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Hello, may I ask if Primer3's command-line parameters support setting replacement mutations for specific waypoints? The example is as follows: I tried to set the SEQUENCE-PRIMER sequence to include the mutated base, but encountered the following error: PRIMER PICKING PRODUCS FOR mutation_deample INPUT PROBLEM: Specified left primer not in sequence
#82
opened Nov 19, 2024 by
azhe1234
A question about ntthal to cacualte "ANY" bounds between two oligos
#64
opened Mar 11, 2022 by
zenghp88
ProTip!
Exclude everything labeled
bug
with -label:bug.