diff --git a/instrumentation/opentelemetry-instrumentation-flask/pyproject.toml b/instrumentation/opentelemetry-instrumentation-flask/pyproject.toml
index 180e401333..dc3f6e6116 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/pyproject.toml
+++ b/instrumentation/opentelemetry-instrumentation-flask/pyproject.toml
@@ -34,7 +34,6 @@ dependencies = [
 
 [project.optional-dependencies]
 instruments = [
-  "werkzeug < 3.0.0",
   "flask >= 1.0",
 ]
 test = [
diff --git a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
index 92ac4d9768..cd8f74aba6 100644
--- a/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
+++ b/opentelemetry-instrumentation/src/opentelemetry/instrumentation/bootstrap_gen.py
@@ -84,10 +84,6 @@
         "library": "fastapi ~= 0.58",
         "instrumentation": "opentelemetry-instrumentation-fastapi==0.44b0.dev",
     },
-    {
-        "library": "werkzeug < 3.0.0",
-        "instrumentation": "opentelemetry-instrumentation-flask==0.44b0.dev",
-    },
     {
         "library": "flask >= 1.0",
         "instrumentation": "opentelemetry-instrumentation-flask==0.44b0.dev",