diff --git a/instrumentation/opentelemetry-instrumentation-asgi/src/opentelemetry/instrumentation/asgi/__init__.py b/instrumentation/opentelemetry-instrumentation-asgi/src/opentelemetry/instrumentation/asgi/__init__.py
index 6ae6ce1790..4492d4e1f8 100644
--- a/instrumentation/opentelemetry-instrumentation-asgi/src/opentelemetry/instrumentation/asgi/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-asgi/src/opentelemetry/instrumentation/asgi/__init__.py
@@ -513,7 +513,7 @@ def __init__(
         )
         self.duration_histogram = self.meter.create_histogram(
             name=MetricInstruments.HTTP_SERVER_DURATION,
-            unit="ms",
+            unit="s",
             description="measures the duration of the inbound HTTP request",
         )
         self.server_response_size_histogram = self.meter.create_histogram(
@@ -609,7 +609,7 @@ async def __call__(self, scope, receive, send):
                 target = _collect_target_attribute(scope)
                 if target:
                     duration_attrs[SpanAttributes.HTTP_TARGET] = target
-                duration = max(round((default_timer() - start) * 1000), 0)
+                duration = max(default_timer() - start, 0)
                 self.duration_histogram.record(duration, duration_attrs)
                 self.active_requests_counter.add(
                     -1, active_requests_count_attrs
diff --git a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
index 583f1adeb6..4714ad8976 100644
--- a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/__init__.py
@@ -321,7 +321,7 @@ def _instrument(self, **kwargs):
         )
         _DjangoMiddleware._duration_histogram = meter.create_histogram(
             name=MetricInstruments.HTTP_SERVER_DURATION,
-            unit="ms",
+            unit="s",
             description="measures the duration of the inbound http request",
         )
         _DjangoMiddleware._active_request_counter = meter.create_up_down_counter(
diff --git a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/otel_middleware.py b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/otel_middleware.py
index 491e78cab5..f4fcbcf5b1 100644
--- a/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/otel_middleware.py
+++ b/instrumentation/opentelemetry-instrumentation-django/src/opentelemetry/instrumentation/django/middleware/otel_middleware.py
@@ -377,9 +377,7 @@ def process_response(self, request, response):
                 activation.__exit__(None, None, None)
 
         if request_start_time is not None:
-            duration = max(
-                round((default_timer() - request_start_time) * 1000), 0
-            )
+            duration = max(default_timer() - request_start_time, 0)
             self._duration_histogram.record(duration, duration_attrs)
         self._active_request_counter.add(-1, active_requests_count_attrs)
         if request.META.get(self._environ_token, None) is not None:
diff --git a/instrumentation/opentelemetry-instrumentation-falcon/src/opentelemetry/instrumentation/falcon/__init__.py b/instrumentation/opentelemetry-instrumentation-falcon/src/opentelemetry/instrumentation/falcon/__init__.py
index d6cf8249a4..a241acd53e 100644
--- a/instrumentation/opentelemetry-instrumentation-falcon/src/opentelemetry/instrumentation/falcon/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-falcon/src/opentelemetry/instrumentation/falcon/__init__.py
@@ -267,7 +267,7 @@ def __init__(self, *args, **kwargs):
         )
         self.duration_histogram = self._otel_meter.create_histogram(
             name=MetricInstruments.HTTP_SERVER_DURATION,
-            unit="ms",
+            unit="s",
             description="measures the duration of the inbound HTTP request",
         )
         self.active_requests_counter = self._otel_meter.create_up_down_counter(
@@ -385,7 +385,7 @@ def _start_response(status, response_headers, *args, **kwargs):
                 duration_attrs[
                     SpanAttributes.HTTP_STATUS_CODE
                 ] = span.attributes.get(SpanAttributes.HTTP_STATUS_CODE)
-            duration = max(round((default_timer() - start) * 1000), 0)
+            duration = max(default_timer() - start, 0)
             self.duration_histogram.record(duration, duration_attrs)
             self.active_requests_counter.add(-1, active_requests_count_attrs)
             if exception is None:
diff --git a/instrumentation/opentelemetry-instrumentation-flask/src/opentelemetry/instrumentation/flask/__init__.py b/instrumentation/opentelemetry-instrumentation-flask/src/opentelemetry/instrumentation/flask/__init__.py
index 18b4713eaf..e47704cec3 100644
--- a/instrumentation/opentelemetry-instrumentation-flask/src/opentelemetry/instrumentation/flask/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-flask/src/opentelemetry/instrumentation/flask/__init__.py
@@ -354,7 +354,7 @@ def _start_response(status, response_headers, *args, **kwargs):
             return start_response(status, response_headers, *args, **kwargs)
 
         result = wsgi_app(wrapped_app_environ, _start_response)
-        duration = max(round((default_timer() - start) * 1000), 0)
+        duration = max((default_timer() - start), 0)
         duration_histogram.record(duration, duration_attrs)
         active_requests_counter.add(-1, active_requests_count_attrs)
         return result
@@ -502,7 +502,7 @@ def __init__(self, *args, **kwargs):
         )
         duration_histogram = meter.create_histogram(
             name=MetricInstruments.HTTP_SERVER_DURATION,
-            unit="ms",
+            unit="s",
             description="measures the duration of the inbound HTTP request",
         )
         active_requests_counter = meter.create_up_down_counter(
@@ -608,7 +608,7 @@ def instrument_app(
             )
             duration_histogram = meter.create_histogram(
                 name=MetricInstruments.HTTP_SERVER_DURATION,
-                unit="ms",
+                unit="s",
                 description="measures the duration of the inbound HTTP request",
             )
             active_requests_counter = meter.create_up_down_counter(
diff --git a/instrumentation/opentelemetry-instrumentation-pyramid/src/opentelemetry/instrumentation/pyramid/callbacks.py b/instrumentation/opentelemetry-instrumentation-pyramid/src/opentelemetry/instrumentation/pyramid/callbacks.py
index e3675fcfab..2ee8a5e7b0 100644
--- a/instrumentation/opentelemetry-instrumentation-pyramid/src/opentelemetry/instrumentation/pyramid/callbacks.py
+++ b/instrumentation/opentelemetry-instrumentation-pyramid/src/opentelemetry/instrumentation/pyramid/callbacks.py
@@ -139,7 +139,7 @@ def trace_tween_factory(handler, registry):
     )
     duration_histogram = meter.create_histogram(
         name=MetricInstruments.HTTP_SERVER_DURATION,
-        unit="ms",
+        unit="s",
         description="measures the duration of the inbound HTTP request",
     )
     active_requests_counter = meter.create_up_down_counter(
@@ -197,7 +197,7 @@ def trace_tween(request):
             status = "500 InternalServerError"
             raise
         finally:
-            duration = max(round((default_timer() - start) * 1000), 0)
+            duration = max(default_timer() - start, 0)
             status = getattr(response, "status", status)
             status_code = otel_wsgi._parse_status_code(status)
             if status_code is not None:
diff --git a/instrumentation/opentelemetry-instrumentation-tornado/src/opentelemetry/instrumentation/tornado/__init__.py b/instrumentation/opentelemetry-instrumentation-tornado/src/opentelemetry/instrumentation/tornado/__init__.py
index dfa4b217df..a99a320d99 100644
--- a/instrumentation/opentelemetry-instrumentation-tornado/src/opentelemetry/instrumentation/tornado/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-tornado/src/opentelemetry/instrumentation/tornado/__init__.py
@@ -295,7 +295,7 @@ def _create_server_histograms(meter) -> Dict[str, Histogram]:
     histograms = {
         MetricInstruments.HTTP_SERVER_DURATION: meter.create_histogram(
             name=MetricInstruments.HTTP_SERVER_DURATION,
-            unit="ms",
+            unit="s",
             description="measures the duration outbound HTTP requests",
         ),
         MetricInstruments.HTTP_SERVER_REQUEST_SIZE: meter.create_histogram(
@@ -592,9 +592,7 @@ def _record_prepare_metrics(server_histograms, handler):
 
 
 def _record_on_finish_metrics(server_histograms, handler, error=None):
-    elapsed_time = round(
-        (default_timer() - server_histograms[_START_TIME]) * 1000
-    )
+    elapsed_time = (default_timer() - server_histograms[_START_TIME])
 
     response_size = int(handler._headers.get("Content-Length", 0))
     metric_attributes = _create_metric_attributes(handler)
diff --git a/instrumentation/opentelemetry-instrumentation-wsgi/src/opentelemetry/instrumentation/wsgi/__init__.py b/instrumentation/opentelemetry-instrumentation-wsgi/src/opentelemetry/instrumentation/wsgi/__init__.py
index 87c73cc737..e705f5384d 100644
--- a/instrumentation/opentelemetry-instrumentation-wsgi/src/opentelemetry/instrumentation/wsgi/__init__.py
+++ b/instrumentation/opentelemetry-instrumentation-wsgi/src/opentelemetry/instrumentation/wsgi/__init__.py
@@ -506,7 +506,7 @@ def __init__(
         )
         self.duration_histogram = self.meter.create_histogram(
             name=MetricInstruments.HTTP_SERVER_DURATION,
-            unit="ms",
+            unit="s",
             description="measures the duration of the inbound HTTP request",
         )
         self.active_requests_counter = self.meter.create_up_down_counter(
@@ -592,7 +592,7 @@ def __call__(self, environ, start_response):
                 context.detach(token)
             raise
         finally:
-            duration = max(round((default_timer() - start) * 1000), 0)
+            duration = max(default_timer() - start, 0)
             self.duration_histogram.record(duration, duration_attrs)
             self.active_requests_counter.add(-1, active_requests_count_attrs)