diff --git a/tests/functional/legacy_api/test_removed.py b/tests/functional/legacy_api/test_removed.py
index a8140efdda87..15a6a13aa7d0 100644
--- a/tests/functional/legacy_api/test_removed.py
+++ b/tests/functional/legacy_api/test_removed.py
@@ -18,3 +18,11 @@ def test_removed_upload_apis(webtest, action):
     resp = webtest.post("/pypi?:action={}".format(action), status=410)
     assert resp.status == \
         "410 This API is no longer supported, instead simply upload the file."
+
+
+def test_remove_doc_upload(webtest):
+    resp = webtest.post("/pypi?:action=doc_upload", status=410)
+    assert resp.status == (
+        "410 Uploading documentation is no longer supported, we recommend "
+        "using https://readthedocs.org/."
+    )
diff --git a/tests/unit/legacy/api/test_pypi.py b/tests/unit/legacy/api/test_pypi.py
index 64d8399ad43d..2111cccf679e 100644
--- a/tests/unit/legacy/api/test_pypi.py
+++ b/tests/unit/legacy/api/test_pypi.py
@@ -1151,6 +1151,16 @@ def test_submit(pyramid_request):
         "410 This API is no longer supported, instead simply upload the file."
 
 
+def test_doc_upload(pyramid_request):
+    resp = pypi.doc_upload(pyramid_request)
+
+    assert resp.status_code == 410
+    assert resp.status == (
+        "410 Uploading documentation is no longer supported, we recommend "
+        "using https://readthedocs.org/."
+    )
+
+
 def test_forbidden_legacy():
     exc, request = pretend.stub(), pretend.stub()
     resp = pypi.forbidden_legacy(exc, request)
diff --git a/tests/unit/test_routes.py b/tests/unit/test_routes.py
index 078c93b2463f..f671309c3ab5 100644
--- a/tests/unit/test_routes.py
+++ b/tests/unit/test_routes.py
@@ -95,4 +95,5 @@ def add_pypi_action_route(name, action, **kwargs):
         pretend.call("legacy.api.pypi.file_upload", "file_upload"),
         pretend.call("legacy.api.pypi.submit", "submit"),
         pretend.call("legacy.api.pypi.submit_pkg_info", "submit_pkg_info"),
+        pretend.call("legacy.api.pypi.doc_upload", "doc_upload"),
     ]
diff --git a/warehouse/legacy/api/pypi.py b/warehouse/legacy/api/pypi.py
index a981cc6b9ffa..a69c0789979c 100644
--- a/warehouse/legacy/api/pypi.py
+++ b/warehouse/legacy/api/pypi.py
@@ -696,6 +696,18 @@ def submit(request):
     )
 
 
+@view_config(
+    route_name="legacy.api.pypi.doc_upload",
+    decorator=[require_POST, csrf_exempt],
+)
+def doc_upload(request):
+    return _exc_with_message(
+        HTTPGone,
+        "Uploading documentation is no longer supported, we recommend using "
+        "https://readthedocs.org/.",
+    )
+
+
 @forbidden_view_config(request_param=":action")
 def forbidden_legacy(exc, request):
     # We're not going to do anything amazing here, this just exists to override
diff --git a/warehouse/routes.py b/warehouse/routes.py
index 76422b6c6f82..070809e922bd 100644
--- a/warehouse/routes.py
+++ b/warehouse/routes.py
@@ -67,6 +67,7 @@ def includeme(config):
         "legacy.api.pypi.submit_pkg_info",
         "submit_pkg_info",
     )
+    config.add_pypi_action_route("legacy.api.pypi.doc_upload", "doc_upload")
 
     # Legacy Documentation
     config.add_route("legacy.docs", config.registry.settings["docs.url"])