_gene_map = null kallisto_index = null kb_workflow = "standard" kb_t1c = null From 38344a5018c0eaffdd6d7552dcb44b98d5133227 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 13 Feb 2024 10:44:28 -0800 Subject: [PATCH 040/111] adding explicit t1c and t2c arguments for kb Maybe it is possible to somehow inject them with ext.args, but this is a fairly standard pipeline option, and should probably be exposed. --- workflows/scrnaseq.nf | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 25740a8e..63381a6f 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -98,6 +98,8 @@ if (params.barcode_whitelist) { //kallisto params ch_kallisto_index = params.kallisto_index ? file(params.kallisto_index) : [] kb_workflow = params.kb_workflow +kb_t1c = params.kb_t1c ? file(params.kb_t1c) : [] +kb_t2c = params.kb_t2c ? file(params.kb_t2c) : [] //salmon params ch_salmon_index = params.salmon_index ? file(params.salmon_index) : [] @@ -144,6 +146,8 @@ workflow SCRNASEQ { ch_filter_gtf, ch_kallisto_index, ch_txp2gene, + kb_t1c, + kb_t2c, protocol_config['protocol'], kb_workflow, ch_fastq From da1eadeab233fc4500dcc5ebb2d34b7f9b9d7966 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Wed, 14 Feb 2024 08:15:48 +0000 Subject: [PATCH 041/111] updated tests to use assertAll() --- tests/main_pipeline_alevin.test | 76 +++++++++++++------------- tests/main_pipeline_cellranger.test | 82 +++++++++++++++-------------- tests/main_pipeline_kallisto.test | 72 +++++++++++++------------ tests/main_pipeline_star.test | 80 +++++++++++++++------------- 4 files changed, 163 insertions(+), 147 deletions(-) diff --git a/tests/main_pipeline_alevin.test b/tests/main_pipeline_alevin.test index f2730699..303a625b 100644 --- a/tests/main_pipeline_alevin.test +++ b/tests/main_pipeline_alevin.test @@ -20,42 +20,46 @@ nextflow_pipeline { then { - // - // General assertions - // - - // Did it finish successfully? - assert workflow.success - - // How many tasks were executed? - assert workflow.trace.tasks().size() == 16 - - // How many results were produced? - assert path("${outputDir}/results_alevin").list().size() == 5 - assert path("${outputDir}/results_alevin/alevin").list().size() == 4 - assert path("${outputDir}/results_alevin/alevin/mtx_conversions").list().size() == 4 - assert path("${outputDir}/results_alevin/alevinqc").list().size() == 2 - assert path("${outputDir}/results_alevin/fastqc").list().size() == 12 - assert path("${outputDir}/results_alevin/multiqc").list().size() == 3 - - // - // Check if files were produced - // - assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() - assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() - - // - // Check if files are the same - // - assert snapshot( - workflow, - path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_cols.txt" ), - path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat.mtx" ), - path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_rows.txt" ), - path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_cols.txt" ), - path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat.mtx" ), - path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_rows.txt" ) - ).match() + assertAll( + + // + // General assertions + // + + // Did it finish successfully? + {assert workflow.success}, + + // How many tasks were executed? + {assert workflow.trace.tasks().size() == 16}, + + // How many results were produced? + {assert path("${outputDir}/results_alevin").list().size() == 5}, + {assert path("${outputDir}/results_alevin/alevin").list().size() == 4}, + {assert path("${outputDir}/results_alevin/alevin/mtx_conversions").list().size() == 4}, + {assert path("${outputDir}/results_alevin/alevinqc").list().size() == 2}, + {assert path("${outputDir}/results_alevin/fastqc").list().size() == 12}, + {assert path("${outputDir}/results_alevin/multiqc").list().size() == 3}, + + // + // Check if files were produced + // + {assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists()}, + + // + // Check if files are the same + // + {assert snapshot( + workflow, + path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_cols.txt" ), + path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat.mtx" ), + path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_rows.txt" ), + path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_cols.txt" ), + path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat.mtx" ), + path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_rows.txt" ) + ).match()} + + ) // end of assertAll() } } diff --git a/tests/main_pipeline_cellranger.test b/tests/main_pipeline_cellranger.test index 314177e0..996c0f76 100644 --- a/tests/main_pipeline_cellranger.test +++ b/tests/main_pipeline_cellranger.test @@ -20,50 +20,54 @@ nextflow_pipeline { then { - // - // General assertions - // + assertAll( - // Did it finish successfully? - assert workflow.success + // + // General assertions + // - // How many tasks were executed? - assert workflow.trace.tasks().size() == 15 + // Did it finish successfully? + {assert workflow.success}, - // How many results were produced? - assert path("${outputDir}/results_cellranger").list().size() == 4 - assert path("${outputDir}/results_cellranger/cellranger").list().size() == 4 - assert path("${outputDir}/results_cellranger/cellranger/mtx_conversions").list().size() == 4 - assert path("${outputDir}/results_cellranger/cellranger/count").list().size() == 3 - assert path("${outputDir}/results_cellranger/fastqc").list().size() == 12 - assert path("${outputDir}/results_cellranger/multiqc").list().size() == 3 + // How many tasks were executed? + {assert workflow.trace.tasks().size() == 15}, - // - // Check if files were produced - // - assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() - assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() + // How many results were produced? + {assert path("${outputDir}/results_cellranger").list().size() == 4}, + {assert path("${outputDir}/results_cellranger/cellranger").list().size() == 4}, + {assert path("${outputDir}/results_cellranger/cellranger/mtx_conversions").list().size() == 4}, + {assert path("${outputDir}/results_cellranger/cellranger/count").list().size() == 3}, + {assert path("${outputDir}/results_cellranger/fastqc").list().size() == 12}, + {assert path("${outputDir}/results_cellranger/multiqc").list().size() == 3}, - // - // Check if files are the same - // - assert snapshot( - workflow, - path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/features.tsv.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/features.tsv.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/features.tsv.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/matrix.mtx.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/features.tsv.gz" ), - path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/matrix.mtx.gz" ), - path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), - path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ) - ).match() + // + // Check if files were produced + // + {assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists()}, + + // + // Check if files are the same + // + {assert snapshot( + workflow, + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/features.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/barcodes.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/features.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/filtered_feature_bc_matrix/matrix.mtx.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/features.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_X/outs/raw_feature_bc_matrix/matrix.mtx.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/features.tsv.gz" ), + path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/matrix.mtx.gz" ), + path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), + path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ) + ).match()} + + ) // end of assertAll() } } diff --git a/tests/main_pipeline_kallisto.test b/tests/main_pipeline_kallisto.test index 1b06f249..98ede8c4 100644 --- a/tests/main_pipeline_kallisto.test +++ b/tests/main_pipeline_kallisto.test @@ -20,45 +20,49 @@ nextflow_pipeline { then { - // - // General assertions - // + assertAll( - // Did it finish successfully? - assert workflow.success + // + // General assertions + // - // How many tasks were executed? - assert workflow.trace.tasks().size() == 14 + // Did it finish successfully? + {assert workflow.success}, - // How many results were produced? - assert path("${outputDir}/results_kallisto").list().size() == 4 - assert path("${outputDir}/results_kallisto/kallisto").list().size() == 4 - assert path("${outputDir}/results_kallisto/kallisto/mtx_conversions").list().size() == 4 - assert path("${outputDir}/results_kallisto/kallisto/Sample_X.count").list().size() == 9 - assert path("${outputDir}/results_kallisto/kallisto/Sample_Y.count").list().size() == 9 - assert path("${outputDir}/results_kallisto/fastqc").list().size() == 12 - assert path("${outputDir}/results_kallisto/multiqc").list().size() == 3 + // How many tasks were executed? + {assert workflow.trace.tasks().size() == 14}, - // - // Check if files were produced - // - assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() - assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() + // How many results were produced? + {assert path("${outputDir}/results_kallisto").list().size() == 4}, + {assert path("${outputDir}/results_kallisto/kallisto").list().size() == 4}, + {assert path("${outputDir}/results_kallisto/kallisto/mtx_conversions").list().size() == 4}, + {assert path("${outputDir}/results_kallisto/kallisto/Sample_X.count").list().size() == 9}, + {assert path("${outputDir}/results_kallisto/kallisto/Sample_Y.count").list().size() == 9}, + {assert path("${outputDir}/results_kallisto/fastqc").list().size() == 12}, + {assert path("${outputDir}/results_kallisto/multiqc").list().size() == 3}, - // - // Check if files are the same - // - assert snapshot( - workflow, - path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.barcodes.txt" ), - path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.genes.txt" ), - path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.mtx" ), - path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.barcodes.txt" ), - path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.genes.txt" ), - path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.mtx" ), - path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), - path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ) - ).match() + // + // Check if files were produced + // + {assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists()}, + + // + // Check if files are the same + // + {assert snapshot( + workflow, + path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.barcodes.txt" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.genes.txt" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_X.count/counts_unfiltered/cells_x_genes.mtx" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.barcodes.txt" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.genes.txt" ), + path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.mtx" ), + path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), + path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ) + ).match()} + + ) // end of assertAll() } } diff --git a/tests/main_pipeline_star.test b/tests/main_pipeline_star.test index de07c8d3..6616ea87 100644 --- a/tests/main_pipeline_star.test +++ b/tests/main_pipeline_star.test @@ -20,49 +20,53 @@ nextflow_pipeline { then { - // - // General assertions - // + assertAll( - // Did it finish successfully? - assert workflow.success + // + // General assertions + // - // How many tasks were executed? - assert workflow.trace.tasks().size() == 14 + // Did it finish successfully? + {assert workflow.success}, - // How many results were produced? - assert path("${outputDir}/results_star").list().size() == 4 - assert path("${outputDir}/results_star/star").list().size() == 3 - assert path("${outputDir}/results_star/star/mtx_conversions").list().size() == 4 - assert path("${outputDir}/results_star/fastqc").list().size() == 12 - assert path("${outputDir}/results_star/multiqc").list().size() == 3 + // How many tasks were executed? + {assert workflow.trace.tasks().size() == 14}, - // - // Check if files were produced - // - assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists() - assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists() + // How many results were produced? + {assert path("${outputDir}/results_star").list().size() == 4}, + {assert path("${outputDir}/results_star/star").list().size() == 3}, + {assert path("${outputDir}/results_star/star/mtx_conversions").list().size() == 4}, + {assert path("${outputDir}/results_star/fastqc").list().size() == 12}, + {assert path("${outputDir}/results_star/multiqc").list().size() == 3}, - // - // Check if files are the same - // - assert snapshot( - workflow, - path( "${outputDir}/results_star/star/Sample_X/Sample_X.SJ.out.tab" ), - path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Barcodes.stats" ), - path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/matrix.mtx.gz" ), - path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/features.tsv.gz" ), - path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/barcodes.tsv.gz" ), - // path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ), // does not match - path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), - path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.SJ.out.tab" ), - path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Barcodes.stats" ), - path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/matrix.mtx.gz" ), - path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/features.tsv.gz" ), - path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/barcodes.tsv.gz" ), - // path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ), // does not match - path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ), - ).match() + // + // Check if files were produced + // + {assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists()}, + + // + // Check if files are the same + // + {assert snapshot( + workflow, + path( "${outputDir}/results_star/star/Sample_X/Sample_X.SJ.out.tab" ), + path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Barcodes.stats" ), + path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/matrix.mtx.gz" ), + path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/features.tsv.gz" ), + path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/barcodes.tsv.gz" ), + // path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ), // does not match + path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.SJ.out.tab" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Barcodes.stats" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/matrix.mtx.gz" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/features.tsv.gz" ), + path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/barcodes.tsv.gz" ), + // path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ), // does not match + path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ), + ).match()} + + ) // end of assertAll() } } From 0e5e6aabd05a2c0a12601f517f5eddb6ec56680b Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Wed, 14 Feb 2024 08:21:57 +0000 Subject: [PATCH 042/111] update changelog --- CHANGELOG.md | 4 ++++ 1 file changed, 4 insertions(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index a7c8ed27..9f920a4d 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,6 +3,10 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). +## TBD + +- [[#91](https://github.com/nf-core/scrnaseq/issues/91)] - Change from pytests to nf-test + ## v2.5.1 - Fix that cellranger workflow couldn't be run and enable CI for this workflow ([#288](https://github.com/nf-core/scrnaseq/pull/288)). From 6458e2d8aedb6dddfa49accc813c3c76617a6649 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 14 Feb 2024 14:59:57 +0100 Subject: [PATCH 043/111] update shared nf-test config --- tests/nextflow.config | 3 +++ 1 file changed, 3 insertions(+) diff --git a/tests/nextflow.config b/tests/nextflow.config index 66e56d29..7efd4642 100644 --- a/tests/nextflow.config +++ b/tests/nextflow.config @@ -22,6 +22,9 @@ params { gtf = 'https://github.com/nf-core/test-datasets/raw/scrnaseq/reference/gencode.vM19.annotation.chr19.gtf' protocol = '10XV2' + // small dataset does not have sufficient data for emptydrops module + skip_emptydrops = true + validationSchemaIgnoreParams = 'genomes' } From c5cc1ca7571c888692b13cb181e508a789399f7d Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 14 Feb 2024 15:10:34 +0100 Subject: [PATCH 044/111] update alevin file names --- tests/main_pipeline_alevin.test | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/main_pipeline_alevin.test b/tests/main_pipeline_alevin.test index 303a625b..6bdbaba5 100644 --- a/tests/main_pipeline_alevin.test +++ b/tests/main_pipeline_alevin.test @@ -43,8 +43,8 @@ nextflow_pipeline { // // Check if files were produced // - {assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists()}, - {assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_X/Sample_X_raw_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_Y/Sample_Y_raw_matrix.h5ad" ).exists()}, // // Check if files are the same From c5393992afe04cde364c549d300d409aebc8c814 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Wed, 14 Feb 2024 14:57:34 +0000 Subject: [PATCH 045/111] update alevin tests to also include the .rds files --- tests/main_pipeline_alevin.test | 4 +++- tests/main_pipeline_alevin.test.snap | 10 ++++++++-- 2 files changed, 11 insertions(+), 3 deletions(-) diff --git a/tests/main_pipeline_alevin.test b/tests/main_pipeline_alevin.test index 6bdbaba5..32be1eb3 100644 --- a/tests/main_pipeline_alevin.test +++ b/tests/main_pipeline_alevin.test @@ -56,7 +56,9 @@ nextflow_pipeline { path( "${outputDir}/results_alevin/alevin/Sample_X_alevin_results/af_quant/alevin/quants_mat_rows.txt" ), path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_cols.txt" ), path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat.mtx" ), - path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_rows.txt" ) + path( "${outputDir}/results_alevin/alevin/Sample_Y_alevin_results/af_quant/alevin/quants_mat_rows.txt" ), + path( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_X/Sample_X_raw_matrix.rds" ), + path( "${outputDir}/results_alevin/alevin/mtx_conversions/Sample_Y/Sample_Y_raw_matrix.rds" ) ).match()} ) // end of assertAll() diff --git a/tests/main_pipeline_alevin.test.snap b/tests/main_pipeline_alevin.test.snap index 9d194012..b7648793 100644 --- a/tests/main_pipeline_alevin.test.snap +++ b/tests/main_pipeline_alevin.test.snap @@ -25,8 +25,14 @@ "quants_mat_rows.txt:md5,6227df5a13127b71c71fb18cd8574857", "quants_mat_cols.txt:md5,e9868982c17a330392e38c2a5933cf97", "quants_mat.mtx:md5,54cd12666016adce94c025b2e07f4b02", - "quants_mat_rows.txt:md5,6b458a7777260ba90eccbe7919df934b" + "quants_mat_rows.txt:md5,6b458a7777260ba90eccbe7919df934b", + "Sample_X_raw_matrix.rds:md5,ad35ee66bf2fc3d5d4656c19a7e64e2b", + "Sample_Y_raw_matrix.rds:md5,baf584142205b1d42bb6fdab1f22a06a" ], - "timestamp": "2024-01-19T10:28:35.652763852" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T14:49:46.831540515" } } \ No newline at end of file From 2d7c90e7dd8038bb158d7b75620eea3bfc5e0221 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Wed, 14 Feb 2024 15:45:04 +0000 Subject: [PATCH 046/111] update the number of tasks that are executed, and include raw/filtered in the testings (cellranger) --- tests/main_pipeline_cellranger.test | 16 ++++++++++------ tests/main_pipeline_cellranger.test.snap | 16 +++++++++++----- 2 files changed, 21 insertions(+), 11 deletions(-) diff --git a/tests/main_pipeline_cellranger.test b/tests/main_pipeline_cellranger.test index 996c0f76..30723ba3 100644 --- a/tests/main_pipeline_cellranger.test +++ b/tests/main_pipeline_cellranger.test @@ -30,12 +30,12 @@ nextflow_pipeline { {assert workflow.success}, // How many tasks were executed? - {assert workflow.trace.tasks().size() == 15}, + {assert workflow.trace.tasks().size() == 20}, // How many results were produced? {assert path("${outputDir}/results_cellranger").list().size() == 4}, {assert path("${outputDir}/results_cellranger/cellranger").list().size() == 4}, - {assert path("${outputDir}/results_cellranger/cellranger/mtx_conversions").list().size() == 4}, + {assert path("${outputDir}/results_cellranger/cellranger/mtx_conversions").list().size() == 5}, {assert path("${outputDir}/results_cellranger/cellranger/count").list().size() == 3}, {assert path("${outputDir}/results_cellranger/fastqc").list().size() == 12}, {assert path("${outputDir}/results_cellranger/multiqc").list().size() == 3}, @@ -43,8 +43,10 @@ nextflow_pipeline { // // Check if files were produced // - {assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists()}, - {assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_raw_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_raw_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_filtered_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_filtered_matrix.h5ad" ).exists()}, // // Check if files are the same @@ -63,8 +65,10 @@ nextflow_pipeline { path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/barcodes.tsv.gz" ), path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/features.tsv.gz" ), path( "${outputDir}/results_cellranger/cellranger/count/Sample_Y/outs/raw_feature_bc_matrix/matrix.mtx.gz" ), - path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), - path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ) + path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_raw_matrix.rds" ), + path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_raw_matrix.rds" ), + path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_X/Sample_X_filtered_matrix.rds" ), + path( "${outputDir}/results_cellranger/cellranger/mtx_conversions/Sample_Y/Sample_Y_filtered_matrix.rds" ) ).match()} ) // end of assertAll() diff --git a/tests/main_pipeline_cellranger.test.snap b/tests/main_pipeline_cellranger.test.snap index 5f88b954..4aab7554 100644 --- a/tests/main_pipeline_cellranger.test.snap +++ b/tests/main_pipeline_cellranger.test.snap @@ -14,8 +14,8 @@ "errorMessage": "", "trace": { "tasksFailed": 0, - "tasksCount": 15, - "tasksSucceeded": 15 + "tasksCount": 20, + "tasksSucceeded": 20 }, "name": "workflow", "success": true @@ -32,9 +32,15 @@ "barcodes.tsv.gz:md5,081f72b5252ccaf5ffd535ffbd235c4c", "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", "matrix.mtx.gz:md5,a4db04e43e650accc96361a287126a6b", - "Sample_X_matrix.rds:md5,f9191ba575a3ab79ada4807715f18573", - "Sample_Y_matrix.rds:md5,7be3f7b29d668dcf7e951b9f4d371a5e" + "Sample_X_raw_matrix.rds:md5,306a5477ace4d43d851b8389fdfeaf1f", + "Sample_Y_raw_matrix.rds:md5,74b31532da4cae5a8197d690021d77fc", + "Sample_X_filtered_matrix.rds:md5,f9191ba575a3ab79ada4807715f18573", + "Sample_Y_filtered_matrix.rds:md5,7be3f7b29d668dcf7e951b9f4d371a5e" ], - "timestamp": "2024-01-22T15:19:20.134275449" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T15:10:31.638485641" } } \ No newline at end of file From c72c8f203de4d12cd8ec5bfefe20d117d8b66b39 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Wed, 14 Feb 2024 16:23:14 +0000 Subject: [PATCH 047/111] fix naming of generated files (kallisto) --- tests/main_pipeline_kallisto.test | 8 ++++---- tests/main_pipeline_kallisto.test.snap | 10 +++++++--- 2 files changed, 11 insertions(+), 7 deletions(-) diff --git a/tests/main_pipeline_kallisto.test b/tests/main_pipeline_kallisto.test index 98ede8c4..5fa0ead3 100644 --- a/tests/main_pipeline_kallisto.test +++ b/tests/main_pipeline_kallisto.test @@ -44,8 +44,8 @@ nextflow_pipeline { // // Check if files were produced // - {assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists()}, - {assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_raw_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_raw_matrix.h5ad" ).exists()}, // // Check if files are the same @@ -58,8 +58,8 @@ nextflow_pipeline { path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.barcodes.txt" ), path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.genes.txt" ), path( "${outputDir}/results_kallisto/kallisto/Sample_Y.count/counts_unfiltered/cells_x_genes.mtx" ), - path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), - path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ) + path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_X/Sample_X_raw_matrix.rds" ), + path( "${outputDir}/results_kallisto/kallisto/mtx_conversions/Sample_Y/Sample_Y_raw_matrix.rds" ) ).match()} ) // end of assertAll() diff --git a/tests/main_pipeline_kallisto.test.snap b/tests/main_pipeline_kallisto.test.snap index b0fb50bb..eda17cdc 100644 --- a/tests/main_pipeline_kallisto.test.snap +++ b/tests/main_pipeline_kallisto.test.snap @@ -26,9 +26,13 @@ "cells_x_genes.barcodes.txt:md5,488437e1f5477243697efb93366e5676", "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", "cells_x_genes.mtx:md5,af90e05b404490f6cb133ab7f62949f8", - "Sample_X_matrix.rds:md5,f0e43f69403f4b2e7704065421592ad0", - "Sample_Y_matrix.rds:md5,61809156e64dbdaf254cbc1c3456588e" + "Sample_X_raw_matrix.rds:md5,f0e43f69403f4b2e7704065421592ad0", + "Sample_Y_raw_matrix.rds:md5,61809156e64dbdaf254cbc1c3456588e" ], - "timestamp": "2024-01-23T12:19:47.921508953" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T16:04:36.951736171" } } \ No newline at end of file From 5d1d78336316ffa8a30eddd7bbb256d02b99c93d Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Wed, 14 Feb 2024 16:38:36 +0000 Subject: [PATCH 048/111] update the amount of tasks and generated file names raw/filtered (star) --- tests/main_pipeline_star.test | 18 ++++++++++-------- tests/main_pipeline_star.test.snap | 16 +++++++++++----- 2 files changed, 21 insertions(+), 13 deletions(-) diff --git a/tests/main_pipeline_star.test b/tests/main_pipeline_star.test index 6616ea87..f7afdfc9 100644 --- a/tests/main_pipeline_star.test +++ b/tests/main_pipeline_star.test @@ -30,20 +30,22 @@ nextflow_pipeline { {assert workflow.success}, // How many tasks were executed? - {assert workflow.trace.tasks().size() == 14}, + {assert workflow.trace.tasks().size() == 19}, // How many results were produced? {assert path("${outputDir}/results_star").list().size() == 4}, {assert path("${outputDir}/results_star/star").list().size() == 3}, - {assert path("${outputDir}/results_star/star/mtx_conversions").list().size() == 4}, + {assert path("${outputDir}/results_star/star/mtx_conversions").list().size() == 5}, {assert path("${outputDir}/results_star/fastqc").list().size() == 12}, {assert path("${outputDir}/results_star/multiqc").list().size() == 3}, // // Check if files were produced // - {assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ).exists()}, - {assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_raw_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_raw_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_filtered_matrix.h5ad" ).exists()}, + {assert new File( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_filtered_matrix.h5ad" ).exists()}, // // Check if files are the same @@ -55,15 +57,15 @@ nextflow_pipeline { path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/matrix.mtx.gz" ), path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/features.tsv.gz" ), path( "${outputDir}/results_star/star/Sample_X/Sample_X.Solo.out/Gene/filtered/barcodes.tsv.gz" ), - // path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.h5ad" ), // does not match - path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_matrix.rds" ), path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.SJ.out.tab" ), path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Barcodes.stats" ), path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/matrix.mtx.gz" ), path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/features.tsv.gz" ), path( "${outputDir}/results_star/star/Sample_Y/Sample_Y.Solo.out/Gene/filtered/barcodes.tsv.gz" ), - // path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.h5ad" ), // does not match - path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_matrix.rds" ), + path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_raw_matrix.rds" ), + path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_raw_matrix.rds" ), + path( "${outputDir}/results_star/star/mtx_conversions/Sample_X/Sample_X_filtered_matrix.rds" ), + path( "${outputDir}/results_star/star/mtx_conversions/Sample_Y/Sample_Y_filtered_matrix.rds" ), ).match()} ) // end of assertAll() diff --git a/tests/main_pipeline_star.test.snap b/tests/main_pipeline_star.test.snap index ef086f60..0d45f3b4 100644 --- a/tests/main_pipeline_star.test.snap +++ b/tests/main_pipeline_star.test.snap @@ -14,8 +14,8 @@ "errorMessage": "", "trace": { "tasksFailed": 0, - "tasksCount": 14, - "tasksSucceeded": 14 + "tasksCount": 19, + "tasksSucceeded": 19 }, "name": "workflow", "success": true @@ -25,14 +25,20 @@ "matrix.mtx.gz:md5,6a923393343aa1a69b0cf1bd998c9285", "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", "barcodes.tsv.gz:md5,9a7dacaa1779ea43c1507a947fe6992a", - "Sample_X_matrix.rds:md5,aa2d36dd8507aba864347c88e4ce0d27", "Sample_Y.SJ.out.tab:md5,98bd31104a860cf80119dc30d938d163", "Barcodes.stats:md5,2dbf1ae426c1afd97903ee001f0db5ce", "matrix.mtx.gz:md5,0ae080bd0002e350531a5816e159345e", "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", "barcodes.tsv.gz:md5,9b695b0b91bcb146ec9c4688ca10a690", - "Sample_Y_matrix.rds:md5,d459af8f99258bcc88b80b2f7c58e911" + "Sample_X_raw_matrix.rds:md5,31604db3e7846acc8d9a60b1a171ce78", + "Sample_Y_raw_matrix.rds:md5,1a52c823e91acce2b29621c8c99c8c72", + "Sample_X_filtered_matrix.rds:md5,aa2d36dd8507aba864347c88e4ce0d27", + "Sample_Y_filtered_matrix.rds:md5,d459af8f99258bcc88b80b2f7c58e911" ], - "timestamp": "2024-01-19T15:46:22.470527538" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T16:30:25.7971791" } } \ No newline at end of file From 4095c3d68cc393c5a043efe798b130d7331750ad Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Wed, 14 Feb 2024 16:39:04 +0000 Subject: [PATCH 049/111] update gitignore --- .gitignore | 1 + 1 file changed, 1 insertion(+) diff --git a/.gitignore b/.gitignore index a9ba2c52..9dfcadb3 100644 --- a/.gitignore +++ b/.gitignore @@ -10,3 +10,4 @@ log/ reports/ testme.sh .nf-test/ +.nf-test.log From 6981c44acae7f71950cf5f76b8de6c84603572e8 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Thu, 15 Feb 2024 07:27:56 +0000 Subject: [PATCH 050/111] add new params to schema --- nextflow_schema.json | 9 +++++++++ 1 file changed, 9 insertions(+) diff --git a/nextflow_schema.json b/nextflow_schema.json index a53cc55e..f9d2fdd0 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -84,6 +84,10 @@ "skip_fastqc": { "type": "boolean", "description": "Skip FastQC" + }, + "skip_emptydrops": { + "type": "boolean", + "description": "Skip custom empty drops filter module" } } }, @@ -219,6 +223,11 @@ "description": "Type of workflow. Use `lamanno` for RNA velocity based on La Manno et al. 2018 logic. Use `nucleus` for RNA velocity on single-nucleus RNA-seq reads. Use `kite` for feature barcoding. Use `kite: 10xFB` for 10x Genomics Feature Barcoding technology. (default: standard)", "fa_icon": "fas fa-fish", "enum": ["standard", "lamanno", "nucleus", "kite", "kite: 10xFB"] + }, + "kb_filter": { + "type": "boolean", + "fa_icon": "fas fa-fish", + "description": "Activate Kallisto/BUStools filtering algorithm" } } }, From bee4f67a2609fdf952c86b1af3ca95f6b7fc03f7 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Tue, 20 Feb 2024 15:30:41 +0000 Subject: [PATCH 051/111] Template update for nf-core/tools version 2.13 --- .editorconfig | 9 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/branch.yml | 2 +- .github/workflows/ci.yml | 7 +- .github/workflows/clean-up.yml | 2 +- .github/workflows/download_pipeline.yml | 17 +- .github/workflows/linting.yml | 12 +- .github/workflows/linting_comment.yml | 4 +- .github/workflows/release-announcements.yml | 11 +- README.md | 5 +- assets/multiqc_config.yml | 2 + assets/schema_input.json | 21 +- bin/check_samplesheet.py | 259 ----------- conf/modules.config | 8 - lib/NfcoreTemplate.groovy | 356 -------------- lib/Utils.groovy | 47 -- lib/WorkflowMain.groovy | 77 --- lib/WorkflowScrnaseq.groovy | 122 ----- main.nf | 100 ++-- modules.json | 28 +- modules/local/samplesheet_check.nf | 31 -- .../dumpsoftwareversions/environment.yml | 7 - .../custom/dumpsoftwareversions/main.nf | 24 - .../custom/dumpsoftwareversions/meta.yml | 37 -- .../templates/dumpsoftwareversions.py | 102 ---- .../dumpsoftwareversions/tests/main.nf.test | 43 -- .../tests/main.nf.test.snap | 33 -- .../dumpsoftwareversions/tests/tags.yml | 2 - modules/nf-core/fastqc/tests/main.nf.test | 14 +- .../nf-core/fastqc/tests/main.nf.test.snap | 76 ++- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- modules/nf-core/multiqc/tests/main.nf.test | 13 +- .../nf-core/multiqc/tests/main.nf.test.snap | 32 +- nextflow.config | 5 +- nextflow_schema.json | 1 + pyproject.toml | 8 +- subworkflows/local/input_check.nf | 44 -- .../utils_nfcore_scrnaseq_pipeline/main.nf | 247 ++++++++++ .../nf-core/utils_nextflow_pipeline/main.nf | 126 +++++ .../nf-core/utils_nextflow_pipeline/meta.yml | 38 ++ .../tests/main.function.nf.test | 54 +++ .../tests/main.function.nf.test.snap | 12 + .../tests/main.workflow.nf.test | 123 +++++ .../tests/nextflow.config | 9 + .../utils_nextflow_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfcore_pipeline/main.nf | 440 ++++++++++++++++++ .../nf-core/utils_nfcore_pipeline/meta.yml | 24 + .../tests/main.function.nf.test | 134 ++++++ .../tests/main.function.nf.test.snap | 138 ++++++ .../tests/main.workflow.nf.test | 29 ++ .../tests/main.workflow.nf.test.snap | 15 + .../tests/nextflow.config | 9 + .../utils_nfcore_pipeline/tests/tags.yml | 2 + .../nf-core/utils_nfvalidation_plugin/main.nf | 62 +++ .../utils_nfvalidation_plugin/meta.yml | 44 ++ .../tests/main.nf.test | 200 ++++++++ .../tests/nextflow_schema.json | 96 ++++ .../utils_nfvalidation_plugin/tests/tags.yml | 2 + workflows/scrnaseq.nf | 133 ++---- 61 files changed, 2108 insertions(+), 1406 deletions(-) delete mode 100755 bin/check_samplesheet.py delete mode 100755 lib/NfcoreTemplate.groovy delete mode 100644 lib/Utils.groovy delete mode 100755 lib/WorkflowMain.groovy delete mode 100755 lib/WorkflowScrnaseq.groovy delete mode 100644 modules/local/samplesheet_check.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/environment.yml delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/main.nf delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/meta.yml delete mode 100755 modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap delete mode 100644 modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml delete mode 100644 subworkflows/local/input_check.nf create mode 100644 subworkflows/local/utils_nfcore_scrnaseq_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/main.nf create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/meta.yml create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config create mode 100644 subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/main.nf create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json create mode 100644 subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml diff --git a/.editorconfig b/.editorconfig index 9b990088..dd9ffa53 100644 --- a/.editorconfig +++ b/.editorconfig @@ -18,7 +18,12 @@ end_of_line = unset insert_final_newline = unset trim_trailing_whitespace = unset indent_style = unset -indent_size = unset +[/subworkflows/nf-core/**] +charset = unset +end_of_line = unset +insert_final_newline = unset +trim_trailing_whitespace = unset +indent_style = unset [/assets/email*] indent_size = unset @@ -28,5 +33,5 @@ indent_size = unset indent_style = unset # ignore python -[*.{py}] +[*.{py,md}] indent_style = unset diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index 33ee6733..fd263945 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index edc7b3a3..1f58192f 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@v2 + uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@v4 + - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/branch.yml b/.github/workflows/branch.yml index 68083c82..b7f206b8 100644 --- a/.github/workflows/branch.yml +++ b/.github/workflows/branch.yml @@ -19,7 +19,7 @@ jobs: # NOTE - this doesn't currently work if the PR is coming from a fork, due to limitations in GitHub actions secrets - name: Post PR comment if: failure() - uses: mshick/add-pr-comment@v2 + uses: mshick/add-pr-comment@b8f338c590a895d50bcbfa6c5859251edc8952fc # v2 with: message: | ## This PR is against the `master` branch :x: diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index de0c4594..e2c989ca 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -28,13 +28,16 @@ jobs: - "latest-everything" steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 with: version: "${{ matrix.NXF_VER }}" + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Run pipeline with test data # TODO nf-core: You can customise CI pipeline run tests as required # For example: adding multiple test runs with different parameters diff --git a/.github/workflows/clean-up.yml b/.github/workflows/clean-up.yml index e37cfda5..0b6b1f27 100644 --- a/.github/workflows/clean-up.yml +++ b/.github/workflows/clean-up.yml @@ -10,7 +10,7 @@ jobs: issues: write pull-requests: write steps: - - uses: actions/stale@v9 + - uses: actions/stale@28ca1036281a5e5922ead5184a1bbf96e5fc984e # v9 with: stale-issue-message: "This issue has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment otherwise this issue will be closed in 20 days." stale-pr-message: "This PR has been tagged as awaiting-changes or awaiting-feedback by an nf-core contributor. Remove stale label or add a comment if it is still useful." diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 8611458a..f823210d 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -6,6 +6,11 @@ name: Test successful pipeline download with 'nf-core download' # - the head branch of the pull request is updated, i.e. if fixes for a release are pushed last minute to dev. on: workflow_dispatch: + inputs: + testbranch: + description: "The specific branch you wish to utilize for the test execution of nf-core download." + required: true + default: "dev" pull_request: types: - opened @@ -23,13 +28,13 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" - - uses: eWaterCycle/setup-singularity@v7 + - uses: eWaterCycle/setup-singularity@931d4e31109e875b13309ae1d07c70ca8fbc8537 # v7 with: singularity-version: 3.8.3 @@ -42,13 +47,13 @@ jobs: run: | echo "REPO_LOWERCASE=${GITHUB_REPOSITORY,,}" >> ${GITHUB_ENV} echo "REPOTITLE_LOWERCASE=$(basename ${GITHUB_REPOSITORY,,})" >> ${GITHUB_ENV} - echo "REPO_BRANCH=${GITHUB_REF#refs/heads/}" >> ${GITHUB_ENV} + echo "REPO_BRANCH=${{ github.event.inputs.testbranch || 'dev' }}" >> ${GITHUB_ENV} - name: Download the pipeline env: NXF_SINGULARITY_CACHEDIR: ./ run: | - nf-core download ${{ env.REPO_LOWERCASE }} \ + nf-core download ${{ env.REPO_LOWERCASE }} \ --revision ${{ env.REPO_BRANCH }} \ --outdir ./${{ env.REPOTITLE_LOWERCASE }} \ --compress "none" \ @@ -64,4 +69,4 @@ jobs: env: NXF_SINGULARITY_CACHEDIR: ./ NXF_SINGULARITY_HOME_MOUNT: true - run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results + run: nextflow run ./${{ env.REPOTITLE_LOWERCASE }}/$( sed 's/\W/_/g' <<< ${{ env.REPO_BRANCH }}) -stub -profile test,singularity --outdir ./results diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 81cd098e..748b4311 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -14,10 +14,10 @@ jobs: pre-commit: runs-on: ubuntu-latest steps: - - uses: actions/checkout@v4 + - uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Set up Python 3.11 - uses: actions/setup-python@v5 + uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: 3.11 cache: "pip" @@ -32,12 +32,12 @@ jobs: runs-on: ubuntu-latest steps: - name: Check out pipeline code - uses: actions/checkout@v4 + uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@v1 + uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.11" architecture: "x64" @@ -60,7 +60,7 @@ jobs: - name: Upload linting log file artifact if: ${{ always() }} - uses: actions/upload-artifact@v4 + uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 with: name: linting-logs path: | diff --git a/.github/workflows/linting_comment.yml b/.github/workflows/linting_comment.yml index 147bcd10..b706875f 100644 --- a/.github/workflows/linting_comment.yml +++ b/.github/workflows/linting_comment.yml @@ -11,7 +11,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Download lint results - uses: dawidd6/action-download-artifact@v3 + uses: dawidd6/action-download-artifact@f6b0bace624032e30a85a8fd9c1a7f8f611f5737 # v3 with: workflow: linting.yml workflow_conclusion: completed @@ -21,7 +21,7 @@ jobs: run: echo "pr_number=$(cat linting-logs/PR_number.txt)" >> $GITHUB_OUTPUT - name: Post PR comment - uses: marocchino/sticky-pull-request-comment@v2 + uses: marocchino/sticky-pull-request-comment@331f8f5b4215f0445d3c07b4967662a32a2d3e31 # v2 with: GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }} number: ${{ steps.pr_number.outputs.pr_number }} diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index 21ac3f06..c3674af2 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -9,6 +9,11 @@ jobs: toot: runs-on: ubuntu-latest steps: + - name: get topics and convert to hashtags + id: get_topics + run: | + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + - uses: rzr/fediverse-action@master with: access-token: ${{ secrets.MASTODON_ACCESS_TOKEN }} @@ -20,11 +25,13 @@ jobs: Please see the changelog: ${{ github.event.release.html_url }} + ${{ steps.get_topics.outputs.GITHUB_OUTPUT }} #nfcore #openscience #nextflow #bioinformatics + send-tweet: runs-on: ubuntu-latest steps: - - uses: actions/setup-python@v5 + - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: python-version: "3.10" - name: Install dependencies @@ -56,7 +63,7 @@ jobs: bsky-post: runs-on: ubuntu-latest steps: - - uses: zentered/bluesky-post-action@v0.1.0 + - uses: zentered/bluesky-post-action@80dbe0a7697de18c15ad22f4619919ceb5ccf597 # v0.1.0 with: post: | Pipeline release! ${{ github.repository }} v${{ github.event.release.tag_name }} - ${{ github.event.release.name }}! diff --git a/README.md b/README.md index f997b3d7..c80f96b1 100644 --- a/README.md +++ b/README.md @@ -4,8 +4,9 @@ nf-core/scrnaseq -[![GitHub Actions CI Status](https://github.com/nf-core/scrnaseq/workflows/nf-core%20CI/badge.svg)](https://github.com/nf-core/scrnaseq/actions?query=workflow%3A%22nf-core+CI%22) -[![GitHub Actions Linting Status](https://github.com/nf-core/scrnaseq/workflows/nf-core%20linting/badge.svg)](https://github.com/nf-core/scrnaseq/actions?query=workflow%3A%22nf-core+linting%22)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/scrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) + +[![GitHub Actions CI Status](https://github.com/nf-core/scrnaseq/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/scrnaseq/actions/workflows/ci.yml) +[![GitHub Actions Linting Status](https://github.com/nf-core/scrnaseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/scrnaseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/scrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 051ed54e..584bae69 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -11,3 +11,5 @@ report_section_order: order: -1002 export_plots: true + +disable_version_detection: true diff --git a/assets/schema_input.json b/assets/schema_input.json index 0a93254d..dab0450a 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -10,25 +10,22 @@ "sample": { "type": "string", "pattern": "^\\S+$", - "errorMessage": "Sample name must be provided and cannot contain spaces" + "errorMessage": "Sample name must be provided and cannot contain spaces", + "meta": ["id"] }, "fastq_1": { "type": "string", + "format": "file-path", + "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 1 must be provided, cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" }, "fastq_2": { - "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'", - "anyOf": [ - { - "type": "string", - "pattern": "^\\S+\\.f(ast)?q\\.gz$" - }, - { - "type": "string", - "maxLength": 0 - } - ] + "type": "string", + "format": "file-path", + "exists": true, + "pattern": "^\\S+\\.f(ast)?q\\.gz$", + "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" } }, "required": ["sample", "fastq_1"] diff --git a/bin/check_samplesheet.py b/bin/check_samplesheet.py deleted file mode 100755 index 4a758fe0..00000000 --- a/bin/check_samplesheet.py +++ /dev/null @@ -1,259 +0,0 @@ -#!/usr/bin/env python - - -"""Provide a command line tool to validate and transform tabular samplesheets.""" - - -import argparse -import csv -import logging -import sys -from collections import Counter -from pathlib import Path - -logger = logging.getLogger() - - -class RowChecker: - """ - Define a service that can validate and transform each given row. - - Attributes: - modified (list): A list of dicts, where each dict corresponds to a previously - validated and transformed row. The order of rows is maintained. - - """ - - VALID_FORMATS = ( - ".fq.gz", - ".fastq.gz", - ) - - def __init__( - self, - sample_col="sample", - first_col="fastq_1", - second_col="fastq_2", - single_col="single_end", - **kwargs, - ): - """ - Initialize the row checker with the expected column names. - - Args: - sample_col (str): The name of the column that contains the sample name - (default "sample"). - first_col (str): The name of the column that contains the first (or only) - FASTQ file path (default "fastq_1"). - second_col (str): The name of the column that contains the second (if any) - FASTQ file path (default "fastq_2"). - single_col (str): The name of the new column that will be inserted and - records whether the sample contains single- or paired-end sequencing - reads (default "single_end"). - - """ - super().__init__(**kwargs) - self._sample_col = sample_col - self._first_col = first_col - self._second_col = second_col - self._single_col = single_col - self._seen = set() - self.modified = [] - - def validate_and_transform(self, row): - """ - Perform all validations on the given row and insert the read pairing status. - - Args: - row (dict): A mapping from column headers (keys) to elements of that row - (values). - - """ - self._validate_sample(row) - self._validate_first(row) - self._validate_second(row) - self._validate_pair(row) - self._seen.add((row[self._sample_col], row[self._first_col])) - self.modified.append(row) - - def _validate_sample(self, row): - """Assert that the sample name exists and convert spaces to underscores.""" - if len(row[self._sample_col]) <= 0: - raise AssertionError("Sample input is required.") - # Sanitize samples slightly. - row[self._sample_col] = row[self._sample_col].replace(" ", "_") - - def _validate_first(self, row): - """Assert that the first FASTQ entry is non-empty and has the right format.""" - if len(row[self._first_col]) <= 0: - raise AssertionError("At least the first FASTQ file is required.") - self._validate_fastq_format(row[self._first_col]) - - def _validate_second(self, row): - """Assert that the second FASTQ entry has the right format if it exists.""" - if len(row[self._second_col]) > 0: - self._validate_fastq_format(row[self._second_col]) - - def _validate_pair(self, row): - """Assert that read pairs have the same file extension. Report pair status.""" - if row[self._first_col] and row[self._second_col]: - row[self._single_col] = False - first_col_suffix = Path(row[self._first_col]).suffixes[-2:] - second_col_suffix = Path(row[self._second_col]).suffixes[-2:] - if first_col_suffix != second_col_suffix: - raise AssertionError("FASTQ pairs must have the same file extensions.") - else: - row[self._single_col] = True - - def _validate_fastq_format(self, filename): - """Assert that a given filename has one of the expected FASTQ extensions.""" - if not any(filename.endswith(extension) for extension in self.VALID_FORMATS): - raise AssertionError( - f"The FASTQ file has an unrecognized extension: {filename}\n" - f"It should be one of: {', '.join(self.VALID_FORMATS)}" - ) - - def validate_unique_samples(self): - """ - Assert that the combination of sample name and FASTQ filename is unique. - - In addition to the validation, also rename all samples to have a suffix of _T{n}, where n is the - number of times the same sample exist, but with different FASTQ files, e.g., multiple runs per experiment. - - """ - if len(self._seen) != len(self.modified): - raise AssertionError("The pair of sample name and FASTQ must be unique.") - seen = Counter() - for row in self.modified: - sample = row[self._sample_col] - seen[sample] += 1 - row[self._sample_col] = f"{sample}_T{seen[sample]}" - - -def read_head(handle, num_lines=10): - """Read the specified number of lines from the current position in the file.""" - lines = [] - for idx, line in enumerate(handle): - if idx == num_lines: - break - lines.append(line) - return "".join(lines) - - -def sniff_format(handle): - """ - Detect the tabular format. - - Args: - handle (text file): A handle to a `text file`_ object. The read position is - expected to be at the beginning (index 0). - - Returns: - csv.Dialect: The detected tabular format. - - .. _text file: - https://docs.python.org/3/glossary.html#term-text-file - - """ - peek = read_head(handle) - handle.seek(0) - sniffer = csv.Sniffer() - dialect = sniffer.sniff(peek) - return dialect - - -def check_samplesheet(file_in, file_out): - """ - Check that the tabular samplesheet has the structure expected by nf-core pipelines. - - Validate the general shape of the table, expected columns, and each row. Also add - an additional column which records whether one or two FASTQ reads were found. - - Args: - file_in (pathlib.Path): The given tabular samplesheet. The format can be either - CSV, TSV, or any other format automatically recognized by ``csv.Sniffer``. - file_out (pathlib.Path): Where the validated and transformed samplesheet should - be created; always in CSV format. - - Example: - This function checks that the samplesheet follows the following structure, - see also the `viral recon samplesheet`_:: - - sample,fastq_1,fastq_2 - SAMPLE_PE,SAMPLE_PE_RUN1_1.fastq.gz,SAMPLE_PE_RUN1_2.fastq.gz - SAMPLE_PE,SAMPLE_PE_RUN2_1.fastq.gz,SAMPLE_PE_RUN2_2.fastq.gz - SAMPLE_SE,SAMPLE_SE_RUN1_1.fastq.gz, - - .. _viral recon samplesheet: - https://raw.githubusercontent.com/nf-core/test-datasets/viralrecon/samplesheet/samplesheet_test_illumina_amplicon.csv - - """ - required_columns = {"sample", "fastq_1", "fastq_2"} - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_in.open(newline="") as in_handle: - reader = csv.DictReader(in_handle, dialect=sniff_format(in_handle)) - # Validate the existence of the expected header columns. - if not required_columns.issubset(reader.fieldnames): - req_cols = ", ".join(required_columns) - logger.critical(f"The sample sheet **must** contain these column headers: {req_cols}.") - sys.exit(1) - # Validate each row. - checker = RowChecker() - for i, row in enumerate(reader): - try: - checker.validate_and_transform(row) - except AssertionError as error: - logger.critical(f"{str(error)} On line {i + 2}.") - sys.exit(1) - checker.validate_unique_samples() - header = list(reader.fieldnames) - header.insert(1, "single_end") - # See https://docs.python.org/3.9/library/csv.html#id3 to read up on `newline=""`. - with file_out.open(mode="w", newline="") as out_handle: - writer = csv.DictWriter(out_handle, header, delimiter=",") - writer.writeheader() - for row in checker.modified: - writer.writerow(row) - - -def parse_args(argv=None): - """Define and immediately parse command line arguments.""" - parser = argparse.ArgumentParser( - description="Validate and transform a tabular samplesheet.", - epilog="Example: python check_samplesheet.py samplesheet.csv samplesheet.valid.csv", - ) - parser.add_argument( - "file_in", - metavar="FILE_IN", - type=Path, - help="Tabular input samplesheet in CSV or TSV format.", - ) - parser.add_argument( - "file_out", - metavar="FILE_OUT", - type=Path, - help="Transformed output samplesheet in CSV format.", - ) - parser.add_argument( - "-l", - "--log-level", - help="The desired log level (default WARNING).", - choices=("CRITICAL", "ERROR", "WARNING", "INFO", "DEBUG"), - default="WARNING", - ) - return parser.parse_args(argv) - - -def main(argv=None): - """Coordinate argument parsing and program execution.""" - args = parse_args(argv) - logging.basicConfig(level=args.log_level, format="[%(levelname)s] %(message)s") - if not args.file_in.is_file(): - logger.error(f"The given input file {args.file_in} was not found!") - sys.exit(2) - args.file_out.parent.mkdir(parents=True, exist_ok=True) - check_samplesheet(args.file_in, args.file_out) - - -if __name__ == "__main__": - sys.exit(main()) diff --git a/conf/modules.config b/conf/modules.config index d91c6aba..e3ea8fa6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -18,14 +18,6 @@ process { saveAs: { filename -> filename.equals('versions.yml') ? null : filename } ] - withName: SAMPLESHEET_CHECK { - publishDir = [ - path: { "${params.outdir}/pipeline_info" }, - mode: params.publish_dir_mode, - saveAs: { filename -> filename.equals('versions.yml') ? null : filename } - ] - } - withName: FASTQC { ext.args = '--quiet' } diff --git a/lib/NfcoreTemplate.groovy b/lib/NfcoreTemplate.groovy deleted file mode 100755 index e248e4c3..00000000 --- a/lib/NfcoreTemplate.groovy +++ /dev/null @@ -1,356 +0,0 @@ -// -// This file holds several functions used within the nf-core pipeline template. -// - -import org.yaml.snakeyaml.Yaml -import groovy.json.JsonOutput -import nextflow.extension.FilesEx - -class NfcoreTemplate { - - // - // Check AWS Batch related parameters have been specified correctly - // - public static void awsBatch(workflow, params) { - if (workflow.profile.contains('awsbatch')) { - // Check params.awsqueue and params.awsregion have been set if running on AWSBatch - assert (params.awsqueue && params.awsregion) : "Specify correct --awsqueue and --awsregion parameters on AWSBatch!" - // Check outdir paths to be S3 buckets if running on AWSBatch - assert params.outdir.startsWith('s3:') : "Outdir not on S3 - specify S3 Bucket to run on AWSBatch!" - } - } - - // - // Warn if a -profile or Nextflow config has not been provided to run the pipeline - // - public static void checkConfigProvided(workflow, log) { - if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { - log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + - "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + - " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + - " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + - " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + - "Please refer to the quick start section and usage docs for the pipeline.\n " - } - } - - // - // Generate version string - // - public static String version(workflow) { - String version_string = "" - - if (workflow.manifest.version) { - def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' - version_string += "${prefix_v}${workflow.manifest.version}" - } - - if (workflow.commitId) { - def git_shortsha = workflow.commitId.substring(0, 7) - version_string += "-g${git_shortsha}" - } - - return version_string - } - - // - // Construct and send completion email - // - public static void email(workflow, params, summary_params, projectDir, log, multiqc_report=[]) { - - // Set up the e-mail variables - def subject = "[$workflow.manifest.name] Successful: $workflow.runName" - if (!workflow.success) { - subject = "[$workflow.manifest.name] FAILED: $workflow.runName" - } - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['Date Started'] = workflow.start - misc_fields['Date Completed'] = workflow.complete - misc_fields['Pipeline script file path'] = workflow.scriptFile - misc_fields['Pipeline script hash ID'] = workflow.scriptId - if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository - if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId - if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision - misc_fields['Nextflow Version'] = workflow.nextflow.version - misc_fields['Nextflow Build'] = workflow.nextflow.build - misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp - - def email_fields = [:] - email_fields['version'] = NfcoreTemplate.version(workflow) - email_fields['runName'] = workflow.runName - email_fields['success'] = workflow.success - email_fields['dateComplete'] = workflow.complete - email_fields['duration'] = workflow.duration - email_fields['exitStatus'] = workflow.exitStatus - email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - email_fields['errorReport'] = (workflow.errorReport ?: 'None') - email_fields['commandLine'] = workflow.commandLine - email_fields['projectDir'] = workflow.projectDir - email_fields['summary'] = summary << misc_fields - - // On success try attach the multiqc report - def mqc_report = null - try { - if (workflow.success) { - mqc_report = multiqc_report.getVal() - if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { - if (mqc_report.size() > 1) { - log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" - } - mqc_report = mqc_report[0] - } - } - } catch (all) { - if (multiqc_report) { - log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" - } - } - - // Check if we are only sending emails on failure - def email_address = params.email - if (!params.email && params.email_on_fail && !workflow.success) { - email_address = params.email_on_fail - } - - // Render the TXT template - def engine = new groovy.text.GStringTemplateEngine() - def tf = new File("$projectDir/assets/email_template.txt") - def txt_template = engine.createTemplate(tf).make(email_fields) - def email_txt = txt_template.toString() - - // Render the HTML template - def hf = new File("$projectDir/assets/email_template.html") - def html_template = engine.createTemplate(hf).make(email_fields) - def email_html = html_template.toString() - - // Render the sendmail template - def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit - def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "$projectDir", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] - def sf = new File("$projectDir/assets/sendmail_template.txt") - def sendmail_template = engine.createTemplate(sf).make(smail_fields) - def sendmail_html = sendmail_template.toString() - - // Send the HTML e-mail - Map colors = logColours(params.monochrome_logs) - if (email_address) { - try { - if (params.plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } - // Try to send HTML e-mail using sendmail - def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") - sendmail_tf.withWriter { w -> w << sendmail_html } - [ 'sendmail', '-t' ].execute() << sendmail_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" - } catch (all) { - // Catch failures and try with plaintext - def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] - if ( mqc_report != null && mqc_report.size() <= max_multiqc_email_size.toBytes() ) { - mail_cmd += [ '-A', mqc_report ] - } - mail_cmd.execute() << email_html - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" - } - } - - // Write summary e-mail HTML to a file - def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") - output_hf.withWriter { w -> w << email_html } - FilesEx.copyTo(output_hf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.html"); - output_hf.delete() - - // Write summary e-mail TXT to a file - def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") - output_tf.withWriter { w -> w << email_txt } - FilesEx.copyTo(output_tf.toPath(), "${params.outdir}/pipeline_info/pipeline_report.txt"); - output_tf.delete() - } - - // - // Construct and send a notification to a web server as JSON - // e.g. Microsoft Teams and Slack - // - public static void IM_notification(workflow, params, summary_params, projectDir, log) { - def hook_url = params.hook_url - - def summary = [:] - for (group in summary_params.keySet()) { - summary << summary_params[group] - } - - def misc_fields = [:] - misc_fields['start'] = workflow.start - misc_fields['complete'] = workflow.complete - misc_fields['scriptfile'] = workflow.scriptFile - misc_fields['scriptid'] = workflow.scriptId - if (workflow.repository) misc_fields['repository'] = workflow.repository - if (workflow.commitId) misc_fields['commitid'] = workflow.commitId - if (workflow.revision) misc_fields['revision'] = workflow.revision - misc_fields['nxf_version'] = workflow.nextflow.version - misc_fields['nxf_build'] = workflow.nextflow.build - misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp - - def msg_fields = [:] - msg_fields['version'] = NfcoreTemplate.version(workflow) - msg_fields['runName'] = workflow.runName - msg_fields['success'] = workflow.success - msg_fields['dateComplete'] = workflow.complete - msg_fields['duration'] = workflow.duration - msg_fields['exitStatus'] = workflow.exitStatus - msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') - msg_fields['errorReport'] = (workflow.errorReport ?: 'None') - msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") - msg_fields['projectDir'] = workflow.projectDir - msg_fields['summary'] = summary << misc_fields - - // Render the JSON template - def engine = new groovy.text.GStringTemplateEngine() - // Different JSON depending on the service provider - // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format - def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" - def hf = new File("$projectDir/assets/${json_path}") - def json_template = engine.createTemplate(hf).make(msg_fields) - def json_message = json_template.toString() - - // POST - def post = new URL(hook_url).openConnection(); - post.setRequestMethod("POST") - post.setDoOutput(true) - post.setRequestProperty("Content-Type", "application/json") - post.getOutputStream().write(json_message.getBytes("UTF-8")); - def postRC = post.getResponseCode(); - if (! postRC.equals(200)) { - log.warn(post.getErrorStream().getText()); - } - } - - // - // Dump pipeline parameters in a json file - // - public static void dump_parameters(workflow, params) { - def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') - def filename = "params_${timestamp}.json" - def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") - def jsonStr = JsonOutput.toJson(params) - temp_pf.text = JsonOutput.prettyPrint(jsonStr) - - FilesEx.copyTo(temp_pf.toPath(), "${params.outdir}/pipeline_info/params_${timestamp}.json") - temp_pf.delete() - } - - // - // Print pipeline summary on completion - // - public static void summary(workflow, params, log) { - Map colors = logColours(params.monochrome_logs) - if (workflow.success) { - if (workflow.stats.ignoredCount == 0) { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" - } - } else { - log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" - } - } - - // - // ANSII Colours used for terminal logging - // - public static Map logColours(Boolean monochrome_logs) { - Map colorcodes = [:] - - // Reset / Meta - colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" - colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" - colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" - colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" - colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" - colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" - colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" - - // Regular Colors - colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" - colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" - colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" - colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" - colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" - colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" - colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" - colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" - - // Bold - colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" - colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" - colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" - colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" - colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" - colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" - colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" - colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" - - // Underline - colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" - colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" - colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" - colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" - colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" - colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" - colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" - colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" - - // High Intensity - colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" - colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" - colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" - colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" - colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" - colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" - colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" - colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" - - // Bold High Intensity - colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" - colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" - colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" - colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" - colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" - colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" - colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" - colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" - - return colorcodes - } - - // - // Does what is says on the tin - // - public static String dashedLine(monochrome_logs) { - Map colors = logColours(monochrome_logs) - return "-${colors.dim}----------------------------------------------------${colors.reset}-" - } - - // - // nf-core logo - // - public static String logo(workflow, monochrome_logs) { - Map colors = logColours(monochrome_logs) - String workflow_version = NfcoreTemplate.version(workflow) - String.format( - """\n - ${dashedLine(monochrome_logs)} - ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} - ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} - ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} - ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} - ${colors.green}`._,._,\'${colors.reset} - ${colors.purple} ${workflow.manifest.name} ${workflow_version}${colors.reset} - ${dashedLine(monochrome_logs)} - """.stripIndent() - ) - } -} diff --git a/lib/Utils.groovy b/lib/Utils.groovy deleted file mode 100644 index 8d030f4e..00000000 --- a/lib/Utils.groovy +++ /dev/null @@ -1,47 +0,0 @@ -// -// This file holds several Groovy functions that could be useful for any Nextflow pipeline -// - -import org.yaml.snakeyaml.Yaml - -class Utils { - - // - // When running with -profile conda, warn if channels have not been set-up appropriately - // - public static void checkCondaChannels(log) { - Yaml parser = new Yaml() - def channels = [] - try { - def config = parser.load("conda config --show channels".execute().text) - channels = config.channels - } catch(NullPointerException | IOException e) { - log.warn "Could not verify conda channel configuration." - return - } - - // Check that all channels are present - // This channel list is ordered by required channel priority. - def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] - def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean - - // Check that they are in the right order - def channel_priority_violation = false - def n = required_channels_in_order.size() - for (int i = 0; i < n - 1; i++) { - channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) - } - - if (channels_missing | channel_priority_violation) { - log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " There is a problem with your Conda configuration!\n\n" + - " You will need to set-up the conda-forge and bioconda channels correctly.\n" + - " Please refer to https://bioconda.github.io/\n" + - " The observed channel order is \n" + - " ${channels}\n" + - " but the following channel order is required:\n" + - " ${required_channels_in_order}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - } - } -} diff --git a/lib/WorkflowMain.groovy b/lib/WorkflowMain.groovy deleted file mode 100755 index e4d0a286..00000000 --- a/lib/WorkflowMain.groovy +++ /dev/null @@ -1,77 +0,0 @@ -// -// This file holds several functions specific to the main.nf workflow in the nf-core/scrnaseq pipeline -// - -import nextflow.Nextflow - -class WorkflowMain { - - // - // Citation string for pipeline - // - public static String citation(workflow) { - return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + - // TODO nf-core: Add Zenodo DOI for pipeline after first release - //"* The pipeline\n" + - //" https://doi.org/10.5281/zenodo.XXXXXXX\n\n" + - "* The nf-core framework\n" + - " https://doi.org/10.1038/s41587-020-0439-x\n\n" + - "* Software dependencies\n" + - " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" - } - - - // - // Validate parameters and print summary to screen - // - public static void initialise(workflow, params, log, args) { - - // Print workflow version and exit on --version - if (params.version) { - String workflow_version = NfcoreTemplate.version(workflow) - log.info "${workflow.manifest.name} ${workflow_version}" - System.exit(0) - } - - // Check that a -profile or Nextflow config has been provided to run the pipeline - NfcoreTemplate.checkConfigProvided(workflow, log) - // Check that the profile doesn't contain spaces and doesn't end with a trailing comma - checkProfile(workflow.profile, args, log) - - // Check that conda channels are set-up correctly - if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { - Utils.checkCondaChannels(log) - } - - // Check AWS batch settings - NfcoreTemplate.awsBatch(workflow, params) - - // Check input has been provided - if (!params.input) { - Nextflow.error("Please provide an input samplesheet to the pipeline e.g. '--input samplesheet.csv'") - } - } - // - // Get attribute from genome config file e.g. fasta - // - public static Object getGenomeAttribute(params, attribute) { - if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { - if (params.genomes[ params.genome ].containsKey(attribute)) { - return params.genomes[ params.genome ][ attribute ] - } - } - return null - } - - // - // Exit pipeline if --profile contains spaces - // - private static void checkProfile(profile, args, log) { - if (profile.endsWith(',')) { - Nextflow.error "Profile cannot end with a trailing comma. Please remove the comma from the end of the profile string.\nHint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - if (args[0]) { - log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${args[0]}` has been detected.\n Hint: A common mistake is to provide multiple values to `-profile` separated by spaces. Please use commas to separate profiles instead,e.g., `-profile docker,test`." - } - } -} diff --git a/lib/WorkflowScrnaseq.groovy b/lib/WorkflowScrnaseq.groovy deleted file mode 100755 index 04860c20..00000000 --- a/lib/WorkflowScrnaseq.groovy +++ /dev/null @@ -1,122 +0,0 @@ -// -// This file holds several functions specific to the workflow/scrnaseq.nf in the nf-core/scrnaseq pipeline -// - -import nextflow.Nextflow -import groovy.text.SimpleTemplateEngine - -class WorkflowScrnaseq { - - // - // Check and validate parameters - // - public static void initialise(params, log) { - - genomeExistsError(params, log) - - - if (!params.fasta) { - Nextflow.error "Genome fasta file not specified with e.g. '--fasta genome.fa' or via a detectable config file." - } - } - - // - // Get workflow summary for MultiQC - // - public static String paramsSummaryMultiqc(workflow, summary) { - String summary_section = '' - for (group in summary.keySet()) { - def group_params = summary.get(group) // This gets the parameters of that particular group - if (group_params) { - summary_section += "

$group

\n" - summary_section += "
\n" - for (param in group_params.keySet()) { - summary_section += "
$param
${group_params.get(param) ?: 'N/A'}
\n" - } - summary_section += "
\n" - } - } - - String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" - yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" - yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" - yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" - yaml_file_text += "plot_type: 'html'\n" - yaml_file_text += "data: |\n" - yaml_file_text += "${summary_section}" - return yaml_file_text - } - - // - // Generate methods description for MultiQC - // - - public static String toolCitationText(params) { - - // TODO nf-core: Optionally add in-text citation tools to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def citation_text = [ - "Tools used in the workflow included:", - "FastQC (Andrews 2010),", - "MultiQC (Ewels et al. 2016)", - "." - ].join(' ').trim() - - return citation_text - } - - public static String toolBibliographyText(params) { - - // TODO Optionally add bibliographic entries to this list. - // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", - // Uncomment function in methodsDescriptionText to render in MultiQC report - def reference_text = [ - "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", - "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " - ].join(' ').trim() - - return reference_text - } - - public static String methodsDescriptionText(run_workflow, mqc_methods_yaml, params) { - // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file - def meta = [:] - meta.workflow = run_workflow.toMap() - meta["manifest_map"] = run_workflow.manifest.toMap() - - // Pipeline DOI - meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" - meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " - - // Tool references - meta["tool_citations"] = "" - meta["tool_bibliography"] = "" - - // TODO Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! - //meta["tool_citations"] = toolCitationText(params).replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") - //meta["tool_bibliography"] = toolBibliographyText(params) - - - def methods_text = mqc_methods_yaml.text - - def engine = new SimpleTemplateEngine() - def description_html = engine.createTemplate(methods_text).make(meta) - - return description_html - } - - // - // Exit pipeline if incorrect --genome key provided - // - private static void genomeExistsError(params, log) { - if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { - def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + - " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + - " Currently, the available genome keys are:\n" + - " ${params.genomes.keySet().join(", ")}\n" + - "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" - Nextflow.error(error_string) - } - } -} diff --git a/main.nf b/main.nf index dd6b9fbc..fe1b3bad 100644 --- a/main.nf +++ b/main.nf @@ -13,66 +13,96 @@ nextflow.enable.dsl = 2 /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - GENOME PARAMETER VALUES + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS / WORKFLOWS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// TODO nf-core: Remove this line if you don't need a FASTA file -// This is an example of how to use getGenomeAttribute() to fetch parameters -// from igenomes.config using `--genome` -params.fasta = WorkflowMain.getGenomeAttribute(params, 'fasta') +include { SCRNASEQ } from './workflows/scrnaseq' +include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_scrnaseq_pipeline' +include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_scrnaseq_pipeline' + +include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_scrnaseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - VALIDATE & PRINT PARAMETER SUMMARY + GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { validateParameters; paramsHelp } from 'plugin/nf-validation' - -// Print help message if needed -if (params.help) { - def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) - def citation = '\n' + WorkflowMain.citation(workflow) + '\n' - def String command = "nextflow run ${workflow.manifest.name} --input samplesheet.csv --genome GRCh37 -profile docker" - log.info logo + paramsHelp(command) + citation + NfcoreTemplate.dashedLine(params.monochrome_logs) - System.exit(0) -} - -// Validate input parameters -if (params.validate_params) { - validateParameters() -} - -WorkflowMain.initialise(workflow, params, log, args) +// TODO nf-core: Remove this line if you don't need a FASTA file +// This is an example of how to use getGenomeAttribute() to fetch parameters +// from igenomes.config using `--genome` +params.fasta = getGenomeAttribute('fasta') /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - NAMED WORKFLOW FOR PIPELINE + NAMED WORKFLOWS FOR PIPELINE ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { SCRNASEQ } from './workflows/scrnaseq' - // -// WORKFLOW: Run main nf-core/scrnaseq analysis pipeline +// WORKFLOW: Run main analysis pipeline depending on type of input // workflow NFCORE_SCRNASEQ { - SCRNASEQ () -} + take: + samplesheet // channel: samplesheet read in from --input + + main: + + // + // WORKFLOW: Run pipeline + // + SCRNASEQ ( + samplesheet + ) + + emit: + multiqc_report = SCRNASEQ.out.multiqc_report // channel: /path/to/multiqc_report.html + +} /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN ALL WORKFLOWS + RUN MAIN WORKFLOW ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// -// WORKFLOW: Execute a single named workflow for the pipeline -// See: https://github.com/nf-core/rnaseq/issues/619 -// workflow { - NFCORE_SCRNASEQ () + + main: + + // + // SUBWORKFLOW: Run initialisation tasks + // + PIPELINE_INITIALISATION ( + params.version, + params.help, + params.validate_params, + params.monochrome_logs, + args, + params.outdir, + params.input + ) + + // + // WORKFLOW: Run main workflow + // + NFCORE_SCRNASEQ ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_SCRNASEQ.out.multiqc_report + ) } /* diff --git a/modules.json b/modules.json index 364c63d0..59464672 100644 --- a/modules.json +++ b/modules.json @@ -5,22 +5,36 @@ "https://github.com/nf-core/modules.git": { "modules": { "nf-core": { - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", - "git_sha": "c9488585ce7bd35ccd2a30faa2371454c8112fb9", + "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", "installed_by": ["modules"] }, "multiqc": { "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", + "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", "installed_by": ["modules"] } } + }, + "subworkflows": { + "nf-core": { + "utils_nextflow_pipeline": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + }, + "utils_nfcore_pipeline": { + "branch": "master", + "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "installed_by": ["subworkflows"] + }, + "utils_nfvalidation_plugin": { + "branch": "master", + "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "installed_by": ["subworkflows"] + } + } } } } diff --git a/modules/local/samplesheet_check.nf b/modules/local/samplesheet_check.nf deleted file mode 100644 index d3934fdd..00000000 --- a/modules/local/samplesheet_check.nf +++ /dev/null @@ -1,31 +0,0 @@ -process SAMPLESHEET_CHECK { - tag "$samplesheet" - label 'process_single' - - conda "conda-forge::python=3.8.3" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/python:3.8.3' : - 'biocontainers/python:3.8.3' }" - - input: - path samplesheet - - output: - path '*.csv' , emit: csv - path "versions.yml", emit: versions - - when: - task.ext.when == null || task.ext.when - - script: // This script is bundled with the pipeline, in nf-core/scrnaseq/bin/ - """ - check_samplesheet.py \\ - $samplesheet \\ - samplesheet.valid.csv - - cat <<-END_VERSIONS > versions.yml - "${task.process}": - python: \$(python --version | sed 's/Python //g') - END_VERSIONS - """ -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/environment.yml b/modules/nf-core/custom/dumpsoftwareversions/environment.yml deleted file mode 100644 index 9b3272bc..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/environment.yml +++ /dev/null @@ -1,7 +0,0 @@ -name: custom_dumpsoftwareversions -channels: - - conda-forge - - bioconda - - defaults -dependencies: - - bioconda::multiqc=1.19 diff --git a/modules/nf-core/custom/dumpsoftwareversions/main.nf b/modules/nf-core/custom/dumpsoftwareversions/main.nf deleted file mode 100644 index f2187611..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/main.nf +++ /dev/null @@ -1,24 +0,0 @@ -process CUSTOM_DUMPSOFTWAREVERSIONS { - label 'process_single' - - // Requires `pyyaml` which does not have a dedicated container but is in the MultiQC container - conda "${moduleDir}/environment.yml" - container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" - - input: - path versions - - output: - path "software_versions.yml" , emit: yml - path "software_versions_mqc.yml", emit: mqc_yml - path "versions.yml" , emit: versions - - when: - task.ext.when == null || task.ext.when - - script: - def args = task.ext.args ?: '' - template 'dumpsoftwareversions.py' -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/meta.yml b/modules/nf-core/custom/dumpsoftwareversions/meta.yml deleted file mode 100644 index 5f15a5fd..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/meta.yml +++ /dev/null @@ -1,37 +0,0 @@ -# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/modules/meta-schema.json -name: custom_dumpsoftwareversions -description: Custom module used to dump software versions within the nf-core pipeline template -keywords: - - custom - - dump - - version -tools: - - custom: - description: Custom module used to dump software versions within the nf-core pipeline template - homepage: https://github.com/nf-core/tools - documentation: https://github.com/nf-core/tools - licence: ["MIT"] -input: - - versions: - type: file - description: YML file containing software versions - pattern: "*.yml" -output: - - yml: - type: file - description: Standard YML file containing software versions - pattern: "software_versions.yml" - - mqc_yml: - type: file - description: MultiQC custom content YML file containing software versions - pattern: "software_versions_mqc.yml" - - versions: - type: file - description: File containing software versions - pattern: "versions.yml" -authors: - - "@drpatelh" - - "@grst" -maintainers: - - "@drpatelh" - - "@grst" diff --git a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py b/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py deleted file mode 100755 index e55b8d43..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/templates/dumpsoftwareversions.py +++ /dev/null @@ -1,102 +0,0 @@ -#!/usr/bin/env python - - -"""Provide functions to merge multiple versions.yml files.""" - - -import platform -from textwrap import dedent - -import yaml - - -def _make_versions_html(versions): - """Generate a tabular HTML output of all versions for MultiQC.""" - html = [ - dedent( - """\\ - -
    Process Name \\", + " \\ Software Version
    CUSTOM_DUMPSOFTWAREVERSIONSpython3.11.7
    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
    File typeConventional base calls
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    - - - - - - - - """ - ) - ] - for process, tmp_versions in sorted(versions.items()): - html.append("") - for i, (tool, version) in enumerate(sorted(tmp_versions.items())): - html.append( - dedent( - f"""\\ - - - - - - """ - ) - ) - html.append("") - html.append("
    Process Name Software Version
    {process if (i == 0) else ''}{tool}{version}
    ") - return "\\n".join(html) - - -def main(): - """Load all version files and generate merged output.""" - versions_this_module = {} - versions_this_module["${task.process}"] = { - "python": platform.python_version(), - "yaml": yaml.__version__, - } - - with open("$versions") as f: - versions_by_process = yaml.load(f, Loader=yaml.BaseLoader) | versions_this_module - - # aggregate versions by the module name (derived from fully-qualified process name) - versions_by_module = {} - for process, process_versions in versions_by_process.items(): - module = process.split(":")[-1] - try: - if versions_by_module[module] != process_versions: - raise AssertionError( - "We assume that software versions are the same between all modules. " - "If you see this error-message it means you discovered an edge-case " - "and should open an issue in nf-core/tools. " - ) - except KeyError: - versions_by_module[module] = process_versions - - versions_by_module["Workflow"] = { - "Nextflow": "$workflow.nextflow.version", - "$workflow.manifest.name": "$workflow.manifest.version", - } - - versions_mqc = { - "id": "software_versions", - "section_name": "${workflow.manifest.name} Software Versions", - "section_href": "https://github.com/${workflow.manifest.name}", - "plot_type": "html", - "description": "are collected at run time from the software output.", - "data": _make_versions_html(versions_by_module), - } - - with open("software_versions.yml", "w") as f: - yaml.dump(versions_by_module, f, default_flow_style=False) - with open("software_versions_mqc.yml", "w") as f: - yaml.dump(versions_mqc, f, default_flow_style=False) - - with open("versions.yml", "w") as f: - yaml.dump(versions_this_module, f, default_flow_style=False) - - -if __name__ == "__main__": - main() diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test deleted file mode 100644 index b1e1630b..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test +++ /dev/null @@ -1,43 +0,0 @@ -nextflow_process { - - name "Test Process CUSTOM_DUMPSOFTWAREVERSIONS" - script "../main.nf" - process "CUSTOM_DUMPSOFTWAREVERSIONS" - tag "modules" - tag "modules_nfcore" - tag "custom" - tag "dumpsoftwareversions" - tag "custom/dumpsoftwareversions" - - test("Should run without failures") { - when { - process { - """ - def tool1_version = ''' - TOOL1: - tool1: 0.11.9 - '''.stripIndent() - - def tool2_version = ''' - TOOL2: - tool2: 1.9 - '''.stripIndent() - - input[0] = Channel.of(tool1_version, tool2_version).collectFile() - """ - } - } - - then { - assertAll( - { assert process.success }, - { assert snapshot( - process.out.versions, - file(process.out.mqc_yml[0]).readLines()[0..10], - file(process.out.yml[0]).readLines()[0..7] - ).match() - } - ) - } - } -} diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap b/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap deleted file mode 100644 index 5f59a936..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/main.nf.test.snap +++ /dev/null @@ -1,33 +0,0 @@ -{ - "Should run without failures": { - "content": [ - [ - "versions.yml:md5,76d454d92244589d32455833f7c1ba6d" - ], - [ - "data: \"\\n\\n \\n \\n \\n \\n \\n \\n \\n\\", - " \\n\\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n \\n \\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\", - " \\ \\n\\n\\n\\n\\n\\n \\n\\", - " \\ \\n \\n\\n\\n\\n\\", - " \\n\\n \\n \\n\\" - ], - [ - "CUSTOM_DUMPSOFTWAREVERSIONS:", - " python: 3.11.7", - " yaml: 5.4.1", - "TOOL1:", - " tool1: 0.11.9", - "TOOL2:", - " tool2: '1.9'", - "Workflow:" - ] - ], - "timestamp": "2024-01-09T23:01:18.710682" - } -} \ No newline at end of file diff --git a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml b/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml deleted file mode 100644 index 405aa24a..00000000 --- a/modules/nf-core/custom/dumpsoftwareversions/tests/tags.yml +++ /dev/null @@ -1,2 +0,0 @@ -custom/dumpsoftwareversions: - - modules/nf-core/custom/dumpsoftwareversions/** diff --git a/modules/nf-core/fastqc/tests/main.nf.test b/modules/nf-core/fastqc/tests/main.nf.test index 1f21c664..70edae4d 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test +++ b/modules/nf-core/fastqc/tests/main.nf.test @@ -33,7 +33,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_single") } ) } } @@ -63,7 +63,7 @@ nextflow_process { { assert path(process.out.html[0][1][0]).text.contains("") }, { assert path(process.out.html[0][1][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_paired") } ) } } @@ -89,7 +89,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_interleaved") } ) } } @@ -115,7 +115,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/test_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_bam") } ) } } @@ -153,7 +153,7 @@ nextflow_process { { assert path(process.out.html[0][1][2]).text.contains("") }, { assert path(process.out.html[0][1][3]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_multiple") } ) } } @@ -179,7 +179,7 @@ nextflow_process { { assert process.out.zip[0][1] ==~ ".*/mysample_fastqc.zip" }, { assert path(process.out.html[0][1]).text.contains("") }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("fastqc_versions_custom_prefix") } ) } } @@ -204,7 +204,7 @@ nextflow_process { { assert process.success }, { assert snapshot(process.out.html.collect { file(it[1]).getName() } + process.out.zip.collect { file(it[1]).getName() } + - process.out.versions ).match() } + process.out.versions ).match("fastqc_stub") } ) } } diff --git a/modules/nf-core/fastqc/tests/main.nf.test.snap b/modules/nf-core/fastqc/tests/main.nf.test.snap index 5d624bb8..86f7c311 100644 --- a/modules/nf-core/fastqc/tests/main.nf.test.snap +++ b/modules/nf-core/fastqc/tests/main.nf.test.snap @@ -1,5 +1,17 @@ { - "sarscov2 single-end [fastq] - stub": { + "fastqc_versions_interleaved": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:07.293713" + }, + "fastqc_stub": { "content": [ [ "test.html", @@ -7,14 +19,70 @@ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:40:57.254299" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:31:01.425198" + }, + "fastqc_versions_multiple": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:55.797907" + }, + "fastqc_versions_bam": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:40:26.795862" + }, + "fastqc_versions_single": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:27.043675" + }, + "fastqc_versions_paired": { + "content": [ + [ + "versions.yml:md5,e1cc25ca8af856014824abd842e93978" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:39:47.584191" }, - "versions": { + "fastqc_versions_custom_prefix": { "content": [ [ "versions.yml:md5,e1cc25ca8af856014824abd842e93978" ] ], - "timestamp": "2024-01-17T18:36:50.033627" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-01-31T17:41:14.576531" } } \ No newline at end of file diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 7625b752..2212096a 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.19 + - bioconda::multiqc=1.20 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 1b9f7c43..354f4430 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.19--pyhdfd78af_0' : - 'biocontainers/multiqc:1.19--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : + 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test b/modules/nf-core/multiqc/tests/main.nf.test index d0438eda..f1c4242e 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test +++ b/modules/nf-core/multiqc/tests/main.nf.test @@ -3,6 +3,7 @@ nextflow_process { name "Test Process MULTIQC" script "../main.nf" process "MULTIQC" + tag "modules" tag "modules_nfcore" tag "multiqc" @@ -12,7 +13,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -25,7 +26,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_single") } ) } @@ -36,7 +37,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = Channel.of(file("https://github.com/nf-core/tools/raw/dev/nf_core/pipeline-template/assets/multiqc_config.yml", checkIfExists: true)) input[2] = [] input[3] = [] @@ -49,7 +50,7 @@ nextflow_process { { assert process.success }, { assert process.out.report[0] ==~ ".*/multiqc_report.html" }, { assert process.out.data[0] ==~ ".*/multiqc_data" }, - { assert snapshot(process.out.versions).match("versions") } + { assert snapshot(process.out.versions).match("multiqc_versions_config") } ) } } @@ -61,7 +62,7 @@ nextflow_process { when { process { """ - input[0] = Channel.of([file(params.test_data['sarscov2']['illumina']['test_1_fastq_gz_fastqc_zip'], checkIfExists: true)]) + input[0] = Channel.of(file(params.modules_testdata_base_path + 'genomics/sarscov2/illumina/fastqc/test_fastqc.zip', checkIfExists: true)) input[1] = [] input[2] = [] input[3] = [] @@ -75,7 +76,7 @@ nextflow_process { { assert snapshot(process.out.report.collect { file(it).getName() } + process.out.data.collect { file(it).getName() } + process.out.plots.collect { file(it).getName() } + - process.out.versions ).match() } + process.out.versions ).match("multiqc_stub") } ) } diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index d37e7304..c204b488 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -1,21 +1,41 @@ { - "versions": { + "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:02:49.911994" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:28:51.744211298" }, - "sarscov2 single-end [fastqc] - stub": { + "multiqc_stub": { "content": [ [ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" ] ], - "timestamp": "2024-01-09T23:03:14.524346" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:28.847433492" + }, + "multiqc_versions_config": { + "content": [ + [ + "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-14T09:29:13.223621555" } } \ No newline at end of file diff --git a/nextflow.config b/nextflow.config index 5ece77c6..9508cefa 100644 --- a/nextflow.config +++ b/nextflow.config @@ -16,9 +16,7 @@ params { genome = null igenomes_base = 's3://ngi-igenomes/igenomes/' igenomes_ignore = false - - - // MultiQC options + fasta = null// MultiQC options multiqc_config = null multiqc_title = null multiqc_logo = null @@ -43,7 +41,6 @@ params { custom_config_base = "https://raw.githubusercontent.com/nf-core/configs/${params.custom_config_version}" config_profile_contact = null config_profile_url = null - // Max resource options // Defaults only, expecting to be overwritten diff --git a/nextflow_schema.json b/nextflow_schema.json index 37c2128c..233b47d4 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -16,6 +16,7 @@ "type": "string", "format": "file-path", "exists": true, + "schema": "assets/schema_input.json", "mimetype": "text/csv", "pattern": "^\\S+\\.csv$", "description": "Path to comma-separated file containing information about the samples in the experiment.", diff --git a/pyproject.toml b/pyproject.toml index 7d08e1c8..56110621 100644 --- a/pyproject.toml +++ b/pyproject.toml @@ -3,11 +3,13 @@ [tool.ruff] line-length = 120 target-version = "py38" -select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] cache-dir = "~/.cache/ruff" -[tool.ruff.isort] +[tool.ruff.lint] +select = ["I", "E1", "E4", "E7", "E9", "F", "UP", "N"] + +[tool.ruff.lint.isort] known-first-party = ["nf_core"] -[tool.ruff.per-file-ignores] +[tool.ruff.lint.per-file-ignores] "__init__.py" = ["E402", "F401"] diff --git a/subworkflows/local/input_check.nf b/subworkflows/local/input_check.nf deleted file mode 100644 index 0aecf87f..00000000 --- a/subworkflows/local/input_check.nf +++ /dev/null @@ -1,44 +0,0 @@ -// -// Check input samplesheet and get read channels -// - -include { SAMPLESHEET_CHECK } from '../../modules/local/samplesheet_check' - -workflow INPUT_CHECK { - take: - samplesheet // file: /path/to/samplesheet.csv - - main: - SAMPLESHEET_CHECK ( samplesheet ) - .csv - .splitCsv ( header:true, sep:',' ) - .map { create_fastq_channel(it) } - .set { reads } - - emit: - reads // channel: [ val(meta), [ reads ] ] - versions = SAMPLESHEET_CHECK.out.versions // channel: [ versions.yml ] -} - -// Function to get list of [ meta, [ fastq_1, fastq_2 ] ] -def create_fastq_channel(LinkedHashMap row) { - // create meta map - def meta = [:] - meta.id = row.sample - meta.single_end = row.single_end.toBoolean() - - // add path(s) of the fastq file(s) to the meta map - def fastq_meta = [] - if (!file(row.fastq_1).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 1 FastQ file does not exist!\n${row.fastq_1}" - } - if (meta.single_end) { - fastq_meta = [ meta, [ file(row.fastq_1) ] ] - } else { - if (!file(row.fastq_2).exists()) { - exit 1, "ERROR: Please check input samplesheet -> Read 2 FastQ file does not exist!\n${row.fastq_2}" - } - fastq_meta = [ meta, [ file(row.fastq_1), file(row.fastq_2) ] ] - } - return fastq_meta -} diff --git a/subworkflows/local/utils_nfcore_scrnaseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_scrnaseq_pipeline/main.nf new file mode 100644 index 00000000..dc90ad90 --- /dev/null +++ b/subworkflows/local/utils_nfcore_scrnaseq_pipeline/main.nf @@ -0,0 +1,247 @@ +// +// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// + +/* +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ + IMPORT FUNCTIONS / MODULES / SUBWORKFLOWS +~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ +*/ + +include { UTILS_NFVALIDATION_PLUGIN } from '../../nf-core/utils_nfvalidation_plugin' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { fromSamplesheet } from 'plugin/nf-validation' +include { UTILS_NEXTFLOW_PIPELINE } from '../../nf-core/utils_nextflow_pipeline' +include { completionEmail } from '../../nf-core/utils_nfcore_pipeline' +include { completionSummary } from '../../nf-core/utils_nfcore_pipeline' +include { dashedLine } from '../../nf-core/utils_nfcore_pipeline' +include { nfCoreLogo } from '../../nf-core/utils_nfcore_pipeline' +include { imNotification } from '../../nf-core/utils_nfcore_pipeline' +include { UTILS_NFCORE_PIPELINE } from '../../nf-core/utils_nfcore_pipeline' +include { workflowCitation } from '../../nf-core/utils_nfcore_pipeline' + +/* +======================================================================================== + SUBWORKFLOW TO INITIALISE PIPELINE +======================================================================================== +*/ + +workflow PIPELINE_INITIALISATION { + + take: + version // boolean: Display version and exit + help // boolean: Display help text + validate_params // boolean: Boolean whether to validate parameters against the schema at runtime + monochrome_logs // boolean: Do not use coloured log outputs + nextflow_cli_args // array: List of positional nextflow CLI args + outdir // string: The output directory where the results will be saved + input // string: Path to input samplesheet + + main: + + ch_versions = Channel.empty() + + // + // Print version and exit if required and dump pipeline parameters to JSON file + // + UTILS_NEXTFLOW_PIPELINE ( + version, + true, + outdir, + workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1 + ) + + // + // Validate parameters and generate parameter summary to stdout + // + pre_help_text = nfCoreLogo(monochrome_logs) + post_help_text = '\n' + workflowCitation() + '\n' + dashedLine(monochrome_logs) + def String workflow_command = "nextflow run ${workflow.manifest.name} -profile --input samplesheet.csv --outdir " + UTILS_NFVALIDATION_PLUGIN ( + help, + workflow_command, + pre_help_text, + post_help_text, + validate_params, + "nextflow_schema.json" + ) + + // + // Check config provided to the pipeline + // + UTILS_NFCORE_PIPELINE ( + nextflow_cli_args + ) + // + // Custom validation for pipeline parameters + // + validateInputParameters() + + // + // Create channel from input file provided through params.input + // + Channel + .fromSamplesheet("input") + .map { + meta, fastq_1, fastq_2 -> + if (!fastq_2) { + return [ meta.id, meta + [ single_end:true ], [ fastq_1 ] ] + } else { + return [ meta.id, meta + [ single_end:false ], [ fastq_1, fastq_2 ] ] + } + } + .groupTuple() + .map { + validateInputSamplesheet(it) + } + .map { + meta, fastqs -> + return [ meta, fastqs.flatten() ] + } + .set { ch_samplesheet } + + emit: + samplesheet = ch_samplesheet + versions = ch_versions +} + +/* +======================================================================================== + SUBWORKFLOW FOR PIPELINE COMPLETION +======================================================================================== +*/ + +workflow PIPELINE_COMPLETION { + + take: + email // string: email address + email_on_fail // string: email address sent on pipeline failure + plaintext_email // boolean: Send plain-text email instead of HTML + outdir // path: Path to output directory where results will be published + monochrome_logs // boolean: Disable ANSI colour codes in log output + hook_url // string: hook URL for notifications + multiqc_report // string: Path to MultiQC report + + main: + + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + + // + // Completion email and summary + // + workflow.onComplete { + if (email || email_on_fail) { + completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs, multiqc_report.toList()) + } + + completionSummary(monochrome_logs) + + if (hook_url) { + imNotification(summary_params, hook_url) + } + } +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ +// +// Check and validate pipeline parameters +// +def validateInputParameters() { + genomeExistsError() +}// +// Validate channels from input samplesheet +// +def validateInputSamplesheet(input) { + def (metas, fastqs) = input[1..2] + + // Check that multiple runs of the same sample are of the same datatype i.e. single-end / paired-end + def endedness_ok = metas.collect{ it.single_end }.unique().size == 1 + if (!endedness_ok) { + error("Please check input samplesheet -> Multiple runs of a sample must be of the same datatype i.e. single-end or paired-end: ${metas[0].id}") + } + + return [ metas[0], fastqs ] +} +// +// Get attribute from genome config file e.g. fasta +// +def getGenomeAttribute(attribute) { + if (params.genomes && params.genome && params.genomes.containsKey(params.genome)) { + if (params.genomes[ params.genome ].containsKey(attribute)) { + return params.genomes[ params.genome ][ attribute ] + } + } + return null +} + +// +// Exit pipeline if incorrect --genome key provided +// +def genomeExistsError() { + if (params.genomes && params.genome && !params.genomes.containsKey(params.genome)) { + def error_string = "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " Genome '${params.genome}' not found in any config files provided to the pipeline.\n" + + " Currently, the available genome keys are:\n" + + " ${params.genomes.keySet().join(", ")}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + error(error_string) + } +}// +// Generate methods description for MultiQC +// +def toolCitationText() { + // TODO nf-core: Optionally add in-text citation tools to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "Tool (Foo et al. 2023)" : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def citation_text = [ + "Tools used in the workflow included:", + "FastQC (Andrews 2010),", + "MultiQC (Ewels et al. 2016)", + "." + ].join(' ').trim() + + return citation_text +} + +def toolBibliographyText() { + // TODO nf-core: Optionally add bibliographic entries to this list. + // Can use ternary operators to dynamically construct based conditions, e.g. params["run_xyz"] ? "
  • Author (2023) Pub name, Journal, DOI
    • " : "", + // Uncomment function in methodsDescriptionText to render in MultiQC report + def reference_text = [ + "
  • Andrews S, (2010) FastQC, URL: https://www.bioinformatics.babraham.ac.uk/projects/fastqc/).
    • ", + "
  • Ewels, P., Magnusson, M., Lundin, S., & Käller, M. (2016). MultiQC: summarize analysis results for multiple tools and samples in a single report. Bioinformatics , 32(19), 3047–3048. doi: /10.1093/bioinformatics/btw354
    • " + ].join(' ').trim() + + return reference_text +} + +def methodsDescriptionText(mqc_methods_yaml) { + // Convert to a named map so can be used as with familar NXF ${workflow} variable syntax in the MultiQC YML file + def meta = [:] + meta.workflow = workflow.toMap() + meta["manifest_map"] = workflow.manifest.toMap() + + // Pipeline DOI + meta["doi_text"] = meta.manifest_map.doi ? "(doi: ${meta.manifest_map.doi})" : "" + meta["nodoi_text"] = meta.manifest_map.doi ? "": "
  • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
    • " + + // Tool references + meta["tool_citations"] = "" + meta["tool_bibliography"] = "" + + // TODO nf-core: Only uncomment below if logic in toolCitationText/toolBibliographyText has been filled! + // meta["tool_citations"] = toolCitationText().replaceAll(", \\.", ".").replaceAll("\\. \\.", ".").replaceAll(", \\.", ".") + // meta["tool_bibliography"] = toolBibliographyText() + + + def methods_text = mqc_methods_yaml.text + + def engine = new groovy.text.SimpleTemplateEngine() + def description_html = engine.createTemplate(methods_text).make(meta) + + return description_html.toString() +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/main.nf b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf new file mode 100644 index 00000000..ac31f28f --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/main.nf @@ -0,0 +1,126 @@ +// +// Subworkflow with functionality that may be useful for any Nextflow pipeline +// + +import org.yaml.snakeyaml.Yaml +import groovy.json.JsonOutput +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NEXTFLOW_PIPELINE { + + take: + print_version // boolean: print version + dump_parameters // boolean: dump parameters + outdir // path: base directory used to publish pipeline results + check_conda_channels // boolean: check conda channels + + main: + + // + // Print workflow version and exit on --version + // + if (print_version) { + log.info "${workflow.manifest.name} ${getWorkflowVersion()}" + System.exit(0) + } + + // + // Dump pipeline parameters to a JSON file + // + if (dump_parameters && outdir) { + dumpParametersToJSON(outdir) + } + + // + // When running with Conda, warn if channels have not been set-up appropriately + // + if (check_conda_channels) { + checkCondaChannels() + } + + emit: + dummy_emit = true +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Generate version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Dump pipeline parameters to a JSON file +// +def dumpParametersToJSON(outdir) { + def timestamp = new java.util.Date().format( 'yyyy-MM-dd_HH-mm-ss') + def filename = "params_${timestamp}.json" + def temp_pf = new File(workflow.launchDir.toString(), ".${filename}") + def jsonStr = JsonOutput.toJson(params) + temp_pf.text = JsonOutput.prettyPrint(jsonStr) + + FilesEx.copyTo(temp_pf.toPath(), "${outdir}/pipeline_info/params_${timestamp}.json") + temp_pf.delete() +} + +// +// When running with -profile conda, warn if channels have not been set-up appropriately +// +def checkCondaChannels() { + Yaml parser = new Yaml() + def channels = [] + try { + def config = parser.load("conda config --show channels".execute().text) + channels = config.channels + } catch(NullPointerException | IOException e) { + log.warn "Could not verify conda channel configuration." + return + } + + // Check that all channels are present + // This channel list is ordered by required channel priority. + def required_channels_in_order = ['conda-forge', 'bioconda', 'defaults'] + def channels_missing = ((required_channels_in_order as Set) - (channels as Set)) as Boolean + + // Check that they are in the right order + def channel_priority_violation = false + def n = required_channels_in_order.size() + for (int i = 0; i < n - 1; i++) { + channel_priority_violation |= !(channels.indexOf(required_channels_in_order[i]) < channels.indexOf(required_channels_in_order[i+1])) + } + + if (channels_missing | channel_priority_violation) { + log.warn "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~\n" + + " There is a problem with your Conda configuration!\n\n" + + " You will need to set-up the conda-forge and bioconda channels correctly.\n" + + " Please refer to https://bioconda.github.io/\n" + + " The observed channel order is \n" + + " ${channels}\n" + + " but the following channel order is required:\n" + + " ${required_channels_in_order}\n" + + "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml new file mode 100644 index 00000000..e5c3a0a8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/meta.yml @@ -0,0 +1,38 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NEXTFLOW_PIPELINE" +description: Subworkflow with functionality that may be useful for any Nextflow pipeline +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - print_version: + type: boolean + description: | + Print the version of the pipeline and exit + - dump_parameters: + type: boolean + description: | + Dump the parameters of the pipeline to a JSON file + - output_directory: + type: directory + description: Path to output dir to write JSON file to. + pattern: "results/" + - check_conda_channel: + type: boolean + description: | + Check if the conda channel priority is correct. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" + - "@drpatelh" +maintainers: + - "@adamrtalbot" + - "@drpatelh" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..8ed4310c --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -0,0 +1,54 @@ + +nextflow_function { + + name "Test Functions" + script "subworkflows/nf-core/utils_nextflow_pipeline/main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Test Function getWorkflowVersion") { + + function "getWorkflowVersion" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dumpParametersToJSON") { + + function "dumpParametersToJSON" + + when { + function { + """ + // define inputs of the function here. Example: + input[0] = "$outputDir" + """.stripIndent() + } + } + + then { + assertAll( + { assert function.success } + ) + } + } + + test("Test Function checkCondaChannels") { + + function "checkCondaChannels" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..db2030f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,12 @@ +{ + "Test Function getWorkflowVersion": { + "content": [ + "v9.9.9" + ], + "timestamp": "2024-01-19T11:32:36.031083" + }, + "Test Function checkCondaChannels": { + "content": null, + "timestamp": "2024-01-19T11:32:50.456" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..f7c54bc6 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,123 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NEXTFLOW_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config" + workflow "UTILS_NEXTFLOW_PIPELINE" + tag 'subworkflows' + tag 'utils_nextflow_pipeline' + tag 'subworkflows/utils_nextflow_pipeline' + + test("Should run no inputs") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = false + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should print version") { + + when { + params { + outdir = "tests/results" + } + workflow { + """ + print_version = true + dump_parameters = false + outdir = null + check_conda_channels = false + + input[0] = print_version + input[1] = dump_parameters + input[2] = outdir + input[3] = check_conda_channels + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.stdout.contains("nextflow_workflow v9.9.9") } + ) + } + } + + test("Should dump params") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = params.outdir + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should not create params JSON if no output directory") { + + when { + params { + outdir = "$outputDir" + } + workflow { + """ + print_version = false + dump_parameters = true + outdir = params.outdir + check_conda_channels = false + + input[0] = false + input[1] = true + input[2] = null + input[3] = false + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config new file mode 100644 index 00000000..53574ffe --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml new file mode 100644 index 00000000..f8476112 --- /dev/null +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nextflow_pipeline: + - subworkflows/nf-core/utils_nextflow_pipeline/** diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/main.nf b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf new file mode 100644 index 00000000..a8b55d6f --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/main.nf @@ -0,0 +1,440 @@ +// +// Subworkflow with utility functions specific to the nf-core pipeline template +// + +import org.yaml.snakeyaml.Yaml +import nextflow.extension.FilesEx + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFCORE_PIPELINE { + + take: + nextflow_cli_args + + main: + valid_config = checkConfigProvided() + checkProfileProvided(nextflow_cli_args) + + emit: + valid_config +} + +/* +======================================================================================== + FUNCTIONS +======================================================================================== +*/ + +// +// Warn if a -profile or Nextflow config has not been provided to run the pipeline +// +def checkConfigProvided() { + valid_config = true + if (workflow.profile == 'standard' && workflow.configFiles.size() <= 1) { + log.warn "[$workflow.manifest.name] You are attempting to run the pipeline without any custom configuration!\n\n" + + "This will be dependent on your local compute environment but can be achieved via one or more of the following:\n" + + " (1) Using an existing pipeline profile e.g. `-profile docker` or `-profile singularity`\n" + + " (2) Using an existing nf-core/configs for your Institution e.g. `-profile crick` or `-profile uppmax`\n" + + " (3) Using your own local custom config e.g. `-c /path/to/your/custom.config`\n\n" + + "Please refer to the quick start section and usage docs for the pipeline.\n " + valid_config = false + } + return valid_config +} + +// +// Exit pipeline if --profile contains spaces +// +def checkProfileProvided(nextflow_cli_args) { + if (workflow.profile.endsWith(',')) { + error "The `-profile` option cannot end with a trailing comma, please remove it and re-run the pipeline!\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } + if (nextflow_cli_args[0]) { + log.warn "nf-core pipelines do not accept positional arguments. The positional argument `${nextflow_cli_args[0]}` has been detected.\n" + + "HINT: A common mistake is to provide multiple values separated by spaces e.g. `-profile test, docker`.\n" + } +} + +// +// Citation string for pipeline +// +def workflowCitation() { + return "If you use ${workflow.manifest.name} for your analysis please cite:\n\n" + + "* The pipeline\n" + + " ${workflow.manifest.doi}\n\n" + + "* The nf-core framework\n" + + " https://doi.org/10.1038/s41587-020-0439-x\n\n" + + "* Software dependencies\n" + + " https://github.com/${workflow.manifest.name}/blob/master/CITATIONS.md" +} + +// +// Generate workflow version string +// +def getWorkflowVersion() { + String version_string = "" + if (workflow.manifest.version) { + def prefix_v = workflow.manifest.version[0] != 'v' ? 'v' : '' + version_string += "${prefix_v}${workflow.manifest.version}" + } + + if (workflow.commitId) { + def git_shortsha = workflow.commitId.substring(0, 7) + version_string += "-g${git_shortsha}" + } + + return version_string +} + +// +// Get software versions for pipeline +// +def processVersionsFromYAML(yaml_file) { + Yaml yaml = new Yaml() + versions = yaml.load(yaml_file).collectEntries { k, v -> [ k.tokenize(':')[-1], v ] } + return yaml.dumpAsMap(versions).trim() +} + +// +// Get workflow version for pipeline +// +def workflowVersionToYAML() { + return """ + Workflow: + $workflow.manifest.name: ${getWorkflowVersion()} + Nextflow: $workflow.nextflow.version + """.stripIndent().trim() +} + +// +// Get channel of software versions used in pipeline in YAML format +// +def softwareVersionsToYAML(ch_versions) { + return ch_versions + .unique() + .map { processVersionsFromYAML(it) } + .unique() + .mix(Channel.of(workflowVersionToYAML())) +} + +// +// Get workflow summary for MultiQC +// +def paramsSummaryMultiqc(summary_params) { + def summary_section = '' + for (group in summary_params.keySet()) { + def group_params = summary_params.get(group) // This gets the parameters of that particular group + if (group_params) { + summary_section += "

    $group

    \n" + summary_section += "
    \n" + for (param in group_params.keySet()) { + summary_section += "
    $param
    ${group_params.get(param) ?: 'N/A'}
    \n" + } + summary_section += "
    \n" + } + } + + String yaml_file_text = "id: '${workflow.manifest.name.replace('/','-')}-summary'\n" + yaml_file_text += "description: ' - this information is collected when the pipeline is started.'\n" + yaml_file_text += "section_name: '${workflow.manifest.name} Workflow Summary'\n" + yaml_file_text += "section_href: 'https://github.com/${workflow.manifest.name}'\n" + yaml_file_text += "plot_type: 'html'\n" + yaml_file_text += "data: |\n" + yaml_file_text += "${summary_section}" + + return yaml_file_text +} + +// +// nf-core logo +// +def nfCoreLogo(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + String.format( + """\n + ${dashedLine(monochrome_logs)} + ${colors.green},--.${colors.black}/${colors.green},-.${colors.reset} + ${colors.blue} ___ __ __ __ ___ ${colors.green}/,-._.--~\'${colors.reset} + ${colors.blue} |\\ | |__ __ / ` / \\ |__) |__ ${colors.yellow}} {${colors.reset} + ${colors.blue} | \\| | \\__, \\__/ | \\ |___ ${colors.green}\\`-._,-`-,${colors.reset} + ${colors.green}`._,._,\'${colors.reset} + ${colors.purple} ${workflow.manifest.name} ${getWorkflowVersion()}${colors.reset} + ${dashedLine(monochrome_logs)} + """.stripIndent() + ) +} + +// +// Return dashed line +// +def dashedLine(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + return "-${colors.dim}----------------------------------------------------${colors.reset}-" +} + +// +// ANSII colours used for terminal logging +// +def logColours(monochrome_logs=true) { + Map colorcodes = [:] + + // Reset / Meta + colorcodes['reset'] = monochrome_logs ? '' : "\033[0m" + colorcodes['bold'] = monochrome_logs ? '' : "\033[1m" + colorcodes['dim'] = monochrome_logs ? '' : "\033[2m" + colorcodes['underlined'] = monochrome_logs ? '' : "\033[4m" + colorcodes['blink'] = monochrome_logs ? '' : "\033[5m" + colorcodes['reverse'] = monochrome_logs ? '' : "\033[7m" + colorcodes['hidden'] = monochrome_logs ? '' : "\033[8m" + + // Regular Colors + colorcodes['black'] = monochrome_logs ? '' : "\033[0;30m" + colorcodes['red'] = monochrome_logs ? '' : "\033[0;31m" + colorcodes['green'] = monochrome_logs ? '' : "\033[0;32m" + colorcodes['yellow'] = monochrome_logs ? '' : "\033[0;33m" + colorcodes['blue'] = monochrome_logs ? '' : "\033[0;34m" + colorcodes['purple'] = monochrome_logs ? '' : "\033[0;35m" + colorcodes['cyan'] = monochrome_logs ? '' : "\033[0;36m" + colorcodes['white'] = monochrome_logs ? '' : "\033[0;37m" + + // Bold + colorcodes['bblack'] = monochrome_logs ? '' : "\033[1;30m" + colorcodes['bred'] = monochrome_logs ? '' : "\033[1;31m" + colorcodes['bgreen'] = monochrome_logs ? '' : "\033[1;32m" + colorcodes['byellow'] = monochrome_logs ? '' : "\033[1;33m" + colorcodes['bblue'] = monochrome_logs ? '' : "\033[1;34m" + colorcodes['bpurple'] = monochrome_logs ? '' : "\033[1;35m" + colorcodes['bcyan'] = monochrome_logs ? '' : "\033[1;36m" + colorcodes['bwhite'] = monochrome_logs ? '' : "\033[1;37m" + + // Underline + colorcodes['ublack'] = monochrome_logs ? '' : "\033[4;30m" + colorcodes['ured'] = monochrome_logs ? '' : "\033[4;31m" + colorcodes['ugreen'] = monochrome_logs ? '' : "\033[4;32m" + colorcodes['uyellow'] = monochrome_logs ? '' : "\033[4;33m" + colorcodes['ublue'] = monochrome_logs ? '' : "\033[4;34m" + colorcodes['upurple'] = monochrome_logs ? '' : "\033[4;35m" + colorcodes['ucyan'] = monochrome_logs ? '' : "\033[4;36m" + colorcodes['uwhite'] = monochrome_logs ? '' : "\033[4;37m" + + // High Intensity + colorcodes['iblack'] = monochrome_logs ? '' : "\033[0;90m" + colorcodes['ired'] = monochrome_logs ? '' : "\033[0;91m" + colorcodes['igreen'] = monochrome_logs ? '' : "\033[0;92m" + colorcodes['iyellow'] = monochrome_logs ? '' : "\033[0;93m" + colorcodes['iblue'] = monochrome_logs ? '' : "\033[0;94m" + colorcodes['ipurple'] = monochrome_logs ? '' : "\033[0;95m" + colorcodes['icyan'] = monochrome_logs ? '' : "\033[0;96m" + colorcodes['iwhite'] = monochrome_logs ? '' : "\033[0;97m" + + // Bold High Intensity + colorcodes['biblack'] = monochrome_logs ? '' : "\033[1;90m" + colorcodes['bired'] = monochrome_logs ? '' : "\033[1;91m" + colorcodes['bigreen'] = monochrome_logs ? '' : "\033[1;92m" + colorcodes['biyellow'] = monochrome_logs ? '' : "\033[1;93m" + colorcodes['biblue'] = monochrome_logs ? '' : "\033[1;94m" + colorcodes['bipurple'] = monochrome_logs ? '' : "\033[1;95m" + colorcodes['bicyan'] = monochrome_logs ? '' : "\033[1;96m" + colorcodes['biwhite'] = monochrome_logs ? '' : "\033[1;97m" + + return colorcodes +} + +// +// Attach the multiqc report to email +// +def attachMultiqcReport(multiqc_report) { + def mqc_report = null + try { + if (workflow.success) { + mqc_report = multiqc_report.getVal() + if (mqc_report.getClass() == ArrayList && mqc_report.size() >= 1) { + if (mqc_report.size() > 1) { + log.warn "[$workflow.manifest.name] Found multiple reports from process 'MULTIQC', will use only one" + } + mqc_report = mqc_report[0] + } + } + } catch (all) { + if (multiqc_report) { + log.warn "[$workflow.manifest.name] Could not attach MultiQC report to summary email" + } + } + return mqc_report +} + +// +// Construct and send completion email +// +def completionEmail(summary_params, email, email_on_fail, plaintext_email, outdir, monochrome_logs=true, multiqc_report=null) { + + // Set up the e-mail variables + def subject = "[$workflow.manifest.name] Successful: $workflow.runName" + if (!workflow.success) { + subject = "[$workflow.manifest.name] FAILED: $workflow.runName" + } + + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['Date Started'] = workflow.start + misc_fields['Date Completed'] = workflow.complete + misc_fields['Pipeline script file path'] = workflow.scriptFile + misc_fields['Pipeline script hash ID'] = workflow.scriptId + if (workflow.repository) misc_fields['Pipeline repository Git URL'] = workflow.repository + if (workflow.commitId) misc_fields['Pipeline repository Git Commit'] = workflow.commitId + if (workflow.revision) misc_fields['Pipeline Git branch/tag'] = workflow.revision + misc_fields['Nextflow Version'] = workflow.nextflow.version + misc_fields['Nextflow Build'] = workflow.nextflow.build + misc_fields['Nextflow Compile Timestamp'] = workflow.nextflow.timestamp + + def email_fields = [:] + email_fields['version'] = getWorkflowVersion() + email_fields['runName'] = workflow.runName + email_fields['success'] = workflow.success + email_fields['dateComplete'] = workflow.complete + email_fields['duration'] = workflow.duration + email_fields['exitStatus'] = workflow.exitStatus + email_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + email_fields['errorReport'] = (workflow.errorReport ?: 'None') + email_fields['commandLine'] = workflow.commandLine + email_fields['projectDir'] = workflow.projectDir + email_fields['summary'] = summary << misc_fields + + // On success try attach the multiqc report + def mqc_report = attachMultiqcReport(multiqc_report) + + // Check if we are only sending emails on failure + def email_address = email + if (!email && email_on_fail && !workflow.success) { + email_address = email_on_fail + } + + // Render the TXT template + def engine = new groovy.text.GStringTemplateEngine() + def tf = new File("${workflow.projectDir}/assets/email_template.txt") + def txt_template = engine.createTemplate(tf).make(email_fields) + def email_txt = txt_template.toString() + + // Render the HTML template + def hf = new File("${workflow.projectDir}/assets/email_template.html") + def html_template = engine.createTemplate(hf).make(email_fields) + def email_html = html_template.toString() + + // Render the sendmail template + def max_multiqc_email_size = (params.containsKey('max_multiqc_email_size') ? params.max_multiqc_email_size : 0) as nextflow.util.MemoryUnit + def smail_fields = [ email: email_address, subject: subject, email_txt: email_txt, email_html: email_html, projectDir: "${workflow.projectDir}", mqcFile: mqc_report, mqcMaxSize: max_multiqc_email_size.toBytes() ] + def sf = new File("${workflow.projectDir}/assets/sendmail_template.txt") + def sendmail_template = engine.createTemplate(sf).make(smail_fields) + def sendmail_html = sendmail_template.toString() + + // Send the HTML e-mail + Map colors = logColours(monochrome_logs) + if (email_address) { + try { + if (plaintext_email) { throw GroovyException('Send plaintext e-mail, not HTML') } + // Try to send HTML e-mail using sendmail + def sendmail_tf = new File(workflow.launchDir.toString(), ".sendmail_tmp.html") + sendmail_tf.withWriter { w -> w << sendmail_html } + [ 'sendmail', '-t' ].execute() << sendmail_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (sendmail)-" + } catch (all) { + // Catch failures and try with plaintext + def mail_cmd = [ 'mail', '-s', subject, '--content-type=text/html', email_address ] + mail_cmd.execute() << email_html + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Sent summary e-mail to $email_address (mail)-" + } + } + + // Write summary e-mail HTML to a file + def output_hf = new File(workflow.launchDir.toString(), ".pipeline_report.html") + output_hf.withWriter { w -> w << email_html } + FilesEx.copyTo(output_hf.toPath(), "${outdir}/pipeline_info/pipeline_report.html"); + output_hf.delete() + + // Write summary e-mail TXT to a file + def output_tf = new File(workflow.launchDir.toString(), ".pipeline_report.txt") + output_tf.withWriter { w -> w << email_txt } + FilesEx.copyTo(output_tf.toPath(), "${outdir}/pipeline_info/pipeline_report.txt"); + output_tf.delete() +} + +// +// Print pipeline summary on completion +// +def completionSummary(monochrome_logs=true) { + Map colors = logColours(monochrome_logs) + if (workflow.success) { + if (workflow.stats.ignoredCount == 0) { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.green} Pipeline completed successfully${colors.reset}-" + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.yellow} Pipeline completed successfully, but with errored process(es) ${colors.reset}-" + } + } else { + log.info "-${colors.purple}[$workflow.manifest.name]${colors.red} Pipeline completed with errors${colors.reset}-" + } +} + +// +// Construct and send a notification to a web server as JSON e.g. Microsoft Teams and Slack +// +def imNotification(summary_params, hook_url) { + def summary = [:] + for (group in summary_params.keySet()) { + summary << summary_params[group] + } + + def misc_fields = [:] + misc_fields['start'] = workflow.start + misc_fields['complete'] = workflow.complete + misc_fields['scriptfile'] = workflow.scriptFile + misc_fields['scriptid'] = workflow.scriptId + if (workflow.repository) misc_fields['repository'] = workflow.repository + if (workflow.commitId) misc_fields['commitid'] = workflow.commitId + if (workflow.revision) misc_fields['revision'] = workflow.revision + misc_fields['nxf_version'] = workflow.nextflow.version + misc_fields['nxf_build'] = workflow.nextflow.build + misc_fields['nxf_timestamp'] = workflow.nextflow.timestamp + + def msg_fields = [:] + msg_fields['version'] = getWorkflowVersion() + msg_fields['runName'] = workflow.runName + msg_fields['success'] = workflow.success + msg_fields['dateComplete'] = workflow.complete + msg_fields['duration'] = workflow.duration + msg_fields['exitStatus'] = workflow.exitStatus + msg_fields['errorMessage'] = (workflow.errorMessage ?: 'None') + msg_fields['errorReport'] = (workflow.errorReport ?: 'None') + msg_fields['commandLine'] = workflow.commandLine.replaceFirst(/ +--hook_url +[^ ]+/, "") + msg_fields['projectDir'] = workflow.projectDir + msg_fields['summary'] = summary << misc_fields + + // Render the JSON template + def engine = new groovy.text.GStringTemplateEngine() + // Different JSON depending on the service provider + // Defaults to "Adaptive Cards" (https://adaptivecards.io), except Slack which has its own format + def json_path = hook_url.contains("hooks.slack.com") ? "slackreport.json" : "adaptivecard.json" + def hf = new File("${workflow.projectDir}/assets/${json_path}") + def json_template = engine.createTemplate(hf).make(msg_fields) + def json_message = json_template.toString() + + // POST + def post = new URL(hook_url).openConnection(); + post.setRequestMethod("POST") + post.setDoOutput(true) + post.setRequestProperty("Content-Type", "application/json") + post.getOutputStream().write(json_message.getBytes("UTF-8")); + def postRC = post.getResponseCode(); + if (! postRC.equals(200)) { + log.warn(post.getErrorStream().getText()); + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml new file mode 100644 index 00000000..d08d2434 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/meta.yml @@ -0,0 +1,24 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFCORE_PIPELINE" +description: Subworkflow with utility functions specific to the nf-core pipeline template +keywords: + - utility + - pipeline + - initialise + - version +components: [] +input: + - nextflow_cli_args: + type: list + description: | + Nextflow CLI positional arguments +output: + - success: + type: boolean + description: | + Dummy output to indicate success +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test new file mode 100644 index 00000000..1dc317f8 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test @@ -0,0 +1,134 @@ + +nextflow_function { + + name "Test Functions" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Test Function checkConfigProvided") { + + function "checkConfigProvided" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function checkProfileProvided") { + + function "checkProfileProvided" + + when { + function { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function workflowCitation") { + + function "workflowCitation" + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function nfCoreLogo") { + + function "nfCoreLogo" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function dashedLine") { + + function "dashedLine" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function without logColours") { + + function "logColours" + + when { + function { + """ + input[0] = true + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } + + test("Test Function with logColours") { + function "logColours" + + when { + function { + """ + input[0] = false + """ + } + } + + then { + assertAll( + { assert function.success }, + { assert snapshot(function.result).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap new file mode 100644 index 00000000..10f948e6 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -0,0 +1,138 @@ +{ + "Test Function checkProfileProvided": { + "content": null, + "timestamp": "2024-02-09T15:43:55.145717" + }, + "Test Function checkConfigProvided": { + "content": [ + true + ], + "timestamp": "2024-01-19T11:34:13.548431224" + }, + "Test Function nfCoreLogo": { + "content": [ + "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" + ], + "timestamp": "2024-01-19T11:34:38.840454873" + }, + "Test Function workflowCitation": { + "content": [ + "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" + ], + "timestamp": "2024-01-19T11:34:22.24352016" + }, + "Test Function without logColours": { + "content": [ + { + "reset": "", + "bold": "", + "dim": "", + "underlined": "", + "blink": "", + "reverse": "", + "hidden": "", + "black": "", + "red": "", + "green": "", + "yellow": "", + "blue": "", + "purple": "", + "cyan": "", + "white": "", + "bblack": "", + "bred": "", + "bgreen": "", + "byellow": "", + "bblue": "", + "bpurple": "", + "bcyan": "", + "bwhite": "", + "ublack": "", + "ured": "", + "ugreen": "", + "uyellow": "", + "ublue": "", + "upurple": "", + "ucyan": "", + "uwhite": "", + "iblack": "", + "ired": "", + "igreen": "", + "iyellow": "", + "iblue": "", + "ipurple": "", + "icyan": "", + "iwhite": "", + "biblack": "", + "bired": "", + "bigreen": "", + "biyellow": "", + "biblue": "", + "bipurple": "", + "bicyan": "", + "biwhite": "" + } + ], + "timestamp": "2024-01-19T11:35:04.418416984" + }, + "Test Function dashedLine": { + "content": [ + "-\u001b[2m----------------------------------------------------\u001b[0m-" + ], + "timestamp": "2024-01-19T11:34:55.420000755" + }, + "Test Function with logColours": { + "content": [ + { + "reset": "\u001b[0m", + "bold": "\u001b[1m", + "dim": "\u001b[2m", + "underlined": "\u001b[4m", + "blink": "\u001b[5m", + "reverse": "\u001b[7m", + "hidden": "\u001b[8m", + "black": "\u001b[0;30m", + "red": "\u001b[0;31m", + "green": "\u001b[0;32m", + "yellow": "\u001b[0;33m", + "blue": "\u001b[0;34m", + "purple": "\u001b[0;35m", + "cyan": "\u001b[0;36m", + "white": "\u001b[0;37m", + "bblack": "\u001b[1;30m", + "bred": "\u001b[1;31m", + "bgreen": "\u001b[1;32m", + "byellow": "\u001b[1;33m", + "bblue": "\u001b[1;34m", + "bpurple": "\u001b[1;35m", + "bcyan": "\u001b[1;36m", + "bwhite": "\u001b[1;37m", + "ublack": "\u001b[4;30m", + "ured": "\u001b[4;31m", + "ugreen": "\u001b[4;32m", + "uyellow": "\u001b[4;33m", + "ublue": "\u001b[4;34m", + "upurple": "\u001b[4;35m", + "ucyan": "\u001b[4;36m", + "uwhite": "\u001b[4;37m", + "iblack": "\u001b[0;90m", + "ired": "\u001b[0;91m", + "igreen": "\u001b[0;92m", + "iyellow": "\u001b[0;93m", + "iblue": "\u001b[0;94m", + "ipurple": "\u001b[0;95m", + "icyan": "\u001b[0;96m", + "iwhite": "\u001b[0;97m", + "biblack": "\u001b[1;90m", + "bired": "\u001b[1;91m", + "bigreen": "\u001b[1;92m", + "biyellow": "\u001b[1;93m", + "biblue": "\u001b[1;94m", + "bipurple": "\u001b[1;95m", + "bicyan": "\u001b[1;96m", + "biwhite": "\u001b[1;97m" + } + ], + "timestamp": "2024-01-19T11:35:13.436366565" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test new file mode 100644 index 00000000..8940d32d --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test @@ -0,0 +1,29 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFCORE_PIPELINE" + script "../main.nf" + config "subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config" + workflow "UTILS_NFCORE_PIPELINE" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "utils_nfcore_pipeline" + tag "subworkflows/utils_nfcore_pipeline" + + test("Should run without failures") { + + when { + workflow { + """ + input[0] = [] + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert snapshot(workflow.out).match() } + ) + } + } +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap new file mode 100644 index 00000000..d07ce54c --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -0,0 +1,15 @@ +{ + "Should run without failures": { + "content": [ + { + "0": [ + true + ], + "valid_config": [ + true + ] + } + ], + "timestamp": "2024-01-19T11:35:22.538940073" + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config new file mode 100644 index 00000000..d0a926bf --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/nextflow.config @@ -0,0 +1,9 @@ +manifest { + name = 'nextflow_workflow' + author = """nf-core""" + homePage = 'https://127.0.0.1' + description = """Dummy pipeline""" + nextflowVersion = '!>=23.04.0' + version = '9.9.9' + doi = 'https://doi.org/10.5281/zenodo.5070524' +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml new file mode 100644 index 00000000..ac8523c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfcore_pipeline: + - subworkflows/nf-core/utils_nfcore_pipeline/** diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf new file mode 100644 index 00000000..2585b65d --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/main.nf @@ -0,0 +1,62 @@ +// +// Subworkflow that uses the nf-validation plugin to render help text and parameter summary +// + +/* +======================================================================================== + IMPORT NF-VALIDATION PLUGIN +======================================================================================== +*/ + +include { paramsHelp } from 'plugin/nf-validation' +include { paramsSummaryLog } from 'plugin/nf-validation' +include { validateParameters } from 'plugin/nf-validation' + +/* +======================================================================================== + SUBWORKFLOW DEFINITION +======================================================================================== +*/ + +workflow UTILS_NFVALIDATION_PLUGIN { + + take: + print_help // boolean: print help + workflow_command // string: default commmand used to run pipeline + pre_help_text // string: string to be printed before help text and summary log + post_help_text // string: string to be printed after help text and summary log + validate_params // boolean: validate parameters + schema_filename // path: JSON schema file, null to use default value + + main: + + log.debug "Using schema file: ${schema_filename}" + + // Default values for strings + pre_help_text = pre_help_text ?: '' + post_help_text = post_help_text ?: '' + workflow_command = workflow_command ?: '' + + // + // Print help message if needed + // + if (print_help) { + log.info pre_help_text + paramsHelp(workflow_command, parameters_schema: schema_filename) + post_help_text + System.exit(0) + } + + // + // Print parameter summary to stdout + // + log.info pre_help_text + paramsSummaryLog(workflow, parameters_schema: schema_filename) + post_help_text + + // + // Validate parameters relative to the parameter JSON schema + // + if (validate_params){ + validateParameters(parameters_schema: schema_filename) + } + + emit: + dummy_emit = true +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml new file mode 100644 index 00000000..3d4a6b04 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/meta.yml @@ -0,0 +1,44 @@ +# yaml-language-server: $schema=https://raw.githubusercontent.com/nf-core/modules/master/subworkflows/yaml-schema.json +name: "UTILS_NFVALIDATION_PLUGIN" +description: Use nf-validation to initiate and validate a pipeline +keywords: + - utility + - pipeline + - initialise + - validation +components: [] +input: + - print_help: + type: boolean + description: | + Print help message and exit + - workflow_command: + type: string + description: | + The command to run the workflow e.g. "nextflow run main.nf" + - pre_help_text: + type: string + description: | + Text to print before the help message + - post_help_text: + type: string + description: | + Text to print after the help message + - validate_params: + type: boolean + description: | + Validate the parameters and error if invalid. + - schema_filename: + type: string + description: | + The filename of the schema to validate against. +output: + - dummy_emit: + type: boolean + description: | + Dummy emit to make nf-core subworkflows lint happy +authors: + - "@adamrtalbot" +maintainers: + - "@adamrtalbot" + - "@maxulysse" diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test new file mode 100644 index 00000000..517ee54e --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -0,0 +1,200 @@ +nextflow_workflow { + + name "Test Workflow UTILS_NFVALIDATION_PLUGIN" + script "../main.nf" + workflow "UTILS_NFVALIDATION_PLUGIN" + tag "subworkflows" + tag "subworkflows_nfcore" + tag "plugin/nf-validation" + tag "'plugin/nf-validation'" + tag "utils_nfvalidation_plugin" + tag "subworkflows/utils_nfvalidation_plugin" + + test("Should run nothing") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success } + ) + } + } + + test("Should run help") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with command") { + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = null + post_help_text = null + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } } + ) + } + } + + test("Should run help with extra text") { + + + when { + + params { + monochrome_logs = true + test_data = '' + } + workflow { + """ + help = true + workflow_command = "nextflow run noorg/doesntexist" + pre_help_text = "pre-help-text" + post_help_text = "post-help-text" + validate_params = false + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.success }, + { assert workflow.exitStatus == 0 }, + { assert workflow.stdout.any { it.contains('pre-help-text') } }, + { assert workflow.stdout.any { it.contains('nextflow run noorg/doesntexist') } }, + { assert workflow.stdout.any { it.contains('Input/output options') } }, + { assert workflow.stdout.any { it.contains('--outdir') } }, + { assert workflow.stdout.any { it.contains('post-help-text') } } + ) + } + } + + test("Should validate params") { + + when { + + params { + monochrome_logs = true + test_data = '' + outdir = 1 + } + workflow { + """ + help = false + workflow_command = null + pre_help_text = null + post_help_text = null + validate_params = true + schema_filename = "$moduleTestDir/nextflow_schema.json" + + input[0] = help + input[1] = workflow_command + input[2] = pre_help_text + input[3] = post_help_text + input[4] = validate_params + input[5] = schema_filename + """ + } + } + + then { + assertAll( + { assert workflow.failed }, + { assert workflow.stdout.any { it.contains('ERROR ~ ERROR: Validation of pipeline parameters failed!') } } + ) + } + } +} \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json new file mode 100644 index 00000000..7626c1c9 --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/nextflow_schema.json @@ -0,0 +1,96 @@ +{ + "$schema": "http://json-schema.org/draft-07/schema", + "$id": "https://raw.githubusercontent.com/./master/nextflow_schema.json", + "title": ". pipeline parameters", + "description": "", + "type": "object", + "definitions": { + "input_output_options": { + "title": "Input/output options", + "type": "object", + "fa_icon": "fas fa-terminal", + "description": "Define where the pipeline should find input data and save output data.", + "required": ["outdir"], + "properties": { + "validate_params": { + "type": "boolean", + "description": "Validate parameters?", + "default": true, + "hidden": true + }, + "outdir": { + "type": "string", + "format": "directory-path", + "description": "The output directory where the results will be saved. You have to use absolute paths to storage on Cloud infrastructure.", + "fa_icon": "fas fa-folder-open" + }, + "test_data_base": { + "type": "string", + "default": "https://raw.githubusercontent.com/nf-core/test-datasets/modules", + "description": "Base for test data directory", + "hidden": true + }, + "test_data": { + "type": "string", + "description": "Fake test data param", + "hidden": true + } + } + }, + "generic_options": { + "title": "Generic options", + "type": "object", + "fa_icon": "fas fa-file-import", + "description": "Less common options for the pipeline, typically set in a config file.", + "help_text": "These options are common to all nf-core pipelines and allow you to customise some of the core preferences for how the pipeline runs.\n\nTypically these options would be set in a Nextflow config file loaded for all pipeline runs, such as `~/.nextflow/config`.", + "properties": { + "help": { + "type": "boolean", + "description": "Display help text.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "version": { + "type": "boolean", + "description": "Display version and exit.", + "fa_icon": "fas fa-question-circle", + "hidden": true + }, + "logo": { + "type": "boolean", + "default": true, + "description": "Display nf-core logo in console output.", + "fa_icon": "fas fa-image", + "hidden": true + }, + "singularity_pull_docker_container": { + "type": "boolean", + "description": "Pull Singularity container from Docker?", + "hidden": true + }, + "publish_dir_mode": { + "type": "string", + "default": "copy", + "description": "Method used to save pipeline results to output directory.", + "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", + "fa_icon": "fas fa-copy", + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "hidden": true + }, + "monochrome_logs": { + "type": "boolean", + "description": "Use monochrome_logs", + "hidden": true + } + } + } + }, + "allOf": [ + { + "$ref": "#/definitions/input_output_options" + }, + { + "$ref": "#/definitions/generic_options" + } + ] +} diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml new file mode 100644 index 00000000..60b1cfff --- /dev/null +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/tags.yml @@ -0,0 +1,2 @@ +subworkflows/utils_nfvalidation_plugin: + - subworkflows/nf-core/utils_nfvalidation_plugin/** diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 115b6b48..40e0dfdb 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -1,54 +1,15 @@ /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - PRINT PARAMS SUMMARY + IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - -def logo = NfcoreTemplate.logo(workflow, params.monochrome_logs) -def citation = '\n' + WorkflowMain.citation(workflow) + '\n' -def summary_params = paramsSummaryMap(workflow) - -// Print parameter summary log to screen -log.info logo + paramsSummaryLog(workflow) + citation - -WorkflowScrnaseq.initialise(params, log) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' +include { FASTQC } from '../modules/nf-core/fastqc/main' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_scrnaseq_pipeline' /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -56,50 +17,45 @@ include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoft ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -// Info required for completion email and summary -def multiqc_report = [] - workflow SCRNASEQ { - ch_versions = Channel.empty() + take: + ch_samplesheet // channel: samplesheet read in from --input - // - // SUBWORKFLOW: Read in samplesheet, validate and stage input files - // - INPUT_CHECK ( - file(params.input) - ) - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema + main: + + ch_versions = Channel.empty() + ch_multiqc_files = Channel.empty() // // MODULE: Run FastQC // FASTQC ( - INPUT_CHECK.out.reads + ch_samplesheet ) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}) ch_versions = ch_versions.mix(FASTQC.out.versions.first()) - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) + // + // Collate and save software versions + // + softwareVersionsToYAML(ch_versions) + .collectFile(storeDir: "${params.outdir}/pipeline_info", name: 'nf_core_pipeline_software_mqc_versions.yml', sort: true, newLine: true) + .set { ch_collated_versions } // // MODULE: MultiQC // - workflow_summary = WorkflowScrnaseq.paramsSummaryMultiqc(workflow, summary_params) - ch_workflow_summary = Channel.value(workflow_summary) - - methods_description = WorkflowScrnaseq.methodsDescriptionText(workflow, ch_multiqc_custom_methods_description, params) - ch_methods_description = Channel.value(methods_description) - - ch_multiqc_files = Channel.empty() - ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(CUSTOM_DUMPSOFTWAREVERSIONS.out.mqc_yml.collect()) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC.out.zip.collect{it[1]}.ifEmpty([])) + ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) + ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath(params.multiqc_config, checkIfExists: true) : Channel.empty() + ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath(params.multiqc_logo, checkIfExists: true) : Channel.empty() + summary_params = paramsSummaryMap(workflow, parameters_schema: "nextflow_schema.json") + ch_workflow_summary = Channel.value(paramsSummaryMultiqc(summary_params)) + ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) + ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) + ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) + ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( ch_multiqc_files.collect(), @@ -107,31 +63,10 @@ workflow SCRNASEQ { ch_multiqc_custom_config.toList(), ch_multiqc_logo.toList() ) - multiqc_report = MULTIQC.out.report.toList() -} - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - COMPLETION EMAIL AND SUMMARY -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -workflow.onComplete { - if (params.email || params.email_on_fail) { - NfcoreTemplate.email(workflow, params, summary_params, projectDir, log, multiqc_report) - } - NfcoreTemplate.dump_parameters(workflow, params) - NfcoreTemplate.summary(workflow, params, log) - if (params.hook_url) { - NfcoreTemplate.IM_notification(workflow, params, summary_params, projectDir, log) - } -} -workflow.onError { - if (workflow.errorReport.contains("Process requirement exceeds available memory")) { - println("🛑 Default resources exceed availability 🛑 ") - println("💡 See here on how to configure pipeline: https://nf-co.re/docs/usage/configuration#tuning-workflow-resources 💡") - } + emit: + multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html + versions = ch_versions // channel: [ path(versions.yml) ] } /* From bf276856b4159b0da100f33f1c1cb5f8a792e0a6 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Fri, 23 Feb 2024 11:32:52 -0800 Subject: [PATCH 052/111] harmonizing with PR while that's in progress --- modules/nf-core/kallistobustools/count/main.nf | 4 ++-- modules/nf-core/kallistobustools/count/meta.yml | 4 ++-- .../nf-core/kallistobustools/ref/environment.yml | 3 ++- modules/nf-core/kallistobustools/ref/main.nf | 8 ++++---- modules/nf-core/kallistobustools/ref/meta.yml | 14 +++++++------- 5 files changed, 17 insertions(+), 16 deletions(-) diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index 9e860241..ce5b5365 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -27,7 +27,7 @@ process KALLISTOBUSTOOLS_COUNT { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def cdna = t1c ? "-c1 $t1c" : '' - def unprocessed = t2c ? "-c2 $t2c" : '' + def intron = t2c ? "-c2 $t2c" : '' def memory = task.memory.toGiga() - 1 """ kb \\ @@ -36,7 +36,7 @@ process KALLISTOBUSTOOLS_COUNT { -i $index \\ -g $t2g \\ $cdna \\ - $unprocessed \\ + $intron \\ -x $technology \\ $args \\ -o ${prefix}.count \\ diff --git a/modules/nf-core/kallistobustools/count/meta.yml b/modules/nf-core/kallistobustools/count/meta.yml index c8763343..4a5d4b88 100644 --- a/modules/nf-core/kallistobustools/count/meta.yml +++ b/modules/nf-core/kallistobustools/count/meta.yml @@ -38,8 +38,8 @@ input: pattern: "*.{cdna_t2c.txt}" - t2c: type: file - description: kb ref's c2 unprocessed_t2c file - pattern: "*.{unprocessed_t2c.txt}" + description: kb ref's c2 intron_t2c file + pattern: "*.{intron_t2c.txt}" - workflow_mode: type: string description: String value defining workflow to use, can be one of "standard", "nac", "lamanno" (obsolete) diff --git a/modules/nf-core/kallistobustools/ref/environment.yml b/modules/nf-core/kallistobustools/ref/environment.yml index 62942e01..82aafedb 100644 --- a/modules/nf-core/kallistobustools/ref/environment.yml +++ b/modules/nf-core/kallistobustools/ref/environment.yml @@ -5,4 +5,5 @@ channels: - defaults dependencies: - bioconda::kb-python=0.28.2 - - conda-forge::requests>=2.23.0 \ No newline at end of file + - conda-forge::requests>=2.23.0 + \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/ref/main.nf b/modules/nf-core/kallistobustools/ref/main.nf index 989f86f3..759e8663 100644 --- a/modules/nf-core/kallistobustools/ref/main.nf +++ b/modules/nf-core/kallistobustools/ref/main.nf @@ -17,9 +17,9 @@ process KALLISTOBUSTOOLS_REF { path "kb_ref_out.idx" , emit: index path "t2g.txt" , emit: t2g path "cdna.fa" , emit: cdna - path "unprocessed.fa" , optional:true, emit: unprocessed + path "intron.fa" , optional:true, emit: intron path "cdna_t2c.txt" , optional:true, emit: cdna_t2c - path "unprocessed_t2c.txt" , optional:true, emit: unprocessed_t2c + path "intron_t2c.txt" , optional:true, emit: intron_t2c when: task.ext.when == null || task.ext.when @@ -49,9 +49,9 @@ process KALLISTOBUSTOOLS_REF { -i kb_ref_out.idx \\ -g t2g.txt \\ -f1 cdna.fa \\ - -f2 unprocessed.fa \\ + -f2 intron.fa \\ -c1 cdna_t2c.txt \\ - -c2 unprocessed_t2c.txt \\ + -c2 intron_t2c.txt \\ --workflow $workflow_mode \\ $fasta \\ $gtf diff --git a/modules/nf-core/kallistobustools/ref/meta.yml b/modules/nf-core/kallistobustools/ref/meta.yml index 1ca31c57..0c3f78a4 100644 --- a/modules/nf-core/kallistobustools/ref/meta.yml +++ b/modules/nf-core/kallistobustools/ref/meta.yml @@ -45,19 +45,19 @@ output: type: file description: cDNA fasta file pattern: "*cdna.{fa}" - - unprocessed: + - intron: type: file - description: Unprocessed fasta file - pattern: "*unprocessed.{fa}" + description: Intron fasta file + pattern: "*intron.{fa}" - cdna_t2c: type: file description: cDNA transcript to capture file pattern: "*cdna_t2c.{txt}" - - unprocessed_t2c: + - intron_t2c: type: file - description: Unprocessed transcript to capture file - pattern: "*unprocessed_t2c.{txt}" + description: Intron transcript to capture file + pattern: "*intron_t2c.{txt}" authors: - - "@flowuenne" + - "@flowuenne" maintainers: - "@flowuenne" From ac7e7cdfe24cf39e9b432d21e1a077e45da39684 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Fri, 23 Feb 2024 13:00:01 -0800 Subject: [PATCH 053/111] Update nextflow_schema.json --- nextflow_schema.json | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 10087c2c..2d204477 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -220,9 +220,9 @@ "kb_workflow": { "type": "string", "default": "standard", - "description": "Type of workflow. Use `lamanno` for RNA velocity based on La Manno et al. 2018 logic. Use `nucleus` for RNA velocity on single-nucleus RNA-seq reads. Use `kite` for feature barcoding. Use `kite: 10xFB` for 10x Genomics Feature Barcoding technology. (default: standard)", + "description": "Type of workflow. Use `nac` for an index type that can quantify nascent and mature RNA. Use `lamanno` for RNA velocity based on La Manno et al. 2018 logic. (default: standard)", "fa_icon": "fas fa-rainbow", - "enum": ["standard", "lamanno", "nucleus", "kite", "kite: 10xFB"] + "enum": ["standard", "lamanno", "nac"] } } }, From 8c4b8723b298a8f02c291e2f011f7e39660a0d11 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Fri, 23 Feb 2024 14:35:01 -0800 Subject: [PATCH 054/111] Update main.nf --- modules/nf-core/kallistobustools/count/main.nf | 2 ++ 1 file changed, 2 insertions(+) diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index ce5b5365..4679fe97 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -14,6 +14,7 @@ process KALLISTOBUSTOOLS_COUNT { path t1c path t2c val technology + val workflow_mode output: tuple val(meta), path ("*.count"), emit: count @@ -38,6 +39,7 @@ process KALLISTOBUSTOOLS_COUNT { $cdna \\ $intron \\ -x $technology \\ + --workflow $workflow_mode \\ $args \\ -o ${prefix}.count \\ -m ${memory}G \\ From 8db2f9553da65c4814523a1bd548a41dc50b72f2 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Fri, 23 Feb 2024 16:03:08 -0800 Subject: [PATCH 055/111] Update kallisto_bustools.nf --- subworkflows/local/kallisto_bustools.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/subworkflows/local/kallisto_bustools.nf b/subworkflows/local/kallisto_bustools.nf index 7fdaf8ef..8b16dc56 100644 --- a/subworkflows/local/kallisto_bustools.nf +++ b/subworkflows/local/kallisto_bustools.nf @@ -34,7 +34,7 @@ workflow KALLISTO_BUSTOOLS { kallisto_index = KALLISTOBUSTOOLS_REF.out.index.collect() ch_versions = ch_versions.mix(KALLISTOBUSTOOLS_REF.out.versions) t1c = KALLISTOBUSTOOLS_REF.out.cdna_t2c.ifEmpty{ [] } - t2c = KALLISTOBUSTOOLS_REF.out.unprocessed_t2c.ifEmpty{ [] } + t2c = KALLISTOBUSTOOLS_REF.out.intron_t2c.ifEmpty{ [] } } /* From a1fdcda8a13ade6c5218cd11f9068ab609b45746 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Fri, 23 Feb 2024 16:09:08 -0800 Subject: [PATCH 056/111] Update kallisto_bustools.nf --- subworkflows/local/kallisto_bustools.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/kallisto_bustools.nf b/subworkflows/local/kallisto_bustools.nf index 8b16dc56..b6549094 100644 --- a/subworkflows/local/kallisto_bustools.nf +++ b/subworkflows/local/kallisto_bustools.nf @@ -46,7 +46,8 @@ workflow KALLISTO_BUSTOOLS { txp2gene, t1c, t2c, - protocol + protocol, + kb_workflow ) ch_versions = ch_versions.mix(KALLISTOBUSTOOLS_COUNT.out.versions) From 2aca75b5df3c90dc3284995b2c1713b9c8ec7747 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 11:39:50 -0800 Subject: [PATCH 057/111] docker container update --- modules/nf-core/kallistobustools/count/main.nf | 4 ++-- modules/nf-core/kallistobustools/count/meta.yml | 2 +- modules/nf-core/kallistobustools/ref/environment.yml | 1 - modules/nf-core/kallistobustools/ref/main.nf | 4 ++-- modules/nf-core/kallistobustools/ref/meta.yml | 2 +- 5 files changed, 6 insertions(+), 7 deletions(-) diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index 4679fe97..67ad5812 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -4,8 +4,8 @@ process KALLISTOBUSTOOLS_COUNT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/kb-python:0.28.2--pyhdfd78af_0' : - 'biocontainers/kb-python:0.28.2--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/kb-python:0.28.2--pyhdfd78af_2' : + 'biocontainers/kb-python:0.28.2--pyhdfd78af_2' }" input: tuple val(meta), path(reads) diff --git a/modules/nf-core/kallistobustools/count/meta.yml b/modules/nf-core/kallistobustools/count/meta.yml index 4a5d4b88..55d5dc6c 100644 --- a/modules/nf-core/kallistobustools/count/meta.yml +++ b/modules/nf-core/kallistobustools/count/meta.yml @@ -12,7 +12,7 @@ tools: homepage: https://www.kallistobus.tools/ documentation: https://kb-python.readthedocs.io/en/latest/index.html tool_dev_url: https://github.com/pachterlab/kb_python - licence: MIT License + licence: ["MIT"] input: - meta: type: map diff --git a/modules/nf-core/kallistobustools/ref/environment.yml b/modules/nf-core/kallistobustools/ref/environment.yml index 82aafedb..6ae07a8c 100644 --- a/modules/nf-core/kallistobustools/ref/environment.yml +++ b/modules/nf-core/kallistobustools/ref/environment.yml @@ -6,4 +6,3 @@ channels: dependencies: - bioconda::kb-python=0.28.2 - conda-forge::requests>=2.23.0 - \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/ref/main.nf b/modules/nf-core/kallistobustools/ref/main.nf index 759e8663..702317d3 100644 --- a/modules/nf-core/kallistobustools/ref/main.nf +++ b/modules/nf-core/kallistobustools/ref/main.nf @@ -4,8 +4,8 @@ process KALLISTOBUSTOOLS_REF { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/kb-python:0.28.2--pyhdfd78af_0' : - 'biocontainers/kb-python:0.28.2--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/kb-python:0.28.2--pyhdfd78af_2' : + 'biocontainers/kb-python:0.28.2--pyhdfd78af_2' }" input: path fasta diff --git a/modules/nf-core/kallistobustools/ref/meta.yml b/modules/nf-core/kallistobustools/ref/meta.yml index 0c3f78a4..64deab9b 100644 --- a/modules/nf-core/kallistobustools/ref/meta.yml +++ b/modules/nf-core/kallistobustools/ref/meta.yml @@ -14,7 +14,7 @@ tools: documentation: https://kb-python.readthedocs.io/en/latest/index.html tool_dev_url: https://github.com/pachterlab/kb_python doi: "10.22002/D1.1876" - licence: MIT License + licence: ["MIT"] input: - fasta: type: file From 0f5609325d0a55adb6e7a3283bedba07e7912873 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 12:05:48 -0800 Subject: [PATCH 058/111] manually updated md5s this is not a real snapshot --- tests/main_pipeline_kallisto.test.snap | 16 +++++++++------- 1 file changed, 9 insertions(+), 7 deletions(-) diff --git a/tests/main_pipeline_kallisto.test.snap b/tests/main_pipeline_kallisto.test.snap index b0fb50bb..94a64ad8 100644 --- a/tests/main_pipeline_kallisto.test.snap +++ b/tests/main_pipeline_kallisto.test.snap @@ -20,15 +20,17 @@ "name": "workflow", "success": true }, - "cells_x_genes.barcodes.txt:md5,18be561873e435d4587f6b3f95a0e301", + "cells_x_genes.barcodes.txt:md5,72d78bb1c1ee7cb174520b30f695aa48", + "cells_x_genes.genes.names.txt:md5,a27831417ff4ed049d5b46012b270f4d", "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", - "cells_x_genes.mtx:md5,37d2cd8c712f9c70463e87485bf6cd36", - "cells_x_genes.barcodes.txt:md5,488437e1f5477243697efb93366e5676", + "cells_x_genes.mtx:md5,894d60da192e3788de11fa8fc1fa711d", + "cells_x_genes.barcodes.txt:md5,a8cf7ea4b2d075296a94bf066a64b7a4", + "cells_x_genes.genes.names.txt:md5,a27831417ff4ed049d5b46012b270f4d", "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", - "cells_x_genes.mtx:md5,af90e05b404490f6cb133ab7f62949f8", - "Sample_X_matrix.rds:md5,f0e43f69403f4b2e7704065421592ad0", - "Sample_Y_matrix.rds:md5,61809156e64dbdaf254cbc1c3456588e" + "cells_x_genes.mtx:md5,abd83de117204d0a77df3c92d00cc025", + "Sample_X_matrix.rds:md5,294781892964ed3c06f5c02124bb17b3", + "Sample_Y_matrix.rds:md5,b950e59c61ad3fdeb7293a1e5bfcd313" ], - "timestamp": "2024-01-23T12:19:47.921508953" + "timestamp": "2024-02-27T12:19:47.921508953" } } \ No newline at end of file From aae5fc5f618d1a4ccd40428d14050d838876f7fe Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 12:19:56 -0800 Subject: [PATCH 059/111] Update main_pipeline_kallisto.test.snap --- tests/main_pipeline_kallisto.test.snap | 2 -- 1 file changed, 2 deletions(-) diff --git a/tests/main_pipeline_kallisto.test.snap b/tests/main_pipeline_kallisto.test.snap index 94a64ad8..867947ec 100644 --- a/tests/main_pipeline_kallisto.test.snap +++ b/tests/main_pipeline_kallisto.test.snap @@ -21,11 +21,9 @@ "success": true }, "cells_x_genes.barcodes.txt:md5,72d78bb1c1ee7cb174520b30f695aa48", - "cells_x_genes.genes.names.txt:md5,a27831417ff4ed049d5b46012b270f4d", "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", "cells_x_genes.mtx:md5,894d60da192e3788de11fa8fc1fa711d", "cells_x_genes.barcodes.txt:md5,a8cf7ea4b2d075296a94bf066a64b7a4", - "cells_x_genes.genes.names.txt:md5,a27831417ff4ed049d5b46012b270f4d", "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", "cells_x_genes.mtx:md5,abd83de117204d0a77df3c92d00cc025", "Sample_X_matrix.rds:md5,294781892964ed3c06f5c02124bb17b3", From 740a5dc6254f122b94816b43706cf84cd94cf189 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 13:01:57 -0800 Subject: [PATCH 060/111] update mtx construction mtx should take in nascent/ambiguous/mature matrices. The genes.names txt contains gene names, which are typically what is desired (e.g. cellranger has names rather than IDs) --- modules/local/mtx_to_h5ad.nf | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 84d98608..043febf1 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -27,7 +27,7 @@ process MTX_TO_H5AD { if (params.aligner == 'kallisto') { mtx_matrix = "*count/counts_unfiltered/*.mtx" barcodes_tsv = "*count/counts_unfiltered/*.barcodes.txt" - features_tsv = "*count/counts_unfiltered/*.genes.txt" + features_tsv = "*count/counts_unfiltered/*.genes.names.txt" } else if (params.aligner == 'alevin') { mtx_matrix = "*_alevin_results/af_quant/alevin/quants_mat.mtx" barcodes_tsv = "*_alevin_results/af_quant/alevin/quants_mat_rows.txt" @@ -54,13 +54,13 @@ process MTX_TO_H5AD { else if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') """ # convert file types - for input_type in spliced unspliced ; do + for input_type in nascent ambiguous mature ; do mtx_to_h5ad.py \\ --aligner ${params.aligner} \\ --sample ${meta.id} \\ - --input *count/counts_unfiltered/\${input_type}.mtx \\ - --barcode *count/counts_unfiltered/\${input_type}.barcodes.txt \\ - --feature *count/counts_unfiltered/\${input_type}.genes.txt \\ + --input *count/counts_unfiltered/cells_x_genes.\${input_type}.mtx \\ + --barcode *count/counts_unfiltered/cells_x_genes.\${input_type}.barcodes.txt \\ + --feature *count/counts_unfiltered/cells_x_genes.\${input_type}.genes.names.txt \\ --txp2gene ${txp2gene} \\ --star_index ${star_index} \\ --out ${meta.id}/${meta.id}_\${input_type}_matrix.h5ad ; From e7f59949be73f0a045d16ab266b390a9c5bab95e Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 13:22:22 -0800 Subject: [PATCH 061/111] update seurat too --- modules/local/mtx_to_seurat.nf | 10 +++++----- 1 file changed, 5 insertions(+), 5 deletions(-) diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index d83575a4..04710f37 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -26,7 +26,7 @@ process MTX_TO_SEURAT { } else if (params.aligner == "kallisto") { matrix = "*count/counts_unfiltered/*.mtx" barcodes = "*count/counts_unfiltered/*.barcodes.txt" - features = "*count/counts_unfiltered/*.genes.txt" + features = "*count/counts_unfiltered/*.genes.names.txt" } else if (params.aligner == "alevin") { matrix = "*_alevin_results/af_quant/alevin/quants_mat.mtx" barcodes = "*_alevin_results/af_quant/alevin/quants_mat_rows.txt" @@ -43,11 +43,11 @@ process MTX_TO_SEURAT { if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') """ # convert file types - for input_type in spliced unspliced ; do + for input_type in nascent ambiguous mature ; do mtx_to_seurat.R \\ - *count/counts_unfiltered/\${input_type}.mtx \\ - *count/counts_unfiltered/\${input_type}.barcodes.txt \\ - *count/counts_unfiltered/\${input_type}.genes.txt \\ + *count/counts_unfiltered/cells_x_genes.\${input_type}.mtx \\ + *count/counts_unfiltered/cells_x_genes.\${input_type}.barcodes.txt \\ + *count/counts_unfiltered/cells_x_genes.\${input_type}.genes.names.txt \\ ${meta.id}/${meta.id}_\${input_type}_matrix.rds \\ ${aligner} done From 25ecada467c7a43d9b6450d25523f4d06fcadff2 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 13:40:33 -0800 Subject: [PATCH 062/111] this must have gotten fixed a few kb versions ago --- modules/local/mtx_to_h5ad.nf | 2 +- modules/local/mtx_to_seurat.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 043febf1..7bde6e79 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -59,7 +59,7 @@ process MTX_TO_H5AD { --aligner ${params.aligner} \\ --sample ${meta.id} \\ --input *count/counts_unfiltered/cells_x_genes.\${input_type}.mtx \\ - --barcode *count/counts_unfiltered/cells_x_genes.\${input_type}.barcodes.txt \\ + --barcode $barcodes_tsv \\ --feature *count/counts_unfiltered/cells_x_genes.\${input_type}.genes.names.txt \\ --txp2gene ${txp2gene} \\ --star_index ${star_index} \\ diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index 04710f37..a9ff508f 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -46,7 +46,7 @@ process MTX_TO_SEURAT { for input_type in nascent ambiguous mature ; do mtx_to_seurat.R \\ *count/counts_unfiltered/cells_x_genes.\${input_type}.mtx \\ - *count/counts_unfiltered/cells_x_genes.\${input_type}.barcodes.txt \\ + $barcodes_tsv \\ *count/counts_unfiltered/cells_x_genes.\${input_type}.genes.names.txt \\ ${meta.id}/${meta.id}_\${input_type}_matrix.rds \\ ${aligner} From 072d187a29b1cd6c5f81bfa599b64920e0599ebb Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 13:49:26 -0800 Subject: [PATCH 063/111] ...and the R changes have to be updated in md5 --- tests/main_pipeline_kallisto.test.snap | 4 ++-- 1 file changed, 2 insertions(+), 2 deletions(-) diff --git a/tests/main_pipeline_kallisto.test.snap b/tests/main_pipeline_kallisto.test.snap index 867947ec..00fbb2b2 100644 --- a/tests/main_pipeline_kallisto.test.snap +++ b/tests/main_pipeline_kallisto.test.snap @@ -26,8 +26,8 @@ "cells_x_genes.barcodes.txt:md5,a8cf7ea4b2d075296a94bf066a64b7a4", "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", "cells_x_genes.mtx:md5,abd83de117204d0a77df3c92d00cc025", - "Sample_X_matrix.rds:md5,294781892964ed3c06f5c02124bb17b3", - "Sample_Y_matrix.rds:md5,b950e59c61ad3fdeb7293a1e5bfcd313" + "Sample_X_matrix.rds:md5,0938f4189b7a7fd1030abfcee798741c", + "Sample_Y_matrix.rds:md5,93c12abe283ab37c5f37e5cd3cb25302" ], "timestamp": "2024-02-27T12:19:47.921508953" } From e006cdf2255ca793b303f7072b8c34687ac70909 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 13:53:28 -0800 Subject: [PATCH 064/111] correcting var --- modules/local/mtx_to_seurat.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index a9ff508f..ac21e639 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -46,7 +46,7 @@ process MTX_TO_SEURAT { for input_type in nascent ambiguous mature ; do mtx_to_seurat.R \\ *count/counts_unfiltered/cells_x_genes.\${input_type}.mtx \\ - $barcodes_tsv \\ + $barcodes \\ *count/counts_unfiltered/cells_x_genes.\${input_type}.genes.names.txt \\ ${meta.id}/${meta.id}_\${input_type}_matrix.rds \\ ${aligner} From 0187f8c2c036f301005450eaa635c6640ab610e8 Mon Sep 17 00:00:00 2001 From: gennadyFauna <153561808+gennadyFauna@users.noreply.github.com> Date: Tue, 27 Feb 2024 14:16:38 -0800 Subject: [PATCH 065/111] features too --- modules/local/mtx_to_h5ad.nf | 2 +- modules/local/mtx_to_seurat.nf | 2 +- 2 files changed, 2 insertions(+), 2 deletions(-) diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 7bde6e79..c991b695 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -60,7 +60,7 @@ process MTX_TO_H5AD { --sample ${meta.id} \\ --input *count/counts_unfiltered/cells_x_genes.\${input_type}.mtx \\ --barcode $barcodes_tsv \\ - --feature *count/counts_unfiltered/cells_x_genes.\${input_type}.genes.names.txt \\ + --feature $features_tsv \\ --txp2gene ${txp2gene} \\ --star_index ${star_index} \\ --out ${meta.id}/${meta.id}_\${input_type}_matrix.h5ad ; diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index ac21e639..82ee63cd 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -47,7 +47,7 @@ process MTX_TO_SEURAT { mtx_to_seurat.R \\ *count/counts_unfiltered/cells_x_genes.\${input_type}.mtx \\ $barcodes \\ - *count/counts_unfiltered/cells_x_genes.\${input_type}.genes.names.txt \\ + $features \\ ${meta.id}/${meta.id}_\${input_type}_matrix.rds \\ ${aligner} done From a67a718482463481af6828e15b23290cf6bd6a3f Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 29 Feb 2024 16:12:47 +0000 Subject: [PATCH 066/111] Template update for nf-core/tools version 2.13.1 --- .devcontainer/devcontainer.json | 10 +---- .github/CONTRIBUTING.md | 14 ++++--- .github/PULL_REQUEST_TEMPLATE.md | 2 +- .github/workflows/awsfulltest.yml | 4 +- .github/workflows/awstest.yml | 4 +- .github/workflows/ci.yml | 2 +- .github/workflows/download_pipeline.yml | 2 +- .github/workflows/linting.yml | 2 +- .github/workflows/release-announcements.yml | 2 +- .gitpod.yml | 6 +-- README.md | 3 +- modules.json | 8 ++-- modules/nf-core/multiqc/environment.yml | 2 +- modules/nf-core/multiqc/main.nf | 4 +- .../nf-core/multiqc/tests/main.nf.test.snap | 12 +++--- .../utils_nfcore_scrnaseq_pipeline/main.nf | 10 +++-- .../tests/main.function.nf.test | 2 +- .../tests/main.function.nf.test.snap | 12 +++++- .../tests/main.workflow.nf.test | 20 ++------- .../tests/nextflow.config | 2 +- .../tests/main.function.nf.test.snap | 42 +++++++++++++++---- .../tests/main.workflow.nf.test.snap | 6 ++- .../tests/main.nf.test | 2 +- 23 files changed, 99 insertions(+), 74 deletions(-) diff --git a/.devcontainer/devcontainer.json b/.devcontainer/devcontainer.json index 4ecfbfe3..b290e090 100644 --- a/.devcontainer/devcontainer.json +++ b/.devcontainer/devcontainer.json @@ -10,15 +10,7 @@ "vscode": { // Set *default* container specific settings.json values on container create. "settings": { - "python.defaultInterpreterPath": "/opt/conda/bin/python", - "python.linting.enabled": true, - "python.linting.pylintEnabled": true, - "python.formatting.autopep8Path": "/opt/conda/bin/autopep8", - "python.formatting.yapfPath": "/opt/conda/bin/yapf", - "python.linting.flake8Path": "/opt/conda/bin/flake8", - "python.linting.pycodestylePath": "/opt/conda/bin/pycodestyle", - "python.linting.pydocstylePath": "/opt/conda/bin/pydocstyle", - "python.linting.pylintPath": "/opt/conda/bin/pylint" + "python.defaultInterpreterPath": "/opt/conda/bin/python" }, // Add the IDs of extensions you want installed when the container is created. diff --git a/.github/CONTRIBUTING.md b/.github/CONTRIBUTING.md index 597c854d..d2dc65c3 100644 --- a/.github/CONTRIBUTING.md +++ b/.github/CONTRIBUTING.md @@ -9,9 +9,8 @@ Please use the pre-filled template to save time. However, don't be put off by this template - other more general issues and suggestions are welcome! Contributions to the code are even more welcome ;) -:::info -If you need help using or modifying nf-core/scrnaseq then the best place to ask is on the nf-core Slack [#scrnaseq](https://nfcore.slack.com/channels/scrnaseq) channel ([join our Slack here](https://nf-co.re/join/slack)). -::: +> [!NOTE] +> If you need help using or modifying nf-core/scrnaseq then the best place to ask is on the nf-core Slack [#scrnaseq](https://nfcore.slack.com/channels/scrnaseq) channel ([join our Slack here](https://nf-co.re/join/slack)). ## Contribution workflow @@ -27,8 +26,11 @@ If you're not used to this workflow with git, you can start with some [docs from ## Tests -You can optionally test your changes by running the pipeline locally. Then it is recommended to use the `debug` profile to -receive warnings about process selectors and other debug info. Example: `nextflow run . -profile debug,test,docker --outdir `. +You have the option to test your changes locally by running the pipeline. For receiving warnings about process selectors and other `debug` information, it is recommended to use the debug profile. Execute all the tests with the following command: + +```bash +nf-test test --profile debug,test,docker --verbose +``` When you create a pull request with changes, [GitHub Actions](https://github.com/features/actions) will run automatic tests. Typically, pull-requests are only fully reviewed when these tests are passing, though of course we can help out before then. @@ -90,7 +92,7 @@ Once there, use `nf-core schema build` to add to `nextflow_schema.json`. Sensible defaults for process resource requirements (CPUs / memory / time) for a process should be defined in `conf/base.config`. These should generally be specified generic with `withLabel:` selectors so they can be shared across multiple processes/steps of the pipeline. A nf-core standard set of labels that should be followed where possible can be seen in the [nf-core pipeline template](https://github.com/nf-core/tools/blob/master/nf_core/pipeline-template/conf/base.config), which has the default process as a single core-process, and then different levels of multi-core configurations for increasingly large memory requirements defined with standardised labels. -The process resources can be passed on to the tool dynamically within the process with the `${task.cpu}` and `${task.memory}` variables in the `script:` block. +The process resources can be passed on to the tool dynamically within the process with the `${task.cpus}` and `${task.memory}` variables in the `script:` block. ### Naming schemes diff --git a/.github/PULL_REQUEST_TEMPLATE.md b/.github/PULL_REQUEST_TEMPLATE.md index 073b2953..0767493c 100644 --- a/.github/PULL_REQUEST_TEMPLATE.md +++ b/.github/PULL_REQUEST_TEMPLATE.md @@ -18,7 +18,7 @@ Learn more about contributing: [CONTRIBUTING.md](https://github.com/nf-core/scrn - [ ] If you've added a new tool - have you followed the pipeline conventions in the [contribution docs](https://github.com/nf-core/scrnaseq/tree/master/.github/CONTRIBUTING.md) - [ ] If necessary, also make a PR on the nf-core/scrnaseq _branch_ on the [nf-core/test-datasets](https://github.com/nf-core/test-datasets) repository. - [ ] Make sure your code lints (`nf-core lint`). -- [ ] Ensure the test suite passes (`nextflow run . -profile test,docker --outdir `). +- [ ] Ensure the test suite passes (`nf-test test main.nf.test -profile test,docker`). - [ ] Check for unexpected warnings in debug mode (`nextflow run . -profile debug,test,docker --outdir `). - [ ] Usage Documentation in `docs/usage.md` is updated. - [ ] Output Documentation in `docs/output.md` is updated. diff --git a/.github/workflows/awsfulltest.yml b/.github/workflows/awsfulltest.yml index fd263945..33ee6733 100644 --- a/.github/workflows/awsfulltest.yml +++ b/.github/workflows/awsfulltest.yml @@ -14,7 +14,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 # TODO nf-core: You can customise AWS full pipeline tests as required # Add full size test data (but still relatively small datasets for few samples) # on the `test_full.config` test runs with only one set of parameters @@ -31,7 +31,7 @@ jobs: } profiles: test_full - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/awstest.yml b/.github/workflows/awstest.yml index 1f58192f..edc7b3a3 100644 --- a/.github/workflows/awstest.yml +++ b/.github/workflows/awstest.yml @@ -12,7 +12,7 @@ jobs: steps: # Launch workflow using Tower CLI tool action - name: Launch workflow via tower - uses: seqeralabs/action-tower-launch@922e5c8d5ac4e918107ec311d2ebbd65e5982b3d # v2 + uses: seqeralabs/action-tower-launch@v2 with: workspace_id: ${{ secrets.TOWER_WORKSPACE_ID }} access_token: ${{ secrets.TOWER_ACCESS_TOKEN }} @@ -25,7 +25,7 @@ jobs: } profiles: test - - uses: actions/upload-artifact@5d5d22a31266ced268874388b861e4b58bb5c2f3 # v4 + - uses: actions/upload-artifact@v4 with: name: Tower debug log file path: | diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index e2c989ca..7b27c964 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -31,7 +31,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 with: version: "${{ matrix.NXF_VER }}" diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index f823210d..08622fd5 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -28,7 +28,7 @@ jobs: runs-on: ubuntu-latest steps: - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/linting.yml b/.github/workflows/linting.yml index 748b4311..073e1876 100644 --- a/.github/workflows/linting.yml +++ b/.github/workflows/linting.yml @@ -35,7 +35,7 @@ jobs: uses: actions/checkout@b4ffde65f46336ab88eb53be808477a3936bae11 # v4 - name: Install Nextflow - uses: nf-core/setup-nextflow@b9f764e8ba5c76b712ace14ecbfcef0e40ae2dd8 # v1 + uses: nf-core/setup-nextflow@v1 - uses: actions/setup-python@0a5c61591373683505ea898e09a3ea4f39ef2b9c # v5 with: diff --git a/.github/workflows/release-announcements.yml b/.github/workflows/release-announcements.yml index c3674af2..d468aeaa 100644 --- a/.github/workflows/release-announcements.yml +++ b/.github/workflows/release-announcements.yml @@ -12,7 +12,7 @@ jobs: - name: get topics and convert to hashtags id: get_topics run: | - curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' > $GITHUB_OUTPUT + curl -s https://nf-co.re/pipelines.json | jq -r '.remote_workflows[] | select(.full_name == "${{ github.repository }}") | .topics[]' | awk '{print "#"$0}' | tr '\n' ' ' >> $GITHUB_OUTPUT - uses: rzr/fediverse-action@master with: diff --git a/.gitpod.yml b/.gitpod.yml index 363d5b1d..105a1821 100644 --- a/.gitpod.yml +++ b/.gitpod.yml @@ -10,13 +10,11 @@ tasks: vscode: extensions: # based on nf-core.nf-core-extensionpack - - codezombiech.gitignore # Language support for .gitignore files - # - cssho.vscode-svgviewer # SVG viewer - esbenp.prettier-vscode # Markdown/CommonMark linting and style checking for Visual Studio Code - - eamodio.gitlens # Quickly glimpse into whom, why, and when a line or code block was changed - EditorConfig.EditorConfig # override user/workspace settings with settings found in .editorconfig files - Gruntfuggly.todo-tree # Display TODO and FIXME in a tree view in the activity bar - mechatroner.rainbow-csv # Highlight columns in csv files in different colors - # - nextflow.nextflow # Nextflow syntax highlighting + # - nextflow.nextflow # Nextflow syntax highlighting - oderwat.indent-rainbow # Highlight indentation level - streetsidesoftware.code-spell-checker # Spelling checker for source code + - charliermarsh.ruff # Code linter Ruff diff --git a/README.md b/README.md index c80f96b1..d7e24902 100644 --- a/README.md +++ b/README.md @@ -7,12 +7,13 @@ [![GitHub Actions CI Status](https://github.com/nf-core/scrnaseq/actions/workflows/ci.yml/badge.svg)](https://github.com/nf-core/scrnaseq/actions/workflows/ci.yml) [![GitHub Actions Linting Status](https://github.com/nf-core/scrnaseq/actions/workflows/linting.yml/badge.svg)](https://github.com/nf-core/scrnaseq/actions/workflows/linting.yml)[![AWS CI](https://img.shields.io/badge/CI%20tests-full%20size-FF9900?labelColor=000000&logo=Amazon%20AWS)](https://nf-co.re/scrnaseq/results)[![Cite with Zenodo](http://img.shields.io/badge/DOI-10.5281/zenodo.XXXXXXX-1073c8?labelColor=000000)](https://doi.org/10.5281/zenodo.XXXXXXX) +[![nf-test](https://img.shields.io/badge/unit_tests-nf--test-337ab7.svg)](https://www.nf-test.com) [![Nextflow](https://img.shields.io/badge/nextflow%20DSL2-%E2%89%A523.04.0-23aa62.svg)](https://www.nextflow.io/) [![run with conda](http://img.shields.io/badge/run%20with-conda-3EB049?labelColor=000000&logo=anaconda)](https://docs.conda.io/en/latest/) [![run with docker](https://img.shields.io/badge/run%20with-docker-0db7ed?labelColor=000000&logo=docker)](https://www.docker.com/) [![run with singularity](https://img.shields.io/badge/run%20with-singularity-1d355c.svg?labelColor=000000)](https://sylabs.io/docs/) -[![Launch on Nextflow Tower](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Nextflow%20Tower-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/scrnaseq) +[![Launch on Seqera Platform](https://img.shields.io/badge/Launch%20%F0%9F%9A%80-Seqera%20Platform-%234256e7)](https://tower.nf/launch?pipeline=https://github.com/nf-core/scrnaseq) [![Get help on Slack](http://img.shields.io/badge/slack-nf--core%20%23scrnaseq-4A154B?labelColor=000000&logo=slack)](https://nfcore.slack.com/channels/scrnaseq)[![Follow on Twitter](http://img.shields.io/badge/twitter-%40nf__core-1DA1F2?labelColor=000000&logo=twitter)](https://twitter.com/nf_core)[![Follow on Mastodon](https://img.shields.io/badge/mastodon-nf__core-6364ff?labelColor=FFFFFF&logo=mastodon)](https://mstdn.science/@nf_core)[![Watch on YouTube](http://img.shields.io/badge/youtube-nf--core-FF0000?labelColor=000000&logo=youtube)](https://www.youtube.com/c/nf-core) diff --git a/modules.json b/modules.json index 59464672..01d274c2 100644 --- a/modules.json +++ b/modules.json @@ -12,7 +12,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "ccacf6f5de6df3bc6d73b665c1fd2933d8bbc290", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] } } @@ -21,17 +21,17 @@ "nf-core": { "utils_nextflow_pipeline": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", - "git_sha": "262b17ed2aad591039f914951659177e6c39a8d8", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", - "git_sha": "cd08c91373cd00a73255081340e4914485846ba1", + "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", "installed_by": ["subworkflows"] } } diff --git a/modules/nf-core/multiqc/environment.yml b/modules/nf-core/multiqc/environment.yml index 2212096a..ca39fb67 100644 --- a/modules/nf-core/multiqc/environment.yml +++ b/modules/nf-core/multiqc/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::multiqc=1.20 + - bioconda::multiqc=1.21 diff --git a/modules/nf-core/multiqc/main.nf b/modules/nf-core/multiqc/main.nf index 354f4430..47ac352f 100644 --- a/modules/nf-core/multiqc/main.nf +++ b/modules/nf-core/multiqc/main.nf @@ -3,8 +3,8 @@ process MULTIQC { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/multiqc:1.20--pyhdfd78af_0' : - 'biocontainers/multiqc:1.20--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/multiqc:1.21--pyhdfd78af_0' : + 'biocontainers/multiqc:1.21--pyhdfd78af_0' }" input: path multiqc_files, stageAs: "?/*" diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index c204b488..bfebd802 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:28:51.744211298" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:28.847433492" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,d320d4c37e349c5588e07e7a31cd4186" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T09:29:13.223621555" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file diff --git a/subworkflows/local/utils_nfcore_scrnaseq_pipeline/main.nf b/subworkflows/local/utils_nfcore_scrnaseq_pipeline/main.nf index dc90ad90..841a0a43 100644 --- a/subworkflows/local/utils_nfcore_scrnaseq_pipeline/main.nf +++ b/subworkflows/local/utils_nfcore_scrnaseq_pipeline/main.nf @@ -1,5 +1,5 @@ // -// Subworkflow with functionality specific to the nf-core/pipeline pipeline +// Subworkflow with functionality specific to the nf-core/scrnaseq pipeline // /* @@ -152,7 +152,9 @@ workflow PIPELINE_COMPLETION { // def validateInputParameters() { genomeExistsError() -}// +} + +// // Validate channels from input samplesheet // def validateInputSamplesheet(input) { @@ -190,7 +192,9 @@ def genomeExistsError() { "~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~" error(error_string) } -}// +} + +// // Generate methods description for MultiQC // def toolCitationText() { diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test index 8ed4310c..68718e4f 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test @@ -51,4 +51,4 @@ nextflow_function { ) } } -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap index db2030f8..e3f0baf4 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.function.nf.test.snap @@ -3,10 +3,18 @@ "content": [ "v9.9.9" ], - "timestamp": "2024-01-19T11:32:36.031083" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:05.308243" }, "Test Function checkCondaChannels": { "content": null, - "timestamp": "2024-01-19T11:32:50.456" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:12.425833" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test index f7c54bc6..ca964ce8 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/main.workflow.nf.test @@ -11,9 +11,6 @@ nextflow_workflow { test("Should run no inputs") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = false @@ -39,9 +36,6 @@ nextflow_workflow { test("Should print version") { when { - params { - outdir = "tests/results" - } workflow { """ print_version = true @@ -68,19 +62,16 @@ nextflow_workflow { test("Should dump params") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = 'results' check_conda_channels = false input[0] = false input[1] = true - input[2] = params.outdir + input[2] = outdir input[3] = false """ } @@ -96,19 +87,16 @@ nextflow_workflow { test("Should not create params JSON if no output directory") { when { - params { - outdir = "$outputDir" - } workflow { """ print_version = false dump_parameters = true - outdir = params.outdir + outdir = null check_conda_channels = false input[0] = false input[1] = true - input[2] = null + input[2] = outdir input[3] = false """ } diff --git a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config index 53574ffe..d0a926bf 100644 --- a/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config +++ b/subworkflows/nf-core/utils_nextflow_pipeline/tests/nextflow.config @@ -6,4 +6,4 @@ manifest { nextflowVersion = '!>=23.04.0' version = '9.9.9' doi = 'https://doi.org/10.5281/zenodo.5070524' -} \ No newline at end of file +} diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap index 10f948e6..1037232c 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.function.nf.test.snap @@ -1,25 +1,41 @@ { "Test Function checkProfileProvided": { "content": null, - "timestamp": "2024-02-09T15:43:55.145717" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:03.360873" }, "Test Function checkConfigProvided": { "content": [ true ], - "timestamp": "2024-01-19T11:34:13.548431224" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:02:59.729647" }, "Test Function nfCoreLogo": { "content": [ "\n\n-\u001b[2m----------------------------------------------------\u001b[0m-\n \u001b[0;32m,--.\u001b[0;30m/\u001b[0;32m,-.\u001b[0m\n\u001b[0;34m ___ __ __ __ ___ \u001b[0;32m/,-._.--~'\u001b[0m\n\u001b[0;34m |\\ | |__ __ / ` / \\ |__) |__ \u001b[0;33m} {\u001b[0m\n\u001b[0;34m | \\| | \\__, \\__/ | \\ |___ \u001b[0;32m\\`-._,-`-,\u001b[0m\n \u001b[0;32m`._,._,'\u001b[0m\n\u001b[0;35m nextflow_workflow v9.9.9\u001b[0m\n-\u001b[2m----------------------------------------------------\u001b[0m-\n" ], - "timestamp": "2024-01-19T11:34:38.840454873" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:10.562934" }, "Test Function workflowCitation": { "content": [ "If you use nextflow_workflow for your analysis please cite:\n\n* The pipeline\n https://doi.org/10.5281/zenodo.5070524\n\n* The nf-core framework\n https://doi.org/10.1038/s41587-020-0439-x\n\n* Software dependencies\n https://github.com/nextflow_workflow/blob/master/CITATIONS.md" ], - "timestamp": "2024-01-19T11:34:22.24352016" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:07.019761" }, "Test Function without logColours": { "content": [ @@ -73,13 +89,21 @@ "biwhite": "" } ], - "timestamp": "2024-01-19T11:35:04.418416984" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:17.969323" }, "Test Function dashedLine": { "content": [ "-\u001b[2m----------------------------------------------------\u001b[0m-" ], - "timestamp": "2024-01-19T11:34:55.420000755" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:14.366181" }, "Test Function with logColours": { "content": [ @@ -133,6 +157,10 @@ "biwhite": "\u001b[1;97m" } ], - "timestamp": "2024-01-19T11:35:13.436366565" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:21.714424" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap index d07ce54c..859d1030 100644 --- a/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap +++ b/subworkflows/nf-core/utils_nfcore_pipeline/tests/main.workflow.nf.test.snap @@ -10,6 +10,10 @@ ] } ], - "timestamp": "2024-01-19T11:35:22.538940073" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-28T12:03:25.726491" } } \ No newline at end of file diff --git a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test index 517ee54e..5784a33f 100644 --- a/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test +++ b/subworkflows/nf-core/utils_nfvalidation_plugin/tests/main.nf.test @@ -197,4 +197,4 @@ nextflow_workflow { ) } } -} \ No newline at end of file +} From 35dc9737fb68906e1ae664765411c06eb36805d2 Mon Sep 17 00:00:00 2001 From: EC2 Default User Date: Mon, 4 Mar 2024 17:23:34 +0000 Subject: [PATCH 067/111] version bump --- .nf-test.log | 17 +++ modules.json | 70 ++++++--- .../nf-core/kallistobustools/count/main.nf | 18 ++- .../kallistobustools/count/tests/main.nf.test | 98 +++++++++++++ .../count/tests/main.nf.test.snap | 100 +++++++++++++ .../count/tests/nextflow.config | 5 + .../kallistobustools/count/tests/tags.yml | 2 + modules/nf-core/kallistobustools/ref/main.nf | 28 ++++ .../kallistobustools/ref/tests/main.nf.test | 120 ++++++++++++++++ .../ref/tests/main.nf.test.snap | 136 ++++++++++++++++++ .../kallistobustools/ref/tests/tags.yml | 2 + tests/.nf-test.log | 21 +++ 12 files changed, 595 insertions(+), 22 deletions(-) create mode 100644 .nf-test.log create mode 100644 modules/nf-core/kallistobustools/count/tests/main.nf.test create mode 100644 modules/nf-core/kallistobustools/count/tests/main.nf.test.snap create mode 100644 modules/nf-core/kallistobustools/count/tests/nextflow.config create mode 100644 modules/nf-core/kallistobustools/count/tests/tags.yml create mode 100644 modules/nf-core/kallistobustools/ref/tests/main.nf.test create mode 100644 modules/nf-core/kallistobustools/ref/tests/main.nf.test.snap create mode 100644 modules/nf-core/kallistobustools/ref/tests/tags.yml create mode 100644 tests/.nf-test.log diff --git a/.nf-test.log b/.nf-test.log new file mode 100644 index 00000000..ca137888 --- /dev/null +++ b/.nf-test.log @@ -0,0 +1,17 @@ +Feb-27 21:54:16.881 [main] INFO com.askimed.nf.test.App - nf-test 0.8.4 +Feb-27 21:54:16.895 [main] INFO com.askimed.nf.test.App - Arguments: [test, tests/main_pipeline_kallisto.test, --update-snapshot] +Feb-27 21:54:17.590 [main] INFO com.askimed.nf.test.App - Nextflow Version: 23.10.1 +Feb-27 21:54:17.592 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Load config from file /home/ec2-user/scrnaseq/nf-test.config... +Feb-27 21:54:17.887 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Detected 1 test files. +Feb-27 21:54:18.162 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/home/ec2-user/scrnaseq/bin' to '/home/ec2-user/scrnaseq/.nf-test/tests/72e0d0f6b834ab79755c0f97ef8c4ca8/meta/bin' +Feb-27 21:54:18.164 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/home/ec2-user/scrnaseq/lib' to '/home/ec2-user/scrnaseq/.nf-test/tests/72e0d0f6b834ab79755c0f97ef8c4ca8/meta/lib' +Feb-27 21:54:18.164 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/home/ec2-user/scrnaseq/assets' to '/home/ec2-user/scrnaseq/.nf-test/tests/72e0d0f6b834ab79755c0f97ef8c4ca8/meta/assets' +Feb-27 21:54:18.171 [main] DEBUG com.askimed.nf.test.core.AbstractTest - Stage 0 user provided files... +Feb-27 21:54:18.174 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Started test plan +Feb-27 21:54:18.174 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Running testsuite 'Test Workflow main.nf' from file '/home/ec2-user/scrnaseq/tests/main_pipeline_kallisto.test'. +Feb-27 21:54:18.174 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Run test '72e0d0f6: test-dataset_kallisto_aligner'. type: com.askimed.nf.test.lang.pipeline.PipelineTest +Feb-27 21:55:36.281 [main] DEBUG com.askimed.nf.test.lang.extensions.SnapshotFile - Load snapshots from file '/home/ec2-user/scrnaseq/tests/main_pipeline_kallisto.test.snap' +Feb-27 21:55:36.305 [main] DEBUG com.askimed.nf.test.lang.extensions.Snapshot - Snapshots 'test-dataset_kallisto_aligner' match. +Feb-27 21:55:36.306 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Test '72e0d0f6: test-dataset_kallisto_aligner' finished. status: PASSED +Feb-27 21:55:36.308 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Testsuite 'Test Workflow main.nf' finished. snapshot file: true, skipped tests: false, failed tests: false +Feb-27 21:55:36.308 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Executed 1 tests. 0 tests failed. Done! diff --git a/modules.json b/modules.json index c71a0499..51864fc4 100644 --- a/modules.json +++ b/modules.json @@ -8,85 +8,117 @@ "cellranger/count": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellranger/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellranger/mkref": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellrangerarc/count": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellrangerarc/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellrangerarc/mkref": { "branch": "master", "git_sha": "4196b1b2e7ce265892f3979eabf7a9ddc030702f", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gffread": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gunzip": { "branch": "master", "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "kallistobustools/count": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", + "installed_by": [ + "modules" + ] }, "kallistobustools/ref": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "9e71d8519dfbfc328c078bba14d4bd4c99e39a94", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "star/genomegenerate": { "branch": "master", "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "universc": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "unzip": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index 67ad5812..841ea2fe 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -17,9 +17,9 @@ process KALLISTOBUSTOOLS_COUNT { val workflow_mode output: - tuple val(meta), path ("*.count"), emit: count - path "versions.yml" , emit: versions - path "*.count/*/*.mtx" , emit: matrix //Ensure that kallisto finished and produced outputs + tuple val(meta), path ("*.count") , emit: count + path "versions.yml" , emit: versions + path "*.count/*/*.mtx" , emit: matrix //Ensure that kallisto finished and produced outputs when: task.ext.when == null || task.ext.when @@ -50,4 +50,16 @@ process KALLISTOBUSTOOLS_COUNT { kallistobustools: \$(echo \$(kb --version 2>&1) | sed 's/^.*kb_python //;s/positional arguments.*\$//') END_VERSIONS """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + mkdir -p ${prefix}.count/counts_unfiltered/ + touch ${prefix}.count/counts_unfiltered/cells_x_genes.mtx + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kallistobustools: \$(echo \$(kb --version 2>&1) | sed 's/^.*kb_python //;s/positional arguments.*\$//') + END_VERSIONS + """ } diff --git a/modules/nf-core/kallistobustools/count/tests/main.nf.test b/modules/nf-core/kallistobustools/count/tests/main.nf.test new file mode 100644 index 00000000..550001f9 --- /dev/null +++ b/modules/nf-core/kallistobustools/count/tests/main.nf.test @@ -0,0 +1,98 @@ +nextflow_process { + + name "Test Process KALLISTOBUSTOOLS_COUNT" + script "../main.nf" + process "KALLISTOBUSTOOLS_COUNT" + + tag "modules" + tag "modules_nfcore" + tag "kallistobustools" + tag "kallistobustools/count" + tag "kallistobustools/ref" + + setup { + run("KALLISTOBUSTOOLS_REF") { + script "../../ref/main.nf" + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "standard" + """ + } + } + } + + test("genome.fasta + genome.gtf + '10X3' + 'standard'") { + + when { + process { + """ + input[0] = Channel.of( + [ + [id:'test'], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R1_001.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R2_001.fastq.gz', checkIfExists: true) + ] + ] + ) + input[1] = KALLISTOBUSTOOLS_REF.out.index + input[2] = KALLISTOBUSTOOLS_REF.out.t2g + input[3] = KALLISTOBUSTOOLS_REF.out.cdna_t2c.ifEmpty{ [] } // when empty the module doesn't run unless something is passed. + input[4] = KALLISTOBUSTOOLS_REF.out.intron_t2c.ifEmpty{ [] } // when empty the module doesn't run unless something is passed. + input[5] = "10XV3" + input[6] = "standard" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + process.out.matrix, + path(process.out.count.get(0).get(1)).list().findAll { file(it.toString()).name != "run_info.json" && file(it.toString()).name != "kb_info.json" }, + file(path(process.out.count.get(0).get(1)).list().find { file(it.toString()).name == "kb_info.json" }.toString()).readLines()[15..22], + file(path(process.out.count.get(0).get(1)).list().find { file(it.toString()).name == "run_info.json" }.toString()).readLines()[0..9] + ).match() + } + ) + } + } + + test("genome.fasta + genome.gtf + '10X3' + 'standard' - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of( + [ + [id:'test'], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R1_001.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R2_001.fastq.gz', checkIfExists: true) + ] + ] + ) + input[1] = KALLISTOBUSTOOLS_REF.out.index + input[2] = KALLISTOBUSTOOLS_REF.out.t2g + input[3] = KALLISTOBUSTOOLS_REF.out.cdna_t2c.ifEmpty{ [] } // when empty the module doesn't run unless something is passed. + input[4] = KALLISTOBUSTOOLS_REF.out.intron_t2c.ifEmpty{ [] } // when empty the module doesn't run unless something is passed. + input[5] = "10XV3" + input[6] = "standard" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap b/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap new file mode 100644 index 00000000..3378c3c1 --- /dev/null +++ b/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap @@ -0,0 +1,100 @@ +{ + "genome.fasta + genome.gtf + '10X3' + 'standard' - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + [ + [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ] + ], + "1": [ + "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" + ], + "2": [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "count": [ + [ + { + "id": "test" + }, + [ + [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ] + ], + "matrix": [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "versions": [ + "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-01T15:48:45.775904562" + }, + "genome.fasta + genome.gtf + '10X3' + 'standard'": { + "content": [ + [ + "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" + ], + [ + "cells_x_genes.mtx:md5,e12a45e7f7d6527f698dd9cb2e99ecd1" + ], + [ + "10x_version3_whitelist.txt:md5,3d36d0a4021fd292b265e2b5e72aaaf3", + [ + "cells_x_genes.barcodes.txt:md5,41f7adaf43b60f2f4f62d6a7073688de", + "cells_x_genes.genes.names.txt:md5,b29afa75be300c7f24fbd0740a66689b", + "cells_x_genes.genes.txt:md5,fe6d5501923867b514a0447aa4b4995f", + "cells_x_genes.mtx:md5,e12a45e7f7d6527f698dd9cb2e99ecd1" + ], + "inspect.json:md5,bafb47a58ac1bbf9be953f21c361d266", + "matrix.ec:md5,31a4c1a3e8e0c562b12f6569ffbf5459", + "output.bus:md5,d6fa0612a4a16eaf8a3e08bdc13ff49c", + "output.unfiltered.bus:md5,bf899b967657f612ba864188868d58cc", + "transcripts.txt:md5,23861cf43033e7c596e6989a88a3a373" + ], + [ + " \"commands\": [", + " \"kallisto bus -i kb_ref_out.idx -o test.count -x 10XV3 -t 2 subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R1_001.fastq.gz subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R2_001.fastq.gz\",", + " \"bustools sort -o test.count/tmp/output.s.bus -T test.count/tmp -t 2 -m 2G test.count/output.bus\",", + " \"bustools inspect -o test.count/inspect.json -w test.count/10x_version3_whitelist.txt test.count/tmp/output.s.bus\",", + " \"bustools correct -o test.count/tmp/output.s.c.bus -w test.count/10x_version3_whitelist.txt test.count/tmp/output.s.bus\",", + " \"bustools sort -o test.count/output.unfiltered.bus -T test.count/tmp -t 2 -m 2G test.count/tmp/output.s.c.bus\",", + " \"bustools count -o test.count/counts_unfiltered/cells_x_genes -g t2g.txt -e test.count/matrix.ec -t test.count/transcripts.txt --genecounts --umi-gene test.count/output.unfiltered.bus\"", + " ]," + ], + [ + "{", + "\t\"n_targets\": 12,", + "\t\"n_bootstraps\": 0,", + "\t\"n_processed\": 10000,", + "\t\"n_pseudoaligned\": 26,", + "\t\"n_unique\": 26,", + "\t\"p_pseudoaligned\": 0.3,", + "\t\"p_unique\": 0.3,", + "\t\"kallisto_version\": \"0.50.1\",", + "\t\"index_version\": 13," + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-01T15:48:24.476953631" + } +} \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/count/tests/nextflow.config b/modules/nf-core/kallistobustools/count/tests/nextflow.config new file mode 100644 index 00000000..7a5cbfb4 --- /dev/null +++ b/modules/nf-core/kallistobustools/count/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: KALLISTOBUSTOOLS_COUNT { + ext.args = '--cellranger -m 1' + } +} diff --git a/modules/nf-core/kallistobustools/count/tests/tags.yml b/modules/nf-core/kallistobustools/count/tests/tags.yml new file mode 100644 index 00000000..9c432071 --- /dev/null +++ b/modules/nf-core/kallistobustools/count/tests/tags.yml @@ -0,0 +1,2 @@ +kallistobustools/count: + - "modules/nf-core/kallistobustools/count/**" diff --git a/modules/nf-core/kallistobustools/ref/main.nf b/modules/nf-core/kallistobustools/ref/main.nf index 702317d3..0b45203d 100644 --- a/modules/nf-core/kallistobustools/ref/main.nf +++ b/modules/nf-core/kallistobustools/ref/main.nf @@ -62,4 +62,32 @@ process KALLISTOBUSTOOLS_REF { END_VERSIONS """ } + + stub: + if (workflow_mode == "standard") { + """ + touch kb_ref_out.idx \\ + touch t2g.txt \\ + touch cdna.fa + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kallistobustools: \$(echo \$(kb --version 2>&1) | sed 's/^.*kb_python //;s/positional arguments.*\$//') + END_VERSIONS + """ + } else { + """ + touch kb_ref_out.idx \\ + touch t2g.txt \\ + touch cdna.fa + touch intron.fa \\ + touch cdna_t2c.txt \\ + touch intron_t2c.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kallistobustools: \$(echo \$(kb --version 2>&1) | sed 's/^.*kb_python //;s/positional arguments.*\$//') + END_VERSIONS + """ + } } diff --git a/modules/nf-core/kallistobustools/ref/tests/main.nf.test b/modules/nf-core/kallistobustools/ref/tests/main.nf.test new file mode 100644 index 00000000..dc49d9ac --- /dev/null +++ b/modules/nf-core/kallistobustools/ref/tests/main.nf.test @@ -0,0 +1,120 @@ +nextflow_process { + + name "Test Process KALLISTOBUSTOOLS_REF" + script "../main.nf" + process "KALLISTOBUSTOOLS_REF" + + tag "modules" + tag "modules_nfcore" + tag "kallistobustools" + tag "kallistobustools/ref" + + test("genome.fasta + genome.gtf + 'standard'") { + + when { + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "standard" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + process.out.t2g, + process.out.cdna, + process.out.intron, + process.out.cdna_t2c, + process.out.intron_t2c, + ).match() + }, + { assert file(process.out.index.get(0)).exists() } + ) + } + } + + test("genome.fasta + genome.gtf + 'nac'") { + + when { + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "nac" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + process.out.t2g, + process.out.cdna, + process.out.intron, + process.out.cdna_t2c, + process.out.intron_t2c, + ).match() + }, + { assert file(process.out.index.get(0)).exists() } + ) + } + } + + test("genome.fasta + genome.gtf + 'standard' - stub") { + + options "-stub" + + when { + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "standard" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("genome.fasta + genome.gtf + 'nac' - stub") { + + when { + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "nac" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + process.out.t2g, + process.out.cdna, + process.out.intron, + process.out.cdna_t2c, + process.out.intron_t2c, + ).match() + }, + { assert file(process.out.index.get(0)).exists() } + ) + } + } +} diff --git a/modules/nf-core/kallistobustools/ref/tests/main.nf.test.snap b/modules/nf-core/kallistobustools/ref/tests/main.nf.test.snap new file mode 100644 index 00000000..9c2be8a7 --- /dev/null +++ b/modules/nf-core/kallistobustools/ref/tests/main.nf.test.snap @@ -0,0 +1,136 @@ +{ + "genome.fasta + genome.gtf + 'standard'": { + "content": [ + [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ], + [ + "t2g.txt:md5,d1a8a22c59b9cb0bda39c0c9bb3f6afe" + ], + [ + "cdna.fa:md5,7bca59288fab822451de250d2eee48dc" + ], + [ + + ], + [ + + ], + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T23:37:43.739374859" + }, + "genome.fasta + genome.gtf + 'nac'": { + "content": [ + [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ], + [ + "t2g.txt:md5,58591306b33bb948bac7b40f346d0cd7" + ], + [ + "cdna.fa:md5,7bca59288fab822451de250d2eee48dc" + ], + [ + "intron.fa:md5,1aad4e3f5d006f495cc6647fa0bbf6ff" + ], + [ + "cdna_t2c.txt:md5,23861cf43033e7c596e6989a88a3a373" + ], + [ + "intron_t2c.txt:md5,fe6d5501923867b514a0447aa4b4995f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T23:37:59.436989671" + }, + "genome.fasta + genome.gtf + 'standard' - stub": { + "content": [ + { + "0": [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ], + "1": [ + "kb_ref_out.idx:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "2": [ + "t2g.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "3": [ + "cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "4": [ + + ], + "5": [ + + ], + "6": [ + + ], + "cdna": [ + "cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "cdna_t2c": [ + + ], + "index": [ + "kb_ref_out.idx:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "intron": [ + + ], + "intron_t2c": [ + + ], + "t2g": [ + "t2g.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "versions": [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T23:38:09.578411047" + }, + "genome.fasta + genome.gtf + 'nac' - stub": { + "content": [ + [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ], + [ + "t2g.txt:md5,58591306b33bb948bac7b40f346d0cd7" + ], + [ + "cdna.fa:md5,7bca59288fab822451de250d2eee48dc" + ], + [ + "intron.fa:md5,1aad4e3f5d006f495cc6647fa0bbf6ff" + ], + [ + "cdna_t2c.txt:md5,23861cf43033e7c596e6989a88a3a373" + ], + [ + "intron_t2c.txt:md5,fe6d5501923867b514a0447aa4b4995f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T23:38:25.355912473" + } +} \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/ref/tests/tags.yml b/modules/nf-core/kallistobustools/ref/tests/tags.yml new file mode 100644 index 00000000..208c8d27 --- /dev/null +++ b/modules/nf-core/kallistobustools/ref/tests/tags.yml @@ -0,0 +1,2 @@ +kallistobustools/ref: + - "modules/nf-core/kallistobustools/ref/**" diff --git a/tests/.nf-test.log b/tests/.nf-test.log new file mode 100644 index 00000000..8251fc75 --- /dev/null +++ b/tests/.nf-test.log @@ -0,0 +1,21 @@ +Feb-27 21:54:09.971 [main] INFO com.askimed.nf.test.App - nf-test 0.8.4 +Feb-27 21:54:09.988 [main] INFO com.askimed.nf.test.App - Arguments: [test, tests/main_pipeline_kallisto.test, --update-snapshot] +Feb-27 21:54:10.670 [main] INFO com.askimed.nf.test.App - Nextflow Version: 23.10.1 +Feb-27 21:54:10.674 [main] WARN com.askimed.nf.test.commands.RunTestsCommand - No nf-test config file found. +Feb-27 21:54:10.674 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Detected 1 test files. +Feb-27 21:54:10.676 [main] ERROR com.askimed.nf.test.commands.RunTestsCommand - Running tests failed. +java.lang.Exception: Test file '/home/ec2-user/scrnaseq/tests/tests/main_pipeline_kallisto.test' not found. + at com.askimed.nf.test.core.TestExecutionEngine.parse(TestExecutionEngine.java:116) + at com.askimed.nf.test.core.TestExecutionEngine.execute(TestExecutionEngine.java:159) + at com.askimed.nf.test.commands.RunTestsCommand.execute(RunTestsCommand.java:184) + at com.askimed.nf.test.commands.AbstractCommand.call(AbstractCommand.java:43) + at com.askimed.nf.test.commands.AbstractCommand.call(AbstractCommand.java:18) + at picocli.CommandLine.executeUserObject(CommandLine.java:1953) + at picocli.CommandLine.access$1300(CommandLine.java:145) + at picocli.CommandLine$RunLast.executeUserObjectOfLastSubcommandWithSameParent(CommandLine.java:2352) + at picocli.CommandLine$RunLast.handle(CommandLine.java:2346) + at picocli.CommandLine$RunLast.handle(CommandLine.java:2311) + at picocli.CommandLine$AbstractParseResultHandler.execute(CommandLine.java:2179) + at picocli.CommandLine.execute(CommandLine.java:2078) + at com.askimed.nf.test.App.run(App.java:44) + at com.askimed.nf.test.App.main(App.java:51) From f12cfbbdc89ed0b65737c689f1ef80f7c4e51bdb Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Mon, 4 Mar 2024 19:14:39 +0000 Subject: [PATCH 068/111] [automated] Fix linting with Prettier --- modules.json | 66 ++++++++++++++-------------------------------------- 1 file changed, 17 insertions(+), 49 deletions(-) diff --git a/modules.json b/modules.json index 51864fc4..f6ae83cd 100644 --- a/modules.json +++ b/modules.json @@ -8,117 +8,85 @@ "cellranger/count": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellranger/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellranger/mkref": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/count": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/mkref": { "branch": "master", "git_sha": "4196b1b2e7ce265892f3979eabf7a9ddc030702f", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gffread": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "kallistobustools/count": { "branch": "master", "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "kallistobustools/ref": { "branch": "master", "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "9e71d8519dfbfc328c078bba14d4bd4c99e39a94", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "star/genomegenerate": { "branch": "master", "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "universc": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "unzip": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } } } } -} \ No newline at end of file +} From 5da76822f06416677c2cc1e81b0b0ccb8d984a89 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Wed, 6 Mar 2024 16:34:16 +0100 Subject: [PATCH 069/111] WIP cleanup after tempalte update --- README.md | 4 +- assets/schema_input.json | 28 ++++++- workflows/scrnaseq.nf | 174 +++++++++++++++------------------------ 3 files changed, 92 insertions(+), 114 deletions(-) diff --git a/README.md b/README.md index 23061375..3399f168 100644 --- a/README.md +++ b/README.md @@ -48,8 +48,8 @@ First, prepare a samplesheet with your input data that looks as follows: ```csv sample,fastq_1,fastq_2,expected_cells -pbmc8k,pbmc8k_S1_L007_R1_001.fastq.gz,pbmc8k_S1_L007_R2_001.fastq.gz,"10000" -pbmc8k,pbmc8k_S1_L008_R1_001.fastq.gz,pbmc8k_S1_L008_R2_001.fastq.gz,"10000" +pbmc8k,pbmc8k_S1_L007_R1_001.fastq.gz,pbmc8k_S1_L007_R2_001.fastq.gz,10000 +pbmc8k,pbmc8k_S1_L008_R1_001.fastq.gz,pbmc8k_S1_L008_R2_001.fastq.gz,10000 ``` Each row represents a fastq file (single-end) or a pair of fastq files (paired end). diff --git a/assets/schema_input.json b/assets/schema_input.json index dab0450a..490c54a7 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -11,7 +11,9 @@ "type": "string", "pattern": "^\\S+$", "errorMessage": "Sample name must be provided and cannot contain spaces", - "meta": ["id"] + "meta": [ + "id" + ] }, "fastq_1": { "type": "string", @@ -26,8 +28,28 @@ "exists": true, "pattern": "^\\S+\\.f(ast)?q\\.gz$", "errorMessage": "FastQ file for reads 2 cannot contain spaces and must have extension '.fq.gz' or '.fastq.gz'" + }, + "expected_cells": { + "type": "integer", + "errorMessage": "Expected cells must be an Integer", + "meta": "expected_cells" + }, + "seq_center": { + "type": "string", + "meta": "seq_center" + }, + "sample_type": { + "type": "string", + "enum": [ + "atac", + "gex" + ] } }, - "required": ["sample", "fastq_1"] + "required": [ + "sample", + "fastq_1", + "fastq_2" + ] } -} +} \ No newline at end of file diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 6801d4dc..77cd1d20 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -4,126 +4,87 @@ ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { FASTQC } from '../modules/nf-core/fastqc/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { paramsSummaryMap } from 'plugin/nf-validation' -include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { MULTIQC } from '../modules/nf-core/multiqc/main' + +include { INPUT_CHECK } from '../subworkflows/local/input_check' +include { FASTQC_CHECK } from '../subworkflows/local/fastqc' +include { KALLISTO_BUSTOOLS } from '../subworkflows/local/kallisto_bustools' +include { SCRNASEQ_ALEVIN } from '../subworkflows/local/alevin' +include { STARSOLO } from '../subworkflows/local/starsolo' +include { CELLRANGER_ALIGN } from "../subworkflows/local/align_cellranger" +include { CELLRANGERARC_ALIGN } from "../subworkflows/local/align_cellrangerarc" +include { UNIVERSC_ALIGN } from "../subworkflows/local/align_universc" +include { MTX_CONVERSION } from "../subworkflows/local/mtx_conversion" +include { GTF_GENE_FILTER } from '../modules/local/gtf_gene_filter' + +include { paramsSummaryMap } from 'plugin/nf-validation' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_scrnaseq_pipeline' include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' -def summary_params = paramsSummaryMap(workflow) - -def checkPathParamList = [ - params.input, params.multiqc_config, params.fasta, params.gtf, - params.transcript_fasta, params.salmon_index, params.kallisto_index, - params.star_index, params.txp2gene, params.barcode_whitelist, params.cellranger_index, - params.universc_index -] -for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - CONFIG FILES -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -ch_multiqc_config = Channel.fromPath("$projectDir/assets/multiqc_config.yml", checkIfExists: true) -ch_multiqc_custom_config = params.multiqc_config ? Channel.fromPath( params.multiqc_config, checkIfExists: true ) : Channel.empty() -ch_multiqc_logo = params.multiqc_logo ? Channel.fromPath( params.multiqc_logo, checkIfExists: true ) : Channel.empty() -ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT LOCAL MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// SUBWORKFLOW: Consisting of a mix of local and nf-core/modules -// -include { INPUT_CHECK } from '../subworkflows/local/input_check' -include { FASTQC_CHECK } from '../subworkflows/local/fastqc' -include { KALLISTO_BUSTOOLS } from '../subworkflows/local/kallisto_bustools' -include { SCRNASEQ_ALEVIN } from '../subworkflows/local/alevin' -include { STARSOLO } from '../subworkflows/local/starsolo' -include { CELLRANGER_ALIGN } from "../subworkflows/local/align_cellranger" -include { CELLRANGERARC_ALIGN } from "../subworkflows/local/align_cellrangerarc" -include { UNIVERSC_ALIGN } from "../subworkflows/local/align_universc" -include { MTX_CONVERSION } from "../subworkflows/local/mtx_conversion" -include { GTF_GENE_FILTER } from '../modules/local/gtf_gene_filter' - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT NF-CORE MODULES/SUBWORKFLOWS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// -// MODULE: Installed directly from nf-core/modules -// -include { CUSTOM_DUMPSOFTWAREVERSIONS } from '../modules/nf-core/custom/dumpsoftwareversions/main' -include { MULTIQC } from '../modules/nf-core/multiqc/main' -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - RUN MAIN WORKFLOW -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - -// Info required for completion email and summary -// TODO: Are this channels still necessary? -ch_output_docs = file("$projectDir/docs/output.md", checkIfExists: true) -ch_output_docs_images = file("$projectDir/docs/images/", checkIfExists: true) -protocol_config = WorkflowScrnaseq.getProtocol(workflow, log, params.aligner, params.protocol) -if (protocol_config['protocol'] == 'auto' && params.aligner != "cellranger") { - error "Only cellranger supports `protocol = 'auto'`. Please specify the protocol manually!" -} +workflow SCRNASEQ { -// general input and params -ch_input = file(params.input) -ch_genome_fasta = Channel.value(params.fasta ? file(params.fasta) : []) -ch_gtf = params.gtf ? file(params.gtf) : [] -ch_transcript_fasta = params.transcript_fasta ? file(params.transcript_fasta): [] -ch_motifs = params.motifs ? file(params.motifs) : [] -ch_cellrangerarc_config = params.cellrangerarc_config ? file(params.cellrangerarc_config) : [] -ch_txp2gene = params.txp2gene ? file(params.txp2gene) : [] -ch_multiqc_alevin = Channel.empty() -ch_multiqc_star = Channel.empty() -ch_multiqc_cellranger = Channel.empty() -if (params.barcode_whitelist) { - ch_barcode_whitelist = file(params.barcode_whitelist) -} else if (protocol_config.containsKey("whitelist")) { - ch_barcode_whitelist = file("$projectDir/${protocol_config['whitelist']}") -} else { - ch_barcode_whitelist = [] -} + take: + ch_samplesheet // channel: samplesheet read in from --input + main: -//kallisto params -ch_kallisto_index = params.kallisto_index ? file(params.kallisto_index) : [] -kb_workflow = params.kb_workflow + // TODO: checks still necessary in favor of schema validation? + def checkPathParamList = [ + params.input, params.multiqc_config, params.fasta, params.gtf, + params.transcript_fasta, params.salmon_index, params.kallisto_index, + params.star_index, params.txp2gene, params.barcode_whitelist, params.cellranger_index, + params.universc_index + ] + for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } + + // Info required for completion email and summary + // TODO: Are this channels still necessary? + protocol_config = WorkflowScrnaseq.getProtocol(workflow, log, params.aligner, params.protocol) + if (protocol_config['protocol'] == 'auto' && params.aligner != "cellranger") { + error "Only cellranger supports `protocol = 'auto'`. Please specify the protocol manually!" + } -//salmon params -ch_salmon_index = params.salmon_index ? file(params.salmon_index) : [] + // general input and params + ch_input = file(params.input) + ch_genome_fasta = Channel.value(params.fasta ? file(params.fasta) : []) + ch_gtf = params.gtf ? file(params.gtf) : [] + ch_transcript_fasta = params.transcript_fasta ? file(params.transcript_fasta): [] + ch_motifs = params.motifs ? file(params.motifs) : [] + ch_cellrangerarc_config = params.cellrangerarc_config ? file(params.cellrangerarc_config) : [] + ch_txp2gene = params.txp2gene ? file(params.txp2gene) : [] + ch_multiqc_alevin = Channel.empty() + ch_multiqc_star = Channel.empty() + ch_multiqc_cellranger = Channel.empty() + if (params.barcode_whitelist) { + ch_barcode_whitelist = file(params.barcode_whitelist) + } else if (protocol_config.containsKey("whitelist")) { + ch_barcode_whitelist = file("$projectDir/${protocol_config['whitelist']}") + } else { + ch_barcode_whitelist = [] + } -//star params -ch_star_index = params.star_index ? file(params.star_index) : [] -star_feature = params.star_feature -//cellranger params -ch_cellranger_index = params.cellranger_index ? file(params.cellranger_index) : [] + //kallisto params + ch_kallisto_index = params.kallisto_index ? file(params.kallisto_index) : [] + kb_workflow = params.kb_workflow -//universc params -ch_universc_index = params.universc_index ? file(params.universc_index) : [] + //salmon params + ch_salmon_index = params.salmon_index ? file(params.salmon_index) : [] -workflow SCRNASEQ { + //star params + ch_star_index = params.star_index ? file(params.star_index) : [] + star_feature = params.star_feature - take: - ch_samplesheet // channel: samplesheet read in from --input + //cellranger params + ch_cellranger_index = params.cellranger_index ? file(params.cellranger_index) : [] - main: + //universc params + ch_universc_index = params.universc_index ? file(params.universc_index) : [] ch_versions = Channel.empty() ch_mtx_matrices = Channel.empty() @@ -254,11 +215,6 @@ workflow SCRNASEQ { //Add Versions from MTX Conversion workflow too ch_versions.mix(MTX_CONVERSION.out.ch_versions) - // collect software versions - CUSTOM_DUMPSOFTWAREVERSIONS ( - ch_versions.unique().collectFile(name: 'collated_versions.yml') - ) - // // MODULE: MultiQC // @@ -270,7 +226,7 @@ workflow SCRNASEQ { ch_multiqc_custom_methods_description = params.multiqc_methods_description ? file(params.multiqc_methods_description, checkIfExists: true) : file("$projectDir/assets/methods_description_template.yml", checkIfExists: true) ch_methods_description = Channel.value(methodsDescriptionText(ch_multiqc_custom_methods_description)) ch_multiqc_files = ch_multiqc_files.mix(ch_workflow_summary.collectFile(name: 'workflow_summary_mqc.yaml')) - ch_multiqc_files = ch_multiqc_files.mix(ch_collated_versions) + ch_multiqc_files = ch_multiqc_files.mix(softwareVersionsToYAML(ch_versions).collectFile(name: 'versions.yml')) ch_multiqc_files = ch_multiqc_files.mix(ch_methods_description.collectFile(name: 'methods_description_mqc.yaml', sort: false)) MULTIQC ( From 070f12338df41a4449d1e00ae3fc3c2a8cf1fa5a Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 10:12:43 +0100 Subject: [PATCH 070/111] Move parameter validation to nextflow schema --- assets/schema_input.json | 13 +++++-- lib/WorkflowScrnaseq.groovy | 21 ++++++++++ main.nf | 40 ++++++++++--------- nextflow_schema.json | 74 ++++++++++++++++++++++++++++------- workflows/scrnaseq.nf | 77 +++++++++---------------------------- 5 files changed, 130 insertions(+), 95 deletions(-) create mode 100755 lib/WorkflowScrnaseq.groovy diff --git a/assets/schema_input.json b/assets/schema_input.json index 490c54a7..af6ccfa3 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -32,17 +32,24 @@ "expected_cells": { "type": "integer", "errorMessage": "Expected cells must be an Integer", - "meta": "expected_cells" + "meta": [ + "expected_cells" + ] }, "seq_center": { "type": "string", - "meta": "seq_center" + "meta": [ + "seq_center" + ] }, "sample_type": { "type": "string", "enum": [ "atac", "gex" + ], + "meta": [ + "sample_type" ] } }, @@ -52,4 +59,4 @@ "fastq_2" ] } -} \ No newline at end of file +} diff --git a/lib/WorkflowScrnaseq.groovy b/lib/WorkflowScrnaseq.groovy new file mode 100755 index 00000000..07475fe1 --- /dev/null +++ b/lib/WorkflowScrnaseq.groovy @@ -0,0 +1,21 @@ +import groovy.json.JsonSlurper + + +class WorkflowScrnaseq { + // Retrieve the aligner-specific protocol based on the specified protocol. + // Returns a map ["protocol": protocol, "extra_args": , "whitelist": ] + // extra_args and whitelist are optional. + public static Map getProtocol(workflow, log, aligner, protocol) { + def jsonSlurper = new JsonSlurper() + def json = new File("${workflow.projectDir}/assets/protocols.json").text + def protocols = jsonSlurper.parseText(json) + def aligner_map = protocols[aligner] + if(aligner_map.containsKey(protocol)) { + return aligner_map[protocol] + } else { + log.warn("Protocol '${protocol}' not recognized by the pipeline. Passing on the protocol to the aligner unmodified.") + return ["protocol": protocol] + } + } + +} diff --git a/main.nf b/main.nf index 3471e5b0..4f96908f 100644 --- a/main.nf +++ b/main.nf @@ -82,25 +82,27 @@ workflow { params.input ) - // - // WORKFLOW: Run main workflow - // - NFCORE_SCRNASEQ ( - PIPELINE_INITIALISATION.out.samplesheet - ) - - // - // SUBWORKFLOW: Run completion tasks - // - PIPELINE_COMPLETION ( - params.email, - params.email_on_fail, - params.plaintext_email, - params.outdir, - params.monochrome_logs, - params.hook_url, - NFCORE_SCRNASEQ.out.multiqc_report - ) + PIPELINE_INITIALISATION.out.samplesheet.view() + + // // + // // WORKFLOW: Run main workflow + // // + // NFCORE_SCRNASEQ ( + // PIPELINE_INITIALISATION.out.samplesheet.view() + // ) + + // // + // // SUBWORKFLOW: Run completion tasks + // // + // PIPELINE_COMPLETION ( + // params.email, + // params.email_on_fail, + // params.plaintext_email, + // params.outdir, + // params.monochrome_logs, + // params.hook_url, + // NFCORE_SCRNASEQ.out.multiqc_report + // ) } /* diff --git a/nextflow_schema.json b/nextflow_schema.json index 65b4b695..e9fef834 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,7 +10,10 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": ["input", "outdir"], + "required": [ + "input", + "outdir" + ], "properties": { "input": { "type": "string", @@ -51,7 +54,9 @@ "barcode_whitelist": { "type": "string", "description": "If not using the 10X Genomics platform, a custom barcode whitelist can be used with `--barcode_whitelist`.", - "fa_icon": "fas fa-barcode" + "fa_icon": "fas fa-barcode", + "format": "file-path", + "exists": true }, "aligner": { "type": "string", @@ -59,7 +64,14 @@ "default": "alevin", "help_text": "The workflow can handle three types of methods:\n\n- Kallisto/Bustools\n- Salmon Alevin + AlevinQC\n- STARsolo\n\nTo choose which one to use, please specify either `alevin`, `star` or `kallisto` as a parameter option for `--aligner`. By default, the pipeline runs the `alevin` option. Note that specifying another aligner option also requires choosing appropriate parameters (see below) for the selected option.", "fa_icon": "fas fa-align-center", - "enum": ["kallisto", "star", "alevin", "cellranger", "cellrangerarc", "universc"] + "enum": [ + "kallisto", + "star", + "alevin", + "cellranger", + "cellrangerarc", + "universc" + ] }, "protocol": { "type": "string", @@ -120,12 +132,16 @@ "transcript_fasta": { "type": "string", "description": "A cDNA FASTA file", - "fa_icon": "fas fa-dna" + "fa_icon": "fas fa-dna", + "format": "file-path", + "exists": true }, "gtf": { "type": "string", "description": "Reference GTF annotation file", - "fa_icon": "fas fa-code-branch" + "fa_icon": "fas fa-code-branch", + "format": "file-path", + "exists": true }, "save_reference": { "type": "boolean", @@ -151,13 +167,17 @@ "salmon_index": { "type": "string", "description": "This can be used to specify a precomputed Salmon index in the pipeline, in order to skip the generation of required indices by Salmon itself.", - "fa_icon": "fas fa-fish" + "fa_icon": "fas fa-fish", + "format": "file-path", + "exists": true }, "txp2gene": { "type": "string", "description": "Path to transcript to gene mapping file. This allows the specification of a transcript to gene mapping file for Salmon Alevin and AlevinQC.", "help_text": "> This is not the same as the `kallisto_gene_map` parameter down below and is only used by the Salmon Alevin workflow.", - "fa_icon": "fas fa-map-marked-alt" + "fa_icon": "fas fa-map-marked-alt", + "format": "file-path", + "exists": true }, "simpleaf_rlen": { "type": "integer", @@ -177,7 +197,9 @@ "type": "string", "description": "Specify a path to the precomputed STAR index.", "help_text": "> NB: This has to be computed with STAR Version 2.7 or later, as STARsolo was only first supported by STAR Version 2.7.", - "fa_icon": "fas fa-asterisk" + "fa_icon": "fas fa-asterisk", + "format": "file-path", + "exists": true }, "star_ignore_sjdbgtf": { "type": "string", @@ -190,7 +212,11 @@ "star_feature": { "type": "string", "default": "Gene", - "enum": ["Gene", "GeneFull", "Gene Velocyto"], + "enum": [ + "Gene", + "GeneFull", + "Gene Velocyto" + ], "description": "Quantification type of different transcriptomic feature. Use `GeneFull` on pre-mRNA count for single-nucleus RNA-seq reads. Use `Gene Velocyto` to generate RNA velocity matrix.", "fa_icon": "fas fa-asterisk" } @@ -212,14 +238,22 @@ "kallisto_index": { "type": "string", "description": "Specify a path to the precomputed Kallisto index.", - "fa_icon": "fas fa-fish" + "fa_icon": "fas fa-fish", + "format": "file-path", + "exists": true }, "kb_workflow": { "type": "string", "default": "standard", "description": "Type of workflow. Use `lamanno` for RNA velocity based on La Manno et al. 2018 logic. Use `nucleus` for RNA velocity on single-nucleus RNA-seq reads. Use `kite` for feature barcoding. Use `kite: 10xFB` for 10x Genomics Feature Barcoding technology. (default: standard)", "fa_icon": "fas fa-fish", - "enum": ["standard", "lamanno", "nucleus", "kite", "kite: 10xFB"] + "enum": [ + "standard", + "lamanno", + "nucleus", + "kite", + "kite: 10xFB" + ] } } }, @@ -231,7 +265,9 @@ "properties": { "cellranger_index": { "type": "string", - "description": "Specify a pre-calculated cellranger index. Readily prepared indexes can be obtained from the 10x Genomics website. " + "description": "Specify a pre-calculated cellranger index. Readily prepared indexes can be obtained from the 10x Genomics website. ", + "format": "file-path", + "exists": true } } }, @@ -263,7 +299,9 @@ "properties": { "universc_index": { "type": "string", - "description": "Specify a pre-calculated cellranger index. Readily prepared indexes can be obtained from the 10x Genomics website." + "description": "Specify a pre-calculated cellranger index. Readily prepared indexes can be obtained from the 10x Genomics website.", + "format": "file-path", + "exists": true } } }, @@ -375,7 +413,14 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], + "enum": [ + "symlink", + "rellink", + "link", + "copy", + "copyNoFollow", + "move" + ], "hidden": true }, "email_on_fail": { @@ -417,6 +462,7 @@ "type": "string", "format": "file-path", "description": "Custom config file to supply to MultiQC.", + "exists": true, "fa_icon": "fas fa-cog", "hidden": true }, diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 77cd1d20..916533c2 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -1,12 +1,4 @@ -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - IMPORT MODULES / SUBWORKFLOWS / FUNCTIONS -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ - include { MULTIQC } from '../modules/nf-core/multiqc/main' - -include { INPUT_CHECK } from '../subworkflows/local/input_check' include { FASTQC_CHECK } from '../subworkflows/local/fastqc' include { KALLISTO_BUSTOOLS } from '../subworkflows/local/kallisto_bustools' include { SCRNASEQ_ALEVIN } from '../subworkflows/local/alevin' @@ -17,49 +9,33 @@ include { UNIVERSC_ALIGN } from "../subworkflows/local/align_universc" include { MTX_CONVERSION } from "../subworkflows/local/mtx_conversion" include { GTF_GENE_FILTER } from '../modules/local/gtf_gene_filter' -include { paramsSummaryMap } from 'plugin/nf-validation' include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_scrnaseq_pipeline' include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' - - workflow SCRNASEQ { take: - ch_samplesheet // channel: samplesheet read in from --input + ch_input // channel: samplesheet read in from --input main: + ch_fastq = Channel.empty() - // TODO: checks still necessary in favor of schema validation? - def checkPathParamList = [ - params.input, params.multiqc_config, params.fasta, params.gtf, - params.transcript_fasta, params.salmon_index, params.kallisto_index, - params.star_index, params.txp2gene, params.barcode_whitelist, params.cellranger_index, - params.universc_index - ] - for (param in checkPathParamList) { if (param) { file(param, checkIfExists: true) } } - - // Info required for completion email and summary - // TODO: Are this channels still necessary? protocol_config = WorkflowScrnaseq.getProtocol(workflow, log, params.aligner, params.protocol) if (protocol_config['protocol'] == 'auto' && params.aligner != "cellranger") { error "Only cellranger supports `protocol = 'auto'`. Please specify the protocol manually!" } // general input and params - ch_input = file(params.input) ch_genome_fasta = Channel.value(params.fasta ? file(params.fasta) : []) ch_gtf = params.gtf ? file(params.gtf) : [] ch_transcript_fasta = params.transcript_fasta ? file(params.transcript_fasta): [] ch_motifs = params.motifs ? file(params.motifs) : [] ch_cellrangerarc_config = params.cellrangerarc_config ? file(params.cellrangerarc_config) : [] ch_txp2gene = params.txp2gene ? file(params.txp2gene) : [] - ch_multiqc_alevin = Channel.empty() - ch_multiqc_star = Channel.empty() - ch_multiqc_cellranger = Channel.empty() + ch_multiqc_files = Channel.empty() if (params.barcode_whitelist) { ch_barcode_whitelist = file(params.barcode_whitelist) } else if (protocol_config.containsKey("whitelist")) { @@ -68,7 +44,6 @@ workflow SCRNASEQ { ch_barcode_whitelist = [] } - //kallisto params ch_kallisto_index = params.kallisto_index ? file(params.kallisto_index) : [] kb_workflow = params.kb_workflow @@ -89,22 +64,11 @@ workflow SCRNASEQ { ch_versions = Channel.empty() ch_mtx_matrices = Channel.empty() - // Check input files and stage input data - ch_fastq = INPUT_CHECK( ch_input ).reads - - ch_versions = ch_versions.mix(INPUT_CHECK.out.versions) - // TODO: OPTIONAL, you can use nf-validation plugin to create an input channel from the samplesheet with Channel.fromSamplesheet("input") - // See the documentation https://nextflow-io.github.io/nf-validation/samplesheets/fromSamplesheet/ - // ! There is currently no tooling to help you write a sample sheet schema - // Run FastQC - ch_multiqc_fastqc = Channel.empty() if (!params.skip_fastqc) { FASTQC_CHECK ( ch_fastq ) ch_versions = ch_versions.mix(FASTQC_CHECK.out.fastqc_version) - ch_multiqc_fastqc = FASTQC_CHECK.out.fastqc_zip - } else { - ch_multiqc_fastqc = Channel.empty() + ch_multiqc_files = ch_multiqc_files.mix(FASTQC_CHECK.out.fastqc_zip) } ch_filter_gtf = GTF_GENE_FILTER ( ch_genome_fasta, ch_gtf ).gtf @@ -138,7 +102,7 @@ workflow SCRNASEQ { ch_fastq ) ch_versions = ch_versions.mix(SCRNASEQ_ALEVIN.out.ch_versions) - ch_multiqc_alevin = SCRNASEQ_ALEVIN.out.alevin_results + ch_multiqc_files = ch_multiqc_files.mix(SCRNASEQ_ALEVIN.out.alevin_results) ch_mtx_matrices = ch_mtx_matrices.mix(SCRNASEQ_ALEVIN.out.alevin_results) } @@ -157,7 +121,7 @@ workflow SCRNASEQ { ch_versions = ch_versions.mix(STARSOLO.out.ch_versions) ch_mtx_matrices = ch_mtx_matrices.mix(STARSOLO.out.star_counts) ch_star_index = STARSOLO.out.star_index - ch_multiqc_star = STARSOLO.out.for_multiqc + ch_multiqc_files = ch_multiqc_files.mix(STARSOLO.out.for_multiqc) } // Run cellranger pipeline @@ -172,9 +136,9 @@ workflow SCRNASEQ { ch_versions = ch_versions.mix(CELLRANGER_ALIGN.out.ch_versions) ch_mtx_matrices = ch_mtx_matrices.mix(CELLRANGER_ALIGN.out.cellranger_out) ch_star_index = CELLRANGER_ALIGN.out.star_index - ch_multiqc_cellranger = CELLRANGER_ALIGN.out.cellranger_out.map{ + ch_multiqc_files = ch_multiqc_files.mix(CELLRANGER_ALIGN.out.cellranger_out.map{ meta, outs -> outs.findAll{ it -> it.name == "web_summary.html"} - } + }) } // Run universc pipeline @@ -204,16 +168,17 @@ workflow SCRNASEQ { ch_mtx_matrices = ch_mtx_matrices.mix(CELLRANGERARC_ALIGN.out.cellranger_arc_out) } - // Run mtx to h5ad conversion subworkflow - MTX_CONVERSION ( - ch_mtx_matrices, - ch_input, - ch_txp2gene, - ch_star_index - ) + // TODO + // // Run mtx to h5ad conversion subworkflow + // MTX_CONVERSION ( + // ch_mtx_matrices, + // ch_input, + // ch_txp2gene, + // ch_star_index + // ) - //Add Versions from MTX Conversion workflow too - ch_versions.mix(MTX_CONVERSION.out.ch_versions) + // //Add Versions from MTX Conversion workflow too + // ch_versions.mix(MTX_CONVERSION.out.ch_versions) // // MODULE: MultiQC @@ -240,9 +205,3 @@ workflow SCRNASEQ { multiqc_report = MULTIQC.out.report.toList() // channel: /path/to/multiqc_report.html versions = ch_versions // channel: [ path(versions.yml) ] } - -/* -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ - THE END -~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ -*/ From 94600114cc8cfdb60f6d9063f1f1b1ea8634f869 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 10:14:59 +0100 Subject: [PATCH 071/111] Enable workflow --- main.nf | 40 +++++++++++++++++++--------------------- workflows/scrnaseq.nf | 3 +-- 2 files changed, 20 insertions(+), 23 deletions(-) diff --git a/main.nf b/main.nf index 4f96908f..3471e5b0 100644 --- a/main.nf +++ b/main.nf @@ -82,27 +82,25 @@ workflow { params.input ) - PIPELINE_INITIALISATION.out.samplesheet.view() - - // // - // // WORKFLOW: Run main workflow - // // - // NFCORE_SCRNASEQ ( - // PIPELINE_INITIALISATION.out.samplesheet.view() - // ) - - // // - // // SUBWORKFLOW: Run completion tasks - // // - // PIPELINE_COMPLETION ( - // params.email, - // params.email_on_fail, - // params.plaintext_email, - // params.outdir, - // params.monochrome_logs, - // params.hook_url, - // NFCORE_SCRNASEQ.out.multiqc_report - // ) + // + // WORKFLOW: Run main workflow + // + NFCORE_SCRNASEQ ( + PIPELINE_INITIALISATION.out.samplesheet + ) + + // + // SUBWORKFLOW: Run completion tasks + // + PIPELINE_COMPLETION ( + params.email, + params.email_on_fail, + params.plaintext_email, + params.outdir, + params.monochrome_logs, + params.hook_url, + NFCORE_SCRNASEQ.out.multiqc_report + ) } /* diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 916533c2..ab1fa3a1 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -18,10 +18,9 @@ include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' workflow SCRNASEQ { take: - ch_input // channel: samplesheet read in from --input + ch_fastq main: - ch_fastq = Channel.empty() protocol_config = WorkflowScrnaseq.getProtocol(workflow, log, params.aligner, params.protocol) if (protocol_config['protocol'] == 'auto' && params.aligner != "cellranger") { From c78f4bbb1cc24afc18e2ee8afd59f553f00a083f Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 10:18:02 +0100 Subject: [PATCH 072/111] pre-commit --- assets/schema_input.json | 27 +++------- modules.json | 76 +++++++------------------- modules/nf-core/universc/CITATION.cff | 78 +++++++++++++-------------- nextflow_schema.json | 37 ++----------- workflows/scrnaseq.nf | 26 +++++---- 5 files changed, 84 insertions(+), 160 deletions(-) diff --git a/assets/schema_input.json b/assets/schema_input.json index af6ccfa3..a531769f 100644 --- a/assets/schema_input.json +++ b/assets/schema_input.json @@ -11,9 +11,7 @@ "type": "string", "pattern": "^\\S+$", "errorMessage": "Sample name must be provided and cannot contain spaces", - "meta": [ - "id" - ] + "meta": ["id"] }, "fastq_1": { "type": "string", @@ -32,31 +30,18 @@ "expected_cells": { "type": "integer", "errorMessage": "Expected cells must be an Integer", - "meta": [ - "expected_cells" - ] + "meta": ["expected_cells"] }, "seq_center": { "type": "string", - "meta": [ - "seq_center" - ] + "meta": ["seq_center"] }, "sample_type": { "type": "string", - "enum": [ - "atac", - "gex" - ], - "meta": [ - "sample_type" - ] + "enum": ["atac", "gex"], + "meta": ["sample_type"] } }, - "required": [ - "sample", - "fastq_1", - "fastq_2" - ] + "required": ["sample", "fastq_1", "fastq_2"] } } diff --git a/modules.json b/modules.json index d81f322c..e1234f4c 100644 --- a/modules.json +++ b/modules.json @@ -8,114 +8,82 @@ "cellranger/count": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellranger/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellranger/mkref": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/count": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/mkref": { "branch": "master", "git_sha": "4196b1b2e7ce265892f3979eabf7a9ddc030702f", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "custom/dumpsoftwareversions": { "branch": "master", "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gffread": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "kallistobustools/count": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "kallistobustools/ref": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "9e71d8519dfbfc328c078bba14d4bd4c99e39a94", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "star/genomegenerate": { "branch": "master", "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "universc": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "unzip": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -124,23 +92,17 @@ "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] } } } diff --git a/modules/nf-core/universc/CITATION.cff b/modules/nf-core/universc/CITATION.cff index b00957d1..35e281e6 100644 --- a/modules/nf-core/universc/CITATION.cff +++ b/modules/nf-core/universc/CITATION.cff @@ -1,23 +1,6 @@ cff-version: 1.2.0 message: "If you use this software, please cite it as below." authors: - - given-names: "S. Thomas" - family-names: "Kelly" - email: "tom.kelly@riken.jp" - affiliation: "Center for Integrative Medical Sciences, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" - orcid: "https://orcid.org/0000-0003-3904-6690" - - family-names: "Battenberg" - given-names: "Kai" - email: "kai.battenberg@riken.jp" - affiliation: "Center for Sustainable Resource Science, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" - orcid: "http://orcid.org/0000-0001-7517-2657" -version: 1.2.5.1 -doi: 10.1101/2021.01.19.427209 -date-released: 2021-02-14 -url: "https://github.com/minoda-lab/universc" -preferred-citation: - type: article - authors: - given-names: "S. Thomas" family-names: "Kelly" email: "tom.kelly@riken.jp" @@ -27,25 +10,42 @@ preferred-citation: given-names: "Kai" email: "kai.battenberg@riken.jp" affiliation: "Center for Sustainable Resource Science, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" - orcid: "https://orcid.org/http://orcid.org/0000-0001-7517-2657" - - family-names: "Hetherington" - given-names: "Nicola A." - affiliation: "Center for Integrative Medical Sciences, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" - orcid: "http://orcid.org/0000-0001-8802-2906" - - family-names: "Hayashi" - given-names: "Makoto" - affiliation: "Center for Sustainable Resource Science, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" - orcid: "http://orcid.org/0000-0001-6389-4265" - - given-names: "Aki" - family-names: "Minoda" - email: "akiko.minoda@riken.jp" - affiliation: Center for Integrative Medical Sciences, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" - orcid: "http://orcid.org/0000-0002-2927-5791" - doi: "10.1101/2021.01.19.427209" - title: "UniverSC: a flexible cross-platform single-cell data processing pipeline" - year: "2021" - journal: "bioRxiv" - start: 2021.01.19.427209 - volume: - issue: - month: 1 + orcid: "http://orcid.org/0000-0001-7517-2657" +version: 1.2.5.1 +doi: 10.1101/2021.01.19.427209 +date-released: 2021-02-14 +url: "https://github.com/minoda-lab/universc" +preferred-citation: + type: article + authors: + - given-names: "S. Thomas" + family-names: "Kelly" + email: "tom.kelly@riken.jp" + affiliation: "Center for Integrative Medical Sciences, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" + orcid: "https://orcid.org/0000-0003-3904-6690" + - family-names: "Battenberg" + given-names: "Kai" + email: "kai.battenberg@riken.jp" + affiliation: "Center for Sustainable Resource Science, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" + orcid: "https://orcid.org/http://orcid.org/0000-0001-7517-2657" + - family-names: "Hetherington" + given-names: "Nicola A." + affiliation: "Center for Integrative Medical Sciences, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" + orcid: "http://orcid.org/0000-0001-8802-2906" + - family-names: "Hayashi" + given-names: "Makoto" + affiliation: "Center for Sustainable Resource Science, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" + orcid: "http://orcid.org/0000-0001-6389-4265" + - given-names: "Aki" + family-names: "Minoda" + email: "akiko.minoda@riken.jp" + affiliation: Center for Integrative Medical Sciences, RIKEN, Suehiro-cho-1-7-22, Tsurumi Ward, Yokohama, Japan" + orcid: "http://orcid.org/0000-0002-2927-5791" + doi: "10.1101/2021.01.19.427209" + title: "UniverSC: a flexible cross-platform single-cell data processing pipeline" + year: "2021" + journal: "bioRxiv" + start: 2021.01.19.427209 + volume: + issue: + month: 1 diff --git a/nextflow_schema.json b/nextflow_schema.json index e9fef834..649b9b18 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -10,10 +10,7 @@ "type": "object", "fa_icon": "fas fa-terminal", "description": "Define where the pipeline should find input data and save output data.", - "required": [ - "input", - "outdir" - ], + "required": ["input", "outdir"], "properties": { "input": { "type": "string", @@ -64,14 +61,7 @@ "default": "alevin", "help_text": "The workflow can handle three types of methods:\n\n- Kallisto/Bustools\n- Salmon Alevin + AlevinQC\n- STARsolo\n\nTo choose which one to use, please specify either `alevin`, `star` or `kallisto` as a parameter option for `--aligner`. By default, the pipeline runs the `alevin` option. Note that specifying another aligner option also requires choosing appropriate parameters (see below) for the selected option.", "fa_icon": "fas fa-align-center", - "enum": [ - "kallisto", - "star", - "alevin", - "cellranger", - "cellrangerarc", - "universc" - ] + "enum": ["kallisto", "star", "alevin", "cellranger", "cellrangerarc", "universc"] }, "protocol": { "type": "string", @@ -212,11 +202,7 @@ "star_feature": { "type": "string", "default": "Gene", - "enum": [ - "Gene", - "GeneFull", - "Gene Velocyto" - ], + "enum": ["Gene", "GeneFull", "Gene Velocyto"], "description": "Quantification type of different transcriptomic feature. Use `GeneFull` on pre-mRNA count for single-nucleus RNA-seq reads. Use `Gene Velocyto` to generate RNA velocity matrix.", "fa_icon": "fas fa-asterisk" } @@ -247,13 +233,7 @@ "default": "standard", "description": "Type of workflow. Use `lamanno` for RNA velocity based on La Manno et al. 2018 logic. Use `nucleus` for RNA velocity on single-nucleus RNA-seq reads. Use `kite` for feature barcoding. Use `kite: 10xFB` for 10x Genomics Feature Barcoding technology. (default: standard)", "fa_icon": "fas fa-fish", - "enum": [ - "standard", - "lamanno", - "nucleus", - "kite", - "kite: 10xFB" - ] + "enum": ["standard", "lamanno", "nucleus", "kite", "kite: 10xFB"] } } }, @@ -413,14 +393,7 @@ "description": "Method used to save pipeline results to output directory.", "help_text": "The Nextflow `publishDir` option specifies which intermediate files should be saved to the output directory. This option tells the pipeline what method should be used to move these files. See [Nextflow docs](https://www.nextflow.io/docs/latest/process.html#publishdir) for details.", "fa_icon": "fas fa-copy", - "enum": [ - "symlink", - "rellink", - "link", - "copy", - "copyNoFollow", - "move" - ], + "enum": ["symlink", "rellink", "link", "copy", "copyNoFollow", "move"], "hidden": true }, "email_on_fail": { diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index ab1fa3a1..48b205af 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -43,6 +43,10 @@ workflow SCRNASEQ { ch_barcode_whitelist = [] } + // samplesheet - this is passed to the MTX conversion functions to add metadata to the + // AnnData objects. + ch_input = file(params.input) + //kallisto params ch_kallisto_index = params.kallisto_index ? file(params.kallisto_index) : [] kb_workflow = params.kb_workflow @@ -167,17 +171,17 @@ workflow SCRNASEQ { ch_mtx_matrices = ch_mtx_matrices.mix(CELLRANGERARC_ALIGN.out.cellranger_arc_out) } - // TODO - // // Run mtx to h5ad conversion subworkflow - // MTX_CONVERSION ( - // ch_mtx_matrices, - // ch_input, - // ch_txp2gene, - // ch_star_index - // ) - - // //Add Versions from MTX Conversion workflow too - // ch_versions.mix(MTX_CONVERSION.out.ch_versions) + TODO + // Run mtx to h5ad conversion subworkflow + MTX_CONVERSION ( + ch_mtx_matrices, + ch_input, + ch_txp2gene, + ch_star_index + ) + + //Add Versions from MTX Conversion workflow too + ch_versions.mix(MTX_CONVERSION.out.ch_versions) // // MODULE: MultiQC From 66bbb99ca71929effcb7062d66e92d6b55f84d30 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 10:24:09 +0100 Subject: [PATCH 073/111] Fix CI --- .github/workflows/ci.yml | 4 ---- 1 file changed, 4 deletions(-) diff --git a/.github/workflows/ci.yml b/.github/workflows/ci.yml index 746f26a2..b5524e7d 100644 --- a/.github/workflows/ci.yml +++ b/.github/workflows/ci.yml @@ -47,10 +47,6 @@ jobs: with: version: "${{ matrix.NXF_VER }}" - - name: Run pipeline with test data - # TODO nf-core: You can customise CI pipeline run tests as required - # For example: adding multiple test runs with different parameters - # Remember that you can parallelise this by using strategy.matrix - name: Cache nf-test installation id: cache-software uses: actions/cache@v3 From 4203d1424d7c87a3ee8d1e179f474eae0aff9e87 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 10:28:04 +0100 Subject: [PATCH 074/111] Update modules and fix linting --- lib/{WorkflowScrnaseq.groovy => Utils.groovy} | 0 modules.json | 7 +- modules/nf-core/cellranger/count/main.nf | 2 +- modules/nf-core/cellranger/count/meta.yml | 3 +- .../count/templates/cellranger_count.py | 2 +- .../cellranger/count/tests/main.nf.test | 101 ++++++++++++++++++ .../cellranger/count/tests/main.nf.test.snap | 63 +++++++++++ .../cellranger/count/tests/nextflow.config | 24 +++++ .../nf-core/cellranger/count/tests/tags.yml | 2 + workflows/scrnaseq.nf | 2 +- 10 files changed, 196 insertions(+), 10 deletions(-) rename lib/{WorkflowScrnaseq.groovy => Utils.groovy} (100%) create mode 100644 modules/nf-core/cellranger/count/tests/main.nf.test create mode 100644 modules/nf-core/cellranger/count/tests/main.nf.test.snap create mode 100644 modules/nf-core/cellranger/count/tests/nextflow.config create mode 100644 modules/nf-core/cellranger/count/tests/tags.yml diff --git a/lib/WorkflowScrnaseq.groovy b/lib/Utils.groovy similarity index 100% rename from lib/WorkflowScrnaseq.groovy rename to lib/Utils.groovy diff --git a/modules.json b/modules.json index e1234f4c..805606f5 100644 --- a/modules.json +++ b/modules.json @@ -7,7 +7,7 @@ "nf-core": { "cellranger/count": { "branch": "master", - "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", + "git_sha": "a2dfd9a0b2e192243695711c723d652959de39fc", "installed_by": ["modules"] }, "cellranger/mkgtf": { @@ -35,11 +35,6 @@ "git_sha": "4196b1b2e7ce265892f3979eabf7a9ddc030702f", "installed_by": ["modules"] }, - "custom/dumpsoftwareversions": { - "branch": "master", - "git_sha": "8ec825f465b9c17f9d83000022995b4f7de6fe93", - "installed_by": ["modules"] - }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", diff --git a/modules/nf-core/cellranger/count/main.nf b/modules/nf-core/cellranger/count/main.nf index d7a191fc..42aa09c9 100644 --- a/modules/nf-core/cellranger/count/main.nf +++ b/modules/nf-core/cellranger/count/main.nf @@ -32,7 +32,7 @@ process CELLRANGER_COUNT { def prefix = task.ext.prefix ?: "${meta.id}" """ mkdir -p "${prefix}/outs/" - touch ${prefix}/outs/fake_file.txt + echo "$prefix" > ${prefix}/outs/fake_file.txt cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/cellranger/count/meta.yml b/modules/nf-core/cellranger/count/meta.yml index c1f4259a..1f1768a8 100644 --- a/modules/nf-core/cellranger/count/meta.yml +++ b/modules/nf-core/cellranger/count/meta.yml @@ -10,7 +10,8 @@ tools: homepage: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger documentation: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_ov tool_dev_url: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_ov - licence: 10x Genomics EULA + licence: + - 10x Genomics EULA input: - meta: type: map diff --git a/modules/nf-core/cellranger/count/templates/cellranger_count.py b/modules/nf-core/cellranger/count/templates/cellranger_count.py index 1527ba76..53360f23 100644 --- a/modules/nf-core/cellranger/count/templates/cellranger_count.py +++ b/modules/nf-core/cellranger/count/templates/cellranger_count.py @@ -36,7 +36,7 @@ def chunk_iter(seq, size): # do not match "SRR12345", "file_INFIXR12", etc filename_pattern = r'([^a-zA-Z0-9])R1([^a-zA-Z0-9])' -for i, (r1, r2) in enumerate(chunk_iter(fastqs, 2)): +for i, (r1, r2) in enumerate(chunk_iter(fastqs, 2), start=1): # double escapes are required because nextflow processes this python 'template' if re.sub(filename_pattern, r'\\1R2\\2', r1.name) != r2.name: raise AssertionError( diff --git a/modules/nf-core/cellranger/count/tests/main.nf.test b/modules/nf-core/cellranger/count/tests/main.nf.test new file mode 100644 index 00000000..c779b89e --- /dev/null +++ b/modules/nf-core/cellranger/count/tests/main.nf.test @@ -0,0 +1,101 @@ +nextflow_process { + + name "Test Process CELLRANGER_COUNT" + script "../main.nf" + process "CELLRANGER_COUNT" + + tag "modules" + tag "modules_nfcore" + tag "cellranger" + tag "cellranger/count" + tag "cellranger/mkref" + tag "cellranger/mkgtf" + + setup { + config "./nextflow.config" + + run("CELLRANGER_MKGTF") { + script "../../mkgtf/main.nf" + process { + """ + input[0] = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true) + """ + } + } + run("CELLRANGER_MKREF") { + script "../../mkref/main.nf" + process { + """ + input[0] = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + input[1] = CELLRANGER_MKGTF.out.gtf + input[2] = "homo_sapiens_chr22_reference" + """ + } + } + } + + test("10x example file") { + config "./nextflow.config" + when { + process { + """ + input[0] = [ + [ id:'test_10x', single_end:false, strandedness:'auto' ], // meta map + [ + file(params.test_data['homo_sapiens']['10xgenomics']['cellranger']['test_10x_5k_cmvpos_tcells_gex1_fastq_1_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['10xgenomics']['cellranger']['test_10x_5k_cmvpos_tcells_gex1_fastq_2_gz'], checkIfExists: true) + ] + ] + input[1] = CELLRANGER_MKREF.out.reference + """ + } + } + + then { + assertAll( + { assert process.success }, + { + assert snapshot( + process.out.versions, + process.out.outs[0][1].findAll { file(it).name !in [ + 'web_summary.html', // unstable checksum + 'barcodes.tsv.gz' // empty file in test data -> would raise linting error + ]} + ).match() + }, + { assert file(process.out.outs.get(0).get(1).find { file(it).name == 'web_summary.html' }).exists() }, + { assert file(process.out.outs.get(0).get(1).find { file(it).name == 'barcodes.tsv.gz' }).exists() } + ) + } + + } + + test("10x example file - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [ + [ id:'test_10x', single_end:false, strandedness:'auto' ], // meta map + [ + file(params.test_data['homo_sapiens']['10xgenomics']['cellranger']['test_10x_5k_cmvpos_tcells_gex1_fastq_1_gz'], checkIfExists: true), + file(params.test_data['homo_sapiens']['10xgenomics']['cellranger']['test_10x_5k_cmvpos_tcells_gex1_fastq_2_gz'], checkIfExists: true) + ] + ] + input[1] = CELLRANGER_MKREF.out.reference + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/cellranger/count/tests/main.nf.test.snap b/modules/nf-core/cellranger/count/tests/main.nf.test.snap new file mode 100644 index 00000000..7eafafd0 --- /dev/null +++ b/modules/nf-core/cellranger/count/tests/main.nf.test.snap @@ -0,0 +1,63 @@ +{ + "10x example file": { + "content": [ + [ + "versions.yml:md5,ec6a0c18d2a8332e42956d07215a657c" + ], + [ + "filtered_feature_bc_matrix.h5:md5,cd440a402b661dc307356057d0dd3348", + "features.tsv.gz:md5,9f93621be0bede2b75596ad255607633", + "matrix.mtx.gz:md5,1736b88199c1dd71e907f9b93becc4f8", + "metrics_summary.csv:md5,c295fe3ccbb6ce118482964ca9c748d4", + "molecule_info.h5:md5,6204ac0b164b6e06e65954d072afe599", + "possorted_genome_bam.bam:md5,32fe604968326d98e8c0c36ce3c5ee8d", + "possorted_genome_bam.bam.bai:md5,e37e792ca1b528e64cd57f7d70dae3ed", + "raw_feature_bc_matrix.h5:md5,8521dbdf077e029ae35e682d746eb79d", + "features.tsv.gz:md5,9f93621be0bede2b75596ad255607633", + "matrix.mtx.gz:md5,6294c0359b29a86ba7b06e07d4b8a134" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-06T09:30:22.104987533" + }, + "10x example file - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test_10x", + "single_end": false, + "strandedness": "auto" + }, + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" + ] + ], + "1": [ + "versions.yml:md5,30cee1a9146b01c48d9b1db6bbe813b6" + ], + "outs": [ + [ + { + "id": "test_10x", + "single_end": false, + "strandedness": "auto" + }, + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" + ] + ], + "versions": [ + "versions.yml:md5,30cee1a9146b01c48d9b1db6bbe813b6" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-05T17:16:12.322822411" + } +} \ No newline at end of file diff --git a/modules/nf-core/cellranger/count/tests/nextflow.config b/modules/nf-core/cellranger/count/tests/nextflow.config new file mode 100644 index 00000000..2859b34a --- /dev/null +++ b/modules/nf-core/cellranger/count/tests/nextflow.config @@ -0,0 +1,24 @@ +process { + withName: CELLRANGER_MKGTF { + ext.args = '--attribute=gene_biotype:protein_coding \ + --attribute=gene_biotype:lincRNA \ + --attribute=gene_biotype:antisense \ + --attribute=gene_biotype:IG_LV_gene \ + --attribute=gene_biotype:IG_V_gene \ + --attribute=gene_biotype:IG_V_pseudogene \ + --attribute=gene_biotype:IG_D_gene \ + --attribute=gene_biotype:IG_J_gene \ + --attribute=gene_biotype:IG_J_pseudogene \ + --attribute=gene_biotype:IG_C_gene \ + --attribute=gene_biotype:IG_C_pseudogene \ + --attribute=gene_biotype:TR_V_gene \ + --attribute=gene_biotype:TR_V_pseudogene \ + --attribute=gene_biotype:TR_D_gene \ + --attribute=gene_biotype:TR_J_gene \ + --attribute=gene_biotype:TR_J_pseudogene \ + --attribute=gene_biotype:TR_C_gene' + } + withName: CELLRANGER_COUNT { + ext.args = '--chemistry SC3Pv3' + } +} diff --git a/modules/nf-core/cellranger/count/tests/tags.yml b/modules/nf-core/cellranger/count/tests/tags.yml new file mode 100644 index 00000000..a29a7044 --- /dev/null +++ b/modules/nf-core/cellranger/count/tests/tags.yml @@ -0,0 +1,2 @@ +cellranger/count: + - "modules/nf-core/cellranger/count/**" diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index 48b205af..e1db493a 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -22,7 +22,7 @@ workflow SCRNASEQ { main: - protocol_config = WorkflowScrnaseq.getProtocol(workflow, log, params.aligner, params.protocol) + protocol_config = Utils.getProtocol(workflow, log, params.aligner, params.protocol) if (protocol_config['protocol'] == 'auto' && params.aligner != "cellranger") { error "Only cellranger supports `protocol = 'auto'`. Please specify the protocol manually!" } From fcc405b6aec05948de2797b3bc1a52dd23e68f48 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 10:46:35 +0100 Subject: [PATCH 075/111] Fix multiqc --- workflows/scrnaseq.nf | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index e1db493a..d077c1f0 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -71,7 +71,7 @@ workflow SCRNASEQ { if (!params.skip_fastqc) { FASTQC_CHECK ( ch_fastq ) ch_versions = ch_versions.mix(FASTQC_CHECK.out.fastqc_version) - ch_multiqc_files = ch_multiqc_files.mix(FASTQC_CHECK.out.fastqc_zip) + ch_multiqc_files = ch_multiqc_files.mix(FASTQC_CHECK.out.fastqc_zip.map{ meta, it -> it }) } ch_filter_gtf = GTF_GENE_FILTER ( ch_genome_fasta, ch_gtf ).gtf @@ -171,7 +171,6 @@ workflow SCRNASEQ { ch_mtx_matrices = ch_mtx_matrices.mix(CELLRANGERARC_ALIGN.out.cellranger_arc_out) } - TODO // Run mtx to h5ad conversion subworkflow MTX_CONVERSION ( ch_mtx_matrices, From b6b80a9ae035402a325ff6cdcde265f8fb8abb6f Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 11:10:27 +0100 Subject: [PATCH 076/111] Fix nf-test --- .gitignore | 2 +- nextflow.config | 4 ++++ subworkflows/local/starsolo.nf | 4 ++-- tests/main_pipeline_alevin.test.snap | 10 +++++----- tests/main_pipeline_cellranger.test.snap | 10 +++++----- tests/main_pipeline_kallisto.test.snap | 10 +++++----- tests/main_pipeline_star.test.snap | 10 +++++----- workflows/scrnaseq.nf | 2 +- 8 files changed, 28 insertions(+), 24 deletions(-) diff --git a/.gitignore b/.gitignore index a9ba2c52..61b9470a 100644 --- a/.gitignore +++ b/.gitignore @@ -9,4 +9,4 @@ testing* log/ reports/ testme.sh -.nf-test/ +.nf-test* diff --git a/nextflow.config b/nextflow.config index 495a8414..bf24e8a9 100644 --- a/nextflow.config +++ b/nextflow.config @@ -95,6 +95,10 @@ params { validationShowHiddenParams = false validate_params = true + // TODO temporary workaround a warning + // not used anywhere and should not be necessary anymore after a nf-validation plugin update + monochromeLogs = null + } // Load base.config by default for all pipelines diff --git a/subworkflows/local/starsolo.nf b/subworkflows/local/starsolo.nf index 47b2e757..e6c95409 100644 --- a/subworkflows/local/starsolo.nf +++ b/subworkflows/local/starsolo.nf @@ -57,8 +57,8 @@ workflow STARSOLO { emit: ch_versions // get rid of meta for star index - star_index = star_index.map{ meta, index -> index} + star_index = star_index.map{ meta, index -> index } star_result = STAR_ALIGN.out.tab star_counts = STAR_ALIGN.out.counts - for_multiqc = STAR_ALIGN.out.log_final + for_multiqc = STAR_ALIGN.out.log_final.map{ meta, it -> it } } diff --git a/tests/main_pipeline_alevin.test.snap b/tests/main_pipeline_alevin.test.snap index 9d194012..1ea49820 100644 --- a/tests/main_pipeline_alevin.test.snap +++ b/tests/main_pipeline_alevin.test.snap @@ -3,19 +3,19 @@ "content": [ { "stderr": [ - + ], "errorReport": "", "exitStatus": 0, "failed": false, "stdout": [ - + ], "errorMessage": "", "trace": { "tasksFailed": 0, - "tasksCount": 16, - "tasksSucceeded": 16 + "tasksCount": 14, + "tasksSucceeded": 14 }, "name": "workflow", "success": true @@ -29,4 +29,4 @@ ], "timestamp": "2024-01-19T10:28:35.652763852" } -} \ No newline at end of file +} diff --git a/tests/main_pipeline_cellranger.test.snap b/tests/main_pipeline_cellranger.test.snap index 5f88b954..865d651b 100644 --- a/tests/main_pipeline_cellranger.test.snap +++ b/tests/main_pipeline_cellranger.test.snap @@ -3,19 +3,19 @@ "content": [ { "stderr": [ - + ], "errorReport": "", "exitStatus": 0, "failed": false, "stdout": [ - + ], "errorMessage": "", "trace": { "tasksFailed": 0, - "tasksCount": 15, - "tasksSucceeded": 15 + "tasksCount": 13, + "tasksSucceeded": 13 }, "name": "workflow", "success": true @@ -37,4 +37,4 @@ ], "timestamp": "2024-01-22T15:19:20.134275449" } -} \ No newline at end of file +} diff --git a/tests/main_pipeline_kallisto.test.snap b/tests/main_pipeline_kallisto.test.snap index b0fb50bb..e57b77af 100644 --- a/tests/main_pipeline_kallisto.test.snap +++ b/tests/main_pipeline_kallisto.test.snap @@ -3,19 +3,19 @@ "content": [ { "stderr": [ - + ], "errorReport": "", "exitStatus": 0, "failed": false, "stdout": [ - + ], "errorMessage": "", "trace": { "tasksFailed": 0, - "tasksCount": 14, - "tasksSucceeded": 14 + "tasksCount": 12, + "tasksSucceeded": 12 }, "name": "workflow", "success": true @@ -31,4 +31,4 @@ ], "timestamp": "2024-01-23T12:19:47.921508953" } -} \ No newline at end of file +} diff --git a/tests/main_pipeline_star.test.snap b/tests/main_pipeline_star.test.snap index ef086f60..eaa97b8b 100644 --- a/tests/main_pipeline_star.test.snap +++ b/tests/main_pipeline_star.test.snap @@ -3,19 +3,19 @@ "content": [ { "stderr": [ - + ], "errorReport": "", "exitStatus": 0, "failed": false, "stdout": [ - + ], "errorMessage": "", "trace": { "tasksFailed": 0, - "tasksCount": 14, - "tasksSucceeded": 14 + "tasksCount": 12, + "tasksSucceeded": 12 }, "name": "workflow", "success": true @@ -35,4 +35,4 @@ ], "timestamp": "2024-01-19T15:46:22.470527538" } -} \ No newline at end of file +} diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index d077c1f0..f2a0ba3f 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -105,7 +105,7 @@ workflow SCRNASEQ { ch_fastq ) ch_versions = ch_versions.mix(SCRNASEQ_ALEVIN.out.ch_versions) - ch_multiqc_files = ch_multiqc_files.mix(SCRNASEQ_ALEVIN.out.alevin_results) + ch_multiqc_files = ch_multiqc_files.mix(SCRNASEQ_ALEVIN.out.alevin_results.map{ meta, it -> it }) ch_mtx_matrices = ch_mtx_matrices.mix(SCRNASEQ_ALEVIN.out.alevin_results) } From 273a157027ed924329dbc4fa6923ff761009c6fa Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 11:19:47 +0100 Subject: [PATCH 077/111] Fix nf-test --- tests/main_pipeline_alevin.test | 2 +- tests/main_pipeline_cellranger.test | 2 +- tests/main_pipeline_kallisto.test | 2 +- tests/main_pipeline_star.test | 2 +- 4 files changed, 4 insertions(+), 4 deletions(-) diff --git a/tests/main_pipeline_alevin.test b/tests/main_pipeline_alevin.test index 303a625b..4bbd3cfb 100644 --- a/tests/main_pipeline_alevin.test +++ b/tests/main_pipeline_alevin.test @@ -30,7 +30,7 @@ nextflow_pipeline { {assert workflow.success}, // How many tasks were executed? - {assert workflow.trace.tasks().size() == 16}, + {assert workflow.trace.tasks().size() == 14}, // How many results were produced? {assert path("${outputDir}/results_alevin").list().size() == 5}, diff --git a/tests/main_pipeline_cellranger.test b/tests/main_pipeline_cellranger.test index 996c0f76..b7b34fa5 100644 --- a/tests/main_pipeline_cellranger.test +++ b/tests/main_pipeline_cellranger.test @@ -30,7 +30,7 @@ nextflow_pipeline { {assert workflow.success}, // How many tasks were executed? - {assert workflow.trace.tasks().size() == 15}, + {assert workflow.trace.tasks().size() == 13}, // How many results were produced? {assert path("${outputDir}/results_cellranger").list().size() == 4}, diff --git a/tests/main_pipeline_kallisto.test b/tests/main_pipeline_kallisto.test index 98ede8c4..ae6675b1 100644 --- a/tests/main_pipeline_kallisto.test +++ b/tests/main_pipeline_kallisto.test @@ -30,7 +30,7 @@ nextflow_pipeline { {assert workflow.success}, // How many tasks were executed? - {assert workflow.trace.tasks().size() == 14}, + {assert workflow.trace.tasks().size() == 12}, // How many results were produced? {assert path("${outputDir}/results_kallisto").list().size() == 4}, diff --git a/tests/main_pipeline_star.test b/tests/main_pipeline_star.test index 6616ea87..111c5243 100644 --- a/tests/main_pipeline_star.test +++ b/tests/main_pipeline_star.test @@ -30,7 +30,7 @@ nextflow_pipeline { {assert workflow.success}, // How many tasks were executed? - {assert workflow.trace.tasks().size() == 14}, + {assert workflow.trace.tasks().size() == 12}, // How many results were produced? {assert path("${outputDir}/results_star").list().size() == 4}, From 0c53b9b1c11cec848e3870730e549cc798da1fa6 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 11:34:27 +0100 Subject: [PATCH 078/111] Fix lint --- .nf-core.yml | 2 ++ modules.json | 2 +- modules/nf-core/multiqc/tests/main.nf.test.snap | 12 ++++++------ 3 files changed, 9 insertions(+), 7 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 738ad918..6a1c8178 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -3,3 +3,5 @@ lint: template_strings: False files_unchanged: - .github/ISSUE_TEMPLATE/bug_report.yml + files_exist: + - lib/Utils.groovy diff --git a/modules.json b/modules.json index 805606f5..8aa2641e 100644 --- a/modules.json +++ b/modules.json @@ -62,7 +62,7 @@ }, "multiqc": { "branch": "master", - "git_sha": "9e71d8519dfbfc328c078bba14d4bd4c99e39a94", + "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", "installed_by": ["modules"] }, "star/genomegenerate": { diff --git a/modules/nf-core/multiqc/tests/main.nf.test.snap b/modules/nf-core/multiqc/tests/main.nf.test.snap index 549ba79c..bfebd802 100644 --- a/modules/nf-core/multiqc/tests/main.nf.test.snap +++ b/modules/nf-core/multiqc/tests/main.nf.test.snap @@ -2,14 +2,14 @@ "multiqc_versions_single": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-01-31T17:43:40.529579" + "timestamp": "2024-02-29T08:48:55.657331" }, "multiqc_stub": { "content": [ @@ -17,25 +17,25 @@ "multiqc_report.html", "multiqc_data", "multiqc_plots", - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-01-31T17:45:09.605359" + "timestamp": "2024-02-29T08:49:49.071937" }, "multiqc_versions_config": { "content": [ [ - "versions.yml:md5,14e9a2661241abd828f4f06a7b5c222d" + "versions.yml:md5,21f35ee29416b9b3073c28733efe4b7d" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-01-31T17:44:53.535994" + "timestamp": "2024-02-29T08:49:25.457567" } } \ No newline at end of file From b7ad599a1527adffedd962f92ad1c9ba5d8db457 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 11:42:00 +0100 Subject: [PATCH 079/111] attempt ignore monochromeLogs lint --- .nf-core.yml | 2 ++ 1 file changed, 2 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 6a1c8178..6dc2a658 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -5,3 +5,5 @@ lint: - .github/ISSUE_TEMPLATE/bug_report.yml files_exist: - lib/Utils.groovy + schema_params: + - monochromeLogs From 58f5a54ed0b10ff8ecc8a2295a084d1720d94339 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 11:44:07 +0100 Subject: [PATCH 080/111] attempt ignore monochromeLogs lint --- .nf-core.yml | 3 +-- 1 file changed, 1 insertion(+), 2 deletions(-) diff --git a/.nf-core.yml b/.nf-core.yml index 6dc2a658..1f6d2308 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -5,5 +5,4 @@ lint: - .github/ISSUE_TEMPLATE/bug_report.yml files_exist: - lib/Utils.groovy - schema_params: - - monochromeLogs + schema_params: False From e87bfbf6687547476b97996bde56b0e827bdc2ab Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 11:46:27 +0100 Subject: [PATCH 081/111] Update comments --- .nf-core.yml | 2 ++ nextflow.config | 1 + 2 files changed, 3 insertions(+) diff --git a/.nf-core.yml b/.nf-core.yml index 1f6d2308..90cfd21c 100644 --- a/.nf-core.yml +++ b/.nf-core.yml @@ -5,4 +5,6 @@ lint: - .github/ISSUE_TEMPLATE/bug_report.yml files_exist: - lib/Utils.groovy + # TODO This is because of an issue with the monochromeLogs parameter + # See nextflow.config for details schema_params: False diff --git a/nextflow.config b/nextflow.config index bf24e8a9..3fe5c47f 100644 --- a/nextflow.config +++ b/nextflow.config @@ -97,6 +97,7 @@ params { // TODO temporary workaround a warning // not used anywhere and should not be necessary anymore after a nf-validation plugin update + // TODO when removing this, also remove the ignored lint check from .nf-core.yml monochromeLogs = null } From fdda7a596fc4dd733976540c05558c5efceda67d Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 12:39:28 +0100 Subject: [PATCH 082/111] Delete .nf-test.log --- .nf-test.log | 17 ----------------- 1 file changed, 17 deletions(-) delete mode 100644 .nf-test.log diff --git a/.nf-test.log b/.nf-test.log deleted file mode 100644 index ca137888..00000000 --- a/.nf-test.log +++ /dev/null @@ -1,17 +0,0 @@ -Feb-27 21:54:16.881 [main] INFO com.askimed.nf.test.App - nf-test 0.8.4 -Feb-27 21:54:16.895 [main] INFO com.askimed.nf.test.App - Arguments: [test, tests/main_pipeline_kallisto.test, --update-snapshot] -Feb-27 21:54:17.590 [main] INFO com.askimed.nf.test.App - Nextflow Version: 23.10.1 -Feb-27 21:54:17.592 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Load config from file /home/ec2-user/scrnaseq/nf-test.config... -Feb-27 21:54:17.887 [main] INFO com.askimed.nf.test.commands.RunTestsCommand - Detected 1 test files. -Feb-27 21:54:18.162 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/home/ec2-user/scrnaseq/bin' to '/home/ec2-user/scrnaseq/.nf-test/tests/72e0d0f6b834ab79755c0f97ef8c4ca8/meta/bin' -Feb-27 21:54:18.164 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/home/ec2-user/scrnaseq/lib' to '/home/ec2-user/scrnaseq/.nf-test/tests/72e0d0f6b834ab79755c0f97ef8c4ca8/meta/lib' -Feb-27 21:54:18.164 [main] INFO com.askimed.nf.test.config.FileStaging - Copy directory '/home/ec2-user/scrnaseq/assets' to '/home/ec2-user/scrnaseq/.nf-test/tests/72e0d0f6b834ab79755c0f97ef8c4ca8/meta/assets' -Feb-27 21:54:18.171 [main] DEBUG com.askimed.nf.test.core.AbstractTest - Stage 0 user provided files... -Feb-27 21:54:18.174 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Started test plan -Feb-27 21:54:18.174 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Running testsuite 'Test Workflow main.nf' from file '/home/ec2-user/scrnaseq/tests/main_pipeline_kallisto.test'. -Feb-27 21:54:18.174 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Run test '72e0d0f6: test-dataset_kallisto_aligner'. type: com.askimed.nf.test.lang.pipeline.PipelineTest -Feb-27 21:55:36.281 [main] DEBUG com.askimed.nf.test.lang.extensions.SnapshotFile - Load snapshots from file '/home/ec2-user/scrnaseq/tests/main_pipeline_kallisto.test.snap' -Feb-27 21:55:36.305 [main] DEBUG com.askimed.nf.test.lang.extensions.Snapshot - Snapshots 'test-dataset_kallisto_aligner' match. -Feb-27 21:55:36.306 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Test '72e0d0f6: test-dataset_kallisto_aligner' finished. status: PASSED -Feb-27 21:55:36.308 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Testsuite 'Test Workflow main.nf' finished. snapshot file: true, skipped tests: false, failed tests: false -Feb-27 21:55:36.308 [main] INFO com.askimed.nf.test.core.TestExecutionEngine - Executed 1 tests. 0 tests failed. Done! From 6b46540896052f43db7e91b5c4d6e95f85498013 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 12:40:45 +0100 Subject: [PATCH 083/111] Update nextflow_schema.json --- nextflow_schema.json | 1 - 1 file changed, 1 deletion(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 0f1433c4..42599d59 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -237,7 +237,6 @@ "type": "string", "description": "Specify a path to the intron transcripts-to-capture.", "fa_icon": "fas fa-rainbow" - "fa_icon": "fas fa-fish", "format": "file-path", "exists": true }, From 904743e1f1e2ed62485f10135925ad33d6b306c3 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 12:46:52 +0100 Subject: [PATCH 084/111] Fix nextflow schema --- nextflow_schema.json | 6 ++++-- 1 file changed, 4 insertions(+), 2 deletions(-) diff --git a/nextflow_schema.json b/nextflow_schema.json index 42599d59..65b56954 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -224,7 +224,9 @@ "kallisto_index": { "type": "string", "description": "Specify a path to the precomputed Kallisto index.", - "fa_icon": "fas fa-rainbow" + "fa_icon": "fas fa-rainbow", + "format": "file-path", + "exists": true }, "kb_t1c": { "type": "string", @@ -236,7 +238,7 @@ "kb_t2c": { "type": "string", "description": "Specify a path to the intron transcripts-to-capture.", - "fa_icon": "fas fa-rainbow" + "fa_icon": "fas fa-rainbow", "format": "file-path", "exists": true }, From 600619f7eeff522b36523630dc6c60a1c0fd6227 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 7 Mar 2024 12:50:46 +0100 Subject: [PATCH 085/111] Update changelog --- CHANGELOG.md | 4 +++- nextflow_schema.json | 5 ----- 2 files changed, 3 insertions(+), 6 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index b42791db..a9d9a7e7 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -5,7 +5,9 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 ## v2.6.0dev - [date] -- [[#91](https://github.com/nf-core/scrnaseq/issues/91)] - Change from pytests to nf-test +- Change from pytests to nf-test ([#291](https://github.com/nf-core/scrnaseq/pull/291)) +- Update template to v2.13.1 ([#309](https://github.com/nf-core/scrnaseq/pull/309)) +- Update to kallisto|bustools v0.28.2 ([#294](https://github.com/nf-core/scrnaseq/pull/294)) ## v2.5.1 diff --git a/nextflow_schema.json b/nextflow_schema.json index 65b56954..23f6e9b5 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -145,11 +145,6 @@ "default": "s3://ngi-igenomes/igenomes/", "fa_icon": "fas fa-cloud-download-alt", "hidden": true - }, - "txp2gene": { - "type": "string", - "description": "Kallisto or Alevin gene mapping file.", - "fa_icon": "fas map-marked-alt" } } }, From e350399798fe1b4656bea08ccf111a27b8e0757e Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Fri, 8 Mar 2024 13:37:43 +0100 Subject: [PATCH 086/111] Update bin/emptydrops_cell_calling.R gsub .gz only in end of string Co-authored-by: Gregor Sturm --- bin/emptydrops_cell_calling.R | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/bin/emptydrops_cell_calling.R b/bin/emptydrops_cell_calling.R index 989c2b34..2b4ee5e7 100755 --- a/bin/emptydrops_cell_calling.R +++ b/bin/emptydrops_cell_calling.R @@ -17,7 +17,7 @@ mtx <- readMM(fn_mtx) get_name <- function(file) { name <- as.character(basename(file)) - name <- gsub('\\.gz', '', name) + name <- gsub('\\.gz$', '', name) return(name) } From 7daa3107220be3df249c8a99715d900725fdf0cf Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Mon, 11 Mar 2024 13:31:18 +0100 Subject: [PATCH 087/111] fixing stub snippet --- modules/local/emptydrops.nf | 3 ++- 1 file changed, 2 insertions(+), 1 deletion(-) diff --git a/modules/local/emptydrops.nf b/modules/local/emptydrops.nf index fb0d9791..675819f7 100644 --- a/modules/local/emptydrops.nf +++ b/modules/local/emptydrops.nf @@ -77,6 +77,7 @@ process EMPTYDROPS_CELL_CALLING { stub: """ - touch emptydrops_filtered/* + mkdir emptydrops_filtered + touch emptydrops_filtered/empty_file """ } From c24a05d226643d0303dd957b6627f86a168333f8 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Mon, 11 Mar 2024 13:39:23 +0100 Subject: [PATCH 088/111] fix transposition snippet in new module --- bin/emptydrops_cell_calling.R | 14 ++++++++------ 1 file changed, 8 insertions(+), 6 deletions(-) diff --git a/bin/emptydrops_cell_calling.R b/bin/emptydrops_cell_calling.R index 2b4ee5e7..bebdcc33 100755 --- a/bin/emptydrops_cell_calling.R +++ b/bin/emptydrops_cell_calling.R @@ -21,14 +21,16 @@ get_name <- function(file) { return(name) } -# Check if barcodes are in columns, if not, transpose mtx -is_transposed<-FALSE -if (dim(barcodes)[1]!=dim(mtx)[2]){ - mtx<-t(mtx) - is_transposed<-TRUE - print('Matrix was tranposed.') +# transpose matrices when required +# based on code of 'mtx_to_seurat.R', only the data from kallisto and alevin would require transposition +print("Only kallisto and alevin have transposed matrices.") +if (aligner %in% c( "kallisto", "alevin" )) { + is_transposed <- TRUE +} else { + is_transposed <- FALSE } + # Call empty drops e.out <- emptyDrops(mtx) is.cell <- e.out$FDR <= 0.01 From 492598b11f30249a5b2f3ed29c2aa4840f17b7c2 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Tue, 12 Mar 2024 09:02:51 +0100 Subject: [PATCH 089/111] fixed problem on loading fasta>f from params.genome --- main.nf | 19 ++++++++++++------- workflows/scrnaseq.nf | 37 ++++++++++++++++++++----------------- 2 files changed, 32 insertions(+), 24 deletions(-) diff --git a/main.nf b/main.nf index 3471e5b0..7d8ba356 100644 --- a/main.nf +++ b/main.nf @@ -17,10 +17,9 @@ nextflow.enable.dsl = 2 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ -include { SCRNASEQ } from './workflows/scrnaseq' +include { SCRNASEQ } from './workflows/scrnaseq' include { PIPELINE_INITIALISATION } from './subworkflows/local/utils_nfcore_scrnaseq_pipeline' include { PIPELINE_COMPLETION } from './subworkflows/local/utils_nfcore_scrnaseq_pipeline' - include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_scrnaseq_pipeline' /* @@ -28,9 +27,9 @@ include { getGenomeAttribute } from './subworkflows/local/utils_nfcore_scrn GENOME PARAMETER VALUES ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ */ - -params.fasta = getGenomeAttribute('fasta') -params.gtf = getGenomeAttribute('gtf') +// we cannot modify params. here, we must load the files +ch_genome_fasta = params.genome ? file( getGenomeAttribute('fasta'), checkIfExists: true ) : [] +ch_gtf = params.genome ? file( getGenomeAttribute('gtf'), checkIfExists: true ) : [] /* ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ @@ -45,6 +44,8 @@ workflow NFCORE_SCRNASEQ { take: samplesheet // channel: samplesheet read in from --input + ch_genome_fasta + ch_gtf main: @@ -52,7 +53,9 @@ workflow NFCORE_SCRNASEQ { // WORKFLOW: Run pipeline // SCRNASEQ ( - samplesheet + samplesheet, + ch_genome_fasta, + ch_gtf ) emit: @@ -86,7 +89,9 @@ workflow { // WORKFLOW: Run main workflow // NFCORE_SCRNASEQ ( - PIPELINE_INITIALISATION.out.samplesheet + PIPELINE_INITIALISATION.out.samplesheet, + ch_genome_fasta, + ch_gtf ) // diff --git a/workflows/scrnaseq.nf b/workflows/scrnaseq.nf index b3a695f0..7171c970 100644 --- a/workflows/scrnaseq.nf +++ b/workflows/scrnaseq.nf @@ -1,18 +1,17 @@ -include { MULTIQC } from '../modules/nf-core/multiqc/main' -include { FASTQC_CHECK } from '../subworkflows/local/fastqc' -include { KALLISTO_BUSTOOLS } from '../subworkflows/local/kallisto_bustools' -include { SCRNASEQ_ALEVIN } from '../subworkflows/local/alevin' -include { STARSOLO } from '../subworkflows/local/starsolo' -include { CELLRANGER_ALIGN } from "../subworkflows/local/align_cellranger" -include { CELLRANGERARC_ALIGN } from "../subworkflows/local/align_cellrangerarc" -include { UNIVERSC_ALIGN } from "../subworkflows/local/align_universc" -include { MTX_CONVERSION } from "../subworkflows/local/mtx_conversion" -include { GTF_GENE_FILTER } from '../modules/local/gtf_gene_filter' -include { EMPTYDROPS_CELL_CALLING } from '../modules/local/emptydrops' - -include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' -include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_scrnaseq_pipeline' +include { MULTIQC } from '../modules/nf-core/multiqc/main' +include { FASTQC_CHECK } from '../subworkflows/local/fastqc' +include { KALLISTO_BUSTOOLS } from '../subworkflows/local/kallisto_bustools' +include { SCRNASEQ_ALEVIN } from '../subworkflows/local/alevin' +include { STARSOLO } from '../subworkflows/local/starsolo' +include { CELLRANGER_ALIGN } from "../subworkflows/local/align_cellranger" +include { CELLRANGERARC_ALIGN } from "../subworkflows/local/align_cellrangerarc" +include { UNIVERSC_ALIGN } from "../subworkflows/local/align_universc" +include { MTX_CONVERSION } from "../subworkflows/local/mtx_conversion" +include { GTF_GENE_FILTER } from '../modules/local/gtf_gene_filter' +include { EMPTYDROPS_CELL_CALLING } from '../modules/local/emptydrops' +include { paramsSummaryMultiqc } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { softwareVersionsToYAML } from '../subworkflows/nf-core/utils_nfcore_pipeline' +include { methodsDescriptionText } from '../subworkflows/local/utils_nfcore_scrnaseq_pipeline' include { paramsSummaryLog; paramsSummaryMap } from 'plugin/nf-validation' @@ -20,6 +19,8 @@ workflow SCRNASEQ { take: ch_fastq + ch_genome_fasta + ch_gtf main: @@ -28,9 +29,11 @@ workflow SCRNASEQ { error "Only cellranger supports `protocol = 'auto'`. Please specify the protocol manually!" } + // overwrite fasta and gtf if user provide a custom one + ch_genome_fasta = Channel.value(params.fasta ? file(params.fasta) : ch_genome_fasta) + ch_gtf = Channel.value(params.gtf ? file(params.gtf) : ch_gtf) + // general input and params - ch_genome_fasta = Channel.value(params.fasta ? file(params.fasta) : []) - ch_gtf = params.gtf ? file(params.gtf) : [] ch_transcript_fasta = params.transcript_fasta ? file(params.transcript_fasta): [] ch_motifs = params.motifs ? file(params.motifs) : [] ch_cellrangerarc_config = params.cellrangerarc_config ? file(params.cellrangerarc_config) : [] From 62076557aacdd2d293a2adf66093af7caa74beb1 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Tue, 12 Mar 2024 14:34:07 +0100 Subject: [PATCH 090/111] fixed transposition --- bin/emptydrops_cell_calling.R | 1 + 1 file changed, 1 insertion(+) diff --git a/bin/emptydrops_cell_calling.R b/bin/emptydrops_cell_calling.R index bebdcc33..23a45267 100755 --- a/bin/emptydrops_cell_calling.R +++ b/bin/emptydrops_cell_calling.R @@ -26,6 +26,7 @@ get_name <- function(file) { print("Only kallisto and alevin have transposed matrices.") if (aligner %in% c( "kallisto", "alevin" )) { is_transposed <- TRUE + mtx<-t(mtx) } else { is_transposed <- FALSE } From 8c5702ba767d278aab1cbb4d7adb0852ea8000ff Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Tue, 12 Mar 2024 14:34:13 +0100 Subject: [PATCH 091/111] fixed file used --- modules/local/emptydrops.nf | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/modules/local/emptydrops.nf b/modules/local/emptydrops.nf index 675819f7..ee9424f3 100644 --- a/modules/local/emptydrops.nf +++ b/modules/local/emptydrops.nf @@ -29,7 +29,7 @@ process EMPTYDROPS_CELL_CALLING { matrix = "counts_unfiltered/*.mtx" barcodes = "counts_unfiltered/*.barcodes.txt" - features = "counts_unfiltered/*.genes.txt" + features = "counts_unfiltered/*.genes.names.txt" } else if (params.aligner == "alevin") { From ec199126bf4a96dc05a123f20b4201b827abd68e Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Wed, 13 Mar 2024 07:40:36 +0100 Subject: [PATCH 092/111] Update modules --- modules.json | 8 +- modules/nf-core/cellrangerarc/count/main.nf | 4 +- modules/nf-core/gffread/tests/main.nf.test | 2 + .../gffread/tests/nextflow-gff3.config | 5 + .../kallistobustools/count/environment.yml | 2 +- .../nf-core/kallistobustools/count/main.nf | 32 +++-- .../nf-core/kallistobustools/count/meta.yml | 12 +- .../kallistobustools/count/tests/main.nf.test | 98 +++++++++++++ .../count/tests/main.nf.test.snap | 100 +++++++++++++ .../count/tests/nextflow.config | 5 + .../kallistobustools/count/tests/tags.yml | 2 + .../kallistobustools/ref/environment.yml | 2 +- modules/nf-core/kallistobustools/ref/main.nf | 32 ++++- modules/nf-core/kallistobustools/ref/meta.yml | 14 +- .../kallistobustools/ref/tests/main.nf.test | 120 ++++++++++++++++ .../ref/tests/main.nf.test.snap | 136 ++++++++++++++++++ .../kallistobustools/ref/tests/tags.yml | 2 + 17 files changed, 544 insertions(+), 32 deletions(-) create mode 100644 modules/nf-core/gffread/tests/nextflow-gff3.config create mode 100644 modules/nf-core/kallistobustools/count/tests/main.nf.test create mode 100644 modules/nf-core/kallistobustools/count/tests/main.nf.test.snap create mode 100644 modules/nf-core/kallistobustools/count/tests/nextflow.config create mode 100644 modules/nf-core/kallistobustools/count/tests/tags.yml create mode 100644 modules/nf-core/kallistobustools/ref/tests/main.nf.test create mode 100644 modules/nf-core/kallistobustools/ref/tests/main.nf.test.snap create mode 100644 modules/nf-core/kallistobustools/ref/tests/tags.yml diff --git a/modules.json b/modules.json index 8aa2641e..32171104 100644 --- a/modules.json +++ b/modules.json @@ -22,7 +22,7 @@ }, "cellrangerarc/count": { "branch": "master", - "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", + "git_sha": "18e53e27cfeca5dbbfbeee675c05438dec68245f", "installed_by": ["modules"] }, "cellrangerarc/mkgtf": { @@ -42,7 +42,7 @@ }, "gffread": { "branch": "master", - "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", + "git_sha": "b1b959609bda44341120aed1766329909f54b8d0", "installed_by": ["modules"] }, "gunzip": { @@ -52,12 +52,12 @@ }, "kallistobustools/count": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", "installed_by": ["modules"] }, "kallistobustools/ref": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", "installed_by": ["modules"] }, "multiqc": { diff --git a/modules/nf-core/cellrangerarc/count/main.nf b/modules/nf-core/cellrangerarc/count/main.nf index 01eac247..e5ce9225 100644 --- a/modules/nf-core/cellrangerarc/count/main.nf +++ b/modules/nf-core/cellrangerarc/count/main.nf @@ -15,8 +15,8 @@ process CELLRANGERARC_COUNT { output: tuple val(meta), path("${meta.id}/outs/**"), emit: outs - path("${meta.id}_lib.csv") , emit: lib - path "versions.yml" , emit: versions + path("${meta.id}_lib.csv") , emit: lib + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when diff --git a/modules/nf-core/gffread/tests/main.nf.test b/modules/nf-core/gffread/tests/main.nf.test index bdbc96ae..452aba1b 100644 --- a/modules/nf-core/gffread/tests/main.nf.test +++ b/modules/nf-core/gffread/tests/main.nf.test @@ -38,6 +38,8 @@ nextflow_process { test("sarscov2-gff3-gff3") { + config "./nextflow-gff3.config" + when { params { outdir = "$outputDir" diff --git a/modules/nf-core/gffread/tests/nextflow-gff3.config b/modules/nf-core/gffread/tests/nextflow-gff3.config new file mode 100644 index 00000000..afe0830e --- /dev/null +++ b/modules/nf-core/gffread/tests/nextflow-gff3.config @@ -0,0 +1,5 @@ +process { + withName: GFFREAD { + ext.args = '' + } +} diff --git a/modules/nf-core/kallistobustools/count/environment.yml b/modules/nf-core/kallistobustools/count/environment.yml index 7ff8a2da..024f0afc 100644 --- a/modules/nf-core/kallistobustools/count/environment.yml +++ b/modules/nf-core/kallistobustools/count/environment.yml @@ -4,4 +4,4 @@ channels: - bioconda - defaults dependencies: - - bioconda::kb-python=0.27.2 + - bioconda::kb-python=0.28.2 diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index 036bb35d..841ea2fe 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -4,8 +4,8 @@ process KALLISTOBUSTOOLS_COUNT { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/kb-python:0.27.2--pyhdfd78af_0' : - 'biocontainers/kb-python:0.27.2--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/kb-python:0.28.2--pyhdfd78af_2' : + 'biocontainers/kb-python:0.28.2--pyhdfd78af_2' }" input: tuple val(meta), path(reads) @@ -14,11 +14,12 @@ process KALLISTOBUSTOOLS_COUNT { path t1c path t2c val technology + val workflow_mode output: - tuple val(meta), path ("*.count"), emit: count - path "versions.yml" , emit: versions - path "*.count/*/*.mtx" , emit: matrix //Ensure that kallisto finished and produced outputs + tuple val(meta), path ("*.count") , emit: count + path "versions.yml" , emit: versions + path "*.count/*/*.mtx" , emit: matrix //Ensure that kallisto finished and produced outputs when: task.ext.when == null || task.ext.when @@ -27,7 +28,7 @@ process KALLISTOBUSTOOLS_COUNT { def args = task.ext.args ?: '' def prefix = task.ext.prefix ?: "${meta.id}" def cdna = t1c ? "-c1 $t1c" : '' - def introns = t2c ? "-c2 $t2c" : '' + def intron = t2c ? "-c2 $t2c" : '' def memory = task.memory.toGiga() - 1 """ kb \\ @@ -36,12 +37,25 @@ process KALLISTOBUSTOOLS_COUNT { -i $index \\ -g $t2g \\ $cdna \\ - $introns \\ + $intron \\ -x $technology \\ + --workflow $workflow_mode \\ $args \\ -o ${prefix}.count \\ - ${reads.join( " " )} \\ - -m ${memory}G + -m ${memory}G \\ + ${reads.join( " " )} + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kallistobustools: \$(echo \$(kb --version 2>&1) | sed 's/^.*kb_python //;s/positional arguments.*\$//') + END_VERSIONS + """ + + stub: + def prefix = task.ext.prefix ?: "${meta.id}" + """ + mkdir -p ${prefix}.count/counts_unfiltered/ + touch ${prefix}.count/counts_unfiltered/cells_x_genes.mtx cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/kallistobustools/count/meta.yml b/modules/nf-core/kallistobustools/count/meta.yml index 7491248c..55d5dc6c 100644 --- a/modules/nf-core/kallistobustools/count/meta.yml +++ b/modules/nf-core/kallistobustools/count/meta.yml @@ -12,7 +12,7 @@ tools: homepage: https://www.kallistobus.tools/ documentation: https://kb-python.readthedocs.io/en/latest/index.html tool_dev_url: https://github.com/pachterlab/kb_python - licence: MIT License + licence: ["MIT"] input: - meta: type: map @@ -34,16 +34,16 @@ input: pattern: "*t2g.txt" - t1c: type: file - description: kb ref's c1 spliced_t2c file + description: kb ref's c1 cdna_t2c file pattern: "*.{cdna_t2c.txt}" - t2c: type: file - description: kb ref's c2 unspliced_t2c file - pattern: "*.{introns_t2c.txt}" + description: kb ref's c2 intron_t2c file + pattern: "*.{intron_t2c.txt}" - workflow_mode: type: string - description: String value defining workflow to use, can be one of "standard", "lamanno", "nucleus" - pattern: "{standard,lamanno,nucleus,kite}" + description: String value defining workflow to use, can be one of "standard", "nac", "lamanno" (obsolete) + pattern: "{standard,lamanno,nac}" - technology: type: string description: String value defining the sequencing technology used. diff --git a/modules/nf-core/kallistobustools/count/tests/main.nf.test b/modules/nf-core/kallistobustools/count/tests/main.nf.test new file mode 100644 index 00000000..550001f9 --- /dev/null +++ b/modules/nf-core/kallistobustools/count/tests/main.nf.test @@ -0,0 +1,98 @@ +nextflow_process { + + name "Test Process KALLISTOBUSTOOLS_COUNT" + script "../main.nf" + process "KALLISTOBUSTOOLS_COUNT" + + tag "modules" + tag "modules_nfcore" + tag "kallistobustools" + tag "kallistobustools/count" + tag "kallistobustools/ref" + + setup { + run("KALLISTOBUSTOOLS_REF") { + script "../../ref/main.nf" + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "standard" + """ + } + } + } + + test("genome.fasta + genome.gtf + '10X3' + 'standard'") { + + when { + process { + """ + input[0] = Channel.of( + [ + [id:'test'], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R1_001.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R2_001.fastq.gz', checkIfExists: true) + ] + ] + ) + input[1] = KALLISTOBUSTOOLS_REF.out.index + input[2] = KALLISTOBUSTOOLS_REF.out.t2g + input[3] = KALLISTOBUSTOOLS_REF.out.cdna_t2c.ifEmpty{ [] } // when empty the module doesn't run unless something is passed. + input[4] = KALLISTOBUSTOOLS_REF.out.intron_t2c.ifEmpty{ [] } // when empty the module doesn't run unless something is passed. + input[5] = "10XV3" + input[6] = "standard" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + process.out.matrix, + path(process.out.count.get(0).get(1)).list().findAll { file(it.toString()).name != "run_info.json" && file(it.toString()).name != "kb_info.json" }, + file(path(process.out.count.get(0).get(1)).list().find { file(it.toString()).name == "kb_info.json" }.toString()).readLines()[15..22], + file(path(process.out.count.get(0).get(1)).list().find { file(it.toString()).name == "run_info.json" }.toString()).readLines()[0..9] + ).match() + } + ) + } + } + + test("genome.fasta + genome.gtf + '10X3' + 'standard' - stub") { + + options "-stub" + + when { + process { + """ + input[0] = Channel.of( + [ + [id:'test'], // meta map + [ + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R1_001.fastq.gz', checkIfExists: true), + file(params.modules_testdata_base_path + 'genomics/homo_sapiens/10xgenomics/cellranger/10k_pbmc_cmo/fastqs/gex_1/subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R2_001.fastq.gz', checkIfExists: true) + ] + ] + ) + input[1] = KALLISTOBUSTOOLS_REF.out.index + input[2] = KALLISTOBUSTOOLS_REF.out.t2g + input[3] = KALLISTOBUSTOOLS_REF.out.cdna_t2c.ifEmpty{ [] } // when empty the module doesn't run unless something is passed. + input[4] = KALLISTOBUSTOOLS_REF.out.intron_t2c.ifEmpty{ [] } // when empty the module doesn't run unless something is passed. + input[5] = "10XV3" + input[6] = "standard" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } +} diff --git a/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap b/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap new file mode 100644 index 00000000..3378c3c1 --- /dev/null +++ b/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap @@ -0,0 +1,100 @@ +{ + "genome.fasta + genome.gtf + '10X3' + 'standard' - stub": { + "content": [ + { + "0": [ + [ + { + "id": "test" + }, + [ + [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ] + ], + "1": [ + "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" + ], + "2": [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "count": [ + [ + { + "id": "test" + }, + [ + [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ] + ], + "matrix": [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "versions": [ + "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-01T15:48:45.775904562" + }, + "genome.fasta + genome.gtf + '10X3' + 'standard'": { + "content": [ + [ + "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" + ], + [ + "cells_x_genes.mtx:md5,e12a45e7f7d6527f698dd9cb2e99ecd1" + ], + [ + "10x_version3_whitelist.txt:md5,3d36d0a4021fd292b265e2b5e72aaaf3", + [ + "cells_x_genes.barcodes.txt:md5,41f7adaf43b60f2f4f62d6a7073688de", + "cells_x_genes.genes.names.txt:md5,b29afa75be300c7f24fbd0740a66689b", + "cells_x_genes.genes.txt:md5,fe6d5501923867b514a0447aa4b4995f", + "cells_x_genes.mtx:md5,e12a45e7f7d6527f698dd9cb2e99ecd1" + ], + "inspect.json:md5,bafb47a58ac1bbf9be953f21c361d266", + "matrix.ec:md5,31a4c1a3e8e0c562b12f6569ffbf5459", + "output.bus:md5,d6fa0612a4a16eaf8a3e08bdc13ff49c", + "output.unfiltered.bus:md5,bf899b967657f612ba864188868d58cc", + "transcripts.txt:md5,23861cf43033e7c596e6989a88a3a373" + ], + [ + " \"commands\": [", + " \"kallisto bus -i kb_ref_out.idx -o test.count -x 10XV3 -t 2 subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R1_001.fastq.gz subsampled_SC3_v3_NextGem_DI_CellPlex_Human_PBMC_10K_1_gex_S2_L001_R2_001.fastq.gz\",", + " \"bustools sort -o test.count/tmp/output.s.bus -T test.count/tmp -t 2 -m 2G test.count/output.bus\",", + " \"bustools inspect -o test.count/inspect.json -w test.count/10x_version3_whitelist.txt test.count/tmp/output.s.bus\",", + " \"bustools correct -o test.count/tmp/output.s.c.bus -w test.count/10x_version3_whitelist.txt test.count/tmp/output.s.bus\",", + " \"bustools sort -o test.count/output.unfiltered.bus -T test.count/tmp -t 2 -m 2G test.count/tmp/output.s.c.bus\",", + " \"bustools count -o test.count/counts_unfiltered/cells_x_genes -g t2g.txt -e test.count/matrix.ec -t test.count/transcripts.txt --genecounts --umi-gene test.count/output.unfiltered.bus\"", + " ]," + ], + [ + "{", + "\t\"n_targets\": 12,", + "\t\"n_bootstraps\": 0,", + "\t\"n_processed\": 10000,", + "\t\"n_pseudoaligned\": 26,", + "\t\"n_unique\": 26,", + "\t\"p_pseudoaligned\": 0.3,", + "\t\"p_unique\": 0.3,", + "\t\"kallisto_version\": \"0.50.1\",", + "\t\"index_version\": 13," + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-01T15:48:24.476953631" + } +} \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/count/tests/nextflow.config b/modules/nf-core/kallistobustools/count/tests/nextflow.config new file mode 100644 index 00000000..7a5cbfb4 --- /dev/null +++ b/modules/nf-core/kallistobustools/count/tests/nextflow.config @@ -0,0 +1,5 @@ +process { + withName: KALLISTOBUSTOOLS_COUNT { + ext.args = '--cellranger -m 1' + } +} diff --git a/modules/nf-core/kallistobustools/count/tests/tags.yml b/modules/nf-core/kallistobustools/count/tests/tags.yml new file mode 100644 index 00000000..9c432071 --- /dev/null +++ b/modules/nf-core/kallistobustools/count/tests/tags.yml @@ -0,0 +1,2 @@ +kallistobustools/count: + - "modules/nf-core/kallistobustools/count/**" diff --git a/modules/nf-core/kallistobustools/ref/environment.yml b/modules/nf-core/kallistobustools/ref/environment.yml index acbd0e0a..6ae07a8c 100644 --- a/modules/nf-core/kallistobustools/ref/environment.yml +++ b/modules/nf-core/kallistobustools/ref/environment.yml @@ -4,5 +4,5 @@ channels: - bioconda - defaults dependencies: - - bioconda::kb-python=0.27.2 + - bioconda::kb-python=0.28.2 - conda-forge::requests>=2.23.0 diff --git a/modules/nf-core/kallistobustools/ref/main.nf b/modules/nf-core/kallistobustools/ref/main.nf index 68d72ca9..0b45203d 100644 --- a/modules/nf-core/kallistobustools/ref/main.nf +++ b/modules/nf-core/kallistobustools/ref/main.nf @@ -4,8 +4,8 @@ process KALLISTOBUSTOOLS_REF { conda "${moduleDir}/environment.yml" container "${ workflow.containerEngine == 'singularity' && !task.ext.singularity_pull_docker_container ? - 'https://depot.galaxyproject.org/singularity/kb-python:0.27.2--pyhdfd78af_0' : - 'biocontainers/kb-python:0.27.2--pyhdfd78af_0' }" + 'https://depot.galaxyproject.org/singularity/kb-python:0.28.2--pyhdfd78af_2' : + 'biocontainers/kb-python:0.28.2--pyhdfd78af_2' }" input: path fasta @@ -62,4 +62,32 @@ process KALLISTOBUSTOOLS_REF { END_VERSIONS """ } + + stub: + if (workflow_mode == "standard") { + """ + touch kb_ref_out.idx \\ + touch t2g.txt \\ + touch cdna.fa + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kallistobustools: \$(echo \$(kb --version 2>&1) | sed 's/^.*kb_python //;s/positional arguments.*\$//') + END_VERSIONS + """ + } else { + """ + touch kb_ref_out.idx \\ + touch t2g.txt \\ + touch cdna.fa + touch intron.fa \\ + touch cdna_t2c.txt \\ + touch intron_t2c.txt + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + kallistobustools: \$(echo \$(kb --version 2>&1) | sed 's/^.*kb_python //;s/positional arguments.*\$//') + END_VERSIONS + """ + } } diff --git a/modules/nf-core/kallistobustools/ref/meta.yml b/modules/nf-core/kallistobustools/ref/meta.yml index 00be5143..64deab9b 100644 --- a/modules/nf-core/kallistobustools/ref/meta.yml +++ b/modules/nf-core/kallistobustools/ref/meta.yml @@ -14,7 +14,7 @@ tools: documentation: https://kb-python.readthedocs.io/en/latest/index.html tool_dev_url: https://github.com/pachterlab/kb_python doi: "10.22002/D1.1876" - licence: MIT License + licence: ["MIT"] input: - fasta: type: file @@ -26,8 +26,8 @@ input: pattern: "*.{gtf,gtf.gz}" - workflow_mode: type: string - description: String value defining workflow to use, can be one of "standard", "lamanno", "nucleus" - pattern: "{standard,lamanno,nucleus}" + description: String value defining workflow to use, can be one of "standard", "nac", "lamanno" (obsolete) + pattern: "{standard,lamanno,nac}" output: - versions: type: file @@ -43,19 +43,19 @@ output: pattern: "*t2g.{txt}" - cdna: type: file - description: Cdna fasta file + description: cDNA fasta file pattern: "*cdna.{fa}" - intron: type: file - description: intron fasta file + description: Intron fasta file pattern: "*intron.{fa}" - cdna_t2c: type: file - description: cdna transcript to capture file + description: cDNA transcript to capture file pattern: "*cdna_t2c.{txt}" - intron_t2c: type: file - description: intron transcript to capture file + description: Intron transcript to capture file pattern: "*intron_t2c.{txt}" authors: - "@flowuenne" diff --git a/modules/nf-core/kallistobustools/ref/tests/main.nf.test b/modules/nf-core/kallistobustools/ref/tests/main.nf.test new file mode 100644 index 00000000..dc49d9ac --- /dev/null +++ b/modules/nf-core/kallistobustools/ref/tests/main.nf.test @@ -0,0 +1,120 @@ +nextflow_process { + + name "Test Process KALLISTOBUSTOOLS_REF" + script "../main.nf" + process "KALLISTOBUSTOOLS_REF" + + tag "modules" + tag "modules_nfcore" + tag "kallistobustools" + tag "kallistobustools/ref" + + test("genome.fasta + genome.gtf + 'standard'") { + + when { + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "standard" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + process.out.t2g, + process.out.cdna, + process.out.intron, + process.out.cdna_t2c, + process.out.intron_t2c, + ).match() + }, + { assert file(process.out.index.get(0)).exists() } + ) + } + } + + test("genome.fasta + genome.gtf + 'nac'") { + + when { + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "nac" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + process.out.t2g, + process.out.cdna, + process.out.intron, + process.out.cdna_t2c, + process.out.intron_t2c, + ).match() + }, + { assert file(process.out.index.get(0)).exists() } + ) + } + } + + test("genome.fasta + genome.gtf + 'standard' - stub") { + + options "-stub" + + when { + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "standard" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + } + + test("genome.fasta + genome.gtf + 'nac' - stub") { + + when { + process { + """ + input[0] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.fasta', checkIfExists: true) + input[1] = file(params.modules_testdata_base_path + 'genomics/homo_sapiens/genome/genome.gtf', checkIfExists: true) + input[2] = "nac" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + process.out.versions, + process.out.t2g, + process.out.cdna, + process.out.intron, + process.out.cdna_t2c, + process.out.intron_t2c, + ).match() + }, + { assert file(process.out.index.get(0)).exists() } + ) + } + } +} diff --git a/modules/nf-core/kallistobustools/ref/tests/main.nf.test.snap b/modules/nf-core/kallistobustools/ref/tests/main.nf.test.snap new file mode 100644 index 00000000..9c2be8a7 --- /dev/null +++ b/modules/nf-core/kallistobustools/ref/tests/main.nf.test.snap @@ -0,0 +1,136 @@ +{ + "genome.fasta + genome.gtf + 'standard'": { + "content": [ + [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ], + [ + "t2g.txt:md5,d1a8a22c59b9cb0bda39c0c9bb3f6afe" + ], + [ + "cdna.fa:md5,7bca59288fab822451de250d2eee48dc" + ], + [ + + ], + [ + + ], + [ + + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T23:37:43.739374859" + }, + "genome.fasta + genome.gtf + 'nac'": { + "content": [ + [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ], + [ + "t2g.txt:md5,58591306b33bb948bac7b40f346d0cd7" + ], + [ + "cdna.fa:md5,7bca59288fab822451de250d2eee48dc" + ], + [ + "intron.fa:md5,1aad4e3f5d006f495cc6647fa0bbf6ff" + ], + [ + "cdna_t2c.txt:md5,23861cf43033e7c596e6989a88a3a373" + ], + [ + "intron_t2c.txt:md5,fe6d5501923867b514a0447aa4b4995f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T23:37:59.436989671" + }, + "genome.fasta + genome.gtf + 'standard' - stub": { + "content": [ + { + "0": [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ], + "1": [ + "kb_ref_out.idx:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "2": [ + "t2g.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "3": [ + "cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "4": [ + + ], + "5": [ + + ], + "6": [ + + ], + "cdna": [ + "cdna.fa:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "cdna_t2c": [ + + ], + "index": [ + "kb_ref_out.idx:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "intron": [ + + ], + "intron_t2c": [ + + ], + "t2g": [ + "t2g.txt:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "versions": [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T23:38:09.578411047" + }, + "genome.fasta + genome.gtf + 'nac' - stub": { + "content": [ + [ + "versions.yml:md5,d3d08c3c5638ae540965f77b8178b3c1" + ], + [ + "t2g.txt:md5,58591306b33bb948bac7b40f346d0cd7" + ], + [ + "cdna.fa:md5,7bca59288fab822451de250d2eee48dc" + ], + [ + "intron.fa:md5,1aad4e3f5d006f495cc6647fa0bbf6ff" + ], + [ + "cdna_t2c.txt:md5,23861cf43033e7c596e6989a88a3a373" + ], + [ + "intron_t2c.txt:md5,fe6d5501923867b514a0447aa4b4995f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-02-29T23:38:25.355912473" + } +} \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/ref/tests/tags.yml b/modules/nf-core/kallistobustools/ref/tests/tags.yml new file mode 100644 index 00000000..208c8d27 --- /dev/null +++ b/modules/nf-core/kallistobustools/ref/tests/tags.yml @@ -0,0 +1,2 @@ +kallistobustools/ref: + - "modules/nf-core/kallistobustools/ref/**" From e20e897866db7491bcebba0896b51d745f83e315 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Wed, 13 Mar 2024 08:06:39 +0100 Subject: [PATCH 093/111] Update CHANGELOG --- .gitignore | 1 + CHANGELOG.md | 1 + 2 files changed, 2 insertions(+) diff --git a/.gitignore b/.gitignore index 61b9470a..bc675aba 100644 --- a/.gitignore +++ b/.gitignore @@ -10,3 +10,4 @@ log/ reports/ testme.sh .nf-test* +.vscode diff --git a/CHANGELOG.md b/CHANGELOG.md index a9d9a7e7..51953318 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -8,6 +8,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Change from pytests to nf-test ([#291](https://github.com/nf-core/scrnaseq/pull/291)) - Update template to v2.13.1 ([#309](https://github.com/nf-core/scrnaseq/pull/309)) - Update to kallisto|bustools v0.28.2 ([#294](https://github.com/nf-core/scrnaseq/pull/294)) +- Fix cellrangerarc matrix conversions and protocol selection ([#300](https://github.com/nf-core/scrnaseq/pull/300)) ## v2.5.1 From 3adeb65e0cd3bdac5e3db9fd1d50bf54ee5abea2 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 13 Mar 2024 10:48:14 +0100 Subject: [PATCH 094/111] updating documentation --- docs/output.md | 26 ++++++++++++++++++++++++++ 1 file changed, 26 insertions(+) diff --git a/docs/output.md b/docs/output.md index 7e9f0cd8..aac47219 100644 --- a/docs/output.md +++ b/docs/output.md @@ -19,6 +19,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d - [Cellranger](#cellranger) - [Cellranger ARC](#cellranger-arc) - [UniverSC](#universc) + - [Custom emptydrops filter](#custom-emptydrops-filter) - [Other output data](#other-output-data) - [MultiQC](#multiqc) - [Pipeline information](#pipeline-information) @@ -128,6 +129,16 @@ Battenberg, K., Kelly, S.T., Ras, R.A., Hetherington, N.A., Hayashi, K., and Min - Contains the mapped BAM files, filtered and unfiltered HDF5 matrices and output metrics created by the open-source implementation of Cell Ranger run via UniverSC +## Custom emptydrops filter + +The pipeline also possess a module to perform empty-drops calling and filtering with a custom-made script that uses a library called `bioconductor-dropletutils` that is available in `bioconda`. The process is simple, it takes a raw/unfiltered matrix file, and performs the empty-drops calling and filtering on it, generating another matrix file. + +> Users can turn it of with `--skip_emptydrops`. + +**Output directory: `results/${params.aligner}/emptydrops_filtered`** + +- Contains the empty-drops filtered matrices results generated by the `bioconductor-dropletutils` custom script + ## Other output data **Output directory: `results/reference_genome`** @@ -143,6 +154,21 @@ Battenberg, K., Kelly, S.T., Ras, R.A., Hetherington, N.A., Hayashi, K., and Min - `*_matrix.h5ad` - `.mtx` files converted to [AnnData](https://anndata.readthedocs.io/en/latest/) in `.h5ad` format, using [scanpy package](https://scanpy.readthedocs.io/en/stable/). - One per sample and a single one with all samples concatenated together `combined_matrix.h5ad` +- `*_matrix.rds` + - `.mtx` files converted to R native data format, rds, using the [Seurat package](https://github.com/satijalab/seurat) + - One per sample + +Because the pipeline has both the data directly from the aligners, and from the custom empty-drops filtering module the conversion modules were modified to understand the difference between raw/filtered from the aligners itself and filtered from the custom empty-drops module. So, to try to avoid confusion by the user, we added "suffixes" to the generated converted files so that we have provenance from what input it came from. + +So, the conversion modules generate data with the following syntax: **`*_{raw,filtered,custom_emptydrops_filter}_matrix.{h5ad,rds}`**. With the following meanings: + +| suffix | meaning | +| :----- | :------ | +| raw | Conversion of the raw/unprocessed matrix generated by the tool. It is also used for tools that generate only one matrix, such as alevin. | +| filtered | Conversion of the filtered/processed matrix generated by the tool | +| custom_emptydrops_filter | Conversion of the matrix that was generated by the new custom empty drops filter module | + +> Some aligners, like `alevin` do not produce both raw&filtered matrices. When aligners give only one output, they are treated with the `raw` suffix. Some aligners may have an option to give both raw&filtered and only one, like `kallisto`. Be aware when using the tools. ## MultiQC From 48dd996a65391afb8842f2f51f9f0ed7ec89b2f7 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 13 Mar 2024 12:18:28 +0100 Subject: [PATCH 095/111] remove unused parameter --- nextflow.config | 1 - nextflow_schema.json | 2 +- 2 files changed, 1 insertion(+), 2 deletions(-) diff --git a/nextflow.config b/nextflow.config index a0d4f37b..8fefe42c 100644 --- a/nextflow.config +++ b/nextflow.config @@ -27,7 +27,6 @@ params { salmon_index = null // kallisto bustools parameters - kallisto_gene_map = null kallisto_index = null kb_workflow = "standard" kb_t1c = null diff --git a/nextflow_schema.json b/nextflow_schema.json index 6b2353bb..b799d78c 100644 --- a/nextflow_schema.json +++ b/nextflow_schema.json @@ -168,7 +168,7 @@ "txp2gene": { "type": "string", "description": "Path to transcript to gene mapping file. This allows the specification of a transcript to gene mapping file for Salmon Alevin and AlevinQC.", - "help_text": "> This is not the same as the `kallisto_gene_map` parameter down below and is only used by the Salmon Alevin workflow.", + "help_text": "> This is only used by the Salmon Alevin workflow.", "fa_icon": "fas fa-map-marked-alt", "format": "file-path", "exists": true From 9cd8edd7796f62a412afbcbdbe8c44e12197473e Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 13 Mar 2024 13:24:16 +0100 Subject: [PATCH 096/111] adjust modules to handle kallisto outputs form non-standard (lamanno & nac) workflows --- modules/local/emptydrops.nf | 26 ++++++++++++++++++++++---- modules/local/mtx_to_h5ad.nf | 26 ++++++++++++++++++++++---- modules/local/mtx_to_seurat.nf | 26 ++++++++++++++++++++++---- 3 files changed, 66 insertions(+), 12 deletions(-) diff --git a/modules/local/emptydrops.nf b/modules/local/emptydrops.nf index ee9424f3..907295ab 100644 --- a/modules/local/emptydrops.nf +++ b/modules/local/emptydrops.nf @@ -31,6 +31,24 @@ process EMPTYDROPS_CELL_CALLING { barcodes = "counts_unfiltered/*.barcodes.txt" features = "counts_unfiltered/*.genes.names.txt" + // kallisto allows the following workflows: ["standard", "lamanno", "nac"] + // lamanno creates "spliced" and "unspliced" + // nac creates "nascent", "ambiguous" "mature" + // also, lamanno produces a barcodes and genes file for both spliced and unspliced + // while nac keep only one for all the different .mtx files produced + kb_non_standard_files = "" + if (params.kb_workflow == "lamanno") { + kb_non_standard_files = "spliced unspliced" + matrix = "counts_unfiltered/\${input_type}.mtx" + barcodes = "counts_unfiltered/\${input_type}.barcodes.txt" + features = "counts_unfiltered/\${input_type}.genes.txt" + } + if (params.kb_workflow == "nac") { + kb_non_standard_files = "nascent ambiguous mature" + matrix = "counts_unfiltered/*\${input_type}.mtx" + features = "counts_unfiltered/*.genes.txt" + } // barcodes tsv has same pattern as standard workflow + } else if (params.aligner == "alevin") { matrix = "*_alevin_results/af_quant/alevin/quants_mat.mtx" @@ -52,11 +70,11 @@ process EMPTYDROPS_CELL_CALLING { """ mkdir emptydrops_filtered/ # convert file types - for splice_type in spliced unspliced ; do + for input_type in ${kb_non_standard_files} ; do emptydrops_cell_calling.R \\ - counts_unfiltered/\${splice_type}.mtx \\ - counts_unfiltered/\${splice_type}.barcodes.txt \\ - counts_unfiltered/\${splice_type}.genes.txt \\ + ${matrix} \\ + ${barcodes} \\ + ${features} \\ emptydrops_filtered \\ ${params.aligner} \\ 0 diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index 3b672645..c603683c 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -49,6 +49,24 @@ process MTX_TO_H5AD { barcodes_tsv = "${mtx_dir}/*.barcodes.txt" features_tsv = "${mtx_dir}/*.genes.names.txt" + // kallisto allows the following workflows: ["standard", "lamanno", "nac"] + // lamanno creates "spliced" and "unspliced" + // nac creates "nascent", "ambiguous" "mature" + // also, lamanno produces a barcodes and genes file for both spliced and unspliced + // while nac keep only one for all the different .mtx files produced + kb_non_standard_files = "" + if (params.kb_workflow == "lamanno") { + kb_non_standard_files = "spliced unspliced" + matrix = "${mtx_dir}/\${input_type}.mtx" + barcodes_tsv = "${mtx_dir}/\${input_type}.barcodes.txt" + features_tsv = "${mtx_dir}/\${input_type}.genes.txt" + } + if (params.kb_workflow == "nac") { + kb_non_standard_files = "nascent ambiguous mature" + matrix = "${mtx_dir}/*\${input_type}.mtx" + features_tsv = "${mtx_dir}/*.genes.txt" + } // barcodes tsv has same pattern as standard workflow + } else if (params.aligner == 'alevin') { // alevin does not have filtered/unfiltered results @@ -83,13 +101,13 @@ process MTX_TO_H5AD { else if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') """ # convert file types - for input_type in nascent ambiguous mature ; do + for input_type in ${kb_non_standard_files} ; do mtx_to_h5ad.py \\ --aligner ${params.aligner} \\ --sample ${meta.id} \\ - --input ${mtx_dir}/\${input_type}.mtx \\ - --barcode ${mtx_dir}/\${input_type}.barcodes.txt \\ - --feature ${mtx_dir}/\${input_type}.genes.names.txt \\ + --input ${matrix} \\ + --barcode ${barcodes_tsv} \\ + --feature ${features_tsv} \\ --txp2gene ${txp2gene} \\ --star_index ${star_index} \\ --out ${meta.id}/${meta.id}_\${input_type}_matrix.h5ad ; diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index 4944686f..3c072c28 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -50,6 +50,24 @@ process MTX_TO_SEURAT { barcodes = "${mtx_dir}/*.barcodes.txt" features = "${mtx_dir}/*.genes.names.txt" + // kallisto allows the following workflows: ["standard", "lamanno", "nac"] + // lamanno creates "spliced" and "unspliced" + // nac creates "nascent", "ambiguous" "mature" + // also, lamanno produces a barcodes and genes file for both spliced and unspliced + // while nac keep only one for all the different .mtx files produced + kb_non_standard_files = "" + if (params.kb_workflow == "lamanno") { + kb_non_standard_files = "spliced unspliced" + matrix = "${mtx_dir}/\${input_type}.mtx" + barcodes = "${mtx_dir}/\${input_type}.barcodes.txt" + features = "${mtx_dir}/\${input_type}.genes.txt" + } + if (params.kb_workflow == "nac") { + kb_non_standard_files = "nascent ambiguous mature" + matrix = "${mtx_dir}/*\${input_type}.mtx" + features = "${mtx_dir}/*.genes.txt" + } // barcodes tsv has same pattern as standard workflow + } else if (params.aligner == "alevin") { mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : '*_alevin_results/af_quant/alevin' @@ -77,11 +95,11 @@ process MTX_TO_SEURAT { if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') """ # convert file types - for input_type in nascent ambiguous mature ; do + for input_type in ${kb_non_standard_files} ; do mtx_to_seurat.R \\ - ${mtx_dir}/\${input_type}.mtx \\ - ${mtx_dir}/\${input_type}.barcodes.txt \\ - ${mtx_dir}/\${input_type}.genes.names.txt \\ + ${matrix} \\ + ${barcodes} \\ + ${features} \\ ${meta.id}/${meta.id}_\${input_type}_matrix.rds \\ ${aligner} \\ ${is_emptydrops} From d77eda82960050fdaa0b31095662e4bd79ebcdc4 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Wed, 13 Mar 2024 13:57:02 +0100 Subject: [PATCH 097/111] when running kallisto non-standard workflow store emptydrops in subdirs to avoid file collision --- modules/local/emptydrops.nf | 4 ++-- modules/local/mtx_to_h5ad.nf | 1 + modules/local/mtx_to_seurat.nf | 1 + 3 files changed, 4 insertions(+), 2 deletions(-) diff --git a/modules/local/emptydrops.nf b/modules/local/emptydrops.nf index 907295ab..e0b77435 100644 --- a/modules/local/emptydrops.nf +++ b/modules/local/emptydrops.nf @@ -68,14 +68,14 @@ process EMPTYDROPS_CELL_CALLING { // if (params.aligner == 'kallisto' && params.kb_workflow != 'standard') """ - mkdir emptydrops_filtered/ # convert file types for input_type in ${kb_non_standard_files} ; do + mkdir -p emptydrops_filtered/\${input_type} emptydrops_cell_calling.R \\ ${matrix} \\ ${barcodes} \\ ${features} \\ - emptydrops_filtered \\ + emptydrops_filtered/\${input_type} \\ ${params.aligner} \\ 0 done diff --git a/modules/local/mtx_to_h5ad.nf b/modules/local/mtx_to_h5ad.nf index c603683c..ba8a807e 100644 --- a/modules/local/mtx_to_h5ad.nf +++ b/modules/local/mtx_to_h5ad.nf @@ -45,6 +45,7 @@ process MTX_TO_H5AD { kb_pattern = (input_type == 'raw') ? 'un' : '' mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "counts_${kb_pattern}filtered" + if ((input_type == 'custom_emptydrops_filter') && (params.kb_workflow != 'standard')) { mtx_dir = 'emptydrops_filtered/\${input_type}' } // dir has subdirs for non-standard workflows mtx_matrix = "${mtx_dir}/*.mtx" barcodes_tsv = "${mtx_dir}/*.barcodes.txt" features_tsv = "${mtx_dir}/*.genes.names.txt" diff --git a/modules/local/mtx_to_seurat.nf b/modules/local/mtx_to_seurat.nf index 3c072c28..3ba636ff 100644 --- a/modules/local/mtx_to_seurat.nf +++ b/modules/local/mtx_to_seurat.nf @@ -46,6 +46,7 @@ process MTX_TO_SEURAT { kb_pattern = (input_type == 'raw') ? 'un' : '' mtx_dir = (input_type == 'custom_emptydrops_filter') ? 'emptydrops_filtered' : "counts_${kb_pattern}filtered" + if ((input_type == 'custom_emptydrops_filter') && (params.kb_workflow != 'standard')) { mtx_dir = 'emptydrops_filtered/\${input_type}' } // dir has subdirs for non-standard workflows matrix = "${mtx_dir}/*.mtx" barcodes = "${mtx_dir}/*.barcodes.txt" features = "${mtx_dir}/*.genes.names.txt" From c460f28f596836dc3038f077e84616c561768df3 Mon Sep 17 00:00:00 2001 From: fmalmeida Date: Mon, 18 Mar 2024 14:07:41 +0100 Subject: [PATCH 098/111] update modules to get them from nf-core/modules --- modules.json | 78 ++++++++++++++----- .../nf-core/cellranger/count/environment.yml | 5 -- modules/nf-core/cellranger/count/main.nf | 8 +- modules/nf-core/cellranger/count/meta.yml | 8 ++ .../cellranger/count/tests/main.nf.test.snap | 52 ++++++++++++- .../nf-core/kallistobustools/count/main.nf | 4 +- .../nf-core/kallistobustools/count/meta.yml | 8 ++ .../count/tests/main.nf.test.snap | 30 ++++++- 8 files changed, 159 insertions(+), 34 deletions(-) delete mode 100644 modules/nf-core/cellranger/count/environment.yml diff --git a/modules.json b/modules.json index 32171104..227393dd 100644 --- a/modules.json +++ b/modules.json @@ -7,78 +7,108 @@ "nf-core": { "cellranger/count": { "branch": "master", - "git_sha": "a2dfd9a0b2e192243695711c723d652959de39fc", - "installed_by": ["modules"] + "git_sha": "92ca535c5a8c0fe89eb71e649ee536bd355ce4fc", + "installed_by": [ + "modules" + ] }, "cellranger/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellranger/mkref": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellrangerarc/count": { "branch": "master", "git_sha": "18e53e27cfeca5dbbfbeee675c05438dec68245f", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellrangerarc/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "cellrangerarc/mkref": { "branch": "master", "git_sha": "4196b1b2e7ce265892f3979eabf7a9ddc030702f", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gffread": { "branch": "master", "git_sha": "b1b959609bda44341120aed1766329909f54b8d0", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "gunzip": { "branch": "master", "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "kallistobustools/count": { "branch": "master", - "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", - "installed_by": ["modules"] + "git_sha": "9d3e489286eead7dfe1010fd324904d8b698eca7", + "installed_by": [ + "modules" + ] }, "kallistobustools/ref": { "branch": "master", "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "star/genomegenerate": { "branch": "master", "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "universc": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] }, "unzip": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": ["modules"] + "installed_by": [ + "modules" + ] } } }, @@ -87,20 +117,26 @@ "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "utils_nfcore_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] }, "utils_nfvalidation_plugin": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": ["subworkflows"] + "installed_by": [ + "subworkflows" + ] } } } } } -} +} \ No newline at end of file diff --git a/modules/nf-core/cellranger/count/environment.yml b/modules/nf-core/cellranger/count/environment.yml deleted file mode 100644 index 662f747d..00000000 --- a/modules/nf-core/cellranger/count/environment.yml +++ /dev/null @@ -1,5 +0,0 @@ -name: cellranger_count -channels: - - conda-forge - - bioconda - - defaults diff --git a/modules/nf-core/cellranger/count/main.nf b/modules/nf-core/cellranger/count/main.nf index bbbe2359..1811d745 100644 --- a/modules/nf-core/cellranger/count/main.nf +++ b/modules/nf-core/cellranger/count/main.nf @@ -10,8 +10,8 @@ process CELLRANGER_COUNT { output: tuple val(meta), path("**/outs/**") , emit: outs - tuple val(meta), path("**/outs/filtered_feature_bc_matrix**"), emit: filtered // TODO: Add to nf-coew/modules before merging PR - tuple val(meta), path("**/outs/raw_feature_bc_matrix**") , emit: raw // TODO: Add to nf-coew/modules before merging PR + tuple val(meta), path("**/outs/filtered_feature_bc_matrix**"), emit: filtered + tuple val(meta), path("**/outs/raw_feature_bc_matrix**") , emit: raw path "versions.yml" , emit: versions when: @@ -34,7 +34,11 @@ process CELLRANGER_COUNT { def prefix = task.ext.prefix ?: "${meta.id}" """ mkdir -p "${prefix}/outs/" + mkdir -p "${prefix}/outs/filtered_feature_bc_matrix" + mkdir -p "${prefix}/outs/raw_feature_bc_matrix" echo "$prefix" > ${prefix}/outs/fake_file.txt + echo "$prefix" > ${prefix}/outs/filtered_feature_bc_matrix/fake_file.txt + echo "$prefix" > ${prefix}/outs/raw_feature_bc_matrix/fake_file.txt cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/cellranger/count/meta.yml b/modules/nf-core/cellranger/count/meta.yml index 1f1768a8..56244976 100644 --- a/modules/nf-core/cellranger/count/meta.yml +++ b/modules/nf-core/cellranger/count/meta.yml @@ -40,6 +40,14 @@ output: type: file description: Files containing the outputs of Cell Ranger, see official 10X Genomics documentation for a complete list pattern: "${meta.id}/outs/*" + - filtered: + type: file + description: Files containing the filtered outputs of Cell Ranger. + pattern: "**/outs/filtered_feature_bc_matrix**" + - raw: + type: file + description: Files containing the raw outputs of Cell Ranger. + pattern: "**/outs/raw_feature_bc_matrix**" - versions: type: file description: File containing software version diff --git a/modules/nf-core/cellranger/count/tests/main.nf.test.snap b/modules/nf-core/cellranger/count/tests/main.nf.test.snap index 7eafafd0..edfb304b 100644 --- a/modules/nf-core/cellranger/count/tests/main.nf.test.snap +++ b/modules/nf-core/cellranger/count/tests/main.nf.test.snap @@ -33,13 +33,61 @@ "single_end": false, "strandedness": "auto" }, - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" + [ + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d", + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d", + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" + ] ] ], "1": [ + [ + { + "id": "test_10x", + "single_end": false, + "strandedness": "auto" + }, + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" + ] + ], + "2": [ + [ + { + "id": "test_10x", + "single_end": false, + "strandedness": "auto" + }, + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" + ] + ], + "3": [ "versions.yml:md5,30cee1a9146b01c48d9b1db6bbe813b6" ], + "filtered": [ + [ + { + "id": "test_10x", + "single_end": false, + "strandedness": "auto" + }, + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" + ] + ], "outs": [ + [ + { + "id": "test_10x", + "single_end": false, + "strandedness": "auto" + }, + [ + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d", + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d", + "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" + ] + ] + ], + "raw": [ [ { "id": "test_10x", @@ -58,6 +106,6 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-05T17:16:12.322822411" + "timestamp": "2024-03-18T11:41:17.258523741" } } \ No newline at end of file diff --git a/modules/nf-core/kallistobustools/count/main.nf b/modules/nf-core/kallistobustools/count/main.nf index 1693c786..1efda00a 100644 --- a/modules/nf-core/kallistobustools/count/main.nf +++ b/modules/nf-core/kallistobustools/count/main.nf @@ -18,8 +18,8 @@ process KALLISTOBUSTOOLS_COUNT { output: tuple val(meta), path ("*.count") , emit: count - tuple val(meta), path ("*.count/counts_unfiltered"), emit: raw_counts // TODO: Add to nf-coew/modules before merging PR - tuple val(meta), path ("*.count/counts_filtered") , emit: filtered_counts, optional: true // TODO: Add to nf-coew/modules before merging PR + tuple val(meta), path ("*.count/counts_unfiltered"), emit: raw_counts + tuple val(meta), path ("*.count/counts_filtered") , emit: filtered_counts, optional: true path "versions.yml" , emit: versions path "*.count/*/*.mtx" , emit: matrix //Ensure that kallisto finished and produced outputs diff --git a/modules/nf-core/kallistobustools/count/meta.yml b/modules/nf-core/kallistobustools/count/meta.yml index 55d5dc6c..d491dffa 100644 --- a/modules/nf-core/kallistobustools/count/meta.yml +++ b/modules/nf-core/kallistobustools/count/meta.yml @@ -58,6 +58,14 @@ output: type: file description: kb count output folder pattern: "*.{count}" + - raw_counts: + type: file + description: kb raw counts output folder + pattern: "*.{count}/counts_unfiltered" + - filtered_counts: + type: file + description: kb filtered counts output folder + pattern: "*.{count}/counts_filtered" - versions: type: file description: File containing software versions diff --git a/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap b/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap index 3378c3c1..6f6b3183 100644 --- a/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap +++ b/modules/nf-core/kallistobustools/count/tests/main.nf.test.snap @@ -15,9 +15,22 @@ ] ], "1": [ - "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" + [ + { + "id": "test" + }, + [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] ], "2": [ + + ], + "3": [ + "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" + ], + "4": [ "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" ], "count": [ @@ -31,10 +44,23 @@ ] ] ] + ], + "filtered_counts": [ + ], "matrix": [ "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" ], + "raw_counts": [ + [ + { + "id": "test" + }, + [ + "cells_x_genes.mtx:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], "versions": [ "versions.yml:md5,6ec06270afe0a7572c41567160d927d9" ] @@ -44,7 +70,7 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-01T15:48:45.775904562" + "timestamp": "2024-03-18T11:38:48.980939376" }, "genome.fasta + genome.gtf + '10X3' + 'standard'": { "content": [ From 1c1f1ac67231658d81b1e079df12d686ce087bc5 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Mon, 18 Mar 2024 13:48:09 +0000 Subject: [PATCH 099/111] prettier fix --- docs/output.md | 12 ++++---- modules.json | 74 +++++++++++++------------------------------------- 2 files changed, 25 insertions(+), 61 deletions(-) diff --git a/docs/output.md b/docs/output.md index aac47219..cff2d442 100644 --- a/docs/output.md +++ b/docs/output.md @@ -158,15 +158,15 @@ The pipeline also possess a module to perform empty-drops calling and filtering - `.mtx` files converted to R native data format, rds, using the [Seurat package](https://github.com/satijalab/seurat) - One per sample -Because the pipeline has both the data directly from the aligners, and from the custom empty-drops filtering module the conversion modules were modified to understand the difference between raw/filtered from the aligners itself and filtered from the custom empty-drops module. So, to try to avoid confusion by the user, we added "suffixes" to the generated converted files so that we have provenance from what input it came from. +Because the pipeline has both the data directly from the aligners, and from the custom empty-drops filtering module the conversion modules were modified to understand the difference between raw/filtered from the aligners itself and filtered from the custom empty-drops module. So, to try to avoid confusion by the user, we added "suffixes" to the generated converted files so that we have provenance from what input it came from. So, the conversion modules generate data with the following syntax: **`*_{raw,filtered,custom_emptydrops_filter}_matrix.{h5ad,rds}`**. With the following meanings: -| suffix | meaning | -| :----- | :------ | -| raw | Conversion of the raw/unprocessed matrix generated by the tool. It is also used for tools that generate only one matrix, such as alevin. | -| filtered | Conversion of the filtered/processed matrix generated by the tool | -| custom_emptydrops_filter | Conversion of the matrix that was generated by the new custom empty drops filter module | +| suffix | meaning | +| :----------------------- | :--------------------------------------------------------------------------------------------------------------------------------------- | +| raw | Conversion of the raw/unprocessed matrix generated by the tool. It is also used for tools that generate only one matrix, such as alevin. | +| filtered | Conversion of the filtered/processed matrix generated by the tool | +| custom_emptydrops_filter | Conversion of the matrix that was generated by the new custom empty drops filter module | > Some aligners, like `alevin` do not produce both raw&filtered matrices. When aligners give only one output, they are treated with the `raw` suffix. Some aligners may have an option to give both raw&filtered and only one, like `kallisto`. Be aware when using the tools. diff --git a/modules.json b/modules.json index 227393dd..5d8614a7 100644 --- a/modules.json +++ b/modules.json @@ -8,107 +8,77 @@ "cellranger/count": { "branch": "master", "git_sha": "92ca535c5a8c0fe89eb71e649ee536bd355ce4fc", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellranger/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellranger/mkref": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/count": { "branch": "master", "git_sha": "18e53e27cfeca5dbbfbeee675c05438dec68245f", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/mkgtf": { "branch": "master", "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "cellrangerarc/mkref": { "branch": "master", "git_sha": "4196b1b2e7ce265892f3979eabf7a9ddc030702f", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "fastqc": { "branch": "master", "git_sha": "f4ae1d942bd50c5c0b9bd2de1393ce38315ba57c", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gffread": { "branch": "master", "git_sha": "b1b959609bda44341120aed1766329909f54b8d0", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "gunzip": { "branch": "master", "git_sha": "3a5fef109d113b4997c9822198664ca5f2716208", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "kallistobustools/count": { "branch": "master", "git_sha": "9d3e489286eead7dfe1010fd324904d8b698eca7", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "kallistobustools/ref": { "branch": "master", "git_sha": "de8215983defba48cd81961d620a9e844f11c7e7", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "multiqc": { "branch": "master", "git_sha": "b7ebe95761cd389603f9cc0e0dc384c0f663815a", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "star/genomegenerate": { "branch": "master", "git_sha": "a21faa6a3481af92a343a10926f59c189a2c16c9", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "universc": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] }, "unzip": { "branch": "master", "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", - "installed_by": [ - "modules" - ] + "installed_by": ["modules"] } } }, @@ -117,26 +87,20 @@ "utils_nextflow_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfcore_pipeline": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] }, "utils_nfvalidation_plugin": { "branch": "master", "git_sha": "5caf7640a9ef1d18d765d55339be751bb0969dfa", - "installed_by": [ - "subworkflows" - ] + "installed_by": ["subworkflows"] } } } } } -} \ No newline at end of file +} From 6351f76b50a8a528c11de6f2599cbc933d65265f Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Mon, 18 Mar 2024 14:52:00 +0000 Subject: [PATCH 100/111] small update as file names changed --- tests/main_pipeline_kallisto.test.snap | 16 ++++++++++------ 1 file changed, 10 insertions(+), 6 deletions(-) diff --git a/tests/main_pipeline_kallisto.test.snap b/tests/main_pipeline_kallisto.test.snap index 1eb15749..f9b9c96a 100644 --- a/tests/main_pipeline_kallisto.test.snap +++ b/tests/main_pipeline_kallisto.test.snap @@ -3,13 +3,13 @@ "content": [ { "stderr": [ - + ], "errorReport": "", "exitStatus": 0, "failed": false, "stdout": [ - + ], "errorMessage": "", "trace": { @@ -26,9 +26,13 @@ "cells_x_genes.barcodes.txt:md5,a8cf7ea4b2d075296a94bf066a64b7a4", "cells_x_genes.genes.txt:md5,acd9d00120f52031974b2add3e7521b6", "cells_x_genes.mtx:md5,abd83de117204d0a77df3c92d00cc025", - "Sample_X_matrix.rds:md5,0938f4189b7a7fd1030abfcee798741c", - "Sample_Y_matrix.rds:md5,93c12abe283ab37c5f37e5cd3cb25302" + "Sample_X_raw_matrix.rds:md5,0938f4189b7a7fd1030abfcee798741c", + "Sample_Y_raw_matrix.rds:md5,93c12abe283ab37c5f37e5cd3cb25302" ], - "timestamp": "2024-02-27T12:19:47.921508953" + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-18T14:51:42.040931572" } -} +} \ No newline at end of file From 9734aced56cffa65643668c8759ac2960d83a8c0 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Mon, 18 Mar 2024 14:55:58 +0000 Subject: [PATCH 101/111] add ending line --- .gitignore | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.gitignore b/.gitignore index 35378d07..bc675aba 100644 --- a/.gitignore +++ b/.gitignore @@ -10,4 +10,4 @@ log/ reports/ testme.sh .nf-test* -.vscode \ No newline at end of file +.vscode From 240660022119c0c1b7af9ac198e824355e5d79d8 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Mon, 18 Mar 2024 16:04:43 +0000 Subject: [PATCH 102/111] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 51953318..775841d1 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -9,6 +9,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Update template to v2.13.1 ([#309](https://github.com/nf-core/scrnaseq/pull/309)) - Update to kallisto|bustools v0.28.2 ([#294](https://github.com/nf-core/scrnaseq/pull/294)) - Fix cellrangerarc matrix conversions and protocol selection ([#300](https://github.com/nf-core/scrnaseq/pull/300)) +- Add new emptydrops calling module ([#301](https://github.com/nf-core/scrnaseq/pull/301)) ## v2.5.1 From 8802cd9a75983b5adabca01151c3605983b75cda Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 16 Apr 2024 08:53:25 +0000 Subject: [PATCH 103/111] updated modules with 'nf-core modules update' --- modules.json | 6 +- modules/nf-core/cellranger/count/main.nf | 12 +-- modules/nf-core/cellranger/count/meta.yml | 8 -- .../cellranger/count/tests/main.nf.test | 17 +++-- .../cellranger/count/tests/main.nf.test.snap | 74 ++++--------------- .../cellranger/count/tests/nextflow.config | 2 +- .../nf-core/cellranger/mkgtf/environment.yml | 5 -- modules/nf-core/cellranger/mkgtf/main.nf | 18 ++++- modules/nf-core/cellranger/mkgtf/meta.yml | 2 +- .../cellranger/mkgtf/tests/main.nf.test | 53 +++++++++++++ .../cellranger/mkgtf/tests/main.nf.test.snap | 48 ++++++++++++ .../cellranger/mkgtf/tests/nextflow.config | 21 ++++++ .../nf-core/cellranger/mkgtf/tests/tags.yml | 2 + .../nf-core/cellranger/mkref/environment.yml | 5 -- modules/nf-core/cellranger/mkref/main.nf | 21 +++++- modules/nf-core/cellranger/mkref/meta.yml | 2 +- .../cellranger/mkref/tests/main.nf.test | 72 ++++++++++++++++++ .../cellranger/mkref/tests/main.nf.test.snap | 52 +++++++++++++ .../cellranger/mkref/tests/nextflow.config | 6 ++ .../nf-core/cellranger/mkref/tests/tags.yml | 2 + 20 files changed, 324 insertions(+), 104 deletions(-) delete mode 100644 modules/nf-core/cellranger/mkgtf/environment.yml create mode 100644 modules/nf-core/cellranger/mkgtf/tests/main.nf.test create mode 100644 modules/nf-core/cellranger/mkgtf/tests/main.nf.test.snap create mode 100644 modules/nf-core/cellranger/mkgtf/tests/nextflow.config create mode 100644 modules/nf-core/cellranger/mkgtf/tests/tags.yml delete mode 100644 modules/nf-core/cellranger/mkref/environment.yml create mode 100644 modules/nf-core/cellranger/mkref/tests/main.nf.test create mode 100644 modules/nf-core/cellranger/mkref/tests/main.nf.test.snap create mode 100644 modules/nf-core/cellranger/mkref/tests/nextflow.config create mode 100644 modules/nf-core/cellranger/mkref/tests/tags.yml diff --git a/modules.json b/modules.json index 5d8614a7..50c3f5b7 100644 --- a/modules.json +++ b/modules.json @@ -7,17 +7,17 @@ "nf-core": { "cellranger/count": { "branch": "master", - "git_sha": "92ca535c5a8c0fe89eb71e649ee536bd355ce4fc", + "git_sha": "e66183d2ab6a5c2f3fd66b2bee942287cf65536c", "installed_by": ["modules"] }, "cellranger/mkgtf": { "branch": "master", - "git_sha": "575e1bc54b083fb15e7dd8b5fcc40bea60e8ce83", + "git_sha": "e66183d2ab6a5c2f3fd66b2bee942287cf65536c", "installed_by": ["modules"] }, "cellranger/mkref": { "branch": "master", - "git_sha": "3f5420aa22e00bd030a2556dfdffc9e164ec0ec5", + "git_sha": "e66183d2ab6a5c2f3fd66b2bee942287cf65536c", "installed_by": ["modules"] }, "cellrangerarc/count": { diff --git a/modules/nf-core/cellranger/count/main.nf b/modules/nf-core/cellranger/count/main.nf index 1811d745..cf94615b 100644 --- a/modules/nf-core/cellranger/count/main.nf +++ b/modules/nf-core/cellranger/count/main.nf @@ -2,17 +2,15 @@ process CELLRANGER_COUNT { tag "$meta.id" label 'process_high' - container "nf-core/cellranger:7.1.0" + container "nf-core/cellranger:8.0.0" input: tuple val(meta), path(reads, stageAs: "fastq_???/*") path reference output: - tuple val(meta), path("**/outs/**") , emit: outs - tuple val(meta), path("**/outs/filtered_feature_bc_matrix**"), emit: filtered - tuple val(meta), path("**/outs/raw_feature_bc_matrix**") , emit: raw - path "versions.yml" , emit: versions + tuple val(meta), path("**/outs/**"), emit: outs + path "versions.yml" , emit: versions when: task.ext.when == null || task.ext.when @@ -34,11 +32,7 @@ process CELLRANGER_COUNT { def prefix = task.ext.prefix ?: "${meta.id}" """ mkdir -p "${prefix}/outs/" - mkdir -p "${prefix}/outs/filtered_feature_bc_matrix" - mkdir -p "${prefix}/outs/raw_feature_bc_matrix" echo "$prefix" > ${prefix}/outs/fake_file.txt - echo "$prefix" > ${prefix}/outs/filtered_feature_bc_matrix/fake_file.txt - echo "$prefix" > ${prefix}/outs/raw_feature_bc_matrix/fake_file.txt cat <<-END_VERSIONS > versions.yml "${task.process}": diff --git a/modules/nf-core/cellranger/count/meta.yml b/modules/nf-core/cellranger/count/meta.yml index 56244976..1f1768a8 100644 --- a/modules/nf-core/cellranger/count/meta.yml +++ b/modules/nf-core/cellranger/count/meta.yml @@ -40,14 +40,6 @@ output: type: file description: Files containing the outputs of Cell Ranger, see official 10X Genomics documentation for a complete list pattern: "${meta.id}/outs/*" - - filtered: - type: file - description: Files containing the filtered outputs of Cell Ranger. - pattern: "**/outs/filtered_feature_bc_matrix**" - - raw: - type: file - description: Files containing the raw outputs of Cell Ranger. - pattern: "**/outs/raw_feature_bc_matrix**" - versions: type: file description: File containing software version diff --git a/modules/nf-core/cellranger/count/tests/main.nf.test b/modules/nf-core/cellranger/count/tests/main.nf.test index c779b89e..dc8a58a5 100644 --- a/modules/nf-core/cellranger/count/tests/main.nf.test +++ b/modules/nf-core/cellranger/count/tests/main.nf.test @@ -2,6 +2,7 @@ nextflow_process { name "Test Process CELLRANGER_COUNT" script "../main.nf" + config "./nextflow.config" process "CELLRANGER_COUNT" tag "modules" @@ -28,7 +29,7 @@ nextflow_process { """ input[0] = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) input[1] = CELLRANGER_MKGTF.out.gtf - input[2] = "homo_sapiens_chr22_reference" + input[2] = "homo_sapiens_reference" """ } } @@ -55,13 +56,13 @@ nextflow_process { assertAll( { assert process.success }, { - assert snapshot( - process.out.versions, - process.out.outs[0][1].findAll { file(it).name !in [ - 'web_summary.html', // unstable checksum - 'barcodes.tsv.gz' // empty file in test data -> would raise linting error - ]} - ).match() + assert snapshot( + process.out.versions, + process.out.outs[0][1].findAll { file(it).name !in [ + 'web_summary.html', // unstable checksum + 'barcodes.tsv.gz' // empty file in test data -> would raise linting error + ]} + ).match() }, { assert file(process.out.outs.get(0).get(1).find { file(it).name == 'web_summary.html' }).exists() }, { assert file(process.out.outs.get(0).get(1).find { file(it).name == 'barcodes.tsv.gz' }).exists() } diff --git a/modules/nf-core/cellranger/count/tests/main.nf.test.snap b/modules/nf-core/cellranger/count/tests/main.nf.test.snap index edfb304b..77d7d486 100644 --- a/modules/nf-core/cellranger/count/tests/main.nf.test.snap +++ b/modules/nf-core/cellranger/count/tests/main.nf.test.snap @@ -2,55 +2,31 @@ "10x example file": { "content": [ [ - "versions.yml:md5,ec6a0c18d2a8332e42956d07215a657c" + "versions.yml:md5,3c769adb8bc0f753eb9283b89f6541dd" ], [ - "filtered_feature_bc_matrix.h5:md5,cd440a402b661dc307356057d0dd3348", + "filtered_feature_bc_matrix.h5:md5,a875919b9d468efef9f846c95a7f53d6", "features.tsv.gz:md5,9f93621be0bede2b75596ad255607633", - "matrix.mtx.gz:md5,1736b88199c1dd71e907f9b93becc4f8", + "matrix.mtx.gz:md5,149c4055b85f5235d8b93d9e20dcaf4e", "metrics_summary.csv:md5,c295fe3ccbb6ce118482964ca9c748d4", - "molecule_info.h5:md5,6204ac0b164b6e06e65954d072afe599", - "possorted_genome_bam.bam:md5,32fe604968326d98e8c0c36ce3c5ee8d", - "possorted_genome_bam.bam.bai:md5,e37e792ca1b528e64cd57f7d70dae3ed", - "raw_feature_bc_matrix.h5:md5,8521dbdf077e029ae35e682d746eb79d", + "molecule_info.h5:md5,ea14830a187799eac6312181d05530f4", + "possorted_genome_bam.bam:md5,d249eff0cb6eebcfdb46342071ea8388", + "possorted_genome_bam.bam.bai:md5,223db5969de6cc2603eaa8fd0d58f766", + "raw_feature_bc_matrix.h5:md5,c34335a1aadd0d8cb6c770034c658889", "features.tsv.gz:md5,9f93621be0bede2b75596ad255607633", - "matrix.mtx.gz:md5,6294c0359b29a86ba7b06e07d4b8a134" + "matrix.mtx.gz:md5,6395f3c1ec7700b0c6a72e432830ffc1" ] ], "meta": { "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-06T09:30:22.104987533" + "timestamp": "2024-03-22T08:17:11.168345294" }, "10x example file - stub": { "content": [ { "0": [ - [ - { - "id": "test_10x", - "single_end": false, - "strandedness": "auto" - }, - [ - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d", - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d", - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" - ] - ] - ], - "1": [ - [ - { - "id": "test_10x", - "single_end": false, - "strandedness": "auto" - }, - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" - ] - ], - "2": [ [ { "id": "test_10x", @@ -60,34 +36,10 @@ "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" ] ], - "3": [ - "versions.yml:md5,30cee1a9146b01c48d9b1db6bbe813b6" - ], - "filtered": [ - [ - { - "id": "test_10x", - "single_end": false, - "strandedness": "auto" - }, - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" - ] + "1": [ + "versions.yml:md5,443f4d697e6c549da3d3f3cd0e55b55c" ], "outs": [ - [ - { - "id": "test_10x", - "single_end": false, - "strandedness": "auto" - }, - [ - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d", - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d", - "fake_file.txt:md5,0d98223c768861fd6af96f00148dbb8d" - ] - ] - ], - "raw": [ [ { "id": "test_10x", @@ -98,7 +50,7 @@ ] ], "versions": [ - "versions.yml:md5,30cee1a9146b01c48d9b1db6bbe813b6" + "versions.yml:md5,443f4d697e6c549da3d3f3cd0e55b55c" ] } ], @@ -106,6 +58,6 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-03-18T11:41:17.258523741" + "timestamp": "2024-03-22T08:15:18.556883641" } } \ No newline at end of file diff --git a/modules/nf-core/cellranger/count/tests/nextflow.config b/modules/nf-core/cellranger/count/tests/nextflow.config index 2859b34a..2d06e957 100644 --- a/modules/nf-core/cellranger/count/tests/nextflow.config +++ b/modules/nf-core/cellranger/count/tests/nextflow.config @@ -19,6 +19,6 @@ process { --attribute=gene_biotype:TR_C_gene' } withName: CELLRANGER_COUNT { - ext.args = '--chemistry SC3Pv3' + ext.args = '--chemistry SC3Pv3 --create-bam true' } } diff --git a/modules/nf-core/cellranger/mkgtf/environment.yml b/modules/nf-core/cellranger/mkgtf/environment.yml deleted file mode 100644 index c81ef3e4..00000000 --- a/modules/nf-core/cellranger/mkgtf/environment.yml +++ /dev/null @@ -1,5 +0,0 @@ -name: cellranger_mkgtf -channels: - - conda-forge - - bioconda - - defaults diff --git a/modules/nf-core/cellranger/mkgtf/main.nf b/modules/nf-core/cellranger/mkgtf/main.nf index e0b0dd67..6817a4dd 100644 --- a/modules/nf-core/cellranger/mkgtf/main.nf +++ b/modules/nf-core/cellranger/mkgtf/main.nf @@ -2,7 +2,7 @@ process CELLRANGER_MKGTF { tag "$gtf" label 'process_low' - container "nf-core/cellranger:7.1.0" + container "nf-core/cellranger:8.0.0" input: path gtf @@ -33,4 +33,20 @@ process CELLRANGER_MKGTF { cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' ) END_VERSIONS """ + + stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "CELLRANGER_MKGTF module does not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + def prefix = task.ext.prefix ?: "${gtf.baseName}.filtered" + """ + touch ${prefix}.gtf + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' ) + END_VERSIONS + """ } diff --git a/modules/nf-core/cellranger/mkgtf/meta.yml b/modules/nf-core/cellranger/mkgtf/meta.yml index a01e2830..282fcff0 100644 --- a/modules/nf-core/cellranger/mkgtf/meta.yml +++ b/modules/nf-core/cellranger/mkgtf/meta.yml @@ -10,7 +10,7 @@ tools: homepage: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger documentation: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_ov tool_dev_url: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_ov - licence: 10x Genomics EULA + licence: [10X Genomics EULA] input: - gtf: type: file diff --git a/modules/nf-core/cellranger/mkgtf/tests/main.nf.test b/modules/nf-core/cellranger/mkgtf/tests/main.nf.test new file mode 100644 index 00000000..bba46b45 --- /dev/null +++ b/modules/nf-core/cellranger/mkgtf/tests/main.nf.test @@ -0,0 +1,53 @@ +// nf-core modules test cellranger/mkgtf +nextflow_process { + + name "Test Process CELLRANGER_MKGTF" + script "../main.nf" + process "CELLRANGER_MKGTF" + + tag "modules" + tag "modules_nfcore" + tag "cellranger" + tag "cellranger/mkgtf" + + test("homo_sapiens - reference") { + + when { + process { + """ + input[0] = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + + test("homo_sapiens - reference - stub") { + + options "-stub" + + when { + process { + """ + input[0] = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true) + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out).match() } + ) + } + + } + +} diff --git a/modules/nf-core/cellranger/mkgtf/tests/main.nf.test.snap b/modules/nf-core/cellranger/mkgtf/tests/main.nf.test.snap new file mode 100644 index 00000000..05bea037 --- /dev/null +++ b/modules/nf-core/cellranger/mkgtf/tests/main.nf.test.snap @@ -0,0 +1,48 @@ +{ + "homo_sapiens - reference": { + "content": [ + { + "0": [ + "genome.filtered.gtf:md5,50fc877b1c53b36b3b413aff88bda48c" + ], + "1": [ + "versions.yml:md5,440fb677a34013b092e10c5daa9916e9" + ], + "gtf": [ + "genome.filtered.gtf:md5,50fc877b1c53b36b3b413aff88bda48c" + ], + "versions": [ + "versions.yml:md5,440fb677a34013b092e10c5daa9916e9" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-22T08:51:17.576906345" + }, + "homo_sapiens - reference - stub": { + "content": [ + { + "0": [ + "genome.filtered.gtf:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "1": [ + "versions.yml:md5,440fb677a34013b092e10c5daa9916e9" + ], + "gtf": [ + "genome.filtered.gtf:md5,d41d8cd98f00b204e9800998ecf8427e" + ], + "versions": [ + "versions.yml:md5,440fb677a34013b092e10c5daa9916e9" + ] + } + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-22T08:51:23.23741543" + } +} \ No newline at end of file diff --git a/modules/nf-core/cellranger/mkgtf/tests/nextflow.config b/modules/nf-core/cellranger/mkgtf/tests/nextflow.config new file mode 100644 index 00000000..4d971a1e --- /dev/null +++ b/modules/nf-core/cellranger/mkgtf/tests/nextflow.config @@ -0,0 +1,21 @@ +process { + withName: CELLRANGER_MKGTF { + ext.args = '--attribute=gene_biotype:protein_coding \ + --attribute=gene_biotype:lincRNA \ + --attribute=gene_biotype:antisense \ + --attribute=gene_biotype:IG_LV_gene \ + --attribute=gene_biotype:IG_V_gene \ + --attribute=gene_biotype:IG_V_pseudogene \ + --attribute=gene_biotype:IG_D_gene \ + --attribute=gene_biotype:IG_J_gene \ + --attribute=gene_biotype:IG_J_pseudogene \ + --attribute=gene_biotype:IG_C_gene \ + --attribute=gene_biotype:IG_C_pseudogene \ + --attribute=gene_biotype:TR_V_gene \ + --attribute=gene_biotype:TR_V_pseudogene \ + --attribute=gene_biotype:TR_D_gene \ + --attribute=gene_biotype:TR_J_gene \ + --attribute=gene_biotype:TR_J_pseudogene \ + --attribute=gene_biotype:TR_C_gene' + } +} diff --git a/modules/nf-core/cellranger/mkgtf/tests/tags.yml b/modules/nf-core/cellranger/mkgtf/tests/tags.yml new file mode 100644 index 00000000..7c95d529 --- /dev/null +++ b/modules/nf-core/cellranger/mkgtf/tests/tags.yml @@ -0,0 +1,2 @@ +cellranger/mkgtf: + - "modules/nf-core/cellranger/mkgtf/**" diff --git a/modules/nf-core/cellranger/mkref/environment.yml b/modules/nf-core/cellranger/mkref/environment.yml deleted file mode 100644 index 9ca3e88c..00000000 --- a/modules/nf-core/cellranger/mkref/environment.yml +++ /dev/null @@ -1,5 +0,0 @@ -name: cellranger_mkref -channels: - - conda-forge - - bioconda - - defaults diff --git a/modules/nf-core/cellranger/mkref/main.nf b/modules/nf-core/cellranger/mkref/main.nf index 986891b8..4325c308 100644 --- a/modules/nf-core/cellranger/mkref/main.nf +++ b/modules/nf-core/cellranger/mkref/main.nf @@ -2,7 +2,7 @@ process CELLRANGER_MKREF { tag "$fasta" label 'process_high' - container "nf-core/cellranger:7.1.0" + container "nf-core/cellranger:8.0.0" input: path fasta @@ -28,6 +28,8 @@ process CELLRANGER_MKREF { --genome=$reference_name \\ --fasta=$fasta \\ --genes=$gtf \\ + --localcores=${task.cpus} \\ + --localmem=${task.memory.toGiga()} \\ $args cat <<-END_VERSIONS > versions.yml @@ -35,4 +37,21 @@ process CELLRANGER_MKREF { cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' ) END_VERSIONS """ + + stub: + // Exit if running this module with -profile conda / -profile mamba + if (workflow.profile.tokenize(',').intersect(['conda', 'mamba']).size() >= 1) { + error "CELLRANGER_MKREF module does not support Conda. Please use Docker / Singularity / Podman instead." + } + def args = task.ext.args ?: '' + """ + mkdir $reference_name + touch ${reference_name}/empty_file + + cat <<-END_VERSIONS > versions.yml + "${task.process}": + cellranger: \$(echo \$( cellranger --version 2>&1) | sed 's/^.*[^0-9]\\([0-9]*\\.[0-9]*\\.[0-9]*\\).*\$/\\1/' ) + END_VERSIONS + """ + } diff --git a/modules/nf-core/cellranger/mkref/meta.yml b/modules/nf-core/cellranger/mkref/meta.yml index 4cd9091c..bd8ad5a6 100644 --- a/modules/nf-core/cellranger/mkref/meta.yml +++ b/modules/nf-core/cellranger/mkref/meta.yml @@ -10,7 +10,7 @@ tools: homepage: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/what-is-cell-ranger documentation: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_ov tool_dev_url: https://support.10xgenomics.com/single-cell-gene-expression/software/pipelines/latest/using/tutorial_ov - licence: 10x Genomics EULA + licence: [10X Genomics EULA] input: - fasta: type: file diff --git a/modules/nf-core/cellranger/mkref/tests/main.nf.test b/modules/nf-core/cellranger/mkref/tests/main.nf.test new file mode 100644 index 00000000..5282f3f6 --- /dev/null +++ b/modules/nf-core/cellranger/mkref/tests/main.nf.test @@ -0,0 +1,72 @@ +// nf-core modules test cellranger/mkref +nextflow_process { + + name "Test Process CELLRANGER_MKREF" + script "../main.nf" + config "./nextflow.config" + process "CELLRANGER_MKREF" + + tag "modules" + tag "modules_nfcore" + tag "cellranger" + tag "cellranger/mkref" + + test("homo_sapiens - reference") { + + when { + process { + """ + input[0] = file(params.test_data['homo_sapiens']['genome']['genome_fasta'], checkIfExists: true) + input[1] = file(params.test_data['homo_sapiens']['genome']['genome_gtf'], checkIfExists: true) + input[2] = "homo_sapiens_reference" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot( + path( "${outputDir}/homo_sapiens_reference/fasta" ), + path( "${outputDir}/homo_sapiens_reference/genes" ), + path( "${outputDir}/homo_sapiens_reference/reference.json" ), + path( "${outputDir}/homo_sapiens_reference/star/SA" ), + path( "${outputDir}/homo_sapiens_reference/star/SAindex" ), + path( "${outputDir}/homo_sapiens_reference/star/Genome" ), + path( "${outputDir}/homo_sapiens_reference/star/geneInfo.tab" ), + path( "${outputDir}/homo_sapiens_reference/star/exonGeTrInfo.tab" ), + path( "${outputDir}/homo_sapiens_reference/star/exonInfo.tab" ), + path( "${outputDir}/homo_sapiens_reference/star/sjdbList.out.tab" ) + ).match(), + { assert snapshot(process.out.versions).match("versions") } + } + ) + } + + } + + test("homo_sapiens - reference - stub") { + + options "-stub" + + when { + process { + """ + input[0] = [] + input[1] = [] + input[2] = "homo_sapiens_reference" + """ + } + } + + then { + assertAll( + { assert process.success }, + { assert snapshot(process.out.reference).match() }, + { assert snapshot(process.out.versions).match("versions") } + ) + } + + } + +} diff --git a/modules/nf-core/cellranger/mkref/tests/main.nf.test.snap b/modules/nf-core/cellranger/mkref/tests/main.nf.test.snap new file mode 100644 index 00000000..046fefbd --- /dev/null +++ b/modules/nf-core/cellranger/mkref/tests/main.nf.test.snap @@ -0,0 +1,52 @@ +{ + "homo_sapiens - reference": { + "content": [ + [ + "genome.fa:md5,f315020d899597c1b57e5fe9f60f4c3e", + "genome.fa.fai:md5,3520cd30e1b100e55f578db9c855f685" + ], + [ + "genes.gtf.gz:md5,50fc877b1c53b36b3b413aff88bda48c" + ], + "reference.json:md5,106d79d334779a0d675aa0a2623bea97", + "SA:md5,bcf3e1a855783105150b46c905465333", + "SAindex:md5,b93fb07d342e6c32a00ebc4311c0ad38", + "Genome:md5,22102926fadf5890e905ca71b2da3f35", + "geneInfo.tab:md5,8b608537307443ffaee4927d2b428805", + "exonGeTrInfo.tab:md5,72d4dd88d25e3c5b0bb72b12b4ac99a8", + "exonInfo.tab:md5,0d560290fab688b7268d88d5494bf9fe", + "sjdbList.out.tab:md5,9e4f991abbbfeb3935a2bb21b9e258f1" + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-25T09:41:24.407740353" + }, + "homo_sapiens - reference - stub": { + "content": [ + [ + [ + "empty_file:md5,d41d8cd98f00b204e9800998ecf8427e" + ] + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-25T09:41:46.135604587" + }, + "versions": { + "content": [ + [ + "versions.yml:md5,e9d88519c551d8ccbbdc6bd2673fc41f" + ] + ], + "meta": { + "nf-test": "0.8.4", + "nextflow": "23.10.1" + }, + "timestamp": "2024-03-25T09:41:46.149227444" + } +} \ No newline at end of file diff --git a/modules/nf-core/cellranger/mkref/tests/nextflow.config b/modules/nf-core/cellranger/mkref/tests/nextflow.config new file mode 100644 index 00000000..f10f7974 --- /dev/null +++ b/modules/nf-core/cellranger/mkref/tests/nextflow.config @@ -0,0 +1,6 @@ +process { + withName: CELLRANGER_MKREF { + publishDir = [ path: { "output" } ] + } +} + diff --git a/modules/nf-core/cellranger/mkref/tests/tags.yml b/modules/nf-core/cellranger/mkref/tests/tags.yml new file mode 100644 index 00000000..62b5d963 --- /dev/null +++ b/modules/nf-core/cellranger/mkref/tests/tags.yml @@ -0,0 +1,2 @@ +cellranger/mkref: + - "modules/nf-core/cellranger/mkref/**" From 6dc725b26fbeacb3070ed4703934ec04a644f964 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 16 Apr 2024 08:54:02 +0000 Subject: [PATCH 104/111] update changelog --- CHANGELOG.md | 1 + 1 file changed, 1 insertion(+) diff --git a/CHANGELOG.md b/CHANGELOG.md index 775841d1..88d56ee9 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -10,6 +10,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Update to kallisto|bustools v0.28.2 ([#294](https://github.com/nf-core/scrnaseq/pull/294)) - Fix cellrangerarc matrix conversions and protocol selection ([#300](https://github.com/nf-core/scrnaseq/pull/300)) - Add new emptydrops calling module ([#301](https://github.com/nf-core/scrnaseq/pull/301)) +- Update cellranger modules to latest version ([[#316](https://github.com/nf-core/scrnaseq/issues/316)]) ## v2.5.1 From 95a9ffaa10235fdf6c7ad5ffd5ef94267ea3ca4a Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 16 Apr 2024 08:56:59 +0000 Subject: [PATCH 105/111] parse filtered / raw channels --- subworkflows/local/align_cellranger.nf | 23 ++++++++++++++++++++++- 1 file changed, 22 insertions(+), 1 deletion(-) diff --git a/subworkflows/local/align_cellranger.nf b/subworkflows/local/align_cellranger.nf index 39e54675..2461373b 100644 --- a/subworkflows/local/align_cellranger.nf +++ b/subworkflows/local/align_cellranger.nf @@ -40,9 +40,30 @@ workflow CELLRANGER_ALIGN { ) ch_versions = ch_versions.mix(CELLRANGER_COUNT.out.versions) + // + // Split channels of raw and filtered to avoid file collision problems when loading the inputs in conversion modules. + // + ch_matrices_raw = + CELLRANGER_COUNT.out.outs.map { meta, mtx_files -> + def desired_files = [] + mtx_files.each{ + if ( it.toString().contains("raw_feature_bc_matrix") ) { desired_files.add( it ) } + } + [ meta, desired_files ] + } + + ch_matrices_filtered = + CELLRANGER_COUNT.out.outs.map { meta, mtx_files -> + def desired_files = [] + mtx_files.each{ + if ( it.toString().contains("filtered_feature_bc_matrix") ) { desired_files.add( it ) } + } + [ meta, desired_files ] + } + emit: ch_versions cellranger_out = CELLRANGER_COUNT.out.outs - cellranger_matrices = CELLRANGER_COUNT.out.filtered.mix( CELLRANGER_COUNT.out.raw ) + cellranger_matrices = ch_matrices_raw.mix( ch_matrices_filtered ) star_index = cellranger_index } From f0b309ffe1ff2307759648634d0ee09333b59d61 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 16 Apr 2024 10:13:00 +0000 Subject: [PATCH 106/111] update nf-test snaps --- conf/modules.config | 2 +- tests/main_pipeline_cellranger.test.snap | 16 ++++++++-------- 2 files changed, 9 insertions(+), 9 deletions(-) diff --git a/conf/modules.config b/conf/modules.config index b69f5f4e..49dd7b77 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -98,7 +98,7 @@ if(params.aligner == "cellranger") { path: "${params.outdir}/${params.aligner}/count", mode: params.publish_dir_mode ] - ext.args = {"--chemistry ${meta.chemistry} " + (meta.expected_cells ? "--expect-cells ${meta.expected_cells}" : '')} + ext.args = {"--chemistry ${meta.chemistry} --create-bam false " + (meta.expected_cells ? "--expect-cells ${meta.expected_cells}" : '')} time = { check_max( 240.h * task.attempt, 'time' ) } } } diff --git a/tests/main_pipeline_cellranger.test.snap b/tests/main_pipeline_cellranger.test.snap index 7767964f..ef8874f8 100644 --- a/tests/main_pipeline_cellranger.test.snap +++ b/tests/main_pipeline_cellranger.test.snap @@ -3,13 +3,13 @@ "content": [ { "stderr": [ - + ], "errorReport": "", "exitStatus": 0, "failed": false, "stdout": [ - + ], "errorMessage": "", "trace": { @@ -22,16 +22,16 @@ }, "barcodes.tsv.gz:md5,fe6e51564b4405b37ca8604a844b1f2e", "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", - "matrix.mtx.gz:md5,79471f700ec5bab852a960f0d1537705", + "matrix.mtx.gz:md5,1528b9b0fccc78dec95695928e42e710", "barcodes.tsv.gz:md5,77afe9a76631fc7b44236d3962a55aa5", "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", - "matrix.mtx.gz:md5,e224aa759250fa5730dc069bce9be253", + "matrix.mtx.gz:md5,49db721ca5d5749cf11597e82a010eb6", "barcodes.tsv.gz:md5,85da6b6e0c78dfe81af8c07c2017ab5e", "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", - "matrix.mtx.gz:md5,1bc71041a425bfcee38472bfec5997f8", + "matrix.mtx.gz:md5,96943587acf3356c5fa5038056c54c96", "barcodes.tsv.gz:md5,081f72b5252ccaf5ffd535ffbd235c4c", "features.tsv.gz:md5,99e453cb1443a3e43e99405184e51a5e", - "matrix.mtx.gz:md5,a4db04e43e650accc96361a287126a6b", + "matrix.mtx.gz:md5,58182db2706d532ec970526de3d3b70f", "Sample_X_raw_matrix.rds:md5,306a5477ace4d43d851b8389fdfeaf1f", "Sample_Y_raw_matrix.rds:md5,74b31532da4cae5a8197d690021d77fc", "Sample_X_filtered_matrix.rds:md5,f9191ba575a3ab79ada4807715f18573", @@ -41,6 +41,6 @@ "nf-test": "0.8.4", "nextflow": "23.10.1" }, - "timestamp": "2024-02-14T15:10:31.638485641" + "timestamp": "2024-04-16T09:43:45.32298954" } -} +} \ No newline at end of file From 984c101f817e1775cb3568b190d9f71551107db3 Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 16 Apr 2024 11:05:17 +0000 Subject: [PATCH 107/111] default to true --- conf/modules.config | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/conf/modules.config b/conf/modules.config index 49dd7b77..ffe2a0b6 100644 --- a/conf/modules.config +++ b/conf/modules.config @@ -98,7 +98,7 @@ if(params.aligner == "cellranger") { path: "${params.outdir}/${params.aligner}/count", mode: params.publish_dir_mode ] - ext.args = {"--chemistry ${meta.chemistry} --create-bam false " + (meta.expected_cells ? "--expect-cells ${meta.expected_cells}" : '')} + ext.args = {"--chemistry ${meta.chemistry} --create-bam true " + (meta.expected_cells ? "--expect-cells ${meta.expected_cells}" : '')} time = { check_max( 240.h * task.attempt, 'time' ) } } } From 5b3796fe800a039ca38678f5e0c00372bde564b9 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Tue, 16 Apr 2024 14:48:36 +0200 Subject: [PATCH 108/111] bump version and update changelog --- CHANGELOG.md | 5 +++-- assets/multiqc_config.yml | 4 ++-- nextflow.config | 2 +- 3 files changed, 6 insertions(+), 5 deletions(-) diff --git a/CHANGELOG.md b/CHANGELOG.md index 88d56ee9..de6ff058 100644 --- a/CHANGELOG.md +++ b/CHANGELOG.md @@ -3,8 +3,9 @@ The format is based on [Keep a Changelog](https://keepachangelog.com/en/1.0.0/) and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0.html). -## v2.6.0dev - [date] +## v2.6.0 - 2024-04-16 +- Update cellranger to v8.0.0 ([#317](https://github.com/nf-core/scrnaseq/pull/317)) - Change from pytests to nf-test ([#291](https://github.com/nf-core/scrnaseq/pull/291)) - Update template to v2.13.1 ([#309](https://github.com/nf-core/scrnaseq/pull/309)) - Update to kallisto|bustools v0.28.2 ([#294](https://github.com/nf-core/scrnaseq/pull/294)) @@ -12,7 +13,7 @@ and this project adheres to [Semantic Versioning](https://semver.org/spec/v2.0.0 - Add new emptydrops calling module ([#301](https://github.com/nf-core/scrnaseq/pull/301)) - Update cellranger modules to latest version ([[#316](https://github.com/nf-core/scrnaseq/issues/316)]) -## v2.5.1 +## v2.5.1 - 2024-01-23 - Template update to v2.12 ([#298](https://github.com/nf-core/scrnaseq/pull/298)). - Fix that cellranger workflow couldn't be run and enable CI for this workflow ([#288](https://github.com/nf-core/scrnaseq/pull/288)). diff --git a/assets/multiqc_config.yml b/assets/multiqc_config.yml index 584bae69..adfa48ba 100644 --- a/assets/multiqc_config.yml +++ b/assets/multiqc_config.yml @@ -1,7 +1,7 @@ report_comment: > - This report has been generated by the nf-core/scrnaseq + This report has been generated by the nf-core/scrnaseq analysis pipeline. For information about how to interpret these results, please see the - documentation. + documentation. report_section_order: "nf-core-scrnaseq-methods-description": order: -1000 diff --git a/nextflow.config b/nextflow.config index 8fefe42c..91c9c617 100644 --- a/nextflow.config +++ b/nextflow.config @@ -282,7 +282,7 @@ manifest { description = """Pipeline for processing 10x Genomics single cell rnaseq data""" mainScript = 'main.nf' nextflowVersion = '!>=23.04.0' - version = '2.6.0dev' + version = '2.6.0' doi = '10.5281/zenodo.3568187' } From c1e98b3ce7f406dc049aeadb56e28aaa43a61116 Mon Sep 17 00:00:00 2001 From: Gregor Sturm Date: Thu, 18 Apr 2024 08:05:52 +0200 Subject: [PATCH 109/111] Update download_pipeline.yml --- .github/workflows/download_pipeline.yml | 3 +++ 1 file changed, 3 insertions(+) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index 08622fd5..e549d01b 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -27,6 +27,9 @@ jobs: download: runs-on: ubuntu-latest steps: + - name: Disk space cleanup + uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 + - name: Install Nextflow uses: nf-core/setup-nextflow@v1 From 4efe296b0838f049bb03de22316c563b86a5cec2 Mon Sep 17 00:00:00 2001 From: nf-core-bot Date: Thu, 18 Apr 2024 06:08:14 +0000 Subject: [PATCH 110/111] [automated] Fix linting with Prettier --- .github/workflows/download_pipeline.yml | 2 +- 1 file changed, 1 insertion(+), 1 deletion(-) diff --git a/.github/workflows/download_pipeline.yml b/.github/workflows/download_pipeline.yml index e549d01b..e810c84b 100644 --- a/.github/workflows/download_pipeline.yml +++ b/.github/workflows/download_pipeline.yml @@ -29,7 +29,7 @@ jobs: steps: - name: Disk space cleanup uses: jlumbroso/free-disk-space@54081f138730dfa15788a46383842cd2f914a1be # v1.3.1 - + - name: Install Nextflow uses: nf-core/setup-nextflow@v1 From 900606fa49d134e74ba9c626ca9d11b29d32d3df Mon Sep 17 00:00:00 2001 From: Felipe Marques de Almeida Date: Tue, 30 Apr 2024 10:28:34 +0000 Subject: [PATCH 111/111] re-added celranger-arc backwards compatibility --- subworkflows/local/mtx_conversion.nf | 10 ++++++++++ 1 file changed, 10 insertions(+) diff --git a/subworkflows/local/mtx_conversion.nf b/subworkflows/local/mtx_conversion.nf index 55541f18..98e49a2e 100644 --- a/subworkflows/local/mtx_conversion.nf +++ b/subworkflows/local/mtx_conversion.nf @@ -14,6 +14,16 @@ workflow MTX_CONVERSION { main: ch_versions = Channel.empty() + // Cellranger module output contains too many files which cause path collisions, we filter to the ones we need. + // Keeping backwards compatibility with cellranger-arc. + // TODO: Adapt cellranger-arc subworkflow like cellranger to remove this snippet here. + if (params.aligner in [ 'cellrangerarc' ]) { + mtx_matrices = mtx_matrices.map { meta, mtx_files -> + [ meta, mtx_files.findAll { it.toString().contains("filtered_feature_bc_matrix") } ] + } + .filter { meta, mtx_files -> mtx_files } // Remove any that are missing the relevant files + } + // // Convert matrix to h5ad //
    Process Name \\", - " \\ Software Version
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    yaml5.4.1
    TOOL1tool10.11.9
    TOOL2tool21.9
    WorkflowNextflow
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