diff --git a/stdlib/os/__init__.pyi b/stdlib/os/__init__.pyi
index f1017984529c..961858ce3c19 100644
--- a/stdlib/os/__init__.pyi
+++ b/stdlib/os/__init__.pyi
@@ -388,6 +388,8 @@ class DirEntry(Generic[AnyStr]):
     def __fspath__(self) -> AnyStr: ...
     if sys.version_info >= (3, 9):
         def __class_getitem__(cls, item: Any) -> GenericAlias: ...
+    if sys.version_info >= (3, 12):
+        def is_junction(self) -> bool: ...
 
 @final
 class statvfs_result(structseq[int], tuple[int, int, int, int, int, int, int, int, int, int, int]):
diff --git a/tests/stubtest_allowlists/darwin-py312.txt b/tests/stubtest_allowlists/darwin-py312.txt
index a2ca63a7655d..3c63d5b66008 100644
--- a/tests/stubtest_allowlists/darwin-py312.txt
+++ b/tests/stubtest_allowlists/darwin-py312.txt
@@ -8,7 +8,6 @@ os.PRIO_DARWIN_BG
 os.PRIO_DARWIN_NONUI
 os.PRIO_DARWIN_PROCESS
 os.PRIO_DARWIN_THREAD
-posix.DirEntry.is_junction
 posix.PRIO_DARWIN_BG
 posix.PRIO_DARWIN_NONUI
 posix.PRIO_DARWIN_PROCESS
diff --git a/tests/stubtest_allowlists/linux-py312.txt b/tests/stubtest_allowlists/linux-py312.txt
index ce665e57638f..da263d35c34d 100644
--- a/tests/stubtest_allowlists/linux-py312.txt
+++ b/tests/stubtest_allowlists/linux-py312.txt
@@ -13,7 +13,6 @@ _posixsubprocess.fork_exec
 (os|posix).CLONE_SYSVSEM
 (os|posix).CLONE_THREAD
 (os|posix).CLONE_VM
-(os|posix).DirEntry.is_junction
 (os|posix).EFD_CLOEXEC
 (os|posix).EFD_NONBLOCK
 (os|posix).EFD_SEMAPHORE
diff --git a/tests/stubtest_allowlists/py312.txt b/tests/stubtest_allowlists/py312.txt
index 8e585797d3ea..106cb339e894 100644
--- a/tests/stubtest_allowlists/py312.txt
+++ b/tests/stubtest_allowlists/py312.txt
@@ -33,7 +33,6 @@ importlib.resources.files
 importlib.util.module_for_loader
 importlib.util.set_loader
 importlib.util.set_package
-os.DirEntry.is_junction
 pathlib.Path.__init__
 pathlib.PurePath.__init__
 pathlib.PurePath.is_relative_to