diff --git a/doc/api/fs.md b/doc/api/fs.md
index 123a9a554c66d7..21ac80081804b3 100644
--- a/doc/api/fs.md
+++ b/doc/api/fs.md
@@ -1077,6 +1077,9 @@ behavior is similar to `cp dir1/ dir2/`.
 <!-- YAML
 added: v22.0.0
 changes:
+  - version: REPLACEME
+    pr-url: https://github.com/nodejs/node/pull/58182
+    description: Add support for `URL` instances for `cwd` option.
   - version: v24.0.0
     pr-url: https://github.com/nodejs/node/pull/57513
     description: Marking the API stable.
@@ -1092,7 +1095,7 @@ changes:
 
 * `pattern` {string|string\[]}
 * `options` {Object}
-  * `cwd` {string} current working directory. **Default:** `process.cwd()`
+  * `cwd` {string|URL} current working directory. **Default:** `process.cwd()`
   * `exclude` {Function|string\[]} Function to filter out files/directories or a
     list of glob patterns to be excluded. If a function is provided, return
     `true` to exclude the item, `false` to include it. **Default:** `undefined`.
@@ -3136,6 +3139,9 @@ descriptor. See [`fs.utimes()`][].
 <!-- YAML
 added: v22.0.0
 changes:
+  - version: REPLACEME
+    pr-url: https://github.com/nodejs/node/pull/58182
+    description: Add support for `URL` instances for `cwd` option.
   - version: v24.0.0
     pr-url: https://github.com/nodejs/node/pull/57513
     description: Marking the API stable.
@@ -3152,7 +3158,7 @@ changes:
 * `pattern` {string|string\[]}
 
 * `options` {Object}
-  * `cwd` {string} current working directory. **Default:** `process.cwd()`
+  * `cwd` {string|URL} current working directory. **Default:** `process.cwd()`
   * `exclude` {Function|string\[]} Function to filter out files/directories or a
     list of glob patterns to be excluded. If a function is provided, return
     `true` to exclude the item, `false` to include it. **Default:** `undefined`.
@@ -5689,6 +5695,9 @@ Synchronous version of [`fs.futimes()`][]. Returns `undefined`.
 <!-- YAML
 added: v22.0.0
 changes:
+  - version: REPLACEME
+    pr-url: https://github.com/nodejs/node/pull/58182
+    description: Add support for `URL` instances for `cwd` option.
   - version: v24.0.0
     pr-url: https://github.com/nodejs/node/pull/57513
     description: Marking the API stable.
@@ -5704,7 +5713,7 @@ changes:
 
 * `pattern` {string|string\[]}
 * `options` {Object}
-  * `cwd` {string} current working directory. **Default:** `process.cwd()`
+  * `cwd` {string|URL} current working directory. **Default:** `process.cwd()`
   * `exclude` {Function|string\[]} Function to filter out files/directories or a
     list of glob patterns to be excluded. If a function is provided, return
     `true` to exclude the item, `false` to include it. **Default:** `undefined`.
diff --git a/lib/internal/fs/glob.js b/lib/internal/fs/glob.js
index def75f85e17bb2..14180f936ac1c8 100644
--- a/lib/internal/fs/glob.js
+++ b/lib/internal/fs/glob.js
@@ -38,6 +38,7 @@ const {
   hideStackFrames,
 } = require('internal/errors');
 const assert = require('internal/assert');
+const { toPathIfFileURL } = require('internal/url');
 
 let minimatch;
 function lazyMinimatch() {
@@ -268,7 +269,7 @@ class Glob {
   constructor(pattern, options = kEmptyObject) {
     validateObject(options, 'options');
     const { exclude, cwd, withFileTypes } = options;
-    this.#root = cwd ?? '.';
+    this.#root = toPathIfFileURL(cwd) ?? '.';
     this.#withFileTypes = !!withFileTypes;
     if (exclude != null) {
       validateStringArrayOrFunction(exclude, 'options.exclude');
diff --git a/test/parallel/test-fs-glob.mjs b/test/parallel/test-fs-glob.mjs
index 077c05279915b5..b3708f466379fa 100644
--- a/test/parallel/test-fs-glob.mjs
+++ b/test/parallel/test-fs-glob.mjs
@@ -4,6 +4,7 @@ import { resolve, dirname, sep, relative, join, isAbsolute } from 'node:path';
 import { mkdir, writeFile, symlink, glob as asyncGlob } from 'node:fs/promises';
 import { glob, globSync, Dirent } from 'node:fs';
 import { test, describe } from 'node:test';
+import { pathToFileURL } from 'node:url';
 import { promisify } from 'node:util';
 import assert from 'node:assert';
 
@@ -338,6 +339,39 @@ describe('fsPromises glob', function() {
   }
 });
 
+describe('glob - with file: URL as cwd', function() {
+  const promisified = promisify(glob);
+  for (const [pattern, expected] of Object.entries(patterns)) {
+    test(pattern, async () => {
+      const actual = (await promisified(pattern, { cwd: pathToFileURL(fixtureDir) })).sort();
+      const normalized = expected.filter(Boolean).map((item) => item.replaceAll('/', sep)).sort();
+      assert.deepStrictEqual(actual, normalized);
+    });
+  }
+});
+
+describe('globSync - with file: URL as cwd', function() {
+  for (const [pattern, expected] of Object.entries(patterns)) {
+    test(pattern, () => {
+      const actual = globSync(pattern, { cwd: pathToFileURL(fixtureDir) }).sort();
+      const normalized = expected.filter(Boolean).map((item) => item.replaceAll('/', sep)).sort();
+      assert.deepStrictEqual(actual, normalized);
+    });
+  }
+});
+
+describe('fsPromises.glob - with file: URL as cwd', function() {
+  for (const [pattern, expected] of Object.entries(patterns)) {
+    test(pattern, async () => {
+      const actual = [];
+      for await (const item of asyncGlob(pattern, { cwd: pathToFileURL(fixtureDir) })) actual.push(item);
+      actual.sort();
+      const normalized = expected.filter(Boolean).map((item) => item.replaceAll('/', sep)).sort();
+      assert.deepStrictEqual(actual, normalized);
+    });
+  }
+});
+
 const normalizeDirent = (dirent) => relative(fixtureDir, join(dirent.parentPath, dirent.name));
 // The call to `join()` with only one argument is important, as
 // it ensures that the proper path seperators are applied.