diff --git a/lib/internal/util/inspect.js b/lib/internal/util/inspect.js
index 78318243e65eca..6e0799153b3d40 100644
--- a/lib/internal/util/inspect.js
+++ b/lib/internal/util/inspect.js
@@ -1684,23 +1684,28 @@ function formatNumber(fn, number, numericSeparator) {
     }
     return fn(`${number}`, 'number');
   }
+
+  const numberString = String(number);
   const integer = MathTrunc(number);
-  const string = String(integer);
+
   if (integer === number) {
-    if (!NumberIsFinite(number) || StringPrototypeIncludes(string, 'e')) {
-      return fn(string, 'number');
+    if (!NumberIsFinite(number) || StringPrototypeIncludes(numberString, 'e')) {
+      return fn(numberString, 'number');
     }
-    return fn(`${addNumericSeparator(string)}`, 'number');
+    return fn(addNumericSeparator(numberString), 'number');
   }
   if (NumberIsNaN(number)) {
-    return fn(string, 'number');
+    return fn(numberString, 'number');
   }
+
+  const decimalIndex = StringPrototypeIndexOf(numberString, '.');
+  const integerPart = StringPrototypeSlice(numberString, 0, decimalIndex);
+  const fractionalPart = StringPrototypeSlice(numberString, decimalIndex + 1);
+
   return fn(`${
-    addNumericSeparator(string)
+    addNumericSeparator(integerPart)
   }.${
-    addNumericSeparatorEnd(
-      StringPrototypeSlice(String(number), string.length + 1),
-    )
+    addNumericSeparatorEnd(fractionalPart)
   }`, 'number');
 }
 
diff --git a/test/parallel/test-util-inspect.js b/test/parallel/test-util-inspect.js
index b06f6814e4985a..9cc8c20df5c887 100644
--- a/test/parallel/test-util-inspect.js
+++ b/test/parallel/test-util-inspect.js
@@ -3324,6 +3324,27 @@ assert.strictEqual(
     util.inspect(-123456789.12345678, { numericSeparator: true }),
     '-123_456_789.123_456_78'
   );
+
+  // Regression test for https://github.com/nodejs/node/issues/59376
+  // numericSeparator should work correctly for negative fractional numbers
+  {
+    // Test the exact values from the GitHub issue
+    const values = [0.1234, -0.12, -0.123, -0.1234, -1.234];
+    assert.strictEqual(
+      util.inspect(values, { numericSeparator: true }),
+      '[ 0.123_4, -0.12, -0.123, -0.123_4, -1.234 ]'
+    );
+
+    // Test individual negative fractional numbers between -1 and 0
+    assert.strictEqual(
+      util.inspect(-0.1234, { numericSeparator: true }),
+      '-0.123_4'
+    );
+    assert.strictEqual(
+      util.inspect(-0.12345, { numericSeparator: true }),
+      '-0.123_45'
+    );
+  }
 }
 
 // Regression test for https://github.com/nodejs/node/issues/41244